Liquid chromatograph analyzer

Abstract

The application is suitable for the field of medical equipment, and discloses a liquid chromatograph. The liquid chromatograph comprises a chromatographic column, a liquid phase fluid conveying assembly, a detector and a controller. The controller is configured to perform an elution mode matching process, that is, to control the liquid phase fluid conveying assembly to work in a preset elution mode, so as to elute and detect a first test solution made of at least a reference substance and adsorbed in the chromatographic column, and to determine a target gradient elution mode for controlling the liquid phase fluid conveying assembly to work and matching the chromatographic column according to obtained first detection information. The controller is further configured to control the liquid phase fluid conveying assembly to work in the target gradient elution mode, so as to perform gradient elution on a second test solution made of at least a sample and adsorbed in the chromatographic column, and to form a second to-be-detected solution flowing out of the chromatographic column. The application solves the problem that the sample detection result is affected due to the mismatch between the chromatographic column and the elution mode.

Description

Technical Field

[0001] This invention relates to the field of medical devices, and more particularly to a liquid chromatography analyzer. Background Technology

[0002] The related technology provides a liquid chromatography analyzer, which works on the following principle: a chromatographic column is used to adsorb a test solution made from a sample; a liquid-phase fluid delivery component is used to drive the elution phase to elute the test solution adsorbed in the chromatographic column to form a test solution; and a detector is used to detect the test solution flowing out of the chromatographic column to obtain detection information.

[0003] Chromatographic columns need to be replaced after a certain number of uses or when quality issues arise. However, due to factors such as the particle size of the packed column, the bonding density of functional groups, the packing process, the activation process, the storage process, the storage time, and the storage temperature, it is difficult to achieve high consistency between different production batches of columns. This results in variations in the retention time of the target component when eluting from different batches using the same elution method. Consequently, in practical applications, a mismatch between the column and the original elution method can easily occur after column replacement, affecting the accuracy of sample detection results. This impact is particularly pronounced in high-speed liquid chromatography analyzers. Summary of the Invention

[0004] The first objective of this invention is to provide a liquid chromatography analyzer that aims to solve the technical problem in the related art where sample detection results are affected by the mismatch between the chromatographic column and the elution method.

[0005] To achieve the above objectives, the present invention provides a liquid chromatography analyzer, comprising a chromatographic column, a liquid phase fluid delivery assembly, a detector, and a controller;

[0006] The controller is configured to execute the following elution mode matching process: controlling the liquid phase fluid delivery assembly to operate according to a preset elution mode to drive at least one elution phase through the chromatographic column, thereby eluting a first test solution prepared from at least a reference and adsorbed within the chromatographic column, thus forming a first analyte solution eluting from the chromatographic column; controlling the detector to detect the first analyte solution eluting from the chromatographic column and obtaining first detection information; and determining a target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly and matching it with the chromatographic column based on the first detection information.

[0007] The controller is also configured to perform the following elution step in the sample detection process: control the liquid phase fluid delivery assembly to operate in the target gradient elution mode to drive at least two elution phases with different ion concentrations to flow sequentially through the chromatographic column, thereby performing gradient elution on a second test solution prepared from at least the sample and adsorbed within the chromatographic column, thereby forming a second test solution flowing out of the chromatographic column.

[0008] As one implementation method, the target gradient elution method includes one of the following control methods:

[0009] The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared at different sub-target concentrations at different time periods and delivered to the chromatographic column at at least two different ion concentrations;

[0010] The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared at different time periods according to different sub-target ratio parameters and delivered to the chromatographic column.

[0011] The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared according to a target gradient elution curve and delivered to the chromatographic column.

[0012] The liquid-phase fluid delivery assembly is controlled to drive at least two elution phases with different ion concentrations, each loaded in a different storage container, to deliver one of the at least two elution phases to the chromatographic column at different time periods;

[0013] Preferably, the different sub-target ratio parameters are different sub-target volume ratios.

[0014] As one implementation, the liquid chromatograph has multiple preset gradient elution modes stored in its memory;

[0015] The step of determining the target gradient elution mode for controlling the operation of the liquid phase fluid delivery component and matching the chromatographic column based on the first detection information includes: determining one of the plurality of preset gradient elution modes as the target gradient elution mode based on the first detection information.

[0016] As one implementation, determining one of the plurality of preset gradient elution methods as the target gradient elution method based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining one of the plurality of preset gradient elution methods corresponding to the retention time of the first target component in the first test solution as the target gradient elution method based on the retention time of the first target component.

[0017] As one implementation, the liquid chromatograph has multiple preset gradient elution modes stored in its memory;

[0018] The step of determining, based on the first detection information, a target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly and matching the chromatographic column includes:

[0019] Based on the first detection information, the actual retention time of the first target component in the first test solution is obtained, and the actual retention time is compared with the target retention time range.

[0020] If the actual retention time is less than the lower limit of the target retention time range, then one of the pre-stored gradient elution methods in which the ion concentration of the elution phase used to elute the first target component during the elution process is less than the ion concentration of the elution phase used to elute the first target component during the elution process of the preset elution method is determined as the target gradient elution method.

[0021] If the actual retention time is greater than the upper limit of the target retention time range, then one of the pre-stored gradient elution methods in which the ion concentration of the elution phase used to elute the first target component during the elution process is greater than the ion concentration of the elution phase used to elute the first target component during the elution process of the preset elution method is determined as the target gradient elution method.

[0022] As one implementation, determining the target gradient elution mode for controlling the operation of the liquid-phase fluid delivery assembly and matching the chromatographic column based on the first detection information includes: obtaining elution correlation information characterizing the interaction strength between the first target component in the first test solution and the chromatographic column and the elution phase based on the first detection information; and determining the target gradient elution mode based on the elution correlation information; wherein the elution correlation information includes at least one of the following: the retention time of the first target component in the first test solution, and the chromatographic peak position of the first target component in the chromatographic curve of the first test solution;

[0023] And / or, determining the target gradient elution mode for controlling the operation of the liquid phase fluid delivery component and matching the chromatographic column based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining the target gradient elution mode based on the retention time of the first target component in the first test solution.

[0024] In one implementation, both the reference sample and the sample contain a first target component; determining the target gradient elution method for controlling the operation of the liquid phase fluid delivery assembly and matching the chromatographic column based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining the target gradient elution method based on the retention time of the first target component in the first test solution.

[0025] And / or, the components of the reference sample contain hemoglobin;

[0026] And / or, the reference material is at least one of blood sample, calibrator, and quality control material.

[0027] In one implementation, the sample is a blood sample; the controller is also configured to perform the following detection steps in the sample detection process: control the detector to detect the second test solution flowing out of the chromatographic column and obtain second detection information; and output a detection report containing at least a second target component parameter, wherein the second target component is HbA1c, based on the second detection information.

[0028] As one implementation, determining the target gradient elution method for controlling the operation of the liquid phase fluid delivery component and matching the chromatographic column based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining the target gradient elution method based on the retention time of the first target component in the first test solution;

[0029] The controller is also configured to perform the following detection steps in the sample detection process: control the detector to detect the second test solution flowing out of the chromatographic column and obtain second detection information; and output a detection report containing at least the second target component parameter based on the second detection information.

[0030] Wherein, the first target component and the second target component are the same component or are different components.

[0031] As one implementation method, the preset elution method includes one of the following control methods:

[0032] The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations and loaded in different storage containers to prepare and deliver at least two different preset ion concentrations of elution phases to the chromatographic column at different time periods, so as to perform gradient elution of the first test solution adsorbed in the chromatographic column;

[0033] The liquid phase fluid delivery assembly is controlled to drive at least two elution phases with different ion concentrations and loaded in different storage containers to deliver one of the at least two elution phases to the chromatographic column at different time periods, so as to perform gradient elution of the first test solution adsorbed in the chromatographic column;

[0034] The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations and respectively loaded in different storage containers to mix and deliver a single concentration of elution phase to the chromatographic column, so as to perform isocratic elution of the first test solution adsorbed in the chromatographic column;

[0035] The liquid phase fluid delivery assembly is controlled to drive the elution phase formed by the liquid phase fluid loaded in the storage container to deliver it to the chromatographic column in order to perform isocratic elution of the first test solution adsorbed in the chromatographic column.

[0036] In one embodiment, the liquid phase fluid delivery assembly includes a first driving device and a second driving device, wherein the first driving device is used to draw a first liquid phase fluid from a first storage container and drive the drawn first liquid phase fluid to be delivered through the chromatographic column;

[0037] The second driving device is used to draw a second liquid phase fluid with an ion concentration greater than that of the first liquid phase ion concentration from the second storage container, and drive the drawn second liquid phase fluid to flow through the chromatographic column;

[0038] The preset elution method includes one of the following control methods:

[0039] The first driving device and the second driving device are controlled to operate according to the preset elution mode, so as to drive the first liquid phase fluid and the second liquid phase fluid to prepare and deliver at least two different preset ion concentrations of elution phase to the chromatographic column at different time periods, so as to perform gradient elution of the first test solution adsorbed in the chromatographic column;

[0040] The first driving device and the second driving device are controlled to operate according to the preset elution mode, so as to drive the first liquid phase fluid and the second liquid phase fluid to mix and deliver a single concentration of elution phase to the chromatographic column, so as to perform isocratic elution of the first test solution adsorbed in the chromatographic column;

[0041] The first driving device is individually controlled to drive the first liquid phase fluid to flow into the chromatographic column, so as to perform isocratic elution of the first test solution adsorbed in the chromatographic column.

[0042] As one implementation, before controlling the liquid phase fluid delivery assembly to operate according to a preset elution mode, the controller is further configured to perform the following steps of the elution mode matching process: controlling the liquid phase fluid delivery assembly to operate according to a preset sample pushing mode to drive at least one of the elution phases to flow toward the chromatographic column, thereby pushing the first test solution, made at least from a reference, into the chromatographic column.

[0043] As one implementation, after determining the target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly and matching the chromatographic column, the controller is further configured to perform the following steps of the elution mode matching process:

[0044] The liquid phase fluid delivery assembly is controlled to operate according to the target gradient elution mode to drive at least two different ion concentrations of the elution phase to flow sequentially through the chromatographic column, thereby performing gradient elution on a third test solution prepared at least from a reference and adsorbed within the chromatographic column, thereby forming a third analyte solution flowing out of the chromatographic column;

[0045] The detector is controlled to detect the third test solution flowing out of the chromatographic column and to obtain third detection information;

[0046] Based on the third detection information, it is determined whether the retention time of the first target component in the third test solution is within the target retention time range.

[0047] In one implementation, the controller is further configured to execute the elution mode matching process before executing the calibration process;

[0048] And / or, the controller is further configured to perform the following calibration procedure: control the liquid phase fluid delivery assembly to operate according to the target gradient elution mode to drive at least two different ion concentrations of the elution phase to flow sequentially through the chromatographic column, thereby performing gradient elution on the fourth test solution, which is at least made of calibrator and adsorbed in the chromatographic column, to form a fourth test solution flowing out of the chromatographic column; control the detector to detect the fourth test solution flowing out of the chromatographic column and obtain fourth detection information; and obtain and output the calibration result based on the fourth detection information.

[0049] In one implementation, the controller is further configured to execute the elution mode matching process after acquiring information indicating that the column loading or replacement is complete, and before executing the calibration process.

[0050] And / or, the controller is further configured to execute the sample detection process after the calibration process is completed.

[0051] In one implementation, the controller is further configured to: when information indicating that the chromatographic column loading or replacement is complete is obtained, output information to prompt the execution of the elution mode matching process.

[0052] A second objective of this invention is to provide a liquid chromatograph, which includes a chromatographic column, a liquid fluid delivery assembly, a detector, and a controller.

[0053] The controller is configured to: after acquiring information indicating that the column loading or replacement is complete, and before executing the calibration procedure, execute an elution mode matching procedure to determine a target gradient elution mode that controls the operation of the liquid phase fluid delivery assembly and matches the column.

[0054] The controller is also configured to perform the following elution step in the sample detection process: control the liquid phase fluid delivery assembly to operate in the target gradient elution mode to drive at least two elution phases with different ion concentrations to flow sequentially through the chromatographic column, thereby performing gradient elution on a second test solution prepared from at least the sample and adsorbed within the chromatographic column, thereby forming a second test solution flowing out of the chromatographic column.

[0055] As one implementation, the step of performing an elution mode matching process to determine a target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly and matching the chromatographic column includes: performing an elution mode matching process to obtain the retention time of a first target component, and determining the target gradient elution mode based on the retention time of the first target component;

[0056] And / or, the target gradient elution method includes one of the following control methods:

[0057] The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared at different sub-target concentrations at different time periods and delivered to the chromatographic column as elution phases with at least two different ion concentrations.

[0058] The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared at different time periods according to different sub-target ratio parameters and delivered to the chromatographic column as elution phases with at least two different ion concentrations;

[0059] The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared according to a target gradient elution curve and delivered to the chromatographic column;

[0060] The liquid-phase fluid delivery assembly is controlled to drive at least two elution phases with different ion concentrations, each loaded in a different storage container, to deliver one of the at least two elution phases to the chromatographic column at different time periods;

[0061] Preferably, the different sub-target ratio parameters are different sub-target volume ratios.

[0062] A third objective of this invention is to provide a liquid chromatograph, which includes a chromatographic column, a liquid fluid delivery assembly, a detector, and a controller.

[0063] The controller is configured to perform the following elution mode matching process: obtain the retention time of a first target component associated with the chromatographic column, and determine a target gradient elution mode that is matched with the chromatographic column and is used to control the operation of the liquid phase fluid delivery assembly based on the retention time of the first target component;

[0064] The controller is also configured to perform the following elution step in the sample detection process: control the liquid phase fluid delivery assembly to operate in the target gradient elution mode to drive at least two elution phases with different ion concentrations to flow sequentially through the chromatographic column, thereby performing gradient elution on a second test solution prepared from at least the sample and adsorbed within the chromatographic column, thereby forming a second test solution flowing out of the chromatographic column.

[0065] In one embodiment, the liquid chromatograph further includes an identification component for identifying an identification code on the chromatographic column; obtaining the retention time of the first target component associated with the chromatographic column includes: obtaining the retention time of the first target component based on information fed back from the identification code on the chromatographic column identified by the identification component;

[0066] Alternatively, the liquid chromatograph may further include a human-machine interface component, wherein obtaining the retention time of the first target component associated with the chromatographic column includes: obtaining the retention time of the first target component based on information input by the operator through the human-machine interface component.

[0067] The liquid chromatography analyzer provided by this invention performs an elution mode matching process on a chromatographic column before executing a sample detection procedure. This process determines a target gradient elution mode that matches the column. Specifically, the column and a preset elution mode are used to elute and detect a first sample solution, which is at least made from a reference and adsorbed within the column. Based on the first detection information obtained, a target gradient elution mode matching the column is determined. Then, the same target gradient elution mode is used to elute and detect a second sample solution, which is at least made from a sample and adsorbed within the column. Because the target gradient elution mode used in the sample detection procedure is a matching elution mode determined through the elution mode matching process, it helps to avoid adverse phenomena caused by mismatch between the column and the elution mode affecting the sample detection results. This ensures that the liquid chromatography analyzer can obtain accurate detection results when using different batches of chromatographic columns to elute and detect samples. Attached Figure Description

[0068] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0069] Figure 1 This is a schematic diagram of the liquid path of the liquid chromatograph provided in an embodiment of the present invention;

[0070] Figure 2 This is a three-dimensional schematic diagram of the liquid chromatography analyzer provided in an embodiment of the present invention.

[0071] Figure 3 This is a schematic diagram of the retention times of HbA1c and HbA0 in a chromatogram provided in an embodiment of the present invention.

[0072] Reference numerals: 100, Liquid Chromatography Analyzer; 110, Chromatographic Column; 120, Liquid Fluid Delivery Assembly; 121, First Actuation Device; 122, Second Actuation Device; 123, Damping Device; 130, Detector; 140, Sample Preparation Channel; 150, Sample Processing Assembly; 151, Sampling Component; 152, Reaction Vessel; 153, Sample Delivery Path; 1531, Suction / Expulsion Power Component; 160, Reversing Valve; 170, Display Screen; 180, Housing; 190, First Waste Channel; 200, First Storage Container; 300, Second Storage Container; 400, Hemolysin Container. Detailed Implementation

[0073] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0074] The solution provided in this invention is applicable to liquid chromatography analyzers that use gradient elution to elute and detect samples, thereby solving the technical problem in related technologies where sample detection results are affected due to mismatch between the chromatographic column and the gradient elution method.

[0075] Gradient elution refers to the process of changing the concentration of the eluent phase according to a preset program within the same detection cycle to use different concentrations of eluent phase at different times. Using gradient elution in a liquid chromatography analyzer can optimize the elution conditions of each component, improve separation efficiency, shorten the detection cycle, and improve peak shape. In this embodiment, the concentration of the eluent phase refers to the concentration of the liquid fluid with a high ion concentration (such as the second liquid fluid mentioned below) in the eluent phase, or the ion concentration in the eluent phase.

[0076] Reference Figure 1 As shown, a first aspect of this invention provides a liquid chromatograph 100. The liquid chromatograph 100 includes a chromatographic column 110, a liquid phase delivery assembly 120, a detector 130, and a controller (not shown). The liquid phase delivery assembly 120 drives the eluent phase through the chromatographic column 110. The chromatographic column 110 is used to adsorb a test solution prepared from a sample, and to elute the test solution with the eluent phase driven by the liquid phase delivery assembly 120 to form a analyte. The detector 130 detects the analyte eluent from the chromatographic column 110 and obtains detection information. The controller is configured to obtain the detection result of the sample based on the detection information. The eluent phase, also known as the eluent, is used to elute the test solution adsorbed on the chromatographic column 110, so that the detector 130 can detect the different components eluted from the chromatographic column 110. The liquid chromatograph 100 is an instrument used for chromatographic analysis of samples. Its sample detection process includes: delivering a test solution prepared from the sample to the chromatographic column 110, then driving the eluent phase through the chromatographic column 110 to gradually elute and separate the different components of the test solution adsorbed on the chromatographic column 110, detecting the test solution flowing out of the chromatographic column 110, analyzing the detection information, and obtaining the sample detection result.

[0077] In one implementation, the controller is also configured to execute an elution mode matching process to determine a target gradient elution mode that controls the operation of the liquid chromatography fluid delivery assembly 120 and matches the chromatographic column 110; the controller is also configured to execute the elution step in the sample detection process using the target gradient elution mode. The elution mode matching process is mainly used to determine the target gradient elution mode that matches the chromatographic column 110. Specifically, the elution mode matching process involves performing one elution detection process using the chromatographic column 110 to obtain a target gradient elution mode that matches the chromatographic column 110. Since the target gradient elution mode used in the sample detection process is the elution mode determined by the elution mode matching process and matches the chromatographic column 110, it helps to avoid the adverse phenomenon that the sample detection results are affected due to the mismatch between the chromatographic column 110 and the elution mode. This helps to ensure that the liquid chromatography analyzer 100 can obtain accurate detection results when using different chromatographic columns 110 (especially chromatographic columns 110 from different production batches) to perform elution detection on samples.

[0078] In one implementation, the controller is configured to perform the following elution mode matching process: controlling the liquid phase fluid delivery assembly 120 to operate according to a preset elution mode to drive at least one elution phase through the chromatographic column 110, thereby eluting a first test solution prepared from at least a reference and adsorbed within the chromatographic column 110, thus forming a first analyte eluting from the chromatographic column 110; controlling the detector 130 to detect the first analyte eluting from the chromatographic column 110 and obtaining first detection information; and determining a target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly 120 and matching it with the chromatographic column 110 based on the first detection information. The controller is also configured to perform the following elution step in the sample detection process: controlling the liquid phase fluid delivery assembly 120 to operate according to a target gradient elution mode to drive at least two elution phases of different ion concentrations sequentially through the chromatographic column 110, thereby gradient eluting a second test solution prepared from at least a sample and adsorbed within the chromatographic column 110, thus forming a second analyte eluting from the chromatographic column 110. The higher the ion concentration of the elution phase, the greater the corresponding ionic strength. The elution mode matching process is mainly used for eluting and detecting reference samples to obtain a target gradient elution mode that matches the chromatographic column 110. The reference sample can be an item of the same type as the sample, or an item of a different type but with one or more of the same components as the sample. The preset elution mode is a elution mode pre-stored in the liquid chromatograph 100 for executing the elution mode matching process; it can be an isocratic elution mode or a gradient elution mode. The sample detection process is mainly used for eluting and detecting samples to obtain the chromatographic analysis results of the samples. In this embodiment, since the target gradient elution mode used in the sample detection process is the elution mode that matches the chromatographic column 110, determined by executing the elution mode matching process with the reference sample, it effectively ensures that the elution mode used in the sample detection process matches the chromatographic column 110, thereby avoiding the adverse phenomenon of sample detection results being affected due to mismatch between the chromatographic column 110 and the elution mode.

[0079] In one implementation, in the elution mode matching process, driving at least one eluent phase made of at least one liquid fluid through the chromatographic column 110 includes: driving at least one eluent phase made of at least one liquid fluid through the chromatographic column 110. In the following elution step in the sample detection process, driving at least two eluent phases with different ion concentrations sequentially through the chromatographic column 110 includes: driving at least two eluent phases with different ion concentrations made of at least two liquid fluids with different ion concentrations sequentially through the chromatographic column 110. The liquid chromatograph 100 is equipped with at least two reservoirs, each reservoir being used to load one liquid fluid. The liquid fluid delivery assembly 120 is used to draw liquid fluid from one reservoir or at least two reservoirs and deliver it to the chromatographic column 110 under the control of a controller. When the liquid fluid delivery assembly 120 draws liquid fluid from only one reservoir and delivers it to the chromatographic column 110, the eluent phase is formed by that single liquid fluid. When the liquid phase fluid delivery assembly 120 simultaneously draws liquid phase fluid from at least two reservoirs and delivers it to the chromatographic column 110, the eluent phase is formed by mixing the at least two liquid phase fluids. In this embodiment, the various eluent phases used in the preset elution mode and the target gradient elution mode are prepared online using liquid phase fluid. Thus, a single set of liquid phase fluids composed of at least two liquid phase fluids can meet the different elution detection requirements of the liquid chromatograph 100, without the need to configure a separate reservoir for each concentration of eluent phase, thereby reducing material volume and material costs. Of course, in an alternative embodiment, the liquid chromatograph 100 can also be directly equipped with multiple reservoirs containing different concentrations of eluent phase. At a specific moment or time period, the eluent phase can be directly drawn from one reservoir and delivered to the chromatographic column 110 without the need for online preparation of the eluent phase. In this alternative embodiment, the above-mentioned driving of at least one eluent phase made of at least one liquid fluid through the chromatographic column 110 includes: driving at least one eluent phase loaded in a reservoir container through the chromatographic column 110; in the following elution step in the sample detection process, the above-mentioned driving of at least two eluent phases with different ion concentrations through the chromatographic column 110 in sequence includes: driving at least two eluent phases with different ion concentrations, respectively loaded in different reservoir containers, through the chromatographic column 110 in sequence.

[0080] Gradient elution methods include step gradient elution, linear gradient elution, and irregular gradient elution.

[0081] Among them, the stepped gradient elution method is a segmented and rapid switching of elution phase concentration. The concentration of the elution phase in two adjacent time periods changes instantaneously in a step-like manner. For example, one implementation of the stepped gradient elution method includes: in the first time period, the second time period, and the third time period set in sequence, the concentration of the elution phase in the first time period is kept constant at the first concentration, the concentration of the elution phase in the second time period is kept constant at the second concentration, and the concentration of the elution phase in the third time period is kept constant at the third concentration. The end time of the first time period is the same as the start time of the second time period, the end time of the second time period is the same as the start time of the third time period, and the second concentration is greater than the first concentration and less than the third concentration.

[0082] Linear gradient elution is an elution method in which the concentration of the elution phase increases linearly with time, and the concentration of the elution phase changes linearly between two adjacent time periods.

[0083] Irregular gradient elution is a non-step and non-linear gradient elution method. For example, one implementation of irregular gradient elution includes: in a first time period, a second time period, a third time period, and a fourth time period set sequentially, the concentration of the eluted phase in the first time period increases linearly to a first concentration according to a first slope; the concentration of the eluted phase in the second time period remains constant at the first concentration; the concentration of the eluted phase in the third time period increases linearly to a second concentration according to a second slope; the concentration of the eluted phase in the fourth time period remains constant at the second concentration; the end time of the first time period is the same as the start time of the second time period; the end time of the second time period is the same as the start time of the third time period; the end time of the third time period is the same as the start time of the fourth time period; the second slope is the same as or different from the first slope; and the second concentration is greater than the first concentration.

[0084] In the liquid chromatograph 100 of this embodiment, the target gradient elution method is either irregular gradient elution or step gradient elution.

[0085] As one implementation method, the target gradient elution method includes one of the following control methods: controlling the liquid phase fluid delivery component 120 to drive at least two liquid phase fluids with different ion concentrations and loaded in different storage containers to prepare and deliver at least two different ion concentrations of elution phase to the chromatographic column 110 at different time periods according to different sub-target concentrations (here, the concentration can be the volume percentage concentration of a certain liquid phase fluid in the elution phase, or the volume percentage concentration of ions in the elution phase); controlling the liquid phase fluid delivery component 120 to drive at least two different ion concentrations of liquid phase fluids and loaded in different storage containers to prepare and deliver at least two different ion concentrations of elution phase to the chromatographic column 110 at different time periods according to different sub-target ratio parameters; controlling the liquid phase fluid delivery component 120 to drive at least two different ion concentrations of liquid phase fluids and loaded in different storage containers to prepare and deliver at least two different ion concentrations of elution phase according to the target gradient elution curve. In this embodiment, the multiple eluent phases with different ion concentrations used in the target gradient elution method can be prepared by controlling the concentration, by controlling the mixing ratio parameters of different liquid phase fluids, or by controlling the gradient elution curve. That is, the eluent phases used for gradient elution are prepared online, and parameters such as concentration, mixing ratio, or gradient elution curve are adjusted in real time by a controller. Alternatively, in an alternative embodiment, the eluent phases used for gradient elution may not be prepared online. Instead, multiple storage containers containing different concentrations of eluent phases are directly installed in the liquid chromatograph 100. In this alternative embodiment, the target gradient elution method includes the following control method: controlling the liquid phase fluid delivery component 120 to drive at least two eluent phases with different ion concentrations, each loaded in a different storage container, to deliver one of these at least two eluent phases to the chromatographic column 110 at different time periods.

[0086] In one implementation, the aforementioned control liquid-phase fluid delivery component 120 drives at least two liquid phases with different ion concentrations, each loaded in a different reservoir, to prepare and deliver elution phases of at least two different ion concentrations to the chromatographic column 110 at different time periods. This includes: controlling the operation of the liquid-phase fluid delivery component 120 at different time periods within the elution cycle of the sample using multiple sub-target concentrations, so that each time period can prepare an elution phase with a corresponding sub-target concentration, and delivering the elution phase to the chromatographic column 110. Specifically, in the elution control program corresponding to the target gradient elution mode, the elution cycle of the sample is divided into multiple time periods (which can be a period of time or a moment) according to the different sub-target concentrations of the elution phase, and the sub-target concentration of the elution phase in each time period is kept constant, while the sub-target concentrations of the elution phase in adjacent time periods are different from each other. In this way, when executing the elution control program corresponding to the target gradient elution mode, the liquid-phase fluid delivery component 120 can be controlled to prepare an elution phase with the corresponding sub-target concentration in the corresponding time period and deliver the elution phase to the chromatographic column 110. In this embodiment, the various elution phases with different ion concentrations used in the target gradient elution method are prepared by controlling the concentration. That is, the elution control program is configured with a sub-target concentration of the elution phase corresponding to each time period. For example, the control principle is illustrated by using elution phases with three sub-target concentrations in one elution cycle: In the first time period, the liquid phase fluid delivery component 120 is controlled to prepare the elution phase with the first sub-target concentration and delivers the elution phase to the chromatographic column 110; in the second time period, the liquid phase fluid delivery component 120 is controlled to prepare the elution phase with the second sub-target concentration and delivers the elution phase to the chromatographic column 110; in the third time period, the liquid phase fluid delivery component 120 is controlled to prepare the elution phase with the third sub-target concentration and delivers the elution phase to the chromatographic column 110.

[0087] In one implementation, the aforementioned different sub-target ratio parameters are different sub-target volume ratios, that is: the aforementioned control liquid phase fluid delivery component 120 drives at least two liquid phase fluids with different ion concentrations and respectively loaded in different storage containers to be prepared according to different sub-target ratio parameters at different time periods and deliver at least two different ion concentrations of elution phase to the chromatographic column 110, including: controlling the liquid phase fluid delivery component 120 to drive at least two liquid phase fluids with different ion concentrations and respectively loaded in different storage containers to be prepared according to different sub-target volume ratios at different time periods and deliver at least two different ion concentrations of elution phase to the chromatographic column 110.

[0088] In one implementation, the aforementioned control liquid phase fluid delivery component 120 drives at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to prepare and deliver at least two different ion concentration elution phases to the chromatographic column 110 at different time periods according to different sub-target volume ratios. This includes: controlling the operation mode of the liquid phase fluid delivery component 120 at different time periods according to multiple sub-target volume ratios of the at least two liquid phase fluids during the elution cycle of the sample, so that each time period can drive at least two liquid phase fluids to prepare elution phases with corresponding sub-target ion concentrations according to different sub-target volume ratios, and delivering the elution phases to the chromatographic column 110. Specifically, in the elution control program corresponding to the target gradient elution mode, the elution cycle of the sample is divided into multiple time periods (which can be a period of time or a moment) according to the different multiple sub-target volume ratios of the at least two liquid phase fluids, and the multiple sub-target volume ratios of the at least two liquid phase fluids are constant in each time period, while the multiple sub-target volume ratios of the at least two liquid phase fluids in adjacent time periods are different from each other. Thus, when executing the elution control program corresponding to the target gradient elution mode, the liquid phase fluid delivery component 120 can be controlled to prepare the elution phase using the corresponding sub-target volume ratio within the corresponding time period, and the elution phase can be delivered to the chromatographic column 110. In this embodiment, the multiple elution phases with different ion concentrations used in the target gradient elution mode are prepared by controlling the volume ratio of at least two liquid phase fluids; that is, the elution control program is configured with the sub-target volume ratio of at least two liquid phase fluids corresponding to each time period. For example, the control principle is illustrated by using an elution phase with three sub-target volume ratios within one elution cycle: In the first time period, the liquid phase fluid delivery assembly 120 is controlled to prepare a first elution phase according to the first sub-target volume ratio of at least two liquid phase fluids, and the first elution phase is delivered to the chromatographic column 110; in the second time period, the liquid phase fluid delivery assembly 120 is controlled to prepare a second elution phase according to the second sub-target volume ratio of at least two liquid phase fluids, and the second elution phase is delivered to the chromatographic column 110; in the third time period, the liquid phase fluid delivery assembly 120 is controlled to prepare a third elution phase according to the third sub-target volume ratio of at least two liquid phase fluids, and the third elution phase is delivered to the chromatographic column 110.

[0089] In one implementation, the aforementioned controlled liquid-phase fluid delivery assembly 120 drives at least two liquid phases with different ion concentrations, each loaded in a different reservoir, to generate and deliver elution phases of at least two different ion concentrations to the chromatographic column 110 according to a target gradient elution curve. This includes: controlling the operation of the liquid-phase fluid delivery assembly 120 at different time periods within the elution cycle of the sample according to the target gradient elution curve, so that each time period can generate an elution phase with a corresponding sub-target ion concentration, and delivering the elution phase to the chromatographic column 110. The target gradient elution curve is a gradient elution curve plotted with time as the abscissa and concentration or volume ratio as the ordinate.

[0090] As one implementation, the above-mentioned determination of the target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly 120 and matching it with the chromatographic column 110 based on the first detection information includes: obtaining elution correlation information to characterize the interaction strength between the first target component in the first test solution and the chromatographic column 110 and the elution phase based on the first detection information; and determining the target gradient elution mode based on the elution correlation information. The elution separation effect and separation efficiency of the first target component in the chromatographic column 110 are strongly correlated with both the chromatographic column 110 and the elution phase. Therefore, in this implementation, during the execution of the elution mode matching process, determining the target gradient elution mode based on the elution correlation information of the interaction strength between the first target component and the chromatographic column 110 and the elution phase helps to ensure that the target gradient elution mode can accurately match the chromatographic column 110.

[0091] In one implementation, the elution correlation information includes at least one of the following: the retention time of the first target component in the first test solution, and the chromatographic peak position of the first target component in the chromatogram of the first test solution. The retention time of a component specifically refers to the time elapsed from the start of sample injection to the detection of the maximum concentration of that component, i.e., the time required from sample injection to the occurrence of the maximum response (i.e., the peak apex) of that component in the detector 130, which is equal to the interval between the chromatographic peak position of that component and the origin in the chromatogram. For example, the retention times of HbA1c and HbA0 are illustrated in the chromatogram as follows: Figure 3As shown, the chromatographic peak position of a certain component is the location of the apex of the chromatographic peak of that component in the chromatographic curve. Both the retention time and the chromatographic peak position of the first target component can characterize the interaction strength between the first target component and the chromatographic column 110 and the elution phase. In this embodiment, during the execution of the elution mode matching process, based on the first detection information, the retention time and / or the chromatographic peak position of the first target component are first obtained, and then the target gradient elution mode is determined based on the retention time and / or the chromatographic peak position of the first target component. Using this method to determine the target gradient elution mode is simple to implement and can ensure the matching degree between the target gradient elution mode and the chromatographic column 110.

[0092] In one implementation, the elution correlation information is the retention time of the first target component in the first test solution. That is, determining the target gradient elution method for controlling the operation of the liquid phase fluid delivery assembly 120 and matching the chromatographic column 110 based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining the target gradient elution method based on the retention time of the first target component in the first test solution. The retention time of the first target component is relatively easy to obtain based on the first detection information; and the retention time of the first target component can effectively characterize the interaction strength between the first target component and the chromatographic column 110 and the elution phase. Using the retention time of a specific component to determine the target gradient elution method helps ensure the consistency of the retention times of the components obtained when eluting and detecting the sample using this chromatographic column 110 and the target gradient elution method with those obtained using other chromatographic columns 110 and corresponding gradient elution methods, thereby effectively reducing the occurrence of adverse phenomena caused by mismatch between the chromatographic column 110 and the elution method affecting the sample detection results.

[0093] In one implementation, the liquid chromatograph 100 pre-stores multiple preset gradient elution modes. The aforementioned determination of the target gradient elution mode for controlling the operation of the liquid phase fluid delivery component 120 and matching the chromatographic column 110 based on the first detection information includes: determining one of the multiple preset gradient elution modes as the target gradient elution mode based on the first detection information. The multiple preset gradient elution modes are pre-stored in the liquid chromatograph 100 as program modules. When a preset gradient elution mode is determined as the target gradient elution mode, during the sample detection process, the program module corresponding to that preset gradient elution mode can be directly called to control the operation of the liquid phase fluid delivery component 120. In this implementation, by pre-stored multiple preset gradient elution modes in the liquid chromatograph 100, and selecting one of the preset gradient elution modes as the target gradient elution mode based on the first detection information obtained from executing the elution mode matching process, the control method is simple and easy to implement.

[0094] As one implementation, determining one of a plurality of preset gradient elution methods as the target gradient elution method based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information; and determining one of a plurality of preset gradient elution methods corresponding to the retention time of the first target component in the first test solution as the target gradient elution method based on the retention time of the first target component. In this implementation, during the execution of the elution method matching process, the retention time of the first target component is first obtained based on the first detection information, and then the target gradient elution method is determined based on the retention time of the first target component. For example, in a specific application, the retention time of the first target component can be divided into multiple target intervals according to a preset duration, and a corresponding preset gradient elution method can be set for each target interval. When the retention time of the first target component is obtained according to the elution method matching process, the target interval in which the retention time is located is determined, and then the preset gradient elution method corresponding to the target interval is determined as the target gradient elution method.

[0095] The above scheme establishes a one-to-one correspondence between multiple target intervals and multiple preset gradient elution modes in the liquid chromatograph 100 beforehand. During the elution mode matching process, after obtaining the retention time of the first target component, the target interval containing that retention time is determined, and then the preset gradient elution mode associated with that target interval is used as the target gradient elution mode. This scheme for determining the target gradient elution mode is simple to implement and ensures a good match between the target gradient elution mode and the chromatographic column 110, effectively preventing adverse phenomena such as sample detection results being affected by mismatch between the chromatographic column 110 and the elution mode.

[0096] Of course, in specific applications, the methods for determining the target gradient elution method are not limited to the above schemes. For example, as an alternative implementation, the liquid chromatograph 100 has multiple preset gradient elution modes stored in its memory. The determination of the target gradient elution mode for controlling the operation of the liquid flow delivery assembly 120 and matching the chromatographic column 110 based on the first detection information includes: obtaining the actual retention time of the first target component in the first test solution based on the first detection information; comparing the actual retention time with a target retention time range; if the actual retention time is less than the lower limit of the target retention time range, then determining a stored gradient elution mode among the multiple preset gradient elution modes where the ion concentration of the eluent used to elute the first target component during the elution process is less than the ion concentration of the eluent used to elute the first target component during the elution process of the preset elution mode, as the target gradient elution mode; if the actual retention time is greater than the upper limit of the target retention time range, then determining a stored gradient elution mode among the multiple preset gradient elution modes where the ion concentration of the eluent used to elute the first target component during the elution process is greater than the ion concentration of the eluent used to elute the first target component during the elution process of the preset elution mode, as the target gradient elution mode. In this alternative implementation, instead of establishing a one-to-one correspondence between multiple target intervals and multiple preset gradient elution modes in the liquid chromatograph 100, a target retention time range is pre-stored. During the elution mode matching process, after obtaining the actual retention time of the first target component, the actual retention time is compared with the target retention time range, and the target gradient elution mode is determined based on the comparison result. The principle for determining the target gradient elution mode based on the comparison result is as follows: if the actual retention time is too large (greater than the upper limit of the target retention time range), the concentration of the eluent phase used to elute the first target component is increased (this concentration is the concentration of the liquid phase fluid with a high ion concentration in the eluent phase, or the ion concentration); if the actual retention time is too small (less than the lower limit of the target retention time range), the concentration of the eluent phase used to elute the first target component is decreased. This facilitates adjusting the retention time of the first target component to within the target retention time range, so that the chromatographic peak of the first target component is maintained or fixed within the interval corresponding to the target retention time range.

[0097] As one implementation method, the preset elution method is either a preset gradient elution method or a preset isocratic elution method. That is, the preset elution method used to elute the reference sample in the elution method matching process can be either gradient elution or isocratic elution.

[0098] As one implementation method, the preset elution method includes one of the following control methods: controlling the liquid phase fluid delivery assembly 120 to drive at least two liquid phase fluids with different ion concentrations and loaded in different storage containers to prepare and deliver at least two different preset ion concentrations of elution phases to the chromatographic column 110 at different time periods, so as to perform gradient elution of the first sample solution adsorbed in the chromatographic column 110; controlling the liquid phase fluid delivery assembly 120 to drive at least two different ion concentrations of elution phases and loaded in different storage containers to deliver at least two preset ion concentrations of elution phases to the chromatographic column 110 at different time periods. One type of elution phase is used to perform gradient elution of a first sample adsorbed within a chromatographic column 110; a liquid phase delivery assembly 120 is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different reservoir, to mix and deliver a single-concentration elution phase to the chromatographic column 110 for isocratic elution of the first sample adsorbed within the chromatographic column 110; the liquid phase delivery assembly 120 is also controlled to drive the elution phase formed by a liquid phase fluid loaded in a reservoir to deliver it to the chromatographic column 110 for isocratic elution of the first sample adsorbed within the chromatographic column 110. In this embodiment, when the preset elution method is gradient elution, the elution phase used in the gradient elution process can be prepared by online mixing of liquid phase fluids or by offline preparation. When the preset elution method is isocratic elution, the elution phase used in the isocratic elution process can be prepared by online mixing of at least two liquid phase fluids or by a single liquid phase fluid.

[0099] In one implementation, the liquid chromatograph 100 is configured with two types of liquid phase fluids, namely, the aforementioned at least two types of liquid phase fluids include a first liquid phase fluid and a second liquid phase fluid. The liquid chromatograph 100 is provided with a first storage chamber and a second storage chamber. The first storage chamber is used to house a first reservoir 200 containing the first liquid phase fluid for loading the first liquid phase fluid, and the second storage chamber is used to house a second reservoir 300 containing the second liquid phase fluid for loading the second liquid phase fluid. A liquid phase fluid delivery assembly 120 is used to draw the first liquid phase fluid from the first reservoir 200 and deliver it to the chromatographic column 110, and to draw the second liquid phase fluid from the second reservoir 300 and deliver it to the chromatographic column 110. The first liquid phase fluid may specifically be liquid A, and the second liquid phase fluid may specifically be liquid B. The aforementioned different concentrations of eluent phases may refer to eluent phases with different concentrations of the second liquid phase fluid, or to eluent phases with different ion concentrations. In this embodiment, two liquid phase fluids can be used to prepare multiple eluent phases of different concentrations online to meet the requirements of the liquid chromatograph 100 for the eluent phase, and this also helps to reduce the amount of material. Of course, in specific applications, as an alternative embodiment, the liquid chromatograph 100 can be configured with three, four or more liquid phase fluids.

[0100] In one implementation, the ionic strength of the first liquid phase fluid is less than that of the second liquid phase fluid. Ionic strength is a measure of the ion concentration in a solution and is a function of the concentration of all ions in the solution. When ionic compounds dissolve in a solvent, they dissociate into ions. The concentration of electrolytes in the solution affects the solubility of other salts, and the strength of this effect is called ionic strength. In this embodiment, setting the ionic strength of the first liquid phase fluid to be less than that of the second liquid phase fluid makes the elution capacity of the first liquid phase fluid weaker than that of the second liquid phase fluid.

[0101] In one implementation, the first liquid phase fluid and the second liquid phase fluid satisfy at least one of the following conditions: the ion concentration of the first liquid phase fluid is less than the ion concentration of the second liquid phase fluid; the concentration of the buffer salt in the first liquid phase fluid is less than the concentration of the buffer salt in the second liquid phase fluid; and the pH value of the first liquid phase fluid is less than the pH value of the second liquid phase fluid. Buffer salts are an important component of liquid phase fluids. If the concentration of the buffer salt in the liquid phase fluid is high, and / or the ion concentration is high, and / or the pH value is high, the ionic strength and elution capacity of the liquid phase fluid will be relatively greater. Therefore, by setting the first liquid phase fluid and the second liquid phase fluid to satisfy at least one of the above conditions, the purpose of making the ionic strength and elution capacity of the first liquid phase fluid less than that of the second liquid phase fluid can be achieved.

[0102] Reference Figure 1 As shown, in one embodiment, the liquid phase fluid delivery assembly 120 includes a first driving device 121 and a second driving device 122. The first driving device 121 is used to draw a first liquid phase fluid from the first storage container 200 and drive the drawn first liquid phase fluid to flow through the chromatographic column 110. The second driving device 122 is used to draw a second liquid phase fluid with an ion concentration greater than that of the first liquid phase fluid from the second storage container 300 and drive the drawn second liquid phase fluid to flow through the chromatographic column 110. The first driving device 121 can drive the first liquid phase fluid to flow through the chromatographic column 110 independently, and the second driving device 122 can drive the second liquid phase fluid to flow through the chromatographic column 110 independently. In specific applications, by adjusting the driving parameters of the first driving device 121 and the second driving device 122, elution phases containing different concentrations of the second liquid phase fluid can be prepared online, thereby meeting the design requirements of the aforementioned preset elution mode and target gradient elution mode.

[0103] In one implementation, the number of first driving devices 121 and second driving devices 122 is single, meaning that both the first and second liquid phase fluids are driven by a single power source to be transported toward the chromatographic column 110. This reduces the number of power sources in the liquid chromatograph 100, thereby effectively reducing the cost of the liquid chromatograph 100 and facilitating a reduction in its size. Of course, in specific applications, as an alternative implementation, the liquid phase fluid delivery assembly 120 may also be provided with two first driving devices 121 and / or two second driving devices 122 arranged in parallel.

[0104] Reference Figure 1 As shown, in one embodiment, the liquid phase fluid delivery assembly 120 further includes a damping device 123. The damping device 123 is disposed between the first driving device 121 and the chromatographic column 110 along the flow direction of the first liquid phase fluid to buffer pressure changes and / or flow rate changes during the flow of the first liquid phase fluid. By providing the damping device 123, this embodiment can eliminate flow rate fluctuations and / or pressure fluctuations caused by intermittent suction and discharge of the first driving device 121, thereby making the flow rate and pressure of the liquid phase fluid in the liquid circuit system tend to be stable. In the operation of the liquid chromatograph 100, the amount of the first liquid fluid used is greater than that of the second liquid fluid, and the number of suction and discharge actions performed by the first driving device 121 is greater than that performed by the second driving device 122. The damping device 123 is set separately to buffer the pressure and / or flow rate fluctuations during the flow of the first liquid fluid, which can make the operation of the liquid circuit system tend to be stable. Therefore, it is not necessary to set up an additional damping device 123 to buffer the pressure and / or flow rate fluctuations during the flow of the second liquid fluid. Thus, the cost of the liquid chromatograph 100 can be reduced while ensuring that the flow rate and pressure of the liquid circuit system tend to be stable.

[0105] In one implementation, both the first driving device 121 and the second driving device 122 are high-pressure driving devices, meaning that both the first driving device 121 and the second driving device 122 can individually output a high-pressure liquid fluid, so that the liquid fluid has a sufficiently high pressure to flow through the chromatographic column 110. Specifically, the liquid fluid requires a high pressure to flow through the chromatographic column 110. The first driving device 121 can drive the first liquid fluid to flow through the chromatographic column 110 at a pressure greater than or equal to 2 MPa and less than or equal to 10 MPa, and the second driving device 122 can drive the second liquid fluid to flow through the chromatographic column 110 at a pressure greater than or equal to 2 MPa and less than or equal to 10 MPa. That is, the target pressure values ​​of the first liquid fluid and the second liquid fluid are both between 2 MPa and 10 MPa.

[0106] In one embodiment, the first driving device 121 includes a first motor, a first plunger pump, and a first screw drive mechanism. The first plunger pump includes a first cylinder and a first plunger. The first plunger is at least partially movably disposed within the first cylinder. The first screw drive mechanism is tractively connected between the first motor and the first plunger to drive the first plunger to reciprocate under the drive of the first motor.

[0107] In one embodiment, the second driving device 122 includes a second motor, a second plunger pump, and a second lead screw transmission mechanism. The second plunger pump includes a second cylinder and a second plunger. The second plunger is at least partially movably disposed within the second cylinder. The second lead screw transmission mechanism is tractively connected between the second motor and the second plunger to drive the second plunger to reciprocate under the drive of the second motor.

[0108] As one implementation method, the preset elution mode includes one of the following control methods: controlling the first driving device 121 and the second driving device 122 to operate according to the preset elution mode, so as to drive the first liquid phase fluid and the second liquid phase fluid to prepare and deliver at least two different preset ion concentrations of elution phase to the chromatographic column 110 at different time periods, so as to perform gradient elution of the first test solution adsorbed in the chromatographic column 110; controlling the first driving device 121 and the second driving device 122 to operate according to the preset elution mode, so as to drive the first liquid phase fluid and the second liquid phase fluid to mix and prepare and deliver a single concentration of elution phase to the chromatographic column 110, so as to perform isocratic elution of the first test solution adsorbed in the chromatographic column 110; or controlling the first driving device 121 alone to drive the first liquid phase fluid to flow into the chromatographic column 110, so as to perform isocratic elution of the first test solution adsorbed in the chromatographic column 110. The elution phase used in the preset elution mode is implemented by the controller controlling the operation of the first driving device 121 and the second driving device 122 according to a preset program. In this embodiment, when the preset elution method is gradient elution, the eluent used in the gradient elution process is prepared by online mixing of a first liquid phase fluid and a second liquid phase fluid. When the preset elution method is isocratic elution, the eluent used in the isocratic elution process can be prepared by online mixing of a first liquid phase fluid and a second liquid phase fluid, or it can be formed solely by the first liquid phase fluid.

[0109] As one implementation method, the target gradient elution method includes one of the following control methods: controlling the first driving device 121 and the second driving device 122 to drive the first liquid phase fluid and the second liquid phase fluid to prepare and deliver at least two different ion concentrations of eluent to the chromatographic column 110 at different time periods according to different sub-target concentrations; controlling the first driving device 121 and the second driving device 122 to drive the first liquid phase fluid and the second liquid phase fluid to prepare and deliver at least two different ion concentrations of eluent to the chromatographic column 110 at different time periods according to different sub-target ratio parameters; controlling the first driving device 121 and the second driving device 122 to drive the first liquid phase fluid and the second liquid phase fluid to prepare and deliver at least two different ion concentrations of eluent to the chromatographic column 110 according to the target gradient elution curve. In specific applications, by controlling the operating parameters of the first driving device 121 and the second driving device 122 according to the control program corresponding to the target gradient elution method, different concentrations of eluent can be prepared and delivered to the chromatographic column 110 at different time periods, thereby realizing the function of eluting the second sample solution adsorbed on the chromatographic column 110 using the target gradient elution method.

[0110] In one embodiment, before the liquid phase fluid delivery assembly 120 operates according to the preset elution mode, the controller is further configured to perform the following steps of the elution mode matching process: controlling the liquid phase fluid delivery assembly 120 to operate according to the preset sample pushing mode to drive at least one elution phase to flow toward the chromatographic column 110, thereby pushing the first test solution prepared from at least the reference into the chromatographic column 110. In this embodiment, the first test solution prepared from the reference is pushed to the chromatographic column 110 by the elution phase driven by the liquid phase fluid delivery assembly 120, that is, the liquid phase fluid delivery assembly 120 is time-division multiplexed for driving the test solution and driving the elution phase for eluting the test solution.

[0111] In one implementation, when the liquid-phase fluid delivery assembly 120 operates according to a preset push method, the aforementioned driving at least one elution phase to flow toward the chromatographic column 110, thereby pushing a first test solution prepared from at least a reference sample into the chromatographic column 110, includes: driving a liquid-phase fluid to flow toward the chromatographic column 110, thereby pushing a first test solution prepared from at least a reference sample into the chromatographic column 110. Since the preset push method is mainly used to push the first test solution to the chromatographic column 110 by a fluid with a certain pressure, driving only one liquid-phase fluid to flow toward the chromatographic column 110 is sufficient to meet the requirements and helps to reduce the control complexity of the liquid-phase fluid delivery assembly 120.

[0112] In one embodiment, the above-described method of driving a liquid fluid to flow toward the chromatographic column 110, thereby propelling a first test solution prepared from at least a reference sample into the chromatographic column 110, includes: controlling a first driving device 121 to draw a first liquid fluid from a first reservoir 200 and driving the drawn-out first liquid fluid to flow toward the chromatographic column 110, thereby propelling the first test solution prepared from at least a reference sample into the chromatographic column 110. In this embodiment, using a first liquid fluid with a low ion concentration as the medium for propelling the first test solution helps prevent the first liquid fluid from eluting the first test solution during the propelling process, thereby ensuring that the first test solution can be effectively adsorbed onto the chromatographic column 110.

[0113] In one implementation, after determining the target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly 120 and matching it with the chromatographic column 110, the controller is further configured to perform the following steps of the elution mode matching process: controlling the liquid phase fluid delivery assembly 120 to operate according to the target gradient elution mode to drive at least two elution phases of different ion concentrations made of at least two liquid phase fluids with different ion concentrations to flow sequentially through the chromatographic column 110, thereby performing gradient elution on a third test solution made of at least a reference and adsorbed within the chromatographic column 110, thereby forming a third analyte solution flowing out of the chromatographic column 110; controlling the detector 130 to detect the third analyte solution flowing out of the chromatographic column 110 and obtaining third detection information; and determining, based on the third detection information, whether the retention time of the first target component in the third analyte solution is within the target retention time range. In this implementation scheme, after determining the target gradient elution method based on the first detection information, the elution method matching process also needs to verify the target gradient elution method to ensure the consistency of the retention time of the first target component when eluting the sample solution adsorbed on the chromatographic column 110 using the target gradient elution method. Of course, the elution phase used in this target gradient elution method can also be prepared offline, which is similar to the offline preparation method of the elution phase described above, and will not be described in detail here.

[0114] In one embodiment, the above-described method of driving at least two different ion concentrations of eluent phases to flow sequentially through the chromatographic column 110 to perform gradient elution of a third test solution prepared from at least a reference and adsorbed within the chromatographic column 110 includes: driving at least two different ion concentrations of eluent phases prepared from at least two different liquid phase fluids to flow sequentially through the chromatographic column 110 to perform gradient elution of a third test solution prepared from at least a reference and adsorbed within the chromatographic column 110.

[0115] As one implementation method, determining whether the retention time of the first target component in the third test solution is within the target retention time range based on the third detection information includes: determining whether the retention time of each component in the third test solution is within the corresponding target retention time range based on the third detection information. In this implementation method, in the verification step of the elution mode matching process, in addition to verifying the retention time of the first target component, the retention times of other components are also verified, thus making the verification results more reliable. For example, based on the third detection information, determining whether the retention time of the first target component in the third test solution is within the first target retention time range, determining whether the retention time of the second component in the third test solution is within the corresponding second target retention time range, determining whether the retention time of the third component in the third test solution is within the corresponding third target retention time range, determining whether the retention time of the fourth component in the third test solution is within the corresponding fourth target retention time range, etc. Of course, in specific applications, as an alternative implementation method, in the verification step of the elution mode matching process, only the retention time of the first target component can be verified, or only the retention times of two components can be verified, or the retention times of three or more components can be verified.

[0116] In one implementation, the controller is also configured to perform an elution mode matching procedure before executing the calibration procedure. The calibration procedure primarily uses calibrators to calibrate the liquid chromatograph 100 to eliminate systematic errors in the liquid chromatograph 100, thereby ensuring the accuracy of sample detection results. In this embodiment, the elution mode matching procedure is executed before the calibration procedure, which helps to ensure the reliability of the calibration.

[0117] In one implementation, the controller is also configured to execute a calibration procedure if, based on third detection information, the retention time of the first target component in the third test solution is determined to be within the target retention time range.

[0118] In one implementation, the controller is also configured to output an alarm message if, based on the third detection information, it is determined that the retention time of the first target component in the third test liquid is not within the target retention time range, so as to remind the operator to intervene.

[0119] In one implementation, the controller is also configured to perform the following calibration procedure: controlling the liquid phase fluid delivery assembly 120 to operate according to a target gradient elution mode to drive at least two elution phases of different ion concentrations to flow sequentially through the chromatographic column 110, thereby performing gradient elution on a fourth test solution prepared from at least a calibrator and adsorbed within the chromatographic column 110, thus forming a fourth analyte solution eluting from the chromatographic column 110; controlling the detector 130 to detect the fourth analyte solution eluting from the chromatographic column 110 and obtaining fourth detection information; and obtaining and outputting the calibration result based on the fourth detection information. In this embodiment, the elution mode used in the calibration procedure is a target gradient elution mode determined by an elution mode matching procedure, which helps to prevent inaccurate calibration results due to mismatch between the chromatographic column 110 and the elution mode.

[0120] In one implementation, in the calibration procedure, driving at least two different ion concentrations of eluent phases to flow sequentially through the chromatographic column 110 includes: driving at least two different ion concentrations of eluent phases prepared from at least two different liquid phase fluids to flow sequentially through the chromatographic column 110. That is, the multiple different eluent phases used in the calibration procedure are prepared online using liquid phase fluid, and the principle is similar to that of online preparation of multiple different eluent phases in the sample detection procedure described above, and will not be detailed here. Of course, the multiple different eluent phases used in the calibration procedure can also be prepared offline, which is similar to the offline preparation method of the eluent phases described above, and will not be detailed here.

[0121] In one implementation, the controller is also configured to execute the elution mode matching process after receiving information that the characterization column 110 has been loaded or replaced. In this embodiment, when information that the characterization column 110 has been loaded or replaced is received, the controller automatically controls the execution of the elution mode matching process without requiring any operator intervention, thus making the execution of the elution mode matching process imperceptible to the operator. Of course, in specific applications, as an alternative implementation, the controller can also be configured to output information prompting the operator to execute the elution mode matching process upon receiving information that the characterization column 110 has been loaded or replaced. This information could, for example, be displayed as a pop-up window on the display screen 170 for the operator to select and confirm whether to execute the elution mode matching process. When the operator clicks the confirmation button on the display screen 170, the elution mode matching process is initiated.

[0122] In one implementation, the controller is further configured to execute an elution mode matching procedure after acquiring information indicating that the characterization column 110 has been loaded or replaced, and before executing the calibration procedure. In this embodiment, after the loading or replacement of the column 110 is completed, the elution mode matching procedure needs to be executed first to match the column 110 with the elution mode, ensuring the consistency of retention times for components eluted from the column 110; then the calibration procedure is executed to reduce errors in subsequent sample detection.

[0123] As one implementation, the controller is also configured to execute the sample testing procedure after the calibration procedure is completed. That is, sample testing is performed after calibration.

[0124] As one implementation, the controller is also configured to perform a quality control procedure after the calibration process is completed and before the sample testing process is performed. After the liquid chromatograph 100 is calibrated, quality control verification is required to ensure that the liquid chromatograph 100 can operate normally and stably.

[0125] In one implementation, the controller is also configured to, after acquiring information indicating that the characterization column 110 has been loaded or replaced, and before executing the sample detection procedure, sequentially execute the elution mode matching procedure, the calibration procedure, and the quality control procedure. In this way, the elution mode matching procedure ensures the compatibility between the chromatographic column 110 and the elution mode, while the calibration and quality control procedures significantly reduce errors in subsequent sample detection, ensuring the guiding value of the sample detection results for disease diagnosis and treatment.

[0126] In one implementation, the controller is also configured to perform the following quality control process: control the liquid phase fluid delivery assembly 120 to operate in a target gradient elution mode to drive at least two elution phases with different ion concentrations to flow through the chromatographic column 110, thereby performing gradient elution on a fifth test solution prepared from at least a quality control sample and adsorbed within the chromatographic column 110, thereby forming a fifth test solution flowing out of the chromatographic column 110; control the detector 130 to detect the fifth test solution flowing out of the chromatographic column 110 and obtain fifth detection information; and obtain and output the quality control result based on the fifth detection information.

[0127] In one embodiment, the chromatographic column 110 includes a first column having a first inner cavity and a first packing material filled in the first inner cavity.

[0128] In one implementation, both the reference sample and the sample contain a first target component. The aforementioned determination of the target gradient elution method for controlling the operation of the liquid chromatography fluid delivery assembly 120 and matching it with the chromatographic column 110, based on the first detection information, includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining the target gradient elution method based on the retention time of the first target component in the first test solution. In this embodiment, the target component corresponding to the retention time to be detected in the elution method matching process is a component also present in the sample. This facilitates the use of the target gradient elution method determined by the elution method matching process to execute the sample detection process, resulting in higher accuracy and reliability of the measured parameters of the second target component.

[0129] As one implementation method, the reference sample contains hemoglobin, and the sample also contains hemoglobin.

[0130] In one implementation method, the sample is bodily fluid from the human body, that is, bodily fluid collected from the human body. The liquid chromatograph 100 is an instrument used to perform liquid chromatographic analysis on bodily fluids from the human body.

[0131] In one implementation method, the sample is a blood sample, that is, a blood sample collected from a human body. The liquid chromatograph 100 is an instrument used to perform liquid chromatography analysis on human blood samples.

[0132] In one implementation, the reference material is at least one of a blood sample, a calibrator, and a quality control material. The blood sample is blood collected from a human body. The calibrator is an item used by the liquid chromatograph 100 to perform the calibration process. The quality control material is an item used by the liquid chromatograph 100 to perform the quality control process. Since the blood sample, calibrator, and quality control material are the three items used by the liquid chromatograph 100 and all have the same target components as the sample to be tested, using at least one of these as the reference material eliminates the need to add any new consumables to the liquid chromatograph 100. In other words, although the liquid chromatograph 100 has a new elution method matching process, the materials required for its operation do not increase.

[0133] In one implementation, determining the target gradient elution method for controlling the operation of the liquid phase fluid delivery assembly 120 and matching it with the chromatographic column 110 based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining the target gradient elution method based on the retention time of the first target component in the first test solution. The controller is also configured to perform the following detection steps in the sample detection process: controlling the detector 130 to detect the second test solution flowing out of the chromatographic column 110 and obtaining second detection information; and outputting a detection report containing at least the parameters of the second target component based on the second detection information. The first target component and the second target component are the same component. The parameters of the second target component are the main basis for determining whether the patient corresponding to the sample has a target disease in the chromatographic analysis project. In this implementation scheme, the first target component and the second target component are set to be the same component. That is, the target component corresponding to the retention time to be detected in the elution mode matching process and the target component corresponding to the parameter to be detected in the sample detection process report are the same component. In this way, the target gradient elution mode determined by the elution mode matching process is used to perform the sample detection process, and the measured parameters of the second target component are more accurate and reliable. Of course, in specific applications, as an alternative implementation scheme, the first target component and the second target component can also be different components.

[0134] Reference Figure 1 As shown, in one embodiment, the liquid chromatograph 100 further includes a sample processing component 150, a reversing valve 160, and a test solution preparation channel 140. A chromatographic column 110 is disposed between the reversing valve 160 and the detector 130. The sample processing component 150, the liquid transport component 120, the test solution preparation channel 140, and the chromatographic column 110 are respectively connected to different ports of the reversing valve 160 via liquid circuits. The reversing valve 160 is mainly used to switch the flow direction of the liquid in the liquid circuit. The sample processing component 150 is used to draw samples from the sample container and prepare the drawn samples into test solutions, and to deliver at least a portion of the test solution to the test solution preparation channel 140 via the reversing valve 160. The liquid phase fluid delivery assembly 120 is used to draw liquid phase fluid from the storage container and drive the liquid phase fluid to flow sequentially through the reversing valve 160 and the chromatographic column 110. This liquid phase fluid drives the test solution in the test solution preparation channel 140 to be delivered to the chromatographic column 110, and also drives the elution phase formed by the liquid phase fluid to elute the test solution adsorbed on the chromatographic column 110. The sample processing assembly 150 is a low-pressure assembly; the pressure output by the sample processing assembly 150 is insufficient to drive the test solution to flow through the chromatographic column 110. Therefore, the liquid phase fluid delivery assembly 120 is needed to drive the test solution to flow to the chromatographic column 110 using liquid phase fluid.

[0135] In one implementation, the reversing valve 160 has a switchable first connected state and a second connected state. In the first connected state, the reversing valve 160 connects the sample processing component 150 and the reagent preparation channel 140, and also connects the liquid phase fluid delivery component 120 and the chromatographic column 110. In the second connected state, the reversing valve 160 connects the liquid phase fluid delivery component 120, the reagent preparation channel 140, and the chromatographic column 110. The reversing valve 160 can switch between the first connected state and the second connected state under the control of a controller. In the first connected state, the sample processing component 150 can drive the reagent to be delivered to the reagent preparation channel 140, and the liquid phase fluid delivery component 120 can drive the liquid phase fluid to be delivered to the chromatographic column 110. In the second connected state, the liquid phase fluid delivery component can drive the liquid phase fluid to deliver the reagent in the reagent preparation channel 140 to the chromatographic column 110, and the sample processing component 150 cannot drive the reagent to be delivered to the reagent preparation channel 140.

[0136] In one implementation, the directional valve 160 is a six-way valve.

[0137] Reference Figure 1 As shown, in one embodiment, the sample processing assembly 150 includes a sampling component 151, a reaction container 152, and a sample infusion path 153. The sampling component 151 is used to draw a sample from the sample container and dispense it into the reaction container 152. The sample infusion path 153 is used to dispense hemolysis solution into the reaction container 152 and to deliver a test solution made from at least the sample and the hemolysis solution in the reaction container 152 to the test solution preparation channel 140 via a reversing valve 160. The sample infusion path 153 is provided with a suction and discharge power component 1531, which can be a combination of a syringe and a motor or a plunger pump and a motor. In this embodiment, the hemolysis solution is dispensed into the reaction container 152 by the sample infusion path 153, and the test solution made from the hemolysis solution and the sample is delivered from the reaction container 152 to the test solution preparation channel 140 by the sample infusion path 153. In this way, the sample infusion path 153 can be reused, and there is no need to set up an additional pipette and suction and discharge power component 1531 for dispensing the hemolysis solution. Of course, in specific applications, as an alternative implementation, the actions of dispensing the hemolysis treatment solution to the reaction vessel 152 and transporting the test solution to the test solution preparation channel 140 can also be driven by different suction and discharge power components 1531.

[0138] In one embodiment, the hemolysis solution includes at least one of the following liquids: a hemolytic agent containing a surfactant to dissolve and break down red blood cells in the sample; and deionized water, purified water, or distilled water that swells and breaks down red blood cells in the sample through osmotic pressure. The hemolytic agent containing a surfactant primarily dissolves red blood cells, causing them to release hemoglobin, thereby processing the blood sample (specifically, a whole blood sample) into a test solution. Deionized water, purified water, or distilled water primarily uses osmotic pressure to swell and break down red blood cells in the whole blood sample, causing them to release hemoglobin, thus also processing the whole blood sample into a test solution.

[0139] In one embodiment, when the hemolysis solution is a hemolysin containing a surfactant to dissolve and break down red blood cells in a sample, the infusion line 153 is used to draw the hemolysis solution from the hemolysin container 400 to the reaction container 152.

[0140] In one embodiment, the sampling component 151 includes a sample needle and a motion drive component for driving the movement of the sample needle.

[0141] Reference Figure 1 As shown, in one embodiment, the liquid chromatograph 100 further includes a first waste liquid channel 190. In a first connected state, a reversing valve 160 connects the sample processing assembly 150, the test solution preparation channel 140, and the first waste liquid channel 190, as well as the liquid phase fluid delivery assembly 120 and the chromatographic column 110. In a second connected state, the reversing valve 160 connects the liquid phase fluid delivery assembly 120, the test solution preparation channel 140, and the chromatographic column 110, as well as the sample processing assembly 150 and the first waste liquid channel 190.

[0142] In one implementation, the liquid chromatograph 100 also includes a second waste liquid channel, to which the detector 130 is connected. The first waste liquid channel 190 and the second waste liquid channel can be the same waste liquid channel or two independent waste liquid channels.

[0143] Reference Figure 1 and Figure 2 As shown, in one embodiment, the liquid chromatograph 100 also includes a housing 180 and a display screen 170. The display screen 170 is located on one side of the housing 180 of the liquid chromatograph 100 for the operator to view chromatographic analysis information of the sample. The chromatographic column 110, the liquid flow delivery assembly 120, the detector 130, and the controller are all installed inside the housing 180.

[0144] As one implementation method, a specific implementation of the elution mode matching process is as follows: The sampling component 151 is controlled to draw reference sample from the reference sample container and distribute it to the reaction container 152, so that the reference sample reacts with the hemolysis solution in the reaction container 152 to prepare a first test solution. The sample delivery path 153 is controlled to transport the first test solution from the reaction container 152 to the test solution preparation channel 140. The first driving component 121 is controlled to operate according to a preset sample pushing mode to drive the first liquid phase fluid to flow sequentially into the test solution preparation channel 140 and the chromatographic column 110, thereby pushing the first test solution in the test solution preparation channel 140 into the chromatographic column. The column 110 is controlled to operate the first driving device 121 and the second driving device 122 according to a preset elution mode, so as to drive the elution phase made of the first liquid phase fluid or the first liquid phase fluid and the second liquid phase fluid to flow through the chromatographic column 110, thereby eluting the first test solution adsorbed in the chromatographic column 110, and forming the first test solution flowing out of the chromatographic column 110; the detector 130 is controlled to detect the first test solution flowing out of the chromatographic column 110 and obtain the first detection information; based on the first detection information, the retention time of the first target component in the first test solution is obtained, and based on the first target component in the first test solution... The retention time is determined to establish a target gradient elution mode that matches the chromatographic column 110; the sampling component 151 is controlled to draw reference sample from the reference sample container and distribute it to the reaction container 152, so that the reference sample reacts with the hemolysis solution in the reaction container 152 to prepare the third test solution; the sample delivery path 153 is controlled to deliver the third test solution from the reaction container 152 to the test solution preparation channel 140; the first driving component 121 is controlled to operate according to a preset sample pushing mode to drive the first liquid phase fluid to flow sequentially into the test solution preparation channel 140 and the chromatographic column 110, thereby pushing the third test solution in the test solution preparation channel 140 into the chromatographic column 110. 10; Control the first driving device 121 and the second driving device 122 to operate in a target gradient elution mode to drive at least two elution phases made of the first liquid phase fluid and the second liquid phase fluid to flow sequentially through the chromatographic column 110, thereby performing gradient elution on the first test solution adsorbed in the chromatographic column 110, and thus forming a third test solution flowing out of the chromatographic column 110; Control the detector 130 to detect the third test solution flowing out of the chromatographic column 110 and obtain third detection information; Based on the third detection information, determine whether the retention time of each component in the third test solution is within the corresponding target retention time range.

[0145] As one implementation method, a specific implementation of the sample detection process is as follows: The sampling component 151 is controlled to draw a sample from the sample container and distribute it to the reaction container 152, so that the sample reacts with the hemolysis solution in the reaction container 152 to prepare a second test solution. The sample delivery path 153 is controlled to transport the second test solution from the reaction container 152 to the test solution preparation channel 140. The first driving component 121 is controlled to operate according to a preset sample pushing mode to drive the first liquid phase fluid to flow sequentially into the test solution preparation channel 140 and the chromatographic column 110, thereby pushing the sample in the test solution preparation channel 140... The second test solution flows into the chromatographic column 110; the first driving device 121 and the second driving device 122 are controlled to operate in a target gradient elution mode to drive at least two elution phases made of the first liquid phase fluid and the second liquid phase fluid to flow sequentially through the chromatographic column 110, thereby performing gradient elution on the second test solution adsorbed in the chromatographic column 110, and thus forming the second test solution flowing out of the chromatographic column 110; the detector 130 is controlled to detect the second test solution flowing out of the chromatographic column 110 and obtain the second detection information; based on the second detection information, the detection result of the sample is output.

[0146] In one implementation, the liquid chromatograph 100 is a glycated hemoglobin analyzer. The controller is also configured to perform the following detection steps in the sample detection process: control the detector 130 to detect the second analyte flowing from the chromatographic column 110 and obtain second detection information; based on the second detection information, output a detection report containing at least the parameters of the second target component. The second target component is HbA1c. HbA1c is glycated hemoglobin A1c, a product formed by the non-enzymatic reaction between hemoglobin in red blood cells and glucose in the blood. The level of HbA1c is an important indicator for the diagnosis of diabetes. As glycated protein analyzers gradually develop towards faster detection speeds and higher resolution, high-speed glycated protein analyzers have stricter requirements for retention time consistency than low-speed glycated protein analyzers to ensure the reliability of chromatographic peak separation and identification. The glycated hemoglobin analyzer of this implementation scheme adopts the above-mentioned control scheme, that is, firstly, the target gradient elution mode matching the chromatographic column 110 is determined by matching the elution mode process, and then the sample detection process is executed using the target gradient elution mode. This ensures the consistency of the retention time of the target components detected by different chromatographic columns 110, fully guarantees the reliability of chromatographic peak separation and identification of the glycated protein analyzer, and is conducive to the accelerated development of glycated protein analyzers.

[0147] In one implementation, both the first target component and the second target component are HbA1c. Of course, in specific applications, the arrangement of the first and second target components is not limited to this. For example, as an alternative implementation, the second target component may be HbA1c, and the first target component may be HbA0, HbA1a, or HbA1b, etc.

[0148] A first aspect of this invention also provides a control method for a liquid chromatograph 100. This control method includes the following steps in an elution mode matching process: controlling a liquid phase fluid delivery assembly 120 to operate according to a preset elution mode, driving at least one elution phase through a chromatographic column 110 to elute a first test solution prepared from at least a reference and adsorbed within the chromatographic column 110, thereby forming a first analyte effluent from the chromatographic column 110; controlling a detector 130 to detect the first analyte effluent from the chromatographic column 110 and obtaining first detection information; and determining a target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly 120 and matching it with the chromatographic column 110 based on the first detection information. The control method also includes the following elution step in a sample detection process: controlling the liquid phase fluid delivery assembly 120 to operate according to a target gradient elution mode, driving at least two elution phases of different ion concentrations to sequentially flow through the chromatographic column 110, thereby performing gradient elution on a second test solution prepared from at least a sample and adsorbed within the chromatographic column 110, thereby forming a second analyte effluent from the chromatographic column 110.

[0149] As one implementation method, the target gradient elution mode in the above control method includes one of the following control modes: controlling the liquid phase fluid delivery component 120 to drive at least two liquid phase fluids with different ion concentrations to prepare and deliver at least two different ion concentrations of elution phase to the chromatographic column 110 at different time periods according to different sub-target concentrations; controlling the liquid phase fluid delivery component 120 to drive at least two liquid phase fluids with different ion concentrations to prepare and deliver at least two different ion concentrations of elution phase to the chromatographic column 110 at different time periods according to different sub-target ratio parameters; controlling the liquid phase fluid delivery component 120 to drive at least two liquid phase fluids with different ion concentrations to prepare and deliver at least two different ion concentrations of elution phase according to the target gradient elution curve to the chromatographic column 110.

[0150] As one implementation, in the above control method, determining the target gradient elution mode for controlling the operation of the liquid phase fluid delivery component 120 and matching the chromatographic column 110 based on the first detection information includes: determining one of a plurality of preset gradient elution modes as the target gradient elution mode based on the first detection information.

[0151] As one implementation, in the above control method, determining one of a plurality of preset gradient elution methods as the target gradient elution method based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining one of a plurality of preset gradient elution methods corresponding to the retention time of the first target component in the first test solution as the target gradient elution method based on the retention time of the first target component in the first test solution.

[0152] Other parts and principles of the control method for the liquid chromatograph 100 provided in the first aspect of the present invention can be referred to the control scheme and principle of the controller described in the above liquid chromatograph 100, and will not be described in detail here.

[0153] A first aspect of this invention also provides a computer-readable storage medium storing a computer program. When the computer program is executed by a processor (e.g., the controller described above), it causes the processor to implement the steps of the control method for the liquid chromatograph 100 described above. The computer-readable storage medium may be an internal storage unit of the liquid chromatograph 100, such as a hard disk or memory of the liquid chromatograph 100; or, it may be an external storage device of the liquid chromatograph 100, such as a pluggable hard disk, smart media card (SMC), secure digital card (SD), flash card, etc., equipped on the liquid chromatograph 100.

[0154] The liquid chromatograph 100 and its control method and computer-readable storage medium provided in the first aspect of the present invention address the problem of the retention time deviation of components by different batches of chromatographic columns 110 and the matching of elution phase and elution scheme. The present invention proposes a scheme in which, after changing the chromatographic column 110, a target gradient elution scheme matching the chromatographic column 110 is first determined through an elution scheme matching process, and then the calibration process and sample detection process are performed using the target gradient elution scheme.

[0155] The elution mode matching process includes the following specific steps: After replacing the column 110 in the liquid chromatograph 100 and before starting the calibration process, the system automatically injects a random blood sample or a reference such as a glycated hemoglobin calibrator or quality control sample. The first test solution prepared from the reference is then delivered to the column 110. Isocratic elution is performed using the first liquid phase fluid, or gradient elution is performed using the first liquid phase fluid and the second liquid phase fluid. The retention time of HbA1c after replacing the column 110 is detected. Based on this retention time, the system automatically determines the target gradient elution mode. Then, the system automatically injects the reference sample and delivers the third test solution prepared from the reference sample to the column 110. The first liquid phase fluid and the second liquid phase fluid are delivered according to the target gradient elution mode to perform gradient elution on the third test solution adsorbed on the column 110. The system then determines whether the chromatographic peaks of HbA1a, ..., HbA1c, HbA0, etc., are within the recognition range of the algorithm.

[0156] A second aspect of the present invention provides a liquid chromatograph 100, which includes a chromatographic column 110, a liquid fluid delivery assembly 120, a detector 130, and a controller. The controller is configured to: after acquiring information indicating that the chromatographic column 110 has been loaded or replaced, and before performing a calibration procedure, execute an elution mode matching procedure to determine a target gradient elution mode for controlling the operation of the liquid fluid delivery assembly 120 and matching the chromatographic column 110; the controller is also configured to perform the following elution step in a sample detection procedure: control the liquid fluid delivery assembly 120 to operate according to the target gradient elution mode to drive at least two elution phases of different ion concentrations to flow sequentially through the chromatographic column 110, thereby performing gradient elution on a second test solution prepared from at least a sample and adsorbed within the chromatographic column 110, thereby forming a second analyte solution flowing out of the chromatographic column 110. The liquid chromatograph 100 provided in the second aspect of the present invention performs an elution mode matching process after the chromatographic column 110 is loaded or replaced, and before the calibration process is executed, in order to determine the target gradient elution mode that matches the chromatographic column 110; thereby, it can effectively solve the technical problem that the sample detection results are affected by the mismatch between the chromatographic column 110 and the elution mode.

[0157] A second aspect of the present invention also provides a control method for a liquid chromatograph 100, the control method comprising: after obtaining information that the characterization column 110 has been loaded or replaced, and before performing a calibration procedure, performing an elution mode matching procedure to determine a target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly 120 and matching the chromatographic column 110; controlling the liquid phase fluid delivery assembly 120 to operate according to the target gradient elution mode to drive at least two elution phases of different ion concentrations to flow sequentially through the chromatographic column 110, thereby performing gradient elution on a second test solution prepared from at least a sample and adsorbed within the chromatographic column 110, thereby forming a second analyte solution flowing out of the chromatographic column 110.

[0158] As one implementation, the above-described elution mode matching process for determining a target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly 120 and matching it with the chromatographic column 110 includes: performing the elution mode matching process to obtain the retention time of a first target component, and determining a target gradient elution mode based on the retention time of the first target component.

[0159] As one implementation method, the target gradient elution method includes one of the following control methods: controlling the liquid phase fluid delivery assembly 120 to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different reservoir, to prepare at least two different ion concentrations of eluent at different time periods and to deliver at least two different ion concentrations of eluent to the chromatographic column 110; controlling the liquid phase fluid delivery assembly 120 to drive at least two different ion concentrations of liquid phase fluids, each loaded in a different reservoir, to prepare at different time periods according to different sub-target ratio parameters and to deliver at least two different ion concentrations of eluent to the chromatographic column 110; controlling the liquid phase fluid delivery assembly 120 to drive at least two different ion concentrations of liquid phase fluids, each loaded in a different reservoir, to prepare at least two different ion concentrations of eluent according to a target gradient elution curve and to deliver at least two different ion concentrations of eluent to the chromatographic column 110; controlling the liquid phase fluid delivery assembly 120 to drive at least two different ion concentrations of eluent, each loaded in a different reservoir, to deliver one of the at least two eluent phases to the chromatographic column 110 at different time periods.

[0160] As one implementation method, different sub-target scale parameters are different sub-target volume scale parameters.

[0161] A second aspect of the present invention also provides a computer-readable storage medium storing a computer program that, when executed by a processor (e.g., the controller described above), causes the processor to implement the steps of the control method for the liquid chromatograph 100 described above.

[0162] Other parts and principles of the liquid chromatograph 100 and its control method and computer-readable storage medium provided in the second aspect of the present invention can be referred to the liquid chromatograph 100 and its control method and computer-readable storage medium provided in the first aspect above, and will not be described in detail here.

[0163] A third aspect of the present invention provides a liquid chromatograph 100, which includes a chromatographic column 110, a liquid phase fluid delivery assembly 120, a detector 130, and a controller. The controller is configured to perform an elution mode matching process as follows: acquiring the retention time of a first target component associated with the chromatographic column 110, and determining a target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly 120 and matching the chromatographic column 110 based on the retention time of the first target component. The controller is also configured to perform the following elution step in a sample detection process: controlling the liquid phase fluid delivery assembly 120 to operate according to the target gradient elution mode to drive at least two elution phases of different ion concentrations to flow sequentially through the chromatographic column 110, thereby performing gradient elution on a second test solution prepared from at least a sample and adsorbed within the chromatographic column 110, thereby forming a second analyte solution flowing out of the chromatographic column 110. The liquid chromatograph 100 provided in the third aspect of the present invention determines the target gradient elution mode that matches the chromatographic column 110 by measuring the retention time of the first target component associated with the chromatographic column 110, thereby solving the technical problem that the sample detection results are affected due to the mismatch between the chromatographic column 110 and the elution mode.

[0164] A third aspect of the present invention also provides a control method for a liquid chromatograph 100, the control method comprising the following steps in an elution mode matching process: obtaining the retention time of a first target component associated with a chromatographic column 110, and determining a target gradient elution mode for controlling the operation of a liquid phase fluid delivery assembly 120 and matching it with the chromatographic column 110 based on the retention time of the first target component. The control method further comprises the following elution step in a sample detection process: controlling the liquid phase fluid delivery assembly 120 to operate according to the target gradient elution mode to drive at least two elution phases of different ion concentrations to sequentially flow through the chromatographic column 110, thereby performing gradient elution on a second test solution prepared from at least a sample and adsorbed within the chromatographic column 110, thereby forming a second analyte solution eluting from the chromatographic column 110.

[0165] As one embodiment for obtaining the retention time of a first target component associated with the chromatographic column 110, the liquid chromatograph 100 further includes an identification component for identifying an identification code on the chromatographic column 110. Obtaining the retention time of the first target component associated with the chromatographic column 110 includes: obtaining the retention time of the first target component based on information fed back from the identification code on the chromatographic column 110 identified by the identification component. The identification code can be a barcode, a QR code, or an RFID code (radio frequency code). In this implementation scheme, before the chromatographic column 110 leaves the factory, an elution mode matching process is performed in advance to obtain the retention time of the first target component associated with the chromatographic column 110, and the retention time is bound to the identification code on the chromatographic column 110. In this way, when loading or replacing the chromatographic column 110, the identification code on the chromatographic column 110 can be directly identified by the identification component to obtain the retention time of the first target component associated with the chromatographic column 110. This eliminates the need for the liquid chromatograph 100 at the client end to perform elution detection on the reference sample to obtain the retention time of the first target component, thereby improving the operating efficiency of the liquid chromatograph 100.

[0166] Alternatively, as another implementation for obtaining the retention time of the first target component associated with the chromatographic column 110, the liquid chromatograph 100 further includes a human-machine interface (HMI). Obtaining the retention time of the first target component associated with the chromatographic column 110 includes: obtaining the retention time of the first target component associated with the chromatographic column 110 based on information input by the operator through the HMI. The HMI includes at least one of a display screen 170, a keyboard, a mouse, and a microphone. In this embodiment, the retention time of the first target component associated with the chromatographic column 110 can be recorded in the manufacturer's instructions for the chromatographic column 110, and input by the operator through the HMI according to the manufacturer's instructions for the chromatographic column 110.

[0167] In one implementation, the human-machine interface includes a display screen 170. The controller is also configured to, upon receiving information indicating that the chromatographic column 110 has been loaded or replaced, control the display screen 170 to display an information input interface for the operator to input the retention time of the first target component associated with the chromatographic column 110.

[0168] Of course, in specific applications, as an alternative implementation, the retention time of the first target component associated with the chromatographic column 110 can also be obtained by performing a matching process of elution mode in the liquid chromatograph 100 provided in the first aspect above.

[0169] A third aspect of the present invention also provides a computer-readable storage medium storing a computer program, which, when executed by a processor (such as the controller described above), causes the processor to implement the steps of the control method for the liquid chromatograph 100 described above.

[0170] Other parts and principles of the liquid chromatograph 100 and its control method and computer-readable storage medium provided in the third aspect of the present invention can be referred to the liquid chromatograph 100 and its control method and computer-readable storage medium provided in the first and second aspects above, and will not be described in detail here.

[0171] The above description is merely a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made using the contents of the present invention's specification and drawings under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A liquid chromatograph, characterized in that: Includes chromatographic columns, liquid phase fluid delivery components, detectors, and controllers; The controller is configured to execute the following elution mode matching process: controlling the liquid phase fluid delivery assembly to operate according to a preset elution mode to drive at least one elution phase through the chromatographic column, thereby eluting a first test solution prepared from at least a reference and adsorbed within the chromatographic column, thus forming a first analyte solution eluting from the chromatographic column; controlling the detector to detect the first analyte solution eluting from the chromatographic column and obtaining first detection information; and determining a target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly and matching it with the chromatographic column based on the first detection information. The controller is also configured to perform the following elution step in the sample detection process: control the liquid phase fluid delivery assembly to operate in the target gradient elution mode to drive at least two elution phases with different ion concentrations to flow sequentially through the chromatographic column, thereby performing gradient elution on a second test solution prepared from at least the sample and adsorbed within the chromatographic column, thereby forming a second test solution flowing out of the chromatographic column.

2. The liquid chromatograph as described in claim 1, characterized in that: The target gradient elution method includes one of the following control methods: The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared at different sub-target concentrations at different time periods and delivered to the chromatographic column at at least two different ion concentrations; The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared at different time periods according to different sub-target ratio parameters and delivered to the chromatographic column. The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared according to a target gradient elution curve and delivered to the chromatographic column. The liquid-phase fluid delivery assembly is controlled to drive at least two elution phases with different ion concentrations, each loaded in a different storage container, to deliver one of the at least two elution phases to the chromatographic column at different time periods; Preferably, the different sub-target ratio parameters are different sub-target volume ratios.

3. The liquid chromatograph as described in claim 1 or 2, characterized in that: The liquid chromatograph has multiple preset gradient elution modes stored in its memory. The step of determining the target gradient elution mode for controlling the operation of the liquid phase fluid delivery component and matching the chromatographic column based on the first detection information includes: determining one of the plurality of preset gradient elution modes as the target gradient elution mode based on the first detection information.

4. The liquid chromatograph as described in claim 3, characterized in that: The step of determining one of the plurality of preset gradient elution methods as the target gradient elution method based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining one of the plurality of preset gradient elution methods corresponding to the retention time of the first target component in the first test solution as the target gradient elution method based on the retention time of the first target component.

5. The liquid chromatograph as described in claim 1 or 2, characterized in that: The liquid chromatograph has multiple preset gradient elution modes stored in its memory. The step of determining, based on the first detection information, a target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly and matching the chromatographic column includes: Based on the first detection information, the actual retention time of the first target component in the first test solution is obtained, and the actual retention time is compared with the target retention time range. If the actual retention time is less than the lower limit of the target retention time range, then one of the pre-stored gradient elution methods in which the ion concentration of the elution phase used to elute the first target component during the elution process is less than the ion concentration of the elution phase used to elute the first target component during the elution process of the preset elution method is determined as the target gradient elution method. If the actual retention time is greater than the upper limit of the target retention time range, then one of the pre-stored gradient elution methods in which the ion concentration of the elution phase used to elute the first target component during the elution process is greater than the ion concentration of the elution phase used to elute the first target component during the elution process of the preset elution method is determined as the target gradient elution method.

6. The liquid chromatograph as described in claim 1 or 2, characterized in that: The step of determining the target gradient elution mode for controlling the operation of the liquid phase fluid delivery component and matching the chromatographic column based on the first detection information includes: obtaining elution correlation information for characterizing the interaction strength between the first target component in the first test solution and the chromatographic column and the elution phase based on the first detection information; and determining the target gradient elution mode based on the elution correlation information; wherein the elution correlation information includes at least one of the following: the retention time of the first target component in the first test solution, and the chromatographic peak position of the first target component in the chromatographic curve of the first test solution; And / or, determining the target gradient elution mode for controlling the operation of the liquid phase fluid delivery component and matching the chromatographic column based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining the target gradient elution mode based on the retention time of the first target component in the first test solution.

7. The liquid chromatograph as described in claim 1, characterized in that: The reference sample and the sample both contain a first target component; determining the target gradient elution method for controlling the operation of the liquid phase fluid delivery assembly and matching the chromatographic column based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining the target gradient elution method based on the retention time of the first target component in the first test solution. And / or, the components of the reference sample contain hemoglobin; And / or, the reference material is at least one of blood sample, calibrator, and quality control material.

8. The liquid chromatograph as described in claim 1, 2, or 7, characterized in that: The sample is a blood sample; the controller is also configured to perform the following detection steps in the sample detection process: control the detector to detect the second test solution flowing out of the chromatographic column and obtain second detection information; based on the second detection information, output a detection report containing at least a second target component parameter, wherein the second target component is HbA1c.

9. The liquid chromatograph as described in claim 1, 2, or 7, characterized in that: The step of determining the target gradient elution mode for controlling the operation of the liquid phase fluid delivery component and matching the chromatographic column based on the first detection information includes: obtaining the retention time of the first target component in the first test solution based on the first detection information, and determining the target gradient elution mode based on the retention time of the first target component in the first test solution; The controller is also configured to perform the following detection steps in the sample detection process: control the detector to detect the second test solution flowing out of the chromatographic column and obtain second detection information; and output a detection report containing at least the second target component parameter based on the second detection information. Wherein, the first target component and the second target component are the same component or are different components.

10. The liquid chromatograph as described in claim 1, characterized in that: The preset elution method includes one of the following control methods: The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations and loaded in different storage containers to prepare and deliver at least two different preset ion concentrations of elution phase to the chromatographic column at different time periods, so as to perform gradient elution of the first test solution adsorbed in the chromatographic column; The liquid phase fluid delivery assembly is controlled to drive at least two elution phases with different ion concentrations and loaded in different storage containers to deliver one of the at least two elution phases to the chromatographic column at different time periods, so as to perform gradient elution of the first test solution adsorbed in the chromatographic column; The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations and respectively loaded in different storage containers to mix and deliver the elution phase of a single concentration to the chromatographic column, so as to perform isocratic elution of the first test solution adsorbed in the chromatographic column; The liquid phase fluid delivery assembly is controlled to drive the elution phase, formed by a liquid phase fluid loaded in a storage container, to be delivered to the chromatographic column to perform isocratic elution of the first test solution adsorbed in the chromatographic column.

11. The liquid chromatograph as described in claim 1, 2, 7, or 10, characterized in that: The liquid phase fluid delivery assembly includes a first driving device and a second driving device. The first driving device is used to draw a first liquid phase fluid from a first liquid storage container and drive the drawn first liquid phase fluid to be delivered through the chromatographic column. The second driving device is used to draw a second liquid phase fluid with an ion concentration greater than that of the first liquid phase ion concentration from the second storage container, and drive the drawn second liquid phase fluid to flow through the chromatographic column; The preset elution method includes one of the following control methods: The first driving device and the second driving device are controlled to operate according to the preset elution mode, so as to drive the first liquid phase fluid and the second liquid phase fluid to prepare and deliver at least two different preset ion concentrations of elution phase to the chromatographic column at different time periods, so as to perform gradient elution of the first test solution adsorbed in the chromatographic column; The first driving device and the second driving device are controlled to operate according to the preset elution mode, so as to drive the first liquid phase fluid and the second liquid phase fluid to mix and deliver a single concentration of elution phase to the chromatographic column, so as to perform isocratic elution of the first test solution adsorbed in the chromatographic column; The first driving device is individually controlled to drive the first liquid phase fluid to flow into the chromatographic column, so as to perform isocratic elution of the first test solution adsorbed in the chromatographic column.

12. The liquid chromatograph as described in claim 1, 2, 7, or 10, characterized in that: Before controlling the liquid phase fluid delivery assembly to operate according to a preset elution mode, the controller is also configured to perform the following steps of the elution mode matching process: controlling the liquid phase fluid delivery assembly to operate according to a preset push mode to drive at least one of the elution phases to flow toward the chromatographic column, thereby pushing the first test solution, made at least from a reference, into the chromatographic column.

13. The liquid chromatograph as described in claim 1, 2, 7, or 10, characterized in that: After determining the target gradient elution mode for controlling the operation of the liquid phase fluid delivery assembly and matching the chromatographic column, the controller is further configured to perform the following steps of the elution mode matching process: The liquid phase fluid delivery assembly is controlled to operate according to the target gradient elution mode to drive at least two different ion concentrations of the elution phase to flow sequentially through the chromatographic column, thereby performing gradient elution on a third test solution prepared at least from a reference and adsorbed within the chromatographic column, thereby forming a third analyte solution flowing out of the chromatographic column; The detector is controlled to detect the third test solution flowing out of the chromatographic column and to obtain third detection information; Based on the third detection information, it is determined whether the retention time of the first target component in the third test solution is within the target retention time range.

14. The liquid chromatograph as described in claim 1, 2, 7, or 10, characterized in that: The controller is also configured to execute the elution mode matching process before executing the calibration process; And / or, the controller is further configured to perform the following calibration procedure: control the liquid phase fluid delivery assembly to operate according to the target gradient elution mode to drive at least two different ion concentrations of the elution phase to flow sequentially through the chromatographic column, thereby performing gradient elution on the fourth test solution, which is at least made of calibrator and adsorbed in the chromatographic column, to form a fourth test solution flowing out of the chromatographic column; control the detector to detect the fourth test solution flowing out of the chromatographic column and obtain fourth detection information; and obtain and output the calibration result based on the fourth detection information.

15. The liquid chromatograph as described in claim 14, characterized in that: The controller is also configured to execute the elution mode matching process after acquiring information indicating that the column loading or replacement is complete, and before executing the calibration process. And / or, the controller is further configured to execute the sample detection process after the calibration process is completed.

16. The liquid chromatograph as described in claim 1, 2, 7, or 10, characterized in that: The controller is also configured to: when it receives information indicating that the column loading or replacement is complete, output information to prompt the execution of the elution mode matching process.

17. A liquid chromatograph, characterized in that: Includes chromatographic columns, liquid phase fluid delivery components, detectors, and controllers; The controller is configured to: after acquiring information indicating that the column loading or replacement is complete, and before executing the calibration procedure, execute an elution mode matching procedure to determine a target gradient elution mode that controls the operation of the liquid phase fluid delivery assembly and matches the column. The controller is also configured to perform the following elution step in the sample detection process: control the liquid phase fluid delivery assembly to operate in the target gradient elution mode to drive at least two elution phases with different ion concentrations to flow sequentially through the chromatographic column, thereby performing gradient elution on a second test solution prepared from at least the sample and adsorbed within the chromatographic column, thereby forming a second test solution flowing out of the chromatographic column.

18. The liquid chromatograph as described in claim 17, characterized in that: The execution of the elution mode matching process to determine the target gradient elution mode for controlling the operation of the liquid phase fluid delivery component and matching the chromatographic column includes: executing the elution mode matching process to obtain the retention time of the first target component, and determining the target gradient elution mode based on the retention time of the first target component; And / or, the target gradient elution method includes one of the following control methods: The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared at different sub-target concentrations at different time periods and delivered to the chromatographic column at at least two different ion concentrations; The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared at different time periods according to different sub-target ratio parameters and delivered to the chromatographic column. The liquid phase fluid delivery assembly is controlled to drive at least two liquid phase fluids with different ion concentrations, each loaded in a different storage container, to be prepared according to a target gradient elution curve and delivered to the chromatographic column. The liquid-phase fluid delivery assembly is controlled to drive at least two elution phases with different ion concentrations, each loaded in a different storage container, to deliver one of the at least two elution phases to the chromatographic column at different time periods; Preferably, the different sub-target ratio parameters are different sub-target volume ratios.

19. A liquid chromatograph, characterized in that: Includes chromatographic columns, liquid phase fluid delivery components, detectors, and controllers; The controller is configured to perform the following elution mode matching process: obtain the retention time of a first target component associated with the chromatographic column, and determine a target gradient elution mode that is matched with the chromatographic column and is used to control the operation of the liquid phase fluid delivery assembly based on the retention time of the first target component; The controller is also configured to perform the following elution step in the sample detection process: control the liquid phase fluid delivery assembly to operate in the target gradient elution mode to drive at least two elution phases with different ion concentrations to flow sequentially through the chromatographic column, thereby performing gradient elution on a second test solution prepared from at least the sample and adsorbed within the chromatographic column, thereby forming a second test solution flowing out of the chromatographic column.

20. The liquid chromatograph as described in claim 19, characterized in that: The liquid chromatograph further includes an identification component for identifying the identification code on the chromatographic column; obtaining the retention time of the first target component associated with the chromatographic column includes: obtaining the retention time of the first target component based on the information fed back by the identification code on the chromatographic column identified by the identification component; Alternatively, the liquid chromatograph may further include a human-machine interface component, wherein obtaining the retention time of the first target component associated with the chromatographic column includes: obtaining the retention time of the first target component based on information input by the operator through the human-machine interface component.

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