Multi-medium biological toxicity integrated detection method and related device

Abstract

The application provides a multi-medium biological toxicity integrated detection method and related device, the method comprises the following steps: pretreating a first sample according to a first sample type corresponding to the first sample to obtain a liquid to-be-tested sample; quantitatively adding the liquid to-be-tested sample and a liquid control sample to a sample detection cavity and a control detection cavity of a first detection tube; synchronously performing bacteria liquid mixing and biochemical reaction on the liquid to-be-tested sample and the liquid control sample to obtain a first target mixed sample and a second target mixed sample; synchronously performing detection operation on the first target mixed sample and the second target mixed sample to obtain first luminescence intensity data and second luminescence intensity data; and determining a first detection result of the first sample according to the first luminescence intensity data and the second luminescence intensity data. The multi-medium sample is uniformly converted into a liquid sample through differential pretreatment, and is automatically detected through an integrated process, so that the detection efficiency of the multi-medium biological toxicity is improved.

Description

Technical Field

[0001] This application relates to the field of toxicity detection technology, and in particular to an integrated method and device for multi-media biological toxicity detection. Background Technology

[0002] In the field of integrated environmental management and risk emergency response, biotoxicity testing of multiple media, including water, soil, air, and solids, is a key means of assessing the degree of harm caused by environmental pollutants. However, existing testing equipment is usually only compatible with a single medium and cannot be adapted to four different forms of media, including water, soil, air, and solids. Furthermore, the pretreatment processes for different media vary significantly, and there is a lack of integrated solutions. This results in low testing efficiency and long testing cycles in multi-media complex pollution scenarios, making it difficult to achieve rapid on-site testing.

[0003] Therefore, improving the detection efficiency of multi-media biotoxicity is an urgent problem to be solved. Summary of the Invention

[0004] This application provides a multi-media biotoxicity integrated detection method and related device. By performing differentiated pretreatment on samples from different media to uniformly convert them into liquid test samples, and using an integrated dual-chamber detection tube to simultaneously perform bacterial mixing, biochemical reaction and luminescence detection on the liquid test samples and liquid control samples, the sample detection results are determined based on the detection data, thereby improving the detection efficiency of multi-media biotoxicity.

[0005] In a first aspect, embodiments of this application provide an integrated detection method for multi-media biotoxicity, the method comprising: The first sample is preprocessed according to the first sample type corresponding to the first sample to obtain a liquid sample to be tested; the first sample is any one of the multi-media samples that have been collected, and the multi-media samples include at least one of the following: water sample, gaseous sample, soil sample, and solid waste sample. The liquid sample to be tested and the preset liquid control sample are quantitatively added to the sample detection chamber and control detection chamber of the first detection tube, respectively; the first detection tube is an integrated dual-chamber detection tube with the sample detection chamber and the control detection chamber being independent of each other; The liquid test sample and the liquid control sample were simultaneously mixed with bacterial solution and subjected to biochemical reaction to obtain the first target mixed sample and the second target mixed sample, respectively. For the first target mixed sample and the second target mixed sample, detection operations are performed simultaneously to obtain the first luminescence intensity data and the second luminescence intensity data, respectively; Based on the first luminescence intensity data and the second luminescence intensity data, the first detection result of the first sample is determined.

[0006] Secondly, embodiments of this application provide an integrated multi-media biotoxicity detection device, the device comprising a pretreatment module, a sample addition module, a mixing module, a detection module, and a determination module, wherein: The preprocessing module is used to preprocess the first sample according to the first sample type corresponding to the first sample to obtain a liquid sample to be tested; the first sample is any one of the collected multi-media samples, and the multi-media samples include at least one of the following: water sample, gaseous sample, soil sample, and solid waste sample. The sample addition module is used to quantitatively add the liquid test sample and the preset liquid control sample to the sample detection chamber and control detection chamber of the first detection tube, respectively; the first detection tube is an integrated dual-chamber detection tube with mutually independent sample detection chamber and control detection chamber; The mixing module is used to simultaneously mix the liquid test sample and the liquid control sample with bacterial solution and perform biochemical reactions to obtain a first target mixed sample and a second target mixed sample, respectively. The detection module is used to simultaneously perform detection operations on the first target mixed sample and the second target mixed sample to obtain first luminescence intensity data and second luminescence intensity data, respectively. The determining module is used to determine the first detection result of the first sample based on the first luminescence intensity data and the second luminescence intensity data.

[0007] Thirdly, embodiments of this application provide an electronic device, including a processor, a memory, a communication interface, and one or more programs, wherein the one or more programs are stored in the memory and configured to be executed by the processor, and the programs include instructions for performing steps in any method of the first aspect of this application.

[0008] Fourthly, embodiments of this application provide a computer-readable storage medium storing a computer program for electronic data interchange, wherein the computer program causes a computer to perform some or all of the steps described in any method of the first aspect of this application.

[0009] Fifthly, embodiments of this application provide a computer program product, wherein the computer program product includes a non-transitory computer-readable storage medium storing a computer program operable to cause a computer to perform some or all of the steps described in any method of the first aspect of this application. The computer program product may be a software installation package.

[0010] It is evident that by performing differentiated pretreatment on samples from different media to uniformly convert them into liquid test samples, and using an integrated dual-lumen detection tube to simultaneously perform bacterial mixing, biochemical reactions, and luminescence detection on the liquid test samples and liquid control samples, and then determining the sample test results based on the detection data, the detection efficiency of multi-media biotoxicity is improved. Attached Figure Description

[0011] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0012] Figure 1 This is a system architecture diagram of an intelligent integrated detection system provided in an embodiment of this application; Figure 2 This is an application scenario diagram of an intelligent integrated detection system provided in an embodiment of this application; Figure 3 This is a schematic diagram of a differentiated processing flow of a preprocessing unit provided in an embodiment of this application; Figure 4 This is a schematic diagram of the structure of an electronic device provided in an embodiment of this application; Figure 5 This is a schematic flowchart of an integrated multi-media biotoxicity detection method provided in an embodiment of this application; Figure 6 This is a schematic diagram of a process for performing a detection operation provided in an embodiment of this application; Figure 7 This is a schematic diagram of a process for determining a first detection result provided in an embodiment of this application; Figure 8 This is a block diagram of the functional modules of an integrated multi-media biotoxicity detection device provided in this application embodiment. Detailed Implementation

[0013] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present application.

[0014] The terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or apparatus that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products, or apparatuses.

[0015] It should be understood that the term "and / or" in this document is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. Additionally, the character " / " in this document indicates that the preceding and following related objects are in an "or" relationship. In the embodiments of this application, "multiple" refers to two or more.

[0016] In the embodiments of this application, "at least one item" or its similar expression refers to any combination of these items, including any combination of a single item or a plurality of items. "One or more" means one or more, while "multiple" means two or more. For example, "at least one item" of a, b, or c can represent the following seven cases: a, b, c; a and b; a and c; b and c; a, b, and c. Each of a, b, and c can be an element or a set containing one or more elements.

[0017] In this application, the term "connection" refers to various connection methods, such as direct connection or indirect connection, to achieve communication between devices. This application does not impose any limitations on this.

[0018] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0019] In the field of integrated environmental management and risk emergency response, biotoxicity testing of multiple media, including water, soil, air, and solids, is a key means of assessing the degree of harm caused by environmental pollutants. However, existing testing equipment is usually only compatible with a single medium and cannot be adapted to four different forms of media, including water, soil, air, and solids. Furthermore, the pretreatment processes for different media vary significantly, and there is a lack of integrated solutions. This results in low testing efficiency and long testing cycles in multi-media complex pollution scenarios, making it difficult to achieve rapid on-site testing.

[0020] Therefore, improving the detection efficiency of multi-media biotoxicity is an urgent problem to be solved.

[0021] To address the aforementioned issues, this application provides an integrated multi-media biotoxicity detection method and related apparatus. The method involves preprocessing the first sample according to its corresponding sample type to obtain a liquid test sample. The first sample is any one of the collected multi-media samples, including at least one of the following: water sample, gaseous sample, soil sample, and solid waste sample. The liquid test sample and a pre-set liquid control sample are quantitatively added to the sample detection chamber and control detection chamber of a first detection tube, respectively. The first detection tube is an integrated dual-chamber detection tube with independent sample and control detection chambers. Simultaneous bacterial mixing and biochemical reactions are performed on the liquid test sample and the liquid control sample to obtain a first target mixed sample and a second target mixed sample, respectively. Simultaneous detection operations are performed on the first target mixed sample and the second target mixed sample to obtain first luminescence intensity data and second luminescence intensity data, respectively. A first detection result for the first sample is determined based on the first and second luminescence intensity data.

[0022] It is evident that by performing differentiated pretreatment on samples from different media to uniformly convert them into liquid test samples, and using an integrated dual-lumen detection tube to simultaneously perform bacterial mixing, biochemical reactions, and luminescence detection on the liquid test samples and liquid control samples, and then determining the sample test results based on the detection data, the detection efficiency of multi-media biotoxicity is improved.

[0023] For easier understanding, please refer to Figure 1 , Figure 1 This is a system architecture diagram of an intelligent integrated detection system provided in an embodiment of this application. The intelligent integrated detection system includes a control center, a pretreatment unit, an automatic sample addition unit, a detection tube supply unit, a bacterial culture storage unit, a temperature-controlled reaction unit, a dual-channel detection unit, and a cleaning and recovery unit. Each unit works collaboratively under the unified scheduling of the control center to realize an integrated and automated detection process from multi-media sample differential pretreatment, quantitative sample addition, bacterial culture mixing, isothermal reaction, dual-channel synchronous luminescence detection to toxicity level determination and result output.

[0024] The control center is the core scheduling and data processing unit of the system. It is responsible for controlling the collaborative work of each unit and executing the entire detection process. It can calculate the relative luminescence and luminescence inhibition rate based on the first luminescence intensity data and the second luminescence intensity data, determine the toxicity level of the first sample based on the preset threshold, and generate and output the detection results.

[0025] The pretreatment unit is used to perform differentiated pretreatment on the first sample according to its sample type, uniformly converting water samples, gaseous samples, soil samples, or solid waste samples into liquid samples to be tested. The pretreatment unit includes a filtration component for water samples, an air pump and absorption liquid reaction chamber for gaseous samples, a weighing unit, an ultrasonic extraction component, and a filtration component for soil / solid waste samples.

[0026] The automatic sampling unit is used to quantitatively add liquid test samples and liquid control samples to the sample detection chamber and control detection chamber of the first detection tube, respectively, and quantitatively add luminescent bacterial solution to both detection chambers. The automatic sampling unit includes a three-axis robotic arm, a sampling needle, and matching tubing, with a sampling positioning accuracy of ≤0.05mm.

[0027] The test tube supply unit is used to store, push, and replace disposable integrated dual-cavity test tubes. It pushes the new first test tube to the test station and recycles the used test tube to the waste bin after the test is completed.

[0028] The bacterial culture storage unit is used to preserve the luminescent bacterial culture at a constant temperature of 4°C to ensure the activity of the bacterial culture, and to quantitatively output the bacterial culture to the automatic sample dispensing unit according to the instructions of the control center.

[0029] The temperature control reaction unit is used to keep the reaction environment temperature of the first detection tube constant at 25°C after the bacterial solution is mixed, so as to provide stable constant temperature conditions for the biochemical reaction between luminescent bacteria and the sample, and the reaction time is controlled to 15 minutes.

[0030] The dual-channel detection unit is used to simultaneously perform luminescence detection on the sample detection chamber and the control detection chamber of the first detection tube. This dual-channel detection unit includes two independent high-sensitivity photodetectors that respectively acquire the luminescence signals from the sample chamber and the control chamber. After photoelectric conversion, signal amplification and filtering for noise reduction, and analog-to-digital conversion, the first and second luminescence intensity data are obtained.

[0031] The cleaning and recycling unit is used to automatically clean and empty the pipelines and reaction chamber of the pretreatment unit, the sampling needle and pipeline of the automatic sampling unit after a single test, so as to avoid cross-contamination between different batches of samples and to complete the waste recycling in conjunction with the test tube supply unit.

[0032] It is evident that by using differentiated pretreatment to transform water, soil, air, and solid media into liquid samples and enter a standardized testing process, combined with dual-channel synchronous detection and scenario-adaptive toxicity assessment, integrated, rapid, and accurate detection of multi-media biological toxicity is achieved, with a single detection interval of ≤3 minutes, significantly improving detection efficiency and on-site emergency response capabilities in complex pollution scenarios.

[0033] For easier understanding, please refer to Figure 2 , Figure 2 This is an application scenario diagram of an intelligent integrated detection system provided in an embodiment of this application. The first sample is any one of the multi-media samples composed of water samples, gaseous samples, soil samples, and solid waste samples. The first sample is input into the intelligent integrated detection system. The intelligent integrated detection system performs operations such as differentiated pretreatment, automatic sample addition, bacterial solution mixing, isothermal biochemical reaction, dual-channel luminescence acquisition, data calculation, and adaptive threshold determination of toxicity level based on the sampling scenario on the first sample, and outputs the first detection result. The first detection result includes the sample type, relative luminescence, luminescence inhibition rate, toxicity level, and detection time of the first sample, which are not specifically limited here.

[0034] For easier understanding, please refer to Figure 3 , Figure 3 This is a schematic diagram of a differentiated processing flow for a pretreatment unit provided in an embodiment of this application. The pretreatment unit can receive water samples, perform filtration on them using a filtration component to remove suspended particulate matter, and output a liquid sample for testing. The pretreatment unit can also receive gaseous samples, pass them through an absorption liquid at a preset flow rate using an air pump to perform gas-liquid conversion, and output a liquid sample for testing. The pretreatment unit can also receive soil samples or solid waste samples, perform extraction and solid-liquid separation on them, and output a liquid sample for testing. This differentiated process allows the pretreatment unit to uniformly convert multi-media samples of different forms into liquid samples for testing, eliminating media differences and facilitating subsequent unified automated testing processes.

[0035] The following is combined Figure 4 The electronic devices in the embodiments of this application will be described. Figure 4 This is a schematic diagram of the structure of an electronic device provided in an embodiment of this application, such as... Figure 4 As shown, the electronic device includes one or more processors, a memory, a communication interface, and one or more programs. The processor is connected to the memory and the communication interface via an internal communication bus.

[0036] The processor can be used for: The first sample is preprocessed according to the first sample type corresponding to the first sample to obtain a liquid sample to be tested; the first sample is any one of the multi-media samples that have been collected, and the multi-media samples include at least one of the following: water sample, gaseous sample, soil sample, and solid waste sample. The liquid sample to be tested and the preset liquid control sample are quantitatively added to the sample detection chamber and control detection chamber of the first detection tube, respectively; the first detection tube is an integrated dual-chamber detection tube with the sample detection chamber and the control detection chamber being independent of each other; The liquid test sample and the liquid control sample were simultaneously mixed with bacterial solution and subjected to biochemical reaction to obtain the first target mixed sample and the second target mixed sample, respectively. For the first target mixed sample and the second target mixed sample, detection operations are performed simultaneously to obtain the first luminescence intensity data and the second luminescence intensity data, respectively; Based on the first luminescence intensity data and the second luminescence intensity data, the first detection result of the first sample is determined.

[0037] The one or more programs are stored in the aforementioned memory and configured to be executed by the aforementioned processor, and the one or more programs include instructions for performing any step in the above method embodiments.

[0038] The processor can be a central processing unit (CPU), a general-purpose processor, a digital signal processor (DSP), an application-specific integrated circuit (ASIC), a field-programmable gate array (FPGA), or other programmable logic devices, transistor logic devices, hardware components, or any combination thereof. It can implement or execute the various exemplary logic blocks, cells, and circuits described in conjunction with the disclosure of this application. The processor can also be a combination that implements computational functions, such as a combination of one or more microprocessors, a combination of a DSP and a microprocessor, etc. The communication unit can be a communication interface, transceiver, transceiver circuit, etc., and the storage unit can be a memory.

[0039] The memory can be volatile or non-volatile, or a combination of both. Non-volatile memory can be read-only memory (ROM), programmable read-only memory (PROM), erasable programmable read-only memory (EPROM), electrically erasable programmable read-only memory (EEPROM), or flash memory. Volatile memory can be random access memory (RAM), used as an external cache. By way of example, but not limitation, many forms of random access memory (RAM) are available, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate synchronous DRAM (DDR SDRAM), enhanced synchronous DRAM (ESDRAM), synchronous linked DRAM (SLDRAM), and direct rambus RAM (DR RAM).

[0040] It is understood that the electronic device may include more or fewer structural elements than those shown in the block diagram above, such as a power module, physical buttons, a Wi-Fi module, a speaker, a Bluetooth module, sensors, a display module, etc., without limitation. It is understood that the electronic device may incorporate elements such as... Figure 1 The system architecture described above.

[0041] After understanding the software and hardware architecture of this application, the following will be combined with... Figure 5 This application describes an integrated multi-media biotoxicity detection method. Figure 5 This is a flowchart illustrating an integrated multi-media biotoxicity detection method provided in this application embodiment, specifically including the following steps: Step S501: Preprocess the first sample according to the first sample type corresponding to the first sample to obtain the liquid sample to be tested.

[0042] The first sample is any one of the collected multi-media samples, which includes at least one of the following: water sample, gaseous sample, soil sample, and solid waste sample.

[0043] The specific steps of preprocessing the first sample according to the first sample type corresponding to the first sample to obtain the liquid test sample include: S11. If the first sample type of the first sample is the water sample, then the first sample is filtered to obtain the liquid sample to be tested. S12. If the first sample type of the first sample is the gaseous sample, then the first sample is passed into the preset absorption liquid for gas-liquid conversion according to the preset flow rate and preset first duration to obtain the liquid sample to be tested. S13. If the first sample type of the first sample is the soil sample or the solid waste sample, then the first sample is subjected to leaching treatment and solid-liquid separation to obtain the liquid sample to be tested.

[0044] In a specific embodiment, when the first sample is a water sample, the collected water sample is directly passed into the filtration assembly. This filtration assembly contains a microporous membrane with a pore size ranging from 0.45 μm to 1.0 μm, used to remove suspended particulate matter, silt, algae, and other interfering substances from the water. The filtered clear liquid water sample is the liquid sample to be tested, and is transported through pipelines to the sample dispensing position of the automatic sampling unit.

[0045] When the first sample is a gaseous sample, the gas pump is activated, and the gaseous sample is introduced into the reaction chamber containing a special absorbent liquid at a preset flow rate (e.g., 0.5 L / min). This special absorbent liquid can be selected according to the target analyte, such as deionized water or a specific buffer solution, to fully dissolve volatile organic compounds, acidic gases, and other toxic substances in the gaseous sample. The gas is continuously introduced for a preset first duration (e.g., 30 minutes) to complete the gas-liquid conversion. After the absorbent liquid in the reaction chamber absorbs the toxic substances, it becomes a liquid sample to be tested, which is then transported to the sample dispensing position of the automatic sample dispensing unit.

[0046] If the first sample type is a soil sample or a solid waste sample, pure water is added to the first sample according to the preset solid-liquid ratio, and ultrasonic-assisted extraction is used to fully release the toxic substances in the first sample into the liquid. Then, the extraction mixture is subjected to solid-liquid separation, and the liquid sample to be tested is obtained after removing the solid residue.

[0047] It is evident that by performing differentiated preprocessing for different sample types, water, soil, air, and solid multi-media samples are uniformly transformed into liquid test samples, eliminating the differences in media morphology and laying the foundation for subsequent unified and standardized biotoxicity testing.

[0048] The specific steps of extracting and separating the first sample to obtain the liquid sample to be tested include: S21. Obtain the sample weight corresponding to the first sample; S22. Determine the weight of pure water based on the sample weight and the preset weight ratio; S23. Inject the pure water corresponding to the weight of the pure water into the reaction chamber of the first sample, and perform extraction treatment for a preset second time to obtain a reference mixture. S24. Perform solid-liquid separation on the reference mixture to obtain the liquid sample to be tested.

[0049] In a specific embodiment, firstly, the collected first sample (such as a soil sample or a solid waste sample) is placed on the weighing unit of the pretreatment unit, and the sample weight is obtained using a high-precision electronic balance. This sample weight is automatically transmitted to the system's control center and recorded, serving as a baseline parameter for subsequent pure water ratio calculations. For example, a surface soil sample from a contaminated site is weighed, and the measured sample weight is 100g. Then, the control center calculates the required weight of pure water based on the measured sample weight and a preset weight ratio (such as 1:4). Pure water weight = sample weight × 4. For example, if the sample weight is 100g, the pure water weight is 400g (approximately 400mL). The 1:4 solid-liquid ratio ensures complete immersion of the first sample and sufficient dissolution of toxic substances, while avoiding excessive dilution that could lead to levels below the detection limit.

[0050] Next, pure water corresponding to the weight of the first sample is injected into the reaction chamber, and the ultrasonic stirring component is activated. The ultrasonic frequency can be set to 40kHz. Through the cavitation effect and mechanical stirring of the ultrasound, the sample particles are dispersed, promoting the mass transfer and dissolution of toxic substances such as heavy metal ions and organic pollutants in the sample into the aqueous phase. The extraction process is continued for a preset second duration (e.g., 20 minutes) to obtain a reference mixture of the first sample and pure water. After extraction, the reference mixture is passed through a filtration component, which can use a microporous membrane filter with a pore size of 0.45μm. Through filtration, solid residues such as silt and particulate matter are separated from the liquid phase containing toxic substances, resulting in a liquid sample of soil / solid waste to be tested, i.e., the liquid sample to be tested. Then, it is transported through pipeline to the sample loading position of the automatic sample loading unit, awaiting subsequent sample loading operations.

[0051] It is evident that the process of weighing and measuring, injecting pure water according to a preset solid-liquid ratio, ultrasonic immersion and solid-liquid separation ensures that toxic substances in soil / solid waste samples are fully dissolved and that interference from solid residues is effectively removed, providing standardized liquid test samples for subsequent testing.

[0052] Step S502: The liquid sample to be tested and the preset liquid control sample are quantitatively added to the sample detection chamber and control detection chamber of the first detection tube, respectively.

[0053] The first detection tube is an integrated dual-cavity detection tube, which has a sample detection cavity and a control detection cavity that are independent of each other.

[0054] Specifically, firstly, the first detection tube is pushed from the storage compartment to the detection station and fixed by the detection tube supply unit. The first detection tube is a disposable integrated dual-chamber detection tube, with independent and physically isolated sample detection chamber and control detection chamber inside. The two chambers are symmetrical in structure and made of the same material to ensure that the subsequent optical detection conditions are the same. Then, the automatic sample addition unit is activated, and the three-axis robotic arm drives the sample addition needle to accurately position it to the sample addition location with a positioning accuracy of ≤0.05mm. The sample addition process is executed synchronously in two groups: the first sample addition channel draws a preset volume (e.g., 10mL) of liquid sample to be tested from the sample position and injects it into the sample detection chamber; the second sample addition channel draws an equal volume (10mL) of liquid control sample (e.g., 3% sodium chloride matrix balanced solution) from the control solution storage bottle and injects it into the control detection chamber. The two sample addition actions are controlled collaboratively by the same three-axis robotic arm to ensure consistent sample volume and synchronized actions. After the sample addition is completed, the sample addition needle can be cleaned by the cleaning component to avoid cross-contamination between different samples. In the first detection tube, both the sample detection chamber and the control detection chamber are filled with the corresponding liquids, ready to proceed to the next stage of bacterial solution mixing.

[0055] In one possible embodiment, the first detection tube after sample addition can be transferred to a pre-set integrated detection chamber for subsequent biochemical reactions and detection. The integrated detection chamber is a 20cm × 20cm square chamber, comprising a digestion zone, a heat insulation layer, and a detection zone. The digestion zone consists of a total phosphorus digestion zone, a spacer section, and a total nitrogen digestion zone. Each of the total phosphorus and total nitrogen digestion zones has a digestion tube port for placing the digestion tube, completing the high-temperature digestion pretreatment of the water sample for total phosphorus and total nitrogen. A heat insulation layer is installed between the digestion zone and the detection zone. This layer isolates the heat generated during digestion, preventing downward conduction of high temperatures and avoiding disruption of the constant temperature environment in the detection zone or interference with optical detection results.

[0056] The detection area includes one shared well for physicochemical detection tubes, which is used by the ammonia nitrogen detector, chemical oxygen demand (COD) detector, total nitrogen detector, and total phosphorus detector. A three-axis robotic arm sequentially places and removes reaction tubes after color development into this well, performing time-sharing detection of four physicochemical indicators: ammonia nitrogen, COD, total nitrogen, and total phosphorus. The detection area also includes four independent wells for total toxicity detection tubes, each equipped with its own independent total toxicity detector. The first detection tube, after sample addition, is transferred and positioned into the total toxicity detection well by the three-axis robotic arm. An integrated detection chamber provides a stable, light-proof, and temperature-controlled internal environment for subsequent bacterial mixing, biochemical reactions, and luminescence signal acquisition.

[0057] It should be noted that the four total toxicity detection tube positions can accommodate two tube arrangement methods: When using the first detection tube (i.e., a disposable integrated dual-lumen detection tube), a single total toxicity detection tube position can accommodate one first detection tube integrating the sample detection chamber and the control detection chamber, and four sets of total toxicity comparison experiments can be performed in parallel across the four positions; when using separate sample and control detection tubes, one set of total toxicity comparison experiments occupies two total toxicity detection tube positions, with the sample detection tube and control detection tube placed separately, and a maximum of two sets of total toxicity comparison experiments can be performed simultaneously across the four positions. Each total toxicity detection tube position is independent of the others, and each position can independently place the reaction tube, independently conduct the biochemical incubation reaction, and independently complete the optical signal acquisition.

[0058] In one possible embodiment, the required range for ammonia nitrogen detection is 5-100 mg / L, with a required accuracy of 1 mg / L. The actual detection range of the instrument is 0.5-10 mg / L. High-concentration water samples need to be diluted 10-fold, resulting in an actual detection accuracy of 0.5 mg / L. The ammonia nitrogen detection is performed as follows: First, the flow path is switched between a valve and a peristaltic pump, allowing selection of either pure water or the river water sample to be tested. 3 mL of the river water sample is drawn using a syringe and injected into a colorimetric tube pre-filled with colorimetric reagent, placed in a colorimetric tube holder. A three-axis robotic arm shakes the colorimetric tube to fully dissolve the reagent in the water sample, allowing it to stand at room temperature for 20 minutes for a complete colorimetric reaction. After the reaction, the three-axis robotic arm shakes the colorimetric tube again, moves and transfers it to the shared physicochemical detection tube aperture in the integrated detection chamber, and places it in position. The internal sensor of the detector is activated to perform optical detection, acquire optical signals, and analyze the data to obtain the ammonia nitrogen detection result.

[0059] In one possible embodiment, the COD detection index is required to be in the range of 5-100 mg / L, with a required accuracy of 1 mg / L. The actual detection range of the instrument is 0.5-10 mg / L. High-concentration water samples need to be diluted 10-100 times, and the actual detection accuracy is 5 mg / L. The COD detection is performed according to the following steps: First, the flow path is switched between a valve and a peristaltic pump, and either pure water or the river water sample to be tested can be selected. 3 mL of the river water sample to be tested is extracted using a sampling needle and injected into a colorimetric tube placed in a colorimetric tube holder, which is pre-filled with colorimetric reagent. A three-axis robotic arm shakes the colorimetric tube to fully dissolve the colorimetric reagent in the water sample. The sample is allowed to stand at room temperature for 10 minutes to allow for sufficient colorimetric reaction. After the reaction is completed, the three-axis robotic arm shakes the colorimetric tube again and moves and transfers the colorimetric tube to the common physicochemical detection tube position in the detection area of ​​the integrated detection chamber and places it in place. The internal sensor of the detector is activated to perform optical detection, collect optical signals, and analyze the data to obtain the COD detection result.

[0060] In one possible embodiment, the total nitrogen detection target range is 10-50 mg / L, with a required accuracy of 1 mg / L. The actual instrument detection range is 0.5-10 mg / L. High-concentration water samples require a 10-fold dilution, resulting in an actual detection accuracy of 0.5 mg / L. The total nitrogen detection is performed as follows: The digestion preheating module is started 15 minutes in advance. The flow path is then switched via a valve and peristaltic pump, allowing selection of either pure water or the river water sample to be tested. A 10 mL sample of the river water to be tested is taken using a syringe and added to a digestion tube containing the digestion reagent. After shaking and allowing to stand, the digestion reaction is carried out using ultraviolet light combined with 60°C heating for 18 minutes. After digestion, 0.9 mL of sample is taken from the digestion tube and placed in the first reaction zone of the colorimetric tube, which is isolated by a plastic film baffle and pre-filled with reagent A (such as a pH-adjusting buffer to neutralize the strongly alkaline system of the digested sample). The mixture is then shaken using a three-axis robotic arm and reacted for 30 seconds. Next, the first layer of plastic film baffle is pierced by the injection needle, and the reaction solution enters the second reaction zone of the colorimetric tube under gravity. It is mixed with 2.7 mL of reagent B (such as acidic medium and interference masking reagent, which provides an acidic environment for optical detection and masks metal ion interference) in the second reaction zone and shaken well. The reaction continues for 10 minutes. After the reaction is completed, the three-axis robotic arm moves and transports the colorimetric tube to the common physicochemical detection tube hole in the detection zone of the integrated detection chamber and places it in place. The internal sensor of the detector is activated to carry out optical detection, collect optical signals and analyze data to obtain the total nitrogen detection result.

[0061] In one possible embodiment, the total phosphorus detection target range is 5-15 mg / L, with a required accuracy of 1 mg / L. The actual instrument detection range is 0.2-2 mg / L. For high-concentration water samples, a 10-fold dilution can be performed, resulting in an actual detection accuracy of 0.5 mg / L. The total phosphorus detection is performed as follows: the flow path is switched between a valve and a peristaltic pump. Pure water or the river water sample to be tested can be selected. A 10 mL sample of the river water to be tested is taken using a sampling needle and added to a digestion tube containing digestion reagent. After shaking well, the sample is allowed to stand. The digestion reaction is carried out using ultraviolet light combined with heating at 60°C for 15 minutes. After digestion, 3 mL of sample is taken from the digestion tube and placed into the first reaction zone of the colorimetric tube on the colorimetric tube holder. This first reaction zone is isolated by a plastic film baffle and is pre-filled with reagent A. The sample is then shaken using a triaxial robotic arm and reacted for 1 minute. Next, the first plastic film baffle is punctured by the injection needle, and the reaction solution enters the second reaction zone of the colorimetric tube under gravity. It is then mixed with the colorimetric reagent in the second reaction zone and shaken, and the reaction continues for 5 minutes. After the reaction is complete, the triaxial robotic arm moves and transports the colorimetric tube to the shared physicochemical detection tube port in the detection area of ​​the integrated detection chamber and places it in place. The internal sensor of the detector is activated to perform optical detection, collect optical signals, and analyze the data to obtain the total phosphorus detection result.

[0062] In one possible embodiment, the sample to be tested can be river water, soil extract, or gas collection liquid. The total biotoxicity test is performed according to the following steps: First, sampling is completed using a syringe needle. 2 mL of the river water sample to be tested is added to the sample group's luminescent tube, while the control group's luminescent tube contains 2 mL of pre-contained sodium chloride (NaCl) solution. 1 mL of bacterial solution, stored at 4°C, is added to both the sample group's and control group's luminescent tubes. After shaking each system thoroughly, the reaction is carried out for 15 minutes. After the reaction is complete, the luminescent tubes of the sample group and control group are placed into the detector's detection well. The sensor is activated to perform detection, acquiring detection signals and analyzing data to complete the total biotoxicity test. The detector's detection well can be shared with or independently set with the physicochemical indicators, acquiring detection data for the sample group and control group respectively. The total biotoxicity result of the sample is obtained by comparing the two sets of data.

[0063] It is evident that by sharing a three-axis robotic arm and an integrated detection chamber, the physicochemical concentrations of ammonia nitrogen, COD, total nitrogen, and total phosphorus, as well as the biological effects of total biological toxicity, can be detected in a time-sharing manner. By combining quantitative detection of physicochemical pollutant concentrations with bioluminescent bacteria-based total biological toxicity detection, it is possible to evaluate the degree of organic pollution and eutrophication in water bodies, and to characterize the comprehensive ecotoxicological risks brought about by the synergistic effects of multiple pollutants. This enables multi-dimensional comprehensive water quality evaluation and is adaptable to various test samples such as river water, soil leachate, and gas collection liquid, achieving integrated automatic detection.

[0064] Step S503: Simultaneously perform bacterial mixing and biochemical reaction on the liquid test sample and the liquid control sample to obtain the first target mixed sample and the second target mixed sample, respectively.

[0065] The steps of simultaneously mixing bacterial solutions and performing biochemical reactions on the liquid test sample and the liquid control sample to obtain a first target mixed sample and a second target mixed sample include: S31. Obtain the sample volume of the liquid sample to be tested; S32. Determine the bacterial culture volume based on the sample volume and the preset volume ratio; S33. The luminescent bacterial solution corresponding to the volume of the bacterial solution is uniformly mixed with the liquid test sample and the liquid control sample to obtain the first reference mixed sample and the second reference mixed sample. S34. According to the preset ambient temperature and reaction time, control the first reference mixed sample and the second reference mixed sample to carry out biochemical reaction to obtain the first target mixed sample and the second target mixed sample respectively.

[0066] In a specific embodiment, firstly, the system's control center reads the volume of the liquid sample to be tested, already injected into the sample detection chamber, from the automatic sampling unit. Then, based on the sample volume and a preset sample-to-bacterial-liquid volume ratio (e.g., 5:1), the required volume of luminescent bacterial solution to be added (e.g., the first bacterial solution volume) is calculated. The first bacterial solution volume = sample volume ÷ 5. For example, if the sample volume is 10 mL, then the first bacterial solution volume is 2 mL. Using a 5:1 ratio ensures that the luminescent bacteria reach a suitable concentration in the mixed solution, guaranteeing both the intensity of the luminescence signal and preventing the sensitivity of the toxicity response from being affected by excessive or insufficient bacterial solution.

[0067] Then, the peristaltic pump of the bacterial solution storage unit quantitatively outputs the luminescent bacterial solution stored in a constant temperature environment of 4°C. When using the first detection tube, the same volume (i.e., the volume of the first bacterial solution) of luminescent bacterial solution can be injected into both the sample detection chamber and the control detection chamber of the first detection tube through the tubing. After injection, the triaxial robotic arm shakes the sample detection chamber and the control detection chamber to mix the bacterial solution with the sample and sodium chloride evenly. After mixing in both detection chambers, the first reference mixed sample in the sample detection chamber and the second reference mixed sample in the control detection chamber are obtained, respectively. When using separate sample and control detection tubes, the same volume of luminescent bacterial solution can be injected into both the sample and control detection tubes through the tubing. After injection, the triaxial robotic arm shakes the sample and control detection tubes to mix the bacterial solution with the sample and sodium chloride evenly. After mixing in both detection tubes, the first reference mixed sample in the sample detection tube and the second reference mixed sample in the control detection tube are obtained, respectively.

[0068] Next, the temperature-controlled reaction unit is activated to precisely control the temperature of the reaction area where the first detection tube is located. Through closed-loop control of the heating element and temperature sensor, the ambient temperature is maintained constant at 25℃, with temperature fluctuations controlled within ±0.5℃. 25℃ is the optimal temperature for the metabolic activity of luminescent bacteria; at this temperature, toxic substances fully interact with the luminescent bacteria, ensuring the stability and sensitivity of the toxic response. The first and second reference mixed samples undergo simultaneous biochemical reactions under constant temperature of 25℃, with the reaction time precisely controlled by the system timer to be 15 minutes. During the biochemical reaction, the first detection tube remains in a light-protected state to avoid interference from external light on the luminescent bacteria. After the reaction, the first target mixed sample in the sample detection chamber and the second target mixed sample in the control detection chamber are obtained, respectively, and proceed to the next stage of photometric detection.

[0069] It is evident that by quantitatively adding bacterial solution and achieving uniform mixing through bubbling, and controlling the reaction time under constant temperature conditions, the biochemical reaction between luminescent bacteria and the sample can be ensured to be sufficient and stable, providing a reliable sample reaction system for dual-channel synchronous detection.

[0070] Step S504: Simultaneously perform detection operations on the first target mixed sample and the second target mixed sample to obtain the first luminescence intensity data and the second luminescence intensity data, respectively.

[0071] For easier understanding, please refer to Figure 6 , Figure 6 This is a flowchart illustrating a detection operation provided in an embodiment of this application. The step of simultaneously performing detection operations on the first target mixed sample and the second target mixed sample to obtain first luminescence intensity data and second luminescence intensity data, specifically includes: S41. Collect the emission signals of the first target mixed sample and the second target mixed sample respectively to obtain the first emission signal and the second emission signal; S42. Convert the first light-emitting signal and the second light-emitting signal into electrical signals respectively to obtain a first reference electrical signal and a second reference electrical signal; S43. The first reference electrical signal and the second reference electrical signal are respectively subjected to noise reduction and amplification processing to obtain the first target electrical signal and the second target electrical signal; S44. Perform analog-to-digital conversion on the first target electrical signal and the second target electrical signal respectively to obtain the first luminous intensity data and the second luminous intensity data.

[0072] In a specific embodiment, the dual-channel detection unit is activated. This dual-channel detection unit comprises two independent and symmetrically arranged high-sensitivity photodetectors, respectively aligned with the sample detection chamber and the control detection chamber of the first detection tube. The entire detection process is conducted within a light-proof chamber to prevent interference from ambient light. The two photodetectors operate synchronously: the first-channel detector collects the biofluorescence emitted by the first target mixed sample in the sample detection chamber, obtaining a first luminescence signal; the second-channel detector simultaneously collects the biofluorescence emitted by the second target mixed sample in the control detection chamber, obtaining a second luminescence signal. The acquisition time and integration time of the two sets of signals are completely consistent, ensuring the temporal synchronization and comparability of the data.

[0073] Then, the photoelectric conversion element inside the photodetector (such as a photomultiplier tube or silicon photodiode) converts the collected light signal into a corresponding electrical signal. The higher the luminous intensity, the greater the photocurrent generated, and the higher the amplitude of the electrical signal. After photoelectric conversion, the first luminous signal is converted into a first reference electrical signal, and the second luminous signal is converted into a second reference electrical signal. At this point, the electrical signal is a weak analog signal, with an amplitude typically in the nanoampere to microampere range.

[0074] Next, the first and second reference electrical signals are input into their respective signal processing circuits. These signal processing circuits include signal amplification and filtering circuits. The signal amplification circuit amplifies the weak electrical signal to an amplitude range suitable for subsequent processing (e.g., millivolts to volts). The signal filtering circuit uses low-pass or band-pass filters to filter out high-frequency electronic noise, power frequency interference, and other non-signal components, improving the signal-to-noise ratio. After noise reduction and amplification, the first target electrical signal and the second target electrical signal are obtained, respectively.

[0075] Finally, the first and second target electrical signals are respectively sent to an analog-to-digital converter (ADC) to convert the analog voltage signals into digital signals. The ADC has a resolution of at least 16 bits to ensure that signal details are fully preserved. After ADC conversion, first and second luminous intensity data are obtained, both of which are digital quantities that can be directly calculated by the data processing unit. These two sets of data are transmitted to the system's control center via a data bus, serving as the direct basis for calculating the luminescence inhibition rate and determining the toxicity level.

[0076] It is evident that by synchronously acquiring luminescence signals through dual channels, and processing them through photoelectric conversion, noise reduction amplification, and analog-to-digital conversion, environmental interference and electronic noise can be effectively eliminated, ensuring the synchronicity, accuracy, and reliability of luminescence intensity data between the sample group and the control group, thus providing a high-quality data foundation for the accurate determination of toxicity levels.

[0077] Step S505: Determine the first detection result of the first sample based on the first luminescence intensity data and the second luminescence intensity data.

[0078] For easier understanding, please refer to Figure 7 , Figure 7 This is a flowchart illustrating a method for determining a first detection result according to an embodiment of this application. The specific steps for determining the first detection result of the first sample based on the first luminescence intensity data and the second luminescence intensity data include: S51. Determine the first relative luminescence intensity based on the first luminescence intensity data and the second luminescence intensity data; S52. Determine the first luminescence suppression rate based on the first relative luminescence intensity; S53. Determine the first toxicity level of the first sample based on the first luminescence inhibition rate; S54. Determine the first detection result based on the first sample type, the first relative luminescence, the first luminescence inhibition rate, and the first toxicity level.

[0079] In a specific embodiment, the control center first calls a preset relative luminescence calculation formula, substituting the first and second luminescence intensity data into the calculation. The formula for calculating relative luminescence is: First relative luminescence = (First luminescence intensity data / Second luminescence intensity data) × 100%. Here, the first luminescence intensity data is the luminescence intensity corresponding to the sample detection chamber, reflecting the luminescent bacterial activity of the test sample; the second luminescence intensity data is the luminescence intensity corresponding to the control detection chamber, reflecting the luminescent bacterial activity of the blank control group unaffected by toxic substances. By calculating the ratio, the influence of batch differences in bacterial solutions and minor fluctuations in ambient temperature on the absolute value of luminescence intensity can be eliminated, making the test results comparable. For example, if the first luminescence intensity data is 4200 (relative units) and the second luminescence intensity data is 10000 (relative units), then the first relative luminescence is 42%.

[0080] Then, the control center calculates the first luminescence inhibition rate based on the first relative luminescence intensity. This first luminescence inhibition rate reflects the degree to which the toxic substance in the sample inhibits the luminescence intensity of the luminescent bacteria. The calculation formula is: First luminescence inhibition rate = 100% - First relative luminescence intensity. Wherein, if the first relative luminescence intensity is 42%, then the first luminescence inhibition rate is 100% - 42% = 58%. The higher the first luminescence inhibition rate, the stronger the overall toxicity of the toxic substance in the first sample. Next, the first toxicity level is determined based on the comparison result of the first luminescence inhibition rate and a preset threshold. Finally, the first sample type, first relative luminescence intensity, first luminescence inhibition rate, and first toxicity level are integrated to obtain the first detection result.

[0081] The entire data processing and judgment process can be completed within 3 minutes to meet the timeliness requirements of rapid on-site detection and emergency early warning. After the detection is completed, the system automatically executes a reset procedure: the detection tube supply unit recovers the first used detection tube to the waste bin; the cleaning and recycling unit automatically cleans and empties the pipelines of the pretreatment unit, the reaction chamber, and the sampling needle of the automatic sampling unit; all mechanical structures return to their initial state. The system re-enters the testing state and can perform cyclical testing on the next batch of multi-media samples.

[0082] As can be seen, by sequentially calculating the relative luminescence and luminescence inhibition rate and classifying the toxicity, and integrating multi-dimensional data to generate complete test results, the test results data are guaranteed to be complete and the traceability is clear.

[0083] The specific steps for determining the first toxicity level of the first sample based on the first luminescence inhibition rate include: S61. Obtain the sampling scenario information corresponding to the multi-media sample; the sampling scenario information includes at least one of the following: emergency detection scenario, key detection scenario, and routine detection scenario; S62. Determine the threshold adjustment coefficient based on the sampling scenario information; S63. Obtain a preset first reference threshold and a second reference threshold; the second reference threshold is greater than the first reference threshold; S64. Adjust the first reference threshold and the second reference threshold according to the threshold adjustment coefficient to obtain the first target threshold and the second target threshold; S65. If the first luminescence inhibition rate is less than or equal to the first target threshold, then the first toxicity level of the first sample is determined to be low toxicity. S66. If the first emission inhibition rate is greater than the first target threshold and less than the second target threshold, then the first toxicity level of the first sample is determined to be moderate toxicity. S67. If the first luminescence inhibition rate is greater than or equal to the second target threshold, then the first toxicity level of the first sample is determined to be high toxicity.

[0084] In a specific embodiment, sampling scene information corresponding to the current detection task is obtained. This sampling scene information is selected by the operator before detection via a drop-down menu or shortcut key, or it can be automatically determined by the system based on GPS positioning coordinates and a preset area database. The sampling scene information includes the following three types: Emergency detection scenarios: Applicable to emergencies such as sudden environmental pollution accidents and abnormal water quality in drinking water sources, requiring the fastest possible screening of potential toxic risks, with no omissions allowed; Key testing scenarios: Applicable to regulatory tasks such as investigation of contaminated sites and special inspections of industrial sewage outlets. It is necessary to make judgments more stringent than the conventional standards to reduce the risk of missing potential pollution sources. Routine testing scenarios: Suitable for routine monitoring tasks such as watershed water environment monitoring and soil surveys, using default standard thresholds to balance testing efficiency and result reliability.

[0085] Then, the system can call the internally stored mapping table between sampling scenarios and threshold adjustment coefficients, and automatically determine the corresponding threshold adjustment coefficient based on the acquired sampling scenario information. Specifically, the threshold adjustment coefficient for emergency detection scenarios in the mapping table is 0.8 to minimize the risk of missed detections; the threshold adjustment coefficient for key detection scenarios is 0.9; and the threshold adjustment coefficient for routine detection scenarios is 1.0 to maintain a standard judgment scale.

[0086] Next, the control center reads the preset first and second reference thresholds from the system storage area. Both thresholds are factory default values; the first threshold defaults to 30%, and the second to 70%, without further specific limitations. The second reference threshold is always greater than the first, ensuring that each toxicity level range is mutually exclusive and complete, covering all possible values ​​of the luminescence inhibition rate from 0% to 100%. Then, the first and second reference thresholds are adjusted according to a threshold adjustment coefficient to obtain the first and second target thresholds. The first target threshold = first reference threshold × threshold adjustment coefficient; the second target threshold = second reference threshold × threshold adjustment coefficient.

[0087] Specifically, if the first luminescence inhibition rate is less than or equal to the first target threshold, it indicates that the combined toxic substances in the sample have a weak inhibitory effect on the luminescence of the luminescent bacteria and have not reached the level of moderate toxicity, and the first toxicity level of the first sample is determined to be low toxicity; if the first luminescence inhibition rate is greater than the first target threshold and less than the second target threshold, it indicates that the sample has obvious toxicity and the degree of luminescence inhibition is at an intermediate level, and the first toxicity level is determined to be moderate toxicity; if the first luminescence inhibition rate is greater than or equal to the second target threshold, it indicates that the combined toxic substances in the sample have a strong inhibitory effect on the luminescent bacteria and the luminescence intensity is significantly inhibited, and the first toxicity level is determined to be high toxicity.

[0088] It is evident that by matching the corresponding threshold adjustment coefficient based on the sampling scenario and dynamically correcting the grading threshold, the adaptive and accurate classification of toxicity levels in multiple scenarios can be achieved, taking into account the differentiated use needs of strict emergency warning, precise control of key monitoring and general judgment of routine testing.

[0089] The above primarily describes the solutions of the embodiments of this application from the perspective of the method execution process. It is understood that, in order to achieve the above functions, the electronic device includes corresponding hardware structures and / or software modules for executing each function. Those skilled in the art should readily recognize that, in conjunction with the units and algorithm steps of the various examples described in the embodiments provided herein, this application can be implemented in hardware or a combination of hardware and computer software. Whether a function is executed by hardware or by computer software driving hardware depends on the specific application and design constraints of the technical solution. Those skilled in the art can use different methods to implement the described functions for each specific application, but such implementation should not be considered beyond the scope of this application.

[0090] This application embodiment can divide the electronic device into functional units according to the above method example. For example, each function can be divided into a separate functional unit, or two or more functions can be integrated into one processing unit. The integrated unit can be implemented in hardware or as a software functional unit. It should be noted that the unit division in this application embodiment is illustrative and only represents one logical functional division. In actual implementation, there may be other division methods.

[0091] When dividing each function into modules according to its corresponding function. Figure 8 This is a functional block diagram of a multi-media integrated biotoxicity detection device 800 provided in an embodiment of this application. The multi-media integrated biotoxicity detection device 800 includes a pretreatment module 810, a sample addition module 820, a mixing module 830, a detection module 840, and a determination module 850, wherein: The preprocessing module 810 is used to preprocess the first sample according to the first sample type corresponding to the first sample to obtain a liquid sample to be tested; the first sample is any one of the collected multi-media samples, and the multi-media samples include at least one of the following: water sample, gaseous sample, soil sample, and solid waste sample. The sample addition module 820 is used to quantitatively add the liquid sample to be tested and the preset liquid control sample to the sample detection chamber and the control detection chamber of the first detection tube, respectively; the first detection tube is an integrated dual-chamber detection tube with the sample detection chamber and the control detection chamber being independent of each other; The mixing module 830 is used to simultaneously perform bacterial liquid mixing and biochemical reaction on the liquid test sample and the liquid control sample to obtain a first target mixed sample and a second target mixed sample, respectively. The detection module 840 is used to simultaneously perform detection operations on the first target mixed sample and the second target mixed sample to obtain first luminescence intensity data and second luminescence intensity data, respectively. The determining module 850 is used to determine the first detection result of the first sample based on the first luminous intensity data and the second luminous intensity data.

[0092] Optionally, in the step of preprocessing the first sample according to the first sample type corresponding to the first sample to obtain a liquid sample to be tested, the preprocessing module 810 is specifically used for: If the first sample type of the first sample is the water sample, then the first sample is filtered to obtain the liquid sample to be tested; If the first sample type of the first sample is the gaseous sample, then the first sample is passed into the preset absorption liquid for gas-liquid conversion according to the preset flow rate and preset first duration to obtain the liquid sample to be tested; If the first sample type of the first sample is the soil sample or the solid waste sample, then the first sample is subjected to leaching and solid-liquid separation to obtain the liquid sample to be tested.

[0093] Optionally, in the process of extracting and separating the first sample to obtain the liquid sample to be tested, the preprocessing module 810 is further specifically used for: Obtain the sample weight corresponding to the first sample; The weight of pure water is determined based on the sample weight and the preset weight ratio; The pure water corresponding to the weight of the pure water is injected into the reaction chamber of the first sample, and the extraction process is carried out for a preset second time to obtain a reference mixture. The reference mixture is subjected to solid-liquid separation to obtain the liquid sample to be tested.

[0094] Optionally, in the process of simultaneously mixing bacterial solutions and performing biochemical reactions on the liquid test sample and the liquid control sample to obtain a first target mixed sample and a second target mixed sample, the mixing module 830 is specifically used for: Obtain the sample volume of the liquid sample to be tested; The bacterial culture volume is determined based on the sample volume and the preset volume ratio; The luminescent bacterial solution corresponding to the volume of the bacterial solution is uniformly mixed with the liquid test sample and the liquid control sample to obtain the first reference mixed sample and the second reference mixed sample. According to the preset ambient temperature and reaction time, the first reference mixed sample and the second reference mixed sample are controlled to carry out biochemical reactions to obtain the first target mixed sample and the second target mixed sample, respectively.

[0095] Optionally, in the process of simultaneously performing detection operations on the first target mixed sample and the second target mixed sample to obtain first luminescence intensity data and second luminescence intensity data respectively, the detection module 840 is specifically used for: The emission signals of the first target mixed sample and the second target mixed sample are collected respectively to obtain the first emission signal and the second emission signal; The first light-emitting signal and the second light-emitting signal are converted into electrical signals respectively to obtain a first reference electrical signal and a second reference electrical signal; The first reference electrical signal and the second reference electrical signal are respectively subjected to noise reduction and amplification processing to obtain the first target electrical signal and the second target electrical signal; The first target electrical signal and the second target electrical signal are converted from analog to digital to obtain the first luminous intensity data and the second luminous intensity data, respectively.

[0096] Optionally, in determining the first detection result of the first sample based on the first luminescence intensity data and the second luminescence intensity data, the determining module 850 is specifically used for: Based on the first luminous intensity data and the second luminous intensity data, a first relative luminosity is determined; The first luminescence suppression rate is determined based on the first relative luminescence intensity; The first toxicity level of the first sample is determined based on the first luminescence inhibition rate; The first detection result is determined based on the first sample type, the first relative luminescence, the first luminescence inhibition rate, and the first toxicity level.

[0097] Optionally, in determining the first toxicity level of the first sample based on the first luminescence inhibition rate, the determining module 850 is further specifically configured to: Obtain the sampling scenario information corresponding to the multi-media sample; the sampling scenario information includes at least one of the following: emergency detection scenario, key detection scenario, and routine detection scenario; Determine the threshold adjustment coefficient based on the sampling scenario information; Obtain a preset first reference threshold and a second reference threshold; the second reference threshold is greater than the first reference threshold. The first reference threshold and the second reference threshold are adjusted according to the threshold adjustment coefficient to obtain the first target threshold and the second target threshold; If the first emission inhibition rate is less than or equal to the first target threshold, then the first toxicity level of the first sample is determined to be low toxicity. If the first emission inhibition rate is greater than the first target threshold and less than the second target threshold, then the first toxicity level of the first sample is determined to be moderate toxicity. If the first emission inhibition rate is greater than or equal to the second target threshold, then the first toxicity level of the first sample is determined to be high toxicity.

[0098] It is evident that by performing differentiated pretreatment on samples from different media to uniformly convert them into liquid test samples, and using an integrated dual-lumen detection tube to simultaneously perform bacterial mixing, biochemical reactions, and luminescence detection on the liquid test samples and liquid control samples, and then determining the sample test results based on the detection data, the detection efficiency of multi-media biotoxicity is improved.

[0099] It should be noted that the specific implementation of each operation can be described in the corresponding description of the method embodiments shown above. The multi-media biotoxicity integrated detection device 800 can be used to execute the method embodiments of this application, and will not be described again here.

[0100] This application also provides a computer-readable storage medium storing a computer program for electronic data interchange, which causes a computer to perform some or all of the steps of any of the methods described in the above method embodiments, wherein the computer includes an electronic device.

[0101] This application also provides a computer program product, which includes a non-transitory computer-readable storage medium storing a computer program operable to cause a computer to perform some or all of the steps of any of the methods described in the above method embodiments. The computer program product may be a software installation package, and the computer may include an electronic device.

[0102] It should be noted that, for the sake of simplicity, the above embodiments are all described as a series of actions. Those skilled in the art should understand that this application is not limited to the described order of actions, as some steps in the embodiments of this application can be performed in other orders or simultaneously. Furthermore, those skilled in the art should also understand that the embodiments described in the specification are preferred embodiments, and the actions, steps, modules, or units involved are not necessarily essential to the embodiments of this application.

[0103] In the above embodiments, the descriptions of each embodiment in this application have different focuses. For parts not described in detail in a certain embodiment, please refer to the relevant descriptions in other embodiments.

[0104] Those skilled in the art will understand that all or part of the processes in the methods of the above embodiments can be implemented by a computer program instructing related hardware. This program can be stored in a computer-readable storage medium, and when executed, it can include the processes described in the above method embodiments. The aforementioned storage medium includes various media capable of storing program code, such as ROM or random access memory (RAM), magnetic disks, or optical disks.

[0105] The steps of the methods or algorithms described in the embodiments of this application can be implemented in hardware or by a processor executing software instructions. The software instructions can consist of corresponding software modules, which can be stored in RAM, flash memory, ROM, EPROM, electrically erasable programmable read-only memory (EEPROM), registers, hard disk, portable hard disk, read-only optical disk (CD-ROM), or any other form of storage medium well known in the art. An exemplary storage medium is coupled to a processor, enabling the processor to read information from and write information to the storage medium. Of course, the storage medium can also be a component of the processor. The processor and storage medium can reside in an ASIC. Furthermore, the ASIC can reside in a terminal device or management device. Alternatively, the processor and storage medium can exist as discrete components in the terminal device or management device.

[0106] Those skilled in the art will recognize that, in one or more of the examples above, the functions described in the embodiments of this application can be implemented, in whole or in part, by software, hardware, firmware, or any combination thereof. When implemented in software, it can be implemented, in whole or in part, as a computer program product. This computer program product includes one or more computer instructions. When these computer program instructions are loaded and executed on a computer, all or part of the processes or functions described in the embodiments of this application are generated. The computer can be a general-purpose computer, a special-purpose computer, a computer network, or other programmable device. The computer instructions can be stored in a computer-readable storage medium or transmitted from one computer-readable storage medium to another. For example, the computer instructions can be transmitted from one website, computer, server, or data center to another via wired (e.g., coaxial cable, fiber optic, digital subscriber line (DSL)) or wireless (e.g., infrared, wireless, microwave, etc.) means. The computer-readable storage medium can be any available medium accessible to a computer or a data storage device such as a server or data center that integrates one or more available media. The available media can be magnetic media (e.g., floppy disks, hard disks, magnetic tapes), optical media (e.g., digital video discs (DVDs)), or semiconductor media (e.g., solid-state disks (SSDs)).

[0107] The modules / units included in the various devices and products described in the above embodiments can be software modules / units, hardware modules / units, or a combination of both. For example, for devices and products applied to or integrated into a chip, all modules / units can be implemented using hardware methods such as circuits, or at least some modules / units can be implemented using software programs that run on a processor integrated within the chip, while the remaining (if any) modules / units can be implemented using hardware methods such as circuits. For devices and products applied to or integrated into a chip module, all modules / units can be implemented using hardware methods such as circuits. Different modules / units can be located in the same component (e.g., chip, circuit module, etc.) or different components of the chip module, or at least some modules / units can be implemented using hardware methods such as circuits. The implementation is achieved through a software program that runs on the processor integrated within the chip module. The remaining modules / units (if any) can be implemented using hardware methods such as circuits. For various devices and products applied to or integrated into terminal equipment, each of their modules / units can be implemented using hardware methods such as circuits. Different modules / units can be located in the same component (e.g., chip, circuit module, etc.) or different components within the terminal equipment. Alternatively, at least some modules / units can be implemented through a software program that runs on the processor integrated within the terminal equipment, while the remaining modules / units (if any) can be implemented using hardware methods such as circuits.

[0108] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the embodiments of this application. It should be understood that the above descriptions are merely specific embodiments of the embodiments of this application and are not intended to limit the protection scope of the embodiments of this application. Any modifications, equivalent substitutions, improvements, etc., made on the basis of the technical solutions of the embodiments of this application should be included within the protection scope of the embodiments of this application.

Claims

1. A multi-media integrated detection method for biotoxicity, characterized in that, The method includes: The first sample is preprocessed according to the first sample type corresponding to the first sample to obtain a liquid sample to be tested; the first sample is any one of the multi-media samples that have been collected, and the multi-media samples include at least one of the following: water sample, gaseous sample, soil sample, and solid waste sample. The liquid sample to be tested and the preset liquid control sample are quantitatively added to the sample detection chamber and control detection chamber of the first detection tube, respectively; the first detection tube is an integrated dual-chamber detection tube with the sample detection chamber and the control detection chamber being independent of each other; The liquid test sample and the liquid control sample were simultaneously mixed with bacterial solution and subjected to biochemical reaction to obtain the first target mixed sample and the second target mixed sample, respectively. For the first target mixed sample and the second target mixed sample, detection operations are performed simultaneously to obtain the first luminescence intensity data and the second luminescence intensity data, respectively; Based on the first luminescence intensity data and the second luminescence intensity data, the first detection result of the first sample is determined.

2. The method as described in claim 1, characterized in that, The step of preprocessing the first sample according to the first sample type corresponding to the first sample to obtain the liquid sample to be tested includes: If the first sample type of the first sample is the water sample, then the first sample is filtered to obtain the liquid sample to be tested; If the first sample type of the first sample is the gaseous sample, then the first sample is passed into the preset absorption liquid for gas-liquid conversion according to the preset flow rate and preset first duration to obtain the liquid sample to be tested; If the first sample type of the first sample is the soil sample or the solid waste sample, then the first sample is subjected to leaching and solid-liquid separation to obtain the liquid sample to be tested.

3. The method as described in claim 2, characterized in that, The step of extracting and separating the first sample to obtain the liquid sample to be tested includes: Obtain the sample weight corresponding to the first sample; The weight of pure water is determined based on the sample weight and the preset weight ratio; The pure water corresponding to the weight of the pure water is injected into the reaction chamber of the first sample, and the extraction process is carried out for a preset second time to obtain a reference mixture. The reference mixture is subjected to solid-liquid separation to obtain the liquid sample to be tested.

4. The method as described in claim 1, characterized in that, The liquid test sample and the liquid control sample are simultaneously mixed with bacterial solution and subjected to biochemical reactions to obtain a first target mixed sample and a second target mixed sample, respectively, including: Obtain the sample volume of the liquid sample to be tested; The bacterial culture volume is determined based on the sample volume and the preset volume ratio; The luminescent bacterial solution corresponding to the volume of the bacterial solution is uniformly mixed with the liquid test sample and the liquid control sample to obtain the first reference mixed sample and the second reference mixed sample. According to the preset ambient temperature and reaction time, the first reference mixed sample and the second reference mixed sample are controlled to carry out biochemical reactions to obtain the first target mixed sample and the second target mixed sample, respectively.

5. The method as described in claim 4, characterized in that, The step of simultaneously performing detection operations on the first target mixed sample and the second target mixed sample to obtain first luminescence intensity data and second luminescence intensity data respectively includes: The emission signals of the first target mixed sample and the second target mixed sample are collected respectively to obtain the first emission signal and the second emission signal; The first light-emitting signal and the second light-emitting signal are converted into electrical signals respectively to obtain a first reference electrical signal and a second reference electrical signal; The first reference electrical signal and the second reference electrical signal are respectively subjected to noise reduction and amplification processing to obtain the first target electrical signal and the second target electrical signal; The first target electrical signal and the second target electrical signal are converted from analog to digital to obtain the first luminous intensity data and the second luminous intensity data, respectively.

6. The method according to any one of claims 1-5, characterized in that, The step of determining the first detection result of the first sample based on the first luminescence intensity data and the second luminescence intensity data includes: Based on the first luminous intensity data and the second luminous intensity data, a first relative luminosity is determined; The first luminescence suppression rate is determined based on the first relative luminescence intensity; The first toxicity level of the first sample is determined based on the first luminescence inhibition rate; The first detection result is determined based on the first sample type, the first relative luminescence, the first luminescence inhibition rate, and the first toxicity level.

7. The method as described in claim 6, characterized in that, Determining the first toxicity level of the first sample based on the first luminescence inhibition rate includes: Obtain the sampling scenario information corresponding to the multi-media sample; the sampling scenario information includes at least one of the following: emergency detection scenario, key detection scenario, and routine detection scenario; Determine the threshold adjustment coefficient based on the sampling scenario information; Obtain a preset first reference threshold and a second reference threshold; the second reference threshold is greater than the first reference threshold. The first reference threshold and the second reference threshold are adjusted according to the threshold adjustment coefficient to obtain the first target threshold and the second target threshold; If the first emission inhibition rate is less than or equal to the first target threshold, then the first toxicity level of the first sample is determined to be low toxicity. If the first emission inhibition rate is greater than the first target threshold and less than the second target threshold, then the first toxicity level of the first sample is determined to be moderate toxicity. If the first emission inhibition rate is greater than or equal to the second target threshold, then the first toxicity level of the first sample is determined to be high toxicity.

8. A multi-media integrated biotoxicity detection device, characterized in that, The device includes a pretreatment module, a sample addition module, a mixing module, a detection module, and a determination module, wherein: The preprocessing module is used to preprocess the first sample according to the first sample type corresponding to the first sample to obtain a liquid sample to be tested; the first sample is any one of the collected multi-media samples, and the multi-media samples include at least one of the following: water sample, gaseous sample, soil sample, and solid waste sample. The sample addition module is used to quantitatively add the liquid test sample and the preset liquid control sample to the sample detection chamber and control detection chamber of the first detection tube, respectively; the first detection tube is an integrated dual-chamber detection tube with mutually independent sample detection chamber and control detection chamber; The mixing module is used to simultaneously mix the liquid test sample and the liquid control sample with bacterial solution and perform biochemical reactions to obtain a first target mixed sample and a second target mixed sample, respectively. The detection module is used to simultaneously perform detection operations on the first target mixed sample and the second target mixed sample to obtain first luminescence intensity data and second luminescence intensity data, respectively. The determining module is used to determine the first detection result of the first sample based on the first luminescence intensity data and the second luminescence intensity data.

9. An electronic device, characterized in that, include: Processor, memory, communication interface, and one or more programs; The one or more programs are stored in the memory and configured to be executed by the processor, the programs including instructions for performing the steps of the method as described in any one of claims 1-7.

10. A computer-readable storage medium, characterized in that, The computer-readable storage medium stores a computer program, the computer program including program instructions that, when executed by a processor, cause the processor to perform the method as described in any one of claims 1-7.

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