Stem cell culture device and culture method thereof

Abstract

The application belongs to the technical field of stem cell culture, and particularly relates to a stem cell culture device and a culture method thereof, which comprises a culture box and a test tube tray arranged in the inner cavity of the culture box, a plurality of test tube holes are arranged on the test tube tray, and an automatic test tube hole returning mechanism is arranged in the culture box; the automatic test tube hole returning mechanism comprises a turnable placing frame and a vibration assembly capable of generating vibration of the placing frame, a plurality of horizontal rods are fixedly connected to the bottom of the placing frame in the transverse direction, horizontal sliding grooves are arranged on the horizontal rods, and a plurality of plug-in plates are slidably inserted into the sliding grooves in the transverse direction. The automatic test tube hole returning mechanism is used, and after each culture work is completed, the cleaned test tubes can be automatically returned to the test tube holes, manual arrangement and insertion of the test tubes are not needed in the whole process, the labor intensity is greatly reduced, and the automatic test tube returning process is realized.

Description

Technical Field

[0001] This invention belongs to the field of stem cell culture technology, specifically a stem cell culture device and its culture method. Background Technology

[0002] Stem cells are a type of primitive cell with the potential for self-renewal and differentiation, and they also have potential pluripotency, capable of differentiating into various cell types, including muscle, nerve, bone, and blood. Therefore, stem cells have broad application prospects in the fields of medicine and biotechnology, including disease treatment, drug development, and regenerative medicine. In response to the large demand in different fields, the demand for stem cell culture is gradually increasing, and stem cell culture devices are required in the process of cultivation.

[0003] In existing technologies, when culturing stem cells, multiple test tubes are usually inserted into the wells of each test tube first, then the stem cells and culture medium are added to the test tubes, and finally the temperature and humidity of the environment in which the stem cells are located are controlled to culture the stem cells in a suitable environment.

[0004] However, after each culture cycle, the test tubes need to be removed from the wells and cleaned to prepare for the next cycle. After cleaning, operators must manually place multiple tubes back into the wells one by one, resulting in high labor intensity. Furthermore, even if a robotic arm could be used to replace manual placement, the randomly stacked tubes in various postures require the robotic arm's vision system to spend considerable time on target recognition, posture calculation, and path planning in three-dimensional space. The high light transmittance of the test tubes and potential residual water can cause glare or image distortion, leading to a very high failure rate in grasping, making it difficult to meet the cycle time requirements of high-throughput cultures. Summary of the Invention

[0005] In order to overcome the shortcomings of the prior art and solve at least one of the technical problems mentioned in the background art, the present invention proposes a stem cell culture device and a culture method thereof.

[0006] The technical solution adopted by the present invention to solve its technical problem is: a stem cell culture device, including a culture box and a test tube tray disposed in the inner cavity of the culture box, the test tube tray having multiple rows of test tube holes, and the culture box having an automatic test tube hole return mechanism inside; The automatic test tube return mechanism includes a flip-up placement frame and a vibration component that can make the placement frame vibrate. Multiple horizontal bars are fixedly connected to the bottom of the placement frame in the horizontal direction. The horizontal bars are provided with horizontal grooves, and plug plates are slidably inserted into the grooves in the horizontal direction. The horizontal bars and plug plates cooperate with each other to form multiple strip channels that are adapted to the size of the test tube. The automatic test tube return mechanism also includes a spacing adjustment component; The pitch adjustment assembly includes multiple leaf springs arranged at equal intervals along the lateral direction, and a displacement structure for driving the multiple leaf springs to move simultaneously.

[0007] Preferably, the vibration assembly includes two flip plates symmetrically distributed on both sides of the placement frame. Multiple spring dampers are fixedly mounted on the flip plates. The placement frame is fixedly mounted on the piston end of the spring dampers. An electromagnetic vibrator is mounted on the placement frame.

[0008] Preferably, the incubator has supports fixedly installed on both sides of its inner cavity, and two rotating plates are respectively mounted on the supports on both sides. A motor is fixedly installed on the supports, and the output end of the motor is fixedly connected to one end of the rotating plate.

[0009] Preferably, the incubator is provided with a lead screw slide rail module one, a transverse plate is fixedly provided at the slider of the lead screw slide rail module one, multiple guide rods slide vertically through the transverse plate, the test tube tray is fixedly provided on the guide rods, and a cylinder three is fixedly provided on the transverse plate, the piston end of the cylinder three is fixedly connected to one side of the test tube tray.

[0010] Preferably, an adjustment frame is provided on the placement frame along the horizontal direction, and multiple plug-in plates are fixedly connected to the adjustment frame. A second cylinder is fixedly provided on the placement frame, and the piston end of the second cylinder is fixedly connected to one end of the adjustment frame.

[0011] Preferably, the displacement structure includes a second lead screw slide rail module mounted on a placement frame. The movable end of the second lead screw slide rail module is fixedly mounted on a support frame. A linear slide rail module is mounted on the support frame. A lifting plate is fixedly mounted on the movable end of the linear slide rail module. A variable pitch slide is slidably mounted on the bottom of the lifting plate. Multiple sliders with variable spacing are mounted on the variable pitch slide along the horizontal direction. A fixed rod is fixedly connected to the bottom of each slider. Each leaf spring is fixedly connected to the bottom of a fixed rod. A fourth cylinder is fixedly mounted on the lifting plate. The piston end of the fourth cylinder is fixedly connected to one end of the variable pitch slide.

[0012] Preferably, the incubator is equipped with an oxygen inlet pipe and a carbon dioxide inlet pipe, and the oxygen inlet pipe and the carbon dioxide inlet pipe are respectively equipped with an oxygen metering pump and a carbon dioxide metering pump. The inner wall of the incubator is equipped with a temperature sensor, a humidity sensor, a carbon dioxide concentration sensor and an electric heating plate.

[0013] Preferably, a threaded rod is rotatably provided on one side of the test tube tray, and multiple bidirectional threads are intermittently provided on the threaded rod. Two positioning plates are threadedly connected to each bidirectional thread, and the two positioning plates are respectively located on the two helical sections on both sides of the bidirectional thread. The positioning plates are slidably disposed on the test tube tray in the transverse direction. A second motor is fixedly provided on the test tube tray, and the output end of the second motor is fixedly connected to one end of the threaded rod.

[0014] Preferably, the placement frame is provided with an anti-detachment component; The anti-detachment component includes a screw slide rail module three mounted on the placement frame. A cylinder one is fixedly mounted on the moving end of the screw slide rail module three. An adsorption plate is fixedly connected to the piston end of the cylinder one. The adsorption plate has multiple grooves, and each groove has several air holes. An air pump is mounted on the bracket. The air pump inlet is connected to the inner cavity of the adsorption plate through a hose.

[0015] A method for culturing stem cells specifically includes the following steps: S1. The adjusting frame moves horizontally, causing the insertion plate and crossbar to cooperate to form multiple strip channels; S2. Multiple test tubes are placed into the placement frame. Under the action of the electromagnetic vibrator and spring damper, the test tubes vibrate and move until they all pass through the strip channels and are in a vertical state with the tube openings facing upwards; S3. The strip channels tilt, and the test tubes slide along the channels by gravity until they fit together. Then the placement frame returns to a horizontal position; S4. The variable pitch slide table moves multiple fixed rods and aligns them with the axis of each test tube. Multiple leaf springs extend into the test tubes to fix them. Then the distance between adjacent leaf springs is adjusted to match the distance between the test tubes and the distance between the test tube holes; S5. The test tube tray rises, and the test tubes are inserted into the corresponding test tube holes. Adjacent positioning plates close together to clamp a row of test tubes. The insertion plate retracts, and the test tubes are removed from the strip channels; S6. Remove the test tube tray and add stem cells and culture medium to each test tube; during culture, carbon dioxide metering pump introduces carbon dioxide through carbon dioxide inlet pipe, and the concentration is detected by carbon dioxide concentration sensor; oxygen metering pump introduces oxygen through oxygen inlet pipe; temperature sensor and humidity sensor monitor the temperature and humidity inside the incubator.

[0016] The beneficial effects of this invention are as follows: 1. The stem cell culture device and method described in this invention utilize an automatic test tube return mechanism. After each culture cycle, multiple cleaned test tubes are automatically returned to their respective wells, eliminating the need for manual arrangement and insertion of test tubes by operators, significantly reducing labor intensity and automating the test tube return process. Simultaneously, this solution leverages the structural characteristics of the test tubes themselves, using vibration-assisted material handling and strip channel constraints to automatically arrange randomly stacked test tubes into a uniform vertical posture with the tube openings facing upwards. This completely avoids the recognition difficulties and grasping failures caused by the high light transmittance of test tubes, water stain reflections, and random stacking postures of robotic vision systems. Furthermore, through the cooperation of a variable-pitch slide and leaf springs, the tubes are first adaptively arranged at equal intervals based on their diameter, and then a second pitch adjustment is performed according to the spacing between the test tube wells. This allows for the grasping and precise alignment of an entire row in one operation, significantly improving the tube placement cycle time and meeting the efficiency requirements of high-throughput culture. Finally, the positioning plate on the test tube tray clamps the inserted test tubes, ensuring the stability of the test tubes during the culture process, making the entire process from cleaning to repacking and then to culture seamless, stable and reliable.

[0017] 2. The stem cell culture device and method described in this invention utilize an anti-detachment component. While the placement frame vibrates, an air pump is activated, creating negative pressure at the pores. At this time, the test tubes attached to the adsorption plate naturally cover part of the pores, thus being firmly adsorbed and fixed. In this way, the positioned test tubes will not detach again during subsequent vibrations, ensuring that all test tubes can quickly and simultaneously fall into the strip channel and be arranged in place, significantly shortening the material handling time and improving work efficiency. Attached Figure Description

[0018] The invention will now be further described with reference to the accompanying drawings.

[0019] Figure 1 This is a three-dimensional structural schematic diagram of the present invention; Figure 2 This is a schematic diagram of the internal three-dimensional structure of the incubator; Figure 3 This is a schematic diagram of the three-dimensional structure of the electric heating plate; Figure 4 This is a schematic diagram of the three-dimensional structure at the placement frame; Figure 5 yes Figure 4 Enlarged view of a portion of point A in the middle; Figure 6 This is a schematic diagram of the three-dimensional structure from another perspective of the placement frame; Figure 7 This is a schematic diagram of the internal three-dimensional structure of the placement frame; Figure 8 yes Figure 7 Enlarged view of a section at point B in the middle; Figure 9 This is a schematic diagram of the three-dimensional structure of the adsorption plate. Figure 10 This is a schematic diagram of the three-dimensional structure of the test tube tray. Figure 11 yes Figure 10 Enlarged view of a section at point C.

[0020] In the diagram: 1. Incubator; 2. Oxygen inlet pipe; 3. Carbon dioxide inlet pipe; 4. Support frame; 5. Screw and slide rail module one; 6. Temperature sensor; 7. Humidity sensor; 8. Carbon dioxide concentration sensor; 9. Electric heating plate; 10. Horizontal sliding plate; 11. Test tube tray; 12. Motor one; 13. Tilting plate; 14. Spring damper; 15. Electromagnetic vibrator; 16. Placement frame; 17. Air pump; 18. Guide rod; 19. Test tube hole; 20. 21. Positioning plate; 22. Threaded rod; 23. Stand; 24. Screw slide rail module two; 25. Screw slide rail module three; 26. Adsorption plate; 27. Cylinder one; 28. Hose; 29. ​​Adjusting frame; 30. Cylinder two; 31. Lifting plate; 32. Variable pitch slide table; 33. Linear slide rail module; 34. Fixed rod; 35. Leaf spring; 36. Crossbar; 37. Cylinder four; 38. Air hole; 39. Plug-in plate; 40. Cylinder three; 41. Motor two. Detailed Implementation

[0021] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] Please refer to Figures 1-11 The present invention provides a technical solution: a stem cell culture device, including a culture box 1 and a test tube tray 11 disposed in the inner cavity of the culture box 1, the test tube tray 11 having multiple rows of test tube holes 19, and the culture box 1 having an automatic test tube hole return mechanism inside. The automatic test tube return mechanism includes a flip-up placement frame 16 and a vibration component that can cause the placement frame 16 to vibrate. Multiple horizontal bars 35 are fixedly connected to the bottom of the placement frame 16 in the horizontal direction. The horizontal bars 35 are provided with horizontal grooves, and the insertion plates 38 are slidably inserted into the grooves in the horizontal direction. The horizontal bars 35 and the insertion plates 38 cooperate with each other to form multiple strip channels that are adapted to the size of the test tube. The automatic test tube return mechanism also includes a spacing adjustment component; The pitch adjustment assembly includes multiple leaf springs 34 arranged equidistantly in the transverse direction, and a displacement structure for driving the multiple leaf springs 34 to move simultaneously.

[0023] In this embodiment, as Figures 2-11 As shown, the vibration assembly includes two flip plates 13, which are symmetrically distributed on both sides of the placement frame 16. Multiple spring dampers 14 are fixed on the flip plates 13. The placement frame 16 is fixed on the piston end of the spring dampers 14. An electromagnetic vibrator 15 is provided on the placement frame 16.

[0024] The incubator 1 has two fixed supports 4 on both sides of its inner cavity. Two rotating plates 13 are respectively mounted on the supports 4 on both sides. A motor 12 is fixed on the support 4, and the output end of the motor 12 is fixedly connected to one end of the rotating plate 13.

[0025] The incubator 1 is equipped with a screw slide rail module 5. A transverse plate 10 is fixed at the slider of the screw slide rail module 5. Multiple guide rods 18 slide vertically through the transverse plate 10. The test tube tray 11 is fixed on the guide rods 18. A cylinder 39 is fixed on the transverse plate 10. The piston end of the cylinder 39 is fixedly connected to one side of the test tube tray 11.

[0026] An adjustment frame 28 is provided on the horizontal sliding side of the placement frame 16. Multiple plug-in plates 38 are fixedly connected to the adjustment frame 28. A cylinder 29 is fixedly provided on the placement frame 16. The piston end of the cylinder 29 is fixedly connected to one end of the adjustment frame 28.

[0027] The displacement structure includes a second lead screw slide rail module 23 mounted on a placement frame 16. A support frame 22 is fixedly mounted on the moving end of the second lead screw slide rail module 23. A linear slide rail module 32 is mounted on the support frame 22. A lifting plate 30 is fixedly mounted on the moving end of the linear slide rail module 32. A variable pitch slide table 31 is slidably mounted on the bottom of the lifting plate 30 along the horizontal direction. Multiple sliders with variable spacing are mounted on the variable pitch slide table 31 along the horizontal direction. A fixed rod 33 is fixedly connected to the bottom of each slider. Each leaf spring 34 is fixedly connected to the bottom of a fixed rod 33. A fourth cylinder 36 is fixedly mounted on the lifting plate 30. The piston end of the fourth cylinder 36 is fixedly connected to one end of the variable pitch slide table 31.

[0028] The incubator 1 is equipped with an oxygen inlet pipe 2 and a carbon dioxide inlet pipe 3. The oxygen inlet pipe 2 and the carbon dioxide inlet pipe 3 are respectively equipped with an oxygen metering pump and a carbon dioxide metering pump. The inner wall of the incubator 1 is equipped with a temperature sensor 6, a humidity sensor 7, a carbon dioxide concentration sensor 8, and an electric heating plate 9.

[0029] A threaded rod 21 is rotatably provided on one side of the test tube tray 11. The threaded rod 21 has multiple bidirectional threads intermittently provided. Each bidirectional thread is threaded with two positioning plates 20. The two positioning plates 20 are located on the two sides of the spiral section of the bidirectional thread, respectively. The positioning plates 20 are slidably provided on the test tube tray 11. A second motor 40 is fixedly provided on the test tube tray 11. The output end of the second motor 40 is fixedly connected to one end of the threaded rod 21.

[0030] Specifically, this embodiment is used to place test tubes of the same specifications back into the test tube hole 19 in a single application. The test tubes targeted in this embodiment are plastic test tubes. The test tubes have a raised edge at the opening, which makes it easy for people to hold or place them. This is a conventional design for test tubes. Therefore, the diameter of the test tube is larger than the diameter of its main body. Using this structural feature, the adjustment work is carried out first.

[0031] First, the cylinder 29 drives the adjusting frame 28 to move laterally on the placement frame 16, and the multiple plug-in plates 38 will slide in each groove. Since the crossbar 35 and the plug-in plates 38 cooperate to form a strip channel, the width of the strip channel can be controlled in this way, and the width can be made between the diameter of the test tube opening and the diameter of the test tube body.

[0032] After completing the above adjustments, place the multiple test tubes to be used into the placement frame 16. Then, start the electromagnetic vibrator 15. With the cooperation of the electromagnetic vibrator 15 and the spring damper 14, the placement frame 16 vibrates, causing the multiple test tubes to move continuously within the placement frame 16. Since the width of the strip channel is between the diameter of the test tube opening and the diameter of the test tube body, the tube opening cannot pass through the strip channel; only the body of the test tube can pass through. In this way, as the placement frame 16 continues to vibrate, all the test tubes will eventually pass through the strip channel, and the tube openings will be stuck at the insertion plate 38, with all the test tubes in a vertical position with the tube openings facing upwards.

[0033] After all the test tubes have fallen into their respective strip channels, the electromagnetic vibrator 15 is turned off. Then, motor 12 drives the placement frame 16 to rotate at a certain angle, tilting the strip channels. Multiple test tubes in the strip channels will slide along the channels under gravity until they are in contact with each other. Then, the placement frame 16 is returned to a horizontal position. At this point, the distance between the axes of two adjacent test tubes is equal to the diameter of the test tube. Based on this characteristic, cylinder 36 drives the variable-pitch slide 31 to slide on the lifting plate 30, aligning the fixed rod 33 closest to the upright 22 with the axis of the test tube closest to the upright 22 in the strip channel. Then, based on the distance between the axes of two adjacent test tubes, the variable-pitch slide 31 is activated. Multiple sliders on the variable-pitch slide 31 simultaneously move multiple fixed rods 33, aligning the remaining fixed rods 33 with the axes of the remaining test tubes. Subsequently, the linear guide module 32 drives the lifting plate 30 to descend, causing multiple leaf springs 34 to simultaneously extend into multiple test tubes. Because the leaf springs 34 are V-shaped, they are compressed by the tube wall as they enter through the tube opening. Once fully inserted, they press tightly against the tube wall under the action of elasticity, thus fixing the test tube in place. At this point, the distance between adjacent leaf springs 34 can be adjusted according to the spacing of adjacent test tube holes 19 on the test tube tray 11, thus changing the spacing between adjacent test tubes to match the spacing between two adjacent test tube holes 19. Then, the insertion plate 38 clamps the row of test tubes whose spacing has been adjusted, and the leaf springs 34 extend out of the tubes.

[0034] Repeating the above operation, the spacing between adjacent test tubes in multiple strip channels can be adjusted by using the screw guide module 23 and the linear guide module 32 to adjust the orientation of the variable pitch slide table 31 in the horizontal and vertical directions. Furthermore, since one edge of each row of test tube holes 19 is aligned with one edge of a strip channel, when the spacing between adjacent test tubes in the strip channel is equal to the spacing between adjacent test tube holes 19, each test tube is also aligned with a test tube hole 19. At this time, the test tube tray 11 is raised by the cylinder 39, and each test tube is inserted into a test tube hole 19. Next, the motor 40 drives the threaded rod 21 to rotate. Through the multiple bidirectional threads on the threaded rod 21, the two adjacent positioning plates 20 are brought closer together, simultaneously clamping multiple test tubes in a row of test tube holes 19 to ensure the stability of the test tubes. At this time, the insertion plate 38 moves away from the test tube, allowing the test tube to detach from the strip channel.

[0035] Then, using the lead screw and slide rail module 5, the test tube tray 11 is moved away from below the placement frame 16. At this point, the operator can add stem cells and culture medium to each test tube for culture. During the culture process, external carbon dioxide is introduced into the carbon dioxide inlet pipe 3 through a carbon dioxide metering pump, and the carbon dioxide concentration in the incubator 1 is detected by the carbon dioxide concentration sensor 8, which helps to ensure that the carbon dioxide concentration in the incubator 1 is within the normal range. External oxygen is introduced into the oxygen inlet pipe 2 through an oxygen metering pump, and then oxygen is introduced into the incubator 1 through the oxygen inlet pipe 2, which helps to ensure the oxygen content in the incubator 1. The temperature and humidity in the incubator 1 are measured by the temperature sensor 6 and the humidity sensor 7, and the interior of the incubator 1 is heated by the electric heating plate 9. In summary, this helps to control the culture environment in the incubator 1 to culture stem cells, thereby ensuring the normal culture of stem cells.

[0036] Thus, through the above operations, after each culture cycle, multiple cleaned test tubes can be automatically returned to their respective wells 19, eliminating the need for manual arrangement and placement of test tubes by operators, significantly reducing labor intensity and automating the test tube return process. Simultaneously, this solution utilizes the structural characteristics of the test tubes themselves, employing vibration-assisted material handling and strip channel constraints to automatically arrange randomly stacked test tubes into a uniform vertical posture with the tube openings facing upwards. This completely avoids the recognition difficulties and grasping failures caused by the high light transmittance of test tubes, water stain reflections, and random stacking postures of robotic vision systems. Furthermore, through the cooperation of the variable-pitch slide 31 and the leaf spring 34, the tubes are first adaptively arranged at equal intervals based on their diameter, and then a second pitch adjustment is performed according to the spacing between the test tube wells. This allows for the precise grasping and alignment of an entire row in one operation, significantly improving the tube placement cycle time and meeting the efficiency requirements of high-throughput culture. Finally, the positioning plate 20 on the test tube tray 11 clamps the inserted test tube, ensuring the stability of the test tube during the culture process, making the entire process from cleaning to repacking and then to culture seamless, stable and reliable.

[0037] In this embodiment, as Figure 8 and Figure 9 As shown, the placement frame 16 is equipped with an anti-detachment component; The anti-detachment component includes a screw slide rail module 24 mounted on the placement frame 16. The moving end of the screw slide rail module 24 is fixedly equipped with a cylinder 26. The piston end of the cylinder 26 is fixedly connected to an adsorption plate 25. The adsorption plate 25 has multiple grooves, and each groove has several air holes 37. The bracket 4 is equipped with an air pump 17. The air inlet of the air pump 17 is connected to the inner cavity of the adsorption plate 25 through a hose 27.

[0038] Specifically, in the above embodiments, although vibration can force the test tubes to pass through the strip channel, the test tubes that have already passed through are prone to falling out again under continuous vibration, thus prolonging the time it takes for all the test tubes to be in place and affecting work efficiency. To solve this problem, the working principle of this embodiment is as follows: Each groove on the adsorption plate 25 corresponds to a strip channel. Based on the test tube length, cylinder 26 raises and lowers the adsorption plate 25, allowing the test tube to pass through the strip channel so that its bottom fits precisely against the surface of the adsorption plate 25. Simultaneously with the vibration of the placement frame 16, the air pump 17 is activated, creating negative pressure at the air pores 37. At this time, the test tubes attached to the adsorption plate 25 naturally cover part of the air pores 37, thus being firmly adsorbed and fixed. In this way, the positioned test tubes will not fall out again during subsequent vibrations, ensuring that all test tubes can quickly and in one go fall into the strip channel and be arranged in place, significantly shortening the material handling time and improving work efficiency.

[0039] Furthermore, once all the test tubes are in place, the suction plate 25 is moved laterally by the screw slide rail module 24, so that it is moved away from below the strip channel without affecting the normal insertion of the test tubes into the test tube hole 19.

[0040] A method for culturing stem cells specifically includes the following steps: S1. The adjusting frame 28 moves horizontally, causing the plug-in plate 38 and the crossbar 35 to cooperate to form multiple strip channels; S2. Multiple test tubes are placed into the placement frame 16. Under the action of the electromagnetic vibrator 15 and the spring damper 14, the test tubes vibrate and move until they all pass through the strip channels and are in a vertical state with the tube openings facing upwards; S3. The strip channels tilt, and the test tubes slide along the channels by gravity until they fit together. Then the placement frame 16 returns to a horizontal position; S4. The variable pitch slide table 31 drives multiple fixed rods 33 to move and align with the axis of each test tube. Multiple leaf springs 34 extend into the test tubes to fix them. Then the distance between adjacent leaf springs 34 is adjusted so that the distance between the test tubes matches the distance between the test tube holes 19; S5. The test tube tray 11 rises, and the test tubes are inserted into the corresponding test tube holes 19. The adjacent positioning plates 20 move together to clamp a row of test tubes. The plug-in plate 38 is withdrawn, and the test tubes are removed from the strip channels. S6. Remove the test tube tray 11 and add stem cells and culture medium to each test tube; during culture, carbon dioxide metering pump introduces carbon dioxide through carbon dioxide inlet pipe 3, and the concentration is detected by carbon dioxide concentration sensor 8; oxygen metering pump introduces oxygen through oxygen inlet pipe 2; temperature sensor 6 and humidity sensor 7 monitor the temperature and humidity inside incubator 1.

[0041] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A stem cell culture device, comprising an incubator (1) and a test tube tray (11) disposed within the cavity of the incubator (1), wherein the test tube tray (11) has multiple rows of test tube holes (19), characterized in that: The incubator (1) is equipped with an automatic test tube return mechanism; The automatic test tube return mechanism includes a flip-up placement frame (16) and a vibration component that can make the placement frame (16) vibrate. The bottom of the placement frame (16) is fixedly connected with multiple horizontal bars (35). The horizontal bars (35) are provided with horizontal grooves, and the grooves are slidably inserted with plug plates (38). The horizontal bars (35) and plug plates (38) cooperate with each other to form multiple strip channels that are adapted to the size of the test tube. The automatic test tube return mechanism also includes a spacing adjustment component; The pitch adjustment assembly includes multiple leaf springs (34) arranged equidistantly in the transverse direction, and a displacement structure for driving the multiple leaf springs (34) to move simultaneously.

2. The stem cell culture device according to claim 1, characterized in that: The vibration assembly includes two flip plates (13), which are symmetrically distributed on both sides of the placement frame (16). Multiple spring dampers (14) are fixed on the flip plates (13). The placement frame (16) is fixed on the piston end of the spring damper (14). An electromagnetic vibrator (15) is provided on the placement frame (16).

3. The stem cell culture device according to claim 2, characterized in that: The incubator (1) has a bracket (4) fixed on both sides of its inner cavity. Two flip plates (13) are respectively rotated on the brackets (4) on both sides. A motor (12) is fixed on the bracket (4). The output end of the motor (12) is fixedly connected to one end of the flip plate (13).

4. The stem cell culture device according to claim 3, characterized in that: The incubator (1) is provided with a screw slide rail module (5), and a transverse plate (10) is fixedly provided at the slider of the screw slide rail module (5). Multiple guide rods (18) slide vertically through the transverse plate (10). The test tube tray (11) is fixedly provided on the guide rods (18). A cylinder (39) is fixedly provided on the transverse plate (10). The piston end of the cylinder (39) is fixedly connected to one side of the test tube tray (11).

5. A stem cell culture device according to claim 4, characterized in that: An adjustment frame (28) is provided on the horizontal side of the placement frame (16). Multiple plug-in plates (38) are fixedly connected to the adjustment frame (28). A cylinder (29) is fixedly provided on the placement frame (16). The piston end of the cylinder (29) is fixedly connected to one end of the adjustment frame (28).

6. The stem cell culture device according to claim 1, characterized in that: The displacement structure includes a second screw slide rail module (23) mounted on a placement frame (16). The moving end of the second screw slide rail module (23) is fixedly provided with a stand (22). A linear slide rail module (32) is mounted on the stand (22). A lifting plate (30) is fixedly provided on the moving end of the linear slide rail module (32). A variable pitch slide table (31) is provided at the bottom of the lifting plate (30) along the horizontal direction. A plurality of sliders with variable spacing are provided on the variable pitch slide table (31) along the horizontal direction. A fixed rod (33) is fixedly connected to the bottom of each slider. Each leaf spring (34) is fixedly connected to the bottom of a fixed rod (33). A cylinder (36) is fixedly provided on the lifting plate (30). The piston end of the cylinder (36) is fixedly connected to one end of the variable pitch slide table (31).

7. A stem cell culture device according to claim 6, characterized in that: The incubator (1) is equipped with an oxygen inlet pipe (2) and a carbon dioxide inlet pipe (3). The oxygen inlet pipe (2) and the carbon dioxide inlet pipe (3) are respectively equipped with an oxygen metering pump and a carbon dioxide metering pump. The inner wall of the incubator (1) is equipped with a temperature sensor (6), a humidity sensor (7), a carbon dioxide concentration sensor (8), and an electric heating plate (9).

8. A stem cell culture device according to claim 2, characterized in that: The test tube tray (11) is provided with a threaded rod (21) on one side. The threaded rod (21) is provided with multiple bidirectional threads intermittently. Each bidirectional thread is threaded with two positioning plates (20). The two positioning plates (20) are located on the two sides of the spiral section of the bidirectional thread. The positioning plates (20) are slidably disposed on the test tube tray (11) in the transverse direction. The test tube tray (11) is fixedly provided with a second motor (40). The output end of the second motor (40) is fixedly connected to one end of the threaded rod (21).

9. A stem cell culture device according to claim 3, characterized in that: The placement frame (16) is provided with an anti-detachment component; The anti-detachment component includes a screw slide rail module three (24) mounted on the placement frame (16). The moving end of the screw slide rail module three (24) is fixedly equipped with a cylinder one (26). The piston end of the cylinder one (26) is fixedly connected to an adsorption plate (25). The adsorption plate (25) is provided with multiple grooves, and each groove is provided with several air holes (37). The bracket (4) is equipped with an air pump (17). The air inlet end of the air pump (17) is connected to the inner cavity of the adsorption plate (25) through a hose (27).

10. A method for culturing stem cells, characterized in that, The cultivation using the culture apparatus as described in any one of claims 1-9 specifically includes the following steps: S1. The adjusting frame (28) moves laterally, causing the plug plate (38) and the crossbar (35) to cooperate to form multiple strip channels; S2. Place multiple test tubes into the placement frame (16). Under the action of the electromagnetic vibrator (15) and the spring damper (14), the test tubes vibrate and move until they all pass through the strip channel and are in a vertical state with the tube opening facing upwards. S3, the strip channel is tilted, and the test tubes slide along the channel by gravity until they fit together. Then the frame (16) is placed back to horizontal. S4. The variable pitch slide (31) drives multiple fixed rods (33) to move and align with the axis of each test tube. Multiple leaf springs (34) extend into the test tube to fix it. Then adjust the distance between adjacent leaf springs (34) to make the distance between test tubes match the distance between test tube holes (19). S5. The test tube tray (11) rises, the test tubes are inserted into the corresponding test tube holes (19), the adjacent positioning plates (20) move closer to clamp a row of test tubes, the plug plate (38) is removed, and the test tubes are removed from the strip channel; S6. The test tube tray (11) is removed, and stem cells and culture medium are added to each test tube; During culture, carbon dioxide metering pump introduces carbon dioxide through carbon dioxide inlet pipe (3), and the concentration is detected by carbon dioxide concentration sensor (8); oxygen metering pump introduces oxygen through oxygen inlet pipe (2); temperature sensor (6) and humidity sensor (7) monitor the temperature and humidity inside the incubator (1).

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