A composition containing an acetyl coenzyme A carboxylase inhibitor class of insecticide and acaricide

Abstract

The present application belongs to the field of insecticide and acaricide, and relates to a composition containing an acetyl-CoA carboxylase inhibitor insecticide and acaricide. The composition contains active component A and active component B, the weight ratio of the active component A to the active component B is 1:99-99:1; the active component A is selected from compound I, and the active component B is selected from an acetyl-CoA carboxylase inhibitor insecticide / acaricide; the structure of the compound I is as follows, and the composition has the advantages of obvious synergistic effect and resistance delay, and can be used for preventing and treating various pests.

Description

Technical Field

[0001] This invention belongs to the field of insecticides and acaricides, and relates to a composition containing an acetyl-CoA carboxylase inhibitor. Background Technology

[0002] In agricultural and horticultural crop production, damage caused by pests and diseases remains significant. Due to biodiversity and the simultaneous occurrence of multiple pests and diseases, the field of plant protection has a continuous need to develop new insecticides and acaricides with better activity, lower dosage, and greater environmental friendliness. Continuous use of insecticides and acaricides containing a single active ingredient in pest control easily leads to pesticide resistance in pests. Blending insecticides and acaricides with two or more active ingredients into mixed formulations plays an important role in broadening the insecticidal spectrum, controlling both pests and diseases, controlling viral diseases by controlling vector insects, reducing pesticide application costs for farmers, and delaying the development of pesticide resistance in pests. Summary of the Invention

[0003] The purpose of this invention is to provide a composition containing an acetyl-CoA carboxylase inhibitor to meet the increasing demand for insecticides and acaricides in the fields of agricultural and forestry and urban sanitation for pest control, which requires increasingly higher efficacy and more constantly updated varieties.

[0004] The technical solution of the present invention is as follows: an insecticidal and acaricidal composition, comprising active component A and active component B, wherein the weight ratio of active component A to active component B is 1:99 to 99:1; active component A is selected from compound I, and active component B is selected from acetyl-CoA carboxylase inhibitor insecticides / acaricides; the structure of compound I is as follows:

[0005]

[0006] In some embodiments, the active ingredient B is selected from one or more of spirodiclofen, spirotetramat, or spirodiclofen.

[0007] In some embodiments, active component A is compound I, and active component B is selected from one or more of spirodiclofen, spirotetramat, or spirodiclofen; the weight ratio of active component A to active component B is 50:1 to 1:50.

[0008] In some embodiments, the active component A is compound I, and the active component B is selected from one or more of spirodiclofen, spirotetramat, or spirodiclofen; the weight ratio of active component A to active component B is 50:1 to 1:20.

[0009] In some embodiments, the active component A is compound I, and the active component B is selected from one or more of spirodiclofen, spirotetramat, or spirodiclofen; the weight ratio between active component A and active component B is 10:1 to 1:20.

[0010] In some embodiments, active component A is compound I, and active component B is selected from one or more of spirodiclofen, spirotetramat, or spirodiclofen; the weight ratio of active component A to active component B is 1:1 to 1:20.

[0011] In some embodiments, the composition is mixed with a carrier and any adjuvant to form a formulation containing the composition.

[0012] The cumulative content of the active ingredient in the formulation is between 0.5% and 95%. Preferably, the cumulative content of the active ingredient in the formulation is between 1% and 85%.

[0013] A composition containing an acetyl-CoA carboxylase inhibitor is disclosed, the composition being used to prepare an insecticide / acaricide for controlling agricultural pests or urban sanitation pests.

[0014] The composition is applied to the pest that needs to be controlled or the medium in which it grows, in an effective dose.

[0015] The above description is specifically suitable for controlling a variety of important agricultural pests such as aphids, whiteflies, thrips, pear psyllids, scale insects, carmine spider mites, hawthorn spider mites, citrus psyllids, citrus rust mites, apple spider mites, two-spotted spider mites, gall mites, and flour mites, as well as urban pests such as termites, cockroaches, ants, flies, and mosquitoes. It can be used on fruit trees such as apples, pears, citrus, and lychees; cereals such as wheat and rice; legumes such as soybeans and kidney beans; cotton; vegetables such as cabbage, cauliflower, Chinese cabbage, rapeseed, tomatoes, and peppers; and flowers. When used to control urban pests, it can be applied in homes, various public places, offices, and on trees.

[0016] Furthermore, the method of using compositions containing acetyl-CoA carboxylase inhibitors for insecticides and acaricides involves applying the prepared composition, in effective doses, to the pests to be controlled or their growth media. A commonly chosen effective dose is typically 10 to 500 grams per hectare, with a preferred effective dose of 15 to 100 grams per hectare.

[0017] The present invention has the following advantages:

[0018] 1. The composition of the present invention exhibits a significant synergistic effect on insecticidal and acaricidal activity within a certain ratio range, thereby improving the control effect of the single active ingredient compound in the composition on pests.

[0019] 2. The dosage of the composition of the present invention when applied to kill insects and mites is significantly lower than that of a single compound in the composition when used to reduce the dosage of the compound, thereby reducing the cost of use and reducing environmental pollution.

[0020] 3. The active ingredients in the composition of the present invention have different mechanisms of action in killing insects and mites, and there is no problem of cross-resistance. Using it to control pests can delay the occurrence of pesticide resistance and improve the control effect on resistant species. Detailed Implementation

[0021] The following embodiments are intended to enable those skilled in the art to more fully understand the present invention, but are not intended to limit the invention to the scope of the embodiments described.

[0022] Indoor bioactivity assay

[0023] Example 1: Determination of the synergistic effect of the composition containing compound I on Tetranychus cinnabarinus eggs.

[0024] Test subject: Tetranychus cinnabarinus Boisduval, mite eggs, sensitive strains kept indoors, eggs laid 1 day ago.

[0025] Test conditions: Temperature: 24–28℃, Relative humidity: 30–60%, Light intensity: L:D = 12:12

[0026] Drug solution preparation: According to different test requirements, the test samples (different ratio compositions and original drugs of this invention) are accurately weighed using an electronic analytical balance. The samples are dissolved in acetone to prepare a mother liquor, and then diluted with 0.1% Tween-80 according to the experimental design dosage to prepare a drug solution with a certain concentration.

[0027] Experimental Method: Kidney bean seedlings at the first pair of true leaves unfolding stage were selected, leaving one true leaf intact. Ten female adult spider mites of uniform development were transferred to the leaf. After 24 hours, the female mites were removed, and the seedlings were sprayed with the solution. A blank control was treated first. The above procedure was repeated in ascending order of concentration, with three replicates for each treatment. After treatment, all mite eggs were transferred to an observation room. Seven days later, after all eggs in the blank control had hatched, the number of hatched eggs was counted, and the hatching inhibition rate was calculated.

[0028] The evaluation employed the Bliss method, a classic approach for evaluating the effects of mixtures. Based on his concept of independent synergistic effects, Bliss argued that the theoretical inhibition rate P of a mixture of insecticides and acaricides could be calculated using the following formula:

[0029] P = Pm + Pn(1 - Pm)

[0030] Pm represents the inhibition rate (%) of the target when the first active component is at a concentration of m; Pn represents the inhibition rate (%) of the target when the second active component is at a concentration of n.

[0031] If the actual inhibition rate of the target is greater than the theoretical inhibition rate P after the two active components are mixed at a certain concentration, it is determined that the two active components have a synergistic effect when used together at the set concentration; otherwise, they have an antagonistic effect.

[0032] Table 1. Synergistic effect determination of compound I with spirodiclofen and spirodiclofen on Tetranychus cinnabarin eggs.

[0033]

[0034]

[0035] Example 2: Indoor activity assay of the composition containing compound I against the eggs of Tetranychus cinnabarinus.

[0036] The synergistic effect data above show that the composition of compound I of the present invention mixed with spirodiclofen and spirodiclofen has a significant synergistic effect on adult Tetranychus cinnabarinus. Furthermore, the LC50 value and co-toxicity coefficient of the composition composed of compound I and spirodiclofen and spirodiclofen were determined.

[0037] Test subject: Tetranychus cinnabarinus Boisduval, mite eggs, sensitive strains kept indoors, eggs laid 1 day ago.

[0038] Test conditions: Temperature: 24–28℃, Relative humidity: 30–60%, Light intensity: L:D = 12:12

[0039] Drug solution preparation: According to different test requirements, the test samples (different ratio compositions and original drugs of this invention) are accurately weighed using an electronic analytical balance. The samples are dissolved in acetone to prepare a mother liquor, and then diluted with 0.1% Tween-80 according to the experimental design dosage to prepare a drug solution with a certain concentration.

[0040] Experimental Methods: Kidney bean seedlings at the first pair of true leaves unfolding stage were selected, leaving one true leaf. Ten female adult spider mites of uniform development were transferred to the leaf. After 24 hours, the female mites were removed, and the mixture was sprayed. A blank control was treated first. The above procedure was repeated in ascending order of concentration, with three replicates per treatment. After treatment, all mite eggs were transferred to the observation room. After 7 days, once all eggs in the blank control had hatched, the hatching number was recorded, and the hatching inhibition rate was calculated. Statistical analysis was performed using DPS data processing software to determine the toxicity regression equations, LC50 values, and 95% confidence limits for each tested single agent and each combination of different ratios. The co-toxicity coefficients of each ratio were calculated using the Sun YP method to evaluate the combined effects.

[0041] Table 2: Results of combined indoor toxicity assay of compound I and spirodiclofen against Tetranychus cinnabarin eggs

[0042]

[0043] Table 3: Results of combined in vitro toxicity assay of compound I and spirodiclofen against Tetranychus cinnabarin eggs

[0044]

[0045] The results showed that the co-toxicity coefficients of compound I with spirodiclofen and spirodiclofen were all greater than 120, indicating that compound I had a synergistic effect with spirodiclofen and spirodiclofen.

Claims

1. An insecticidal and acaricidal composition, comprising active component A and active component B, wherein the weight ratio of active component A to active component B is 1:99 to 99:1; active component A is selected from compound I, and active component B is selected from acetyl-CoA carboxylase inhibitor insecticides / acaricides; the structure of compound I is as follows:

2. The composition according to claim 1, characterized in that, The active ingredient B is selected from one or more of spirodiclofen, spirotetramat, or spirodiclofen.

3. The composition according to claim 1, characterized in that, The active component A is compound I, and the active component B is selected from one or more of spirodiclofen, spirotetramat, or spirodiclofen; the weight ratio of active component A to active component B is 50:1 to 1:

50.

4. The composition according to claim 1, characterized in that, The active component A is compound I, and the active component B is selected from one or more of spirodiclofen, spirotetramat, or spirodiclofen; the weight ratio of active component A to active component B is 50:1 to 1:

20.

5. The composition according to claim 1, characterized in that, The active component A is compound I, and the active component B is selected from one or more of spirodiclofen, spirotetramat, or spirodiclofen; the weight ratio of active component A to active component B is 10:1 to 1:

20.

6. The composition according to claim 1, characterized in that, The active component A is compound I, and the active component B is selected from one or more of spirodiclofen, spirotetramat, or spirodiclofen; the weight ratio of active component A to active component B is 1:1 to 1:

20.

7. The composition according to claim 1, characterized in that, The composition is mixed with a carrier and any adjuvant to prepare a formulation containing the composition of claim 1.

8. The composition according to claim 1, characterized in that, The cumulative content of the active ingredient in the formulation is between 0.5% and 95%, preferably between 1% and 85%.

9. The application of the insecticidal and acaricidal composition according to claim 1, characterized in that, The composition is used to prepare an insecticide and acaricide for controlling agricultural pests or urban sanitation pests.

10. The application of the insecticidal and acaricidal composition according to claim 9, characterized in that, The composition is applied to the pest that needs to be controlled or the medium in which it grows, in an effective dose.

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