A composition with anti-inflammatory and gingival protection functions and application thereof

Abstract

The application discloses a composition with gingiva protection function and application thereof, and belongs to the technical field of daily chemicals. The composition comprises tremella extract, guaiacol sulfonate sodium and comfrey extract. The composition can be used for relieving inflammatory reaction and enhancing the immune defense ability of the gingiva, thereby playing a role in protecting the health of the gingiva.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products technology, specifically to a multi-effect oral care composition and its application that has anti-inflammatory and gum-protecting properties, repairs tooth enamel, and enhances the immune defense capabilities of the gums. The composition includes Tremella fuciformis extract, sodium guaiac sulfonate, and Lithospermum erythrorhizon extract. Through the synergistic effect of these components, it can effectively relieve gum inflammation and enhance gum health. Background Technology

[0002] Oral health is an important part of overall human health. Gingivitis, a common oral problem, is often accompanied by discomfort such as red, swollen, painful, and bleeding gums. If not treated in time, it may develop into more serious oral diseases, affecting the normal function and appearance of teeth.

[0003] With the increasing demand for oral health, the efficacy of traditional toothpaste in anti-inflammatory, antioxidant, and gum repair functions can no longer fully meet market needs. Free radical oxidative damage, bacterial infection, and chronic inflammation in the oral environment can easily lead to swollen and bleeding gums, and even periodontal tissue degeneration. Therefore, developing a multifunctional toothpaste formula that combines highly effective antioxidant properties, broad-spectrum antibacterial activity, rapid anti-inflammatory effects, and gum repair promotion is of great significance.

[0004] This patent proposes an innovative composition that achieves anti-inflammatory and gum-protective functions. The composition contains multiple active ingredients that exert anti-inflammatory and gum-health-protective effects at different levels.

[0005] Tremella fuciformis extract is rich in polysaccharides, polyphenols, and natural antioxidants, exhibiting excellent moisturizing, repairing, and free radical scavenging abilities. In the oral cavity, it effectively neutralizes reactive oxygen species (ROS), reduces oxidative stress damage to gingival tissue, and simultaneously forms a protective biofilm, promoting mucosal repair and enhancing gingival barrier function.

[0006] Sodium guaiac sulfonate, a highly effective anti-inflammatory and analgesic ingredient, can rapidly inhibit the release of gingival inflammatory mediators (such as IL-6 and TNF-α), relieve redness, swelling, and pain, and accelerate the healing of damaged tissues. Its unique sulfonated structure enhances water solubility and bioavailability, ensuring rapid action in the oral environment.

[0007] The allantoin and shikonin in comfrey extract have significant anti-inflammatory, swelling-reducing, and repair-promoting effects, acting directly on inflamed areas to reduce gingival tissue damage. Optimized extraction processes ensure that the pyrrolizidine alkaloids (PAs) content meets safety standards, balancing efficacy and safety.

[0008] Currently, existing oral care products on the market primarily use pharmaceutical ingredients such as cetirizine and chlorhexidine for anti-inflammatory and gum-protecting applications. However, with consumers becoming increasingly health-conscious, they are paying more and more attention to natural ingredients. Therefore, there is an urgent need to develop a highly effective, more natural, and gentle composition with anti-inflammatory and gum-protecting functions. Summary of the Invention

[0009] In view of the shortcomings of the prior art, the present invention provides a composition with anti-inflammatory and gingival protection functions and its application, which can relieve inflammatory response and enhance the immune defense of the gums, thereby playing a role in protecting gum health.

[0010] The technical solution of the present invention is as follows: A composition with antioxidant function is provided, comprising Tremella fuciformis extract, sodium guaiac sulfonate, and Lithospermum erythrorhizon extract.

[0011] Optionally, the dry weight ratio of the components of the nutrient composition in the nutritional supplement is as follows: Tremella fuciformis extract: 0.05-0.3%, sodium guaiacol sulfonate: 0.05-0.3%, and Lithospermum erythrorhizon extract: 0.2-1%.

[0012] The present invention also provides the application of a composition with anti-inflammatory and gingival-protecting functions in oral care products.

[0013] Optionally, the compositions of the present invention can be widely used in a variety of oral care products, including but not limited to toothpaste, mouthwash, oral gel, oral freshening spray, and oral freshener.

[0014] Optionally, when applied to toothpaste, the composition is added to the toothpaste at a rate of 3% to 5% of the total weight of the toothpaste. Through daily brushing, the active ingredients in the composition come into full contact with the gum tissue and oral mucosa, exerting their anti-inflammatory and gum-protecting effects, effectively preventing and treating gingivitis, and maintaining oral health.

[0015] Optionally, when applied to mouthwash, the composition is added to the mouthwash at a rate of 2% to 4% of the total weight of the mouthwash. When using the mouthwash, the gums and the entire oral cavity are cleaned and treated with anti-inflammatory properties.

[0016] The composition of this invention combines various natural plant extracts and compounds with specific functions, with each component working synergistically to alleviate gingival inflammation. Experiments at both the cellular and practical application levels have fully demonstrated its remarkable effects in reducing inflammation and protecting gums. Attached Figure Description

[0017] Appendix Figure 1The figure shows the effect of the composition in Example 1 on the levels of inflammatory factors (IL-6, TNF-α) in the culture supernatant of inflamed gingival fibroblasts.

[0018] Appendix Figure 2 The figure shows the effect of the composition in Example 2 on intracellular oxidative stress indicators (SOD activity and MDA content) in inflamed gingival fibroblasts.

[0019] Appendix Figure 3 The figure shows the effect of the composition in Example 3 on the viability of inflamed gingival fibroblasts. Detailed Implementation

[0020] The technical content of the present invention will be further described below with reference to the embodiments. The following embodiments are illustrative and not limiting, and should not be used to limit the scope of protection of the present invention. Unless otherwise specified, the experimental methods used in the following embodiments are conventional methods. Unless otherwise specified, the materials and reagents used in the following embodiments are commercially available. Example 1

[0021] Toothpaste Formulation: A toothpaste containing the composition of the present invention is prepared, the formulation of which is as follows (by weight): abrasive (e.g., calcium carbonate): 40%; humectant (e.g., glycerin): 25%; thickener (e.g., sodium carboxymethyl cellulose): 2%; surfactant (e.g., sodium dodecyl sulfate): 3%; composition of the present invention (Tremella fuciformis extract, sodium guaiac sulfonate, and Lithospermum erythrorhizon extract in the above-specified weight ratio): 5%; fragrance: 1%; preservative: 0.5%; sweetener: 0.5%.

[0022] Experimental Methods: Gingival fibroblast culture: Gingival fibroblasts were obtained from gingival tissue of healthy volunteers and cultured in DMEM medium containing 10% fetal bovine serum and 1% penicillin-streptomycin at 37 °C, 5% CO2, and 95% relative humidity. Cells were used in subsequent experiments after reaching the logarithmic growth phase. Establishment of an inflammation model: Lipopolysaccharide (LPS) was added to the cultured gingival fibroblasts at a final concentration of 1 μg / mL to induce an inflammatory response, simulating a gingival inflammation state.

[0023] The toothpaste of this invention was prepared into an extract containing the active ingredients. The specific method is as follows: Take an appropriate amount of toothpaste, add an appropriate amount of PBS buffer, vortex to mix, centrifuge, and take the supernatant as the extract. The extract was added to gingival fibroblasts with established inflammation model according to different concentration gradients (0.1%, 0.5%, 1%, 2%). At the same time, a blank control group (PBS buffer only) and a positive control group (ibuprofen: 15 ug / mL) were set up.

[0024] The levels of inflammatory factors (interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)) in cell culture supernatant were detected using ELISA. Cell culture supernatant was collected 24 hours after drug treatment, and the ELISA kit was used for detection according to the instructions. Lower levels of inflammatory factors indicated better anti-inflammatory effects. The effect of the toothpaste extract of this invention on the secretion of inflammatory factors was evaluated by comparing with a blank control group and a positive control group.

[0025] It was observed that the toothpaste extract of this invention significantly reduced the levels of IL-6 and TNF-α in cell culture supernatant. At a concentration of 0.5%, the IL-6 concentration decreased from 150 pg / mL in the blank control group to 80 pg / mL, and the TNF-α concentration decreased from 120 pg / mL in the blank control group to 60 pg / mL, showing a better anti-inflammatory effect compared to the positive control group. This fully demonstrates that the toothpaste extract of this invention can effectively inhibit the secretion of inflammatory factors, thereby exerting an anti-inflammatory effect. Example 2

[0026] Mouthwash formulation: A mouthwash containing the composition of the present invention is prepared, the formulation of which is as follows (by weight): water: 80%; alcohol: 5%; composition of the present invention (Tremella fuciformis extract, sodium guaiac sulfonate, and Lithospermum erythrorhizon extract in the weight ratio specified above): 4%; fragrance: 1%; preservative: 0.5%; buffer (disodium hydrogen phosphate): 0.5%.

[0027] Experimental Methods: Gingival fibroblast culture: Gingival fibroblasts were obtained from gingival tissue of healthy volunteers and cultured in DMEM medium containing 10% fetal bovine serum and 1% penicillin-streptomycin at 37 °C, 5% CO2, and 95% relative humidity. Cells were used in subsequent experiments after reaching the logarithmic growth phase. Establishment of an inflammation model: Lipopolysaccharide (LPS) was added to the cultured gingival fibroblasts at a final concentration of 1 μg / mL to induce an inflammatory response, simulating a gingival inflammation state.

[0028] The mouthwash of this invention was prepared into an extract containing the effective ingredients. The specific method is as follows: Take an appropriate amount of mouthwash, add an appropriate amount of PBS buffer, vortex to mix, centrifuge, and take the supernatant as the extract. The extract was added to gingival fibroblasts with established inflammation model according to different concentration gradients (0.1%, 0.5%, 1%, 2%). At the same time, a blank control group (PBS buffer only) and a positive control group (ibuprofen: 15 ug / mL) were set up.

[0029] Oxidative stress marker detection: Intracellular oxidative stress markers (superoxide dismutase (SOD) activity and malondialdehyde (MDA) content) were detected using appropriate kits. Cells were collected 24 hours after drug treatment, and the tests were performed according to the kit instructions. Higher SOD activity and lower MDA content indicated better cellular oxidative stress. The effect of the mouthwash extract of this invention on cellular oxidative stress was evaluated by comparing with a blank control group and a positive control group.

[0030] It was observed that the mouthwash extract of this invention can increase intracellular SOD activity and reduce MDA content. At a concentration of 0.5%, SOD activity increased from 20 U / mL in the blank control group to 28 U / mL, and MDA content decreased from 10 nmol / mL in the blank control group to 6 nmol / mL. This indicates that the mouthwash extract of this invention has a good effect on improving cellular oxidative stress, helping to reduce cell damage caused by oxidative stress, and thus promoting the health of gingival tissue. Example 3

[0031] An oral care spray containing the composition of the present invention is prepared, the formulation of which is as follows (by weight): solvent matrix (such as purified water, glycerin, etc.): 30 parts; moisturizer (such as sodium hyaluronate, etc.): 20 parts; composition of the present invention (Tremella fuciformis extract, sodium guaiac sulfonate, and Lithospermum erythrorhizon extract, in the weight ratio specified above): 8 parts; fragrance: 1 part; preservative: 0.5 parts.

[0032] Experimental Methods: Gingival fibroblast culture: Gingival fibroblasts were obtained from gingival tissue of healthy volunteers and cultured in DMEM medium containing 10% fetal bovine serum and 1% penicillin-streptomycin at 37°C, 5% CO2, and 95% relative humidity. Cells were used in subsequent experiments after reaching the logarithmic growth phase. Establishment of an inflammation model: Lipopolysaccharide (LPS) was added to the cultured gingival fibroblasts at a final concentration of 1 μg / mL to induce an inflammatory response, simulating gingival inflammation.

[0033] Drug processing: The oral care spray of this invention was prepared into an extract containing the effective ingredients. The specific method is as follows: Take an appropriate amount of mouthwash, add an appropriate amount of PBS buffer, vortex to mix, centrifuge, and take the supernatant as the extract. The extract was added to gingival fibroblasts with established inflammation model according to different concentration gradients (0.1%, 0.5%, 1%, 2%). At the same time, a blank control group (PBS buffer only) and a positive control group (ibuprofen: 15 ug / mL) were set up.

[0034] Cell viability assay: Cell viability was assessed using the MTT assay. At a specific time point (24 hours) after drug treatment, MTT solution (5 mg / mL) was added to each well, and after 4 hours of incubation, the supernatant was discarded, and DMSO was added to dissolve the crystals. The absorbance at 570 nm was measured using a microplate reader. Higher absorbance values ​​indicated higher cell viability. The effect of the oral care spray extract of this invention on the viability of inflammatory cells was evaluated by comparing it with a blank control group and a positive control group.

[0035] As can be clearly seen from the figure, cell viability gradually increases with the increase of the concentration of the oral care spray extract. At a concentration of 1%, cell viability is significantly improved compared with the blank control group, and the absorbance value increases from 0.35 in the blank control group to 0.75, indicating that the oral care spray extract of the present invention has a significant promoting effect on the viability of inflamed gingival fibroblasts at this concentration.

[0036] The preferred embodiments of the present invention have been described in detail above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the scope of protection defined by the claims.

Claims

1. A composition with anti-inflammatory and gingival-protecting functions, characterized in that, The composition includes Tremella fuciformis extract, sodium guaiac sulfonate, and Lithospermum erythrorhizon extract.

2. The composition according to claim 1, characterized in that, It includes the following components by weight percentage: Tremella fuciformis extract: 0.05 ~ 0.3%, sodium guaiac sulfonate: 0.05 ~ 0.3%, and Lithospermum erythrorhizon extract: 0.2 ~ 1%.

3. The composition according to claim 1 or 2, characterized in that, The composition is an oral care composition for relieving inflammatory responses.

4. The gingival composition with anti-inflammatory and anti-inflammatory properties according to claim 1 or 2, characterized in that, The composition also includes a carrier or excipient acceptable for oral care products.

5. The composition according to claim 4, characterized in that: Acceptable carriers or excipients for the oral care products include water, hydrated silica, sorbitol, glyceryl polyethylene glycol-8, sodium lauryl sulfate, flavoring, cocamidopropyl betaine, xanthan gum, benzyl alcohol, tetrasodium pyrophosphate, sodium saccharin, dextranase, PVM / MA copolymer, nicotinamide, trichlorogalactosyl menthol, menthol ethylamine, and calcium aluminum borosilicate.

6. The use of the composition according to claim 1 or 2 in the preparation of oral care products.

7. The application as claimed in claim 6, characterized in that, The composition is formulated into oral care products, including any one of toothpaste, mouthwash, oral gel, oral care spray, and mouth freshener.

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