Application of Angelica dahurica extract in improving energy metabolism and liver injury of perinatal dairy cows

Abstract

The application belongs to the technical field of biological medicine, and provides application of white peony root extract in improvement of perinatal cow energy metabolism and liver damage, wherein the white peony root extract is extracted by an ethanol infiltration method, and main components include ligustilide, imperatorin and isoimperatorin. The experiment proves that the white peony root extract can effectively improve energy metabolism disorder and metabolic liver damage of ketosis cows and increase milk yield, and can be used as a medicine for treating perinatal cow metabolic disorder diseases, and can be used as a feed additive, thereby laying a solid foundation for quality improvement and efficiency increase of the dairy industry.

Description

Technical Field

[0001] This invention belongs to the field of biomedical technology, and in particular relates to the application of Angelica dahurica extract in improving energy metabolism and liver damage in periparturient dairy cows. Background Technology

[0002] The peripartum period (3 weeks before calving to 3 weeks after calving) is a critical stage in the dairy cow's production cycle. During this period, the cow's physiological and metabolic state undergoes drastic changes, making it highly susceptible to metabolic diseases. During the peripartum period, the cow's dry matter intake (DMI) drops sharply, and feed intake cannot meet the body's nutritional needs. Meanwhile, the initiation of lactation causes a surge in the cow's energy demand, resulting in a "supply-demand imbalance." This leads to metabolic disorders and ultimately causes the cow to enter a state of negative energy balance (NEB). When a cow is in a state of NEB (non-lactated fat), it mobilizes its stored adipose tissue to promote fat synthesis and breakdown to make up for the energy gap required by the body. At the same time, the initiation of lactation causes the mammary glands to preferentially use glucose to produce lactose. Therefore, during this process, triglycerides (TG) stored in adipose tissue are broken down by lipases into glycerol and non-esterified fatty acids (NEFA), which are released into the bloodstream. Due to the increase in hepatic blood flow and plasma NEFA concentration, the liver takes up excessive NEFA in a concentration-dependent manner, resulting in abnormal accumulation of lipids in the liver. NEFA enters the mitochondria and undergoes β-oxidation to generate acetyl-CoA, which enters the tricarboxylic acid cycle (TCA) to provide energy for the body. However, postnatal TCA cycle intermediates (such as oxaloacetate) are depleted due to excessive gluconeogenesis, leading to impaired TCA cycle and oxidative phosphorylation. As a result, large amounts of NEFA cannot be completely oxidized, generating ketone bodies and inducing clinical or subclinical ketosis. Furthermore, accumulated NEFA produces large amounts of reactive oxygen species (ROS), which attack polyunsaturated fatty acids on cell membranes and organelle membranes, triggering lipid peroxidation. This process damages the integrity of the mitochondrial membrane, leading to the loss of mitochondrial DNA. The release of NEFA (mtDNA) activates pathways such as inflammation and endoplasmic reticulum stress, leading to metabolic liver damage. Subsequently, the liver's redox capacity decreases, and excess NEFA is esterified in the body to synthesize TG, which is deposited in large quantities in the liver, forming fatty liver. In addition, metabolic liver damage can also lead to decreased blood glucose and a persistent negative energy balance in dairy cows during the peripartum period, resulting in a decline in milk production performance throughout the lactation period, which seriously restricts the realization of dairy cow production performance and the improvement of breeding efficiency.

[0003] Angelica dahurica, specifically the dried root of Angelica dahurica and its variant, *Angelica dahurica*, belonging to the Apiaceae family, is used medicinally. It is oblong-conical in shape, with a grayish-brown surface, longitudinal wrinkles, and transverse lenticel-like protrusions. The root head is bluntly quadrangular. It is firm in texture, with a white, powdery cross-section, a nearly square brown cambium ring, and numerous brown oil dots in the cortex. It has an aromatic odor and a pungent, slightly bitter taste. Its core functions are to relieve exterior syndromes and dispel cold, dispel wind and relieve pain, clear nasal passages, dry dampness and stop leukorrhea, and reduce swelling and drain pus. The main components of Angelica dahurica are coumarins, followed by volatile oils and polysaccharides. The coumarins are mainly found in the root of Angelica dahurica, including angelicin, imperatorin, isoimperatorin, and oxyimperatorin, which have significant antioxidant and lipid metabolism regulating effects. However, it remains unclear whether it can be used in peripartum dairy cows to alleviate negative energy balance and metabolic liver damage. Summary of the Invention

[0004] The purpose of this invention is to provide the application of Angelica dahurica extract in improving energy metabolism and liver damage in periparturient dairy cows, thereby addressing the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: The application of Angelica dahurica extract in the preparation of products that improve energy metabolism and liver damage in periparturient dairy cows and increase milk production. The Angelica dahurica extract is extracted by ethanol percolation method, and its main components include angelicin, imperatorin, and isoimperatorin.

[0006] Compared with the prior art, the specific beneficial effects of the present invention are as follows: This invention confirms that Angelica dahurica extract can effectively improve energy metabolism disorders and metabolic liver damage in periparturient dairy cows and increase milk production. It can be used as a drug to treat periparturient ketosis in dairy cows and as a feed additive, laying a solid foundation for improving the quality and efficiency of the dairy industry. Attached Figure Description

[0007] Figure 1 The results of the effect of Angelica dahurica extract provided in Example 1 of the present invention on the energy metabolism indicators of peripartum dairy cows are shown. A is the content of non-esterified fatty acids (NEFA) in the blood, B is the content of β-hydroxybutyrate (BHBA) in the blood, and C is the content of glucose (GLU) in the blood. Figure 2 The results of the effect of Angelica dahurica extract provided in Example 1 of the present invention on liver injury indicators in periparturient dairy cows are shown. A is the content of alanine aminotransferase (ALT) in the blood, B is the content of aspartate aminotransferase (AST) in the blood, and C is the content of glutamate dehydrogenase (GLDH) in the blood. Figure 3 The results of the effect of Angelica dahurica extract provided in Example 1 of the present invention on milk yield in peripartum dairy cows; Figure 4 The results of the effect of Angelica dahurica extract provided in Example 2 of the present invention on energy metabolism indicators of dairy cows with ketosis are shown in Figure 2. A represents the content of NEFA in the blood, B represents the content of BHBA in the blood, and C represents the content of GLU in the blood. Figure 5 The results of the effect of Angelica dahurica extract provided in Example 2 of the present invention on liver damage indicators in dairy cows with ketosis are shown. A is the content of ALT in the blood, B is the content of AST in the blood, and C is the content of GLDH in the blood. Figure 6 The results of the effect of Angelica dahurica extract provided in Example 2 of the present invention on the milk yield of dairy cows with ketosis; In the attached diagram, This is expressed as a significant difference compared to the control group ( P <0.05); This indicates a highly significant difference compared to the control group. P <0.01). Detailed Implementation

[0008] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0009] The specific implementation of the present invention will be described in detail below with reference to specific embodiments.

[0010] Example 1: Improvement of metabolic liver injury in peripartum dairy cows by Angelica dahurica extract: Forty healthy peripartum dairy cows from a dairy farm in Changchun City, Jilin Province, were selected and divided into four groups of 10 cows each: a control group, a low-dose Angelica dahurica group (1 part / day / cow), a medium-dose Angelica dahurica group (2 parts / day / cow), and a high-dose Angelica dahurica group (3 parts / day / cow). Angelica dahurica extract was fed every other day for three weeks before and three weeks after calving (refer to Huang Jiajia, Long Xiaoyan, Wang Rui, et al. Optimization of percolation extraction process of coumarin components in Angelica dahurica [J]. Traditional Chinese Medicine, 2019, 41(9):2204-2206). After pulverizing Angelica dahurica, it was soaked in 8 times the amount of 60% ethanol for 12 hours at a percolation rate of 4 mL / (min). (kg), the percolate was collected and concentrated to obtain the extract. After the feeding experiment, blood was collected from the tail vein of dairy cows before morning feeding (05:00-06:00). Biochemical reagent kits were used to detect energy metabolism indicators such as NEFA, BHBA, and GLU in the blood; liver damage indicators such as ALT, AST, and GLDH; and changes in milk production of dairy cows were recorded.

[0011] Depend on Figure 1As shown in AB, Angelica dahurica extract can reduce the levels of NEFA and BHBA in the blood of dairy cows. Figure 1 As can be seen from the middle C, feeding with Angelica dahurica extract can significantly increase the GLU content in the blood of dairy cows, indicating that feeding with Angelica dahurica extract can improve the lipid metabolism status of dairy cows, and the effect is more significant at medium and high doses of Angelica dahurica extract. Depend on Figure 2 As observed in the AC study, Angelica dahurica extract significantly reduced the levels of blood ALT, AST, and GLDH, indicating that feeding Angelica dahurica extract can alleviate liver damage in dairy cows. Depend on Figure 3 It is evident that Angelica dahurica extract can increase milk production in dairy cows during the peripartum period.

[0012] Example 2: Improvement of metabolic liver damage in dairy cows with ketosis by Angelica dahurica extract: Twenty dairy cows with ketosis (blood BHBA > 1.2 mmol / L) within one week postpartum were selected from a dairy farm in Changchun City, Jilin Province. They were divided into two groups: a control group and a group receiving Angelica dahurica extract supplementation. Both groups were fed a medium dose of Angelica dahurica (2 doses / day / cow) every other day for three weeks. After the feeding period, tail vein blood was collected from the cows before morning feeding (05:00-06:00). Biochemical reagent kits were used to detect energy metabolism indicators such as NEFA, BHBA, and GLU; liver damage indicators such as ALT, AST, and GLDH; and changes in milk production were recorded. Depend on Figure 4 As observed in the AC study, medium-dose Angelica dahurica extract significantly reduced the levels of NEFA and BHBA in the blood of ketotic dairy cows and increased the level of GLU, indicating that medium-dose Angelica dahurica extract can effectively improve the disordered energy metabolism in ketotic dairy cows. Depend on Figure 5 The results showed that medium-dose Angelica dahurica extract significantly reduced the levels of ALT, AST, and GLDH in the blood of dairy cows with ketosis, indicating that medium-dose Angelica dahurica extract can effectively alleviate liver damage in dairy cows with ketosis. Depend on Figure 6 It is evident that medium-dose Angelica dahurica extract can significantly increase milk production in dairy cows with ketosis.

[0013] In conclusion, Angelica dahurica extract can effectively improve energy metabolism disorders and metabolic liver damage in peripartum dairy cows, and increase milk production.

[0014] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. Application of Angelica dahurica extract in the preparation of products that improve energy metabolism, liver damage, and milk yield in dairy cows with peripartum or ketosis.

2. The application according to claim 1, characterized in that, The Angelica dahurica extract was extracted by ethanol percolation and its main components include angelicin, imperatorin, and isoimperatorin.

3. The application according to claim 1, characterized in that, The Angelica dahurica extract reduces the levels of NEFA and BHBA in the blood of dairy cows, increases the level of GLU in the blood, and increases milk production, thereby improving the energy metabolism status of dairy cows during the peripartum period.

4. The application according to claim 1, characterized in that, The Angelica dahurica extract reduces the activity of blood liver damage markers AST, ALT, and GLDH.

5. The application according to claim 1, characterized in that, The drugs or feed additives prepared from the Angelica dahurica extract are used to improve energy metabolism and liver damage in periparturient dairy cows.

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