A traditional Chinese medicine composition for preventing and treating bacterial bovine respiratory disease, and a preparation method and application thereof

Abstract

The application discloses a traditional Chinese medicine composition for preventing and treating bovine respiratory disease and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following components: Huangqi, Baibu, Chaihu, Kushen, Jiegeng, Shigao, Yuxingcao, Banlangen and Gancao. The traditional Chinese medicine composition has good palatability for ruminants, low cost, fast fever reduction, and remarkable curative effect on bovine respiratory disease caused by bacteria. Moreover, the raw materials of the composition are natural and healthy, and the composition is suitable for use in modern animal husbandry and organic ranches.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine for livestock and poultry, specifically relating to a traditional Chinese medicine composition for preventing and treating bacterial bovine respiratory diseases, its preparation method, and its application. Background Technology

[0002] In intensive ranches, factors such as weaning, weather changes, humid environments, and frequent herd transfers easily lead to stress in calves and the spread of pathogens in the environment. This results in severe respiratory diseases in weaned calves, such as pneumonia caused by mixed or secondary infections of Pasteurella multocida (PM), Mansonia haplocalyx (MH), Klebsiella pneumoniae (KP), Mycoplasma bovis (MB), Bovine viral diarrhea virus (BVDV), and Bovine infectious bronchitis virus (IBRV). Bacterial infections are the most common, causing mass mortality of calves. Even with successful antibiotic treatment, some calves become stunted, reducing their production performance. Developing a green and safe traditional Chinese medicine product that can prevent and treat bacterial respiratory diseases in calves is an urgent task. Summary of the Invention

[0003] The first technical problem to be solved by the present invention is to provide a traditional Chinese medicine composition for the prevention and treatment of bacterial bovine respiratory diseases, so as to overcome the shortcomings of existing Western medicine antibiotics that easily lead to drug resistance and stunted growth, and are difficult to deal with bacterial respiratory infections in weaned calves in intensive pastures.

[0004] The second technical problem to be solved by the present invention is to provide a method for preparing the above-mentioned traditional Chinese medicine composition.

[0005] The third technical problem to be solved by the present invention is to provide the application of the above-mentioned traditional Chinese medicine composition.

[0006] To solve the above-mentioned technical problems, the present invention discloses the following technical solution:

[0007] A traditional Chinese medicine composition comprising the following components: Scutellaria baicalensis, Stemona japonica, Bupleurum chinense, Sophora flavescens, Platycodon grandiflorus, Gypsum fibrosum, Houttuynia cordata, Isatis indigotica, and Glycyrrhiza uralensis.

[0008] In some embodiments, the weight parts of each component are: 70-90 parts of Scutellaria baicalensis, 30-60 parts of Stemona japonica, 60-99 parts of Bupleurum chinense, 60-90 parts of Sophora flavescens, 60-90 parts of Platycodon grandiflorus, 40-70 parts of Gypsum fibrosum, 30-50 parts of Houttuynia cordata, 40-60 parts of Isatis indigotica, and 40-70 parts of Glycyrrhiza uralensis.

[0009] In some embodiments, the weight parts of each component are: 70-85 parts of Scutellaria baicalensis, 35-55 parts of Stemona japonica, 60-85 parts of Bupleurum chinense, 60-80 parts of Sophora flavescens, 60-85 parts of Platycodon grandiflorus, 40-60 parts of Gypsum fibrosum, 30-45 parts of Houttuynia cordata, 40-55 parts of Isatis indigotica, and 40-60 parts of Glycyrrhiza uralensis; preferably, the weight parts of each component are: 75-85 parts of Scutellaria baicalensis, 35-50 parts of Stemona japonica, and 40-60 parts of Bupleurum chinense. 65-80 parts, Sophora flavescens 60-75 parts, Platycodon grandiflorus 60-80 parts, Gypsum 45-60 parts, Houttuynia cordata 30-40 parts, Isatis indigotica 45-55 parts, Glycyrrhiza uralensis 40-60 parts; more preferably, the weight parts of each component are Scutellaria baicalensis 80 parts, Stemona japonica 40 parts, Bupleurum chinense 70 parts, Sophora flavescens 60 parts, Platycodon grandiflorus 70 parts, Gypsum 50 parts, Houttuynia cordata 40 parts, Isatis indigotica 50 parts, Glycyrrhiza uralensis 50 parts.

[0010] This invention also protects a traditional Chinese medicine preparation comprising the above-mentioned traditional Chinese medicine composition.

[0011] In some embodiments, the dosage form of the traditional Chinese medicine preparation is an oral dosage form; preferably, the oral dosage form includes oral liquid, granules, powder, pills, tablets or capsules; the oral dosage form is prepared by using the traditional Chinese medicine contained in the above formula as the active ingredient and adding commonly used excipients or auxiliary ingredients to prepare an oral dosage form.

[0012] This invention also protects a product for preventing and treating bovine bacterial respiratory diseases, the product comprising the above-mentioned traditional Chinese medicine composition.

[0013] The preparation method of the above-mentioned traditional Chinese medicine composition is also within the scope of protection of this invention: weigh each component according to the formula, pulverize it, and then perform countercurrent extraction, solid-liquid separation, concentration and drying to obtain the final product.

[0014] In some embodiments, the solvent for countercurrent extraction is water; and / or, the mass ratio of the solvent to the traditional Chinese medicine component is 10-30:1, preferably 20:1; and / or, the temperature of the countercurrent extraction is 75-95℃ (preferably 80℃), and the time is 1-3h, preferably 2.5h; and / or, the concentration is carried out using a double-effect concentrator to concentrate to a relative density of 1-1.5, preferably 1.2.

[0015] Thirdly, this invention discloses the application of the aforementioned traditional Chinese medicine composition or the aforementioned traditional Chinese medicine preparation in the preparation of products for the prevention and treatment of bovine bacterial respiratory diseases; the products are pharmaceuticals or feed additives.

[0016] Fourthly, the present invention discloses a product for preventing and treating bovine bacterial respiratory diseases; the product comprises the traditional Chinese medicine composition described in the first aspect above.

[0017] In the third and fourth aspects mentioned above, the traditional Chinese medicine composition can be fed directly, added to water, or added to feed.

[0018] The dosage of the traditional Chinese medicine composition is calculated at 0.25-0.5 g / kg body weight per day, for 5-7 consecutive days.

[0019] When the application is for the prevention of respiratory diseases in cattle, such as during weather changes or transportation stress, the herbal composition is fed directly to the cattle daily at a dose of 0.25-0.5 g / kg body weight, which can be taken in divided doses or once. For prevention, the dosage can be halved and used for 7 consecutive days.

[0020] The preferred dosage is 0.3 g / kg body weight.

[0021] The prevention and / or treatment described in this invention include prevention and / or treatment.

[0022] The pharmacological properties of each herbal component in the above-mentioned herbal composition are as follows: Scutellaria baicalensis: Properties and channels: bitter, cold; enters the lung, gallbladder, spleen, large intestine, and small intestine channels; functions and indications: clears heat and dries dampness, drains fire and detoxifies, stops bleeding, and calms the fetus; used for lung heat cough, high fever and thirst, damp-heat fullness, and clearing upper lung heat. Stemona japonica: Properties and channels: sweet, bitter, slightly warm; enters the lung channel; functions and indications: moistens the lungs and stops cough, kills parasites; used for new and chronic cough, whooping cough, and pulmonary tuberculosis cough. Bupleurum chinense: Properties and channels: pungent, bitter, slightly cold; enters the liver and gallbladder channels; functions and indications: disperses and reduces fever, soothes the liver and relieves depression, and raises yang qi; used for colds with fever, alternating chills and fever, chest and rib pain, and lung heat cough and asthma. Sophora flavescens: Properties and channels: bitter, cold; enters the heart, liver, stomach, large intestine, and bladder channels; functions and indications: clears heat and dries dampness, kills parasites, promotes diuresis; used for damp-heat diarrhea, jaundice with dark urine, eczema, carbuncles due to heat toxin, cough and asthma due to phlegm-heat, etc. Platycodon grandiflorus: Properties and channels: bitter, pungent, neutral; enters the lung channel; functions and indications: promotes lung function and eliminates phlegm, soothes the throat and drains pus; used for impaired lung function, cough and asthma with excessive phlegm, sore throat, lung abscess, coughing up purulent sputum, etc. Gypsum: Properties and channels: sweet, pungent, very cold; enters the lung and stomach channels; functions and indications: clears heat and drains fire, relieves irritability and quenches thirst, calcined gypsum astringes dampness and heals sores; used for high fever with thirst, cough and asthma due to lung heat. Houttuynia cordata: Properties and channels: pungent, slightly cold; enters the lung channel; functions and indications: clears heat and detoxifies, eliminates carbuncles and drains pus, promotes urination and relieves strangury; used for lung abscess with purulent sputum, cough due to lung heat, carbuncles due to heat toxin, and strangury due to damp heat. Isatis indigotica: Properties and channels: bitter, cold; enters the heart and stomach channels; functions and indications: clears heat and detoxifies, cools the blood, and soothes the throat; used for exogenous fever, early stage of febrile diseases, and sore throat. Licorice: Properties and channels: sweet, neutral; enters the heart, spleen, lung, and kidney channels; functions and indications: tonifies the middle energizer and replenishes qi, clears heat and detoxifies, eliminates phlegm and relieves cough, relieves spasms and pain; used for insufficient heart qi, cough with excessive phlegm, etc.

[0023] This invention is formulated according to the principles of syndrome differentiation and treatment in traditional Chinese veterinary medicine and has undergone multiple clinical trials. Scutellaria baicalensis and Platycodon grandiflorus are the principal herbs, clearing heat, drying dampness, and promoting lung function to resolve phlegm. Scutellaria baicalensis, in particular, clears the upper lung heat and is the main principal herb for bacterial lung heat cough. Stemona japonica, Bupleurum chinense, Sophora flavescens, and Gypsum fibrosum are the assistant herbs, moistening the lungs, relieving cough, clearing heat, and drying dampness. Houttuynia cordata and Isatis indigotica are the adjuvant herbs, clearing heat and detoxifying. Glycyrrhiza uralensis is the guiding herb, tonifying the middle energizer, replenishing qi, clearing heat, detoxifying, resolving phlegm, and relieving cough. This formula primarily targets lung heat cough and asthma accompanied by damp-heat constitution and various factors causing respiratory diseases. It also addresses symptoms such as fever caused by respiratory diseases by rationally combining various herbs in a synergistic manner, significantly improving clinical efficacy.

[0024] Beneficial effects:

[0025] (1) The traditional Chinese medicine composition provided by the present invention is effective for respiratory diseases of cattle caused by bacterial infection. It has the effects of clearing the lungs and draining pus, moistening the lungs and relieving cough and promoting diuresis and removing dampness. It is safe, has no side effects, and treats both the symptoms and the root cause. It is a traditional Chinese medicine composition specially developed for cattle with damp-heat constitution. It is more effective than traditional cough-relieving and phlegm-reducing traditional Chinese medicine formulas and has a stronger antibacterial effect in calves.

[0026] (2) The traditional Chinese medicine composition provided by the present invention has significant therapeutic effects. It can not only control the cough and wheezing symptoms of cattle, but also quickly reduce fever, relieve symptoms, and increase the feed intake and disease resistance of sick cattle.

[0027] (3) The traditional Chinese medicine composition provided by the present invention is safe, effective, low cost, fast antipyretic, efficient, palatable and easy to use, and is used for the prevention and treatment of bovine bacterial respiratory diseases.

[0028] (4) The traditional Chinese medicine composition provided by the present invention has good palatability for ruminants, and cattle can actively eat or drink, which reduces the difficulty and workload of administration.

[0029] (5) The traditional Chinese medicine composition provided by the present invention has antipyretic effect. When cattle have cough, wheezing, pneumonia, etc., it can control the symptoms of fever and other complications, and can relieve symptoms, increase the feed intake and disease resistance of sick cattle, improve the cure rate and reduce the mortality rate. Attached Figure Description

[0030] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments, and the advantages of the present invention in the above and / or other aspects will become clearer.

[0031] Figure 1 The cattle experiencing respiratory distress in Example 2.

[0032] Figure 2 The herd of cattle exhibiting respiratory coughing and wheezing in Example 2.

[0033] Figure 3 The herbal medicine water used to drink the cattle in Example 2. Detailed Implementation

[0034] The present invention can be better understood from the following embodiments. However, those skilled in the art will readily understand that the descriptions in the embodiments are for illustrative purposes only and should not, and will not, limit the invention as detailed in the claims.

[0035] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, and the reagents, methods and equipment used are conventional reagents, methods and equipment in this technical field.

[0036] Figures 1-3 These are schematic diagrams of cattle experiencing respiratory distress, a herd of cattle coughing and wheezing, and the herd drinking traditional Chinese medicine water, as shown in Example 2.

[0037] Example 1: Preparation of Traditional Chinese Medicine Compound

[0038] Formula: Scutellaria baicalensis 80 kg, Stemona japonica 40 kg, Bupleurum chinense 70 kg, Sophora flavescens 60 kg, Platycodon grandiflorus 70 kg, Gypsum fibrosum 50 kg, Houttuynia cordata 40 kg, Isatis indigotica 50 kg, Glycyrrhiza uralensis 50 kg.

[0039] Preparation method: Weigh the above-mentioned medicinal materials according to the weight ratio, screen and wash them; pulverize the medicinal materials to pass through a 20-mesh sieve; extract using dynamic countercurrent reflux extraction method, with drinking water as the extraction solvent, the mass ratio of solvent to raw material being 20:1, the heating temperature being 80℃, and the extraction time being 2.5 h; after filtration, concentrate the extract to a relative density of 1.2. The concentration method uses a double-effect concentrator, with the vacuum degree and evaporation temperature of each effect being: first effect -0.05 MPa, 90℃; second effect -0.08 MPa, 70℃. The extract is then spray-dried to obtain the final product.

[0040] Example 2

[0041] 1. Materials and Methods

[0042] 1.1 Experimental Design

[0043] Forty calves around two months old with respiratory diseases were selected for the experiment. They were treated with the traditional Chinese medicine compound prepared in Example 1. The dosage was 30 g / 100 kg / day, based on the calf's body weight. The whole herd was given the medicine in drinking water, three times a day, at 8:00, 12:00 and 17:00, for a total of 5 days.

[0044] 1.2 Selection of experimental animals

[0045] The trial plans to select 2-month-old calves diagnosed with bacterial respiratory disease (positive for bacterial PCR test) as subjects. According to the scoring table, the inclusion criteria are a total clinical symptom score of ≥5 points, or two or more clinical parameters with scores of 2 or 3 points.

[0046] Table 1. Clinical parameters and scoring criteria used for respiratory system clinical scoring.

[0047]

[0048] 1.3 Grouping and Treatment of Experimental Animals

[0049] Forty calves with pneumonia were selected based on clinical scoring criteria, and 20 healthy calves were selected as the healthy control group (Con group). The calves with pneumonia were randomly divided into a positive drug (Ma Xing Shi Gan Tang, recommended dose 50 g / head / day) treatment group (PC group) and a traditional Chinese medicine compound treatment group (QBQFS group, provided in Example 1), with 20 calves in each group.

[0050] Table 2 Experimental Grouping and Treatment Scheme

[0051]

[0052] 1.4 Clinical symptom score

[0053] After 5 consecutive days of administration of traditional Chinese medicine, bacterial respiratory disease scores in calves were recorded on days 0, 1, 2, 3, 4, and 5. Detailed observations were conducted on the affected calves, and the clinical symptoms and their progression were recorded for each group throughout the experiment. Clinical symptom scores were assessed before and after medication using a scoring sheet.

[0054] 1.5 Pathogen detection

[0055] Bovine nasal swabs were collected before and after the experiment, and the main pathogens were detected using multiplex PCR.

[0056] 1.6 Hematological testing

[0057] 1.6.1 Blood Collection and Processing

[0058] Bovine jugular vein blood was collected before and after treatment (day 0 and day 6): 3 mL was collected using an EDTA-K2 anticoagulated blood collection tube (purple cap) for routine blood tests; 5 mL was collected using a non-anticoagulated blood collection tube (red cap) for blood biochemistry and acute-phase protein tests. Anticoagulated blood samples were gently mixed and stored at 4°C, with testing completed within 24 hours; non-anticoagulated blood samples were allowed to stand at room temperature for 30 minutes, centrifuged at 3000 rpm for 10 minutes to separate serum, and stored at -20°C for later testing.

[0059] 1.6.2 Detection Indicators and Methods

[0060] A complete blood count (CBC) was performed using a fully automated hematology analyzer. The indicators included: white blood cell count (WBC), neutrophil count and percentage (NEUT# / %), lymphocyte count and percentage (LYMPH# / %), monocyte count and percentage (MONO# / %), red blood cell count (RBC), hemoglobin concentration (HGB), hematocrit (HCT), and platelet count (PLT).

[0061] Blood biochemistry tests were performed using a fully automated biochemical analyzer. The indicators included: total protein (TP), albumin (ALB), globulin (GLOB), albumin / globulin ratio (A / G), alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (CREA), and blood glucose (GLU).

[0062] Each calf was scored for respiratory disease according to the respiratory disease diagnostic scoring criteria, and a score of 0 was considered cured.

[0063] 2 Results

[0064] As shown in Tables 3-1, 3-2, and 4, the cure standard was defined as a clinical symptom score ≤2 points and a decrease in score ≥50% after treatment. The cure rates for the two groups are as follows: Traditional Chinese medicine treatment group: 18 out of 20 sick calves met the cure standard, with a cure rate of 90% (18 / 20); Positive drug group: 15 out of 20 sick calves met the cure standard, with a cure rate of 75% (15 / 20). The cure rate of the traditional Chinese medicine treatment group was slightly higher than that of the positive drug group. As shown in Tables 5-1 and 5-2, in the traditional Chinese medicine treatment group, all 20 sick calves tested negative for bacterial pathogens using PCR after treatment, while in the positive drug group, only 14 out of 20 sick calves tested negative for bacterial pathogens using PCR after treatment. The results indicate that the treatment effect of the traditional Chinese medicine group was significantly better than that of the positive drug group.

[0065] Table 3-1 Treatment scores of bovine respiratory diseases in the traditional Chinese medicine treatment group

[0066]

[0067] Note: "-" indicates that no record was made after the treatment was completed.

[0068] Table 3-2 Treatment scores for respiratory diseases in bovines in the positive drug group

[0069]

[0070] Note: "-" indicates that no record was made after the treatment was completed.

[0071] Table 4. Effects of Traditional Chinese Medicine Compound Powder on Improving Clinical Symptoms of Bovine Bacterial Respiratory Disease

[0072]

[0073] Table 5-1 Results of bovine respiratory pathogen detection in the herbal treatment group

[0074]

[0075] Note: PM Pasteurella multocida; MH Mansonia hemolyticus; MB Mycoplasma bovis; KP Klebsiella pneumoniae; BVDV Bovine viral diarrhea virus; IBRV Bovine infectious bronchitis virus.

[0076] Table 5-2 Results of respiratory pathogen detection in bovine groups tested positive for the drug.

[0077]

[0078] Note: PM Pasteurella multocida; MH Mansonia hemolyticus; MB Mycoplasma bovis; KP Klebsiella pneumoniae; BVDV Bovine viral diarrhea virus; IBRV Bovine infectious bronchitis virus.

[0079] Blood routine test results (Table 6) showed that, compared with the normal group, the levels of WBC, NEU, LYM, and EOS were all elevated in the diseased group before treatment with traditional Chinese medicine, indicating a significant inflammatory response in the calves. After treatment with traditional Chinese medicine, the levels of WBC, NEU, and EOS in the diseased group decreased significantly compared with before treatment and approached the levels of the normal group, suggesting that traditional Chinese medicine treatment can alleviate the inflammatory response in calves with bovine respiratory disease (BRD). The changes in erythrocyte-related indicators such as RBC, HCT, MCV, MCH, and MCHC were relatively small, indicating that BRD had no significant impact on the erythrocyte system in this experiment.

[0080] Table 6. Blood Routine Test Results

[0081]

[0082] Blood biochemistry results (Table 7) showed that, compared with the normal group, the levels of AST, LDH, UREA, and T-Bil-V were elevated in the diseased group before treatment with traditional Chinese medicine, suggesting that the calves may have some degree of tissue damage and metabolic abnormalities. After treatment with traditional Chinese medicine, the levels of AST, LDH, UREA, and T-Bil-V in the diseased group decreased compared with those in the BRD group, indicating that the traditional Chinese medicine formula could improve the tissue damage and metabolic disorders caused by BRD. ALT and CREA-S did not change significantly among the three groups, suggesting that some indicators related to liver and kidney function were not significantly affected. TP and CA decreased after treatment, which may be related to nutritional status, feed intake, or individual differences during the disease recovery period.

[0083] Table 7 Blood Biochemistry Test Results

[0084]

[0085] 3. Conclusion

[0086] In summary, the traditional Chinese medicine compound of the present invention shows good therapeutic effect in treating bacterial respiratory diseases in calves, and the traditional Chinese medicine compound is safe and has no side effects.

[0087] This invention provides a traditional Chinese medicine composition for preventing and treating bacterial bovine respiratory diseases, its preparation method, and its application. Many methods and approaches exist for implementing this technical solution; the above description is merely a preferred embodiment of the invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this invention, and these improvements and modifications should also be considered within the scope of protection of this invention. All components not explicitly stated in this embodiment can be implemented using existing technologies.

Claims

1. A traditional Chinese medicine composition, characterized in that, It includes the following components: Scutellaria baicalensis, Stemona japonica, Bupleurum chinense, Sophora flavescens, Platycodon grandiflorus, Gypsum fibrosum, Houttuynia cordata, Isatis indigotica, and Glycyrrhiza uralensis.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, The weight proportions of each component are as follows: Scutellaria baicalensis 70-90 parts, Stemona japonica 30-60 parts, Bupleurum chinense 60-99 parts, Sophora flavescens 60-90 parts, Platycodon grandiflorus 60-90 parts, Gypsum fibrosum 40-70 parts, Houttuynia cordata 30-50 parts, Isatis indigotica 40-60 parts, and Glycyrrhiza uralensis 40-70 parts.

3. The traditional Chinese medicine composition according to claim 1, characterized in that, The weight proportions of each component are as follows: Scutellaria baicalensis 70-85 parts, Stemona japonica 35-55 parts, Bupleurum chinense 60-85 parts, Sophora flavescens 60-80 parts, Platycodon grandiflorus 60-85 parts, Gypsum fibrosum 40-60 parts, Houttuynia cordata 30-45 parts, Isatis indigotica 40-55 parts, and Glycyrrhiza uralensis 40-60 parts; preferably, the weight proportions of each component are: Scutellaria baicalensis 75-85 parts, Stemona japonica 35-50 parts, Bupleurum chinense 65-80 parts, and Sophora flavescens 60-85 parts. 0 parts, Sophora flavescens 60-75 parts, Platycodon grandiflorus 60-80 parts, Gypsum 45-60 parts, Houttuynia cordata 30-40 parts, Isatis indigotica 45-55 parts, Glycyrrhiza uralensis 40-60 parts; more preferably, the weight parts of each component are Scutellaria baicalensis 80 parts, Stemona japonica 40 parts, Bupleurum chinense 70 parts, Sophora flavescens 60 parts, Platycodon grandiflorus 70 parts, Gypsum 50 parts, Houttuynia cordata 40 parts, Isatis indigotica 50 parts, Glycyrrhiza uralensis 50 parts.

4. A traditional Chinese medicine preparation, characterized in that, Includes the traditional Chinese medicine composition according to any one of claims 1-3.

5. The traditional Chinese medicine preparation according to claim 4, characterized in that, The dosage form is an oral dosage form; preferably, the oral dosage form includes oral liquid, granules, powder, pills, tablets or capsules.

6. A product for preventing and treating bovine bacterial respiratory diseases, characterized in that, Includes the traditional Chinese medicine composition according to any one of claims 1-3.

7. A method for preparing the traditional Chinese medicine composition according to any one of claims 1-3, characterized in that, Weigh each component according to the formula, pulverize it, and then perform countercurrent extraction, solid-liquid separation, concentration and drying to obtain the final product.

8. The preparation method according to claim 7, characterized in that, The solvent for the countercurrent extraction is water; and / or, the mass ratio of the solvent to the traditional Chinese medicine component is 10~30:1; and / or, the temperature of the countercurrent extraction is 75~95℃, and the time is 1~3 h; and / or, the concentration is to concentrate to a relative density of 1~1.

5.

9. The use of the traditional Chinese medicine composition according to any one of claims 1-3, or the traditional Chinese medicine preparation according to any one of claims 4-5, in the preparation of products for the prevention and treatment of bovine bacterial respiratory diseases.

10. The application according to claim 9, characterized in that, The product in question is a pharmaceutical or feed additive.

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