Data storage medium, data circulating device and application thereof

Abstract

The application provides a data storage medium, a data circulation device and application thereof. A first aspect of the application provides a data storage medium, comprising a nucleic acid molecule aerosol, raw materials of the nucleic acid molecule aerosol comprising water-in-oil particles, the water-in-oil particles comprising an oil phase located at an outer layer and a water phase located at an inner layer, the water phase comprising a nucleic acid molecule encapsulated by an organic framework material, and a base sequence of the nucleic acid molecule encoding data information. The application first extends the nucleic acid molecule data storage to the air medium through the aerosol mode, realizes a real carrier-free distributed storage, and the aerosol has good suspensibility, the nucleic acid molecule data in the particles has a long storage time, and the data loading / access speed is fast.

Description

Technical Field

[0001] This application relates to the field of data storage technology, and in particular to data storage devices and their applications. Background Technology

[0002] With the rapid development of the digital society, data storage technology has become the core of information infrastructure. Traditional silicon-based electronic storage is gradually approaching its physical limits, and energy consumption and cost are rising exponentially. DNA, with its extremely high storage density and chemical stability, has become an important direction for breaking through the bottleneck of silicon-based storage. However, most current DNA storage systems adopt the following modes: (1) liquid phase system, which dissolves DNA-encoded information in a solution and reads and writes data through PCR, sequencing and other methods; (2) solid phase system: DNA is adsorbed or embedded in solid materials (such as resin, microspheres, and thin films) to enhance stability. These systems all rely on physical containers for carrying data, and data migration needs to rely on mechanical or liquid flow to achieve free propagation in the air. For example, a DNA magnetic tape data storage device based on a solid substrate carries DNA files through physical compartments to achieve automated writing and reading, but it is still limited to a closed solid phase structure and cannot escape the physical space to achieve data suspension, diffusion and spontaneous propagation in space. Therefore, it is necessary to provide a nano aerosol particle that can stably suspend and store data in the air and can achieve automatic release, circulation and recycling. Summary of the Invention

[0003] The main objective of this invention is to provide a data storage medium, a data circulation device, and their applications.

[0004] To achieve the above objectives, a first aspect of the present invention provides a data storage medium comprising a nucleic acid molecular aerosol, wherein the raw material of the nucleic acid molecular aerosol comprises water-in-oil particles, wherein the water-in-oil particles comprise an outer oil phase and an inner aqueous phase, the aqueous phase comprising nucleic acid molecules encapsulated by an organic framework material, and the base sequence of the nucleic acid molecules encoding data information.

[0005] In some implementations, the particle size of the nucleic acid molecular aerosol is 10~500 nm.

[0006] In some embodiments, the organic framework material includes at least one of metal-organic framework materials, covalent organic framework materials, and hydrogen-bonded organic framework materials.

[0007] In some embodiments, the metal-organic framework material includes at least one of HKUST-1, ZIF-8, ZIF-67, and UIO-66.

[0008] In some embodiments, the oil phase includes at least one of vegetable oil, mineral oil, and silicone oil.

[0009] In some embodiments, the interface between the oil phase and the aqueous phase also includes an ionic polymer material.

[0010] In some embodiments, the ionic polymeric material includes at least one of polyethyleneimine, polydiallyldimethylammonium chloride, polyacrylic acid, and sodium polystyrene sulfonate.

[0011] In some embodiments, the mass-volume percentage of the ionic polymeric material to the oil phase is 0.1 to 50% w / v.

[0012] In some embodiments, the interface between the oil phase and the aqueous phase also includes a surfactant.

[0013] In some embodiments, the surfactant is present in a mass-volume percentage of 0.01 to 5% w / v in the oil phase.

[0014] A second aspect of the present invention provides a method for generating the aforementioned data storage medium, comprising the following steps: A mixed solution containing oil and water phases is formed into a dispersion of water-in-oil particles, which is then generated into nucleic acid molecular aerosols by ultrasonic atomization.

[0015] In some implementations, the ultrasonic frequency of ultrasonic atomization is 1~10 MHz.

[0016] In some implementations, the pore size of the ultrasonically atomized microporous atomizing sheet is 1~10 μm.

[0017] A third aspect of the present invention provides a nucleic acid molecular aerosol recovery unit, comprising: The first power element is used to recover gas containing nucleic acid molecular aerosol from the outside. The raw material of the nucleic acid molecular aerosol includes water-in-oil particles, which include an outer oil phase and an inner water phase. The water phase includes nucleic acid molecules encapsulated by an organic framework material. The base sequence of the nucleic acid molecules encodes data information. A condenser is used to liquefy a gas to obtain a solution containing water-in-oil particles.

[0018] In some embodiments, the nucleic acid molecular aerosol recovery unit further includes a storage tank connected to a condenser for collecting a solution containing water-in-oil particles.

[0019] In some implementations, the first power element includes a fan.

[0020] A fourth aspect of the present invention provides an apparatus comprising the aforementioned nucleic acid molecular aerosol recovery unit.

[0021] A fifth aspect of the present invention provides a data recycling apparatus, comprising the aforementioned nucleic acid molecular aerosol recovery unit and: The nucleic acid molecular aerosol generation unit includes an ultrasonic atomization component for forming a dispersion of water-in-oil particles from a mixed solution containing an oil phase and an aqueous phase. The dispersion is then generated by ultrasonic atomization and released to the outside. The water-in-oil particles consist of an outer oil phase and an inner aqueous phase. The aqueous phase contains nucleic acid molecules encapsulated by an organic framework material. The base sequence of the nucleic acid molecules encodes data information.

[0022] In some embodiments, the device further includes a transmission channel that connects the liquid storage tank and the ultrasonic atomizing component.

[0023] In some embodiments, the nucleic acid molecular aerosol generating unit further includes a second power element for releasing the nucleic acid molecular aerosol to the outside.

[0024] In some implementations, the second power element includes a fan.

[0025] In some implementations, the condenser has a hydrophobic inner wall.

[0026] In a sixth aspect, the present invention provides a microfluidic chip including the aforementioned nucleic acid molecular aerosol recovery unit and amplification unit.

[0027] In some implementations, the amplification unit includes an amplification channel, and the condenser is connected to the amplification channel.

[0028] In some implementations, the amplification unit also includes a temperature control component located around the amplification channel.

[0029] In some implementations, the microfluidic chip also includes a sequencing unit.

[0030] A seventh aspect of the present invention provides a data looping method, comprising the following steps: Using the aforementioned data recycling device, nucleic acid molecular aerosols are released through the nucleic acid molecular aerosol generation unit; and / or, nucleic acid molecular aerosols are recovered through the nucleic acid molecular aerosol recovery unit.

[0031] In an eighth aspect, the present invention provides the aforementioned data storage medium, the aforementioned nucleic acid molecular aerosol recovery unit, the aforementioned device, the aforementioned data circulation device, or the aforementioned microfluidic chip in data storage, data transmission, and data reading.

[0032] The beneficial effects of this invention are: This invention is the first to extend nucleic acid molecular data storage to an air medium via aerosols, achieving true carrier-free distributed storage. Aerosols offer excellent suspendability, resulting in long-term preservation of nucleic acid molecular data within particles and fast data loading / access speeds. Furthermore, it offers the following advantages: 1. High density, even considering the density of aerosols dispersed in air as 35 μg / m³. 3 Even with particulate number concentrations in air quality rated as "excellent," its storage density can still reach approximately 15,000 TB / m³. 3 Furthermore, it is also the data transmission rate and bandwidth.

[0033] 2. Energy-free data transmission: Data can be transmitted without the need to build large base stations, relying on natural airflow. This is especially useful in places where electromagnetic waves cannot transmit (such as caves, pristine natural environments, and data transmission in cities after natural disasters), where it can serve as a complementary alternative.

[0034] 3. It can transmit cold data without requiring timely data transmission, and the data volume is extremely large.

[0035] Therefore, this data storage medium can serve as an alternative to electromagnetic wave information transmission in specific environments. Although the transmission speed is slow, the single transmission volume is large. In particular, in the "space" environment mentioned above, the effect of air dilution is limited, which can achieve stable data transmission and utilization.

[0036] In addition, this invention demonstrates for the first time that nucleic acid molecules can simultaneously store and transmit data, breaking the traditional "storage-transmission" separation model; the related device enables the release and recovery of air-controlled nucleic acid molecular aerosols; combined with microfluidic chips, it can realize the collection, amplification and sequencing processes of nucleic acid molecular aerosols for precise data extraction and decoding. Attached Figure Description

[0037] Figure 1 This is a schematic diagram of the data loop device in some embodiments of this application.

[0038] Figure 2 This is a schematic diagram of the structure of a microfluidic chip in some embodiments of this application.

[0039] Figure 3 This is the result of the suspension half-life test of the nano-DNA aerosol in Example 1 of this application.

[0040] Figure 4 This is the recovery rate test result of the airflow control device in Embodiment 2 of this application.

[0041] Figure 5 This is the result of DNA readability analysis of this application.

[0042] Figure 6 These are the test results of how different airflow velocities affect the decapsulation efficiency of nucleic acid molecular aerosols in the embodiments of this application.

[0043] Figure 7These are the test results of how different convection angles affect the deencapsulation efficiency of nucleic acid molecular aerosols in the embodiments of this application.

[0044] Figure description: condenser 110, condenser metal plate 111, condenser controller 112, condenser vent 113, liquid storage tank 120, first power element 130, ultrasonic atomizing component 210, atomizing mesh 211, ultrasonic atomizing component vent 213, atomizing controller 212, second power element 220, transmission channel 300, amplification unit 400, amplification flow channel 410, sample inlet 411, sample outlet 412. Detailed Implementation

[0045] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the application. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains. The terminology used herein is for the purpose of describing embodiments of this application only and is not intended to limit the application. Those skilled in the art will recognize that, with the evolution of technology and the emergence of new application scenarios, the technical solutions provided in the embodiments of this application are equally applicable to similar technical problems.

[0046] In a first aspect, the present invention provides a data storage medium comprising nucleic acid molecular aerosols, wherein the raw materials of the nucleic acid molecular aerosols include water-in-oil particles, the water-in-oil particles comprising an outer oil phase and an inner aqueous phase, the aqueous phase comprising nucleic acid molecules encapsulated by an organic framework material, and the base sequence of the nucleic acid molecules encoding data information.

[0047] The term "nucleic acid molecule" generally refers to a polymer of nucleotides. Nucleotides typically include a base, a sugar, and optionally a monophosphate or polyphosphate group. Bases include one or more of adenine (A), cytosine (C), guanine (G), thymine (T), and other natural or non-natural types of bases such as uracil (U) and hypoxanthine (I). Sugars typically include ribose or deoxyribose, thus the nucleotide is a ribonucleotide or deoxyribonucleotide, and the nucleic acid molecule can be one or more of DNA molecules, RNA molecules, DNA-RNA hybrid molecules, peptide nucleic acids (PNAs), etc. For example, in some embodiments, the nucleic acid molecule aerosol is a DNA aerosol or an RNA aerosol, or a mixture of DNA aerosols and RNA aerosols.

[0048] Raw data can be encoded into the base sequence of nucleic acid molecules using specific methods. These encoding methods include, but are not limited to, direct conversion, linear block coding, fountain coding, convolutional coding, and one or more of these methods. Correspondingly, the base sequence of the nucleic acid molecule can be decoded into the raw data according to the corresponding encoding method.

[0049] The term "aerosol" refers to a sol formed with a solid or liquid as the dispersed phase and a gas as the dispersion medium. Specifically, in the embodiments of this invention, the dispersed phase includes water-in-oil particles. Small aerosol particles may evaporate in the air, leading to size changes, or settle due to rapid aggregation. Therefore, this invention employs a water-in-oil core-shell structure to suppress rapid size changes in nano-aerosol particles.

[0050] In some embodiments, the particle size of the nucleic acid molecular aerosol is 10-500 nm, for example, it can be 10 nm, 20 nm, 30 nm, 40 nm, 50 nm, 60 nm, 70 nm, 80 nm, 90 nm, 100 nm, 150 nm, 200 nm, 250 nm, 300 nm, 350 nm, 400 nm, 450 nm, or 500 nm. When the particle size of the aerosol is controlled within the above range, it can be ensured that it can be suspended in the air for a long time. Understandably, the original water-in-oil particle dispersion is atomized to reduce the particle size, ultimately controlling the particle size of the generated aerosol to be 10~500 nm. Therefore, the particle size of the raw material for water-in-oil particles is larger than that of the aerosol, and can be in the micrometer range, such as 1~100 μm, for example, 1 μm, 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm, 10 μm, 15 μm, 20 μm, 25 μm, 30 μm, 35 μm, 40 μm, 45 μm, 50 μm, 55 μm, 60 μm, 65 μm, 70 μm, 75 μm, 80 μm, 85 μm, 90 μm, 95 μm, 100 μm. In some embodiments, the particle size of the aforementioned aerosol particles or water-in-oil particles can be measured by any of the following methods: dynamic light scattering (DLS), nanoparticle tracer analysis (NTA), laser particle size analysis, or electron microscopy (such as transmission electron microscopy TEM or scanning electron microscopy SEM).

[0051] In some embodiments, the organic framework material includes at least one of metal-organic frameworks (MOFs) and hydrogen-bonded organic frameworks (HOFs). In some embodiments, the metal-organic framework material includes any one of UIO-type, MOF-type, IRMOF-type, ZIF-type, BTC-type, MIL-type, HKUST-type, etc. In some embodiments, the metal-organic framework material includes at least one of UIO-66, UIO-67, UIO-68, UIO-69, MOF-5, MOF-74, MOF-177, MOF-505, MOF-525, MOF-801, IRMOF-1, IRMOF-3, IRMOF-6, IRMOF-8, ZIF-7, ZIF-8, MIL-53, MIL-88, MIL-101, HKUST-1, etc.

[0052] In some embodiments, the oil phase includes at least one of vegetable oil, mineral oil, and silicone oil.

[0053] In some embodiments, the interface between the oil phase and the aqueous phase further includes an ionic polymer material. In some embodiments, the ionic polymer material includes at least one selected from polyethyleneimine, polydiallyldimethylammonium chloride, polyacrylic acid, and sodium polystyrene sulfonate. In some embodiments, the mass-volume percentage of the ionic polymer to the oil phase is 0.1% to 50% w / v, for example, it can be 0.1% w / v, 0.2% w / v, 0.4% w / v, 0.5% w / v, 0.6% w / v, 0.8% w / v, 1% w / v, 2% w / v, 3% w / v, 4% w / v, 5% w / v, 6% w / v, 7% w / v, 8% w / v, 9% w / v, 10% w / v, 12% w / v, 14% w / v, 15% w / v, 16% w / v, 18% w / v, 20% w / v, 22% w / v, 24% w / v, 25% w / v, 26% w / v, 28% w / v, etc. % w / v, 30% w / v, 32% w / v, 34% w / v, 35% w / v, 36% w / v, 38% w / v, 40% w / v, 42% w / v, 44% w / v, 45% w / v, 46% w / v, 48% w / v, 50% w / v. Wherein, the mass-volume percentage of solids to liquids (% w / v) represents the weight in grams of solids per 100 ml of liquid.

[0054] In some embodiments, the interface between the oil phase and the aqueous phase further includes a surfactant. In some embodiments, the surfactant in the oil phase has a mass-volume percentage of 0.01 to 5% w / v, for example, 0.01% w / v, 0.02% w / v, 0.05% w / v, 0.08% w / v, 0.1% w / v, 0.2% w / v, 0.5% w / v, 0.8% w / v, 1% w / v, 2% w / v, 3% w / v, 4% w / v, or 5% w / v. In some embodiments, the surfactant includes a nonionic surfactant. In some embodiments, the surfactant has an HLB value of 3 to 6, for example, 3, 4, 5, or 6. In some embodiments, the surfactant includes at least one of Span derivatives, fatty acid glycerides, polyglycerol fatty acid esters, and polyethers.

[0055] In a second aspect, the present invention provides a method for generating the aforementioned data storage medium, comprising the following steps: forming a dispersion of water-in-oil particles from a mixed solution containing an oil phase and an aqueous phase, and then generating nucleic acid molecular aerosols by ultrasonic atomization.

[0056] In some embodiments, ultrasonic atomization is performed using an ultrasonic atomizer. In some embodiments, the ultrasonic atomizer includes a piezoelectric ceramic plate and a microporous atomizing plate. In some embodiments, the ultrasonic frequency of ultrasonic atomization is 1–10 MHz, for example, 1 MHz, 2 MHz, 3 MHz, 4 MHz, 5 MHz, 6 MHz, 7 MHz, 8 MHz, 9 MHz, or 10 MHz. In some embodiments, the pore size of the microporous atomizing plate is 1–10 μm, for example, 1 μm, 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm, or 10 μm. In some embodiments, ultrasonic atomization includes controlling the ultrasonic frequency to 1–10 MHz and controlling the pore size of the microporous atomizing plate to 1–10 μm. The suspension state of aerosols in air is mainly affected by gravity, aerodynamics, and Brownian motion; therefore, the size of the aerosol usually determines its propagation distance. Based on this, the nanoscale size and particle size distribution of aerosols can be controlled by specific ultrasonic atomization parameters to achieve aerosol particles with good suspension properties. It can be understood that aerosol particles are essentially smaller water-in-oil particles after atomization, and the aqueous phase in water-in-oil particles typically contains multiple nucleic acid molecules encapsulated by organic framework materials.

[0057] In some embodiments, the generation method further includes the step of preparing an aqueous phase containing an organic framework material encapsulated with nucleic acid molecules.

[0058] In some embodiments, this step includes mixing and encapsulating the nucleic acid molecules with the encapsulation solution. In some embodiments, the volume ratio of the nucleic acid molecule solution to the encapsulation solution is 1:5 to 1:20, for example, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, or 1:20.

[0059] In some embodiments, the encapsulation solution typically comprises one or two components. For MOFs, the encapsulation solution comprises a metal salt solution and an organic ligand solution, wherein the molar ratio of the metal salt to the organic ligand can be 1:0.1 to 1:10, for example, 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, or 1:10. In some embodiments, the solvents for the metal salt solution and the organic ligand solution are each independently selected from at least one of water, alcohols (such as methanol or ethanol), and amides (such as N,N-dimethylformamide DMF). In some embodiments, the pH range of the encapsulation solution is adjusted to 4.0–12.0, for example, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10.0, 10.5, 11.0, 11.5, or 12.0. In some embodiments, MOF materials can be synthesized using room temperature stirring, solvothermal methods, microwave-assisted synthesis, or ultrasonic-assisted synthesis. For HOFs, organic ligand solutions are included. In some embodiments, the solvent for the organic ligand solution is selected from at least one of water, methanol, acetonitrile, acetone, and chloroform. In some embodiments, the concentration of the organic ligand solution is from 0.1 mg / mL to 20 mg / mL, for example, it can be 0.1 mg / mL, 0.2 mg / mL, 0.3 mg / mL, 0.4 mg / mL, 0.5 mg / mL, 0.6 mg / mL, 0.7 mg / mL, 0.8 mg / mL, 0.9 mg / mL, 1 mg / mL, 2 mg / mL, 3 mg / mL, 4 mg / mL, 5 mg / mL, 6 mg / mL, 7 mg / mL, 8 mg / mL, 9 mg / mL, 10 mg / mL, 11 mg / mL, 12 mg / mL, 13 mg / mL, 14 mg / mL, 15 mg / mL, 16 mg / mL, 17 mg / mL, 18 mg / mL, 19 mg / mL, or 20 mg / mL. HOFs are mainly formed through self-assembly via hydrogen bonding and π-π stacking interactions between organic ligands, without the participation of metal ions.

[0060] In some embodiments, the encapsulation temperature is 10~80℃, for example, it can be 10℃, 20℃, 30℃, 40℃, 50℃, 60℃, 70℃, or 80℃. In some embodiments, the encapsulation time is 1~1000 s, for example, it can be 1 s, 2 s, 5 s, 10 s, 20 s, 50 s, 100 s, 200 s, 500 s, or 1000 s.

[0061] In some embodiments, the method further includes a step of pre-amplifying the nucleic acid molecules before encapsulation. In some embodiments, the amplification includes at least one of polymerase chain reaction (PCR), isothermal amplification reactions (such as loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), rolling circle amplification (RCA), cross-primer amplification (CPA), strand displacement amplification (SDA), helicase-dependent amplification (HDA), etc.).

[0062] Another aspect of the present invention provides a method for decapsulating nucleic acid molecular aerosols, comprising the following steps: setting up a first gas flow containing nucleic acid molecular aerosols and a second gas flow containing a decapsulation solution, wherein the flow velocities of the first and second gas flows are independently 1~10 m / s (specifically, 1 m / s, 2 m / s, 3 m / s, 4 m / s, 5 m / s, 6 m / s, 7 m / s, 8 m / s, 9 m / s, 10 m / s), and the convection angle between the first and second gas flows is 90~180 degrees (specifically, 90 degrees, 95 degrees, 100 degrees, 105 degrees, 110 degrees, 115 degrees, 120 degrees, 125 degrees, 130 degrees, 135 degrees, 140 degrees, 145 degrees, 150 degrees, 155 degrees, 160 degrees, 165 degrees, 170 degrees, 175 degrees, 180 degrees). The convection angle refers to the angle between the vector represented by the direction of movement of the first gas flow and the vector represented by the direction of movement of the second gas flow.

[0063] By setting the airflow speed and convection angle as described above, the probability of encounter and reaction between aerosol particles is increased. Although nucleic acid aerosol molecules can be stably suspended in the air, passive diffusion and sedimentation alone are insufficient for rapid document access in the air. Therefore, the above method enables the decapsulation of nucleic acid molecules in the air, thereby allowing rapid access to the data information stored on the nucleic acid molecules.

[0064] In some implementations, the second gas flow is generated by atomizing a decapsulation solution. After the first and second gas flows meet in convection, the aerosol particles mix with the decapsulation solution, removing the organic framework material encapsulating the nucleic acid molecules. Therefore, the convection region in the space "exposes" the nucleic acid molecule file for subsequent capture by the chip.

[0065] Depending on the organic framework material, the decapsulation solution can have different compositions, as long as it can decompose the organic framework material. In some embodiments, for MOF framework materials, the decapsulation solution includes EDTA or an acidic buffer (pH, for example, 2-6.9). In some embodiments, for HOF framework materials, the decapsulation solution includes an alkaline buffer (pH, for example, 8-13).

[0066] Another aspect of the present invention provides a nucleic acid molecular aerosol recovery unit, as referenced. Figure 1 The system includes a first power element 130 and a condenser 110. The first power element 130 is used to recover gas containing nucleic acid molecular aerosol from the outside. The raw material of the nucleic acid molecular aerosol includes water-in-oil particles, which include an outer oil phase and an inner water phase. The water phase includes nucleic acid molecules encapsulated by an organic framework material. The base sequence of the nucleic acid molecules encodes data information. The condenser 110 is used to liquefy the gas to obtain a solution containing water-in-oil particles.

[0067] In some embodiments, the nucleic acid molecular aerosol recovery unit further includes a storage tank 120, which is connected to a condenser 110, for collecting a solution containing water-in-oil particles. In some embodiments, a transmission channel 300 for mutual communication is provided between the storage tank 120 and the condenser 110.

[0068] In some embodiments, the first power element 130 includes a first fan. In some embodiments, the nucleic acid molecular aerosol recovery unit also includes an air inlet, with the first fan located at the air inlet.

[0069] In some embodiments, the condenser 110 includes a condensing metal plate 111. In some embodiments, the condensing metal plate 111 is made of at least one element selected from copper, aluminum, titanium, nickel, and iron, either as a single element or an alloy, such as copper alloys, copper-nickel alloys, aluminum alloys, titanium alloys, nickel alloys, stainless steel, carbon steel, etc. In some embodiments, the condenser 110 also includes a condenser controller 112 connected to the condensing metal plate 111. In some embodiments, the condenser controller 112 is connected to a power source. In some embodiments, the condensing metal plate 111 is annular. In some embodiments, the condenser 110 also has a condenser vent 113.

[0070] In another aspect, the present invention provides an apparatus comprising the aforementioned nucleic acid molecular aerosol recovery unit.

[0071] In another aspect, the present invention provides a data looping device, with reference to Figure 1It includes the aforementioned nucleic acid molecular aerosol recovery unit and nucleic acid molecular aerosol generation unit. The nucleic acid molecular aerosol generation unit includes an ultrasonic atomization component 210, which is used to form a dispersion of water-in-oil particles from a mixed solution containing an oil phase and an aqueous phase. Then, nucleic acid molecular aerosols are generated by ultrasonic atomization and released to the outside. The water-in-oil particles include an outer oil phase and an inner aqueous phase. The aqueous phase includes nucleic acid molecules encapsulated by an organic framework material. The base sequence of the nucleic acid molecules encodes data information.

[0072] In some embodiments, the ultrasonic atomizing assembly 210 includes an atomizing mesh 211. In some embodiments, the ultrasonic atomizing assembly 210 further includes an atomizing controller 212. In some embodiments, the atomizing controller 212 is connected to a power source. In some embodiments, the ultrasonic atomizing assembly 210 also has an ultrasonic atomizing assembly vent 213.

[0073] In some embodiments, the device further includes a transmission channel 300 that connects the liquid storage tank 120 and the ultrasonic atomizing component 210.

[0074] In some embodiments, the nucleic acid aerosol generating unit further includes a second power element 220 for releasing nucleic acid aerosols to the outside. In some embodiments, the second power element 220 includes a second fan. In some embodiments, the nucleic acid aerosol generating unit has an air outlet, and the second fan is located at the air outlet.

[0075] In some implementations, the condenser has a hydrophobic inner wall.

[0076] In some implementations, reference Figure 1 The data circulation device includes a nucleic acid aerosol generation unit, a nucleic acid aerosol recovery unit, and a transmission channel between the two units. The ultrasonic atomizing component in the nucleic acid aerosol generation unit is located inside the air outlet. The nucleic acid aerosol generated by the ultrasonic atomizing component is diffused to the outside by a fan to release the aerosol. The nucleic acid aerosol recovery unit has an air inlet, ensuring unidirectional airflow within the space. Air from the outside space enters the nucleic acid aerosol recovery unit after passing through the air inlet, and comes into contact with a condenser with a hydrophobic surface, causing the air to liquefy and collect in a storage tank, thus completing the recovery of the nucleic acid aerosol. This data circulation device can release nucleic acid aerosols encoding data information into the air and maintain air data storage within the device through air circulation, while simultaneously recovering airborne nucleic acid aerosols to pause data sharing.

[0077] Another aspect of the present invention provides a microfluidic chip, with reference to Figure 2The system includes the aforementioned nucleic acid aerosol recovery unit and amplification unit 400. In the nucleic acid aerosol recovery unit, external gas is collected via a first power element (not shown), and a condenser 110 further collects and liquidates the nucleic acid aerosols from the external gas. In some embodiments, the condenser 110 is located on one side of the first power element. In some embodiments, the condenser 110 includes a condensing metal plate 111. In some embodiments, the condenser 110 also includes a condenser controller 112. In some embodiments, air enters from one side of the condensing metal plate 111 and exits from the other side.

[0078] In some embodiments, the amplification unit 400 includes an amplification channel 410, and a condenser 110 is connected to the amplification channel 410. In some embodiments, the amplification channel 410 includes any one of a serpentine channel, a spiral channel, etc. In some embodiments, the amplification channel 410 has inlet ports 411 and outlet ports 412 at both ends. In some embodiments, there are multiple inlet ports 411. In some embodiments, the condenser 110 is connected to one of the inlet ports 411 to introduce liquefied aerosol products into the amplification channel 410. In some embodiments, the amplification channel 410 also includes other inlet ports 411 for supplying other amplification materials (such as dNTPs, enzymes, primers, etc.). In some embodiments, the amplification unit 400 also includes a temperature control component located around the amplification channel. In some embodiments, the microfluidic chip also includes a sequencing unit. In some embodiments, the outlet port is connected to the sequencing unit. In some embodiments, the sequencing unit performs sequencing using any one of first-generation sequencing, second-generation sequencing, or third-generation sequencing. Among them, first-generation sequencing technologies include Maxam-Gilbert sequencing, Sanger dideoxy sequencing, pyrosequencing, fluorescence automated sequencing, and hybridization sequencing; second-generation sequencing technologies include 454 RocheGS FLX, Illumina Solexa, SOLiD, Ion Torrent, and BGISEQ; and third-generation sequencing technologies include HeliScope, PacBioHiFi, PacBio CLR, ONT, and Cyclone WT.

[0079] Another aspect of the present invention provides a data looping method, comprising the following steps: Using the aforementioned data recycling device, nucleic acid molecular aerosols are released through the nucleic acid molecular aerosol generation unit; and / or, nucleic acid molecular aerosols are recovered through the nucleic acid molecular aerosol recovery unit.

[0080] Another aspect of the present invention provides the application of the aforementioned data storage medium, the aforementioned nucleic acid molecular aerosol recovery unit, the aforementioned device, the aforementioned data circulation device, or the aforementioned microfluidic chip in data storage, data transmission, and data reading.

[0081] Example 1 This embodiment provides an aerosol with a water-in-oil core-shell structure.

[0082] The oligo pool (3210 sequences of 140 nt each were obtained by encoding a 18 KB image file with DNA Fountain Code, and then Twist was commissioned to produce the final oligo pool with a concentration of 0.5 ng / μL) was first diluted 100 times with nuclease-free water, and PCR amplification was performed using a multiplex PCR kit (Beyotime, D7301M) according to the instructions. The amplified products were purified and then quantified using Qubit fluorescence.

[0083] The amplification product, precursor solution A (aqueous solution of zinc acetate, 40 mM), and precursor solution B (aqueous solution of 2-methylimidazole, 160 mM) were mixed at a volume ratio of 1:4.5:4.5 and stirred at 25 °C for 15 min to complete encapsulation, resulting in an aqueous solution of the nucleic acid molecule DNA@ZIF-8 encapsulated with organic framework material.

[0084] The aqueous solution was mixed with the oil phase (vegetable oil) and 50% w / v polyethyleneimine and 5% w / v Span80 (HLB4.7) based on the oil phase, with a mass-volume percentage of DNA@ZIF-8 of 10% w / v. The mixture was then atomized using an ultrasonic nebulizer (ultrasonic frequency 5 MHz, microporous nebulizer pore size 5 μm) to form a nano-DNA aerosol.

[0085] Example 2 This embodiment provides a microfluidic chip, including an air enrichment module, a PCR module, and a sequencing module.

[0086] The air enrichment module is equipped with a miniature fan to collect external gases, and a miniature condenser is integrated at the bottom of the fan to collect and liquidate DNA aerosols in the air.

[0087] The PCR module utilizes a conventional soft photolithography method to fabricate a serpentine-structured polydimethylsiloxane (PDMS) flow channel. A Peltier thermoelectric module is integrated beneath the flow channel for programmed rapid heating and cooling. The flow channel features two inlet ports and one outlet port. The inlet ports are used to introduce aerosol liquefaction products and PCR reaction buffer, respectively, while the outlet port carries out the PCR products.

[0088] The sequencing module uses the Oxford TraxION device for automated library construction and the Oxford SmidgION portable sequencer for data readout.

[0089] The three modules are connected via a flexible hose structure: a micro condenser tube connects to the inlet port of the PDMS channel, and the outlet port of the PDMS channel connects to the inlet port of the Oxford TraxION. A fan applies air pressure within the channel to drive the liquid.

[0090] Example 3: Aerosol Size Test Aerosols were prepared according to Example 1, and six different sizes (10 nm, 50 nm, 100 nm, 500 nm, 1000 nm, and 5000 nm) of nano-DNA aerosol particles were prepared by adjusting the ultrasonic atomization frequency and the pore size of the microporous atomizing sheet. The prepared nano-DNA aerosol particles were blown out by an adjustable-speed fan placed behind the ultrasonic atomizer, ensuring that the aerosols propagated in one direction at a specific speed, while controlling the ambient humidity at 50% RH. Specifically, the 10 nm, 50 nm, 100 nm, and 500 nm aerosol particles were prepared using a 500 nm pore size microporous atomizing sheet at frequencies of approximately 50 MHz, 30 MHz, 5 MHz, and 1 MHz, respectively. The 1000 nm and 5000 nm aerosol particles were prepared using a 5000 nm pore size microporous atomizing sheet at frequencies of approximately 20 MHz and 5 MHz, respectively.

[0091] The microfluidic chip described in Example 2 was used to sample at a distance of 5 m from the release port, with a sampling interval of 2 hours. Subsequently, using GAPDH as an internal control, real-time quantitative PCR (qPCR) was performed. -ΔΔCt The method analyzes the DNA content in the sample, obtains the change curve of DNA content at the detection site over time, and calculates the suspension half-life to obtain the suspension time of the nano-DNA aerosol. The results are as follows: Figure 3 As can be seen from the figure, the suspension time of aerosols is longest when the size is between 100 and 500 nm.

[0092] Example 4 This embodiment provides a data looping device, referenced... Figure 1The device comprises a tubular structure open at both ends. One end is an air inlet equipped with a fan and a condenser with a hydrophobic surface, connected to a liquid storage tank on the other side of the condenser. The other end is an air outlet equipped with a fan and an ultrasonic atomizer, which is connected to the liquid storage tank via a transmission pipe. Aerosol particles from the air are drawn into the device through the air inlet. The recovered aerosol particles are stored in the liquid storage tank and liquefied into a solution. The solution is then further atomized by the atomizer and released back into the space outside the data circulation device by the fan.

[0093] Example 5: Recovery Rate Test At 20 m 3 A known amount of nano-DNA aerosol was released into a space, and a data circulation device as described in Example 4 was installed within the space. The nano-DNA aerosol was recovered using the data circulation device after different time intervals, and the DNA concentration was tested by qPCR to calculate the recovery rate of the airflow control device. Recovery rate = DNA detection value / initial DNA amount × 100%.

[0094] The results are as follows Figure 4 As shown in the figure, the recovery rate decreases continuously over time.

[0095] Example 6: Sensitivity Testing of Microfluidic Chips To test the sensitivity of the microfluidic chip in Example 2, the readability of the chip's data was tested at different concentrations in air (0.01, 0.05, 0.1, 1, 5, 10, 50, 100 ppm). The test DNA consisted of PCR products from a pool of ten oligonucleotides synthesized using high-throughput oligonucleotide synthesis technology, with a total length of 140 bp (100 bp data loading region and 40 bp PCR primer binding region). The readability of the extracted coding DNA was tested using the microfluidic chip in Example 2 (the fan in the microfluidic chip rotated at 100 rpm for 1 min, collecting approximately 20 μL of liquid liquefied from aerosol particles; the total time was approximately 40 minutes). The results are as follows: Figure 5 As can be seen from the figure, the data reading accuracy can reach 100% when the concentration is 0.1 ppm.

[0096] Example 7: Effect of different airflow velocities on the deencapsulation efficiency of nucleic acid molecular aerosols Using the aerosol generated by atomization in Example 1 as the first gas flow, and citrate buffer (pH 4.5) atomized as the second gas flow, the first and second gas flows were set to collide at a 180° angle. After a stable collision gas flow was formed, sediment was collected using a PCR tube 20 cm below the collision point for 1 minute. DNA was extracted from the sediment, and the DNA concentration was quantitatively analyzed using qPCR. The deencapsulation efficiency was calculated as: product DNA concentration / aerosol DNA concentration × 100%.

[0097] The convection velocities of the first and second airflows were set to 0.5 m / s, 1 m / s, 5 m / s, and 10 m / s, respectively, to investigate the deencapsulation effect of nucleic acid aerosols under different airflow velocities. The results are as follows: Figure 6 As shown in the figure, when the convection velocity changes from 0.5 to 10 m / s, the desealment efficiency first increases and then decreases. At around 5 m / s, the desealment efficiency can reach close to 100%.

[0098] Example 8: Effect of different convection angles on the deencapsulation efficiency of nucleic acid molecules in aerosols Referring to Example 7, with both the first and second airflow velocities fixed at 5 m / s, and airflow convection angles set to 45°, 90°, 135°, and 180° respectively, the effect of the angle on the desealment effect was investigated. The results are as follows: Figure 7 As shown in the figure, when the convection angle is above 90 degrees, the desealment efficiency is relatively high, especially when it is above 135 degrees, the desealment efficiency can basically reach 100%.

[0099] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A data storage medium, characterized in that, The aerosol includes nucleic acid molecules, the raw material of which includes water-in-oil particles, the water-in-oil particles comprising an outer oil phase and an inner aqueous phase, the aqueous phase comprising nucleic acid molecules encapsulated by an organic framework material, the base sequence of which encodes data information; Optionally, the particle size of the nucleic acid molecular aerosol is 10~500 nm.

2. The data storage medium according to claim 1, characterized in that, The organic framework material includes at least one of metal-organic framework materials, covalent organic framework materials, and hydrogen-bonded organic framework materials; Optionally, the metal-organic framework material includes at least one of HKUST-1, ZIF-8, ZIF-67, and UIO-66.

3. The data storage medium according to claim 1, characterized in that, The oil phase includes at least one of vegetable oil, mineral oil, and silicone oil; Optionally, the interface between the oil phase and the aqueous phase further includes an ionic polymer material; Optionally, the ionic polymer material includes at least one of polyethyleneimine, polydiallyldimethylammonium chloride, polyacrylic acid, and sodium polystyrene sulfonate; Optionally, the mass-volume percentage of the ionic polymeric material to the oil phase is 0.1~50% w / v; Optionally, the interface between the oil phase and the aqueous phase further includes a surfactant; Optionally, the surfactant has a mass-volume percentage of 0.01 to 5% w / v relative to the oil phase.

4. The method for generating a data storage medium according to any one of claims 1 to 3, characterized in that, Includes the following steps: A mixed solution containing an oil phase and an aqueous phase is formed into a dispersion of water-in-oil particles, which is then generated into nucleic acid molecular aerosols by ultrasonic atomization. Optionally, the ultrasonic frequency of the ultrasonic atomization is 1~10 MHz; Optionally, the pore size of the ultrasonic atomizing microporous atomizing sheet is 1~10 μm.

5. A nucleic acid molecular aerosol recovery unit, characterized in that, include: A first power element is used to recover gas containing the nucleic acid molecule aerosol from the outside. The raw material of the nucleic acid molecule aerosol includes water-in-oil particles. The water-in-oil particles include an outer oil phase and an inner water phase. The water phase includes nucleic acid molecules encapsulated by an organic framework material. The base sequence of the nucleic acid molecules encodes data information. A condenser for liquefying the gas to obtain a solution containing the water-in-oil particles; Optionally, the nucleic acid molecular aerosol recovery unit further includes a storage tank, which is connected to the condenser and is used to collect a solution containing the water-in-oil particles; Optionally, the first power element includes a first fan.

6. An apparatus, characterized in that, Includes the nucleic acid molecular aerosol recovery unit as described in claim 5.

7. A data looping device, characterized in that, The data recycling device includes the nucleic acid molecular aerosol recovery unit as described in claim 5, and: A nucleic acid aerosol generation unit includes an ultrasonic atomization component for forming a dispersion of water-in-oil particles from a mixed solution containing an oil phase and an aqueous phase. The dispersion is then generated by ultrasonic atomization and released to the outside. The water-in-oil particles consist of an outer oil phase and an inner aqueous phase. The aqueous phase includes nucleic acid molecules encapsulated by an organic framework material. The base sequence of the nucleic acid molecules encodes data information. Optionally, the device further includes a transmission channel that connects the liquid storage tank and the ultrasonic atomizing component; Optionally, the nucleic acid molecular aerosol generating unit further includes a second power element for releasing the nucleic acid molecular aerosol to the outside; Optionally, the second power element includes a second fan; Optionally, the condenser has a hydrophobic inner wall.

8. A microfluidic chip, characterized in that, The microfluidic chip includes the nucleic acid molecular aerosol recovery unit and the amplification unit as described in claim 5; Optionally, the amplification unit includes an amplification channel, and the condenser is connected to the amplification channel; Optionally, the amplification unit further includes a temperature control component located around the amplification channel; Optionally, the microfluidic chip further includes a sequencing unit.

9. A data looping method, characterized in that, Includes the following steps: Using the data recycling device of claim 7, the release of nucleic acid molecular aerosols is completed through the nucleic acid molecular aerosol generation unit; and / or, the recovery of nucleic acid molecular aerosols is completed through the nucleic acid molecular aerosol recovery unit.

10. The data storage medium according to any one of claims 1 to 3, and the nucleic acid molecular aerosol recovery unit according to claim 5. The application of the device of claim 6, the data circulation device of claim 7, or the microfluidic chip of claim 8 in data storage, data transmission, and data reading.

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