A traditional Chinese medicine compound for improving intestinal flora balance and a preparation method thereof

Abstract

This invention relates to the field of traditional Chinese medicine compound formulas, and discloses a traditional Chinese medicine compound formula for improving intestinal flora balance. The formula comprises the following components by weight: Astragalus membranaceus 10-15 parts; Atractylodes macrocephala 8-12 parts; Poria cocos 6-10 parts; Crataegus pinnatifida 7-10 parts; Coptis chinensis 3-5 parts; Citrus aurantium 6-9 parts; Cannabis sativa 3-5 parts; and Coix lacryma-jobi 8-12 parts. The Coptis chinensis, Citrus aurantium, and Cannabis sativa are pulverized before use, with a particle size of 10μm-20μm. A portion of the Crataegus pinnatifida, Citrus aurantium, and Cannabis sativa are fermented with probiotics at a temperature controlled at 35℃-40℃ for 24-48 hours. Through the fermentation of the traditional Chinese medicine components and probiotics, the number of beneficial bacteria such as Bifidobacteria and Lactobacillus in the intestine can be increased, while the growth of harmful bacteria such as Bacteroides and Enterococcus can be inhibited, thereby more effectively regulating the intestinal microecological environment and maintaining flora balance.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine compound technology, specifically to a traditional Chinese medicine compound for improving intestinal flora balance and its preparation method. Background Technology

[0002] With the development of modern medicine, the close relationship between gut microbiota health and overall human health has gradually gained attention. Studies have shown that the gut microbiota ecosystem is an important component in maintaining human immune function, metabolic balance, and nutrient absorption. Gut microbiota imbalance is closely related to the occurrence of various diseases, including gastrointestinal dysfunction, chronic enteritis, metabolic diseases, and even mental illnesses. Common intervention methods to improve gut microbiota imbalance mainly include probiotic supplementation, drug therapy, and dietary adjustments.

[0003] In existing technologies, probiotic supplements are widely used to regulate gut microbiota. However, the survival rate of probiotics and their colonization ability in the gastrointestinal tract are key issues regarding their efficacy. Due to the corrosive nature of stomach acid, many probiotics cannot effectively reach the intestines to exert their effects, thus significantly reducing therapeutic efficacy. Furthermore, probiotic supplementation alone cannot comprehensively address the complexity of gut microbiota imbalance, especially in suppressing the proliferation of harmful bacteria and inflammatory responses, where its effectiveness is limited.

[0004] On the other hand, traditional Chinese medicine (TCM) has significant advantages in regulating spleen and stomach function and improving intestinal health due to its multi-target and multi-level regulatory effects. However, the low bioavailability of TCM components is a major problem restricting its clinical application. Many effective components of TCM are easily decomposed or cannot be completely absorbed in the gastrointestinal tract, leading to unstable efficacy. At the same time, the release time and site of TCM are difficult to control, and its efficacy tends to decline rapidly, affecting its long-term regulatory effects.

[0005] Therefore, those skilled in the art provide a traditional Chinese medicine compound and its preparation method for improving the balance of intestinal flora, in order to solve the problems mentioned in the background art. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a traditional Chinese medicine compound and its preparation method for improving intestinal flora balance, solving the problems of poor probiotic effects and unstable efficacy in existing technologies.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a traditional Chinese medicine compound for improving the balance of intestinal flora, comprising the following components by weight:

[0008] Astragalus membranaceus 10-15 parts;

[0009] 8-12 parts of Atractylodes macrocephala;

[0010] Poria cocos 6-10 parts;

[0011] 7-10 servings of hawthorn;

[0012] Coptis chinensis 3-5 parts;

[0013] 6-9 parts of bitter orange peel;

[0014] 3-5 portions of hemp seeds;

[0015] 8-12 servings of Job's tears.

[0016] Preferably, the Coptis chinensis, Citrus aurantium, and Cannabis sativa seeds are pulverized before use, and the powder particle size is 10μm-20μm.

[0017] Preferably, some of the hawthorn, bitter orange peel, and hemp seed medicinal materials are fermented with probiotics, with the fermentation temperature controlled at 35℃-40℃ and the fermentation time at 24H-48H.

[0018] A method for preparing a traditional Chinese medicine compound for improving intestinal flora balance, comprising the following steps:

[0019] S1. Raw material processing: Wash, dry, and preliminarily pulverize Astragalus membranaceus, Atractylodes macrocephala, Poria cocos, Crataegus pinnatifida, Coptis chinensis, Citrus aurantium, Cannabis sativa seed, and Coix lacryma-jobi seed;

[0020] S2. Fermentation treatment: Add a portion of hawthorn, bitter orange peel and hemp seed to probiotic strains for fermentation;

[0021] S3, Ultrafine Grinding: Further grinding the pulverized medicinal materials into ultrafine powder;

[0022] S4. Coating treatment: Coptis chinensis and Citrus aurantium are coated with enteric coating, and hemp seed and Coix seed are coated with sustained-release coating.

[0023] S5. Mixing and compounding: Mix the treated medicinal materials and fermentation products evenly according to the formula ratio;

[0024] S6. Spray drying: The mixture is spray dried.

[0025] Preferably, in step S4, the thickness of the enteric coating is 5μm-10μm, and the thickness of the sustained-release coating is 10μm-15μm.

[0026] Preferably, the probiotics used in step S4 include, but are not limited to, Bifidobacteria and Lactobacillus.

[0027] Preferably, in step S6, the inlet air temperature of the spray drying is 120℃-130℃, and the outlet air temperature is 60℃-70℃.

[0028] Preferably, the particle size of the medicinal material after ultrafine pulverization in step S3 is 10μm-15μm.

[0029] Preferably, step S5 involves a second drying process after mixing, with a drying temperature of 40℃-50℃ and a drying time of 2H-4H.

[0030] Application of a traditional Chinese medicine compound for improving intestinal flora balance: The compound is used to regulate the imbalance between beneficial and harmful bacteria in the intestine, and to assist in the treatment of abdominal distension and loss of appetite caused by intestinal flora disorder.

[0031] This invention provides a traditional Chinese medicine compound for improving intestinal flora balance and its preparation method. It possesses the following characteristics:

[0032] Beneficial effects:

[0033] 1. This invention, through the fermentation treatment of traditional Chinese medicine ingredients and probiotics, can significantly increase the number of beneficial bacteria in the intestine, such as Bifidobacteria and Lactobacillus, while inhibiting the growth of harmful bacteria, such as Bacteroides and Enterococci. The fermentation technology enhances the biological activity of the medicinal materials, making the effects of probiotics more significant, thereby more effectively regulating the intestinal microecological environment and maintaining the balance of the gut microbiota.

[0034] 2. This invention uses enteric coating to treat Coptis chinensis and Citrus aurantium to ensure that the medicinal materials are not destroyed in the gastric acid environment and can be targeted to the intestines to exert the best efficacy; the sustained-release coating of Cannabis sativa seed and Coix seed ensures that the drug components can be continuously released in the intestines for a long time, thereby prolonging the drug action time, avoiding rapid decline of efficacy, and improving the overall sustainability of the therapeutic effect. Attached Figure Description

[0035] Figure 1 This is a flowchart of the preparation method of the present invention. Detailed Implementation

[0036] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] Basic Implementation

[0038] Please see the appendix Figure 1 This invention provides a traditional Chinese medicine compound for improving the balance of intestinal flora, comprising the following components by weight: Astragalus membranaceus 10-15 parts; Atractylodes macrocephala 8-12 parts; Poria cocos 6-10 parts; Crataegus pinnatifida 7-10 parts; Coptis chinensis 3-5 parts; Citrus aurantium 6-9 parts; Cannabis sativa 3-5 parts; Coix lacryma-jobi 8-12 parts.

[0039] Coptis chinensis, Citrus aurantium, and Cannabis sativa seeds are pulverized before use, with a particle size of 10μm-20μm.

[0040] Some of the medicinal materials, such as hawthorn, bitter orange peel, and hemp seed, are fermented with probiotics. The fermentation temperature is controlled at 35℃-40℃, and the fermentation time is 24H-48H.

[0041] Specifically, the selection of each component is based on its efficacy in traditional Chinese medicine in regulating the spleen and stomach, removing dampness and strengthening the spleen, and promoting intestinal health. Furthermore, the synergistic effect of multiple components helps to further optimize the balance of the intestinal flora.

[0042] In the preparation of this compound, Coptis chinensis, Citrus aurantium, and Cannabis sativa seeds need to be pulverized before use, with the particle size controlled within the range of 10μm to 20μm. This refined processing effectively increases the surface area of ​​the medicinal materials, thereby improving the dissolution rate and bioavailability of their active ingredients and ensuring the full exertion of the medicinal effect.

[0043] Furthermore, this invention incorporates probiotic fermentation technology, where certain medicinal materials (including hawthorn, immature bitter orange, and hemp seed) are fermented under specific conditions. The fermentation temperature is controlled between 35°C and 40°C, and the fermentation time varies from 24 to 48 hours. This fermentation process aims to further decompose the active ingredients in the medicinal materials through the metabolic action of probiotics, making them easier for the intestines to absorb, and promoting the proliferation of probiotics in the intestines, thereby enhancing the effect of the compound formula in regulating the intestinal microecology. This innovative approach, combining modern fermentation technology with traditional Chinese medicine processes, helps to maximize the efficacy of the medicine, and the addition of probiotics provides stronger support for intestinal health, particularly playing a positive role in maintaining the diversity and stability of the gut microbiota.

[0044] This compound formula, through the rational combination of various Chinese medicinal herbs and probiotic fermentation technology, can effectively regulate the intestinal flora, improve digestive function, and thus promote overall health.

[0045] A method for preparing a traditional Chinese medicine compound to improve intestinal flora balance includes the following steps:

[0046] S1. Raw Material Processing: First, the Chinese medicinal herbs, including Astragalus membranaceus, Atractylodes macrocephala, Poria cocos, Crataegus pinnatifida, Coptis chinensis, Citrus aurantium, Cannabis sativa seeds, and Coix lacryma-jobi, are washed to remove impurities, and then dried. The dried herbs need to be pre-crushed to ensure a suitable particle size for subsequent processing. This step ensures the cleanliness of the raw materials and the uniformity of the initial processing, laying the foundation for subsequent processing steps.

[0047] S2. Fermentation Process: A portion of the pre-crushed hawthorn, immature bitter orange peel, and hemp seed are added to a probiotic strain for fermentation. The probiotics used in the fermentation include, but are not limited to, Bifidobacterium and lactic acid bacteria. Fermentation conditions are strictly controlled within a suitable temperature (35℃-40℃) and time (24H-48H). Through the metabolic action of probiotics, the polysaccharides and other active ingredients in the medicinal materials are pretreated, making them easier for the intestines to absorb. At the same time, the bioactivity of probiotics further enhances the efficacy of regulating the intestinal flora.

[0048] S3. Ultrafine Pulverization: After fermentation, the medicinal materials need to be further pulverized into ultrafine powder, with the particle size controlled within the range of 10μm to 15μm. Ultrafine pulverization can greatly increase the specific surface area of ​​the medicinal materials, thereby improving their dissolution rate and absorption rate, and ensuring the rapid exertion of the medicinal effect.

[0049] S4. Coating Treatment: In this step, specific coating treatments are applied based on the characteristics of different medicinal materials. Coptis chinensis and Citrus aurantium undergo enteric coating treatment, with the coating thickness controlled between 5μm and 10μm to ensure that their active ingredients can be released at specific locations in the intestine, avoiding the destruction of their activity by gastric acid. Cannabis sativa seed and Coix seed utilize sustained-release coating technology, with a coating thickness of 10μm to 15μm, to slowly release the active ingredients and ensure their sustained effect in the intestine.

[0050] S5. Mixing and Compounding: Thoroughly mix the processed medicinal materials and fermentation products according to the formula ratio to ensure uniform distribution of components. After mixing, perform a second drying process, controlling the drying temperature between 40℃ and 50℃ for 2 to 4 hours, to further remove moisture from the mixture and ensure product stability.

[0051] S6. Spray Drying: The final step is spray drying, with the inlet air temperature controlled at 120℃ to 130℃ and the outlet air temperature at 60℃ to 70℃. This process rapidly evaporates the moisture in the mixture, forming a uniform dry powder product, ensuring the stability of the ingredients and easy storage.

[0052] This preparation method combines various modern pharmaceutical processes with traditional Chinese medicinal materials, which not only improves the efficacy of the medicinal materials but also enhances their intestinal regulatory effects, making them more effective in improving the balance of intestinal flora.

[0053] Example 1: Astragalus-based compound

[0054] step:

[0055] Raw material processing: Weigh out 15 parts of Astragalus membranaceus, 8 parts of Atractylodes macrocephala, 6 parts of Poria cocos, 7 parts of Crataegus pinnatifida, 3 parts of Coptis chinensis, 6 parts of Citrus aurantium, 3 parts of Cannabis sativa seed, and 8 parts of Coix lacryma-jobi seed according to their weight. Wash, dry, and preliminarily pulverize each herb.

[0056] Fermentation process: Add some hawthorn, bitter orange peel, and hemp seeds to probiotics (Bifidobacterium and lactic acid bacteria) for fermentation. The fermentation temperature is controlled at 37℃ and the fermentation time is 36 hours.

[0057] Ultrafine grinding: All medicinal materials are treated with ultrafine grinding, and the particle size is controlled between 10μm and 12μm.

[0058] Coating treatment: Coptis chinensis and Citrus aurantium were enteric coated with a coating thickness of 5 μm, while Cannabis sativa seed and Coix seed were sustained-release coated with a thickness of 10 μm.

[0059] Mixing and compounding: The treated medicinal materials are mixed evenly according to the formula ratio, and the secondary drying temperature is 45℃ for 3 hours.

[0060] Spray drying: inlet air temperature 125℃, outlet air temperature 65℃.

[0061] Summarize:

[0062] Astragalus-based compound formulas have significant effects in replenishing qi and benefiting the middle jiao (spleen and stomach). Combined with fermentation and slow-release coating technology, they can gradually improve the balance of intestinal flora and have a good long-term regulatory effect.

[0063] Example 2: Atractylodes macrocephala-dominant compound

[0064] step:

[0065] Raw material processing: Weigh out 12 parts of Atractylodes macrocephala, 10 parts of Astragalus membranaceus, 10 parts of Poria cocos, 8 parts of Crataegus pinnatifida, 5 parts of Coptis chinensis, 8 parts of Citrus aurantium, 5 parts of Cannabis sativa seed, and 12 parts of Coix lacryma-jobi seed, and wash, dry and initially pulverize them.

[0066] Fermentation process: Add some hawthorn, bitter orange peel, and hemp seed to lactic acid bacteria for fermentation. The fermentation temperature is controlled at 38℃ and the fermentation time is 48 hours.

[0067] Ultrafine grinding: Grinding medicinal materials to a particle size of 13μm.

[0068] Coating treatment: Coptis chinensis and Citrus aurantium were enteric coated with a coating thickness of 10 μm, while Cannabis sativa seed and Coix seed were sustained-release coated with a coating thickness of 12 μm.

[0069] Mixed compounding: The medicinal materials are mixed according to the formula ratio and then dried at 50℃ for 4 hours.

[0070] Spray drying: inlet air temperature 130℃, outlet air temperature 70℃.

[0071] Summarize:

[0072] The Atractylodes macrocephala-based compound focuses on strengthening the spleen and replenishing qi, removing dampness and invigorating the stomach. It has a more prominent overall regulating effect on gastrointestinal function, and is especially suitable for people with spleen deficiency and dampness. It helps to improve indigestion, abdominal distension and other problems.

[0073] Example 3: Poria cocos-based compound formula

[0074] step:

[0075] Raw material processing: Weigh out 10 parts of Poria cocos, 12 parts of Astragalus membranaceus, 10 parts of Atractylodes macrocephala, 9 parts of Crataegus pinnatifida, 5 parts of Coptis chinensis, 9 parts of Citrus aurantium, 5 parts of Cannabis sativa seed, and 10 parts of Coix lacryma-jobi seed, and wash, dry and initially pulverize them.

[0076] Fermentation treatment: Add some hawthorn, bitter orange peel, and hemp seed to Bifidobacterium for fermentation. The fermentation temperature is controlled at 35℃ and the fermentation time is 24 hours.

[0077] Ultrafine grinding: All medicinal materials are treated with ultrafine grinding, and the particle size is controlled to 10μm.

[0078] Coating treatment: The enteric coating thickness of Coptis chinensis and Citrus aurantium was 6 μm, and the sustained-release coating thickness of Cannabis sativa seed and Coix seed was 15 μm.

[0079] Mixed formulation: Mix the medicinal materials according to the formula ratio, and dry them at 40℃ for 2 hours.

[0080] Spray drying: inlet air temperature 120℃, outlet air temperature 60℃.

[0081] Summarize:

[0082] The compound formula, with Poria cocos as the main component, has the effect of strengthening the spleen and eliminating dampness, and can effectively improve intestinal problems caused by water retention, such as diarrhea and abdominal distension. Its fermentation treatment and sustained-release technology further enhance the persistence of its efficacy.

[0083] Summary:

[0084] In the three embodiments, Astragalus membranaceus, Atractylodes macrocephala, and Poria cocos are the main medicinal materials, all of which have the effect of regulating the spleen and stomach and improving the intestinal flora. Astragalus membranaceus focuses on tonifying qi and is suitable for those with qi deficiency; Atractylodes macrocephala is more inclined to strengthen the spleen and remove dampness, making it more suitable for those with digestive system disorders; Poria cocos excels in removing dampness and is particularly effective for intestinal problems caused by water retention. Through fermentation and coating technologies, the delayed release and targeted release of the medicinal effects are achieved, further enhancing the stability and persistence of the medicinal effects.

[0085] Comparative experimental design:

[0086] Comparative Experiment 1: Dosage Comparison Experiment

[0087] Experimental group: divided into high-dose, medium-dose, and low-dose groups, and dose difference analysis was performed.

[0088] Parameters: Changes in the number of gut microbiota (Bifidobacteria, Lactobacilli, Bacteroides, Enterococci).

[0089] Results: The high-dose group showed the best effect, followed by the medium-dose group, while the low-dose group showed the weakest effect, but all showed significant improvement.

[0090] Comparative Experiment 2: Comparative Experiment of Fermentation Treatment

[0091] Experimental group: The fermentation treatment group was compared with the unfermented group to observe the effect of fermentation treatment on the intestinal flora.

[0092] Parameters: number of beneficial bacteria, changes in intestinal pH.

[0093] Results: The number of beneficial bacteria in the fermentation treatment group increased significantly, and the pH value remained stable within the healthy range.

[0094] Comparative Experiment 3: Comparative Experiment of Coating Technology

[0095] Experimental groups: enteric-coated group, sustained-release coated group, and uncoated group.

[0096] Parameters: Drug release and effects in different segments of the intestine.

[0097] Results: The enteric coating group and the sustained-release coating group showed more precise release in specific areas of the intestine, which helped improve efficacy, and the sustained-release coating group had a longer duration of effect.

[0098] Experimental data table:

[0099]

[0100]

[0101] These experimental data show that the compound has a good effect on improving the balance of intestinal flora, especially in terms of increasing the number of probiotics and maintaining intestinal health.

[0102] To more comprehensively verify the effects of this traditional Chinese medicine compound on improving gut microbiota balance, we conducted a more detailed mouse experiment involving multiple experimental groups, and monitored changes in gut microbiota, gut health, and overall health status at different time points. The experimental content included: experimental design, experimental group setup, experimental procedures, measurement indicators, data analysis, and summary of experimental results.

[0103] Experimental Design:

[0104] Experimental animals: Healthy 6-week-old male C57BL / 6 mice, weighing between 18-22g, were selected. All mice were acclimatized to the environment for one week under the following conditions: temperature 22℃±2℃, humidity 50%±5%, and a 12-hour light-dark cycle.

[0105] Experimental grouping: Mice were randomly divided into 5 experimental groups, with 15 mice in each group, as follows:

[0106] High-dose group: 200 mg / kg of traditional Chinese medicine compound was administered daily.

[0107] Medium-dose group: 100 mg / kg of traditional Chinese medicine compound was given daily.

[0108] Low-dose group: 50 mg / kg of traditional Chinese medicine compound was given daily.

[0109] Positive control group: Administered commercially available probiotic preparations daily (dose of 100 mg / kg).

[0110] Control group: Administered the same amount of physiological saline daily.

[0111] The experiment lasted for 30 days, with medication administered daily via gavage.

[0112] Experimental steps:

[0113] Adaptation period: Mice were allowed to adapt to the environment for one week before the experiment to ensure their condition remained stable during the experiment.

[0114] Administration: The experiment lasted for 30 days, and the compound or control was administered by gavage at a fixed time each day (9:00 a.m.) to ensure that the mice in each group received the same compound or control.

[0115] Fecal collection: Fresh fecal samples were collected from mice on days 7, 15, and 30 to analyze the gut microbiota structure.

[0116] Blood collection: At the end of the experiment, blood was collected from all mice to test inflammatory factors, immune indicators, etc.

[0117] Tissue sampling: After the experiment, the mice were euthanized and their small and large intestine tissues were collected for histological analysis to observe the health status of the intestinal mucosa.

[0118] Measurement indicators:

[0119] Gut microbiota analysis: 16S rRNA gene sequencing technology was used to analyze the bacterial community in feces, focusing on changes in gut microbiota such as Bifidobacteria, Lactobacillus, Bacteroides, and Enterococcus.

[0120] Serum immune indicators: The levels of inflammatory factors such as IL-6, TNF-α, and IFN-γ in mouse serum were detected to assess the effect of the compound on the systemic inflammatory state.

[0121] Histological analysis: Mouse intestinal tissue was analyzed by HE staining to observe intestinal villus length, intestinal wall thickness and mucosal integrity, and to evaluate the effect of the compound on intestinal health.

[0122] Weight change: Weight changes in mice were recorded to assess the effects of the compound on overall health and nutrient absorption in mice.

[0123] Experimental data and results analysis:

[0124] 1. Gut microbiota analysis

[0125] Fecal samples were tested on days 7, 15, and 30, primarily focusing on changes in the abundance of probiotics (such as Bifidobacteria and Lactobacilli) and potentially harmful bacteria (such as Bacteroides and Enterococci). The following are the results of the gut microbiota testing:

[0126]

[0127]

[0128] Analysis results: The number of probiotics in the intestines of mice in the high-dose and medium-dose groups was significantly higher than that in the control group, especially the proliferation of Bifidobacteria and Lactobacillus. Compared with the positive control group, the traditional Chinese medicine compound group showed a better probiotic regulatory effect, while reducing the number of Bacteroides and Enterococci, indicating that the compound can effectively promote the reproduction of probiotics in the intestine and inhibit the growth of harmful bacteria.

[0129] 2. Serum immune markers

[0130] After the experiment, the levels of inflammatory factors in the serum of mice in each group were measured. The following are the results of the main indicators (unit: pg / ml):

[0131] High-dose group 15.2 21.4 12.7 medium dose group 18.3 24.6 14.5 low-dose group 22.5 28.1 17.2 Positive control group 20.1 26.3 15.4 control group 29.7 33.8 23.6

[0132] Analysis results: The levels of inflammatory factors in all dosage groups of the traditional Chinese medicine compound were significantly lower than those in the control group, especially in the high-dose group, indicating that the compound has a strong anti-inflammatory effect. The traditional Chinese medicine compound reduces the release of systemic inflammatory factors by regulating the intestinal flora and inhibiting intestinal inflammation.

[0133] 3. Histological analysis

[0134] Changes in mouse intestinal tissue structure were detected by HE staining. The following are the measurement data of small and large intestinal villus length (unit: μm) and intestinal wall thickness (unit: μm) in different groups:

[0135]

[0136]

[0137] Analysis results: The mice in the high-dose and medium-dose groups had significantly longer intestinal villi and thicker intestinal walls, indicating that the traditional Chinese medicine compound can significantly improve intestinal health and increase nutrient absorption.

[0138] 4. Weight changes

[0139] During the experimental period, the mice's body weight changed as follows:

[0140]

[0141] Analysis results: The mice in the high-dose and medium-dose groups showed significant weight gain, indicating that the compound has a clear advantage in promoting nutrient absorption and improving overall health.

[0142] Summary of experimental results:

[0143] The results of the mouse experiments show that:

[0144] Gut microbiota balance: The number of probiotics increased significantly in the high-dose and medium-dose groups, the gut microbiota structure was effectively optimized, and the growth of harmful bacteria was inhibited.

[0145] Anti-inflammatory effect: The compound significantly reduced the level of inflammatory factors in mouse serum, indicating that the traditional Chinese medicine compound has a significant inhibitory effect on intestinal and systemic inflammation.

[0146] Gut health: The compound significantly improved the intestinal tissue health of mice, increased the length of villi in the small and large intestines, and improved the efficiency of nutrient absorption.

[0147] Overall health: Data on weight gain in mice indicate that this compound not only improves gut health but also has a positive effect on overall nutrient absorption and health status.

[0148] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A traditional Chinese medicine compound for improving intestinal flora balance, characterized in that, The following components are included in parts by weight: Astragalus membranaceus 10-15 parts; 8-12 parts of Atractylodes macrocephala; Poria cocos 6-10 parts; 7-10 servings of hawthorn; Coptis chinensis 3-5 parts; 6-9 parts of bitter orange peel; 3-5 portions of hemp seeds; 8-12 servings of Job's tears.

2. The traditional Chinese medicine compound formula for improving intestinal flora balance according to claim 1, characterized in that, The Coptis chinensis, Citrus aurantium, and Cannabis sativa seeds are pulverized before use, with a particle size of 10μm-20μm.

3. The traditional Chinese medicine compound formula for improving intestinal flora balance according to claim 1, characterized in that, Some of the medicinal materials, including hawthorn, bitter orange peel, and hemp seed, are fermented with probiotics at a temperature controlled between 35℃ and 40℃ for 24-48 hours.

4. A method for preparing a traditional Chinese medicine compound for improving intestinal flora balance, as described in any one of claims 1-3, characterized in that, Includes the following steps: S1. Raw material processing: Wash, dry, and preliminarily pulverize Astragalus membranaceus, Atractylodes macrocephala, Poria cocos, Crataegus pinnatifida, Coptis chinensis, Citrus aurantium, Cannabis sativa seed, and Coix lacryma-jobi seed; S2. Fermentation treatment: Add a portion of hawthorn, bitter orange peel and hemp seed to probiotic strains for fermentation; S3, Ultrafine Grinding: Further grinding the pulverized medicinal materials into ultrafine powder; S4. Coating treatment: Coptis chinensis and Citrus aurantium are coated with enteric coating, and hemp seed and Coix seed are coated with sustained-release coating. S5. Mixing and compounding: Mix the treated medicinal materials and fermentation products evenly according to the formula ratio; S6. Spray drying: The mixture is spray dried.

5. The method for preparing a traditional Chinese medicine compound for improving intestinal flora balance according to claim 4, characterized in that, In step S4, the thickness of the enteric coating is 5μm-10μm, and the thickness of the sustained-release coating is 10μm-15μm.

6. The method for preparing a traditional Chinese medicine compound for improving intestinal flora balance according to claim 4, characterized in that, The probiotics used in step S4 include, but are not limited to, Bifidobacteria and Lactobacillus.

7. The method for preparing a traditional Chinese medicine compound for improving intestinal flora balance according to claim 4, characterized in that, In step S6, the inlet air temperature for spray drying is 120℃-130℃, and the outlet air temperature is 60℃-70℃.

8. The method for preparing a traditional Chinese medicine compound for improving intestinal flora balance according to claim 4, characterized in that, The particle size of the medicinal material after ultrafine pulverization in step S3 is 10μm-15μm.

9. The method for preparing a traditional Chinese medicine compound for improving intestinal flora balance according to claim 4, characterized in that, Step S5 involves a second drying process after mixing, with a drying temperature of 40℃-50℃ and a drying time of 2H-4H.

10. The application of a traditional Chinese medicine compound for improving intestinal flora balance, as described in any one of claims 1-3, characterized in that, The compound is used to regulate the imbalance between beneficial and harmful bacteria in the intestines, and to assist in the treatment of abdominal distension and loss of appetite caused by intestinal flora disorder.

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