A pharma-food extract for repairing damaged pancreatic islet cells and preparation and application thereof

Abstract

The application belongs to the technical field of traditional Chinese medicine research and development, and discloses a medicine-food homologous extract for repairing damaged pancreatic islet cells, which comprises the following components in parts by weight: radix notoginseng 15-35g, cistanche 15-60g, fructus corni 12-30g, honeysuckle 20-50g, leek flower 30-60g, dendrobium officinale 25-60g, mulberry leaf 25-60g, eucommia leaf 15-50g, chlorella 15-50g, ginseng 15-60g, bitter gourd 20-50g, saffron 15-50g, red citrus 15-50g, gamma-aminobutyric acid 9-30g, bee pollen 20-60g, corn silk 15-60g, arabinose 15-50g, radix puerariae 25-60g, celery seed 20-60g, powder 10-20g, capsule 15-25g, oral liquid 50-100g, and tablet candy 5-10g. Through the fine steps, including strict selection and pretreatment of raw materials, accurate crushing and mixing, appropriate selection and preparation of solvents, and efficient extraction and concentration, purification and separation technology, the excellent quality and safety of the extract are ensured.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine research and development technology, specifically a medicinal and edible extract for repairing damaged pancreatic islet cells, its preparation and application. Background Technology

[0002] A medicinal and edible extract for repairing damaged pancreatic islet cells refers to natural substances that possess both medicinal efficacy and can be used as a food source. These substances are extracted from plants, animals, or microorganisms through specific preparation processes, and contain active ingredients that can effectively repair damaged pancreatic islet cells and improve their function. The preparation and application of this extract falls within the field of traditional Chinese medicine research and development, and also involves fields such as nutrition and food science. Current technologies face major problems with traditional extraction methods, including inappropriate solvent selection, insufficient extraction conditions, improper raw material processing, and technological limitations. If the polarity and solubility of the solvent do not match the target component, it will directly lead to low extraction efficiency. Simultaneously, improper control of extraction conditions such as temperature, pressure, and time may also cause insufficient dissolution or decomposition of the target component, thereby reducing extraction efficiency. The pretreatment and pulverization degree of the raw materials are equally crucial. Improper treatment or insufficient pulverization will prevent the solvent from fully penetrating the raw materials, affecting the extraction effect. Furthermore, impurities and moisture in the raw materials are also important factors affecting extraction efficiency. Regarding technological limitations, insufficient equipment precision or unskilled operators may increase errors during the extraction process, further affecting extraction efficiency. Summary of the Invention

[0003] The purpose of this invention is to provide a medicinal and edible extract for repairing damaged pancreatic islet cells, its preparation and application, in order to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, the present invention provides the following technical solution: a medicinal and edible extract for repairing damaged pancreatic islet cells, comprising the following components in parts by weight: 15-35g of Panax notoginseng, 15-60g of Cistanche deserticola, 12-30g of Cornus officinalis, 20-50g of Lonicera japonica, 30-60g of Allium tuberosum flower, 25-60g of Dendrobium officinale, 25-60g of Morus alba leaf, 15-50g of Eucommia ulmoides leaf, 15-50g of Chlorella vulgaris, and 15-60g of Panax ginseng. Bitter melon 20-50g, saffron 15-50g, tangerine peel 15-50g, γ-aminobutyric acid 9-30g, bee pollen 20-60g, corn silk 15-60g, arabinose 15-50g, kudzu root 25-60g, celery seed 20-60g, powder 10-20g, capsules 15-25g, oral liquid 10-20g, compressed candy 5-10g, powder 15g, capsules 20g, oral liquid 20g, compressed candy 7.5g.

[0005] Preferably, the specific steps of the preparation are as follows: S1: Raw material selection and pretreatment: Select raw materials carefully, wash and remove impurities, and dry them to ensure purity and suitability for extraction;

[0006] S2: Grinding and Mixing: Finely grind and dry raw materials, and mix them in precise proportions to ensure a balanced composition of the extract.

[0007] S3: Preparation of extraction solvent: Select a suitable solvent, prepare it according to the ratio, and ensure its purity and suitability;

[0008] S4: Extraction and Concentration: Extraction with mixed raw materials using solvent, selecting reflux extraction or percolation extraction methods; concentrate the extract and remove the solvent.

[0009] S5: Purification and Separation: Purify the extract to remove impurities and ensure the purity of the components.

[0010] S6: Drying and Packaging: Drying to obtain a solid, packaging to ensure safety: processing, storage and transportation stability of the purified extract;

[0011] S7: Quality Inspection and Evaluation: Quality inspection evaluates the effectiveness to ensure that the extract meets the standards and that safety and purity are guaranteed.

[0012] Preferably, the raw material selection and pretreatment in S1 refers to selecting 35g of Panax notoginseng, 60g of Cistanche deserticola, 30g of Cornus officinalis, 50g of Lonicera japonica, 60g of Allium tuberosum, 60g of Dendrobium officinale, 60g of Morus alba leaves, 50g of Eucommia ulmoides leaves, 50g of Chlorella vulgaris, 60g of Panax ginseng, 50g of Melia toosendan, 50g of Saffron, 50g of Citrus reticulata peel, 30g of GABA, 60g of bee pollen, 60g of corn silk, 50g of arabinose, 60g of Pueraria lobata, and 60g of Celery seed, and performing pretreatment such as washing, removing impurities, and drying to ensure the purity and suitability of the raw materials.

[0013] Preferably, the specific steps of crushing and mixing in S2 are as follows:

[0014] Step 1: Fine grinding: Place each dried raw material into a grinder separately and adjust to the appropriate particle size to ensure that the raw material powder is fine and easy to dissolve, in preparation for the subsequent extraction process;

[0015] Step 2: Precise Proportioning: Based on the usage range of each raw material, use precise measuring tools, such as an electronic scale, to weigh each raw material according to the predetermined ratio, ensuring that the proportion of each component is accurate;

[0016] Step 3: Uniform Mixing: Finally, put the weighed raw material powders into a mixing container and mix them evenly using a stirrer or by hand to ensure that the proportions of each component in the extract are balanced, so as to improve the extraction effect.

[0017] Preferably, the solvent preparation in S3 refers to fully considering the properties of the raw materials and the specific extraction requirements when selecting a solvent. For certain specific active ingredients, ethanol is the first choice due to its good solubility and penetrability; while for other ingredients, water or mixed solvents are more suitable. After selecting a solvent, it is necessary to prepare it strictly according to a certain ratio. At the same time, the purity of the solvent is ensured by means of filtration and distillation, and the optimal suitability is achieved by adjusting the temperature and pH parameters.

[0018] Preferably, the specific steps for extraction and concentration in S4 are as follows:

[0019] Step 1: Mixing and Extraction: Combine the well-mixed raw materials with the selected solvent and use reflux extraction or percolation extraction methods to ensure that the effective components in the raw materials are fully dissolved in the solvent;

[0020] Step 2: Extraction collection: After the extraction process is completed, carefully collect the extract containing the active ingredients, taking care to avoid loss and contamination;

[0021] Step 3: Concentration treatment: The collected extract is concentrated by removing excess solvent through evaporation, and finally a concentrated extract containing high concentration of active ingredients is obtained.

[0022] Preferably, the specific steps of purification and separation in S5 are as follows:

[0023] Step 1: Purification of the concentrated extract:

[0024] Place the concentrated extract into a purification device and operate according to the specific requirements of either macroporous resin adsorption purification or membrane separation purification.

[0025] When using macroporous resin adsorption, it is necessary to select a suitable resin type and adjust the appropriate pH value and concentration conditions so that the target component is adsorbed by the resin, while impurities are removed with the effluent.

[0026] When using membrane separation technology, appropriate membrane modules and operating pressures are selected based on the pore size and molecular weight cutoff of the membrane, and impurities and unwanted components are removed through membrane filtration.

[0027] Step 2: Separation of the purified extract:

[0028] After purification, the resulting extract may still contain various components. In this case, it is necessary to select an appropriate separation method based on the properties of the target components and the separation requirements.

[0029] Commonly used separation methods include solvent extraction, chromatographic separation, and electrophoresis. Appropriate separation conditions and operating parameters are selected based on the characteristics of the target component.

[0030] The target component is separated from the purified extract through a separation step, resulting in a target component or component with higher purity.

[0031] Step 3: Collection and Post-processing:

[0032] After the separation step is completed, the target component or part should be collected promptly and properly stored.

[0033] Based on subsequent application requirements, the collected target components or parts are further processed, such as drying, crystallization, and recrystallization, to improve their purity and stability.

[0034] At the same time, the waste generated during the separation process is classified and treated to ensure compliance with environmental protection requirements.

[0035] Preferably, in step S6, drying and packaging refers to the post-processing stage of the extraction process, where the purified extract needs to be dried to convert the liquid extract into a solid form. Advanced drying technologies, such as spray drying and vacuum drying, ensure that the extract retains its original active ingredients and quality during the drying process. Subsequently, the obtained solid extract is carefully packaged using high-performance materials that are moisture-proof, oxidation-proof, and light-proof to ensure its stability and safety during long-term storage and transportation, providing customers with high-quality products.

[0036] Preferably, S7 refers to quality inspection and evaluation. In the production process of extract, quality inspection is a key link to ensure that the quality meets the standards. It comprehensively covers the core indicators of component content, purity and safety. With the help of advanced technology and equipment, the content of each component is accurately measured, the purity is evaluated and potential safety risks are investigated. After quality inspection, the quality and effect of the extract are comprehensively evaluated based on the results to ensure that it meets industry standards and customer needs and guarantees the actual application effect.

[0037] Preferably, the medicinal and edible extract for repairing damaged pancreatic islet cells based on the above method can be applied to drug development, functional foods, health products, and medical research.

[0038] The beneficial effects of this invention are as follows:

[0039] This invention, through meticulous steps including rigorous selection and pretreatment of raw materials, precise pulverization and mixing, appropriate selection and preparation of solvents, and efficient extraction, concentration, purification, and separation technologies, ensures the superior quality and safety of the extract. The preparation utilizes a variety of natural medicinal herbs and nutrients, which undergo deep cleaning, impurity removal, and drying to guarantee the purity and suitability of the raw materials. During the extraction process, reflux or percolation techniques are employed to ensure the full dissolution of the active ingredients, and a high-concentration extract is obtained through concentration. Subsequently, purification and separation further enhance the purity of the extract. Finally, advanced drying technology and high-performance packaging ensure the stability during long-term storage and transportation. Comprehensive quality testing and evaluation ensure that the extract meets standards and customer needs, providing strong support for drug development, functional foods, health products, and medical research, particularly demonstrating great potential in repairing damaged pancreatic islet cells and improving diabetes. Attached Figure Description

[0040] Figure 1 This is a schematic diagram of the overall usage process of the present invention. Detailed Implementation

[0041] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0042] like Figure 1 As shown, this embodiment of the invention provides a medicinal and edible extract for repairing damaged pancreatic islet cells. This extract comprises the following components in parts by weight: 15-35g of Panax notoginseng, 15-60g of Cistanche deserticola, 12-30g of Cornus officinalis, 20-50g of Lonicera japonica, 30-60g of Allium tuberosum flower, 25-60g of Dendrobium officinale, 25-60g of Morus alba leaf, 15-50g of Eucommia ulmoides leaf, and 15-50g of Chlorella vulgaris. Ginseng 15-60g, bitter melon 20-50g, saffron 15-50g, tangerine peel 15-50g, γ-aminobutyric acid 9-30g, bee pollen 20-60g, corn silk 15-60g, arabinose 15-50g, kudzu root 25-60g, celery seed 20-60g, powder 10-20g, capsules 15-25g, oral liquid 10-20g, compressed candy 5-10g.

[0043] Weighing: 35g Panax notoginseng, 60g Cistanche deserticola, 30g Cornus officinalis, 50g Lonicera japonica, 60g Allium tuberosum, 60g Dendrobium officinale, 60g Mulberry leaf, 50g Eucommia ulmoides leaf, 50g Chlorella vulgaris, 60g Ginseng, 50g Bitter melon, 50g Saffron, 50g Citrus reticulata peel, 30g GABA, 60g Bee pollen, 60g Corn silk, 50g Arabinose, 60g Pueraria lobata, 60g Celery seed, 10g Powder, 15g Capsules, 10g Oral Liquid, 5g Compressed Candy.

[0044] Weighing: 15g Panax notoginseng, 15g Cistanche deserticola, 12g Cornus officinalis, 20g Lonicera japonica, 30g Allium tuberosum flowers, 25g Dendrobium officinale, 25g Morus alba leaves, 15g Eucommia ulmoides leaves, 15g Chlorella vulgaris, 15g Ginseng, 20g Bitter melon, 15g Saffron, 15g Citrus reticulata peel, 9g GABA, 20g Bee pollen, 15g Corn silk, 15g Arabinose, 25g Pueraria lobata, 20g Celery seed, 15g Powder, 20g Capsules, 15g Oral liquid, 7.5g Compressed candy.

[0045] Weigh the following ingredients: 25g Panax notoginseng, 35g Cistanche deserticola, 21g Cornus officinalis, 35g Lonicera japonica, 45g Allium tuberosum flowers, 25-60g Dendrobium officinale, 25-60g Mulberry leaves, 15-50g Eucommia ulmoides leaves, 38g Chlorella vulgaris, 45g Ginseng, 35g Bitter melon, 42g Saffron, 35g Citrus reticulata peel, 18g GABA, 40g Bee pollen, 42g Corn silk, 35g Arabinose, 30g Pueraria lobata, 40g Celery seeds, 20g Powder, 25g Capsules, 20g Oral Liquid, and 10g Compressed Candy.

[0046] The specific steps of the preparation are as follows:

[0047] S1: Raw material selection and pretreatment: Select raw materials carefully, clean them to remove impurities and dry them to ensure purity and suitability for extraction;

[0048] S2: Grinding and Mixing: Finely grind and dry raw materials, and mix them in precise proportions to ensure a balanced composition of the extract.

[0049] S3: Preparation of extraction solvent: Select a suitable solvent, prepare it according to the ratio, and ensure its purity and suitability;

[0050] S4: Extraction and Concentration: Extraction with mixed raw materials using solvent, selecting reflux extraction or percolation extraction methods; concentrate the extract and remove the solvent.

[0051] S5: Purification and Separation: Purify the extract to remove impurities and ensure the purity of the components.

[0052] S6: Drying and Packaging: Drying to obtain a solid, packaging to ensure safety: processing, storage and transportation stability of the purified extract;

[0053] S7: Quality Inspection and Evaluation: Quality inspection evaluates the effectiveness to ensure that the extract meets the standards and that safety and purity are guaranteed.

[0054] Quality testing: Testing the quality of the extract, including testing for component content, purity, safety, etc.

[0055] Assessment: Based on the test results, the quality and efficacy of the extract are assessed to ensure that it meets relevant standards and requirements.

[0056] In S1, the selection and pretreatment of raw materials refers to the selection of 35g of Panax notoginseng, 60g of Cistanche deserticola, 30g of Cornus officinalis, 50g of Lonicera japonica, 60g of Allium tuberosum, 60g of Dendrobium officinale, 60g of Morus alba leaves, 50g of Eucommia ulmoides leaves, 50g of Chlorella vulgaris, 60g of Panax ginseng, 50g of Melia toosendan, 50g of Saffron, 50g of Citrus reticulata peel, 30g of GABA, 60g of bee pollen, 60g of corn silk, 50g of arabinose, 60g of Pueraria lobata, and 60g of Celery seed, followed by washing, impurity removal, and drying to ensure the purity and suitability of the raw materials.

[0057] Raw material selection and pretreatment are the primary steps in ensuring the quality of the extract. Carefully selected natural medicinal herbs and nutrients, such as Panax notoginseng, Cistanche deserticola, and Cornus officinalis, are meticulously cleaned, purified, and dried to effectively remove impurities and undesirable components, ensuring the purity and suitability of the raw materials. This step not only enhances the safety and stability of the extract but also lays a solid foundation for its subsequent efficient extraction and high-quality preparation. Simultaneously, precise raw material proportions ensure a balanced ratio of components in the extract, contributing to optimal pharmacological effects and nutritional value.

[0058] The specific steps of crushing and mixing in S2 are as follows:

[0059] Step 1: Fine grinding: Place each dried raw material into a grinder separately and adjust to the appropriate particle size to ensure that the raw material powder is fine and easy to dissolve, in preparation for the subsequent extraction process;

[0060] Step 2: Precise Proportioning: Based on the usage range of each raw material, use precise measuring tools, such as an electronic scale, to weigh each raw material according to the predetermined ratio, ensuring that the proportion of each component is accurate;

[0061] Step 3: Uniform Mixing: Finally, put the weighed raw material powders into a mixing container and mix them evenly using a stirrer or by hand to ensure that the proportions of each component in the extract are balanced, so as to improve the extraction effect.

[0062] Fine grinding, precise proportioning, and uniform mixing are key steps in ensuring the quality of extracts. Fine grinding results in fine, easily soluble raw material powder, providing a good foundation for subsequent extraction. Precise proportioning ensures accurate dosage of each raw material, avoiding component deviations and guaranteeing the stability and consistency of the extract. Uniform mixing further ensures a balanced ratio of components in the extract, improving extraction efficiency and resulting in a final product with higher bioavailability and better therapeutic effects. The implementation of this series of steps lays a solid foundation for preparing high-quality, high-efficiency extracts.

[0063] In the S3 step, solvent preparation refers to the need to fully consider the properties of the raw materials and the specific requirements of extraction when selecting a solvent. For certain active ingredients, ethanol is the first choice due to its good solubility and penetrability; while for other ingredients, water or mixed solvents are more suitable. After selecting a solvent, it is necessary to prepare it strictly according to a certain ratio. At the same time, the purity of the solvent is ensured by means of filtration, distillation, etc., and the temperature and pH parameters are adjusted to achieve optimal suitability, creating favorable conditions for the subsequent extraction process.

[0064] Choosing a suitable solvent and preparing it carefully can significantly improve the extraction efficiency and quality of S3. Solvents such as ethanol are particularly effective for extracting specific active ingredients due to their good solubility and penetrating power; while water or mixed solvents are suitable for other components. Strictly controlling parameters such as solvent ratio, purity, temperature, and pH can optimize extraction conditions, create favorable conditions for subsequent processes, ensure the high quality and safety of the extract, and meet the needs of scientific research, medical treatment, and other fields.

[0065] The specific steps for extraction and concentration in S4 are as follows:

[0066] Step 1: Mixing and Extraction: Combine the well-mixed raw materials with the selected solvent and use reflux extraction or percolation extraction methods to ensure that the effective components in the raw materials are fully dissolved in the solvent;

[0067] Step 2: Extraction collection: After the extraction process is completed, carefully collect the extract containing the active ingredients, taking care to avoid loss and contamination;

[0068] Step 3: Concentration treatment: The collected extract is concentrated by removing excess solvent through evaporation, and finally a concentrated extract containing high concentration of active ingredients is obtained.

[0069] The specific steps of the reflux extraction method are as follows:

[0070] Experimental preparation:

[0071] Prepare the reflux extraction apparatus, including the reflux extractor, condenser, heater, etc., and ensure that the apparatus is properly connected and well-sealed.

[0072] Choose an appropriate solvent based on the experimental requirements and the properties of the substance being extracted, such as ethanol or water.

[0073] Prepare the sample of the substance to be extracted, ensuring that the sample is free of impurities, dry, and suitable for reflux extraction.

[0074] Addition of sample and solvent:

[0075] Add the sample of the substance to be extracted into the reflux extractor.

[0076] Add an appropriate amount of solvent to completely submerge the sample. The amount of solvent should be determined based on the sample volume and extraction requirements.

[0077] Device and heating:

[0078] Attach the reflux extractor to the heater and adjust the heater temperature to bring the solvent to a boil.

[0079] Ensure the condenser is connected correctly so that the solvent vapor can condense and flow back into the extractor.

[0080] Reflux extraction:

[0081] When the solvent begins to boil, the solvent vapor condenses into liquid through the condenser and then flows back to the reflux extractor.

[0082] The refluxed liquid comes into contact with the sample, achieving extraction. This process is repeated until the predetermined extraction time or extraction effect is achieved.

[0083] Control extraction conditions:

[0084] The temperature and time of reflux extraction are controlled according to the experimental requirements and the properties of the extracted substance.

[0085] The temperature should be moderate, avoiding excessively high temperatures that could damage the sample or excessively low temperatures that could affect extraction efficiency.

[0086] Extraction time typically ranges from 30 minutes to several hours, depending on the nature of the sample and the extraction requirements.

[0087] End of extraction and separation:

[0088] Once the extraction time is reached, turn off the heater and stop the reflux extraction.

[0089] The extract in the reflux extractor is separated from the solvent. This can be done by methods such as centrifugation and filtration.

[0090] Follow-up processing:

[0091] The separated extract is further processed, such as by evaporating the solvent and drying, to obtain a pure extract.

[0092] When evaporating the solvent, care should be taken to control the temperature and pressure to avoid damaging or deteriorating the extract.

[0093] Laboratory Safety and Cleanliness:

[0094] During the experiment, attention should be paid to safe operation to avoid solvent evaporation and leakage, and to avoid contact with fire sources.

[0095] After the experiment, the experimental equipment should be cleaned in a timely manner and the experimental environment should be kept clean.

[0096] The specific steps of the percolation extraction method are as follows:

[0097] The specific steps of the percolation extraction method typically include pulverizing the medicinal materials, wetting, loading into a container, degassing, soaking, percolation, and liquid collection and treatment. A detailed explanation follows:

[0098] Herbal Grinding: After drying the herbs to be extracted, grind them to an appropriate particle size using a grinder. The particle size should be suitable; if it is too fine, it will easily clog, increase adsorption, and result in poor leaching; if it is too coarse, it will be difficult to compact, resulting in a small contact area between the solvent and the herbs, which is also not conducive to leaching. Generally, the medium or coarse powder specifications specified in the Chinese Pharmacopoeia are preferred.

[0099] Powder wetting: Before loading the percolator, wet the powder with an extraction solvent (such as ethanol) to prevent the powder from expanding and causing blockage in the percolator, which would affect the percolation operation. Usually, add about one times the amount of solvent as the powder, mix well, and then, depending on the texture of the powder, seal and let it stand for a period of time (such as 15 minutes to 6 hours) until the powder is fully and evenly wetted and swelled.

[0100] Prepare the percolation cylinder: Place a layer of absorbent cotton or gauze at the bottom of the percolation cylinder to prevent the powder from clogging the bottom. Then, install the percolation cylinder on the fixing device, ensuring it is secure.

[0101] Filling and Compacting: Add the moistened medicinal powder into the percolation cylinder in several batches, pressing it down evenly after each addition to maintain consistent density. If the filling is too loose, the solvent will quickly flow through the powder, resulting in incomplete leaching; conversely, overfilling will clog the outlet, preventing percolation. The amount of powder added should not exceed 2 / 3 of the percolation cylinder's height. After filling, cover the powder surface with filter paper or gauze, then place a heavy object (such as a stone) to compact it, ensuring the medicine is evenly compressed during percolation.

[0102] Venting: Open the outlet valve at the bottom of the percolation tank and slowly add solvent (such as ethanol) from the top to maximize the removal of air from the gaps between the powder particles. Once the solution has flowed out, close the outlet valve. This step ensures that the solvent always submerges the powder surface, preventing the powder from drying and cracking, which would affect the leaching effect.

[0103] Static soaking: Add solvent to a level several centimeters above the medicinal material, then cover and soak for a period of time (usually 24–48 hours) to allow the solvent to fully penetrate and diffuse into the medicinal material. This step is particularly important for preparing high-concentration formulations.

[0104] Percolation: After the specified soaking time, open the outlet valve at the bottom of the percolation cylinder to begin percolation. Control the percolation rate within the specified range; generally, 1-3 mL per minute per 1000g of medicinal material is ideal. If the percolation rate is too fast, the active ingredients will not have enough time to percolate and diffuse, resulting in a low concentration of the extract; if it is too slow, it will affect equipment utilization and output. During the percolation process, the solvent level must always be kept above the surface of the medicinal material to prevent it from drying out and cracking. Simultaneously, the percolation rate should be checked regularly and the solvent replenished as needed.

[0105] Liquid collection and treatment: Collect the percolate according to the preparation requirements. If preparing a fluid extract, first collect 85% of the initial percolate of the medicinal material and store it separately. The subsequent percolate is concentrated at low temperature and then combined with the initial percolate to adjust to the specified standard. If preparing a tincture or other extract with a lower concentration, directly collect 3 / 4 of the percolate equivalent to the amount to be prepared and stop percolation. Press the residue and combine the pressed liquid with the percolate. Then add an appropriate amount of solvent to the required concentration and volume, let it stand, and filter to obtain the final product.

[0106] The specific steps of purification and separation in S5 are as follows:

[0107] Step 1: Purification of the concentrated extract:

[0108] Place the concentrated extract in a purification device and operate according to the specific requirements of the selected purification method (such as macroporous resin adsorption or membrane separation).

[0109] If macroporous resin adsorption is used, it is necessary to select the appropriate resin type and adjust the pH value and concentration conditions to allow the target component to be adsorbed by the resin, while impurities are removed with the effluent.

[0110] If membrane separation technology is used, appropriate membrane modules and operating pressures are selected based on the membrane pore size and molecular weight cutoff, and impurities and unwanted components are removed through membrane filtration.

[0111] Step 2: Separation of the purified extract:

[0112] After purification, the resulting extract may still contain multiple components. In this case, it is necessary to select an appropriate separation method based on the properties of the target components and the separation requirements.

[0113] Commonly used separation methods include solvent extraction, chromatographic separation (such as liquid chromatography and gas chromatography), and electrophoresis. Appropriate separation conditions and operating parameters are selected based on the characteristics of the target component.

[0114] The target component is separated from the purified extract through a separation step, resulting in a target component or component with higher purity.

[0115] Step 3: Collection and Post-processing:

[0116] After the separation step is completed, the target component or part should be collected in a timely manner and properly stored.

[0117] Based on subsequent application requirements, the collected target components or parts are further processed, such as drying, crystallization, and recrystallization, to improve their purity and stability.

[0118] At the same time, the waste generated during the separation process is classified and treated to ensure compliance with environmental protection requirements.

[0119] The specific steps of the macroporous resin adsorption method are as follows:

[0120] 1. Resin Pretreatment

[0121] Cleaning: Use appropriate solvents (such as ethanol, water, etc.) to clean the resin to remove impurities and residues from the resin surface.

[0122] Drying: The cleaned resin is dried to remove its internal moisture.

[0123] Activation: Depending on the type of resin and application requirements, activation treatment may be necessary to improve its adsorption performance.

[0124] 2. Resin Filling

[0125] Choose a suitable adsorption column and ensure that its material, size and shape meet the experimental or production requirements.

[0126] The pretreated resin is uniformly packed into the adsorption column, taking care to avoid voids or blockages in the resin layer.

[0127] After filling, rinse the adsorption column with a suitable solvent (such as ethanol, water, etc.) to remove residual impurities and air bubbles.

[0128] Three: Adsorption Operation

[0129] The concentrated extract to be processed is passed through an adsorption column, so that the target components are adsorbed by the resin.

[0130] During the adsorption process, parameters such as flow rate, temperature, and concentration can be adjusted as needed to optimize the adsorption effect.

[0131] Monitor the quality of the effluent, and stop the adsorption operation when the content of the target component in the effluent reaches the predetermined standard.

[0132] IV: Washing and Dehydration Procedures

[0133] Once the resin is saturated with adsorption, the target component is eluted from the resin using an appropriate eluent.

[0134] The choice of eluent should be determined based on the properties of the target component to ensure the effectiveness and selectivity of the elution process.

[0135] Control the flow rate and amount of eluent to avoid waste and time costs.

[0136] 5. Resin Regeneration

[0137] The eluted resin needs to be regenerated to restore its adsorption performance.

[0138] Regeneration methods include chemical regeneration (such as treatment with acid or alkali solutions) and thermal regeneration (such as heat treatment).

[0139] Choose the appropriate regeneration method based on the type of resin and the actual situation, and follow the corresponding operating procedures.

[0140] VI: Collection and Processing

[0141] The eluted target components are collected and then subjected to subsequent analysis or purification procedures.

[0142] Waste liquid generated during adsorption and elution processes is classified and treated to ensure compliance with environmental protection requirements.

[0143] The regenerated resin may be reused or stored as needed.

[0144] The specific steps of the membrane separation method are as follows:

[0145] I. Membrane Selection

[0146] The appropriate separation membrane is selected based on factors such as the properties of the liquid to be treated, separation requirements, and economic costs. Common separation membranes include reverse osmosis membranes, nanofiltration membranes, ultrafiltration membranes, and microfiltration membranes, which have different pore sizes and retention characteristics and are suitable for different separation scenarios.

[0147] II. Pretreatment

[0148] Before membrane separation, the raw water or the liquid to be treated usually needs to be pretreated to remove large particulate impurities such as suspended solids and colloids, preventing membrane clogging. Pretreatment steps may include coagulation, sedimentation, and filtration. These steps can effectively reduce the turbidity and suspended solids content in the water, improving the effectiveness and efficiency of membrane separation.

[0149] III. Membrane Operation

[0150] Membrane module installation: Install the selected separation membrane into the membrane module and ensure the membrane module's sealing and stability.

[0151] System commissioning: Before starting the membrane separation system, perform system commissioning and checks to ensure that all parameters (such as pressure, flow rate, temperature, etc.) are within the normal range.

[0152] Membrane separation process: Pretreated water is passed through a membrane module. Utilizing the selective permeability of the membrane, specific components in the water (such as fluoride ions, organic matter, and microorganisms) are retained on one side of the membrane, while water molecules and other small molecules pass through. This process can be carried out at room temperature and does not require a phase change.

[0153] Monitoring and Adjustment: During membrane separation, it is necessary to monitor the system's operating status in real time, including indicators such as pressure, flow rate, and rejection rate. When abnormalities are detected, operating parameters need to be adjusted promptly or troubleshooting should be performed.

[0154] IV. Post-processing

[0155] Collection and treatment: The effluent after membrane separation is collected and further treated as needed, such as disinfection and pH adjustment, to meet water quality requirements.

[0156] Membrane cleaning and regeneration: During membrane separation, contaminants or impurities may accumulate on the membrane surface, leading to a decline in membrane performance. Therefore, regular cleaning and regeneration of the membrane are necessary to restore its separation performance. Cleaning methods include physical cleaning (such as backwashing, air washing, etc.) and chemical cleaning (such as using acid and alkali solutions, oxidants, etc.).

[0157] System maintenance: Regular maintenance and inspection of the membrane separation system are necessary, including checking the membrane module's seals and cleaning the system's piping and filters. These measures ensure stable system operation and extend its service life.

[0158] V. Precautions

[0159] During membrane separation, it is necessary to strictly control operating parameters such as pressure, flow rate, and temperature to avoid damaging the membrane or affecting the separation effect.

[0160] The choice of membrane should be determined based on the properties of the liquid to be treated and the separation requirements to ensure separation efficiency and cost-effectiveness.

[0161] Pretreatment steps are crucial for improving membrane separation efficiency and extending membrane lifespan, and therefore should be given due attention.

[0162] In the post-treatment process, the effluent needs to be treated to meet water quality requirements, and the membrane needs to be cleaned and regenerated regularly to maintain its performance.

[0163] In S6, drying and packaging refer to the post-processing stage of the extraction process, where the purified extract is dried to transform the liquid extract into a solid form. This not only facilitates subsequent processing and storage but also effectively reduces the product's volume and weight. Advanced drying technologies, such as spray drying and vacuum drying, ensure that the extract retains its original active ingredients and quality during the drying process. Subsequently, the resulting solid extract is carefully packaged using high-performance, moisture-proof, oxidation-proof, and light-protected materials to ensure its stability and safety during long-term storage and transportation, providing customers with high-quality products.

[0164] S7 refers to quality inspection and evaluation. In the extract production process, quality inspection is a crucial step to ensure quality standards are met. It comprehensively covers core indicators such as component content, purity, and safety. Utilizing advanced technology and equipment, it accurately measures the content of each component, assesses purity, and identifies potential safety risks. Following quality inspection, the extract's quality and efficacy are comprehensively evaluated based on the results, ensuring it meets industry standards and customer needs, and guaranteeing effective practical applications. Through this rigorous series of inspection and evaluation processes, we are committed to providing high-quality, safe, and reliable extract products, fully meeting the diverse needs of research, production, and medical fields, and ensuring customers receive optimal solutions.

[0165] Among them, the medicinal and edible extracts that repair damaged pancreatic islet cells based on the above methods can be applied to drug development, functional foods, health products, and medical research.

[0166] Drug Development: These extracts can serve as active ingredients in drugs to treat diseases such as diabetes caused by damage to pancreatic islet cells. By restoring the normal function of pancreatic islet cells, these extracts help lower blood sugar levels and improve the patient's health.

[0167] Functional foods: Adding extracts from both food and medicine sources to functional foods can create products that lower blood sugar and repair pancreatic islet cells. These foods are suitable for people with diabetes or those who need to prevent diabetes, helping to control blood sugar levels and improve quality of life.

[0168] Health supplements: Extracts derived from both food and medicine can also be used as ingredients in health supplements to enhance immunity and regulate blood sugar. These supplements are suitable for a wide range of people and help prevent disease and maintain health.

[0169] Medical Research: In the field of medical research, these extracts can serve as research tools to explore the mechanisms of pancreatic islet cell damage and to investigate new treatment methods. In-depth research into the mechanisms of action of these extracts can provide strong support for the development of more effective treatments and drugs.

[0170] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0171] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A medicinal and edible extract for repairing damaged pancreatic islet cells, characterized in that: This homologous extract contains the following ingredients in parts by weight: Panax notoginseng 15-35g, Cistanche deserticola 15-60g, Cornus officinalis 12-30g, Lonicera japonica 20-50g, Allium tuberosum 30-60g, Dendrobium officinale 25-60g, Morus alba leaf 25-60g, Eucommia ulmoides leaf 15-50g, Chlorella vulgaris 15-50g, Ginseng 15-60g, Bitter melon 20-50g, Saffron 15-50g, Citrus reticulata peel 15-50g, Gamma-aminobutyric acid 9-30g, Bee pollen 20-60g, Corn silk 15-60g, Arabinose 15-50g, Pueraria lobata 25-60g, Celery seed 20-60g, Powder 10-20g, Capsules 15-25g, Oral liquid 10-20g, Compressed candy 5-10g.

2. Preparation of a medicinal and edible extract for repairing damaged pancreatic islet cells, characterized in that: The specific steps for this preparation are as follows: S1: Raw material selection and pretreatment: Select raw materials carefully, clean them to remove impurities and dry them to ensure purity and suitability for extraction; S2: Grinding and Mixing: Finely grind and dry raw materials, and mix them in precise proportions to ensure a balanced composition of the extract. S3: Preparation of extraction solvent: Select a suitable solvent, prepare it according to the ratio, and ensure its purity and suitability; S4: Extraction and Concentration: Extraction with mixed raw materials using solvent, selecting reflux extraction or percolation extraction methods; concentrate the extract and remove the solvent. S5: Purification and Separation: Purify the extract to remove impurities and ensure the purity of the components. S6: Drying and Packaging: Drying to obtain a solid, packaging to ensure safety: processing, storage and transportation stability of the purified extract; S7: Quality Inspection and Evaluation: Quality inspection evaluates the effectiveness to ensure that the extract meets the standards and that safety and purity are guaranteed.

3. The preparation method of a medicinal and edible extract for repairing damaged pancreatic islet cells according to claim 1, characterized in that: The raw material selection and pretreatment in S1 refers to the selection of 35g of Panax notoginseng, 60g of Cistanche deserticola, 30g of Cornus officinalis, 50g of Lonicera japonica, 60g of Allium tuberosum, 60g of Dendrobium officinale, 60g of Morus alba leaves, 50g of Eucommia ulmoides leaves, 50g of Chlorella vulgaris, 60g of Panax ginseng, 50g of bitter melon, 50g of saffron, 50g of Citrus reticulata peel, 30g of γ-aminobutyric acid, 60g of bee pollen, 60g of corn silk, 50g of arabinose, 60g of Pueraria lobata, 60g of celery seed, 15g of powder, 20g of capsules, 20g of oral liquid, and 7.5g of compressed candy, followed by cleaning, impurity removal, and drying pretreatment to ensure the purity and suitability of the raw materials.

4. The preparation method of a medicinal and edible extract for repairing damaged pancreatic islet cells according to claim 1, characterized in that: The specific steps of crushing and mixing in S2 are as follows: Step 1: Fine grinding: Place each dried raw material into a grinder separately and adjust to the appropriate particle size to ensure that the raw material powder is fine and easy to dissolve, in preparation for the subsequent extraction process; Step 2: Precise Proportioning: Based on the usage range of each raw material, use precise measuring tools, such as an electronic scale, to weigh each raw material according to the predetermined ratio, ensuring that the proportion of each component is accurate; Step 3: Uniform Mixing: Finally, put the weighed raw material powders into a mixing container and mix them evenly using a stirrer or by hand to ensure that the proportions of each component in the extract are balanced, so as to improve the extraction effect.

5. The preparation method of a medicinal and edible extract for repairing damaged pancreatic islet cells according to claim 1, characterized in that: The solvent preparation in S3 refers to the need to fully consider the properties of the raw materials and the specific extraction requirements when selecting a solvent. For certain active ingredients, ethanol is the first choice due to its good solubility and penetrability; while for other ingredients, water or mixed solvents are more suitable. After selecting a solvent, it is necessary to prepare it strictly according to a certain ratio. At the same time, the purity of the solvent is ensured by means of filtration and distillation, and the optimal suitability is achieved by adjusting the temperature and pH parameters.

6. The preparation method of a medicinal and edible extract for repairing damaged pancreatic islet cells according to claim 1, characterized in that: The specific steps for extraction and concentration in S4 are as follows: Step 1: Mixing and Extraction: Combine the well-mixed raw materials with the selected solvent and use reflux extraction or percolation extraction methods to ensure that the effective components in the raw materials are fully dissolved in the solvent; Step 2: Extraction collection: After the extraction process is completed, carefully collect the extract containing the active ingredients, taking care to avoid loss and contamination; Step 3: Concentration treatment: The collected extract is concentrated by removing excess solvent through evaporation, and finally a concentrated extract containing high concentration of active ingredients is obtained.

7. The preparation method of a medicinal and edible extract for repairing damaged pancreatic islet cells according to claim 1, characterized in that: The specific steps for purification and separation in S5 are as follows: Step 1: Purification of the concentrated extract: Place the concentrated extract into a purification device and operate according to the specific requirements of either macroporous resin adsorption purification or membrane separation purification. When using macroporous resin adsorption, it is necessary to select a suitable resin type and adjust the appropriate pH value and concentration conditions so that the target component is adsorbed by the resin, while impurities are removed with the effluent. When using membrane separation technology, appropriate membrane modules and operating pressures are selected based on the pore size and molecular weight cutoff of the membrane, and impurities and unwanted components are removed through membrane filtration. Step 2: Separation of the purified extract: After the purification process, the resulting extract may still contain multiple components. In this case, it is necessary to select an appropriate separation method based on the properties of the target components and the separation requirements. Commonly used separation methods include solvent extraction, chromatographic separation, and electrophoresis. Appropriate separation conditions and operating parameters are selected based on the characteristics of the target component. The target component is separated from the purified extract through a separation step, resulting in a target component or component with higher purity. Step 3: Collection and Post-processing: After the separation step is completed, the target component or part should be collected promptly and properly stored. Based on subsequent application requirements, the collected target components or parts are further processed, such as drying, crystallization, and recrystallization, to improve their purity and stability. At the same time, the waste generated during the separation process is classified and treated to ensure compliance with environmental protection requirements.

8. The preparation method of a medicinal and edible extract for repairing damaged pancreatic islet cells according to claim 1, characterized in that: In step S6, drying and packaging refer to the post-processing stage of the extraction process, where the purified extract needs to be dried to transform the liquid extract into a solid form. Advanced drying technologies, such as spray drying and vacuum drying, ensure that the extract retains its original active ingredients and quality during the drying process. Subsequently, the resulting solid extract is carefully packaged using high-performance materials that are moisture-proof, oxidation-proof, and light-proof to ensure its stability and safety during long-term storage and transportation, providing customers with high-quality products.

9. The preparation method of a medicinal and edible extract for repairing damaged pancreatic islet cells according to claim 1, characterized in that: S7 refers to quality inspection and evaluation. In the production process of extracts, quality inspection is a key link to ensure that the quality meets the standards. It comprehensively covers the core indicators of component content, purity and safety. With the help of advanced technology and equipment, the content of each component is accurately measured, the purity is evaluated and potential safety risks are investigated. After quality inspection, the quality and effect of the extract are comprehensively evaluated based on the results to ensure that it meets industry standards and customer needs and guarantees the actual application effect.

10. The medicinal and edible extract for repairing damaged pancreatic islet cells according to claim 1 and its application, characterized in that: Based on the above methods, this food-grade extract for repairing damaged pancreatic islet cells can be applied to drug development, functional foods, health products, and medical research.

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