A cultivation microbial agent for promoting high yield of sugarcane and application thereof

Abstract

The application discloses a cultivation microbial agent for promoting high yield of sugarcane and application thereof, and belongs to the technical field of fertilizers. The cultivation microbial agent for promoting high yield of sugarcane comprises the following raw materials in parts by mass: a microbial compound 3-4 parts, sugarcane leaf fermentation 5-8 parts, oyster shell 2-3 parts, dolomite powder 1-2 parts, corn cob 10-12 parts, potato starch 5-6 parts, sugarcane residue 2-3 parts, beet 2-3 parts and carboxymethyl cellulose 2.5-3.5 parts. The cultivation microbial agent can promote secretion of sugarcane regulating hormones and absorption of nutrient elements, has functions of nitrogen fixation, phosphorus solubilization and potassium solubilization, and therefore can improve the cold resistance of sugarcane, guarantee normal growth of the sugarcane and improve the yield of the sugarcane.

Description

Technical Field

[0001] This invention relates to the field of fertilizer technology, and in particular to a cultivation microbial agent for promoting high sugarcane yield and its application. Background Technology

[0002] sugar cane( Sugar factories Sugarcane is a C4 crop with strong photosynthetic capacity. It is an annual or perennial, deep-rooted crop, distributed in major subtropical and tropical countries and regions. Sugarcane is a warm-loving, light-loving crop, suitable for planting in fertile soil, with ample sunshine and large temperature differences between winter and summer. It is the raw material for making sugar and can also be used to extract fuel ethanol as an energy alternative. It is distributed between 33°N and 30°S and is the world's most important sugar crop.

[0003] With the improvement of varieties, sugarcane planting areas have expanded to regions approaching 26° North latitude. However, in subtropical and temperate regions, sugarcane is often affected by low temperatures (chilling injury and frost damage). Chilling injury and frost damage not only seriously affect sugarcane yield and quality, but also affect the economic income of sugarcane farmers and the stable development of the sugar industry.

[0004] Currently, sugarcane frost protection measures mainly include promoting the application of cold-resistant varieties, improving cultivation management, and chemical regulation. While these methods can address sugarcane frost damage to some extent, cold-resistant sugarcane varieties often have lower yields and sugar content, and suffer from long breeding cycles, high investment, and slow returns. Improving cultivation management involves high labor and time costs and is limited by environmental and terrain conditions. Chemical regulation faces challenges such as difficulty in controlling spraying time, potential environmental pollution, and ineffectiveness in addressing root frost damage. Therefore, effectively solving the sugarcane frost damage problem and achieving high-yield and high-efficiency sugarcane production has become a pressing technical challenge for those skilled in the art. Summary of the Invention

[0005] The purpose of this invention is to provide a cultivation microbial agent that promotes high sugarcane yield and its application, so as to solve the problems existing in the prior art.

[0006] To achieve the above objectives, the present invention provides the following solution: One of the technical solutions of the present invention is a cultivation inoculant for promoting high sugarcane yield, comprising the following raw materials in parts by weight: 3-4 parts of microbial complex, 5-8 parts of sugarcane leaf fermentation, 2-3 parts of oyster shell, 1-2 parts of dolomite powder, 10-12 parts of corn cob, 5-6 parts of potato starch, 2-3 parts of sugarcane bagasse, 2-3 parts of sugar beet, and 2.5-3.5 parts of carboxymethyl cellulose; The microbial complex comprises the following components in parts by weight: 5-8 parts of Bacillus jellyii, 1-2 parts of Lactobacillus acidophilus, 3-4 parts of Rhodopseudomonas palustris, 2-3 parts of Candida utilis, 5-6 parts of Bacillus subtilis, and 4-5 parts of Bacillus megaterium.

[0007] The microbial complex of the present invention has the effects of improving soil fertility, promoting crop growth, inducing crop resistance, promoting soil microbial balance, improving soil physical and chemical properties, inhibiting harmful bacteria, and improving crop quality and yield.

[0008] Furthermore, the method for preparing the sugarcane leaf ferment includes the following steps: Sugarcane leaves are crushed, then honey and fermentation bacteria are added, mixed evenly, and then subjected to anaerobic fermentation to obtain the sugarcane leaf fermentation product.

[0009] Furthermore, the mass ratio of the sugarcane leaves, honey, and fermentation bacteria is 100:(1~2):(0.5~1).

[0010] Furthermore, the fermenting bacteria include Lactobacillus plantarum; The anaerobic fermentation is carried out at a temperature of 35-40℃ for 48-60 hours.

[0011] Fermented sugarcane leaves are rich in beneficial substances such as amino acids, vitamin B, and minerals (such as calcium, iron, and zinc). These substances not only provide nutrients for microorganisms and promote the secretion of plant growth-regulating hormones, but can also be absorbed by plants to improve their cold resistance.

[0012] Oyster shells and dolomite powder can release calcium ions under the action of microorganisms, while corn cobs can release xylitol. Xylitol can chelate calcium ions to form sugar alcohol calcium, which is easily absorbed by plants, thus increasing the calcium content in plants. Calcium is an important component of cell walls. It can combine with pectin to form stable calcium pectate, enhancing the thickness and toughness of cell walls. Under low-temperature conditions, thick cell walls can effectively resist cell contraction and rupture, reducing damage to tissues caused by freezing. Calcium can stabilize cell membrane structure, reduce its permeability at low temperatures, reduce the leakage of solutes from cells, and maintain normal metabolic functions. At the same time, calcium can regulate the activity of antioxidant enzymes, reducing free radical damage to the membrane system. Calcium can promote the accumulation of osmotic regulators such as soluble sugars and proline, increase cell sap concentration, lower the freezing point, and prevent cells from excessively losing water and freezing.

[0013] Potato starch, sugarcane bagasse, and sugar beets are rich in sugars, which can increase the sugar content in plants and improve their resistance to frost damage.

[0014] The second technical solution of the present invention: a method for preparing the above-mentioned cultivation inoculant for promoting high sugarcane yield, comprising the following steps: (1) Mix the microbial complex, oyster shell, dolomite powder and corn cob evenly, spray with starch aqueous solution, and granulate at low temperature to obtain microbial particles; (2) Mix sugarcane leaf fermentation, potato starch, sugarcane bagasse and beet, dry them, and then pulverize them to obtain mixed powder; (3) The mixed powder is coated on the surface of the microbial particles to obtain compound fertilizer particles; (4) Spray an aqueous solution of carboxymethyl cellulose onto the surface of the compound fertilizer granules, and dry them to obtain the cultivation agent that promotes high sugarcane yield.

[0015] The cultivation inoculant prepared using the method of this invention can continuously and stably release nutrients, while also enhancing microbial activity and significantly improving plant stress resistance and yield.

[0016] Further, in step (1), the concentration of the starch aqueous solution is 1.5~2 wt.%; In step (1), the particle size of the microbial particles is 1.5~2mm.

[0017] Furthermore, in step (2), the particle size of the mixed powder is 200~300 mesh; In step (4), the aqueous solution of carboxymethyl cellulose is prepared by mixing carboxymethyl cellulose and water in a mass ratio of (2.5~3.5):100.

[0018] Furthermore, in step (1), the temperature of the low-temperature granulation is 35~45℃; In step (4), the drying temperature is 40~45℃.

[0019] The third technical solution of the present invention: the application of the above-mentioned cultivation inoculant in sugarcane planting.

[0020] The fourth technical solution of the present invention: the application of the above-mentioned cultivation inoculant in the preparation of an antifreeze composition that improves the cold resistance of sugarcane.

[0021] The present invention discloses the following technical effects: The cultivation inoculant of this invention can promote the secretion of sugarcane regulatory hormones and the absorption of nutrients, and has functions such as nitrogen fixation, phosphorus solubilization, and potassium solubilization. Therefore, it can improve the sugarcane's ability to resist cold damage, ensure the normal growth of sugarcane, and increase the yield of sugarcane. Detailed Implementation

[0022] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0023] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0024] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0025] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0026] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0027] It should be noted that any aspects not described in detail in this invention are conventional practices in the field and are not the focus of this invention.

[0028] sugar cane( SugarSugarcane (spp. hybrid) belongs to the C4 crop of the Poaceae family. Native to tropical and subtropical regions, it is characterized by its preference for high temperatures and sunlight, high water requirements, high nutrient absorption, long growth cycle, and wide range of uses. Currently distributed between 33°N and 30°S, it is one of the world's most important sugar crops. Based on its uses, sugarcane can be divided into sugarcane, energy sugarcane, edible fruit sugarcane, feed sugarcane, fiber-specific sugarcane, and dual-purpose sugarcane. Among different types of sugarcane, sugarcane has the largest planting area. Due to different processing techniques, sugarcane can be used to produce different types of sugar products, such as raw sugar, brown sugar, white sugar, and rock sugar. Byproducts from sugar production, such as sugarcane leaves, bagasse, and waste honey, can be used as animal feed, biofuel, organic fertilizer, ethanol extraction, or fiber materials. With variety improvement, sugarcane planting areas have expanded to regions approaching 26°N latitude. However, in subtropical and temperate regions, sugarcane is often affected by low temperatures (chilling injury and frost damage). Cold damage and freezing damage not only seriously affect sugarcane yield and quality, but also affect the economic income of sugarcane farmers and the stable development of the sugar industry.

[0029] The impact of low temperature on sugarcane production (1) Impact on output Frost damage to leaves prevents photosynthesis, affecting the synthesis of organic matter. If the growing point of frost-damaged sugarcane dies, the plant cannot grow and can only sprout lateral buds. Frost damage can cause hollow stalks, resulting in a yield loss rate of up to 10%. In sugarcane where the entire stalk is damaged, the stalk deteriorates and loses weight; if not treated promptly, the sugar in the stalk will be converted into alcohol, leading to even greater yield loss.

[0030] (2) Affects the quantity and quality of seedlings in the next crop. When sugarcane stalks are severely subjected to frost stress, the growing point and lateral buds die, reducing both the quality and quantity of seed production, which seriously affects sugarcane farmers and the sugarcane industry.

[0031] (3) Affects the development of ratoon sugarcane plants When sugarcane plants are damaged by frost, the lateral buds at the base of the sugarcane stem die, resulting in a decrease in the number of ratoon plants, a low germination rate, delayed germination, poor growth, and a reduced yield of ratoon sugarcane the following year.

[0032] (4) Affects the sugar content and quality of raw sugarcane stalks For sugar companies, the most serious impact is the decline in the quality of raw sugarcane sugar content. Sugarcane damaged by frost can lose 5-10% of its sugar content, while also experiencing an increase in reducing sugar content, as well as an increase in the acidity and colloids in the juice, thus reducing the quality of the juice and making sugar production difficult.

[0033] Currently, measures for protecting sugarcane from cold and freezing mainly include promoting and applying cold-resistant varieties, improving cultivation management practices, and chemical regulation.

[0034] However, promoting and applying varieties with strong cold resistance presents the following problems: Most cold-resistant varieties often perform poorly in core economic traits such as yield and sugar content, or have other defects such as easy lodging and weak disease resistance, making it difficult to meet the dual requirements of "high quality + cold resistance" for commercial production; sugarcane hybrid breeding has a long cycle and the genetic mechanism of cold resistance is complex, requiring multiple generations of screening and multi-point trials, with large R&D investment and slow results, making it difficult to quickly respond to sudden climate cooling or new low-temperature disasters; Improving cultivation management practices has revealed the following problems: Cultivation and management measures (such as rational fertilization, mulching, hilling, irrigation and frost protection) have high labor and time costs (thick hilling in winter requires a lot of manpower; mulching requires purchasing mulch in advance, laying it manually, and recycling the residual mulch later, which increases planting costs and labor, and has a low cost-effectiveness in large-scale planting), and are limited by environment and terrain (irrigation and frost protection depend on water source conditions and cannot be implemented in arid areas; mulching on slopes is easily damaged by wind and rain, and hilling is easy to harden in heavy clay soil, which greatly reduces the effect).

[0035] Chemical regulation has the following problems: Chemical regulation enhances plant resistance by spraying plant growth regulators and antifreeze agents. However, antifreeze agents need to be sprayed 7-10 days before the onset of low temperatures to provide effective protection. If the optimal time is missed (e.g., due to a sudden drop in temperature), the spraying is essentially ineffective. Furthermore, a single spray is only effective for 15-20 days, requiring multiple applications during prolonged cold and damp weather, making the process cumbersome. Some chemical regulators, if used long-term or in excessive amounts, may accumulate in sugarcane plants, affecting product quality and safety. Additionally, these agents can seep into the soil or flow into water bodies with rainwater, potentially polluting the surrounding environment. Most spray-type antifreeze agents primarily act on the leaves and stems, offering little protection to the underground root system. Root damage caused by low temperatures is often a key reason for sugarcane wilting later in the season.

[0036] In the following examples, "parts" refers to "parts by weight".

[0037] In the specific embodiments of this invention, the following bacteria—Bacillus colloidis, Lactobacillus acidophilus, Rhodopseudomonas palustris, Candida utilis, Bacillus subtilis, and Bacillus megaterium—were all purchased from the China Industrial Microbiological Culture Collection Center. Specifically, the product number for Bacillus colloidis is CICC 21700; for Lactobacillus acidophilus, CICC 6094; for Rhodopseudomonas palustris, CICC 23812; for Candida utilis, CICC 31170; for Bacillus subtilis, CICC 10732; for Bacillus megaterium, CICC 23076; and for Lactobacillus plantarum, CICC 25125.

[0038] Example 1 A cultivation inoculant to promote high sugarcane yield: (1) The cultivation agent for promoting high sugarcane yield is composed of the following raw materials in parts by weight: 4 parts microbial complex, 6 parts sugarcane leaf fermentation, 3 parts oyster shell powder, 1 part dolomite powder, 12 parts corn cob powder, 5 parts potato starch, 2.5 parts sugarcane bagasse, 2 parts sugar beet and 3 parts carboxymethyl cellulose. The moisture content of corn cob powder is about 8%. The moisture content of sugarcane bagasse is about 15%; Beetroot refers to the fresh stems and leaves of the beet.

[0039] (2) Preparation of microbial complexes Bacillus jellyoides was activated and cultured in liquid culture medium recommended by the China Industrial Microbial Culture Collection Center, followed by fermentation culture, yielding an effective viable count of 1.0 × 10⁻⁶. 9 CFU / mL Bacillus jelly-like bacterial suspension; after filtering the Bacillus jelly-like bacterial suspension with filter paper, it was dried at low temperature (30℃) to obtain Bacillus jelly-like bacterial powder; Lactobacillus acidophilus was activated and cultured in liquid culture medium recommended by the China Industrial Microbial Culture Collection Center, followed by fermentation culture, yielding an effective viable count of 5.0 × 10⁻⁶. 8 Lactobacillus acidophilus bacterial solution (cfu / mL); after filtering the Lactobacillus acidophilus bacterial solution with filter paper, it was dried at low temperature (30℃) to obtain Lactobacillus acidophilus bacterial powder; Rhodopseudomonas palustris was activated and cultured in liquid culture medium recommended by the China Industrial Microbial Culture Collection Center, followed by fermentation culture, yielding an effective viable count of 8.0 × 10⁻⁶. 8 CFU / mL Rhodopseudomonas palustris bacterial suspension; after filtering the Rhodopseudomonas palustris bacterial suspension with filter paper, it was dried at low temperature (30℃) to obtain Rhodopseudomonas palustris bacterial powder. Candida utilis was activated and cultured in liquid culture medium recommended by the China Industrial Microbial Culture Collection Center, followed by fermentation culture, yielding an effective viable count of 2.0 × 10⁻⁶ cells. 8 CFU / mL Candida utilis culture; after filtering the Candida utilis culture with filter paper, dry it at low temperature (30℃) to obtain Candida utilis powder. Bacillus subtilis was activated and cultured in liquid culture medium recommended by the China Industrial Microbial Culture Collection Center, followed by fermentation culture, yielding an effective viable count of 2.0 × 10⁻⁶. 9 Bacillus subtilis bacterial suspension at cfu / mL; after filtering the Bacillus subtilis bacterial suspension with filter paper, it was dried at low temperature (30℃) to obtain Bacillus subtilis bacterial powder; Bacillus megaterium was activated and cultured in liquid culture medium recommended by the China Industrial Microbial Culture Collection Center, followed by fermentation culture, yielding an effective viable count of 1.0 × 10⁻⁶. 9 A CFU / mL Bacillus megaterium culture was prepared; the culture was then filtered through filter paper and dried at a low temperature (30°C) to obtain Bacillus megaterium powder.

[0040] Six parts of Bacillus jelly-like bacterial powder, one part of Lactobacillus acidophilus bacterial powder, 3.5 parts of Rhodopseudomonas palustris bacterial powder, two parts of Candida utilis bacterial powder, six parts of Bacillus subtilis bacterial powder, and four parts of Bacillus megaterium bacterial powder were mixed evenly to obtain a microbial complex.

[0041] (3) Preparation method of sugarcane leaf fermentation product Sugarcane leaves (with a moisture content of about 20%) are crushed to 50 mesh and then mixed with honey and fermentation bacteria (the mass ratio of sugarcane leaves, honey and fermentation bacteria is 100:1.5:0.8). After mixing evenly, anaerobic fermentation is carried out (the temperature of anaerobic fermentation is 38℃ and the time is 54h) to obtain sugarcane leaf fermentation product.

[0042] The fermentation bacteria is *Lactobacillus plantarum* powder; the preparation method of *Lactobacillus plantarum* powder is as follows: Lactobacillus plantarum was activated and cultured in liquid culture medium recommended by the China Industrial Microbial Culture Collection Center, followed by fermentation culture, yielding an effective viable count of 1.0 × 10⁻⁶. 8 The *Lactobacillus plantarum* bacterial solution was prepared by filtering the solution with filter paper and then drying it at a low temperature (30°C) to obtain *Lactobacillus plantarum* powder.

[0043] (4) Preparation method of cultivation microbial agent to promote high sugarcane yield A. Mix the microbial complex, oyster shell powder (300 mesh), dolomite powder (300 mesh), and corn cob powder (300 mesh) evenly, spray with a starch aqueous solution with a concentration of 1.8 wt.%, and granulate at low temperature (35℃) to obtain microbial particles (2 mm in diameter). B. Mix sugarcane leaf fermentation product, potato starch, sugarcane bagasse and beet, dry them, and pulverize them to 300 mesh to obtain mixed powder; C. While adding the mixed powder to the microbial granules, spray a starch aqueous solution with a concentration of 2wt.% to coat the surface of the microbial granules. After drying at low temperature (40℃), compound fertilizer granules are obtained. D. Spray an aqueous solution of carboxymethyl cellulose onto the surface of compound fertilizer granules, and dry it (at a temperature of 40°C, repeatedly spray and dry, each time spraying until the surface of all compound fertilizer granules is moist) to obtain a cultivation microbial agent that promotes high sugarcane yield.

[0044] The aqueous solution of carboxymethyl cellulose is prepared by mixing carboxymethyl cellulose and water in a mass ratio of 3:100.

[0045] Example 2 A cultivation inoculant to promote high sugarcane yield: (1) The cultivation agent for promoting high sugarcane yield is composed of the following raw materials in parts by weight: 3 parts microbial complex, 8 parts sugarcane leaf fermentation, 2 parts oyster shell powder, 2 parts dolomite powder, 11 parts corn cob powder, 6 parts potato starch, 2 parts sugarcane bagasse, 2 parts sugar beet and 3.5 parts carboxymethyl cellulose. The moisture content of corn cob powder is about 8%. The moisture content of sugarcane bagasse is about 15%; Beetroot refers to the fresh stems and leaves of the beet.

[0046] (2) Preparation of microbial complexes Same as Example 1, except that 5 parts of Bacillus jellyii powder, 2 parts of Lactobacillus acidophilus powder, 4 parts of Rhodopseudomonas palustris powder, 3 parts of Candida utilis powder, 5 parts of Bacillus subtilis powder and 5 parts of Bacillus megaterium powder are mixed evenly to obtain a microbial complex.

[0047] (3) Preparation method of sugarcane leaf fermentation product Sugarcane leaves (with a moisture content of about 20%) are crushed to 50 mesh and then mixed with honey and fermentation bacteria (the mass ratio of sugarcane leaves, honey and fermentation bacteria is 100:1:0.5). After mixing evenly, anaerobic fermentation is carried out (the temperature of anaerobic fermentation is 35℃ and the time is 60h) to obtain sugarcane leaf fermentation product.

[0048] The fermentation bacteria is *Lactobacillus plantarum* powder; the preparation method of *Lactobacillus plantarum* powder is as follows: Lactobacillus plantarum was activated and cultured in liquid culture medium recommended by the China Industrial Microbial Culture Collection Center, followed by fermentation culture, yielding an effective viable count of 1.0 × 10⁻⁶. 8 The *Lactobacillus plantarum* bacterial solution was prepared by filtering the solution with filter paper and then drying it at a low temperature (30°C) to obtain *Lactobacillus plantarum* powder.

[0049] (4) Preparation method of cultivation microbial agent to promote high sugarcane yield A. Mix the microbial complex, oyster shell powder (300 mesh), dolomite powder (300 mesh), and corn cob powder (300 mesh) evenly, spray with a starch aqueous solution with a concentration of 1.5 wt.%, and granulate at low temperature (35℃) to obtain microbial particles (1.5 mm in diameter). B. Mix sugarcane leaf fermentation product, potato starch, sugarcane bagasse and beet, dry them, and then pulverize them to 250 mesh to obtain mixed powder; C. While adding the mixed powder to the microbial granules, spray a starch aqueous solution with a concentration of 2wt.% to coat the surface of the microbial granules. After drying at low temperature (40℃), compound fertilizer granules are obtained. D. Spray an aqueous solution of carboxymethyl cellulose onto the surface of compound fertilizer granules, and dry it (at a temperature of 40°C, repeatedly spray and dry, each time spraying until the surface of all compound fertilizer granules is moist) to obtain a cultivation microbial agent that promotes high sugarcane yield.

[0050] The aqueous solution of carboxymethyl cellulose is prepared by mixing carboxymethyl cellulose and water in a mass ratio of 2.5:100.

[0051] Example 3 A cultivation inoculant to promote high sugarcane yield: (1) The cultivation agent for promoting high sugarcane yield is composed of the following raw materials in parts by weight: 4 parts of microbial complex, 5 parts of sugarcane leaf fermentation, 2.5 parts of oyster shell powder, 1 part of dolomite powder, 10 parts of corn cob powder, 5 parts of potato starch, 3 parts of sugarcane bagasse, 3 parts of sugar beet and 2.5 parts of carboxymethyl cellulose. The moisture content of corn cob powder is about 8%. The moisture content of sugarcane bagasse is about 15%; Beetroot refers to the fresh stems and leaves of the beet.

[0052] (2) Preparation of microbial complexes Same as Example 1, except that 8 parts of Bacillus jelly-like powder, 2 parts of Lactobacillus acidophilus powder, 3 parts of Rhodopseudomonas palustris powder, 2.5 parts of Candida utilis powder, 5 parts of Bacillus subtilis powder and 4.5 parts of Bacillus megaterium powder are mixed evenly to obtain a microbial complex.

[0053] (3) Preparation method of sugarcane leaf fermentation product Sugarcane leaves (with a moisture content of about 20%) are crushed to 50 mesh and then mixed with honey and fermentation bacteria (the mass ratio of sugarcane leaves, honey and fermentation bacteria is 1000:2:1). After mixing evenly, anaerobic fermentation is carried out (the temperature of anaerobic fermentation is 40℃ and the time is 48h) to obtain sugarcane leaf fermentation product.

[0054] The fermentation bacteria is *Lactobacillus plantarum* powder; the preparation method of *Lactobacillus plantarum* powder is as follows: Lactobacillus plantarum was activated and cultured in liquid culture medium recommended by the China Industrial Microbial Culture Collection Center, followed by fermentation culture, yielding an effective viable count of 1.0 × 10⁻⁶. 8 The *Lactobacillus plantarum* bacterial solution was prepared by filtering the solution with filter paper and then drying it at a low temperature (30°C) to obtain *Lactobacillus plantarum* powder.

[0055] (4) Preparation method of cultivation microbial agent to promote high sugarcane yield A. Mix the microbial complex, oyster shell powder (300 mesh), dolomite powder (300 mesh), and corn cob powder (300 mesh) evenly, spray with a starch aqueous solution with a concentration of 2wt.%, and granulate at low temperature (35℃) to obtain microbial particles (2 mm in diameter). B. Mix sugarcane leaf fermentation product, potato starch, sugarcane bagasse and beet, dry them, and grind them to 200 mesh to obtain mixed powder; C. While adding the mixed powder to the microbial granules, spray a starch aqueous solution with a concentration of 2wt.% to coat the surface of the microbial granules. After drying at low temperature (40℃), compound fertilizer granules are obtained. D. Spray an aqueous solution of carboxymethyl cellulose onto the surface of compound fertilizer granules, and dry it (at a temperature of 40°C, repeatedly spray and dry, each time spraying until the surface of all compound fertilizer granules is moist) to obtain a cultivation microbial agent that promotes high sugarcane yield.

[0056] The aqueous solution of carboxymethyl cellulose is prepared by mixing carboxymethyl cellulose and water in a mass ratio of 3.5:100.

[0057] Comparative Example 1 Same as Example 1, except that the cultivation agent for promoting high sugarcane yield does not contain corn cob powder.

[0058] Comparative Example 2 Same as Example 1, except that the corn cob powder in the cultivation agent that promotes high sugarcane yield is replaced with an equal mass of rice straw powder. The moisture content of rice straw powder is about 8%.

[0059] Comparative Example 3 Same as Example 1, except that the microbial complex does not contain Bacillus jellyoides powder and Candida utilis powder.

[0060] Comparative Example 4 Same as Example 1, except that the gelatinous Bacillus powder in the microbial complex is replaced with an equal mass of Lactobacillus plantarum powder; The preparation method of Lactobacillus plantarum powder is as follows: Lactobacillus plantarum was activated and cultured in liquid culture medium recommended by the China Industrial Microbial Culture Collection Center, followed by fermentation culture, yielding an effective viable count of 1.0 × 10⁻⁶. 8 The *Lactobacillus plantarum* bacterial solution was prepared by filtering the solution with filter paper and then drying it at a low temperature (30°C) to obtain *Lactobacillus plantarum* powder.

[0061] Comparative Example 5 Same as Example 1, except that sugar beets are replaced with an equal amount of sugarcane bagasse.

[0062] Comparative Example 6 Same as Example 1, except that the preparation method of the cultivation inoculant for promoting high sugarcane yield is as follows: A. Mix the microbial complex, oyster shell powder (300 mesh), dolomite powder (300 mesh), and corn cob powder (300 mesh) evenly to obtain a microbial mixture; B. Mix sugarcane leaf fermentation product, potato starch, sugarcane bagasse and beet, dry them, and pulverize them to 300 mesh to obtain mixed powder; C. Mix the microbial mixture and the mixed powder evenly, spray with a starch aqueous solution with a concentration of 1.8 wt.%, and granulate at low temperature (35℃) to obtain microbial particles (particle size of 2 mm). D. A cultivation agent that promotes high sugarcane yield is obtained by spraying an aqueous solution of carboxymethyl cellulose onto the surface of microbial particles, drying it (at a temperature of 40°C, repeatedly spraying and drying, each time spraying until the surface of all compound fertilizer particles is moist).

[0063] Example 1 (1) The experiment was conducted in Ziyuan County, Guangxi, a region prone to frost. The randomized block design was used with three replicates. Each plot had five rows, with a row length of 7m and a row spacing of 1.2m. On March 13, 2014, the soil was tilled and the cultivation inoculants prepared in the examples or comparative examples were applied to three plots. The application rate was 5kg / mu. After mixing with the soil and letting stand for 7 days, sugarcane (the variety was Xintai Sugar 22 (ROC22)) was planted. The seeding rate was 16 buds per meter. After planting and covering with soil, herbicides were sprayed and mulch was applied. Other management was carried out in accordance with conventional field production.

[0064] (2) In early September, the same cultivation agent was applied again to each plot. The application method was trench application, and the application amount was 5 kg / mu. Other management was carried out in accordance with conventional field production. Water was applied in time during the dry autumn and winter seasons to avoid abnormal leaf death due to drought.

[0065] A plot without the application of cultivation inoculant was used as a control group.

[0066] Table 1 Meteorological Information A survey on the green leaf rate was conducted on December 31, 2024, and the results are shown in Table 2.

[0067] Green leaf rate (%) = (total green leaf area / total leaf area) × 100%.

[0068] Table 2 Green leaf rate of sugarcane After the sugarcane is harvested, its sugar content is measured, and the yield is tallied and calculated.

[0069] Different sugarcane were randomly selected, and the middle section of the internode was cut off. After removing the outer skin, the sucrose content was determined according to GB / T 10499-2014. The results are shown in Table 3.

[0070] Table 3 Sugarcane Quality The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A cultivation microbial agent for promoting high sugarcane yield, characterized in that, The raw materials include the following parts by weight: 3-4 parts of microbial complex, 5-8 parts of sugarcane leaf ferment, 2-3 parts of oyster shell, 1-2 parts of dolomite powder, 10-12 parts of corn cob, 5-6 parts of potato starch, 2-3 parts of sugarcane bagasse, 2-3 parts of sugar beet, and 2.5-3.5 parts of carboxymethyl cellulose. The microbial complex comprises the following components in parts by weight: 5-8 parts of Bacillus jellyii, 1-2 parts of Lactobacillus acidophilus, 3-4 parts of Rhodopseudomonas palustris, 2-3 parts of Candida utilis, 5-6 parts of Bacillus subtilis, and 4-5 parts of Bacillus megaterium.

2. The cultivation inoculant according to claim 1, characterized in that, The method for preparing the sugarcane leaf ferment includes the following steps: Sugarcane leaves are crushed, then honey and fermentation bacteria are added, mixed evenly, and then subjected to anaerobic fermentation to obtain the sugarcane leaf fermentation product.

3. The cultivation inoculant according to claim 2, characterized in that, The mass ratio of sugarcane leaves, honey, and fermentation bacteria is 100:(1~2):(0.5~1).

4. The cultivation inoculant according to claim 2, characterized in that, The fermentation bacteria include Lactobacillus plantarum; And / or, the anaerobic fermentation temperature is 35~40℃ and the time is 48~60h.

5. A method for preparing the cultivation inoculant according to any one of claims 1 to 4, characterized in that, Includes the following steps: (1) Mix the microbial complex, oyster shell, dolomite powder and corn cob evenly, spray with starch aqueous solution, and granulate at low temperature to obtain microbial particles; (2) Mix sugarcane leaf fermentation, potato starch, sugarcane bagasse and beet, dry them, and then pulverize them to obtain mixed powder; (3) The mixed powder is coated on the surface of the microbial particles to obtain compound fertilizer particles; (4) Spray an aqueous solution of carboxymethyl cellulose onto the surface of the compound fertilizer granules, and dry them to obtain the cultivation agent that promotes high sugarcane yield.

6. The preparation method according to claim 5, characterized in that, In step (1), the concentration of the starch aqueous solution is 1.5~2 wt.%; And / or, in step (1), the particle size of the microbial particles is 1.5~2mm.

7. The preparation method according to claim 5, characterized in that, In step (2), the particle size of the mixed powder is 200-300 mesh; And / or, in step (4), the aqueous solution of carboxymethyl cellulose is prepared by mixing carboxymethyl cellulose and water in a mass ratio of (2.5~3.5):

100.

8. The preparation method according to claim 5, characterized in that, In step (1), the temperature for low-temperature granulation is 35~45℃; And / or, in step (4), the drying temperature is 40~45℃.

9. The application of the cultivation inoculant according to any one of claims 1 to 4 in sugarcane cultivation.

10. The use of the cultivation agent according to any one of claims 1 to 4 in the preparation of an antifreeze composition for improving the cold resistance of sugarcane.

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