Skin enhancing agent compositions containing extracellular matrix and capable of inducing organoid regenerative factor expression

Abstract

The present application relates to a skin enhancer composition containing extracellular matrix, more specifically, the composition is composed of skin conditioner, osmotic pressure regulator, acidity regulator and purified water, and the skin conditioner is composed of hyaluronic acid, telopeptide collagen, glycosaminoglycan and water-soluble proteoglycan. The skin enhancer composition containing extracellular matrix composed of the above ingredients not only has excellent antioxidant, skin elasticity improving and skin barrier improving effects, but also has the effect of inducing organoid regeneration factor expression.

Description

Technical Field

[0001] This invention relates to a skin enhancer composition containing an extracellular matrix, and more specifically, to a skin enhancer composition containing an extracellular matrix that not only has excellent antioxidant, skin elasticity-enhancing, and skin barrier function-improving effects, but also induces the expression of organoid regeneration factors. Background Technology

[0002] As skin ages, its physiological activity gradually declines, leading to symptoms such as wrinkles, reduced elasticity, and dryness. With the increase in average human lifespan, the demand for cosmetics designed to improve these symptoms is also growing.

[0003] With the development of biochemistry and molecular biology, scientists have discovered trace amounts of signaling substances in the body, such as growth factors, and have re-established the theory of biological aging based on this. As we age, these signaling substances gradually decrease in the body. Research shows that this phenomenon is closely related to the reduction of collagen, elastin, and hyaluronic acid. With the decrease of these three components, signs of skin aging, such as decreased skin elasticity, increased wrinkles, and pigmentation, gradually appear.

[0004] Among all the organs in the human body, the skin is the largest, with an area of ​​approximately 1.8 square meters, a thickness of 2 to 4 millimeters, and a weight of about 3 kilograms. The skin is composed of several different layers, each with a specific function, primarily including the epidermis and dermis. The epidermis, located on the outermost layer of the skin, can be further divided into five parts: the stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum, and stratum basale.

[0005] Between the stratum lucidum and stratum granulosum in the epidermis lies a moisture barrier. This barrier possesses chemical properties that prevent the invasion of foreign substances and the loss of moisture from within the skin. Due to the presence of this moisture barrier, not only foreign substances but also water molecules have difficulty penetrating, thus hindering the deep penetration of active ingredients in cosmetics into the skin. The dermis comprises over 90% of the skin tissue and is known as the "true skin," determining its shape. The dermis is divided into the papillary layer and the reticular layer. The reticular layer, located beneath the papillary layer, is primarily composed of a strong and irregularly arranged reticular connective tissue, making up the majority of the dermis and mainly composed of collagen, elastin, and hyaluronic acid.

[0006] In recent years, the skin care field has gradually shifted from fillers or botulinum toxin injections to the use of skin boosters; however, no skin boosters containing extracellular matrix have been reported.

[0007] Existing technical documents

[0008] Patent documents

[0009] Patent document 0001, Korean Patent Publication No. 10-2018-0123312 (November 16, 2018).

[0010] Patent document 0002 Korean Patent Publication No. 10-2024-0139719 (September 24, 2024) Summary of the Invention

[0011] The purpose of this invention is to provide a skin enhancer composition containing an extracellular matrix, which not only has excellent antioxidant effects, improves skin elasticity and enhances skin barrier function, but also induces the expression of organoid regeneration factors.

[0012] The objective of this invention can be achieved by providing a skin-enhancing composition containing an extracellular matrix, the composition comprising a skin conditioning agent, an osmotic pressure regulator, an acidity regulator, and purified water, wherein the skin conditioning agent comprises hyaluronic acid, non-antigenic collagen, glycosaminoglycans, and water-soluble proteoglycans.

[0013] Preferably, the skin enhancer composition containing extracellular matrix provided by the present invention comprises 1% to 1.2% by weight of a skin conditioning agent, 0.7% to 0.9% by weight of an osmotic pressure regulator, 0.15% to 0.2% by weight of an acidity regulator, and the balance being purified water.

[0014] More preferably, according to the present invention, the skin conditioning agent is composed of the following components: based on 100 parts by weight of hyaluronic acid, 8.5 to 9 parts by weight of determinated collagen, 0.5 to 1 part by weight of glycosaminoglycan, and 0.5 to 1 part by weight of water-soluble proteoglycan.

[0015] More preferably, according to the present invention, the skin enhancer composition containing extracellular matrix further comprises: 0.1% to 1% by weight of an antioxidant component and 0.001% to 0.01% by weight of a polypeptide mixture, wherein the antioxidant component is selected from one or more of the group consisting of bakuchiol, idebenone, retinol, arbutin, nicotinamide and polyphenols.

[0016] More preferably, according to the present invention, the polypeptide mixture is composed of two or more components selected from the group consisting of acetyl hexapeptide-8, phenylalanine, leucine, methionine, lysine, isoleucine, valine, histidine, threonine and tryptophan.

[0017] The skin enhancer composition containing extracellular matrix provided by this invention not only has excellent antioxidant effects, improves skin elasticity and enhances the skin barrier, but also has a superior effect of significantly promoting the expression of organoid regeneration factors. Detailed Implementation

[0018] The preferred embodiments of the present invention and the physical properties of each component will be described in detail below, so as to enable those skilled in the art to easily implement the present invention, and not to limit the technical concept and protection scope of the present invention.

[0019] According to the present invention, the skin enhancer composition containing extracellular matrix is ​​composed of a skin conditioning agent, an osmotic pressure regulator, an acidity regulator and purified water. Preferably, the composition is composed of 1% to 1.2% by weight of the skin conditioning agent, 0.7% to 0.9% by weight of the osmotic pressure regulator, 0.15% to 0.2% by weight of the acidity regulator, and the balance being purified water.

[0020] The skin conditioning agent is present in an amount of 1% to 1.2% by weight, preferably composed of hyaluronic acid, determinated collagen, glycosaminoglycan, and water-soluble proteoglycan; more preferably, the skin conditioning agent is composed of the following components: based on 100 parts by weight of hyaluronic acid, 8.5 to 9 parts by weight of determinated collagen, 0.5 to 1 part by weight of glycosaminoglycan, and 0.5 to 1 part by weight of water-soluble proteoglycan. This skin conditioning agent in the composition has the effects of improving skin wrinkles, moisturizing, whitening, and anti-oxidation.

[0021] Hyaluronic acid is the main component of the skin conditioning agent in the skin-enhancing agent composition of this invention. It is a substance widely found in human and animal tissues. As a natural moisturizing factor, it plays a role in maintaining the stability of water content, tissue structure, and morphology, and lubricating the skin barrier in vivo. In addition, hyaluronic acid has extremely strong hydrophilicity, exhibits excellent water retention capacity, and can bind water to skin cells.

[0022] Furthermore, the content of the determinated peptide collagen is 8.5 to 9 parts by weight, and it is collagen with the antigenic terminal peptide removed. As an extracellular matrix component, this determinated peptide collagen not only exhibits excellent effects in skin regeneration and skin adaptability, but also can transmit various signals through interactions with cells. During the process of cell-extracellular matrix binding, it helps regulate cell behavior and promotes cell survival and growth.

[0023] Generally, collagen has a molecular weight of approximately 300,000 Daltons, consisting of three polypeptide chains with a molecular weight of approximately 100,000 Daltons intertwined to form a helical structure. At both ends of the collagen molecule are telopeptides that do not form a helical structure, and most of the antigenicity of collagen is located in these telopeptides. Therefore, collagen used in dermal applications preferably removes these antigenic telopeptides to reduce the risk of immune responses.

[0024] When the content of the determinated collagen is less than 8.5 parts by weight, the above-mentioned effects are not obvious; and when the content exceeds 9 parts by weight, the above-mentioned effects are not significantly improved, and relatively speaking, the content of hyaluronic acid, glycosaminoglycans and water-soluble proteoglycans is excessively reduced, which is not ideal.

[0025] In addition, the content of the glycosaminoglycan is 0.5 parts by weight to 1 part by weight. It is an ingredient with excellent effects in improving skin hydration and elasticity, and can provide excellent anti-aging effects. By delivering and retaining moisture to the skin, it helps to reduce wrinkles and improve skin texture.

[0026] When the content of the glycosaminoglycan is less than 0.5 parts by weight, the above-mentioned effects are not obvious; while when the content exceeds 1 part by weight, the above-mentioned effects are not significantly enhanced, and may cause skin irritation, so it is not ideal.

[0027] In addition, the content of the water-soluble proteoglycan is 0.5 parts by weight to 1 part by weight. As a representative extracellular matrix component, it can regulate the interaction between cells and the extracellular matrix, enhance cell migration ability, and give skin elasticity improvement, whitening and moisturizing effects.

[0028] When the content of the water-soluble proteoglycan is less than 0.5 parts by weight, the above-mentioned effects are not obvious; while when the content exceeds 1 part by weight, side effects such as skin tightness may occur, so it is not ideal.

[0029] When the content of the skin conditioning agent is less than 1% by weight, the above-mentioned effects are not obvious; while when the content exceeds 1.2% by weight, the above-mentioned effects are not significantly improved, and may cause side effects such as skin irritation or skin tightness, so it is not ideal.

[0030] The osmotic pressure regulator has a content of 0.7% to 0.9% by weight, preferably composed of sodium chloride. It promotes the excretion of waste products from the skin by inducing osmotic pressure, thereby improving skin inflammation and enhancing the whitening effect.

[0031] When the content of the osmotic pressure regulator is less than 0.7% by weight, the above-mentioned effects are not obvious; while when its content exceeds 0.9% by weight, the above-mentioned effects are not significantly improved, and the content of other components is relatively excessively reduced, which may reduce the functionality of the skin enhancer composition, and is therefore not ideal.

[0032] The acidity regulator is present in an amount of 0.15% to 0.2% by weight and is composed of sodium phosphate and disodium hydrogen phosphate, more preferably, a mixture of sodium phosphate and disodium hydrogen phosphate in a weight ratio of 1:5. The function of this acidity regulator is to adjust the pH value of the skin enhancer, thereby achieving low irritation and imparting excellent preservation properties to the composition.

[0033] When the content of the acidity regulator is less than 0.15% by weight, the above-mentioned effects are not obvious; while when the content exceeds 0.2% by weight, the above-mentioned effects are not significantly enhanced, and the content of other components is relatively excessively reduced, which may reduce the functionality of the skin enhancer composition, and is therefore not ideal.

[0034] Furthermore, the skin-enhancing composition containing extracellular matrix of the present invention may further include antioxidant components and a polypeptide mixture, preferably, the content of said antioxidant component is 0.1% to 1% by weight, and the content of said polypeptide mixture is 0.001% to 0.01% by weight.

[0035] As described above, the addition of antioxidants and peptide mixtures not only enhances the antioxidant effect of the skin enhancer composition prepared by the present invention, but also significantly improves its efficacy in improving skin wrinkles and promoting skin regeneration, thereby providing a skin enhancer composition with superior efficacy.

[0036] The antioxidant component is present in an amount of 0.1% to 1% by weight, preferably selected from one or more of the group consisting of bakuchiol, idebenone, retinol, arbutin, niacinamide, and polyphenols. More preferably, the antioxidant component consists of 100 parts by weight of bakuchiol and 0.5 to 1.5 parts by weight of idebenone. The antioxidant component composed of bakuchiol and idebenone not only further enhances the antioxidant effect but also has a good skin protective effect.

[0037] The psoralen is a natural component derived from psoralen seeds. It belongs to the subterpenoid class of terpenoid alcohols and has excellent antioxidant activity, which can play a role in anti-skin aging and improving wrinkles.

[0038] Furthermore, idebenone is a coenzyme Q (Ubiquinone)-like antioxidant found in active cell membranes, with a structure similar to coenzyme Q10. Coenzyme Q10 is mainly involved in the electron transfer chain (ETC) in mitochondria, which is related to the synthesis of cellular energy ATP (adenosine triphosphate). Its chemical name is 2,3-dimethoxy-5-methyl-6-(10-hydroxydecyl)-1,4-benzoquinone.

[0039] In addition, idebenone plays a significant role in promoting cell activity by participating in the electron transport chain (ETC) related to the generation of cellular energy ATP (adenosine triphosphate) in mitochondria, and can inhibit the generation of reactive oxygen species (ROS) in mitochondria, thereby protecting the skin.

[0040] When the content of the antioxidant component is less than 0.1 parts by weight, the above-mentioned effects are not obvious; and when the content exceeds 1 part by weight, the above-mentioned effects are not significantly enhanced, and may lead to a significant increase in the production cost of the skin enhancer composition prepared by the present invention and reduce the stability of its formulation, which is not ideal.

[0041] Furthermore, the content of the polypeptide mixture is 0.001 parts by weight to 0.01 parts by weight, and it is preferably composed of two or more components selected from the group consisting of acetyl hexapeptide-8, phenylalanine, leucine, methionine, lysine, isoleucine, valine, histidine, threonine and tryptophan.

[0042] Acetyl hexapeptide-8, also known as "botulinum toxin peptide" due to its botulinum toxin-like effects, inhibits wrinkle formation by reducing the release of acetylcholine to suppress muscle contraction and promotes collagen production, thus giving the skin a regenerative effect.

[0043] In addition, the phenylalanine, leucine, methionine, lysine, isoleucine, valine, histidine, threonine and tryptophan can impart skin whitening and regeneration effects, thereby playing a role in achieving skin whitening and enhancing the skin barrier.

[0044] When the content of the polypeptide mixture is less than 0.001 parts by weight, the above-mentioned effects are not obvious; while when the content exceeds 0.01 parts by weight, the above-mentioned effects are not significantly enhanced, and the production cost of the skin enhancer composition is significantly increased, which is not ideal.

[0045] The following examples illustrate the preparation method of the skin enhancer composition containing extracellular matrix provided by the present invention, as well as the physical properties of the composition prepared by the method.

[0046] Preparation Example 1. Preparation of Skin Conditioning Agent

[0047] A skin conditioning agent was prepared by mixing 100 parts by weight of hyaluronic acid, 8.7 parts by weight of determinated collagen, 0.63 parts by weight of glycosaminoglycan, and 0.67 parts by weight of water-soluble proteoglycan.

[0048] Preparation Example 2. Preparation of Antioxidant Components

[0049] An antioxidant component was prepared by mixing 100 parts by weight of psoralen with 1.1 parts by weight of idebenone.

[0050] Preparation Example 3. Preparation of Polypeptide Mixtures

[0051] Acetyl hexapeptide-8, phenylalanine, leucine, methionine, lysine, isoleucine, valine, histidine, threonine, and tryptophan were mixed in a weight ratio of 1:1:1:1:1:1:1:1:1:1 to prepare a polypeptide mixture.

[0052] Example 1

[0053] 1.1% by weight of the skin conditioning agent prepared in Preparation Example 1, 0.8% by weight of the osmotic pressure regulator (sodium chloride), 0.18% by weight of the acidity regulator (sodium phosphate and disodium hydrogen phosphate mixed in a weight ratio of 1:5), and the balance of purified water were added to a mixing device equipped with a stirrer and stirred at 150 rpm for 30 minutes to obtain a skin enhancer composition containing extracellular matrix.

[0054] Example 2

[0055] The same method as in Example 1 was followed, but 1.0 wt% of the skin conditioning agent, 0.7 wt% of the osmotic pressure regulator, 0.15 wt% of the acidity regulator, and the balance of purified water were stirred to prepare a skin enhancer composition containing extracellular matrix.

[0056] Example 3

[0057] The same method as in Example 1 was followed, but 1.2% by weight of the skin conditioning agent, 0.9% by weight of the osmotic pressure regulator, 0.2% by weight of the acidity regulator, and the balance of purified water were stirred to obtain a skin enhancer composition containing extracellular matrix.

[0058] Example 4

[0059] The same method as in Example 1 was followed, but 0.5% by weight of the antioxidant component prepared in Preparation Example 2 and 0.005% by weight of the polypeptide mixture prepared in Preparation Example 3 were further added and mixed to obtain a skin enhancer composition containing extracellular matrix.

[0060] Example 5

[0061] The same method as in Example 2 was followed, but 0.5% by weight of the antioxidant component prepared in Preparation Example 2 and 0.005% by weight of the polypeptide mixture prepared in Preparation Example 3 were further added and mixed to obtain a skin enhancer composition containing extracellular matrix.

[0062] Example 6

[0063] The same method as in Example 3 was followed, but 0.5% by weight of the antioxidant component prepared in Preparation Example 2 and 0.005% by weight of the polypeptide mixture prepared in Preparation Example 3 were further added and mixed to obtain a skin enhancer composition containing extracellular matrix.

[0064] Comparative Example 1

[0065] Two parts by weight of polynucleotide were added to 100 parts by weight of purified water and stirred at 22°C for 120 minutes to obtain a polynucleotide aqueous solution. Then, 100 parts by weight of the obtained polynucleotide aqueous solution were mixed with 0.01 parts by weight of nicotinamide mononucleotide and 0.01 parts by weight of nicotinamide adenine dinucleotide and stirred at 150 rpm for 60 minutes to homogenize it, thus obtaining a skin enhancer composition.

[0066] Comparative Example 2

[0067] The same method as in Example 1 was followed, but only 0.5% by weight of the skin conditioning agent prepared in Preparation Example 1 was used for mixing to obtain a skin enhancer composition containing extracellular matrix.

[0068] Comparative Example 3

[0069] The same method as in Example 1 was followed, but 2% by weight of the skin conditioning agent prepared in Preparation Example 1 was used for mixing to obtain a skin enhancer composition containing extracellular matrix.

[0070] The skin-enhancing agent compositions prepared by Examples 1-6 and Comparative Examples 1-3 were tested for their effects on improving skin wrinkles, enhancing skin elasticity, improving skin moisturizing ability, whitening effect, and skin irritation. The results are shown in Table 1.

[0071] The determination of the skin enhancer composition in terms of improving skin wrinkles, enhancing skin elasticity, enhancing skin moisturizing ability and whitening effect was carried out by 50 adult male and female subjects aged 30 to 50 years. The subjects applied 1 mL of the prepared skin enhancer to the neck twice a day at 12-hour intervals for 15 consecutive days. The results were evaluated using a 5-point scale and the average value was used to represent the evaluation results.

[0072] 5 points: Significant improvement; 4 points: Mild improvement; 3 points: No significant change; 2 points: Some deterioration; 1 point: Significant deterioration.

[0073] In addition, skin irritation is graded according to the following criteria.

[0074] ◎: High stimulation, ○: Stimulation present, △: Mild stimulation, ×: No stimulation

[0075]

[0076] distinguish Skin wrinkles Skin elasticity Skin moisturizing Skin whitening Skin irritation Example 1 4.5 4.6 4.6 4.5 x Example 2 4.4 4.5 4.5 4.4 x Example 3 4.6 4.6 4.6 4.5 x Example 4 4.7 4.7 4.6 4.5 x Example 5 4.5 4.6 4.5 4.4 x Example 6 4.7 4.8 4.6 4.6 x Comparative Example 1 4.1 4.2 4.3 4.2 △ Comparative Example 2 3.5 3.6 3.7 3.6 x Comparative Example 3 4.5 4.5 4.5 4.4 △

[0077] As shown in Table 1, compared with the skin enhancer compositions prepared in Comparative Examples 1-2, the skin enhancer compositions prepared in Examples 1-6 of the present invention are superior in improving skin wrinkles, enhancing skin elasticity, enhancing skin moisturizing power and whitening effect; and compared with the skin enhancers prepared in Comparative Examples 1 and 3, they have lower skin irritation.

[0078] In addition, the skin barrier improvement effect of the skin enhancer compositions prepared by Example 1, Example 4 and Comparative Example 1 was measured, and the results are shown in Tables 2 and 3 below.

[0079] The skin barrier improvement effect was evaluated by measuring its repair effect on damaged skin barriers. The method for measuring the repair effect was as follows: After cleaning the forearms of 20 adult male and female subjects aged 30 to 50 years, the experiment was conducted after the forearms were left to stand for 30 minutes under constant temperature and humidity conditions (20 to 24°C, relative humidity 40 to 50%).

[0080] To evaluate the effects of various skin enhancer compositions on the repair efficacy of skin barrier damage caused by external physical stimuli, the following experiment was conducted: The skin barrier of the subjects' forearms was damaged using a tape stripping method, followed by application of various prepared skin enhancer compositions. After one week of continuous use, transepidermal water loss (TEWL, g / m²) was measured. 2The repair and soothing effects were evaluated using the transepidermal water loss (Vapometer, Delfin, Finland) and skin erythema average (Redness average). A control group without any product application was used in the experiment. Transepidermal water loss and skin erythema average were measured using a Vapometer (transepidermal water loss measuring instrument, Delfin, Finland) and an Antera 3D imaging system (Miravex, Ireland), respectively.

[0081]

[0082]

[0083] Table 3

[0084]

[0085]

[0086] As shown in Table 2, the skin enhancer compositions prepared by Examples 1 and 4 of the present invention showed a decrease in transepidermal water loss (TEWL) values ​​after one use and after one week of continuous use in the determination of transepidermal water loss after physical stimulation-induced skin barrier damage, indicating that they can effectively improve skin barrier function.

[0087] In contrast, the skin-enhancing composition prepared by Comparative Example 1 showed a higher transdermal water loss, indicating a weaker effect in improving the skin barrier.

[0088] Furthermore, as shown in Table 3, the skin enhancer compositions prepared by Examples 1 and 4 of the present invention showed a decrease in erythema value after physical stimulation compared to the initial skin erythema value, both after one application and after one week of continuous use, indicating that they have a good sedative effect. In particular, the skin enhancer composition prepared by Example 4 has a more superior effect on improving the skin barrier.

[0089] In contrast, the skin enhancer composition prepared by Comparative Example 1 had a relatively high skin erythema value, indicating that it was less effective in improving the skin barrier.

[0090] Therefore, the skin enhancer composition containing extracellular matrix provided by the present invention not only has excellent antioxidant, skin elasticity enhancement and skin barrier improvement effects, but also induces the expression of organoid regeneration factors.

Claims

1. A skin-enhancing composition containing an extracellular matrix, characterized in that, The composition comprises a skin conditioning agent, an osmotic pressure regulator, an acidity regulator, and purified water. The skin conditioning agent is composed of hyaluronic acid, determinated collagen, glycosaminoglycans, and water-soluble proteoglycans.

2. The skin-enhancing composition containing extracellular matrix according to claim 1, characterized in that, The skin enhancer composition containing extracellular matrix comprises the following components: 1% to 1.2% by weight of skin conditioning agent, 0.7% to 0.9% by weight of osmotic pressure regulator, 0.15% to 0.2% by weight of acidity regulator, and the balance being purified water.

3. The skin-enhancing composition containing extracellular matrix according to claim 1 or 2, characterized in that, The skin conditioning agent is composed of the following components mixed in the following weight ratios: Based on 100 parts by weight of hyaluronic acid, 8.5 to 9 parts by weight of determinated collagen, 0.5 to 1 part by weight of glycosaminoglycan, and 0.5 to 1 part by weight of water-soluble proteoglycan.

4. The skin-enhancing composition containing extracellular matrix according to claim 1, characterized in that, The skin enhancer composition containing extracellular matrix further comprises 0.1% to 1% by weight of antioxidant components and 0.001% to 0.01% by weight of a polypeptide mixture; The antioxidant component is selected from one or more of the following groups: bakuchiol, idebenone, retinol, arbutin, nicotinamide, and polyphenols.

5. The skin-enhancing composition containing extracellular matrix according to claim 4, characterized in that, The polypeptide mixture consists of two or more components selected from the group consisting of acetyl hexapeptide-8, phenylalanine, leucine, methionine, lysine, isoleucine, valine, histidine, threonine, and tryptophan.

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