An integrated cell culture and sampling device

Abstract

The application discloses an integrated cell culture and sampling device and belongs to the technical field of cell culture. In the application, the crank rotating shaft, the cover plate, the moving slide, the electric telescopic rod and the negative pressure suction disc are arranged, the negative pressure suction disc and the adsorbed fixed cover plate accurately return to the position directly above the culture dish, then the control system controls the electric telescopic rod to go down and fine tune, the cover plate is accurately attached and closed on the top of the culture dish, finally, the negative pressure suction function of the negative pressure suction disc is closed, the cover plate is loosened by the negative pressure suction disc, the cover plate completely seals the culture dish, and the whole process operation of single culture dish opening, sampling and closing is completed. After the single-group sampling operation is completed, the equipment controls the cross-shaped turntable to switch the fixed angle position, transfers the new culture dish to be sampled to the operation position, then the whole set of procedures of the above-mentioned driving transmission, mechanism translation, opening, sampling, resetting and closing are repeated, and the circulating and continuous sampling operation of multiple culture dishes is carried out, so that the automatic and batch sampling processing of the equipment is realized.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, and in particular relates to an integrated cell culture and sampling device. Background Technology

[0002] Cell culture technology is a crucial fundamental experimental technique in modern biology, biomedicine, and pharmacology and toxicology research. It is widely used in numerous experimental scenarios, including exploring cell biological mechanisms, screening in vitro drug efficacy, evaluating toxicity, stem cell research, and developing biopharmaceuticals. In various in vitro cell-related studies, target cells are typically seeded into culture dishes containing appropriate culture media. These dishes are then placed under suitable temperature, humidity, and gaseous conditions for constant-temperature incubation, providing a stable in vitro microenvironment for cell growth, proliferation, and metabolic activities. Throughout the incubation period, researchers need to periodically sample and analyze the cells and supernatant within the culture dishes according to pre-set experimental timeframes. By observing, measuring biochemical indicators, and performing molecular biological tests on the obtained cell samples, researchers can dynamically track and monitor cell proliferation rates, metabolic levels, and survival / apoptosis status. Simultaneously, they can observe the effects of drugs or external stimuli on cell growth behavior, providing reliable sample data support for subsequent experimental data analysis and conclusion derivation.

[0003] Existing integrated cell culture and sampling devices require repeated manual opening and removal of the lid when sampling from culture dishes, which easily leads to bacterial contamination of cell samples. Reusing pipette tips can also cause cross-contamination between different groups of samples, directly affecting the reliability of subsequent test results. When conducting batch experiments with multiple groups of cell samples, the entire process of manually opening the lid, changing pipette tips, sampling, and storing samples is cumbersome, labor-intensive, and inefficient. Individual differences in the operating techniques of different experimenters can also lead to poor consistency in experimental operations and difficulty in ensuring experimental reproducibility. Therefore, there is an urgent need for an integrated cell culture and sampling device to solve the above problems. Summary of the Invention

[0004] The purpose of this invention is to address the problems of existing integrated cell culture and sampling devices, which require repeated manual opening and removal of the lid when sampling from culture dishes. This easily leads to bacterial contamination of cell samples, and the reuse of pipette tips can cause cross-contamination between different groups of samples, directly affecting the reliability of subsequent test results. When conducting batch experiments with multiple groups of cell samples, the entire process of manually opening the lid, changing pipette tips, sampling, and storing samples is cumbersome, labor-intensive, and inefficient. Individual differences in the operating techniques of different experimental personnel can also lead to poor consistency in experimental operations and difficulty in ensuring experimental reproducibility. Therefore, this invention proposes an integrated cell culture and sampling device.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] An integrated cell culture and sampling device includes: a mounting base, a sealing shell fixedly mounted on the top edge of the mounting base, the sealing shell surrounding the upper edge of the mounting base to form a closed cell culture operation cavity, a sampling robotic arm fixedly mounted on one side of the top of the mounting base and located inside the cavity enclosed by the sealing shell, a driving mechanism fixedly mounted on the inner side of the mounting base, a station switching mechanism rotatably mounted on the top of the mounting base via a bearing, and a moving adsorption mechanism located on the top of the mounting base inside the sealing shell, between the sampling robotic arm and the station switching mechanism.

[0007] As a further description of the above technical solution:

[0008] The mounting base has a mounting groove 1 at its bottom, and a mounting groove 2 at the top of the mounting groove 1 on its inner side. Rectangular sliding grooves are provided through the top and bottom of the two sides of the mounting groove 2. Rectangular placement grooves are symmetrically provided through the edge of one side of the mounting base. Sample boxes or sampling gun tip boxes are respectively snapped into the rectangular placement grooves. An annular guide groove is provided at the top of the mounting base at the position corresponding to the workstation switching mechanism.

[0009] As a further description of the above technical solution:

[0010] The driving mechanism includes a drive motor, which is fixedly connected to the bottom of the first mounting slot. A drive bevel gear is fixedly connected to the output end of the drive motor. A driven bevel gear is rotatably mounted on the bottom of the first mounting slot, located on one side of the output end of the drive motor, via a bearing. The driven bevel gear meshes with the drive bevel gear. A push crank shaft is fixedly mounted on the top of the driven bevel gear. The top of the push crank shaft is rotatably connected to the top of the first mounting slot via a bearing. The top of the push crank shaft extends through the first mounting slot and into the second mounting slot, where a mounting turntable is fixedly connected. A dial is fixedly mounted on the top of the mounting turntable, and a cylindrical pin is fixedly mounted on the edge of the top of the mounting turntable on one side of the dial.

[0011] As a further description of the above technical solution:

[0012] The workstation switching mechanism includes an intermittent turntable, and the top and bottom of the intermittent turntable are rotatably connected to the inner side of the second mounting groove via bearings. The outer circular surface of the intermittent turntable is provided with four arc-shaped sliding grooves at equal angles, and the arc-shaped sliding grooves are in close contact with the outer circular surface of the dial. The outer circular surface of the intermittent turntable is provided with an intermittent locking groove between two adjacent arc-shaped sliding grooves, and the intermittent locking grooves are correspondingly engaged with cylindrical pins. The top of the intermittent turntable extends through the second mounting groove to the top of the mounting base and is fixedly connected to a cross turntable, and the cross turntable is in close contact with the top of the mounting base.

[0013] As a further description of the above technical solution:

[0014] The four corners of the cross turntable are respectively provided with placement slots, and the placement slots correspond to the positions of the arc-shaped sliding grooves. A petri dish is placed inside the placement slot, and the top of the petri dish is covered with a cover plate. The four corners of the cross turntable are respectively provided with rectangular mounting grooves on both sides of the placement slots, and limit sliding columns are symmetrically fixedly installed on the inner walls of the rectangular mounting grooves. A fixing protrusion is fixedly connected to one side of the cross turntable located on one side of the rectangular mounting groove, and an L-shaped rotating strip is rotatably connected through the surface of the fixing protrusion.

[0015] As a further description of the above technical solution:

[0016] The four corners of the cross turntable are provided with concave top plates at the bottom of the placement slots, and the two sides of the concave top plates are slidably engaged with the inside of the rectangular mounting slots. The two sides of the concave top plates are respectively provided with symmetrical limiting slide grooves, and the limiting slide grooves are slidably connected to the surface of the limiting slide column. The center positions of the two sides of the concave top plates are respectively fixedly connected with push top blocks, and one end of the L-shaped rotating bar is slidably connected to the surface of the push top block through the slide groove.

[0017] As a further description of the above technical solution:

[0018] The bottom of the cross turntable is provided with a movable slide groove near the placement slot, and the inner wall of the movable slide groove is symmetrically fixedly connected with a mounting slide column. The inner side of the movable slide groove is slidably connected with a movable slide bar, and the movable slide bar is slidably connected to the surface of the mounting slide column. The two ends of the movable slide bar are fixedly connected with push protrusions, and the push protrusions are slidably connected to the inside of the slide groove at the bottom of the L-shaped rotating bar. The center of the bottom of the movable slide bar is fixedly connected with a guide slide column, and the guide slide column is slidably connected to the inside of the annular guide slide groove.

[0019] As a further description of the above technical solution:

[0020] The movable adsorption mechanism includes two limiting slides, which are fixedly connected to the inner wall of the mounting groove. The height of the limiting slides corresponds to the height of the crank of the push crank shaft. A movable slide is slidably connected through the surface between the two limiting slides. A push groove is provided through the center of the movable slide, and the crank of the push crank shaft is slidably connected through the inside of the push groove. Rectangular connecting plates are fixedly connected to the tops of both ends of the movable slide, and the rectangular connecting plates are slidably connected through the inside of the rectangular groove. The top of the rectangular connecting plates extends through the rectangular groove to the top of the mounting base. A mounting plate is fixedly connected between the tops of the two rectangular connecting plates on opposite sides. An electric telescopic rod is embedded and fixedly installed at the center of the top of the mounting plate, and the telescopic end of the electric telescopic rod extends through the mounting plate to the bottom of the mounting plate and is fixedly connected to a negative pressure suction cup.

[0021] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are: 1. In this invention, by setting up a crank shaft, cover plate, movable carriage, electric telescopic rod, and negative pressure suction cup, when the equipment starts sampling, the drive motor is controlled to drive the drive bevel gear to rotate. Through the bevel gear meshing transmission structure, the drive bevel gear precisely drives the driven bevel gear to rotate, thereby controlling the push crank shaft to perform a stable circular rotation. During the rotation of the push crank shaft, its end crank structure is nested inside the push slide groove and slides, converting the circular rotation of the shaft into linear reciprocating power, continuously pushing the movable carriage to perform horizontal reciprocating sliding between two sets of symmetrically arranged limiting carriages. While the movable carriage is reciprocating, it drives the fixedly connected rectangular connecting plate to move synchronously, and the rectangular connecting plate is linked to pull... The mounting plate, electric telescopic rod, and negative pressure suction cup move back and forth on the top of the mounting base, enabling the negative pressure suction cup to switch positions. When the negative pressure suction cup moves with the mechanism, moves away from the sampling robotic arm, and precisely moves to the position directly above the cover plate, the equipment control system triggers the electric telescopic rod to quickly extend and descend, pushing the negative pressure suction cup at the bottom to tightly adhere to the upper surface of the cover plate. The negative pressure suction cup activates its negative pressure adsorption function, firmly adsorbing and fixing the cover plate. Subsequently, the drive mechanism continues to transmit power, driving the moving adsorption mechanism to move in the opposite direction, smoothly removing the cover plate from the top of the culture dish, completely opening the culture dish and exposing the internal culture medium and experimental cells, providing working space for sampling. Simultaneously with the removal of the cover plate, the equipment control system... The sampling robotic arm starts working, automatically grabbing a brand-new sampling tip from the sampling tip box pre-set on the mounting base, completing the tip replacement operation. This eliminates cross-contamination caused by reusing tips and ensures the purity and detection accuracy of the experimental samples. After the tip replacement is completed, the sampling robotic arm, carrying the new tip, precisely inserts it into the open culture dish to complete the cell sampling and sample collection. After the sampling process is completed, the sampling robotic arm returns to its original position, placing the collected experimental sample along with the sampling tip into the sample box on the mounting base for sample storage. This facilitates subsequent unified testing and processing. While the sample is being stored, the drive mechanism continuously rotates the push crank shaft, pushing the moving carriage to slide in the reverse direction. The system resets the negative pressure suction cup and the fixed cover plate back to the position directly above the culture dish. Then, the control system controls the electric telescopic rod to make a slight adjustment so that the cover plate fits precisely on the top of the culture dish. Finally, the negative pressure suction cup is turned off, the negative pressure suction cup releases the cover plate, and the cover plate completely seals the culture dish, completing the entire process of opening, sampling, and closing the culture dish for a single operation. After a single sampling operation is completed, the equipment controls the cross turntable to switch to a fixed angle position, transferring the new culture dish to be sampled to the work position. Then, the entire process of drive transmission, mechanism translation, opening and sampling, and resetting and closing is repeated to continuously sample multiple groups of culture dishes, realizing automated batch sampling and processing. 2. In this invention, by setting up a crankshaft, mounting turntable, intermittent turntable, cross turntable, L-shaped rotating bar, concave top plate, and movable slide bar, the drive motor of the starting drive mechanism serves as the core power source during normal operation. The output torque of the drive motor drives the drive bevel gear to rotate at high speed. Through the bevel gear meshing transmission principle, the drive bevel gear drives the driven bevel gear to rotate synchronously, completing the direction and transmission of power. Under the drive of gear transmission, the crankshaft is pushed to maintain synchronous and uniform rotation with the mounting turntable. The dial on the outer side of the mounting turntable always conforms to the arc-shaped slide groove on the surface of the intermittent turntable to perform circumferential follow-up motion. The curved sliding groove ensures precise and stable rotation of the dial, preventing any deviation or wobbling. When the equipment reaches a designated workstation angle, the cylindrical pin mounted on the dial precisely engages with the intermittent slot of the intermittent turntable, driving the intermittent turntable to rotate the cross turntable at a fixed angle intermittently. This achieves precise switching between workstations. In the standard workstation switching process, the cross turntable intermittently transports culture dishes containing culture media and experimental cells to designated workstations. When the cells inside the culture dish complete the predetermined culture process and require sampling or removal, the drive motor... The system operates continuously and stably, providing power to the entire mechanism and driving the intermittent turntable and cross turntable to maintain intermittent rotation, continuously completing the workstation rotation operation. During the rotation of the cross turntable, the movable slide bar mounted at its bottom slides against the equipment's reference working surface, while the guide column at the end of the movable slide bar slides inside the corresponding annular guide groove of the equipment. As the workstation continues to switch, the movable slide bar gradually moves away from the sampling robot arm, and its bottom guide column slides along the annular guide groove to the raised workstation of the groove body. Due to the limiting and pushing action of the raised structure, the guide column pushes the movable slide bar to slide in a straight line along the surface of the mounting column. During the sliding motion of the moving slider, the pushing protrusion is driven to conform to the L-shaped rotating bar, causing the L-shaped rotating bar to rotate around the fixed protrusion. Based on the lever rotation principle of the L-shaped rotating bar, its other end is lifted in the opposite direction, precisely pushing the top block upward, and finally driving the concave top plate to move upward synchronously. Through the vertical pushing force of the concave top plate, the culture dish that has been cultured inside the slot can be stably pushed out as a whole, so that the culture dish is freed from the slot limit. The operator can conveniently and quickly complete the culture dish removal operation without manually picking or prying it, avoiding bumping the culture dish during the removal process and causing damage to the culture dish. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the unfolded structure of an integrated cell culture and sampling device proposed in this invention; Figure 2 This is a cross-sectional view of the integrated cell culture and sampling device proposed in this invention. Figure 3 This is a cross-sectional view of the mounting base and sealing shell of an integrated cell culture and sampling device proposed in this invention. Figure 4 This is a schematic diagram of the mounting base and annular guide groove of the integrated cell culture and sampling device proposed in this invention; Figure 5 This is a schematic diagram of the drive mechanism, station switching mechanism, and moving adsorption mechanism of an integrated cell culture and sampling device proposed in this invention. Figure 6 This is a schematic diagram of the drive motor and push crank shaft of the integrated cell culture and sampling device proposed in this invention; Figure 7 This is a schematic diagram of the mounting turntable and intermittent turntable of an integrated cell culture and sampling device proposed in this invention; Figure 8 This is a schematic diagram of the intermittent turntable and cross turntable of an integrated cell culture and sampling device proposed in this invention; Figure 9 This is a schematic diagram of the cross-shaped rotating disk and concave top plate of the integrated cell culture and sampling device proposed in this invention. Figure 10 This is a schematic diagram of the cross-shaped turntable and placement slot of an integrated cell culture and sampling device proposed in this invention; Figure 11 This is a schematic diagram of the concave top plate and the movable slider of an integrated cell culture and sampling device proposed in this invention. Figure 12 This is a schematic diagram of the moving slide and mounting plate of an integrated cell culture and sampling device proposed in this invention.

[0023] Legend: 1. Mounting base; 101. Mounting slot one; 102. Mounting slot two; 103. Rectangular slide; 104. Rectangular placement slot; 105. Sample box; 106. Sampling nozzle box; 107. Annular guide slide; 2. Sealing cover; 3. Sampling robotic arm; 4. Drive mechanism; 401. Drive motor; 402. Drive bevel gear; 403. Driven bevel gear; 404. Push crank shaft; 405. Mounting turntable; 406. Dial; 407. Cylindrical pin; 5. Station switching mechanism; 501. Intermittent turntable; 502. Arc-shaped slide; 503. Intermittent slot; 504. Cross turntable; 5 5. Placement slot; 506. Petri dish; 507. Cover plate; 508. Rectangular mounting groove; 509. Limiting slide column; 510. Fixing protrusion; 511. L-shaped rotating bar; 512. Concave top plate; 513. Limiting slide groove; 514. Pushing top block; 515. Moving slide groove; 516. Mounting slide column; 517. Moving slide bar; 518. Pushing protrusion; 519. Guide slide column; 6. Moving adsorption mechanism; 601. Limiting slide frame; 602. Moving slide frame; 603. Pushing slide groove; 604. Rectangular connecting plate; 605. Mounting connecting plate; 606. Electric telescopic rod; 607. Negative pressure suction cup. Detailed Implementation

[0024] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0025] In its specific implementation, such as Figures 1-12 The present invention provides a technical solution:

[0026] An integrated cell culture and sampling device includes: a mounting base 1; a sealing shell 2 fixedly mounted on the top edge of the mounting base 1, the sealing shell 2 enclosing the upper edge of the mounting base 1 to form a closed cell culture operation cavity; a sampling robotic arm 3 fixedly mounted on one side of the top of the mounting base 1, the sampling robotic arm 3 being located inside the cavity enclosed by the sealing shell 2; and rectangular placement slots 104 symmetrically extending through one side edge of the mounting base 1, each containing a sample box 105 or a sampling pipette tip box 106. The sealing shell 2 encloses the sampling and cell culture operation area. The closed and isolated design can prevent external dust and bacteria from entering the inner cavity, avoiding environmental contamination of cell samples. The mounting base 1 provides the mounting bearing benchmark for all mechanisms. The sampling robotic arm 3, drive mechanism 4, station switching mechanism 5, and moving adsorption mechanism 6 are centrally arranged on the same equipment body, realizing the integrated operation of cell culture, station rotation, opening and closing of cover plate 507, and sampling. It eliminates the need for multiple devices to transport culture dishes 506, reducing the experimental operation process. The rectangular placement slot 104 uses a snap-fit ​​installation method for sample box 105 and sampling pipette tip box 106, making it convenient for experimental personnel to quickly pull out and replace consumable boxes.

[0027] A drive mechanism 4 is fixedly installed inside the mounting base 1. The bottom of the mounting base 1 has a mounting groove 101, and the top of the mounting groove 101 has a mounting groove 102. The drive mechanism 4 includes a drive motor 401, which is fixedly connected to the bottom of the mounting groove 101. A drive bevel gear 402 is fixedly connected to the output end of the drive motor 401. A driven bevel gear 403 is rotatably mounted on one side of the bottom of the mounting groove 101, located at the output end of the drive motor 401, via a bearing. The driven bevel gear 403 meshes with the drive bevel gear 402. A push crank shaft 404 is fixedly mounted on the top of the driven bevel gear 403, and the top end of the push crank shaft 404 is rotatably connected to the top of the mounting groove 101 via a bearing. The top end of the push crank shaft 404 extends through the mounting groove 101. An installation turntable 405 is fixedly connected inside the installation slot 2 102. A dial 406 is fixedly installed on the top of the installation turntable 405, and a cylindrical pin 407 is fixedly installed on the edge of the top of the installation turntable 405 on one side of the dial 406. When the equipment is working, the drive motor 401 outputs rotational power, which is driven by the bevel gear 402 and the driven bevel gear 403 to realize power reversal and torque transmission. This synchronously drives the push crank shaft 404 and the installation turntable 405 to rotate continuously. A single drive motor 401 provides power to both the station switching mechanism 5 and the moving adsorption mechanism 6, reducing the number of power components and lowering equipment costs and control difficulty. The dial 406 and the cylindrical pin 407 follow the installation turntable 405 to perform circular motion synchronously, providing power input for the intermittent indexing action of the station switching mechanism 5.

[0028] The inner side of the mounting base 1 is provided with a second mounting groove 102 at the top of the first mounting groove 101. A workstation switching mechanism 5 is rotatably mounted on the top of the mounting base 1 via a bearing. The workstation switching mechanism 5 includes an intermittent turntable 501, and the top and bottom of the intermittent turntable 501 are rotatably connected to the inner side of the second mounting groove 102 via bearings. The outer circular surface of the intermittent turntable 501 has four arc-shaped sliding grooves 502 at equal angles, and the arc-shaped sliding grooves 502 are in close contact with the outer circular surface of the dial 406. An intermittent locking groove 503 is provided on the outer circular surface of the intermittent turntable 501 between two adjacent arc-shaped sliding grooves 502, and the intermittent locking groove 503 is correspondingly engaged with the cylindrical pin 407. The top of the intermittent turntable 501 extends through the second mounting groove 102 to the top of the mounting base 1. A cross turntable 504 is fixedly connected to the base, and the cross turntable 504 is close to the top of the mounting base 1. When the dial 406 slides against the arc-shaped slide groove 502, the intermittent turntable 501 remains stationary and the equipment is in the station holding stage. When the cylindrical pin 407 rotates into and gets into the intermittent slot 503, it drives the intermittent turntable 501 and the cross turntable 504 to complete the intermittent rotation at a fixed angle. After the cylindrical pin 407 disengages from the intermittent slot 503, the cross turntable 504 stops rotating again. This mechanism realizes the intermittent indexing of the culture dish 506 station, ensuring that the cross turntable 504 remains stationary during sampling, opening, and ejection operations. The positioning accuracy is high, preventing the culture dish 506 from shaking or shifting during the operation.

[0029] The four corners of the cross turntable 504 are respectively provided with placement slots 505, and the placement slots 505 correspond to the positions of the arc-shaped slides 502. The placement slots 505 contain petri dishes 506, and the top of the petri dishes 506 is covered with a cover plate 507. The four corners of the cross turntable 504 are respectively provided with rectangular mounting slots 508 on both sides of the placement slots 505, and the inner walls of the rectangular mounting slots 508 are symmetrically fixedly installed with limiting slides 509. The side of the cross turntable 504 is fixedly connected to one side of the rectangular mounting slots 508, and the surface of the fixed protrusions 510 is rotatably connected with an L-shaped rotating strip 511. The four corners of the turntable 504 are provided with concave top plates 512 at the bottom of the placement slots 505. The two sides of the concave top plates 512 are slidably engaged with the inside of the rectangular mounting slots 508. Symmetrical limiting grooves 513 are provided on both sides of the concave top plates 512, and the limiting grooves 513 are slidably connected to the surface of the limiting sliding posts 509. Pushing blocks 514 are fixedly connected to the center of both sides of the concave top plates 512, and one end of the L-shaped rotating bar 511 is slidably connected to the surface of the pushing block 514 through a groove. The placement slots 505 provide circumferential limiting for the culture dish 506, preventing the culture dish 506 from slipping and tipping over during the rotation of the turntable 504. The rectangular mounting slots 508 provide vertical movement space for the concave top plates 512, and the fixing protrusions 510 serve as the L-shaped rotating bar 511. The L-shaped rotating bar 511 can swing around the pivot point, converting the horizontal thrust into a vertical upward pushing force, thus achieving a change in the direction of the force. The limiting slide bar 509 and the limiting slide groove 513 cooperate with each other to constrain the concave top plate 512 to move only vertically, eliminating lateral offset. When the L-shaped rotating bar 511 swings, it will lift and push the top block 514 upward, thereby driving the concave top plate 512 to move upward. The upward movement of the concave top plate 512 can lift the culture dish 506 in the placement slot 505 upward, detaching it from the placement slot 505 limit, making it convenient for the operator to remove the culture dish 506. When the L-shaped rotating bar 511 returns to its original position, the concave top plate 512 falls back down, and the culture dish 506 can be stably placed inside the placement slot 505 for cell culture.

[0030] The mounting base 1 is provided with an annular guide groove 107 at the position corresponding to the workstation switching mechanism 5. The main body of the annular guide groove 107 is a smooth annular groove. The bottom height of most sections of the groove is consistent, forming a normal sliding section. At a specific angle position corresponding to the ejection operation of the petri dish 506, the annular guide groove 107 protrudes outward to form a raised workstation section. The smooth annular groove and the raised workstation end of the annular guide groove 107 are connected by an inclined groove.

[0031] The bottom of the cross-shaped turntable 504, near the placement slot 505, is provided with a sliding groove 515. A mounting column 516 is symmetrically fixedly connected to the inner wall of the sliding groove 515. A sliding strip 517 is slidably connected to the inner side of the sliding groove 515 and is slidably connected to the surface of the mounting column 516. Pushing protrusions 518 are fixedly connected to both ends of the sliding strip 517 and are slidably connected to the groove at the bottom of the L-shaped rotating bar 511. A [missing information - likely a device or component] is fixedly connected to the center of the bottom of the sliding strip 517. The guide slide post 519 is slidably connected inside the annular guide groove 107. During the rotation of the cross turntable 504, the guide slide post 519 slides along the trajectory of the annular guide groove 107. When the guide slide post 519 slides to the protruding position of the annular guide groove 107, the guide slide post 519 is pushed, which pushes the movable slide bar 517 to slide horizontally along the mounting slide post 516. The movable slide bar 517 drives the push protrusion 518 to push the bottom end of the L-shaped rotating bar 511 horizontally, driving the L-shaped rotating bar 511 to complete the lever swing.

[0032] Rectangular sliding grooves 103 are provided through the top and bottom of both sides of the mounting groove 2 102. The mounting base 1 is located inside the sealing shell 2 and is located on the top between the sampling robotic arm 3 and the workstation switching mechanism 5. A movable adsorption mechanism 6 is provided. The movable adsorption mechanism 6 includes two limiting slides 601. The two limiting slides 601 are fixedly connected to the inner wall of the mounting groove 101, and the height of the limiting slides 601 corresponds to the height of the crank of the push crank shaft 404. A movable slide 602 is slidably connected through the surface between the two limiting slides 601. A push groove 603 is provided through the center of the movable slide 602, and the crank of the push crank shaft 404 is slidably connected through the inside of the push groove 603. Rectangular connecting plates 604 are fixedly connected to the tops of both ends of the movable slide 602, and the rectangular connecting plates 604 are slidably connected inside the rectangular groove 103. The top of the rectangular connecting plates 604 extends through the rectangular groove 103 to the top of the mounting base 1. A mounting connecting plate 605 is fixedly connected between the tops of the two rectangular connecting plates 604 on opposite sides. An electric telescopic rod 606 is embedded and fixedly installed at the center of the top of the mounting connecting plate 605. The telescopic end of the telescopic rod 606 extends through the mounting plate 605 and is fixedly connected to the bottom of the mounting plate 605 with a negative pressure suction cup 607. When the push crank shaft 404 rotates, the push crank shaft 404 slides inside the push slide groove 603, converting the circular rotation of the push crank shaft 404 into the horizontal reciprocating linear motion of the moving slide 602. The limiting slide 601 and the rectangular slide groove 103 together provide bidirectional limiting for the moving slide 602 and the rectangular connecting plate 604. To ensure smooth and unobstructed reciprocating motion of the mobile adsorption mechanism 6, the reciprocating movement of the mobile slide 602 drives the overall horizontal displacement of the mounting plate 605, the electric telescopic rod 606, and the negative pressure suction cup 607. The electric telescopic rod 606 realizes the vertical lifting and lowering of the negative pressure suction cup 607. The negative pressure suction cup 607 uses negative pressure adsorption to grasp and release the cover plate 507, completing the automatic opening and closing of the culture dish 506, replacing manual handling of the cover plate 507, and reducing sample contamination caused during the opening process.

[0033] Working principle:

[0034] A culture dish 506 containing culture medium and cells to be tested is placed inside the placement slots 505 at the four corners of the cross-shaped turntable 504. The cover plate 507 is then placed on top, sealing the shell 2 to create a closed, sterile inner cavity, isolating external bacteria and providing a clean environment for cell culture. After the equipment is started, the drive motor 401 outputs rotational torque, which, through the meshing of the drive bevel gear 402, drives the driven bevel gear 403 to complete the power reversal. The driven bevel gear 403 synchronously drives the push crank shaft 404 and the mounting turntable 405 to rotate continuously. The mounting turntable 405 drives the dial 406 and the cylindrical pin 407 to perform circumferential rotation. The dial 406 rolls along the arc-shaped groove 502 on the outer circumference of the intermittent turntable 501. During this stage, the cylindrical pin 407 is not in the intermittent slot 503, the intermittent turntable 501 remains locked and stationary, the cross turntable 504 is fixed in position, and each culture dish 506 remains in its current position, stably carrying out cell incubation and culture. When the cylindrical pin 407 rotates into the intermittent slot 503, it engages and drives the intermittent turntable 501 to rotate through a set angle, driving the cross turntable 504 to complete one indexing rotation, transferring the next set of culture dishes 506 to the sampling work position. After the cylindrical pin 407 rotates out of the intermittent slot 503, the intermittent turntable 501 locks again, and the cross turntable 504 stops rotating, ensuring that the position of the culture dish 506 is stable during the opening and sampling operations, and that there will be no shaking or displacement.

[0035] Meanwhile, the crank end of the push crankshaft 404 slides within the push groove 603 of the movable slide 602, converting the circular rotation of the push crankshaft 404 into the horizontal reciprocating linear motion of the movable slide 602. The movable slide 602 is constrained and guided by the limiting slide 601, allowing only horizontal sliding. The movable slide 602 slides along the rectangular groove 103 via the rectangular connecting plate 604, driving the mounting connecting plate 605, the electric telescopic rod 606, and the negative pressure suction cup 607 to move together in the mounting... The base 1 moves back and forth horizontally. When the negative pressure suction cup 607 moves horizontally to the position directly above the target culture dish 506, the electric telescopic rod 606 extends downward, driving the negative pressure suction cup 607 to press tightly against the upper surface of the cover plate 507. The negative pressure suction cup 607 activates negative pressure and firmly adheres to the cover plate 507. Then the electric telescopic rod 606 retracts, driving the negative pressure suction cup 607 to move upward, removing the cover plate 507 from above the culture dish 506 as a whole, and the inner cavity of the culture dish 506 is fully exposed, ready for sampling operation.

[0036] At the same moment the cover plate 507 is removed, the sampling robotic arm 3 starts its operation, moves to the sampling tip box 106, automatically picks up a brand new sampling tip, and completes the tip replacement, avoiding cross-contamination of samples caused by duplicate tips. After the tip replacement is completed, the sampling robotic arm 3 extends into the open culture dish 506 to collect samples from the cells inside. After sampling, the sampling robotic arm 3 moves above the sample box 105 and places the collected sample along with the used sampling tip into the sample box 105 to complete sample collection. After sample collection is completed, the drive mechanism 4 continues to operate, and the moving carriage 602 drives the negative pressure suction cup 607 and the adsorbed cover plate 507 to move back to the culture dish. Above the culture dish 506, the electric telescopic rod 606 extends again, pressing the cover plate 507 onto the mouth of the culture dish 506. Then, the negative pressure suction cup 607 releases the negative pressure adsorption, releasing the cover plate 507, which is stably closed on the culture dish 506, completing the complete process of opening, sampling, and closing the cover of a single culture dish 506. After completing a set of sampling, the drive mechanism 4 continues to operate, and the cylindrical pin 407 is inserted into the intermittent slot 503 again, driving the cross turntable 504 to complete the next indexing and rotation, transporting the next culture dish 506 to be sampled to the work station. The entire process of opening, sampling, and closing the cover is repeated in a cycle, realizing continuous automated batch sampling of multiple sets of culture dishes 506.

[0037] When the culture dish 506 completes the entire culture and sampling process and needs to be removed from the equipment, the cross-shaped turntable 504 continues to rotate intermittently. The guide pins 519 at the bottom of each moving slide bar 517 at the bottom of the cross-shaped turntable 504 slide along the annular guide groove 107 on the mounting base 1. The bottom height of most sections of the annular guide groove 107 remains unchanged, allowing the guide pins 519 to slide smoothly while the moving slide bars 517 remain in their original positions. When the guide pin 519 corresponding to the culture dish 506 slides to the raised section of the annular guide groove 107, the raised bottom of the groove lifts the guide pin 519, pushing the moving slide bar 517 horizontally along the mounting slide bar 516. The pushing protrusions 518 at both ends of the moving slide bar 517 then horizontally push the bottom of the L-shaped rotating bar 511. The L-shaped rotating bar 511 swings with the fixed protrusion 510 as the fulcrum, causing the L-shaped rotating bar to rotate. The upper end of strip 511 applies an upward pushing force to push block 514, causing concave top plate 512 to rise vertically upward along limiting slide post 509. Concave top plate 512 passes through the bottom of placement slot 505 and lifts culture dish 506 upward, so that culture dish 506 is lifted away from the limiting constraint of placement slot 505. Operators can directly remove culture dish 506 without manual prying, avoiding damage to culture dish 506. Cross turntable 504 continues to rotate, guide slide post 519 leaves the raised work position section, guide slide post 519 height returns, moving slide bar 517 resets, L-shaped rotating bar 511 swings in the opposite direction, concave top plate 512 falls back to reset, cross turntable 504 rotates to control the next work position culture dish 506 to rotate to the raised work position section of annular guide slide 107, repeat the lifting step of concave top plate 512, lift culture dish 506 one by one for removal.

Claims

1. An integrated cell culture and sampling device, characterized in that, include: Mounting base (1), a sealing shell (2) is fixedly installed at the top edge of the mounting base (1), the sealing shell (2) is arranged along the upper edge of the mounting base (1) to form a closed cell culture operation cavity, a sampling robotic arm (3) is fixedly installed on one side of the top of the mounting base (1), and the sampling robotic arm (3) is located inside the cavity enclosed by the sealing shell (2), a driving mechanism (4) is fixedly installed on the inner side of the mounting base (1), a workstation switching mechanism (5) is rotatably installed on the top of the mounting base (1) through a bearing, and a moving adsorption mechanism (6) is provided on the top of the mounting base (1) located inside the sealing shell (2) between the sampling robotic arm (3) and the workstation switching mechanism (5).

2. The integrated cell culture and sampling device according to claim 1, characterized in that, The mounting base (1) has a mounting groove 1 (101) at the bottom. The mounting base (1) has a mounting groove 2 (102) at the top of the mounting groove 1 (101) on the inner side. The top and bottom of the mounting groove 2 (102) are respectively provided with rectangular sliding grooves (103). A rectangular placement groove (104) is symmetrically provided at the edge of one side of the mounting base (1). A sample box (105) or a sampling gun head box (106) is respectively snapped into the rectangular placement groove (104). The top of the mounting base (1) is provided with an annular guide groove (107) at the position corresponding to the station switching mechanism (5).

3. The integrated cell culture and sampling device according to claim 2, characterized in that, The drive mechanism (4) includes a drive motor (401), which is fixedly connected to the bottom of the mounting groove (101). A drive bevel gear (402) is fixedly connected to the output end of the drive motor (401). A driven bevel gear (403) is rotatably mounted on the bottom of the mounting groove (101) on one side of the output end of the drive motor (401) via a bearing. The driven bevel gear (403) meshes with the drive bevel gear (402). The top of the driven bevel gear (403) is fixedly mounted on... A push crank shaft (404) is provided, and the top end of the push crank shaft (404) is rotatably connected to the top of the first mounting groove (101) through a bearing. The top end of the push crank shaft (404) extends through the first mounting groove (101) to the inside of the second mounting groove (102) and is fixedly connected to a mounting turntable (405). A dial (406) is fixedly installed on the top of the mounting turntable (405), and a cylindrical pin (407) is fixedly installed on the edge of the top of the mounting turntable (405) on one side of the dial (406).

4. The integrated cell culture and sampling device according to claim 3, characterized in that, The workstation switching mechanism (5) includes an intermittent turntable (501), and the top and bottom of the intermittent turntable (501) are rotatably connected to the inner side of the second mounting groove (102) by bearings. The outer circular surface of the intermittent turntable (501) is provided with four arc-shaped sliding grooves (502) at equal angles, and the arc-shaped sliding grooves (502) are close to the outer circular surface of the dial (406). The outer circular surface of the intermittent turntable (501) is provided with an intermittent slot (503) between two adjacent arc-shaped sliding grooves (502), and the intermittent slot (503) is correspondingly engaged with the cylindrical pin (407). The top of the intermittent turntable (501) extends through the second mounting groove (102) to the top of the mounting base (1) and is fixedly connected to a cross turntable (504), and the cross turntable (504) is close to the top of the mounting base (1).

5. The integrated cell culture and sampling device according to claim 4, characterized in that, The four corners of the cross turntable (504) are respectively provided with placement slots (505), and the placement slots (505) correspond to the positions of the arc-shaped slides (502). A petri dish (506) is placed inside the placement slot (505), and the top of the petri dish (506) is covered with a cover plate (507). The four corners of the cross turntable (504) are respectively provided with rectangular mounting slots (508) on both sides of the placement slots (505), and the inner walls of the rectangular mounting slots (508) are symmetrically fixedly installed with limit sliding columns (509). The side of the cross turntable (504) is fixedly connected to one side of the rectangular mounting slots (508), and an L-shaped rotating strip (511) is rotatably connected through the surface of the fixed protrusion (510).

6. The integrated cell culture and sampling device according to claim 5, characterized in that, The four corners of the cross turntable (504) are provided with concave top plates (512) at the bottom of the placement slot (505), and the two sides of the concave top plates (512) are slidably engaged with the inside of the rectangular mounting slot (508). The two sides of the concave top plates (512) are respectively provided with symmetrical limiting slide grooves (513), and the limiting slide grooves (513) are slidably connected to the surface of the limiting slide column (509). The center positions of the two sides of the concave top plates (512) are respectively fixedly connected with push top blocks (514), and one end of the L-shaped rotating bar (511) is slidably connected to the surface of the push top block (514) through the slide groove.

7. The integrated cell culture and sampling device according to claim 6, characterized in that, The bottom of the cross turntable (504) near the placement slot (505) is provided with a movable slide groove (515), and the inner wall of the movable slide groove (515) is symmetrically fixedly connected with a mounting slide column (516). The inner side of the movable slide groove (515) is slidably connected with a movable slide bar (517), and the movable slide bar (517) is slidably connected to the surface of the mounting slide column (516). The two ends of the movable slide bar (517) are respectively fixedly connected with a push protrusion (518), and the push protrusion (518) is slidably connected to the inside of the slide groove at the bottom of the L-shaped rotating bar (511). The center position of the bottom of the movable slide bar (517) is fixedly connected with a guide slide column (519), and the guide slide column (519) is slidably connected to the inside of the annular guide slide groove (107).

8. The integrated cell culture and sampling device according to claim 3, characterized in that, The movable adsorption mechanism (6) includes two limiting slides (601). The two limiting slides (601) are fixedly connected to the inner wall of the mounting groove (101), and the height of the limiting slides (601) corresponds to the height of the crank of the push crank shaft (404). A movable slide (602) is slidably connected through the surface between the two limiting slides (601). A push groove (603) is provided through the center of the movable slide (602), and the crank of the push crank shaft (404) is slidably connected through the inside of the push groove (603). A rectangular connecting plate (604) is fixedly connected to the top of each end, and the rectangular connecting plate (604) is slidably connected inside the rectangular slide groove (103). The top of the rectangular connecting plate (604) extends through the rectangular slide groove (103) to the top of the mounting base (1). A mounting connecting plate (605) is fixedly connected between the tops of the two rectangular connecting plates (604) on opposite sides. An electric telescopic rod (606) is embedded and fixedly installed at the center of the top of the mounting connecting plate (605), and the telescopic end of the top of the electric telescopic rod (606) extends through the mounting connecting plate (605) to the bottom of the mounting connecting plate (605) and is fixedly connected to a negative pressure suction cup (607).

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