A malus hallings raspberry probiotic composition with paracasei lp36 as the core

Abstract

The application discloses a roxburgh rose probiotic composition taking paracasei LP36 as a core, which is composed of a composite probiotic powder, a bowel moistening and defecation planting component and a composite prebiotic, LP36 (CCTCC M 20232287) is a core bacterium, and edible probiotics are supplemented; the planting component contains roxburgh rose powder, hemp seeds, round achenes of plantago, momordica grosvenori and cassia seed. LP36 produces short-chain fatty acids to activate 5-HT to promote intestinal motility, roxburgh rose dietary fiber absorbs water to swell and has antioxidant properties, the planting component moistens dryness and lubricates the intestines, the prebiotic promotes bacterial proliferation, and four synergies improve constipation. Animal experiments show that the composition significantly shortens the time of the first black stool, increases the number of stool particles, improves the propulsion rate and the water content of feces, and up-regulates Muc2 and SCFAs, and the effect is better than that of single bacteria, the planting component and lactulose; the composition is full food grade, has no stimulation, and can be made into a solid beverage, a tablet or a capsule.

Description

Technical Field

[0001] This invention belongs to the field of food and medicine homology microecological intestinal health food technology, specifically involving a composition with Lactobacillus paracasei LP36 as the essential core strain, combined with edible auxiliary probiotics, standardized freeze-dried prickly pear powder, various intestinal lubricating and laxative plant extracts and compound prebiotics, as well as its preparation method and application; the composition can be used to prepare oral preparations such as solid beverages, compressed candies, granules, and hard capsules for preventing, improving, and relieving slow transit and intestinal dryness type functional constipation, increasing fecal water content, accelerating intestinal peristalsis, repairing colonic mucosa, and regulating intestinal flora balance. Background Technology

[0002] Functional constipation (FC) is one of the most common functional gastrointestinal disorders in clinical practice, mainly characterized by reduced bowel movement frequency, hard stools, straining during defecation, a feeling of incomplete evacuation, and delayed intestinal transit. Epidemiological studies show that the prevalence of functional constipation in Chinese adults is approximately 8.5%–10.9%, with a higher prevalence in women than men, and it increases significantly with age, reaching over 16% in people aged 65 and above. Long-term constipation not only affects quality of life but is also associated with gut microbiota dysbiosis, decreased intestinal mucosal barrier function, local low-grade inflammation, and mood disorders such as anxiety and depression, thus increasing the disease burden.

[0003] From a pathological perspective, the core causes of functional constipation can be divided into three layers: ① Intestinal flora imbalance, decreased abundance of beneficial bacteria that produce short-chain fatty acids (SCFAs), and insufficient intestinal motility; ② Reduced mucus secretion by goblet cells in the colonic mucosa, leading to intestinal dehydration and dryness; ③ Insufficient dietary fiber intake, insufficient secretion of motility-related serotonin (5-HT) and SCFAs, resulting in small stool volume that is difficult to stimulate intestinal emptying.

[0004] Currently, laxative products on the market have the following significant drawbacks: Simple laxatives (such as senna leaves and rhubarb, which are stimulant laxatives) are highly irritating, and long-term use can cause melanosis coli and drug dependence, with constipation worsening after discontinuation; Single laxative plant extracts (such as hemp seed and cassia seed used alone) only mildly lubricate the intestines, cannot regulate the intestinal flora, have a slow onset of action, and lack the fundamental effect of improving the flora; Ordinary probiotic formulas lack a targeted laxative core strain, and the strains have a weak ability to produce short-chain fatty acids, making it difficult to stimulate enterochromaffin cells to secrete 5-HT to improve intestinal motility, and the laxative effect of probiotics is highly strain-specific; Existing "bacteria + fruit and vegetable compound" patents all fix all auxiliary probiotics, and competitors can circumvent the patents by simply replacing one auxiliary strain, resulting in weak protection barriers; In addition, there is a lack of multi-target synergistic laxative systems, and single raw materials only act on a single target, resulting in limited comprehensive laxative effects.

[0005] Existing research confirms that *Lactobacillus paracasei*, after colonizing the colon, can produce large amounts of short-chain fatty acids such as acetic acid and propionic acid, activate enterochromaffin cells to release 5-HT, promote the proliferation of Cajal interstitial cells, and accelerate colonic transit; simultaneously, it upregulates colonic Muc2 mucin expression, increases fecal water content, and improves constipation from three aspects: flora, motility, and mucosa. (Prickly pear) Rosa roxburghii Tratt This fruit, a specialty of Southwest my country, is rich in highly active soluble dietary fiber, polyphenols, vitamin C, and superoxide dismutase (SOD). Its dietary fiber has a strong water-absorbing and swelling capacity, increasing stool volume and softening hard stools. Polyphenols and vitamin C can scavenge free radicals, protect probiotics from stomach acid and bile, and improve the survival rate of live intestinal bacteria. Prickly pear polysaccharides also have clear natural prebiotic activity, promoting the proliferation of lactic acid bacteria and bifidobacteria. Hemp seeds, psyllium husk, monk fruit, and cassia seeds are classic ingredients for laxatives: hemp seeds are rich in oils, moisturizing and lubricating the intestines; psyllium husk is rich in dietary fiber, absorbing water and swelling; monk fruit polysaccharides moisturize the intestinal wall; cassia seeds gently promote intestinal emptying. The combined use of these plant extracts is gentle, non-irritating, and non-addictive, suitable for long-term constipation management.

[0006] Lactobacillus paracasei LP36 ( Lacticaseibacillus paracasei LP36 is a functional probiotic independently screened by the applicant. It has characteristics such as acid resistance, bile salt resistance, and good intestinal adaptability. It has been deposited at the China Center for Type Culture Collection, with accession number CCTCC NO: M 20232287.

[0007] According to the search, there is currently no prior technology that uses Lactobacillus paracasei LP36 as the only essential core strain for laxative purposes, removes restrictions on the types of other auxiliary probiotics, and combines it with prickly pear and other mild laxative ingredients to construct a multi-target laxative composition. This technology is still in its infancy. Summary of the Invention

[0008] In view of the shortcomings of existing laxative products, such as easy dependence, single target, and easy replacement of patented formulas by strains, the purpose of this invention is to provide a prickly pear laxative probiotic composition with Lactobacillus paracasei LP36 as an indispensable core strain and auxiliary probiotics of any species.

[0009] Another object of the present invention is to provide a method for preparing the above-described composition.

[0010] Another object of the present invention is to provide the use of the above composition in the preparation of functional foods, dietary supplements and probiotics for relieving functional constipation.

[0011] To achieve the above objectives, the present invention adopts the following technical solution: A prickly pear probiotic composition with Lactobacillus paracasei LP36 as its core, designed to relieve functional constipation, comprises the following components by weight: A. 10-18 parts of compound probiotic powder, composed of 3-7 parts of *Lactobacillus paracasei* LP36 powder and 7-11 parts of auxiliary probiotic powder; LP36 is an essential core strain, with preservation number CCTCC NO: M 20232287; the auxiliary probiotics are any one or more probiotics permitted for consumption by the state, selected from *Lactobacillus*, *Bifidobacterium*, *Streptococcus*, *Pediococcus*, and *Lactococcus*; the total live bacteria content of the compound probiotic powder is ≥ / 100 parts of the composition.

[0012] B. The laxative and bowel-regulating plant extract active ingredients, consisting of 14-30 parts, are composed of 9-16 parts of low-temperature freeze-dried prickly pear powder, 2-5 parts of hemp seed powder, 2-4 parts of psyllium husk powder, 1-3 parts of monk fruit powder, and 0-2 parts of cassia seed powder; the low-temperature freeze-dried prickly pear powder has a total polyphenol content ≥3.5wt%, SOD activity ≥8000U / g, and soluble dietary fiber content ≥12wt%; all raw materials are food-grade mild laxative ingredients.

[0013] C. 52-76 parts of a complex prebiotic carrier, composed of 22-30 parts of resistant dextrin, 10-15 parts of fructooligosaccharides, 11-13 parts of isomaltooligosaccharides, 6-9 parts of inulin, and 3-9 parts of galactooligosaccharides.

[0014] The four-fold synergistic laxative mechanism of the composition of the present invention is as follows: (1) The LP36 core strain secretes a large amount of SCFAs, upregulates 5-HT, repairs colonic goblet cells, and enhances intestinal peristalsis from the root of the flora; (2) Freeze-dried prickly pear highly active soluble dietary fiber absorbs water and expands, softens dry and hard stools, and provides antioxidant protection for the survival of all probiotics in the intestines; (3) The compound plant extracts of hemp seed, psyllium husk, monk fruit, and cassia seed gently moisten and lubricate the intestines, increase intestinal mucus secretion, and have no irritating laxative side effects; (4) The compound prebiotics continuously supply nutrients to probiotics, promote the continuous proliferation of beneficial bacteria, maintain the normal bowel rhythm of the intestines for a long time, and prevent constipation from recurring.

[0015] The present invention also provides a method for preparing the composition, comprising: anaerobic fermentation of the strains, low-temperature centrifugation, and vacuum freeze-drying to obtain single-strain powder, or direct low-temperature spray drying after single-strain fermentation to obtain units, followed by low-temperature mixing of the single-strain powder; sieving and drying of plant extract raw materials; premixing of prebiotics; low-temperature mixing at controlled temperature of 25-35°C; and finally packaging or molding into dosage forms such as solid beverages, compressed candies, granules, hard capsules, and oral powders.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. Unique patent barrier, high difficulty in circumvention: Only LP36 is limited to the essential laxative core strain in the formula, while the other auxiliary probiotics are completely open and unrestricted in species; no matter whether competitors replace or add or remove any auxiliary probiotics, as long as they contain LP36, prickly pear and intestinal-moistening plant extracts, and compound prebiotics, they will fall within the protection scope of this invention, which solves the shortcoming of traditional fixed multi-strain formulas that are easy to replace and circumvent.

[0017] 2. LP36, combined with prickly pear and a complex intestinal-moistening plant extract, forms a four-fold synergistic laxative system: LP36 produces SCFAs that activate the 5-HT pathway, enhancing intestinal motility and addressing the root cause of constipation due to insufficient motility; prickly pear's soluble dietary fiber absorbs water and swells, while psyllium husk increases stool volume, physically stimulating intestinal emptying; hemp seed, monk fruit, and cassia seed's multiple oils and polysaccharides moisturize the intestinal wall, increasing stool water content and improving dryness and hardness; prickly pear polyphenols and vitamin C scavenge free radicals in the colon, protecting LP36 and all auxiliary probiotics from stomach acid and bile, increasing colonic colonization rate, and providing long-term regulation of the gut microbiota.

[0018] 3. Animal experiments verify significant laxative effect: In a mouse model of constipation, the time to excretion of the first black stool in the low- and high-dose groups of the composition of this invention was shortened by 57.7% and 66.9% respectively compared with the model group. The small intestinal ink propulsion rate was increased by 1.28 times and 1.58 times respectively. The fecal water content increased from 41.3% to 62.8% and 67.4%. The number of fecal particles in 6 hours increased from 3.2 to 10.3 and 11.9. The relative expression of colonic Muc2 mucin recovered from 36.2% to 79.5% and 92.8%. The total SCFAs content in the colon increased from 3.15 μmol / g to 7.94 μmol / g and 9.36 μmol / g. All indicators were superior to those of the constipation model group, LP36 single bacterial powder group, pure prickly pear plant extract group and lactulose positive control group.

[0019] 4. Multi-target gentle laxative, non-irritating and non-addictive: It abandons stimulating laxatives such as rhubarb and senna leaves, and uses only food-grade gentle laxative ingredients. Combined with probiotics to regulate the gut microbiota from the root, it softens stool in the short term and restores normal bowel rhythm in the long term. It is suitable for long-term consumption by the elderly, postpartum women, and people who sit for long periods of time.

[0020] 5. Wide industrial applicability: The auxiliary probiotics can be freely combined according to the market, cost and population. One patent covers multiple derivative laxative products; the raw materials of the formula are readily available, the low-temperature production process is simple, the live bacteria have high stability, and the commercialization value is high. Attached Figure Description

[0021] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings: Figure 1 The effect of the composition of the present invention on the defecation function of a mouse model of constipation induced by loperamide is shown in the figure, where (a) is the time of first black stool excretion, (b) is the number of fecal pellets in 6 hours, (c) is the fecal water content, and (d) is the small intestinal ink propulsion rate.

[0022] Figure 2 The effects of the composition of the present invention on colonic mucosal repair and intestinal motility substances in constipated mouse models are shown in (a) for the relative expression level of colonic Muc2 mucin and (b) for the total short-chain fatty acid content in the colon. Detailed Implementation

[0023] The following drawings disclose several embodiments of the present invention. For clarity, many practical details will be described in the following description. However, it should be understood that these practical details are not intended to limit the invention. That is, in some embodiments of the invention, these practical details are not essential. Furthermore, for the sake of simplicity, some conventional structures and components will be shown in the drawings in a simple schematic manner.

[0024] Example 1: Preparation of compound probiotic powder Lactobacillus paracasei LP36 (CCTCC NO: M 20232287) was inoculated into MRS liquid medium and anaerobically cultured at 37°C for 18–24 h. The fermentation broth was centrifuged at 4°C and 6000 r / min for 15 min to collect the bacterial cells. A freeze-drying protectant (based on the weight of the bacterial sludge, 5%–10% trehalose, 3%–8% skim milk powder, and 5%–15% maltodextrin) was added, and the mixture was then spray-dried at low temperature or freeze-dried under vacuum to obtain LP36 single-strain bacterial powder with a viable count ≥ / g. For example, *Lactobacillus plantarum*, *Lactobacillus rhamnosus*, *Bifidobacterium animalis* subsp. *lactobacter*, and *Lactobacillus acidophilus* were selected as auxiliary probiotics and their individual bacterial powders were prepared using the same method. 3–7 parts by weight of LP36 bacterial powder and 7–11 parts by weight of auxiliary probiotic powder were weighed and mixed evenly under conditions of ≤25℃ and ≤40% relative humidity to obtain the compound probiotic powder; the entire operation temperature was ≤40℃.

[0025] Example 2: Preparation of a solid beverage made from a prickly pear probiotic composition Weigh out the following by weight: 13 parts of compound probiotic powder (including 5 parts of LP36 powder and 8 parts of auxiliary probiotic powder), 13 parts of low-temperature freeze-dried prickly pear powder, 3 parts of hemp seed powder, 3 parts of psyllium husk powder, 2 parts of monk fruit powder, 1 part of cassia seed powder, 28 parts of resistant dextrin, 12 parts of fructooligosaccharides, 12 parts of isomaltooligosaccharides, 8 parts of inulin, and 5 parts of galactooligosaccharides.

[0026] Preparation steps: (1) Pass the five plant extracts through a 100-mesh sieve and dry them with hot air at 40℃ until the moisture content is ≤4%; (2) Add the five prebiotics to a three-dimensional mixer and stir at low speed for 15 minutes; (3) Mix the prebiotic carrier with all the plant extracts evenly, control the material temperature at 25-35℃, add the compound probiotic powder, and continue mixing for 20-30 minutes; (4) Package into 1.5-5g / bag strips of solid beverage, seal with aluminum foil, and store in a cool, dark place. The total number of live probiotics per 100g of product is ≥ When consuming, mix with warm water below 40℃.

[0027] Example 3: Preparation of Prickly Pear Probiotic Compressed Candy Take the total powder of the composition obtained in Example 2, add 1% by weight of microcrystalline cellulose and 0.5% by weight of magnesium stearate, mix evenly and compress into tablets, each tablet weighing 0.6g, to obtain prickly pear probiotic compressed candy, packaged in aluminum-plastic blister packs.

[0028] Example 4: Preparation of Prickly Pear Probiotic Hard Capsules The total powder of the composition obtained in Example 2 was filled into hydroxypropyl methylcellulose empty capsules, 0.4g per capsule, to obtain prickly pear probiotic hard capsules; the number of LP36 live bacteria in each capsule is ≥1×10⁻⁶. 8 CFU.

[0029] Experimental Example 1: Verification of the laxative effect of the composition on a mouse model of constipation induced by loperamide 1. Materials and Methods. Six-week-old SPF-grade male KM mice were randomly divided into seven groups (n=12 per group) after acclimatization: ① Blank control group: administered saline by gavage; ② Constipation model group: modeling + saline; ③ LP36 single bacterial powder control group; ④ Pure prickly pear + intestinal lubricating plant extract control group (no probiotics); ⑤ Positive control group: lactulose oral solution 0.8 g / kg; ⑥ Low-dose group of the composition of this invention: 0.5 g / kg; ⑦ High-dose group of the composition of this invention: 2 g / kg. Each group received the corresponding sample by gavage daily for 14 consecutive days. One hour after the last administration, all mice except the blank group were administered loperamide hydrochloride 5 mg / kg by gavage to establish a slow-transit functional constipation model. After modeling, mice were administered ink by gavage, and the time of first black stool expulsion, number of fecal pellets at 6 hours, fecal water content, and small intestinal ink propulsion rate were recorded. At the end of the experiment, colon tissue was collected to detect the relative expression level of Muc2 mucin and the content of total short-chain fatty acids (SCFAs) in the colon.

[0030] 2. Experimental Results. The test results for each group are shown in Table 1. Figure 1 and Figure 2 .

[0031] Table 1. Effects of the composition on laxative efficacy in constipation model mice (mean, n=12)

[0032] Note: The data in the table are the average results of the tests on 12 mice in each group.

[0033] 3. Comprehensive Experimental Conclusions. Regardless of the type of compliant probiotic supplement used, the composition with LP36 as the core and prickly pear combined with multiple mild laxative ingredients exhibits significant synergistic laxative effects. Data from seven complete animal studies show that the low- and high-dose groups of this invention are superior to the constipation model group, the LP36 single-herb powder group, the pure prickly pear plant extract group, and the lactulose-positive control group in all laxative indicators, including intestinal transit speed, fecal particle number, fecal water content, small intestinal propulsion rate, colonic mucus secretion, and SCFAs generation. The formula is mild and non-irritating, and can effectively regulate the intestinal flora for a long time. Its novelty, inventiveness, and industrial applicability are outstanding.

[0034] Information on the preservation of biological materials The preservation information of the biomaterial Lactobacillus paracasei LP36 involved in this invention is as follows: Name of depositary institution: China Center for Type Culture Collection (CCTCC); Address of the depository: Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, China; Accession number: CCTCC NO:M 20232287; Deposit date: November 20, 2023; Classification and nomenclature: Lactobacillus paracasei ( Lacticaseibacillus paracasei LP36.

[0035] The above description is merely an embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the principles of the present invention should be included within the scope of the claims of the present invention.

[0036] It should also be noted that: (1) The auxiliary probiotics described in this invention are not limited to the strains exemplified in the examples, but include any probiotic strains in the "List of Strains that can be Used in Food" approved and published by the national health authorities and subsequent updates. For example, the new food raw material probiotics approved in the "Announcement on 24 'Three New Foods' including Lactobacillus curvature" (No. 4 of 2022) issued by the State on August 25, 2022, as well as the probiotic strains allowed to be used in ordinary foods in subsequent revisions and supplements, can all be used as auxiliary probiotics in this invention. As long as the formula contains Lactobacillus paracasei LP36 as an essential core strain, and is combined with the intestinal lubricating and laxative plant extract active ingredients and complex prebiotic carriers described in this invention, it falls within the protection scope of this invention.

[0037] (2) The resistant dextrin, fructooligosaccharides, isomaltooligosaccharides, inulin, and galactooligosaccharides listed in the compound prebiotic carrier of the present invention are merely exemplary preferred solutions and are not a limitation on the types of prebiotics. The compound prebiotic carrier of the present invention may also include any other prebiotic ingredients that have been approved by the national competent authority for use in ordinary foods or health foods, such as xylooligosaccharides, mannose, stachyose, raffinose, polydextrose, isomaltulose, lactulose, soybean oligosaccharides, chitosan oligosaccharides, yeast β-glucan, etc. As long as the prebiotic ingredient can selectively promote the proliferation of beneficial bacteria in the intestine and provide the nutrients required for the growth of LP36 and auxiliary probiotics, it can replace or partially replace the types of prebiotics listed in the present invention, and work synergistically with the compound probiotic powder and the laxative plant extract active ingredients of the present invention to exert a laxative effect.

Claims

1. A probiotic composition of prickly pear with Lactobacillus paracasei LP36 as its core, characterized in that, By weight, it consists of the following components: A. 10-18 parts of compound probiotic powder (freeze-dried or low-temperature spray-dried); the compound probiotic powder consists of 3-7 parts of *Lactobacillus paracasei* LP36 bacterial powder and 7-11 parts of auxiliary probiotic powder; *Lactobacillus paracasei* LP36 is an essential core strain, and its classification name is *Lactobacillus paracasei* (…). Lacticaseibacillus paracasei LP36 has been deposited at the China Center for Type Culture Collection, with accession number CCTCC NO: M 20232287; the auxiliary probiotics are any one or more probiotics listed in the list of strains that can be used in food published by the national competent authority. B. 14-30 parts of plant extract active ingredients for promoting bowel movement and relieving constipation, composed of 9-16 parts of low-temperature freeze-dried prickly pear powder, 2-5 parts of hemp seed powder, 2-4 parts of psyllium husk powder, 1-3 parts of monk fruit powder, and 0-2 parts of cassia seed powder; the low-temperature freeze-dried prickly pear powder has a total polyphenol content ≥3.5wt%, superoxide dismutase activity ≥8000U / g, and soluble dietary fiber content ≥12wt%. C. 52-76 parts of complex prebiotic carrier, composed of 22-30 parts of resistant dextrin, 10-15 parts of fructooligosaccharide, 11-13 parts of isomaltooligosaccharide, 6-9 parts of inulin and 3-9 parts of galactooligosaccharide; The total number of live probiotics per 100 parts by weight of the composition is ≥ .

2. The prickly pear probiotic composition according to claim 1, characterized in that, The probiotics are selected from Lactobacillus genus ( Lactobacillus Bifidobacterium spp. Bifidobacterium Streptococcus ( Streptococcus ), Pediococcus ( Pediococcus Lactococcus spp. Lactococcus Any one or more of the following.

3. The prickly pear probiotic composition according to claim 1, characterized in that, The viable count of the *Lactobacillus paracasei* LP36 powder is ≥ / g.

4. The prickly pear probiotic composition according to claim 1, characterized in that, By weight, it consists of the following components: 13 parts of compound probiotic powder, 13 parts of low-temperature freeze-dried prickly pear powder, 3 parts of hemp seed powder, 3 parts of psyllium husk powder, 2 parts of monk fruit powder, 1 part of cassia seed powder, 28 parts of resistant dextrin, 12 parts of fructooligosaccharide, 10 parts of isomaltooligosaccharide, 8 parts of inulin, and 7 parts of galactooligosaccharide; the compound probiotic powder is composed of 5 parts of Lactobacillus paracasei LP36 bacterial powder and a total of 8 parts of auxiliary probiotic powder.

5. The prickly pear probiotic composition according to any one of claims 1 to 4, characterized in that, The composition is prepared as any one of the following dosage forms: ready-to-eat solid beverage, compressed candy, granules, hard capsules, or oral powder.

6. A method for preparing the prickly pear probiotic composition according to any one of claims 1 to 5, characterized in that, Includes the following steps: (1) The Lactobacillus paracasei LP36 and the selected auxiliary probiotics were subjected to anaerobic liquid fermentation, low-temperature centrifugation, low-temperature spraying or vacuum freeze drying to obtain single bacterial powders. The single bacterial powders were then mixed at low temperature to obtain compound probiotic powders. The entire process was carried out at a temperature ≤40℃. (2) The low-temperature freeze-dried prickly pear powder, hemp seed powder, psyllium husk powder, monk fruit powder and cassia seed powder were passed through a 100-mesh sieve and dried in hot air at 40℃ until the moisture content was ≤4% to obtain the active plant extract components for lubricating the intestines and relieving constipation. (3) Add resistant dextrin, fructooligosaccharide, isomaltooligosaccharide, inulin and galactooligosaccharide to a mixer and stir and premix for 15 min to obtain a composite prebiotic carrier; (4) Premix the compound prebiotic carrier and the intestinal lubricating and laxative plant extract active ingredients evenly, control the material temperature at 25-35℃, add the compound probiotic powder, and continue mixing for 20-30 minutes to obtain the total powder of the composition; (5) Dispense or mold the total mixture of the composition into the required dosage form, and store it in a sealed container away from light.

7. The preparation method according to claim 6, characterized in that, Step (4) The temperature of the materials during the entire mixing process shall not exceed 35°C; Step (5) The molding process is to directly package the solid beverage into strips, or add microcrystalline cellulose and magnesium stearate to compress it into tablets, or fill it into hard capsules.

8. The use of the prickly pear probiotic composition according to any one of claims 1 to 5 in the preparation of functional foods that lubricate the intestines and promote bowel movements, shorten intestinal transit time, and improve slow-transit functional constipation.

9. The use of the prickly pear probiotic composition according to any one of claims 1 to 5 in the preparation of an intestinal conditioning dietary supplement that increases fecal water content, softens hard feces, and reduces the difficulty of defecation.

10. The use of the prickly pear probiotic composition according to any one of claims 1 to 5 in the preparation of a microecological preparation for repairing colonic mucosa, promoting the secretion of colonic Muc2 mucin, regulating the balance of intestinal flora, and preventing the recurrence of constipation.

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