Compositions, personal care products and uses thereof

Abstract

The application provides a composition comprising salicin, olea europaea fruit extract and glucosyl rutin-containing raw material; the mass ratio of the salicin, the olea europaea fruit extract and the glucosyl rutin-containing raw material is 0.5-5:0.05-0.5:0.01-0.5. The application provides use of the above-mentioned composition in preparation of barrier repair, antioxidant, anti-aging and / or whitening products. The application also provides use of salicin in preparation of barrier repair and / or anti-aging products. The application also provides a personal care product comprising the composition described in the above technical solution. The application compounds salicin, olea europaea fruit extract and glucosyl rutin (or glucosyl rutin-containing raw material), which can significantly improve antioxidant effect, has a synergistic antioxidant effect, can be used in preparation of products for preventing or repairing skin problems caused by oxidative stress damage, and can also resist free radical system by thickening the epidermal layer and construct a photoprotective barrier.

Description

Technical Field

[0001] This application relates to the field of daily chemical products technology, and in particular to a composition, personal care product and its application. Background Technology

[0002] The balance between oxidation and antioxidation is crucial for cellular health and is extremely important for overall human health. Many life processes, such as respiration, nutrient metabolism, and detoxification, involve oxidation, producing free radicals and reactive oxygen species that play roles such as signal transduction and infection resistance. However, research has found that excessive free radicals in the body cause oxidation reactions in various substances that make up cells, such as carbohydrates, DNA, proteins, and lipids, leading to denaturation, cross-linking, and breakage, damaging normal cells and accelerating cellular aging.

[0003] As people's understanding of skin antioxidants increases, natural antioxidants are being incorporated into skincare products to enhance the skin's antioxidant capacity. However, currently available natural antioxidants have relatively low scavenging rates against free radicals, especially DPPH free radicals. Summary of the Invention

[0004] In view of this, the purpose of this application is to provide an anti-oxidant composition, personal care product and its application. The composition provided by this application has a high scavenging rate of DPPH free radicals and has a synergistic antioxidant effect, while also having the effects of barrier repair, anti-aging and whitening.

[0005] This application provides a composition comprising salicin, olive fruit extract and glucosylrutin raw material; The mass ratio of salicin, olive fruit extract and glucosylrutin raw material is 0.5~5:0.05~0.5:0.01~0.5.

[0006] The composition provided in this application includes salicin, also known as salicin or salicylose, which is a phenolic glycoside compound with the chemical formula C. 13 H 18 O7 possesses anti-inflammatory, analgesic, and antioxidant bioactivities. The applicant discovered that salicin can significantly enhance the expression of barrier repair-related genes, such as lobelin (LOR), filaggrin (FLG), and glutaminase 1 (TGM1), thereby effectively thickening the epidermis and also thickening the dermis, thus making it suitable for barrier repair and / or anti-aging. The applicant also found that the combination of salicin, olive fruit extract, and glucosylrutin significantly enhances antioxidant activity, exhibiting a synergistic antioxidant effect. This can be used to prepare products for preventing or repairing skin problems caused by oxidative stress, and can also construct a photoprotective barrier by thickening the epidermis—a system that resists free radicals. This application does not impose any special restrictions on the source of the salicin; it can be purchased commercially.

[0007] The composition provided in this application includes olive fruit extract, which is an extract of olive fruit and has antioxidant and anti-inflammatory effects. This application does not impose any particular limitation on the olive fruit extract, but preferably it is an ethyl acetate extract of olive fruit, which can be purchased commercially or extracted according to the method disclosed in CN113277931A. In some specific implementations, the olive fruit extract contains olive polyphenols. Preferably, the olive polyphenols include hydroxytyrosol. In some specific implementations, the olive fruit extract includes 20 wt% or more, preferably 20 wt% to 60 wt%, more preferably 35 wt% to 45 wt% of hydroxytyrosol.

[0008] The composition provided in this application includes glucosylrutin or a glucosylrutin-containing raw material. Glucosylrutin has the following structure and possesses effects such as UV protection, blue light protection, free radical scavenging, and antioxidant properties: .

[0009] This application does not impose any special restrictions on the source of the glucosylrutin; it can be purchased from the market.

[0010] When the composition includes a glucosylrutin-containing raw material, the glucosylrutin content in the raw material is 50 wt% or more. In some specific implementations, the glucosylrutin-containing raw material comprises 50 wt% to 100 wt% glucosylrutin, 0 wt% to 30 wt% isoquercitrin, and the balance being water. In some specific implementations, the glucosylrutin-containing raw material comprises 60 wt% to 100 wt% glucosylrutin, 0 wt% to 20 wt% isoquercitrin, and the balance being water. In some specific implementations, the glucosylrutin-containing raw material comprises 70 wt% glucosylrutin, 20 wt% isoquercitrin, and the balance being water. This application does not impose any special restrictions on the source of the glucosylrutin-containing raw material; commercially available products are acceptable, such as the product with trade name AC-GR-01.

[0011] In some specific implementations, the mass ratio of salicin, olive fruit extract and glucosylrutin-containing raw material is 0.8~3:0.2~0.35:0.1~0.3, for example, 0.8:0.2:0.3, 1:0.25:0.25, 1.5:0.3:0.2 or 3:0.35:0.1.

[0012] This application combines salicin, olive fruit extract, and glucosylrutin (or raw materials containing glucosylrutin) to significantly enhance antioxidant activity and achieve synergistic antioxidant effects. It can be used to prepare products for preventing or repairing skin problems caused by oxidative stress. It can also build a photoprotective barrier by thickening the epidermis to resist free radicals. In addition, it has the effects of barrier repair, anti-aging, and whitening.

[0013] Based on this, this application provides the use of the above composition in the preparation of barrier repair, antioxidant, anti-aging and / or whitening products. In some specific implementations, the antioxidant is the scavenging of free radicals, such as scavenging DPPH free radicals.

[0014] As described above, the applicant discovered that salicin can significantly enhance the expression of barrier repair-related genes, such as lobelin (LOR), filaggrin (FLG), and glutamine transferase 1 (TGM1), thereby effectively thickening the epidermis and also thickening the dermis, thus making it suitable for barrier repair and / or anti-aging. Based on this, this application also provides the use of salicin in the preparation of barrier repair and / or anti-aging products. In some specific implementations, the salicin can enhance the expression of at least one of lobelin, filaggrin, and glutamine transferase 1; preferably, the salicin can enhance the expression of lobelin, filaggrin, and glutamine transferase 1. In some specific implementations, the salicin can also increase the thickness of the skin surface layer and / or the thickness of skin collagen.

[0015] This application also provides a personal care product comprising the composition described in the above technical solution.

[0016] In some embodiments of this application, the aforementioned products include, but are not limited to, personal care products and pharmaceuticals. Those skilled in the art will understand that, in addition to the compositions described in the above technical solutions, other excipients are also included, such as pharmaceutical excipients or excipients for personal care products.

[0017] In some embodiments of this application, the personal care products include: basic care products, hair care products and / or makeup products.

[0018] In some embodiments of this application, the basic care product includes, but is not limited to: Facial cleanser / cleanser / facial lotion: Gently removes dirt, oil, and makeup residue from the face; Cleansing oil / cleansing water / cleansing balm: specially designed to thoroughly remove makeup, especially waterproof cosmetics; Toner / lotion: Used after cleansing, it can further cleanse the skin's surface of residue, while replenishing the skin's moisture, restoring the skin's pH balance, and laying a good foundation for the absorption of subsequent skin care products; Serum: Contains a high concentration of active ingredients, providing deep nourishment and repair for specific skin problems (such as anti-aging, moisturizing, whitening, etc.); Eye cream: Specially designed for the sensitive area around the eyes, it has the effects of reducing fine lines and dark circles and firming the skin around the eyes. The texture is usually light and easily absorbed. Day / night lotion or cream: It has functions such as protection, repair and nourishment, and promotes cell regeneration. It can provide the skin with the necessary moisture and lock in the replenished moisture. Sunscreen: Used for ultraviolet protection and prevention of photoaging, etc. Face masks: provide extra nourishment and care for the skin, such as hydration, pore cleansing, or brightening of the complexion.

[0019] In some embodiments of the present invention, the washing and care products include, but are not limited to: Scalp cleansing and care products: mainly used to clean the scalp and hair, and provide repair and moisturizing effects, including shampoos, hair creams and other cleansing products or conditioners, hair masks, hair oils and other care products; Body cleansing and care: Cleansing and moisturizing of the entire body's skin to maintain healthy skin condition, including cleansing products such as shower gel, soap, and bath oil, or care products such as body lotion, moisturizer, and scrub; Hand and other body part cleaning and care products: These are products for local cleaning and care, including hand sanitizers and other cleaning products, or hand creams and other care products.

[0020] In some embodiments of this application, the makeup products include, but are not limited to: (1) Base makeup: Foundation / cream: Used to even out skin tone and cover blemishes; BB cream / CC cream: A lightweight base makeup product that combines skincare and makeup application; Concealer / pen: Used to cover up specific areas such as pimples and dark circles; Loose powder / setting powder: Sets makeup and reduces facial shine.

[0021] (2) Cheek makeup: Blush: Adds natural color to the cheeks and improves complexion; Contouring powder / stick: Use shading techniques to make facial contours more three-dimensional; (3) Lip makeup: Lipstick / lip gloss / lip tint: to change or emphasize the color of the lips; Lip liner: Defines the clear outline of the lips and prevents lipstick from bleeding.

[0022] (4) Multifunctional cosmetics: Highlighter sticks / liquids / powders: Highlight high points on the face (such as the bridge of the nose and cheekbones) to create a luminous effect.

[0023] In addition, there are products designed specifically for special occasions, such as long-lasting, non-fading lipsticks.

[0024] In some specific implementations, the composition described in the above technical solution is preferably mixed with a cosmetically acceptable carrier to prepare products including shampoo, shower gel, facial cleanser, serum, lotion, cream, gel, or mask, which can be used to prevent or repair skin problems caused by oxidative stress or to build a photoprotective barrier by thickening the epidermis to resist free radicals. It also has the effects of barrier repair, anti-aging, and whitening.

[0025] In some specific implementations, the personal care product includes 0.05wt% to 10wt% of the composition described in the above technical solution, preferably 0.1wt% to 9.5wt% of the composition described in the above technical solution, and more preferably 0.5wt% to 9wt% of the composition described in the above technical solution.

[0026] This application combines salicin, olive fruit extract, and glucosylrutin (or raw materials containing glucosylrutin) to significantly enhance antioxidant activity and achieve synergistic antioxidant effects. It can be used to prepare products for preventing or repairing skin problems caused by oxidative stress. It can also build a photoprotective barrier by thickening the epidermis to resist free radicals, and has the effects of barrier repair, anti-aging, and whitening. Attached Figure Description

[0027] Figure 1 The cell viability curve of salicin in the sample; Figure 2 This is a cell morphology image of the salicin sample. Detailed Implementation

[0028] It should be understood that the expression “one or more of…” individually includes each of the objects described after the expression, as well as various different combinations of two or more of the described objects, unless otherwise understood from the context and usage. The expression “and / or” combined with three or more described objects should be understood to have the same meaning, unless otherwise understood from the context.

[0029] The terms “including,” “having,” or “containing,” including the use of their grammatical synonyms, should generally be understood as open-ended and non-restrictive, for example, not excluding other unstated elements or steps, unless otherwise specifically stated or understood from the context.

[0030] It should be understood that the order of steps or the sequence of actions is not important as long as this application remains operational. Furthermore, two or more steps or actions may be performed simultaneously.

[0031] This application provides a composition comprising salicin, olive fruit extract and glucosylrutin raw material; The mass ratio of salicin, olive fruit extract and glucosylrutin raw material is 0.5~5:0.05~0.5:0.01~0.5.

[0032] In some specific implementations, the mass ratio of salicin, olive fruit extract and glucosylrutin-containing raw material is 0.8~3:0.2~0.35:0.1~0.3, for example, 0.8:0.2:0.3, 1:0.25:0.25, 1.5:0.3:0.2 or 3:0.35:0.1.

[0033] This application combines salicin, olive fruit extract, and glucosylrutin (or raw materials containing glucosylrutin) to significantly enhance antioxidant activity and achieve synergistic antioxidant effects. It can be used to prepare products for preventing or repairing skin problems caused by oxidative stress. It can also build a photoprotective barrier by thickening the epidermis to resist free radicals, and has the effects of barrier repair, anti-aging, and whitening.

[0034] Based on this, this application provides the use of the above composition in the preparation of barrier repair, antioxidant, anti-aging and / or whitening products. In some specific implementations, the antioxidant is the scavenging of free radicals, such as scavenging DPPH free radicals.

[0035] As described above, the applicant discovered that salicin can significantly enhance the expression of barrier repair-related genes, such as lobelin (LOR), filaggrin (FLG), and glutamine transferase 1 (TGM1), thereby effectively thickening the epidermis and also thickening the dermis, thus making it suitable for barrier repair and / or anti-aging. Based on this, this application also provides the use of salicin in the preparation of barrier repair and / or anti-aging products. In some specific implementations, the salicin can enhance the expression of at least one of lobelin, filaggrin, and glutamine transferase 1; preferably, the salicin can enhance the expression of lobelin, filaggrin, and glutamine transferase 1. In some specific implementations, the salicin can also increase the thickness of the skin surface layer and / or the thickness of skin collagen.

[0036] This application also provides a personal care product comprising the composition described in the above technical solution.

[0037] In some embodiments of this application, the aforementioned products include, but are not limited to, personal care products and pharmaceuticals. Those skilled in the art will understand that, in addition to the compositions described in the above technical solutions, other excipients are also included, such as pharmaceutical excipients or excipients for personal care products.

[0038] In some embodiments of this application, the personal care products include: basic care products, hair care products and / or makeup products.

[0039] This application combines salicin, olive fruit extract, and glucosylrutin (or raw materials containing glucosylrutin) to significantly enhance antioxidant activity and achieve synergistic antioxidant effects. It can be used to prepare products for preventing or repairing skin problems caused by oxidative stress. It can also build a photoprotective barrier by thickening the epidermis to resist free radicals, and has the effects of barrier repair, anti-aging, and whitening.

[0040] The present application is further illustrated below with reference to embodiments. The scope of protection of the present application is not limited to the following embodiments.

[0041] In the following examples, the purity of salicin is ≥98%; Olive fruit extract is an ethyl acetate extract of olive fruit, with a hydroxytyrosol content of approximately 40%. Olive leaf extract is an aqueous extract of olive leaves, containing approximately 40% oleuropein. The commercial brand name for glucosylrutin is AC-GR-01, which consists of 70% glucosylrutin, 20% isoquercitrin and 10% water.

[0042] Experimental Example 1: Cytotoxicity Test

[0043] 1.1 Cell viability testing methods

[0044] (1) Cell seeding: After reviving keratinocytes, when the plating rate reaches about 60%, the cells are seeded into 96-well plates and incubated overnight in a CO2 incubator (37°C, 5% CO2).

[0045] (2) Experimental grouping: The experiment was set up with a zeroing group, a solvent control group, a positive control group and a sample group. In the sample group, each sample was set with 8 concentrations, and 3 replicate wells were set for each concentration.

[0046] (3) Solution preparation: Prepare working solutions of different concentrations according to the test concentration gradient table shown in Table 1.

[0047] Table 1 Test Concentration Setting Table

[0048] (4) Drug administration: Drug administration was performed when the cell seeding rate in the 96-well plate reached 50%~60%. For the solvent control group (Solvent Control), 200 μL of KcGrowth culture medium (Guangdong Boxi Shaanxi Branch) was added to each well; for the positive control group, 200 μL of 10% DMSO culture medium was added to each well; for the sample group, 200 μL of culture medium containing the corresponding concentration of the sample was added to each well; for the zeroing group, no cells were seeded, only 200 μL of KcGrowth culture medium was added. After drug administration, the 96-well plate was placed in a CO2 incubator (37℃, 5% CO2) and incubated for 24 h.

[0049] (5) Detection: After culturing cells for 24 hours, discard the supernatant, add MTT working solution (0.5 mg / mL), and incubate at 37°C in the dark for 4 hours. After incubation, discard the supernatant, add 150 µL of DMSO to each well, and read the OD value at 490 nm.

[0050] (6) Calculation of relative cell viability: Calculate the relative cell viability according to the formula: .

[0051] 1.2 Cell morphology testing methods

[0052] (1) Cell seeding: After reviving keratinocytes, when the plating rate reaches about 60%, seed the cells into 24-well plates and incubate overnight in a CO2 incubator (37℃, 5% CO2).

[0053] (2) Experimental grouping: The experiment set up a solvent control group and a sample group. In the sample group, each sample was set with 5 concentration gradients, namely 1%, 0.5%, 0.25%, 0.125% and 0.0625%.

[0054] (3) Drug administration: Drug administration was carried out when the cell deposition rate in the 24-well plate reached 50%~60%. 1 mL of culture medium was added to each well of the solvent control group; 1 mL of culture medium containing the corresponding concentration of the test sample was added to each well of the sample group. After drug administration, the 24-well plate was placed in a CO2 incubator (37℃, 5% CO2) and cultured for 24 h.

[0055] (4) Taking pictures: After incubation, discard the supernatant and take pictures under an inverted microscope.

[0056] 1.3 Test Results

[0057] The results are shown in Table 2. Figure 1 and Figure 2 Table 2 shows the MTT assay results for salicin in the samples. Figure 1 The graph shows the cell viability curve of salicin in the sample. Figure 2 This is a cell morphology image of the salicin sample.

[0058] Table 2. MTT assay results of salicin in the samples.

[0059] From Table 2, Figure 1 and Figure 2 It is known that salicin is essentially non-toxic to cells at a concentration of 0.5%, and will not affect cell viability or cell morphology.

[0060] Experimental Example 2: Based on a 3D Epidermal Skin Model (EpiKutis) ® The expression levels of lobe protein (LOR), filaggrin (FLG), and glutamine transferase 1 (TGM1) were tested.

[0061] 2.1 Test Method

[0062] (1) Test group

[0063] Grouped according to the concentrations shown in Table 3: Table 3 Test Groups

[0064] (2) Administration

[0065] a. According to the test group, transfer the detection model to a 6-well plate (add 0.9 mL of EpiGrowth culture medium in advance), and label the test group number on the 6-well plate.

[0066] b. All PC group and sample group were administered the drug via surface treatment. After administration, they were incubated in a CO2 incubator (37℃, 5% CO2) for 24 hours.

[0067] c. After incubation, the test model was rubbed for 2 minutes and washed twice with 250 μL of cleaning solution (0.1% Triton X-100) each time. The cleaning solution was collected and kept for later use. The model was then fixed in 4% paraformaldehyde solution.

[0068] (3) Immunofluorescence assay

[0069] The model used for testing was fixed with 4% paraformaldehyde for 24 hours. Immunofluorescence detection was performed, and the images were photographed and analyzed under a microscope.

[0070] (4) Calculation of lift rate:

[0071] (5) Statistical analysis of results

[0072] GraphPad Prism was used for plotting, and the results are expressed as Mean ± SD. t-tests were used for comparisons between groups. All statistical analyses were two-tailed. P < 0.05 was considered statistically significant, and P < 0.01 was considered highly statistically significant.

[0073] 2.2 Test Results

[0074] The test results are shown in Tables 4, 5 and 6.

[0075] Table 4 Summary of LOR Immunofluorescence Analysis Results

[0076] Note: The average integrated optical density (IOD) reflects the content of LOR. When performing statistical analysis using the t-test method, the significance compared to group BC is... This means that a p-value < 0.05 indicates that... A p-value < 0.01 indicates that... .

[0077] Table 5 Summary of FLG Immunofluorescence Analysis Results

[0078] Note: The average integrated optical density (IOD) reflects the FLG content. When performing statistical analysis using the t-test method, the significance level compared to group BC is... This means that a p-value < 0.05 indicates that... A p-value < 0.01 indicates that... .

[0079] Table 6 Summary of TGM1 Immunofluorescence Analysis Results

[0080] Note: The average integrated optical density (IOD) reflects the TGM1 content. When performing statistical analysis using the t-test method, the significance level compared to group BC is... This means that a p-value < 0.05 indicates that... A p-value < 0.01 indicates that... .

[0081] As shown in Tables 4-6, the contents of lobe protein (LOR), filaggrin (FLG), and glutamin transferase 1 (TGM1) in the samples were significantly increased at concentrations of salicin of 0.3% (m / m) and 2% (m / m).

[0082] Experimental Example 3: Thickening of the skin barrier in zebrafish

[0083] 3.1 Test Method: Three 12-month-old male zebrafish were randomly selected and exposed to a 0.015 g / L salicin solution, with a blank control group included. The solution was changed every other day. On the 7th day of exposure, the fish were anesthetized with tricaine, and a small piece of skin tissue was collected from the same body part, fixed, paraffin-embedded, stained with H&E, and then the epidermal thickness at five points in each tissue section was measured and statistically analyzed.

[0084] 3.2 Test Results: The results are shown in Table 7.

[0085] Table 7 Results of Zebrafish Skin Barrier Thickening Rate

[0086] As shown in Table 7, salicin at a test concentration of 0.015 g / L can significantly increase the thickness of the skin surface layer of zebrafish, thus enhancing the skin barrier.

[0087] Experimental Example 4: Thickening of the Dermis

[0088] 4.1 Experimental Methods: Three 12-month-old male zebrafish were randomly selected and exposed to a 0.1 g / L salicin solution, with a blank control group included. The solution was changed every other day. On the 7th day of exposure, the fish were anesthetized with tricaine, and a small piece of skin tissue was collected from the same body part, fixed, paraffin-embedded, stained with Sirius red, and then scanned using a panoramic scanner. The collagen thickness at five points in each tissue section was measured and statistically analyzed.

[0089] 4.2 Test Results: The results are shown in Table 8.

[0090] Table 8 Results of collagen thickening rate in zebrafish skin

[0091] As shown in Table 8, salicin samples at a test concentration of 0.1 g / L can significantly increase the collagen thickness of zebrafish skin, thus exhibiting anti-aging effects.

[0092] Examples 1-4 & Comparative Examples 1-10

[0093] Mix the raw materials according to the formula shown in Table 9 to obtain the composition.

[0094] Table 9. Composition formulations for the Examples and Comparative Examples (unit: g)

[0095] Experimental Example 5: Zebrafish Embryo Skin Barrier Permeability Test

[0096] 5.1 Test Method: Twenty-four 72-hour zebrafish embryos were exposed to a 5 g / L sample solution of the composition prepared in Example 2. A blank control group and a model control group were also set up. After 1 hour of exposure, the fish embryos were treated with sodium dodecyl sulfate solution (SDS) + sample solution for 40 minutes. Then, they were stained with sodium fluorescein for 30 minutes. Fluorescence imaging was used to measure the fluorescence intensity of the tail fin and statistical analysis was performed.

[0097] 5.2 Test Results

[0098] The results are shown in Table 10.

[0099] Table 10 Results of skin barrier permeability inhibition rate in zebrafish embryos

[0100] As shown in Table 10, the composition provided in Example 2 has a 72% inhibition rate of skin barrier permeability in zebrafish embryos, and has the effect of promoting skin barrier repair.

[0101] Experimental Example 6: Hyaluronidase Inhibition Test

[0102] 6.1 Test Method: The test was conducted according to T / JSRH 003-2023 "Test for Anti-allergic Activity of Cosmetic Raw Materials - Hyaluronidase Inhibition Method". See Table 11 for sample addition requirements.

[0103] Table 11 Hyaluronidase Experimental Procedure (Unit: μL)

[0104] Inhibition rate calculation formula

[0105] In the formula: a is the OD value of tube A; b is the OD value of tube B; c is the OD value of tube C; d is the OD value of tube D; each group is measured in parallel three times, and the data are presented as the average value; the inhibition rate results are retained to two decimal places.

[0106] 6.2 Test Results

[0107] The results are shown in Table 12.

[0108] Table 12 Results of Hyaluronidase Inhibition Test

[0109] Note: 1. The model control group sample was pure water. 2. The sample consisted of 1.50% salicin, 0.25% olive fruit extract, 0.25% glucosylrutin, and the remainder water, diluted 100 times with pure water before testing.

[0110] As shown in Table 12, the compositions provided in Examples 2 and 3 have a significant inhibitory effect on hyaluronidase.

[0111] Experimental Example 7 Antioxidant Test

[0112] 7.1 Test Method: The test was conducted according to "TSHRH006-2018 Cosmetics - Test Method for Free Radical (DPPH) Scavenging". See Table 13 for sample addition requirements.

[0113] Table 13 Sample Addition Requirements

[0114] Shake gently and let stand at room temperature for 5 minutes. Transfer each reaction solution into a 1 cm cuvette and measure the absorbance at 517 nm.

[0115] The free radical scavenging rate of the sample at each test concentration was calculated using the following formula:

[0116] In the formula: T—Absorbance of the sample tube, i.e., absorbance of the solution after the sample reacts with DPPH; T0—Sample background absorbance; The average of three absorbance values ​​of the C-DPPH tube, i.e. the absorbance of the DPPH solution without the addition of sample; C0 — Solvent background absorbance.

[0117] At the same time, the standard deviation (SD) of the clearance rate between parallel tubes in each group is calculated. The parallelism of the experiment is considered to be valid if the SD value is ≤3%.

[0118] 7.2 Test Results

[0119] The results are shown in Table 14.

[0120] Table 14 Free radical scavenging rate of samples at various concentrations

[0121] Note: 1. The sample concentrations in Table 14 are the initial concentrations, and the solvent is water. All samples were diluted 100 times with pure water before testing.

[0122] Q value calculation formula:

[0123] E AB E represents the DPPH free radical inhibition rate of the two drugs combined. A E represents the DPPH radical inhibition rate of single agent A. B The DPPH radical inhibition rate of single agent B.

[0124] If Q < 0.85, it means E A Ingredients and E B The components have antagonistic effects; if Q > 1.15, it indicates that E A Ingredients and E B The components have a synergistic effect; if 0.85 ≤ Q ≤ 1.15, it means E A Ingredients and E B The components have a normal additive effect.

[0125] As shown in Table 14, salicin, olive fruit extract and glucosylrutin have a synergistic antioxidant effect when the mass ratio is 0.8~3:0.2~0.35:0.1~0.3.

[0126] The above description is merely a preferred embodiment of this application, but the scope of protection of this application is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in this application, based on the technical solution and application concept of this application, should be included within the scope of protection of this application.

Claims

1. A composition, characterized in that, Including salicin, olive fruit extract, and glucosyl rutin-containing raw materials; The mass ratio of salicin, olive fruit extract and glucosylrutin raw material is 0.5~5:0.05~0.5:0.01~0.

5.

2. The composition according to claim 1, characterized in that, The mass ratio of salicin, olive fruit extract and glucosylrutin raw material is 0.8~3:0.2~0.35:0.1~0.

3.

3. The composition according to claim 1 or 2, characterized in that, The glucosylrutin-containing raw material comprises 50wt%~100wt% glucosylrutin, 0wt%~30wt% isoquercitrin, and the balance being water.

4. The composition according to claim 1 or 2, characterized in that, The olive fruit extract is an ethyl acetate extract of olive fruit.

5. The composition according to claim 4, characterized in that, The olive fruit extract contains more than 20 wt% hydroxytyrosol.

6. Use of the composition according to any one of claims 1 to 5 in the preparation of barrier repair, antioxidant, anti-aging and / or whitening products.

7. The use according to claim 6, characterized in that, The antioxidant mentioned refers to the scavenging of free radicals.

8. Use of salicin in the preparation of barrier repair and / or anti-aging products.

9. The use according to claim 8, characterized in that, The salicin enhances the expression of at least one of lipoprotein, filaggrin, and glutamine transferase 1; The salicin increases the thickness of the skin surface layer and / or the thickness of skin collagen.

10. A personal care product, characterized in that, Includes the composition according to any one of claims 1 to 5.

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