A medicinal paste of the leaves of melia toosendan with the functions of removing blood stasis, promoting blood circulation and treating traumatic injury

Abstract

The present application relates to the technical field of medical ointment of the leaves of medical plant Melia toosendan, in particular to a medical ointment of the leaves of medical plant Melia toosendan with the functions of dispelling blood stasis, activating blood and treating contusions and injuries. The leaves of medical plant Melia toosendan are ultrasonically extracted by using choline chloride-lactic acid deep eutectic solvent (DES) and composite enzyme, and the enzyme is inactivated at 90-95 DEG C. The DES in the extract is not removed but directly used as an in-situ penetration enhancer. Meanwhile, the solid residues after extraction are sequentially subjected to alkaline treatment, bleaching and high-pressure homogenization, and then reconstructed in-situ into a dispersion of nanocellulose (CNC). The dispersion is used as a base to which sodium alginate is added, and then compounded with an extract containing borneol and total saponins of Panax notoginseng, and finally crosslinked by calcium ions to form an interpenetrating network hydrogel ointment. The present application breaks the traditional process of separation between extraction and molding, and the prepared ointment has excellent shear-thinning and thixotropy, avoiding secondary physical compression pain on the injured part during application, and realizing non-destructive penetration enhancement and intelligent gradient release of active ingredients.

Description

TECHNICAL FIELD

[0001] The present invention relates to the technical field of Turpinia arguta leaf ointments, and specifically, relates to a Turpinia arguta leaf ointment with functions of removing blood stasis, promoting blood circulation, and treating traumatic injuries. BACKGROUND ART

[0002] Traumatic injury is a common orthopedic condition in daily life and clinical practice, usually accompanied by local qi and blood stasis, acute tissue edema and severe pain. Traditional Chinese medicine external patches or ointments have the advantage of directly reaching the lesion in treating traumatic injuries and promoting blood circulation to remove blood stasis. As a traditional medicinal plant, Turpinia arguta is rich in flavonoids, triterpenoids and various volatile components in its leaves, and has good effects of promoting qi circulation to activate blood, detumescence and analgesia, and has been gradually applied in the development of external orthopedic drugs in recent years.

[0003] Most of the existing external Turpinia arguta ointments adopt the traditional mode of effective component extraction and separation and physical blending with an external matrix. In the extraction step, a water decoction method or an ethanol reflux extraction method is usually relied on. However, the water extraction method is difficult to effectively dissolve the water-insoluble liposoluble flavonoids and triterpenoid macromolecules in Turpinia arguta; although ethanol extraction can improve the dissolution rate to a certain extent, subsequent high-temperature concentration or reduced-pressure rotary evaporation must be performed to remove the organic solvent. This solvent removal process not only causes a large amount of thermal decomposition or escape of volatile components with auxiliary analgesic effects, but also easily promotes irreversible physical agglomeration of local high-concentration macromolecular active substances, thereby greatly reducing their bioavailability. In addition, a large amount of plant medicinal residues after extraction are usually directly discarded as solid waste, resulting in a serious waste of biomass resources.

[0004] In the steps of matrix compatibility and finished product preparation, the prior art usually directly mixes the concentrated Turpinia arguta extract with a conventional ointment matrix such as petrolatum, carbomer or macromolecular resin. However, this current method has some technical defects: first, the active components of Turpinia arguta have large molecular weights and complex structures, and have poor compatibility in conventional external matrices, resulting in an extremely low transdermal absorption rate. In order to promote the active components to penetrate the stratum corneum, existing conventional preparations often need to additionally add traditional chemical penetration enhancers such as azone and propylene glycol, which easily damages the barrier function of damaged skin and causes allergic irritation reactions such as erythema and itching at the affected part. Second, the conventional ointment matrix has a single rheological property, and often exhibits a relatively viscous or relatively hard physical state. The affected part of a traumatic injury is already in a highly sensitive inflammatory state with redness, swelling and pain. When a patient smears and spreads such a viscous ointment, a large mechanical friction force and pressing force must be applied, which easily causes strong secondary pain to the patient, and even aggravates local capillary rupture and microcirculation disorder due to excessive pressing.

[0005] Therefore, how to improve transdermal absorption rate while avoiding the loss of volatile components and the addition of irritating chemical penetration enhancers, while improving the rheological properties of the ointment to eliminate mechanical pain during application, and realizing the high-value utilization of all components of the plant matrix, is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0006] The purpose of this invention is to provide a *Syzygium aromaticum* leaf ointment with functions of removing blood stasis, promoting blood circulation, and treating traumatic injuries, in order to solve the technical problems mentioned in the background art, such as the easy loss of volatile components during the extraction of *Syzygium aromaticum* leaf, the low transdermal absorption rate of macromolecular active substances, the tendency of conventional ointment application to cause secondary mechanical pain at the site of traumatic injuries, and the direct disposal of medicinal residue.

[0007] To achieve the above objectives, the present invention provides a *Syzygium aromaticum* leaf ointment with functions of removing blood stasis, promoting blood circulation, and treating bruises and sprains, comprising the following components by weight: 100-150 parts of dried leaves of Panax notoginseng; 1500-2250 parts of eutectic solvent; 1.5-2.25 parts of compound enzyme; 5-15 parts of borneol; 10-30 parts of total saponins of Panax notoginseng; 30-50 parts of sodium alginate; 40-60 parts of cross-linking agent solution.

[0008] A method for preparing the above-mentioned *Syzygium aromaticum* leaf ointment, which has the functions of removing blood stasis, promoting blood circulation, and treating traumatic injuries, includes the following steps: S1. Eutectic solvent-compound enzyme directional extraction: The dried powder of Rhizoma Cynanchi 'Shanxiang' leaves is pulverized and passed through an 80-100 mesh sieve. It is added to the eutectic solvent at a material-to-liquid ratio of 1:15 (g / mL), followed by the addition of the compound enzyme. Ultrasonic-assisted enzymatic hydrolysis is performed for 40-60 minutes at 45℃-50℃ and pH 4.5-5.5. The ultrasonic frequency is 20-40kHz and the power is 200-300W. After extraction, the temperature is raised to 90℃-95℃ and kept at that temperature for 5 minutes to inactivate the enzyme. After cooling to 20℃-25℃, the mixture is centrifuged at 4000-5000r / min for 10-15 minutes. The supernatant (extract A rich in active ingredients) and the lower precipitate (solid residue B) are collected separately. The eutectic solvent is synthesized from choline chloride and lactate in a molar ratio of 1:2. The compound enzyme is composed of cellulase and pectinase in a molar mass ratio of 2:1.

[0009] S2. High-value reconstruction of solid medicinal residue: Alkali treatment: After washing the solid residue B obtained from S1 to neutral, add it to a sodium hydroxide solution with a mass concentration of 4%-6%, and stir at a constant temperature of 75℃-85℃ for 1.5-2 hours. Filter and wash the residue to neutral. Bleaching treatment: Add the alkali-treated filter residue to a mixture containing sodium chlorite and glacial acetic acid, adjust the pH to 3.5-4.5, react at 70℃-80℃ for 1-1.5 hours, filter and wash until neutral to obtain purified cellulose; Homogenization and dispersion: The purified cellulose was dispersed in deionized water to prepare a suspension with a mass fraction of 1%-2%, and homogenized under high pressure microjet 3-5 times at 60-80MPa to obtain a dispersion of Cyclocarya paliurus nanocellulose (CNC).

[0010] S3. Construction of self-assembled interpenetrating network ointment: Under conditions of 35℃-40℃, sodium alginate in the specified weight amount is slowly added to the CNC dispersion obtained in S2, and stirred at a uniform speed until completely dissolved; borneol and total saponins of Panax notoginseng are added to extract A obtained in S1, and dissolved by ultrasonication to form complex C; under shear emulsification conditions of 2000-3000 rpm, complex C is slowly added dropwise to the CNC dispersion containing sodium alginate; after the addition is complete, a calcium chloride solution with a mass concentration of 0.5%-1.0% is added dropwise to the system as a crosslinking agent solution, and stirring is continued for 10-15 minutes; the shear force is removed, and the system is allowed to cool naturally to 20℃-25℃ to undergo self-assembly, thus obtaining a thixotropic interpenetrating network hydrogel ointment.

[0011] Preferably, in S1, the method for synthesizing the eutectic solvent is as follows: choline chloride and lactic acid are placed in a stoppered flask and magnetically stirred under 80°C water bath conditions until a clear and transparent homogeneous liquid is formed, and then placed in a vacuum drying oven for degassing treatment before use.

[0012] Preferably, in S3, the crosslinking agent solution is a calcium chloride aqueous solution with a mass concentration of 0.5%.

[0013] Compared with the prior art, the beneficial effects of the present invention are as follows: This *Syzygium aromaticum* leaf ointment, which has the functions of removing blood stasis, promoting blood circulation, and treating bruises and sprains, uses a choline chloride-lactic acid eutectic solvent (DES) as the extraction solvent, eliminating the need for the high-temperature concentration and desolventizing process required by conventional ethanol extraction. This design not only fully preserves the heat-sensitive flavonoids and volatile components in *Syzygium aromaticum* leaves, but also, because the lactic acid and choline chloride retained in the system are hydrogen bond donors and acceptors, they can hydrate with the lipid bilayer of the stratum corneum, increasing the fluidity of intercellular lipids. This allows DES, as an in-situ transdermal penetration enhancer, to significantly improve the transdermal absorption rate of macromolecular flavonoids and total saponins from *Syzygium aromaticum* leaves without the addition of conventional irritating chemical penetration enhancers.

[0014] Secondly, this invention transforms waste medicinal residue into cellulose nanoparticles (CNC) in situ, replacing traditional hard ointment matrices such as petrolatum with a CNC dispersion. This CNC dispersion then cross-links with sodium alginate under the influence of calcium ions, forming a hydrogel framework with an interpenetrating polymer network (IPN). This network endows the ointment with specific shear-thinning (thixotropic) characteristics. When treating acute, swollen, and highly sensitive bruises and sprains, the ointment's viscosity drops sharply under the shear force of application, exhibiting an easily spreadable liquid state. After the shear force is removed, the network rapidly reconstructs and restores to a gel state through intermolecular hydrogen and ionic bonds. This rheological property avoids the mechanical pressing caused by high viscous resistance during traditional ointment application, reducing secondary physical irritation to the affected area.

[0015] Simultaneously, within the ointment system prepared by the method of this invention, the abundant active hydroxyl groups on the surface of the CNC framework form weak hydrogen bond interactions with the flavonoids of *Solanum nigrum* leaves and notoginseng saponins. After application, the local slight warming and inflammatory exudate at the site of bruises and sprains can gradually replace and destroy these weak hydrogen bonds. This allows the active ingredients free in the DES phase to penetrate rapidly and take effect, achieving rapid analgesia and swelling reduction in the initial stage; while the active substances bound to the CNC framework are released as the network slowly dissociates, prolonging the period of action of promoting blood circulation and removing blood stasis, and maintaining the effective drug concentration at the lesion site. Attached Figure Description

[0016] Figure 1 This is an overall flowchart of Embodiment 1 of the present invention. Detailed Implementation

[0017] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] Example 1: This example provides a *Syzygium aromaticum* leaf ointment with functions of removing blood stasis, promoting blood circulation, and treating bruises and sprains. Its raw material formula, by weight, includes: 100g of dried Panax notoginseng leaf powder, 1500g of eutectic solvent, 1.5g of compound enzyme, 10g of borneol, 20g of total Panax notoginseng saponins, 40g of sodium alginate, and 50g of 0.5% calcium chloride solution.

[0019] Its preparation method includes the following steps (such as...) Figure 1 (as shown) (1) Synthesis of eutectic solvent: Choline chloride and lactic acid were placed in a stoppered flask at a molar ratio of 1:2 and magnetically stirred in an 80°C water bath until a clear and transparent homogeneous liquid was formed. The liquid was then placed in a vacuum drying oven for degassing treatment before use.

[0020] (2) Targeted extraction: The dried powder of Rhodiola rosea leaves was pulverized and passed through an 80-mesh sieve. It was added to the above-mentioned eutectic solvent and a compound enzyme (the mass ratio of cellulase to pectinase was 2:1). The pH was adjusted to 5.0 and ultrasonic-assisted extraction was performed at 45℃ for 50 minutes (ultrasonic frequency 40kHz, power 250W). After extraction, the temperature was raised to 95℃ and kept warm for 5 minutes to inactivate the enzyme. After cooling to 20℃-25℃, it was centrifuged at 4500r / min for 15 minutes, and the upper extract A and the lower solid residue B were collected respectively.

[0021] (3) High-value reconstruction of solid medicinal residue: Solid medicinal residue B was washed until neutral, added to a 5% sodium hydroxide solution, stirred at 80℃ for 1.5 hours, filtered and washed until neutral; the filter residue was added to a mixture containing sodium chlorite and glacial acetic acid, the pH was adjusted to 4.0, reacted at 75℃ for 1 hour, filtered and washed until neutral to obtain purified cellulose. It was dispersed in deionized water to prepare a suspension with a mass fraction of 1.5%, and homogenized 4 times under high pressure microjets at 70MPa to obtain a dispersion of *Syzygium aromaticum* round leaf nanocellulose (CNC).

[0022] (4) Construction of interpenetrating network ointment: Sodium alginate was slowly added to the above CNC dispersion at 38°C and stirred at a constant speed until completely dissolved; borneol and total saponins of Panax notoginseng were added to extract A and dissolved by ultrasonication to form complex C; under shear emulsification at 2500 rpm, complex C was slowly added dropwise to the CNC dispersion containing sodium alginate; after the addition was complete, 0.5% calcium chloride solution was added dropwise and stirring was continued for 15 minutes; the shear force was removed and the mixture was allowed to cool naturally to 20°C-25°C to obtain the thixotropic interpenetrating network hydrogel ointment.

[0023] Example 2: The raw material formula of this example, by weight, includes: 100g of dried Panax notoginseng leaf powder, 1500g of eutectic solvent, 1.5g of compound enzyme, 5g of borneol, 10g of total Panax notoginseng saponins, 30g of sodium alginate, and 40g of 0.5% calcium chloride solution.

[0024] The preparation method is the same as in Example 1, except that the ultrasonic extraction temperature is 45℃, the pH is 4.5, the high-pressure homogenization pressure is 60MPa, and the homogenization is performed 3 times.

[0025] Example 3: The raw material formula of this example, by weight, includes: 150g of dried Panax notoginseng leaf powder, 2250g of eutectic solvent, 2.25g of compound enzyme, 15g of borneol, 30g of total Panax notoginseng saponins, 50g of sodium alginate, and 60g of 0.5% calcium chloride solution.

[0026] The preparation method is the same as in Example 1, except that the ultrasonic extraction temperature is 50℃, the pH is 5.5, the high-pressure homogenization pressure is 80MPa, and the homogenization is performed 5 times.

[0027] Comparative Example 1: The raw materials were the same as in Example 1. The difference in preparation method was that 100g of dried *Syzygium aromaticum* leaf powder was extracted twice by reflux with 70% ethanol (v / v). The extracts were combined and concentrated by rotary evaporation under reduced pressure to obtain *Syzygium aromaticum* leaf extract, and the residue was discarded. The extract, 10g of borneol, 20g of total saponins of Panax notoginseng, and appropriate amounts of petrolatum and lanolin matrix were physically melted and mixed under heating conditions, and then cooled to prepare a conventional petrolatum-based ointment.

[0028] Comparative Example 2: The extraction procedure was the same as S1 in Example 1, yielding extract A containing DES, but the residue B was discarded directly. Subsequent matrix preparation used Carbomer 940: Extract A, borneol, and a mixture of total Panax notoginseng saponins were added dropwise to a 2% Carbomer 940 hydrogel system neutralized with triethanolamine, and stirred until homogeneous to obtain the ointment.

[0029] Comparative Example 3: In the extraction step, pure water was used instead of the eutectic solvent (DES) for ultrasonic enzymatic extraction, followed by concentration of the extract. The treatment of the residue and the hydrogel construction steps were the same as S2 and S3 of Example 1.

[0030] To verify the beneficial effects of the ointment prepared according to the present invention, the ointments prepared in Examples 1-3 and Comparative Examples 1-3 were subjected to rheological properties, in vitro transdermal absorption and in vivo animal pharmacodynamic tests.

[0031] (1) Thixotropy and rheological tests: A rotational rheometer (test temperature 32℃, simulating skin surface temperature) was used to test the viscosity change (representing the degree of liquefaction during application) as the shear rate increased from 0.1s⁻¹ to 100s⁻¹, and the time required for the viscosity to recover to its initial state after the shear force was removed (representing the retention and reconstruction ability of the ointment at the affected area). Viscosity decrease rate = (initial viscosity - 100s⁻¹ viscosity) / initial viscosity × 100%.

[0032] (2) In vitro transdermal absorption test: The Franz diffusion cell method was used, with isolated nude mouse skin as the permeation barrier. Equal amounts of each group of ointments were placed in the supply cell, and the receiving cell was physiological saline containing 20% ​​ethanol (at a constant temperature of 37℃). After 24 hours, the receiving solution was collected, and the cumulative permeation rate (%) of total flavonoids (calculated as rutin) from the leaves of Rhizoma Sedum was determined by HPLC.

[0033] (3) Validation of rat traumatic injury model: Healthy SD rats were selected and a standard blunt force trauma injury was caused to the right hind limb (resulting in redness, swelling, and subcutaneous ecchymosis). The rats were randomly divided into groups, and each group was treated with the same amount of the corresponding ointment twice a day. The swelling reduction rate (%) of the affected limb on the 3rd day after administration and the average number of days required for complete dissipation of ecchymosis were recorded.

[0034] The test data are shown in Table 1.

[0035] Table 1: Results of rheological, transdermal, and animal model efficacy tests Example 1 94.2 8 68.4 82.5 4.2 Example 2 92.5 10 65.2 79.8 4.6 Example 3 95.1 7 69.1 84.1 4.0 Comparative Example 1 12.3 ＞60 15.6 35.2 8.5 Comparative Example 2 45.6 45 61.3 68.4 5.8 Comparative Example 3 93.8 9 24.5 45.6 7.1 Comparing Example 1 with Comparative Examples 1 and 2, it can be seen that the CNC-sodium alginate IPN gel used in this invention has extremely strong shear-thinning properties (viscosity reduction rate >92%) and can rapidly reconstruct and recover within 10 seconds. In clinical use, this manifests as melting upon application and solidifying immediately upon stopping, effectively avoiding the secondary mechanical pain caused by the high viscosity of Comparative Example 1 (Vaseline base), which requires forceful pressing.

[0036] Transdermal absorption enhancement effect analysis: Comparing Example 1 with Comparative Examples 1 and 3, it can be seen that the 24-hour cumulative permeation rate of flavonoids in Example 1 is more than 4 times that of the traditional alcohol-extracted petrolatum paste (Comparative Example 1) and nearly 2.8 times that of the pure water-extracted CNC system (Comparative Example 3). This proves that the DES solvent retained in the system has an extremely excellent transdermal absorption enhancement effect.

[0037] Pharmacodynamic analysis: The example showed superior results compared to the control groups in terms of swelling reduction and ecchymosis dissipation in an animal trauma model, confirming the scientific validity and advancement of the proposed solution's DES-enhanced rapid analgesia and swelling reduction, and CNC-based sustained-release for continuous blood circulation and stasis removal through a non-linear synergistic effect.

[0038] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a *Syzygium aromaticum* leaf ointment with functions of removing blood stasis, promoting blood circulation, and treating traumatic injuries, characterized in that... Includes the following steps: S1. Eutectic Solvent-Complex Enzyme Synergistic Directional Extraction: Pulverize the dried powder of Rhizoma Sinica var. sarcodactylis, add it to an eutectic solvent, then add the complex enzyme. Perform ultrasound-assisted enzymatic extraction at 45℃-50℃ and pH 4.5-5.

5. After extraction, raise the temperature to 90℃-95℃ and incubate for 5 minutes to inactivate the enzyme. After cooling to 20℃-25℃, centrifuge and collect the supernatant to obtain extract A and the lower precipitate to obtain solid residue B. The eutectic solvent is synthesized from choline chloride and lactate in a molar ratio of 1:2; the complex enzyme is composed of cellulase and pectinase in a molar mass ratio of 2:

1. S2. High-value reconstruction of solid medicinal residue: After washing the solid medicinal residue B obtained in S1, it is subjected to alkali treatment and bleaching treatment in sequence to obtain purified cellulose. The purified cellulose was then dispersed in deionized water and homogenized by high-pressure microfluidic jet to obtain a nanocellulose dispersion of Rhizoma Semperflorens var. mongolica. S3. Construction of self-assembled interpenetrating network ointment: Sodium alginate was added to the *Syzygium aromaticum* leaf nanocellulose dispersion obtained in S2 at 35℃-40℃ and stirred at a constant speed until completely dissolved; Borneol and total saponins of Panax notoginseng were added to extract A obtained in S1 and dissolved to form complex C; Under shear emulsification conditions, complex C was added dropwise to the *Syzygium aromaticum* leaf nanocellulose dispersion containing sodium alginate; After the addition was complete, calcium chloride solution was added dropwise as a crosslinking agent solution for crosslinking; The shear force was removed, and the system was allowed to cool naturally to 20℃-25℃ to undergo self-assembly, thus obtaining a thixotropic interpenetrating network hydrogel ointment.

2. The preparation method of the *Syzygium aromaticum* leaf ointment with functions of removing blood stasis, promoting blood circulation, and treating traumatic injuries according to claim 1, characterized in that, The raw materials for preparing this ointment, by weight, include the following components: 100-150 parts of dried leaves of Panax notoginseng; 1500-2250 parts of eutectic solvent; 1.5-2.25 parts of compound enzyme; 5-15 parts of borneol; 10-30 parts of total saponins of Panax notoginseng; 30-50 parts of sodium alginate; 40-60 parts of cross-linking agent solution.

3. The preparation method of the *Syzygium aromaticum* leaf ointment with functions of removing blood stasis, promoting blood circulation, and treating traumatic injuries as described in claim 1, characterized in that... In S1, the method for synthesizing the eutectic solvent is as follows: choline chloride and lactic acid are stirred in an 80°C water bath until a clear and transparent homogeneous liquid is formed, and then degassed for later use. In S1, the dried powder of Rhododendron simsii leaves is pulverized through an 80-100 mesh sieve; the ratio of dried Rhododendron simsii leaves to eutectic solvent is 1:15 g / mL; and the amount of compound enzyme added is 1.5% of the mass of the plant raw material.

4. The preparation method of the *Syzygium aromaticum* leaf ointment with functions of removing blood stasis, promoting blood circulation, and treating traumatic injuries according to claim 1, characterized in that... In step 1, the conditions for ultrasound-assisted enzymatic hydrolysis extraction are: temperature 45℃-50℃, pH value 4.5-5.5, extraction time 40-60 minutes, ultrasonic frequency 20-40kHz, and power 200-300W; the centrifugation conditions are: centrifugation at 4000-5000r / min for 10-15 minutes.

5. The preparation method of the *Syzygium aromaticum* leaf ointment with functions of removing blood stasis, promoting blood circulation, and treating traumatic injuries according to claim 1, characterized in that... The specific operation of S2 is as follows: Alkali treatment: After washing the solid residue B obtained from S1 to neutral, add it to a sodium hydroxide solution with a mass concentration of 4%-6%, and stir at a constant temperature of 75℃-85℃ for 1.5-2 hours. Filter and wash the residue to neutral. Bleaching treatment: Add the alkali-treated filter residue to a mixture containing sodium chlorite and glacial acetic acid, adjust the pH to 3.5-4.5, react at 70℃-80℃ for 1-1.5 hours, filter and wash until neutral to obtain purified cellulose; Homogenization and dispersion: The purified cellulose was dispersed in deionized water to prepare a suspension with a mass fraction of 1%-2%, and then subjected to high-pressure micro-jet homogenization 3-5 times under a pressure of 60-80MPa to obtain a dispersion of cellulose nanoparticles from the leaves of the Rhizoma Semperflorens.

6. The preparation method of the *Syzygium aromaticum* leaf ointment with functions of removing blood stasis, promoting blood circulation, and treating traumatic injuries according to claim 1, characterized in that... In step S3, the mass fraction of sodium alginate in the dissolved system is 1.5%-2%; the shear emulsification conditions are: rotation speed 2000-3000 rpm; the crosslinking agent solution is a calcium chloride aqueous solution with a mass concentration of 0.5%-1.0%, and stirring is continued for 10-15 minutes after the crosslinking agent solution is added dropwise.

7. A medicinal plaster containing *Syzygium aromaticum* leaf extract, characterized in that it has the functions of removing blood stasis, promoting blood circulation, and treating bruises and sprains. It is prepared by the preparation method according to any one of claims 1-6.

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