A ternary oil composition with high-efficiency anti-wrinkle synergistic and self-emulsifying synergistic effects and application thereof

Abstract

The present application relates to a ternary oil composition with high-efficiency anti-wrinkle synergistic and self-emulsifying synergistic effect and application thereof. The present application adds Hongshan tea flower longshou fungus fermented oil, ginseng seed oil and ganoderma lucidum spore oil in the composition, and the three synergistically precisely resist the inflammatory damage and collagen degradation mechanism of skin aging from multiple pathways such as inflammation regulation, promotion of collagen neogenesis and reduction of collagen degradation, while promoting collagen neogenesis and repairing skin barrier, so as to significantly improve the anti-aging effect. Moreover, the amphiphilic fungal lipids in ganoderma lucidum spore oil and the liposoluble ginsenosides, phytosterols and polar fatty acid components in ginseng seed oil can form a synergistic effect with the interfacial active substances in Hongshan tea flower longshou fungus fermented oil, construct a composite interfacial film at the oil-water interface, improve the self-emulsifying ability of the oil system, solve the problem of poor self-emulsifying ability of the three plant oils themselves, so that no additional emulsifier needs to be introduced in cosmetics, and the production cost is reduced.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a ternary oil composition with highly efficient anti-wrinkle synergistic and self-emulsifying effects and its application. Background Technology

[0002] Current research on plant oils focuses on two main areas: either reducing transepidermal water loss through the basic moisturizing mechanisms of oils to achieve a hydrating and soothing effect, or scavenging free radicals and promoting cell activity through unsaturated fatty acids to achieve anti-oxidation and anti-aging effects. However, skin problems often involve more than one issue; barrier damage and aging almost always occur simultaneously, influencing each other and being closely related to skin condition.

[0003] For example, in patent application CN117653561A, although the anti-aging composition includes various plant oils such as camellia seed oil, ginseng seed oil, and Ganoderma lucidum spore oil, it only focuses on the synergistic penetration-enhancing effect of the unsaturated fatty acids in plant oils, neglecting their role in regulating collagen regeneration and collagen protection. Furthermore, ordinary camellia seed oil is mainly composed of triglycerides, with large lipid molecular weights and low transdermal efficiency, and requires the addition of emulsifiers in cosmetics as it lacks self-emulsifying ability. In patent application CN118217191A, various plant oils, including ginseng seed oil, are used as an anti-aging composition, emphasizing their ability to effectively eliminate free radicals, promote skin cell activity, and promote the production of large amounts of intercellular lipids. The aim is to fill the skin with these lipids, restoring smoothness and significantly smoothing wrinkles, but this ignores the important cause of wrinkles: reduced collagen production and biodegradation.

[0004] Therefore, how to improve the anti-aging effects of plant oils through multiple synergistic pathways while constructing a self-emulsifying system is a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a ternary oil composition with highly efficient anti-wrinkle synergistic and self-emulsifying effects, and its applications.

[0006] The objective of this invention can be achieved through the following methods: In a first aspect, the present invention provides a ternary oil composition with highly efficient anti-wrinkle synergistic and self-emulsifying effects, comprising the following components in parts by weight: 1-5 parts of Ganoderma lucidum spore oil, 1-5 parts of ginseng seed oil, 1-15 parts of red camellia longevity bacteria fermented oil, and 1-10 parts of antioxidant.

[0007] As one embodiment of the present invention, the total triterpenoid content in the Ganoderma lucidum spore oil is >30%.

[0008] As one embodiment of the present invention, the preparation method of the red camellia longevity bacteria fermented oil includes: inoculating activated spore yeast into a basic fermentation medium containing red camellia seed oil for fermentation culture to obtain fermentation broth; then sterilizing the fermentation broth and letting it stand, allowing it to naturally separate into layers, collecting the upper oil phase to obtain red camellia longevity bacteria fermented oil.

[0009] Furthermore, the basic fermentation medium comprises: glucose 4-6 g / L, peptone 18-22 g / L, yeast powder 9-11 g / L, potassium dihydrogen phosphate 3-4 g / L, dipotassium hydrogen phosphate 4-6 g / L, diammonium hydrogen phosphate 3-4 g / L, sodium chloride 4-6 g / L, and magnesium sulfate heptahydrate 0.8-1.2 g / L; the amount of camellia seed oil added is 100%-200% of the mass of the basic fermentation medium.

[0010] As one embodiment of the present invention, the relative content of diglycerides in the fermented oil of Camellia longevity bacteria is 30%-55%, and the content of carotenoids is 150-200ug / g.

[0011] As one embodiment of the present invention, the ginseng seed oil contains linolenic acid and linoleic acid, wherein the mass percentage of linolenic acid is 0.01-1.4% and the mass percentage of linoleic acid is 0.1-19.4%.

[0012] Specifically, the fermented oil of red camellia flower longevity bacteria has a high content of diglycerides and a fast metabolic rate, preventing it from accumulating in cells. This prevents skin inflammation caused by oil accumulation from the root. Together with ginsenosides in ginseng seed oil and ganoderic triterpenes in Ganoderma lucidum spore oil, the three cut off the triggering signal from the upstream of inflammation, directly clearing oxygen free radicals, reducing lipid and protein oxidation, and reducing collagen degradation caused by oxidative stress. Furthermore, red camellia seed oil and Ganoderma lucidum spore oil promote type I collagen synthesis by regulating gene and enzyme activity, inhibiting MMP expression, and upregulating COL1A1 gene expression in fibroblasts.

[0013] In one embodiment of the present invention, the antioxidant is selected from at least one of tocopherol and pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester. Tocopherol is preferred.

[0014] Secondly, the present invention provides a method for preparing the ternary oil composition, comprising the following steps: Step 1: Weigh out Ganoderma lucidum spore oil, ginseng seed oil, and red camellia longevity fungus fermented oil, stir well, and obtain the composition; Step 2: Add antioxidants and stir well to obtain the final product.

[0015] In one embodiment of the present invention, in step one or step two, the stirring temperature is 20-40℃ and the stirring speed is 100-300 rpm / min.

[0016] Thirdly, the present invention provides the use of the ternary oil composition in the preparation of cosmetics.

[0017] Fourthly, the present invention provides a cosmetic product containing 0.1 to 10 wt% of the ternary oil composition described above.

[0018] As one embodiment of the present invention, the cosmetic includes one of the following: serum, essence lotion, facial mask liquid, cream, sunscreen, makeup, and personal care products.

[0019] Compared with the prior art, the present invention has the following beneficial effects: 1. This invention adds red camellia longevity bacteria fermented oil, ginseng seed oil, and Ganoderma lucidum spore oil to the composition. The three work synergistically to precisely combat the inflammatory damage and collagen degradation mechanism of skin aging through multiple pathways, such as inflammation regulation, promoting collagen regeneration, and reducing collagen degradation. At the same time, it promotes collagen regeneration, repairs the skin barrier, and achieves a significant improvement in anti-aging efficacy.

[0020] 2. This invention utilizes the triterpenoids, ergosterol, and other amphiphilic fungal lipids contained in Ganoderma lucidum spore oil, and the fat-soluble saponins, phytosterols, and polar fatty acid components in ginseng seed oil. These components can synergistically interact with natural surfactants such as monoglycerides, diglycerides, and free fatty acids produced by microbial metabolism in Camellia sinensis fermented oil. Together, they are oriented and arranged at the oil-water interface to construct a dense and resilient composite interfacial film. This effectively reduces the interfacial tension between the oil and water phases, weakens the interfacial repulsion, and promotes the spontaneous dispersion of the oil system under mild conditions without strong external shearing or with only simple stirring to form a fine and uniform emulsion droplet structure. This significantly improves the self-emulsifying ability and stability of the oil system, solving the problem of poor self-emulsifying ability of the three plant oils themselves. This allows them to be used in cosmetics without the need for additional emulsifiers, reducing production costs.

[0021] 3. This invention uses red camellia longevity bacteria fermented oil. After microbial fermentation, the content of diglycerides increases while the content of triglycerides decreases, which significantly reduces the molecular weight of the red camellia longevity bacteria fermented oil, thereby improving its transdermal efficiency.

[0022] 4. This invention removes free radicals during the oxidation process by adding antioxidants, thereby delaying the oxidation and deterioration of oils and protecting the activity and stability of the oil-soluble composition.

[0023] 5. The oil composition of the present invention is applicable to a variety of cosmetic systems. The composition has good stability, the preparation process is simple, no special equipment is required, and it is easy to mass-produce. Attached Figure Description

[0024] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings: Figure 1 Examples 1-4 and Examples 1-3 are shown in the appearance pictures; Figure 2 Micrograph of the emulsion in Example 3; Figure 3 Micrograph of the emulsion in Comparative Example 1; Figure 4 Micrograph of the emulsion in Comparative Example 2; Figure 5 Micrograph of the emulsion in Comparative Example 3; Figure 6 Micrograph of the emulsion in Comparative Example 5. Detailed Implementation

[0025] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. The following examples are implemented under the premise of the technical solution of the present invention, providing detailed implementation methods and specific operating procedures, which will help those skilled in the art to further understand the present invention. It should be noted that the scope of protection of the present invention is not limited to the following embodiments; any adjustments and improvements made under the concept of the present invention are all within the scope of protection of the present invention.

[0026] Raw materials used in the embodiments and comparative examples of this invention: Ganoderma lucidum spore oil and ginseng seed oil were purchased from Guangdong Yue Microbial Technology Co., Ltd. The fermented oil of *Camellia sinensis* was prepared according to Example 2 in Chinese patent application CN122357302A, including: inoculating activated *Camellia sinensis* (derived from *Camellia sinensis* var. *sacchari*, strain name 1021, deposited at the China General Microbiological Culture Collection Center, accession number CGMCC No. 39106) into a basic fermentation medium containing camellia seed oil for fermentation culture to obtain a fermentation broth. After sterilization, the fermentation broth was allowed to stand to allow it to naturally separate into layers. The lower aqueous phase was separated, and the upper oil phase was collected to obtain the fermented oil of *Camellia sinensis* var. *sacchari*.

[0027] The antioxidant is tocopherol.

[0028] The preparation methods of the ternary oil compositions in the embodiments and comparative examples of the present invention include the following steps: Step 1: Weigh out Ganoderma lucidum spore oil, ginseng seed oil, and red camellia longevity fungus fermentation oil, and stir them thoroughly at 30°C at a stirring speed of ~200 rpm / min to obtain the composition; Step 2: Weigh the antioxidant, stir thoroughly at 30℃ at a stirring speed of ~200rpm / min, and the product is ready.

[0029] Examples 1-3 The specific weight parts of the ternary oil compositions in Examples 1-3 are shown in Table 1.

[0030] Table 1

[0031] Comparative Examples 1-5 The specific weight parts of the ternary oil compositions in Comparative Examples 1-5 are shown in Table 2.

[0032] Table 2

[0033] Test Example 1: Stability Assessment Stability testing was conducted according to T / GDCQMA 002-2023 "Specifications for Stability Testing of Cosmetics". Equal volumes of samples from Examples 1-3 and Comparative Examples 1-4 were placed in transparent, sealed plastic bottles (D0). Figure 1 As shown in Table 3, the appearance and odor were observed after being placed under high temperature (48℃) and low temperature (4℃) conditions for 3 months (D90).

[0034] Table 3

[0035] Test Example 2: IL-6 Anti-inflammatory Efficacy Test Bacterial lipopolysaccharide (LPS) stimulates monocytes and macrophages via TLR4, activating inflammatory signaling pathways such as NF-κB, thereby inducing the release of inflammatory factors such as IL-6 and exacerbating the inflammatory response. Using a classic cell model of LPS-induced inflammation in mouse RAW264.7 monocytes and macrophages, the difference in IL-6 levels between the negative control group (LPS only) and the sample group (LPS + sample) was compared to assess the inhibitory effect of the sample on IL-6 secretion, thus evaluating whether the sample has a soothing effect.

[0036] IL-6 levels were determined using an enzyme-linked immunosorbent assay (ELISA). The principle is as follows: IL-6 specifically binds to IL-6 antibodies coated on an ELISA plate, which then bind to substrate-labeled anti-IL-6 antibodies. The substrate is catalyzed by the enzyme, producing a colored product. The IL-6 level is positively correlated with the intensity of the colored product. The optical density (OD) was measured at 450 nm using an ELISA reader to calculate the IL-6 level.

[0037] Test method: RAW264.7 macrophages in logarithmic growth phase were seeded into 3 cm diameter cell culture plates or 6-well cell culture plates. After the cells reached 80% confluence, induction and drug administration were performed. The test wells were filled with culture medium containing a certain concentration of the test substance and LPS. The negative control wells were filled with cell culture medium containing LPS. The positive control wells were filled with culture medium containing the positive control and LPS. The blank / solvent control wells were filled with cell culture medium. After culturing for 24 h, the cell supernatant was collected, and the IL-6 content was detected using an ELISA kit.

[0038] IL-6 relative expression rate = T / C × 100%; In the formula: T is the average IL-6 content of the test substance; C is the average IL-6 content of the negative control.

[0039] Table 4. Relative expression levels of the IL-6 gene

[0040] Results: As shown in Table 4, at a test concentration of 0.1%, the IL-6 expression levels in Examples 1-3 were lower than those in Comparative Examples 1-4, indicating that the three oil compositions have the effect of inhibiting the production of macrophage inflammatory factors and have a soothing effect.

[0041] Test Example 3: COL1A1 Anti-wrinkle Test COL1A1 is a major component of skin collagen fibers and the most abundant collagen in human skin, playing a crucial role in skin elasticity and structural stability. Studies have shown that promoting COL1A1 expression can reduce skin lipid peroxidation, improve skin aging, and combat wrinkles, thereby improving skin quality. This experiment used real-time quantitative PCR (RT-qPCR) to detect the relative expression level of the target gene and quantitatively evaluate the anti-wrinkle efficacy of the composition.

[0042] Test Methods: 1. **Cells were passaged to the logarithmic growth phase, adjusted to an appropriate cell density, and seeded in 12-well plates. Incubation was carried out for 24 hours until confluence reached 70%-80%.** 2. **Sample Processing:** The culture medium was discarded, and complete culture medium containing the test sample (DMEM medium + 10% fetal bovine serum + 1% triple antibody) was added. The control group was added with an equal volume of complete culture medium, and incubated for 24 hours. 3. **RNA Extraction and Reverse Transcription:** The culture medium was discarded, and cells were washed twice with PBS. RNA was extracted from each group of cells according to the instructions of the COL1A1 gene content assay kit. After purity testing, cDNA was synthesized via reverse transcription. 4. **RT-qPCR (Real-time Quantitative PCR):** The reaction system (fluorescent probe + target gene primer + cDNA) was prepared, and the amplification program (denaturation-annealing-extension cycles 40 times) was set. The expression level of the COL1A1 gene in each group of cells was detected. 5. **Data Analysis:** The relative expression level of the target gene was calculated using the 2^AACt method (with GAPDH as an internal reference). The formula is as follows: Ct = Ct target gene - Ct internal reference; Ct = Ct treatment group - △Ct control group; Relative gene expression level = 2^-△△Ct.

[0043] Table 5. Relative expression levels of the COL1A1 gene.

[0044] Results: As shown in Table 5, at a test concentration of 0.25%, the relative expression levels of the COL1A1 gene in Examples 2 and 3 were greater than those in Example 1 and Comparative Examples 1-4, indicating that the three oil compositions can significantly promote the expression of the COL1A1 gene and have anti-wrinkle and firming effects.

[0045] Test Example 4: MMP-1 Test Matrix metalloproteinase-1 (MMP-1) is mainly involved in the degradation of type I and type III collagen. When MMP-1 is overexpressed, it specifically degrades extracellular matrix (ECM) components and destroys the normal structure of collagen and elastic fibers. Therefore, MMP-1 is the most important enzyme that causes aging symptoms such as wrinkles and fine lines on the skin.

[0046] Table 6 Results of MMP-1 gene expression level assay

[0047] Results: As shown in Table 6, at a test concentration of 0.25%, the relative expression levels of the MMP-1 gene in Examples 1-3 were lower than those in Comparative Examples 1-4, indicating that the three oil compositions can significantly inhibit the expression of the MMP-1 gene and have anti-wrinkle effects.

[0048] Test Example 5: Self-emulsifying ability Self-emulsifying emulsions were prepared from the compositions of Example 3 and Comparative Examples 1, 2, 3, and 5. The preparation steps included: 1. Taking 1 mL of each of the compositions of Example 3 and Comparative Examples 1, 2, 3, and 5 as the oil phase; 2. Taking 20 mL of the aqueous phase (deionized water) and keeping it at a constant temperature of 25°C; 3. Slowly adding the oil phase to the aqueous phase while magnetically stirring at 300-500 rpm for 5 min; 4. Immediately after obtaining the emulsion, taking a sample, dropping it onto a glass slide, covering it with a coverslip, and observing it under 40x magnification.

[0049] The results are as follows: Figure 2-6 As shown, under mild stirring conditions, the micrographs of the self-emulsifying emulsions formed in Comparative Examples 1, 2, 3, and 5 show that the droplet size is large and unevenly distributed, with obvious oil droplet aggregation or free oil phase. In contrast, the micrograph of the emulsion obtained from the ternary composite oil system in Example 3 shows that the droplet size is significantly reduced, the distribution is uniform and dense, there is no obvious aggregation or free oil phase, and the droplet morphology is regular and stable. This indicates that Ganoderma lucidum spore oil, ginseng seed oil, and Camellia sinensis fermentation oil can significantly enhance the self-emulsifying ability of the oil system through synergistic effects, promoting the formation of a delicate and stable emulsion system.

[0050] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various modifications or variations within the scope of the claims, which do not affect the essence of the present invention.

Claims

1. A ternary oil composition with highly efficient anti-wrinkle synergistic and self-emulsifying effects, characterized in that, It includes the following components in parts by weight: 1-5 parts Ganoderma lucidum spore oil, 1-5 parts ginseng seed oil, 1-15 parts fermented oil of red camellia longevity fungus, and 1-10 parts antioxidant.

2. The ternary oil composition according to claim 1, characterized in that, The total triterpenoid content in the Ganoderma lucidum spore oil is >30%.

3. The ternary oil composition according to claim 1, characterized in that, The ginseng seed oil contains linolenic acid and linoleic acid, wherein the mass percentage of linolenic acid is 0.01-1.4% and the mass percentage of linoleic acid is 0.1-19.4%.

4. The ternary oil composition according to claim 1, characterized in that, The preparation method of the red camellia longevity bacteria fermented oil includes: inoculating activated spore yeast into a basic fermentation medium containing red camellia seed oil for fermentation culture to obtain fermentation broth; then sterilizing the fermentation broth and letting it stand, allowing it to naturally separate into layers, collecting the upper oil phase to obtain red camellia longevity bacteria fermented oil.

5. The ternary oil composition according to claim 4, characterized in that, The basic fermentation medium includes: glucose 4-6 g / L, peptone 18-22 g / L, yeast powder 9-11 g / L, potassium dihydrogen phosphate 3-4 g / L, dipotassium hydrogen phosphate 4-6 g / L, diammonium hydrogen phosphate 3-4 g / L, sodium chloride 4-6 g / L, and magnesium sulfate heptahydrate 0.8-1.2 g / L; the amount of camellia seed oil added is 100%-200% of the mass of the basic fermentation medium.

6. A method for preparing a ternary oil composition as described in any one of claims 1-5, characterized in that, Includes the following steps: Step 1: Weigh out Ganoderma lucidum spore oil, ginseng seed oil, and red camellia longevity fungus fermented oil, stir well, and obtain the composition; Step 2: Add antioxidants and stir well to obtain the final product.

7. The preparation method according to claim 6, characterized in that, The stirring temperature is 20-40℃, and the stirring speed is 100-300 rpm / min.

8. Use of the ternary oil composition according to any one of claims 1-5 in the preparation of cosmetics.

9. A cosmetic product, characterized in that, The cosmetic contains 0.1 to 10 wt% of a ternary oil composition as described in any one of claims 1-5.

10. The cosmetic product according to claim 9, characterized in that, The cosmetics include one of the following: serum, lotion, facial mask, cream, sunscreen, makeup, and personal care products.

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