Method for planting desert cistanche by inoculating haloxylon ammodendron

Abstract

The application discloses a technical field of plant planting, and relates to a Haloxylon ammodendron inoculation Cistanche deserticola planting method, which comprises the following steps: seed pretreatment, soil improvement, inoculation technology, environment regulation, disease and pest control, harvesting and processing; through the steps of seed pretreatment, soil improvement, inoculation technology and environment regulation, the yield and quality of Cistanche deserticola are improved. Ecological restoration: the planting of Haloxylon ammodendron helps to prevent wind and sand, improve soil structure and promote ecological restoration in desertification areas. Soil fertility improvement: through the application of organic fertilizer and microbial agent, the soil fertility is improved, and a good soil environment is provided for the growth of Haloxylon ammodendron and Cistanche deserticola. Water and soil loss reduction: the root system of Haloxylon ammodendron can fix soil and reduce water and soil loss, and soil resources are protected. Sustainable utilization: the method helps to realize the sustainable utilization of Cistanche deserticola resources and reduce the damage to the natural environment.

Description

Technical Field

[0001] This invention relates to the field of plant cultivation technology, and in particular to a method for inoculating desert Cistanche deserticola onto Haloxylon ammodendron trees. Background Technology

[0002] Cistanche deserticola is a perennial rooted parasitic herb of the Orobanchaceae family. It is also known as Dayun, Congrong, Dijing, and Jinsun. It is a precious Chinese medicinal material with unique medicinal functions and high economic development value. It is commonly known as desert ginseng or desert golden bamboo shoot. Cistanche deserticola grows by parasitizing the roots of Haloxylon ammodendron and Tamarix chinensis. Haloxylon ammodendron and Tamarix chinensis are excellent tree species for windbreak and sand control, and for improving the ecological environment. They have been used in my country for over two thousand years. Their hosts are excellent sand-fixing and sand-stabilizing tree species such as Haloxylon ammodendron and Tamarix chinensis. The main parasitic plant of Cistanche deserticola is Haloxylon ammodendron forests growing in the desert. Cistanche deserticola grown by inoculation is called desert Cistanche deserticola, and it is the highest quality Cistanche deserticola product. Developing Haloxylon ammodendron forests and developing the sand industry using desert Haloxylon ammodendron forests as hosts can not only effectively implement large-scale sand control, land greening, improve the ecological environment, and build a beautiful China, but also solve the problem of Cistanche deserticola medicinal resources and meet the sustainable development needs of the traditional Chinese medicine industry. Traditional desert Cistanche deserticola cultivation relies on natural inoculation, which is difficult to meet market demand and puts pressure on the environment. Furthermore, the survival rate of inoculated desert Cistanche deserticola is low. Therefore, there is currently a lack of a method for planting desert Cistanche deserticola by inoculating Haloxylon ammodendron trees to solve the above problems. Summary of the Invention

[0003] The purpose of this invention is to address the shortcomings of existing technologies by proposing a method for planting desert Cistanche deserticola by inoculating Haloxylon ammodendron trees.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] A method for cultivating desert Cistanche deserticola by inoculating Haloxylon ammodendron trees, the method comprising the following steps:

[0006] S1: Seed pretreatment:

[0007] Soaking Haloxylon ammodendron seeds in a suitable concentration of growth hormone solution can promote seed germination.

[0008] S2: Soil Improvement

[0009] Organic fertilizers and microbial agents are applied in areas where Haloxylon ammodendron is planted to improve soil structure and fertility;

[0010] S3: Inoculation Technique

[0011] After the Haloxylon ammodendron trees have grown to a certain height, seeds of Cistanche deserticola are inoculated near the roots of the Haloxylon ammodendron trees using minimally invasive techniques.

[0012] S4: Environmental Control

[0013] Based on the growth requirements of Haloxylon ammodendron and Cistanche deserticola, adjust the irrigation system and shading facilities to maintain suitable soil moisture and temperature;

[0014] S5: Pest and Disease Control:

[0015] Regularly check the growth of Haloxylon ammodendron and Cistanche deserticola, and promptly identify and treat diseases and pests;

[0016] S6: Harvesting and Processing

[0017] The desert Cistanche deserticola is harvested when it matures, and then cleaned, dried, and packaged according to the processing techniques.

[0018] Preferably, step S1, seed preprocessing, further includes the following steps:

[0019] S1: Screening: Select healthy, plump Haloxylon ammodendron seeds;

[0020] S2: Disinfection: Use a suitable disinfectant to disinfect the surface of the seeds to reduce the occurrence of diseases.

[0021] Preferably, step S2, soil improvement, further includes the following steps:

[0022] S1: pH adjustment: Adjust the pH value of the soil by applying an appropriate amount of lime or ferrous sulfate.

[0023] S2: Organic matter addition: Add appropriate amounts of organic matter, such as compost and green manure, to increase the organic matter content of the soil.

[0024] Preferably, step S3, the inoculation technique, further includes the following steps:

[0025] S1: Inoculation site selection: Select a healthy area near the roots of the Haloxylon ammodendron tree for inoculation to improve the success rate of inoculation;

[0026] S2: Inoculation Depth Control: Control the inoculation depth to ensure contact between the seeds of Cistanche deserticola and the roots of Haloxylon ammodendron.

[0027] Preferably, step S4, environmental control, further includes the following steps:

[0028] S1: Automated irrigation system: Automatically adjusts irrigation volume based on data from soil moisture sensors;

[0029] S2: Intelligent Shading System: Automatically adjusts the opening and closing of the shading net based on data from light intensity and temperature sensors.

[0030] Preferably, step S5, pest and disease control, further includes the following steps:

[0031] S1: Biological pesticides: Using biological pesticides to replace chemical pesticides and reduce the impact on the environment;

[0032] S2: Natural Enemy Release: Release natural enemy insects, such as ladybugs, to control pest populations.

[0033] Preferably, step S6, harvesting and processing, further includes the following steps:

[0034] S1: Harvest time determination: The optimal harvest time is determined based on the growth cycle and medicinal component content of desert Cistanche deserticola;

[0035] S2: Processing standardization: Establish standardized cleaning, drying and packaging processes to ensure product quality.

[0036] Beneficial Effects: This invention provides a method for inoculating *Cistanche deserticola* onto *Haloxylon ammodendron*, improving yield and quality. Through seed pretreatment, soil improvement, inoculation techniques, and environmental control, the yield and quality of *Cistanche deserticola* are enhanced. Ecological Restoration: Planting *Haloxylon ammodendron* helps prevent wind erosion and improve soil structure, promoting ecological restoration in desertified areas. Improved Soil Fertility: The application of organic fertilizers and microbial agents improves soil fertility, providing a favorable soil environment for the growth of both *Haloxylon ammodendron* and *Cistanche deserticola*. Reduced Soil Erosion: The root system of *Haloxylon ammodendron* can stabilize the soil, reducing soil erosion and protecting soil resources. Sustainable Utilization: This method contributes to the sustainable utilization of *Cistanche deserticola* resources, reducing damage to the natural environment. Detailed Implementation

[0037] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0038] Example 1

[0039] This embodiment provides a technical solution: a method for planting desert Cistanche deserticola by inoculating Haloxylon ammodendron trees, the method comprising the following steps:

[0040] S1: Seed pretreatment:

[0041] Soaking Haloxylon ammodendron seeds in a suitable concentration of growth hormone solution can promote seed germination.

[0042] S2: Soil Improvement

[0043] Organic fertilizers and microbial agents are applied in areas where Haloxylon ammodendron is planted to improve soil structure and fertility;

[0044] S3: Inoculation Technique

[0045] After the Haloxylon ammodendron trees have grown to a certain height, seeds of Cistanche deserticola are inoculated near the roots of the Haloxylon ammodendron trees using minimally invasive techniques.

[0046] S4: Environmental Control

[0047] Based on the growth requirements of Haloxylon ammodendron and Cistanche deserticola, adjust the irrigation system and shading facilities to maintain suitable soil moisture and temperature;

[0048] S5: Pest and Disease Control:

[0049] Regularly check the growth of Haloxylon ammodendron and Cistanche deserticola, and promptly identify and treat diseases and pests;

[0050] S6: Harvesting and Processing

[0051] The desert Cistanche deserticola is harvested when it matures, and then cleaned, dried, and packaged according to the processing techniques.

[0052] In practice, this method can promote ecological restoration in desertified areas, improve soil fertility, and reduce soil erosion. It not only increases the yield and quality of desert Cistanche deserticola but also contributes to the ecological restoration of desertified areas and the sustainable utilization of medicinal resources. This method has broad application prospects and market potential.

[0053] Example 2

[0054] Based on Example 1, the seed preprocessing step S1 further includes the following steps:

[0055] S1: Screening: Select healthy, plump Haloxylon ammodendron seeds;

[0056] S2: Disinfection: Use a suitable disinfectant to disinfect the surface of the seeds to reduce the occurrence of diseases.

[0057] More specifically, seed pretreatment involves selecting healthy, plump Haloxylon ammodendron seeds, screening them to remove impurities and incomplete seeds, and then soaking the screened seeds in a solution containing 0.1% growth hormone for 24 hours to promote germination. Finally, the seeds are surface-sterilized with a 0.5% hydrogen peroxide solution for 30 minutes to reduce disease occurrence.

[0058] The harvested *Cistanche deserticola* seeds were sun-dried outdoors for four hours, then placed in a container with a specified percentage of Bihu (a plant growth regulator), a specified concentration of Duoshou (an insecticide), and a specified concentration of plant rooting agent. The seeds were treated with these agents for twenty-four hours. Seed packet preparation: The treated *Cistanche deserticola* seeds were removed and drained, then placed in another container with fine sand and a specified proportion of insect repellent powder. The mixture was thoroughly stirred to ensure the seeds were evenly coated with the sand and powder. The dormancy-breaking seeds were then added to a fully automatic packaging machine, which automatically packaged the seeds into packets six centimeters long, three centimeters wide, and two centimeters thick. Each packet contained 180 to 220 *Cistanche deserticola* seeds.

[0059] Example 3

[0060] Based on the above embodiments, step S2, soil improvement, specifically includes the following steps:

[0061] S1: pH adjustment: Adjust the pH value of the soil by applying an appropriate amount of lime or ferrous sulfate.

[0062] S2: Organic matter addition: Add appropriate amounts of organic matter, such as compost and green manure, to increase the organic matter content of the soil.

[0063] More specifically, soil tests were conducted in the planting area of ​​Haloxylon ammodendron to determine the pH value to be 7.5. An appropriate amount of lime was applied to adjust the pH to 6.5-7.0. 5 tons / ha of compost and 2 tons / ha of green manure were added to the soil to increase its organic matter content and improve its structure. A relatively flat area with sandy or sandy loam soil and a groundwater depth of 2 meters was selected. The afforestation site was leveled mechanically or manually, with a slope of 10°. Drip irrigation was installed, and the soil was watered the day before planting, with a moisture depth of 40-60 cm. Ditches were mechanically dug, 30-50 cm deep, with a spacing of 3-4 meters. One-year-old, high-quality, robust seedlings were selected and placed in the trenches, with a spacing of 1 meter × 3-4 meters. Sand was backfilled, and after each third of the soil was filled, the seedlings were tamped down horizontally and vertically with both feet. Then, the seedlings were gently lifted from the middle of their main stem until they felt loose.

[0064] The chain-type Cistanche deserticola trenching, sowing, and strip-laying planting machine is used to open trenches, controlling the depth of the trenches in the planting area to be between 0.6 meters and 1.5 meters, while the width of the trenches is controlled to be between 35 centimeters and 45 centimeters.

[0065] Example 4

[0066] Based on the above embodiments, the inoculation technique in step S3 further includes the following steps:

[0067] S1: Inoculation site selection: Select a healthy area near the roots of the Haloxylon ammodendron tree for inoculation to improve the success rate of inoculation;

[0068] S2: Inoculation Depth Control: Control the inoculation depth to ensure contact between the seeds of Cistanche deserticola and the roots of Haloxylon ammodendron.

[0069] More specifically, evenly sow fine sand mixed with Cistanche deserticola seeds along the bottom of the furrow at a rate of 10g / m, and rake it to a depth of 3-8cm; cover with 10-15cm of soil and rake it flat; place a 15cm wide strip of Cistanche deserticola seeds with the back side pressed against the furrow wall and the front side facing the host, lay it flat, cover with 10-15cm of soil, and rake it flat.

[0070] Partial root cutting is performed on the pre-planted Haloxylon ammodendron trees, and the trees with cut roots are then planted in the trench. When the Haloxylon ammodendron trees reach a height of 30 cm, seeds of Cistanche deserticola are inoculated into healthy areas near the roots using a minimally invasive technique. The inoculation depth is controlled at 5-10 cm to ensure contact between the Cistanche deserticola seeds and the Haloxylon ammodendron roots, thereby improving the inoculation success rate. The Cistanche deserticola seed packets are then planted into the soil below the Haloxylon ammodendron trees through a seed delivery tube. In April-May and October-November of the second year, dig trenches 20-30cm away from one side of the Haloxylon ammodendron forest belt, with a trench width of 20cm and a depth of 65-70cm; take dry fine sand with a total salt content of 0.3% and a pH value of 8.5 for later use; soak Cistanche deserticola seeds in a potassium permanganate solution of 1g L^-1-3g L^-1 for 15-20min, air dry them, and then mix them into the fine sand prepared in step b. The mixing ratio of Cistanche deserticola seeds to fine sand is 1:100.

[0071] Example 5

[0072] Based on the above embodiments, step S4, environmental control, further includes the following steps:

[0073] S1: Automated irrigation system: Automatically adjusts irrigation volume based on data from soil moisture sensors;

[0074] S2: Intelligent Shading System: Automatically adjusts the opening and closing of the shading net based on data from light intensity and temperature sensors.

[0075] More specifically, soil moisture sensors and light intensity sensors are installed to automatically adjust irrigation levels and the opening and closing of shade nets based on the data. Soil moisture is maintained at 40%-60%, and the temperature at 15-25℃ to meet the growth needs of Haloxylon ammodendron and Cistanche deserticola.

[0076] Example 6

[0077] Based on the above embodiments, step S5, pest and disease control, further includes the following steps:

[0078] S1: Biological pesticides: Using biological pesticides to replace chemical pesticides and reduce the impact on the environment;

[0079] S2: Natural Enemy Release: Release natural enemy insects, such as ladybugs, to control pest populations.

[0080] More specifically, use biological pesticides, such as 0.3% Bt fungicide, to replace chemical pesticides and reduce environmental impact. Release natural enemy insects, such as ladybugs, at a rate of 5,000 per hectare to control pest populations.

[0081] Example 7

[0082] Based on the above embodiments, step S6, harvesting and processing, further includes the following steps:

[0083] S1: Harvest time determination: The optimal harvest time is determined based on the growth cycle and medicinal component content of desert Cistanche deserticola;

[0084] S2: Processing standardization: Establish standardized cleaning, drying and packaging processes to ensure product quality.

[0085] More specifically, based on the growth cycle and medicinal component content of *Cistanche deserticola*, the optimal harvest time is determined to be the autumn of the second year after planting. Standardized cleaning, drying, and packaging processes are established to ensure product quality. Running water is used for cleaning, natural air drying or low-temperature drying is used, and breathable packaging materials are used.

[0086] Ecological benefits: By inoculating Haloxylon ammodendron with Cistanche deserticola, the ecological restoration of desertified areas can be promoted, soil fertility can be improved, and soil erosion can be reduced. Soil fertility and soil erosion will be monitored regularly, and the growth status of Haloxylon ammodendron and Cistanche deserticola will be recorded to assess the ecological benefits.

[0087] This invention provides a method for inoculating Haloxylon ammodendron with Cistanche deserticola. The method involves soaking the Haloxylon ammodendron in a growth hormone solution and surface disinfection to improve seed germination rate and reduce disease occurrence, laying a foundation for subsequent planting. Applying organic fertilizer and microbial agents adjusts soil pH, increases soil organic matter content, and improves soil structure, creating favorable conditions for the growth of both Haloxylon ammodendron and Cistanche deserticola. After the Haloxylon ammodendron reaches a certain height, Cistanche deserticola seeds are inoculated near the roots of the Haloxylon ammodendron using a minimally invasive technique, utilizing the Haloxylon ammodendron as a host to promote Cistanche deserticola growth. Based on the growth needs of both Haloxylon ammodendron and Cistanche deserticola, an automated irrigation system and an intelligent shading system regulate soil moisture and temperature to provide a suitable environment for plant growth. Regularly checking the growth status of both Haloxylon ammodendron and Cistanche deserticola and using biological control methods such as biological pesticides and the release of natural enemies reduces the occurrence of pests and diseases. The desert Cistanche deserticola is harvested when it matures, and then cleaned, dried, and packaged according to standardized processes to ensure product quality.

[0088] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A method for planting *Cistanche deserticola* by inoculating *Haloxylon ammodendron* trees, characterized in that: The method includes the following steps: S1: Seed pretreatment: Soaking Haloxylon ammodendron seeds in a suitable concentration of growth hormone solution can promote seed germination. S2: Soil Improvement Organic fertilizers and microbial agents are applied in areas where Haloxylon ammodendron is planted to improve soil structure and fertility; S3: Inoculation Technique After the Haloxylon ammodendron trees have grown to a certain height, seeds of Cistanche deserticola are inoculated near the roots of the Haloxylon ammodendron trees using minimally invasive techniques. S4: Environmental Control Based on the growth requirements of Haloxylon ammodendron and Cistanche deserticola, adjust the irrigation system and shading facilities to maintain suitable soil moisture and temperature; S5: Pest and Disease Control: Regularly check the growth of Haloxylon ammodendron and Cistanche deserticola, and promptly identify and treat diseases and pests; S6: Harvesting and Processing The desert Cistanche deserticola is harvested when it matures, and then cleaned, dried, and packaged according to the processing techniques.

2. The method for planting *Cistanche deserticola* by inoculating *Haloxylon ammodendron* on *Haloxylon ammodendron* according to claim 1, characterized in that: The seed pretreatment step S1 further includes the following steps: S1: Screening: Select healthy, plump Haloxylon ammodendron seeds; S2: Disinfection: Use a suitable disinfectant to disinfect the surface of the seeds to reduce the occurrence of diseases.

3. The method for planting *Cistanche deserticola* by inoculating *Haloxylon ammodendron* on *Haloxylon ammodendron* according to claim 1, characterized in that: The soil improvement step S2 further includes the following steps: S1: pH adjustment: Adjust the pH value of the soil by applying an appropriate amount of lime or ferrous sulfate. S2: Organic matter addition: Add appropriate amounts of organic matter, such as compost and green manure, to increase the organic matter content of the soil.

4. The method for planting *Cistanche deserticola* by inoculating *Haloxylon ammodendron* on *Haloxylon ammodendron* according to claim 1, characterized in that: The inoculation technique in step S3 further includes the following steps: S1: Inoculation site selection: Select a healthy area near the roots of the Haloxylon ammodendron tree for inoculation to improve the success rate of inoculation; S2: Inoculation Depth Control: Control the inoculation depth to ensure contact between the seeds of Cistanche deserticola and the roots of Haloxylon ammodendron.

5. The method for planting *Cistanche deserticola* by inoculating *Haloxylon ammodendron* on *Haloxylon ammodendron* according to claim 1, characterized in that: The environmental control step S4 further includes the following steps: S1: Automated irrigation system: Automatically adjusts irrigation volume based on data from soil moisture sensors; S2: Intelligent Shading System: Automatically adjusts the opening and closing of the shading net based on data from light intensity and temperature sensors.

6. The method for planting *Cistanche deserticola* by inoculating *Haloxylon ammodendron* on *Haloxylon ammodendron* according to claim 1, characterized in that: Step S5, pest and disease control, specifically includes the following steps: S1: Biological pesticides: Using biological pesticides to replace chemical pesticides and reduce the impact on the environment; S2: Natural Enemy Release: Release natural enemy insects, such as ladybugs, to control pest populations.

7. The method for planting *Cistanche deserticola* by inoculating *Haloxylon ammodendron* on *Haloxylon ammodendron* according to claim 1, characterized in that: The harvesting and processing step S6 further includes the following steps: S1: Harvest time determination: The optimal harvest time is determined based on the growth cycle and medicinal component content of desert Cistanche deserticola; S2: Processing standardization: Establish standardized cleaning, drying and packaging processes to ensure product quality.