A rodent wide-range high-low temperature controllable oral-nasal inhalation exposure experiment system

Abstract

The application discloses a kind of rodent wide range high and low temperature controllable oral-nasal inhalation exposure experimental system, belong to animal modeling technical field.This device integrates gas source pretreatment module, extremely low temperature refrigeration gas path module, high temperature heating gas path module, gas path switching valve group, temperature flow monitoring module, animal oral-nasal airtight fixing module and waste gas treatment module;Realize-40 ℃ stable low-temperature gas output through cryogenic heat exchange structure, realize room temperature to 80 ℃ high-temperature gas continuous adjustable through stainless steel electric heating and PID closed-loop temperature control, realize cold, hot, normal temperature gas path fast switching using solenoid valve group.Collaborate with the special split type airtight restraint fixer of big and small mouse, only the oral-nasal of animal is carried out directional cold-hot gas inhalation stimulation, body is isolated in conventional room temperature environment, eliminate whole body temperature interference.The application solves the technical defects that traditional equipment temperature zone is narrow, function is single, extremely low temperature output is insufficient, high temperature can cause animal damage, experimental variable is uncontrollable.

Description

Technical Field

[0001] This invention relates to the field of modeling system technology, and more specifically, to a wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system for rodents. Background Technology

[0002] Animal oral-nasal exposure experiments precisely expose laboratory animals to a controlled aerosol / gas environment through their mouth and nose, avoiding skin and hair contact. These experiments are specifically designed for toxicological, pharmacodynamic, and mechanistic studies via the respiratory route, making the results more closely resemble real-world human respiratory exposure scenarios. The core of existing devices for animal oral-nasal exposure experiments is primarily the oral-nasal exposure tower, which restrains the animal and simultaneously exposes its mouth and nose to the tested aerosol / gas environment.

[0003] However, existing equipment has limited functionality, only capable of operating at a single low temperature or a single high temperature, and cannot rapidly switch between extremely low temperature cold air, normal temperature gas, and high temperature hot gas on the same device. At the same time, existing equipment lacks a dedicated mouth and nose sealing air intake structure, making it easy for cold and hot gases to leak and diffuse, and unable to guarantee that only the respiratory tract receives cold and hot stimuli. Summary of the Invention

[0004] This invention provides a wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system for rodents, which solves the problem that existing equipment can only achieve single low temperature or single high temperature operation and lacks a dedicated oral and nasal sealing air intake structure.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system for rodents includes a gas source pretreatment module, a low-temperature refrigeration gas path module, a high-temperature heating gas path module, a gas path switching valve group, a temperature and flow monitoring module, an animal oral and nasal sealing and fixation module, and an exhaust gas treatment module. The gas source pretreatment module is connected to the inlet ends of both the low-temperature refrigeration gas path module and the high-temperature heating gas path module. The outlet ends of the low-temperature refrigeration gas path module and the high-temperature heating gas path module are connected to the gas path switching valve group. The output end of the gas path switching valve group is connected to the animal oral and nasal sealing and fixation module via the temperature and flow monitoring module. The exhalation end of the animal oral and nasal sealing and fixation module is connected to the exhaust gas treatment module.

[0007] Preferably, the low-temperature refrigeration gas circuit module adopts a cryogenic heat exchange structure, including an insulated cold storage container, a metal heat exchange coil, and a fully insulated delivery pipeline, which can stably produce controllable cold air at -40℃.

[0008] Preferably, the high-temperature heating gas circuit module includes a stainless steel gas heating tube, a PID temperature controller and a constant temperature buffer chamber. The output gas temperature range of the high-temperature heating gas circuit module is room temperature to 80°C, and it has an over-temperature power-off protection structure.

[0009] Preferably, the gas path switching valve group is a multi-way solenoid valve combination, which can switch between three working modes: low temperature gas path, high temperature gas path, and normal temperature control gas path.

[0010] Preferably, the animal mouth and nose sealing and fixing module includes a detachable mouse restraint tube and a rat restraint tube. The body of the mouse restraint tube and the rat restraint tube is a sealed limiting structure, and the front end of the body is provided with a sealed mouth and nose air inlet.

[0011] Preferably, the metal heat exchange coil is an oxygen-free copper extended coil, which is completely immersed in the low-temperature heat exchange medium, and the outer wall of the conveying pipeline is covered with a heat insulation layer.

[0012] Preferably, the high-temperature heating gas path module is equipped with a probe for real-time acquisition of the outlet temperature.

[0013] Preferably, the temperature and flow monitoring module is equipped with a multi-channel adjustable flow meter, and the flow rate adjustment range of the adjustable flow meter is 0.3 to 5 L / min.

[0014] Preferably, the air source pretreatment module includes an oil-free air compressor, a drying component, and a multi-stage filtration component.

[0015] Preferably, the waste gas treatment module adopts a negative pressure collection and adsorption filtration structure, and the filtration structure includes a detachable filter screen.

[0016] The principle and beneficial effects of this technical solution:

[0017] (1) The present invention adopts a wide temperature range integrated design, which integrates the ultra-low temperature refrigeration module and the high temperature heating module through the same gas circuit system, stably covering the ultra-large temperature range of -40℃ to 80℃, realizing one-click switching of cold gas, hot gas and normal temperature gas modes, breaking the technical limitations of traditional equipment that only cools and only heats, and is multi-functional with high integration.

[0018] (2) The present invention adopts a -40℃ extremely low temperature stable gas supply structure, and adopts a deep cryogenic heat exchange with an immersion metal heat exchange coil and a fully sealed heat-insulated gas path design, which effectively solves the problems of temperature rise, pipeline frost, ice blockage during the low temperature gas transportation process, and ensures continuous and stable output of -40℃ cold air with temperature fluctuation ≤±1℃.

[0019] (3) The present invention adopts high temperature buffer constant temperature and multiple anti-scalding protection. The pipe electric heating is combined with PID closed loop precise temperature control and a high temperature buffer pressure stabilizing chamber is added to avoid the impact of instantaneous high temperature airflow. Combined with the real-time temperature monitoring of the air outlet and the automatic power-off protection structure for over-temperature, it effectively prevents animal respiratory tract burns and improves experimental safety.

[0020] (4) The present invention adopts a split airtight mouth and nose independent fixation unit, and designs a replaceable mouse and rat special airtight restraint tube. The front end is set with a sealed mouth and nose air inlet interface, and adopts a one-way air intake and independent waste discharge structure; strictly isolates the animal trunk from the extreme temperature airflow, realizes pure respiratory targeted stimulation, and the experimental variables are precise and controllable.

[0021] (5) The present invention adopts multi-parameter collaborative intelligent control. By integrating air drying and filtration pretreatment, high-precision adjustable flow meter, electromagnetic valve automatic switching, dual-point temperature real-time monitoring, and negative pressure exhaust gas collection and treatment system, it can adapt to different respiratory flow requirements of mice and rats, support multi-channel parallel expansion, and has a high degree of standardization. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0023] Figure 2 This is a schematic diagram of the composition of the gas source pretreatment module;

[0024] Figure 3 This is a schematic diagram of the composition and structure of the low-temperature refrigeration gas circuit module;

[0025] Figure 4 This is a schematic diagram of the composition and structure of the high-temperature heating gas circuit module;

[0026] Figure 5 A schematic diagram of the composition of the animal mouth and nose sealing and fixation module and the exhaust gas treatment module; Detailed Implementation

[0027] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments:

[0028] Example:

[0029] This device mainly includes: a gas source pretreatment module, a low-temperature refrigeration gas circuit module, a high-temperature heating gas circuit module, a gas circuit switching control module, a temperature and flow monitoring module, an animal mouth and nose sealing and fixing module, a safety protection module, and an exhaust gas treatment module.

[0030] Specific structure and function of each module

[0031] (1) Gas source pretreatment module

[0032] It consists of an oil-free air compressor, an air drying component, and a multi-stage dust filter component; it can effectively remove moisture, dust and impurities from compressed air, prevent pipeline freezing and blockage in low-temperature environments and carbon buildup and oxidation in high-temperature environments, and ensure that the intake air is clean and dry.

[0033] (2) Low-temperature refrigeration gas circuit module

[0034] It adopts a cryogenic immersion heat exchange structure, including an insulated cold storage container, an extended oxygen-free copper heat exchange coil, and pressure-resistant insulated delivery pipelines; the heat exchange coil is completely immersed in the low-temperature heat exchange medium, and dry compressed air flows through the coil to complete heat exchange, stably producing -40℃ gradient cold air; all low-temperature delivery pipelines are covered with a thickened heat insulation layer to reduce cold loss and prevent condensation and frost on the outer wall of the pipeline.

[0035] (3) High-temperature heating gas circuit module

[0036] It consists of 304 stainless steel explosion-proof heating pipes, PID intelligent temperature controller, and constant temperature buffer and pressure stabilizing chamber; the electric heating module has independent closed-loop temperature control, and the outlet air temperature is continuously adjustable in the range of room temperature to 80℃; the buffer chamber balances the airflow temperature to avoid local overheating; the system has a preset temperature safety threshold, and automatically cuts off power and provides audible and visual alarms when the temperature exceeds the limit, eliminating the risk of dry burning and high temperature burns.

[0037] (4) Gas path switching control module

[0038] It adopts a multi-way two-position three-way solenoid valve group to control the opening and closing of the low temperature gas path, high temperature gas path and normal temperature control gas path; it supports two modes: manual switching and timed automatic switching, which can quickly realize the flexible switching of extreme cold inhalation, high temperature inhalation and blank control experimental modes.

[0039] (5) Animal mouth and nose sealing and fixing module

[0040] It includes detachable and adjustable mouse restraint tubes and rat restraint tubes. The tube body has a sealed limiting structure and a locking and fixing structure at the tail. The front end of the tube body is equipped with a sealed mask-type air inlet, which only connects to the animal's mouth and nose area to ensure the directional inhalation of hot and cold gases. The animal's torso is completely placed in a normal temperature experimental environment to completely eliminate the interference of whole-body temperature.

[0041] (6) Temperature and flow monitoring module

[0042] Each airway is equipped with an independent high-precision flow meter with a single-channel flow rate adjustment range of 0.3–5 L / min, which can match the respiratory gas supply needs of mice (0.5–1 L / min) and rats (1.5–3 L / min). Dual temperature sensors are installed at the end of the airway and the inlet at the mouth and nose to display temperature data in real time and achieve closed-loop precise temperature control.

[0043] (7) Safety protection and exhaust gas treatment module

[0044] Equipped with low-temperature antifreeze warning, high-temperature overheating power-off, and pipeline pressure overload protection devices; animal exhaled waste gas is collected centrally under negative pressure, purified by activated carbon adsorption and filtration, and then uniformly discharged, effectively preventing the spread of bioaerosols and ensuring the safety of the experimental environment.

[0045] Device Working Process

[0046] (1) Pre-experiment preparation: Select low temperature mode, high temperature mode or normal temperature control mode according to the experimental plan, set the target temperature and gas flow rate, pre-cool or preheat the equipment in advance, and start the experiment after the gas temperature stabilizes.

[0047] (2) Animal fixation: Place the experimental mice or rats inside the corresponding size of the sealed restraint tube, adjust the limiting structure to complete the fixation, and connect the nose seal air inlet at the front port to ensure airtightness.

[0048] (3) Cold and hot gas supply: The air compressor outputs clean and dry air, which is pre-treated and then introduced into the low-temperature refrigeration gas path or the high-temperature heating gas path through the switching valve, and adjusted to the set target temperature and flow rate.

[0049] (4) Directed inhalation exposure: Constant temperature hot and cold gas is stably delivered to the mouth and nose of the animal, and the respiratory tract is stimulated by directed inhalation according to the experimental set time.

[0050] (5) Post-processing: After the experiment, turn off the heating or cooling module, purge the gas path with room temperature air, remove the animal fixation device, purify the waste gas, and reset the equipment for standby.

[0051] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solutions of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system for rodents, characterized in that: The system includes a gas source pretreatment module, a low-temperature refrigeration gas path module, a high-temperature heating gas path module, a gas path switching valve group, a temperature and flow monitoring module, an animal mouth and nose sealing and fixation module, and an exhaust gas treatment module. The gas source pretreatment module is connected to the inlet ends of both the low-temperature refrigeration gas path module and the high-temperature heating gas path module. The outlet ends of the low-temperature refrigeration gas path module and the high-temperature heating gas path module are connected to the gas path switching valve group. The output end of the gas path switching valve group is connected to the animal mouth and nose sealing and fixation module via the temperature and flow monitoring module. The exhalation end of the animal mouth and nose sealing and fixation module is connected to the exhaust gas treatment module.

2. The rodent wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system according to claim 1, characterized in that: The low-temperature refrigeration gas circuit module adopts a cryogenic heat exchange structure, including an insulated cold storage container, a metal heat exchange coil, and a fully insulated delivery pipeline, which can stably produce controllable cold air at -40℃.

3. The rodent wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system according to claim 2, characterized in that: The high-temperature heating gas circuit module includes a stainless steel gas heating tube, a PID temperature controller, and a constant temperature buffer chamber. The output gas temperature range of the high-temperature heating gas circuit module is room temperature to 80°C, and it has an over-temperature power-off protection structure.

4. The rodent wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system according to claim 3, characterized in that: The gas path switching valve group is a multi-way solenoid valve combination, which can switch between three working modes: low temperature gas path, high temperature gas path, and normal temperature control gas path.

5. The rodent wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system according to claim 4, characterized in that: The animal mouth and nose sealing and fixation module includes detachable mouse restraint tubes and rat restraint tubes. The bodies of the mouse restraint tubes and rat restraint tubes are sealed and limiting structures, and the front end of the body is provided with a sealed mouth and nose air inlet.

6. The rodent wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system according to claim 5, characterized in that: The metal heat exchange coil is made of oxygen-free copper with an extended coil, and is completely immersed in the low-temperature heat exchange medium. The outer wall of the conveying pipeline is covered with a heat insulation layer.

7. The rodent wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system according to claim 6, characterized in that: The high-temperature heating gas path module is equipped with a probe for real-time acquisition of the outlet temperature.

8. The rodent wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system according to claim 7, characterized in that: The temperature and flow monitoring module is equipped with a multi-channel adjustable flow meter, and the flow rate adjustment range of the adjustable flow meter is 0.3 to 5 L / min.

9. The rodent wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system according to claim 8, characterized in that: The air source pretreatment module includes an oil-free air compressor, a drying component, and a multi-stage filtration component.

10. The rodent wide-range high and low temperature controllable oral and nasal inhalation exposure experimental system according to claim 9, characterized in that: The waste gas treatment module adopts a negative pressure collection and adsorption filtration structure, and the filtration structure includes a detachable filter screen.

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