An antioxidant liver-soothing and qi-regulating depression-relieving composition containing dadehua and a preparation process thereof

Abstract

The present application relates to the technical field of health food, and particularly discloses an antioxidant liver-soothing and qi-regulating and depression-relieving composition containing daye flower and a preparation process thereof, which comprises raw materials: daye flower extract, aromatic polyphenol microcapsules, rose, fried jujube kernel, white chrysanthemum, bergamot, resistant dextrin, fructooligosaccharide, composite additive and deionized water. The daye flower is modified by enzymolysis combined with plant lactic acid bacteria fermentation, so as to improve the utilization rate of effective components; the aromatic polyphenol is embedded by using composite wall materials, so as to enhance the stability thereof; and the components synergize with each other, so as to play the effects of antioxidant, liver-soothing and qi-regulating, and mood-easing. The preparation method comprises the following steps: water decoction extraction of rose and other raw materials, then blending with daye flower extract, aromatic polyphenol microcapsules and auxiliary materials, homogenization, degassing and sterilization, and finally preparation. The composition prepared by the present application has high effective component retention rate, stable system and good palatability, and is suitable for the preparation of food, health food or pharmaceutical composition.

Description

Technical Field

[0001] This invention relates to the field of health food technology, specifically to an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom and its preparation process. Background Technology

[0002] With the fast pace of life, increased mental stress, and changes in dietary structure, sub-health issues caused by emotional distress, liver qi stagnation, and elevated oxidative stress levels are receiving increasing attention. Traditional Chinese medicine theory holds that the liver governs the free flow of qi, and impaired liver qi circulation can affect mood, sleep, and the digestive system. Modern research also shows that prolonged stress is often accompanied by increased free radical production and decreased antioxidant defense capabilities. Therefore, developing natural-sourced compositions that combine antioxidant, liver-soothing, and mood-regulating effects has significant application value.

[0003] Bitter orange blossom (Citrus aurantium) is the dried flower bud or flower of the bitter orange plant (Citrus aurantium var. chinensis), belonging to the Rutaceae family. It has a fragrant aroma and is traditionally used to regulate qi, relieve chest congestion, and soothe the stomach and stop vomiting. It contains flavonoids, polyphenols, and volatile components, possessing certain antioxidant and aromatic properties. However, existing bitter orange blossom products are mostly in the form of tea, ordinary water extracts, or simple powders, resulting in insufficient release of active ingredients. Volatile aromatic components are easily lost during high-temperature extraction, concentration, and drying, leading to insufficient product efficacy stability and sensory quality.

[0004] Meanwhile, existing compound products with liver-soothing and mood-lifting or antioxidant functions typically focus on simple compounding of raw materials, lacking segmented extraction and protection of the physicochemical properties of different active components, making it difficult to effectively retain flavonoids, polyphenols, and volatile components. Although some products incorporate raw materials such as rose, bergamot, and jujube seed, most have not systematically designed the synergistic effects between components, the conversion and utilization of effective ingredients, and quality control indicators, still suffering from problems such as low component utilization, poor batch-to-batch stability, and unclear functional targeting.

[0005] Based on the above statements, the present invention provides an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom and its preparation process. Summary of the Invention

[0006] To address the problems of low utilization rate of effective ingredients, poor retention rate of volatile aromatic components, insufficient synergistic effect of functional components, poor batch-to-batch stability, and difficulty in achieving standardized production in existing liver-soothing, qi-regulating, and depression-relieving compositions, this invention provides an antioxidant liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom and its preparation process.

[0007] In a first aspect, the present invention provides an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom, employing the following technical solution: An antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange flower, comprising the following raw materials in parts by weight: 25-35 parts bitter orange flower extract, 4-8 parts aromatic polyphenol microcapsules, 8-12 parts rose petals, 10-15 parts roasted jujube seed, 5-10 parts Hangzhou white chrysanthemum, 3-5 parts bergamot, 10-15 parts resistant dextrin, 4-8 parts fructooligosaccharides, 1-2 parts compound additives, and 20-40 parts deionized water.

[0008] Preferably, the method for preparing the bitter orange flower extract is as follows: The bitter orange flower was crushed and sieved to obtain bitter orange pollen. The bitter orange pollen was added to deionized water, stirred evenly, the pH was adjusted, and a compound enzyme was added for enzymatic hydrolysis. After the enzymatic hydrolysis was completed, the enzyme was inactivated. After cooling, plant lactic acid bacteria were inoculated for fermentation. After the fermentation was completed, the mixture was filtered, the filtrate was collected and concentrated, and then spray-dried to obtain bitter orange flower extract.

[0009] Preferably, the specific preparation method of the bitter orange extract is as follows: After pulverizing the bitter orange flowers and passing them through a 40-80 mesh sieve, bitter orange pollen is obtained. The pollen is added to deionized water at a mass ratio of 1:8-15, and stirred at 200-400 rpm for 15-25 minutes. The pH is adjusted to 4.5-5.5 with 0.1-0.3 mol / L citric acid solution. A compound enzyme is added, and enzymatic hydrolysis is performed at 40-50℃ for 2-3 hours. After hydrolysis, the temperature is raised to 85-95℃ to inactivate the enzyme for 5-15 minutes, and then cooled to 30-37℃. The inoculum size is 10... 6 -10 8 CFU / g of plant lactic acid bacteria were inoculated and fermented at 30-37℃ for 16-24h. After fermentation, the mixture was filtered through a 100-200 mesh nylon filter cloth, and the filtrate was collected. The filtrate was concentrated under reduced pressure to a solid content of 15-25%, and then spray-dried at an inlet air temperature of 150-180℃ and an outlet air temperature of 75-90℃ until the moisture content was ≤5% to obtain the bitter orange flower extract.

[0010] Preferably, the compound enzyme is composed of cellulase with an enzyme activity of 20,000-30,000 U / g, pectinase with an enzyme activity of 30,000-50,000 U / g, and β-glucosidase with an enzyme activity of 10,000-20,000 U / g in a mass ratio of 1-3:1-3:0.2-1; the amount of the compound enzyme added is 0.8-1.2% of the mass of the bitter orange flower.

[0011] Preferably, the method for preparing the aromatic polyphenol microcapsules is as follows: The bitter orange blossom, Buddha's hand citron, and rose petals were mixed, pulverized, and sieved to obtain a mixed powder. The mixed powder was added to deionized water, ultrasonically extracted, and filtered to obtain an aromatic polyphenol extract. The aromatic polyphenol extract was placed in a concentration tank equipped with a condensation recovery device and concentrated under reduced pressure to obtain an aromatic polyphenol concentrate. A composite wall material was added to the aromatic polyphenol concentrate, stirred evenly, and the pH was adjusted. Then, calcium chloride solution was added dropwise for gelation. After homogenization and drying, aromatic polyphenol microcapsules were obtained.

[0012] Preferably, the specific preparation method of the aromatic polyphenol microcapsules is as follows: Mix bitter orange blossom, Buddha's hand citron, and rose petals at a mass ratio of 3-6:1-3:1-2, pulverize, and pass through a 40-80 mesh sieve to obtain a mixed powder. Add the mixed powder to deionized water at a mass ratio of 1:8-15, and extract ultrasonically at 40-50℃ and 300-500W for 20-40 minutes. Filter with a 100-200 mesh nylon filter cloth to obtain an aromatic polyphenol extract. Place the aromatic polyphenol extract in a concentration tank equipped with a condenser recovery device and concentrate under reduced pressure at 60-80℃ and -0.06MPa to -0.09MPa to 25-35% of the original volume to obtain an aromatic polyphenol concentrate. Then, at a mass ratio of 20-3... Add composite wall material to the concentrated aromatic polyphenol solution at a ratio of 0:1, stir at 200-400 rpm for 15-25 min, adjust the pH to 4.0-5.5 with 0.1-0.3 mol / L citric acid solution, add calcium chloride solution at a mass ratio of 1:20-30 to the concentrated aromatic polyphenol solution, and then add 3-5% calcium chloride solution dropwise. Stir at 20-40℃ and 300-500 rpm for 40-60 min to gel. After homogenization at 20-40 MPa 1-3 times, spray dry at an inlet air temperature of 150-180℃ and an outlet air temperature of 75-90℃ until the water content is ≤5% to obtain aromatic polyphenol microcapsules.

[0013] Preferably, the composite wall material is composed of sodium alginate, oligochitosan, and gum arabic in a mass ratio of 1-2:0.5-1:1-3.

[0014] Preferably, the composite additive is composed of mogroside, erythritol and citric acid in a mass ratio of 1:5-15:0.2-1.

[0015] Secondly, the present invention provides a preparation process for an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom, using the following technical solution: A preparation process for an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom includes the following preparation steps: S1. Weigh out rose petals, roasted jujube seeds, Hangzhou white chrysanthemum and Buddha's hand according to the weight parts, crush them separately and mix them, add deionized water for extraction, filter, collect the extract and concentrate to obtain compound plant concentrate. S2. Heat the deionized water, then add the compound plant concentrate, resistant dextrin, fructooligosaccharides and compound additives, and stir evenly to obtain the mixed base liquid. S3. Cool the mixed base liquid, add bitter orange extract and aromatic polyphenol microcapsules, stir evenly, homogenize, degas under vacuum and sterilize to obtain an antioxidant liver-soothing and qi-regulating composition containing bitter orange.

[0016] Preferably, in step S1, the material is pulverized to 40-80 mesh.

[0017] Preferably, in step S1, the extraction temperature is 70-90℃, the extraction time is 1-2 hours, and the extraction is performed 1-2 times.

[0018] Preferably, in step S1, the concentration is carried out under reduced pressure, and the concentration is carried out until the solid content is 10-20%.

[0019] Preferably, in step S2, the temperature is heated to 40-50°C; the stirring speed is 400-600 rpm; and the stirring time is 20-40 min.

[0020] Preferably, in step S3, the temperature is cooled to 25-35°C; the stirring speed is 400-600 rpm; and the stirring time is 20-40 min.

[0021] Preferably, in step S3, the homogenization pressure is 20-40 MPa, and the number of homogenization cycles is 1-3; the vacuum degree of vacuum degassing is -0.07 MPa to -0.09 MPa, and the vacuum degassing time is 10-20 min.

[0022] Preferably, the sterilization in step S3 is ultra-high temperature instantaneous sterilization, with a sterilization temperature of 115-135℃ and a sterilization time of 5-15s.

[0023] Thirdly, the present invention provides the application of an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom in the preparation of health foods, special dietary foods, or pharmaceutical compositions related to anti-oxidation, liver-soothing, mood easing, and stress improvement.

[0024] In summary, the present invention has the following beneficial effects: 1. Improve the utilization rate of effective components in bitter orange blossom: This invention treats bitter orange blossom with compound enzyme hydrolysis and plant lactic acid bacteria fermentation. The cellulase, pectinase and β-glucosidase in the compound enzyme can synergistically destroy the cell wall structure of bitter orange blossom plant, promoting the release of active ingredients such as flavonoids and polyphenols. Plant lactic acid bacteria fermentation can further improve the solubility, dispersibility and flavor characteristics of bitter orange blossom extract, thereby improving the utilization rate of effective components in bitter orange blossom.

[0025] 2. Improve the stability of volatile aromatic components and polyphenols: This invention uses bitter orange, bergamot and rose to prepare aromatic polyphenol microcapsules. Low-temperature ultrasonic extraction reduces the damage of high temperature to aromatic components. Sodium alginate, oligochitosan, gum arabic and calcium chloride form a gel network structure to encapsulate and protect aromatic components and polyphenols, which helps to reduce the loss of volatile components and improve the stability of the product during processing and storage.

[0026] 3. Enhancing the Synergistic Effects of Antioxidant and Liver-Soothing and Qi-Regulating Effects of the Composition: This invention uses bitter orange extract and aromatic polyphenol microcapsules as the core, combined with rose, roasted jujube seed, Hangzhou white chrysanthemum and bergamot. Among them, bitter orange, bergamot and rose are aromatic, regulate qi, soothe the liver and relieve depression, and serve as the main ingredients; roasted jujube seed nourishes the heart and calms the mind, improving poor sleep caused by emotional distress; Hangzhou white chrysanthemum clears the liver and improves eyesight, calms liver yang, and helps relieve liver stagnation and fire; bergamot and bitter orange synergistically enhance the qi-regulating effect, while rose helps to invigorate blood and promote qi circulation. The synergistic effect of each component makes the composition have the application value of antioxidation, liver-soothing and qi-regulating, mood-relieving and stress-reducing effects.

[0027] 4. Good palatability and suitable for standardized production: By adding resistant dextrin, fructooligosaccharides and a compound additive composed of mogrosides, erythritol and citric acid, this invention can improve the taste, sweet and sour balance and drinking experience of the composition. At the same time, the preparation process parameters adopted by this invention are clearly defined, including steps such as extraction, concentration, blending, homogenization, vacuum degassing and sterilization, which facilitates the control of product quality, improves batch-to-batch stability, and is suitable for industrial and standardized production. Detailed Implementation

[0028] The present invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the scope of the invention.

[0029] Where specific techniques or conditions are not specified in the examples, they shall be performed in accordance with the techniques or conditions described in the literature in this field, or in accordance with the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased through legitimate channels.

[0030] Unless otherwise specified, the experimental methods used in the following embodiments are conventional methods. Unless otherwise specified, the experimental materials used in the following embodiments are commercially available products.

[0031] Among them, plant lactic acid bacteria ( Lactiplantibacillus plantarum Purchased from China Industrial Microbial Culture Collection Center, strain preservation number: CICC 21790; Cellulase Sichuan Youlunduohui Biotechnology Co., Ltd.; Pectinase was purchased from Xi'an Haotian Biotechnology Co., Ltd. β-glucosidase was purchased from Zhengzhou Yuhe Food Additives Co., Ltd. The oligochitosan was purchased from Xi'an Haotian Bioengineering Co., Ltd. The mogrosides were purchased from Xi'an Haotian Biotechnology Co., Ltd.

[0032] Example 1 An antioxidant composition containing bitter orange flower that soothes the liver, regulates qi, and relieves depression comprises the following raw materials in parts by weight: 25 parts bitter orange flower extract, 4 parts aromatic polyphenol microcapsules, 8 parts rose petals, 10 parts roasted jujube seed, 5 parts Hangzhou white chrysanthemum, 3 parts bergamot, 10 parts resistant dextrin, 4 parts fructooligosaccharides, 1 part compound additive, and 20 parts deionized water.

[0033] The specific preparation method of the bitter orange flower extract is as follows: The bitter orange flowers were pulverized and passed through a 40-mesh sieve to obtain bitter orange pollen. The pollen was added to deionized water at a mass ratio of 1:8, and stirred at 200 rpm for 15 minutes at room temperature. The pH was adjusted to 4.5 with 0.1 mol / L citric acid solution. A complex enzyme (composed of cellulase with 20,000 U / g activity, pectinase with 30,000 U / g activity, and β-glucosidase with 10,000 U / g activity, in a mass ratio of 1:1:0.2) was added at 0.8% of the bitter orange flower mass, and enzymatic hydrolysis was performed at 40℃ for 2 hours. After hydrolysis, the temperature was raised to 85℃ for 5 minutes to inactivate the enzyme, then cooled to 30℃. The inoculum was then prepared at a rate of 10... 6 CFU / g of plant lactic acid bacteria were inoculated and fermented at 30℃ for 16h. After fermentation, the mixture was filtered through a 100-mesh nylon filter cloth, and the filtrate was collected. The filtrate was concentrated at 50℃ and -0.05MPa reduced pressure until the solid content was 15%. Then, it was spray-dried at an inlet air temperature of 150℃ and an outlet air temperature of 75℃ until the water content was 5% to obtain the bitter orange flower extract.

[0034] The specific preparation method of aromatic polyphenol microcapsules is as follows: The bitter orange blossom, Buddha's hand citron, and rose petals were mixed at a mass ratio of 3:1:1, pulverized, and passed through a 40-mesh sieve to obtain a mixed powder. The mixed powder was then added to deionized water at a mass ratio of 1:8 and ultrasonically extracted at 40℃ and 300W for 20 minutes. The extract was filtered through a 100-mesh nylon filter cloth to obtain an aromatic polyphenol extract. This extract was placed in a concentration tank equipped with a condenser recovery device and concentrated under reduced pressure at 60℃ and -0.06MPa to 25% of its original volume to obtain a concentrated aromatic polyphenol extract. The final product was then prepared at a mass ratio of 20... 1. Add composite wall material to the aromatic polyphenol concentrate, stir at 200 rpm for 15 min, adjust the pH to 4.0 with 0.1 mol / L citric acid solution, add 3% calcium chloride solution dropwise at a mass ratio of 1:20 to the aromatic polyphenol concentrate, stir at 20℃ and 300 rpm for 40 min to gel, homogenize once at 20 MPa, and then spray dry at an inlet air temperature of 150℃ and an outlet air temperature of 75℃ until the water content is 5% to obtain aromatic polyphenol microcapsules.

[0035] The compound additive consists of mogroside, erythritol and citric acid in a mass ratio of 1:5:0.2.

[0036] A preparation process for an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom includes the following preparation steps: S1. Weigh out rose petals, roasted jujube seeds, Hangzhou white chrysanthemum and Buddha's hand according to the weight parts, grind them separately to 40 mesh and mix them, add 50wt% deionized water, extract at 70℃ for 1h, filter with 100 mesh nylon filter cloth, collect the extract and concentrate it at 50℃ and -0.06MPa to the solid content of 10% to obtain compound plant concentrate. S2. Heat 50wt% deionized water to 40℃, then add compound plant concentrate, resistant dextrin, oligofructose and compound additives, and stir at 400rpm for 20min to obtain mixed base liquid. S3. Cool the mixed base liquid to 25°C, add bitter orange flower extract and aromatic polyphenol microcapsules, stir at 400 rpm for 20 min, homogenize once at 20 MPa, degas at -0.07 MPa for 10 min, and sterilize at 115°C for 5 s to obtain an antioxidant liver-soothing, qi-regulating and depression-relieving composition containing bitter orange flower.

[0037] Example 2 An antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange flower, comprising the following raw materials in parts by weight: 30 parts bitter orange flower extract, 6 parts aromatic polyphenol microcapsules, 10 parts rose petals, 12 parts roasted jujube seed, 8 parts Hangzhou white chrysanthemum, 4 parts bergamot, 12 parts resistant dextrin, 6 parts fructooligosaccharides, 1.5 parts compound additives, and 30 parts deionized water.

[0038] The specific preparation method of the bitter orange flower extract is as follows: The bitter orange flowers were pulverized and passed through a 60-mesh sieve to obtain bitter orange pollen. The pollen was added to deionized water at a mass ratio of 1:12, and stirred at 300 rpm for 20 min at room temperature. The pH was adjusted to 5.0 with 0.2 mol / L citric acid solution. A complex enzyme (composed of cellulase with 25000 U / g activity, pectinase with 40000 U / g activity, and β-glucosidase with 15000 U / g activity in a mass ratio of 2:2:0.5) was added at 1.0% of the bitter orange flower mass, and enzymatic hydrolysis was performed at 45℃ for 2.5 h. After hydrolysis, the temperature was raised to 90℃ for 10 min to inactivate the enzyme, then cooled to 35℃. The inoculum was then prepared at a rate of 10... 7 CFU / g of plant lactic acid bacteria were inoculated and fermented at 35℃ for 20h. After fermentation, the mixture was filtered through a 150-mesh nylon filter cloth, and the filtrate was collected. The filtrate was concentrated at 60℃ and -0.06MPa under reduced pressure until the solid content was 20%. Then, it was spray-dried at an inlet air temperature of 170℃ and an outlet air temperature of 80℃ until the water content was 5% to obtain the bitter orange flower extract.

[0039] The specific preparation method of aromatic polyphenol microcapsules is as follows: The bitter orange blossom, Buddha's hand citron, and rose petals were mixed at a mass ratio of 5:2:1.5, pulverized, and passed through a 60-mesh sieve to obtain a mixed powder. The mixed powder was added to deionized water at a mass ratio of 1:12 and extracted ultrasonically at 45℃ and 400W for 30 minutes. The extract was then filtered through a 150-mesh nylon filter cloth to obtain an aromatic polyphenol extract. This extract was placed in a concentration tank equipped with a condenser recovery device and concentrated under reduced pressure at 70℃ and -0.08MPa to 30% of its original volume to obtain a concentrated aromatic polyphenol solution. The mass ratio was then... The composite wall material was added to the aromatic polyphenol concentrate at a ratio of 25:1, and the mixture was stirred at 300 rpm for 20 min. The pH was adjusted to 5 with 0.2 mol / L citric acid solution. The calcium chloride solution was added dropwise at a mass ratio of 1:25 to the aromatic polyphenol concentrate. The mixture was stirred at 30℃ and 400 rpm for 50 min to gel. After homogenization twice at 30 MPa, the mixture was spray-dried at an inlet air temperature of 170℃ and an outlet air temperature of 85℃ until the water content was 5%, thus obtaining aromatic polyphenol microcapsules.

[0040] The compound additive consists of mogroside, erythritol and citric acid in a mass ratio of 1:10:0.5.

[0041] A preparation process for an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom includes the following preparation steps: S1. Weigh out rose petals, roasted jujube seeds, Hangzhou white chrysanthemum and Buddha's hand according to the weight parts, grind them separately to 60 mesh and mix them, add 50wt% deionized water, extract at 80℃ for 1.5h, filter with 150 mesh nylon filter cloth, collect the extract and concentrate at 60℃ and -0.07MPa to the solid content of 15% to obtain compound plant concentrate. S2. Heat 50wt% deionized water to 45℃, then add compound plant concentrate, resistant dextrin, oligofructose and compound additives, and stir at 500rpm for 30min to obtain mixed base liquid. S3. Cool the mixed base liquid to 30℃, add bitter orange flower extract and aromatic polyphenol microcapsules, stir at 500 rpm for 30 min, homogenize twice at 30 MPa, degas at -0.08 MPa for 15 min, and sterilize at 125℃ for 10 s to obtain an antioxidant liver-soothing, qi-regulating and depression-relieving composition containing bitter orange flower.

[0042] Example 3 An antioxidant composition containing bitter orange flower that soothes the liver, regulates qi, and relieves depression comprises the following raw materials in parts by weight: 35 parts bitter orange flower extract, 8 parts aromatic polyphenol microcapsules, 12 parts rose petals, 15 parts roasted jujube seed, 10 parts Hangzhou white chrysanthemum, 5 parts bergamot, 15 parts resistant dextrin, 8 parts fructooligosaccharides, 2 parts compound additives, and 40 parts deionized water.

[0043] The specific preparation method of the bitter orange flower extract is as follows: The bitter orange flowers were pulverized and passed through an 80-mesh sieve to obtain bitter orange pollen. The pollen was added to deionized water at a mass ratio of 1:15, and stirred at 400 rpm for 25 minutes at room temperature. The pH was adjusted to 5.5 with 0.3 mol / L citric acid solution. A complex enzyme (composed of cellulase with 30,000 U / g activity, pectinase with 50,000 U / g activity, and β-glucosidase with 20,000 U / g activity in a mass ratio of 3:3:1) was added at 1.2% of the bitter orange flower mass, and enzymatic hydrolysis was performed at 50℃ for 3 hours. After hydrolysis, the temperature was raised to 95℃ for 15 minutes to inactivate the enzyme, and then cooled to 37℃. The inoculum was then prepared at a rate of 10... 8 CFU / g of plant lactic acid bacteria were inoculated and fermented at 37℃ for 24h. After fermentation, the mixture was filtered through a 200-mesh nylon filter cloth, and the filtrate was collected. The filtrate was concentrated at 70℃ and -0.07MPa reduced pressure until the solid content was 25%. Then, it was spray-dried at an inlet air temperature of 180℃ and an outlet air temperature of 90℃ until the water content was 5% to obtain the bitter orange flower extract.

[0044] The specific preparation method of aromatic polyphenol microcapsules is as follows: The bitter orange blossom, Buddha's hand citron, and rose petals were mixed at a mass ratio of 6:3:2, pulverized, and passed through an 80-mesh sieve to obtain a mixed powder. The mixed powder was then added to deionized water at a mass ratio of 1:15 and extracted using ultrasound at 50℃ and 500W for 40 minutes. The extract was then filtered through a 200-mesh nylon filter cloth to obtain an aromatic polyphenol extract. This extract was placed in a concentration tank equipped with a condenser recovery device and concentrated under reduced pressure at 80℃ and -0.09MPa to 35% of its original volume to obtain a concentrated aromatic polyphenol extract. The final product was prepared by mixing bitter orange blossom, Buddha's hand citron, and rose petals at a mass ratio of 30... 1. Add composite wall material to the aromatic polyphenol concentrate, stir at 400 rpm for 25 min, adjust the pH to 5.5 with 0.3 mol / L citric acid solution, add 5% calcium chloride solution dropwise at a mass ratio of calcium chloride solution to aromatic polyphenol concentrate of 1:30, stir and gel at 40℃ and 500 rpm for 60 min, homogenize three times at 40 MPa, and then spray dry at an inlet air temperature of 180℃ and an outlet air temperature of 90℃ until the water content is 5% to obtain aromatic polyphenol microcapsules.

[0045] The compound additive consists of mogroside, erythritol and citric acid in a mass ratio of 1:15:1.

[0046] A preparation process for an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom includes the following preparation steps: S1. Weigh out rose petals, roasted jujube seeds, Hangzhou white chrysanthemum and Buddha's hand according to the weight parts, grind them separately to 80 mesh and mix them, add 50wt% deionized water, extract at 90℃ for 2h, filter with 200 mesh nylon filter cloth, collect the extract and concentrate at 70℃ and -0.08MPa to the solid content of 20% to obtain compound plant concentrate. S2. Heat 50wt% deionized water to 50℃, then add compound plant concentrate, resistant dextrin, oligofructose and compound additives, and stir at 600rpm for 40min to obtain mixed base liquid. S3. Cool the mixed base liquid to 35°C, add bitter orange flower extract and aromatic polyphenol microcapsules, stir at 600 rpm for 40 min, homogenize three times at 40 MPa, degas at -0.09 MPa for 20 min, and sterilize at 135°C for 15 s to obtain an antioxidant liver-soothing, qi-regulating and depression-relieving composition containing bitter orange flower.

[0047] Comparative Example 1 This comparative example provides an antioxidant liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom and its preparation process. The only difference from Example 2 is that the bitter orange blossom extract is replaced with an equal amount of unfermented bitter orange blossom extract; the other raw material types, amounts, and preparation process parameters are completely consistent with Example 2.

[0048] The specific preparation method of unfermented bitter orange flower extract is as follows: The bitter orange flowers were pulverized and passed through a 60-mesh sieve to obtain bitter orange pollen. The pollen was added to deionized water at a mass ratio of 1:12, and stirred at 300 rpm for 20 minutes at room temperature. The pH was adjusted to 5.0 with 0.2 mol / L citric acid solution. Then, a complex enzyme (consisting of cellulase with an activity of 25000 U / g, pectinase with an activity of 40000 U / g, and β-carotene with an activity of 15000 U / g) was added at 1.0% of the bitter orange flower mass. - Glucosidase was prepared in a mass ratio of 2:2:0.5 and enzymatically hydrolyzed at 45℃ for 2.5h. After enzymatic hydrolysis, the temperature was raised to 90℃ to inactivate the enzyme for 10min, cooled to 35℃, filtered through a 150-mesh nylon filter cloth, and the filtrate was collected. The filtrate was concentrated under reduced pressure of -0.06MPa at 60℃ to a solid content of 20%, and then spray-dried at an inlet air temperature of 170℃ and an outlet air temperature of 80℃ to a moisture content of 5% to obtain unfermented bitter orange flower extract.

[0049] Comparative Example 2 This comparative example provides an antioxidant liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom and its preparation process. The only difference from Example 2 is that the bitter orange blossom extract is replaced with an equal mass of unenzymatically hydrolyzed bitter orange blossom extract; the other raw material types, amounts, and preparation process parameters are completely consistent with Example 2.

[0050] The specific preparation method of unenzymatically hydrolyzed bitter orange flower extract is as follows: The bitter orange flowers were pulverized and passed through a 60-mesh sieve to obtain bitter orange pollen. The pollen was added to deionized water at a mass ratio of 1:12, and stirred at 300 rpm for 20 minutes at room temperature. The pH was adjusted to 5.0 with 0.2 mol / L citric acid solution. The inoculum size was 10... 7 CFU / g of plant lactic acid bacteria were inoculated and fermented at 35℃ for 20h. After fermentation, the mixture was filtered through a 150-mesh nylon filter cloth, and the filtrate was collected. The filtrate was concentrated under reduced pressure at 60℃ and -0.06MPa to a solid content of 20%, and then spray-dried at an inlet air temperature of 170℃ and an outlet air temperature of 80℃ to a moisture content of 5%, yielding unenzymatically hydrolyzed bitter orange extract.

[0051] Comparative Example 3 This comparative example provides an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom and its preparation process. The only difference from Example 2 is that the aromatic polyphenol microcapsules are replaced with an equal mass of simple mixed aromatic polyphenols; the other raw material types, amounts, and preparation process parameters are completely consistent with Example 2.

[0052] The specific preparation method of simple mixed aromatic polyphenols is as follows: The bitter orange blossom, Buddha's hand citron, and rose were mixed in a mass ratio of 5:2:1.5, pulverized, and passed through a 60-mesh sieve to obtain a mixed powder. The mixed powder was added to deionized water in a mass ratio of 1:12 and extracted by ultrasonication at 45℃ and 400W for 30 minutes. The extract was then filtered through a 150-mesh nylon filter cloth to obtain an aromatic polyphenol extract. The aromatic polyphenol extract was placed in a concentration tank equipped with a condenser recovery device and concentrated under reduced pressure at 70℃ and -0.08MPa to 30% of its original volume to obtain an aromatic polyphenol concentrate. The aromatic polyphenol concentrate was stirred at 300rpm for 20 minutes at room temperature, and the pH was adjusted to 5.0 with 0.2mol / L citric acid solution. The mixture was then stirred at 30℃ and 400rpm for 50 minutes. After homogenization twice at 30MPa, the mixture was spray-dried at an inlet air temperature of 170℃ and an outlet air temperature of 80℃ until the moisture content was 5% to obtain a simple mixed aromatic polyphenol.

[0053] Comparative Example 4 This comparative example provides an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom and its preparation process. The only difference from Example 2 is that the aromatic polyphenol microcapsules are replaced by a single-walled material to encapsulate the aromatic polyphenol complex by the same mass. The other raw material types, amounts, and preparation process parameters are completely consistent with Example 2.

[0054] The specific preparation method for single-walled aromatic polyphenol complexes is as follows: The bitter orange blossom, Buddha's hand citron, and rose petals were mixed at a mass ratio of 5:2:1.5, pulverized, and passed through a 60-mesh sieve to obtain a mixed powder. The mixed powder was then added to deionized water at a mass ratio of 1:12 and ultrasonically extracted at 45℃ and 400W for 30 minutes. The extract was filtered through a 150-mesh nylon filter cloth to obtain an aromatic polyphenol extract. This extract was placed in a concentration tank equipped with a condenser recovery device and concentrated under reduced pressure at 70℃ and -0.08MPa to 30% of its original volume to obtain a concentrated aromatic polyphenol extract. Finally, at a mass ratio of 25:1… Sodium alginate was added to the concentrated aromatic polyphenol solution, and the mixture was stirred at 300 rpm for 20 min at room temperature. The pH was adjusted to 5.0 with 0.2 mol / L citric acid solution. A 4% calcium chloride solution was added dropwise at a mass ratio of 1:25 to the concentrated aromatic polyphenol solution. The mixture was stirred at 30℃ and 400 rpm for 50 min to gel. After homogenization twice at 30 MPa, the mixture was spray-dried at an inlet air temperature of 170℃ and an outlet air temperature of 80℃ until the water content was 5%, thus obtaining a single-wall material-embedded aromatic polyphenol complex.

[0055] Comparative Example 5 This comparative example provides an antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom and its preparation process. The only difference from Example 2 is that the aromatic polyphenol microcapsules are replaced with single bitter orange blossom aromatic polyphenol microcapsules of equal mass, that is, the aromatic polyphenol microcapsules do not contain bergamot and rose, and only bitter orange blossom is used as raw material; the other types of raw materials, dosages, and preparation process parameters are completely consistent with Example 2.

[0056] The specific preparation method of single-generation flower aromatic polyphenol microcapsules is as follows: The bitter orange flower was pulverized and passed through a 60-mesh sieve to obtain single-flower bitter orange powder. The powder was added to deionized water at a mass ratio of 1:12, and ultrasonically extracted at 45℃ and 400W for 30 minutes. The extract was then filtered through a 150-mesh nylon filter cloth to obtain an aromatic polyphenol extract. This extract was placed in a concentration tank equipped with a condenser recovery device and concentrated under reduced pressure at 70℃ and -0.08MPa to 30% of its original volume to obtain a concentrated aromatic polyphenol solution. A composite wall material (made from a mass ratio of 1.5:1) was added to the concentrated aromatic polyphenol solution at a mass ratio of 25:1. Composed of sodium alginate, chitosan oligosaccharide and gum arabic in a ratio of 0.8:2, the mixture was stirred at 300 rpm for 20 min at room temperature. The pH was adjusted to 5.0 with 0.2 mol / L citric acid solution. A 4% calcium chloride solution was added dropwise at a mass ratio of 1:25 to the concentrated aromatic polyphenol solution. The mixture was stirred at 30℃ and 400 rpm for 50 min to gel. After homogenization twice at 30 MPa, the mixture was spray-dried at an inlet air temperature of 170℃ and an outlet air temperature of 80℃ until the moisture content was 5%, thus obtaining single-generation flower aromatic polyphenol microcapsules.

[0057] Performance testing To verify the technical effects of the antioxidant liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange flower in this invention, samples of the antioxidant liver-soothing, qi-regulating, and depression-relieving compositions containing bitter orange flower prepared in Examples 1-3 and Comparative Examples 1-5 were selected for the following performance tests.

[0058] 1. Testing Items and Methods (1) Determination of total flavonoid content The total flavonoid content in the sample was determined using the sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method. Rutin was used as a standard, and the absorbance was measured at 510 nm. A standard curve was plotted, and the total flavonoid content in the sample was calculated. The results are expressed as mg RE·g. -1 (Rutin equivalents / gram sample) is expressed as (Rutin equivalents / gram sample).

[0059] (2) Determination of DPPH free radical scavenging rate Prepare a solution of the sample to be tested with a mass concentration of 1.0 mg / mL. Mix 2 mL of the sample solution with 2 mL of 0.1 mmol / L DPPH ethanol solution, react in the dark for 30 min, and then measure the absorbance at 517 nm. Calculate the DPPH free radical scavenging rate according to the following formula: DPPH free radical scavenging rate / % = (A0-A1) / A0 × 100%; Where A0 is the absorbance of the blank control group (with deionized water replacing the sample), and A1 is the absorbance of the sample group.

[0060] (3) Determination of the 30-day retention rate of aromatic components Each group of samples was sealed and stored at 37℃ in the dark for 30 days. The total peak area of ​​the main volatile aromatic components, including limonene, linalool, nerol, citronellol, and phenylethyl alcohol, was determined by headspace solid-phase microextraction-gas chromatography-mass spectrometry. The total peak area of ​​the main volatile aromatic components in the sample before storage was A0, and the total peak area of ​​the main volatile aromatic components in the sample after 30 days of storage was A1. The retention rate of the aromatic components after 30 days was calculated according to the following formula: The 30-day retention rate of aromatic components is calculated as follows: (A1 / A0) × 100%.

[0061] (4) Determination of sedimentation rate Reconstitute each group of samples at a ratio of 1g composition to 10mL deionized water. Stir at 300rpm for 5min at room temperature to ensure complete dispersion and dissolution, preparing a reconstituted solution with a mass concentration of 0.1g / mL. Seal the reconstituted solution and store it in the dark at 37℃ for 30 days. After storage, thoroughly shake the sample, accurately weigh 10g of sample into a centrifuge tube, centrifuge at 4000rpm for 15min, discard the supernatant, and dry the precipitate at 105℃ to constant weight. Calculate the precipitation rate using the following formula: Sedimentation rate / % = Dry weight of sediment / Mass of sample weighed before centrifugation × 100%.

[0062] 2. Statistical processing methods All experiments were performed in triplicate, and results are expressed as mean ± standard deviation. One-way ANOVA was used for comparisons between groups, and a p-value < 0.05 was considered statistically significant.

[0063] 3. Test Results The specific test results are shown in Table 1.

[0064] Table 1. Performance test results of the antioxidant, liver-soothing, qi-regulating, and mood-relieving composition containing bitter orange flower. ; 4. Results Analysis As shown in Table 1, the antioxidant, liver-soothing, qi-regulating, and depression-relieving compositions containing bitter orange blossom prepared in Examples 1-3 are superior to those in Comparative Examples 1-5 in terms of total flavonoid content, DPPH free radical scavenging rate, and 30-day retention rate of aromatic components, and also have a lower precipitation rate. This indicates that the compositions prepared in this invention have better effective ingredient release, antioxidant properties, aromatic component retention, and system stability. Among them, Example 2 exhibits the best overall performance.

[0065] Compared with Example 2, Comparative Example 1, which replaced the bitter orange flower extract with unfermented bitter orange flower extract, showed a decrease in total flavonoid content and antioxidant properties, indicating that plant lactic acid bacteria fermentation helps to further promote the release and transformation of active ingredients in bitter orange flower, thereby improving the functional performance of the composition.

[0066] Compared with Example 2, Comparative Example 2, which replaced the bitter orange extract with unhydrolyzed bitter orange extract, showed a more significant decrease in the content of active ingredients and antioxidant properties. This indicates that enzymatic hydrolysis by compound enzymes can destroy the cell wall structure of bitter orange and promote the dissolution of active ingredients such as flavonoids, which is an important process for improving the utilization rate of the active ingredients of bitter orange.

[0067] Compared with Example 2, Comparative Example 3 replaced the aromatic polyphenol microcapsules with a simple mixed aromatic polyphenol, and its aromatic component retention effect and system stability were significantly reduced. This indicates that without composite wall material embedding and calcium ion gelation treatment, volatile aromatic components are more easily lost during storage, and the system is more prone to precipitation.

[0068] Compared with Example 2, Comparative Example 4, which uses a single wall material to encapsulate an aromatic polyphenol complex instead of aromatic polyphenol microcapsules, showed a significant decrease in the retention effect and stability of aromatic components. This indicates that the composite wall material composed of sodium alginate, oligochitosan, and gum arabic has better encapsulation protection and dispersion stabilization effects than the single wall material.

[0069] Compared with Example 2, Comparative Example 5 used single-flower bitter orange aromatic polyphenol microcapsules instead of aromatic polyphenol microcapsules prepared from bitter orange, bergamot and rose. Its antioxidant properties and aromatic component retention effect decreased, indicating that the combination of bitter orange, bergamot and rose can enrich the composition of active ingredients and aromatic components and enhance the overall performance of the composition.

[0070] This specific embodiment is merely an explanation of the present invention and is not intended to limit the invention. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A composition containing bitter orange blossom (Citrus aurantium) that has antioxidant, liver-soothing, qi-regulating, and depression-relieving properties, characterized in that, The ingredients include the following parts by weight: 25-35 parts bitter orange flower extract, 4-8 parts aromatic polyphenol microcapsules, 8-12 parts rose petals, 10-15 parts roasted jujube seed, 5-10 parts Hangzhou white chrysanthemum, 3-5 parts Buddha's hand, 10-15 parts resistant dextrin, 4-8 parts fructooligosaccharides, 1-2 parts compound additives, and 20-40 parts deionized water.

2. The antioxidant, liver-soothing, qi-regulating, and mood-relieving composition containing bitter orange blossom according to claim 1, characterized in that, The preparation method of the bitter orange flower extract is as follows: The bitter orange flower was crushed and sieved to obtain bitter orange pollen. The bitter orange pollen was added to deionized water, stirred evenly, the pH was adjusted, and a compound enzyme was added for enzymatic hydrolysis. After the enzymatic hydrolysis was completed, the enzyme was inactivated. After cooling, plant lactic acid bacteria were inoculated for fermentation. After the fermentation was completed, the mixture was filtered, the filtrate was collected and concentrated, and then spray-dried to obtain bitter orange flower extract.

3. The antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom according to claim 2, characterized in that, The compound enzyme is composed of cellulase with an enzyme activity of 20,000-30,000 U / g, pectinase with an enzyme activity of 30,000-50,000 U / g, and β-glucosidase with an enzyme activity of 10,000-20,000 U / g in a mass ratio of 1-3:1-3:0.2-1; the amount of the compound enzyme added is 0.8-1.2% of the mass of the bitter orange flower.

4. The antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom according to claim 1, characterized in that, The method for preparing the aromatic polyphenol microcapsules is as follows: The bitter orange blossom, Buddha's hand citron, and rose petals were mixed, pulverized, and sieved to obtain a mixed powder. The mixed powder was added to deionized water, ultrasonically extracted, and filtered to obtain an aromatic polyphenol extract. The aromatic polyphenol extract was placed in a concentration tank equipped with a condensation recovery device and concentrated under reduced pressure to obtain an aromatic polyphenol concentrate. A composite wall material was added to the aromatic polyphenol concentrate, stirred evenly, and the pH was adjusted. Then, calcium chloride solution was added dropwise for gelation. After homogenization and drying, aromatic polyphenol microcapsules were obtained.

5. The antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom according to claim 4, characterized in that, The composite wall material is composed of sodium alginate, oligochitosan and gum arabic in a mass ratio of 1-2:0.5-1:1-3.

6. The antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom according to claim 1, characterized in that, The composite additive is composed of mogroside, erythritol and citric acid in a mass ratio of 1:5-15:0.2-1.

7. A preparation process of the antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing *Citrus aurantium* as described in any one of claims 1-6, characterized in that, The preparation steps include the following: S1. Weigh out rose petals, roasted jujube seeds, Hangzhou white chrysanthemum and Buddha's hand according to the weight parts, crush them separately and mix them, add deionized water for extraction, filter, collect the extract and concentrate to obtain compound plant concentrate. S2. Heat the deionized water, then add the compound plant concentrate, resistant dextrin, fructooligosaccharides and compound additives, and stir evenly to obtain the mixed base liquid. S3. Cool the mixed base liquid, add bitter orange extract and aromatic polyphenol microcapsules, stir evenly, homogenize, degas under vacuum and sterilize to obtain an antioxidant liver-soothing and qi-regulating composition containing bitter orange.

8. The preparation process of the antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom according to claim 7, characterized in that, In step S1, the extraction temperature is 70-90℃, the extraction time is 1-2 hours, and the extraction is performed 1-2 times.

9. The preparation process of the antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange blossom according to claim 7, characterized in that, In step S3, the homogenization pressure is 20-40 MPa, and the homogenization is performed 1-3 times; the vacuum degree of the vacuum degassing is -0.07 MPa to -0.09 MPa, and the vacuum degassing time is 10-20 min.

10. The use of the antioxidant, liver-soothing, qi-regulating, and depression-relieving composition containing bitter orange as described in any one of claims 1-6 in the preparation of health foods, special dietary foods, or pharmaceutical compositions related to antioxidation, liver-soothing, mood easing, and stress improvement.