Application of Arabidopsis thaliana SPL7 gene in regulating seed number per silique

Abstract

The application discloses Arabidopsis thaliana SPL7 gene in regulating single-siliqua seed number. The application belongs to the technical field of biotechnology, and particularly relates to application of Arabidopsis thaliana SPL7 gene in regulating single-siliqua seed number. The SPL7 protein and the coding gene thereof in the application can be applied to the following aspects: 1) application in regulating single-siliqua seed number of Arabidopsis thaliana; 2) application in preparing a product for regulating single-siliqua seed number of Arabidopsis thaliana; 3) application in cultivating Arabidopsis thaliana with changed single-siliqua seed number; 4) application in preparing a product for cultivating Arabidopsis thaliana with changed single-siliqua seed number; and 5) application in breeding of Arabidopsis thaliana. SPL7 The application has breeding value in regulating single-siliqua seed number of Arabidopsis thaliana.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology, specifically relating to Arabidopsis thaliana. SPL7 Application of genes in regulating the number of seeds in siliques. Background Technology

[0002] With the continuous growth of the global population, the demand for food is increasing year by year; however, due to various factors such as human activities and climate change, arable land is constantly decreasing. Therefore, increasing food production is an urgent requirement for human life. The number of seeds in a silique is one of the three factors affecting yield and is an important breeding objective. The determining factors for the number of seeds in a silique are the number of ovules and the seed setting rate. The former determines the maximum number of seeds in a silique, while the latter is the proportion of ovules that convert into mature seeds.

[0003] The seed number of siliques is regulated by a multi-gene regulatory network. Although existing research has revealed a series of genes regulating the seed number of siliques, our understanding of the molecular mechanisms behind this trait remains very limited. SPL7 Belonging to the SPL gene family, whose members encode proteins containing an SPB-box, which is the core functional domain of the protein. SPL7 is a transcription factor that binds to the GTAC-motif in the promoter region of downstream genes. SPL7 It can promote the expression of four downstream miRNAs, namely miR397, miR398, miR408, and miR857, thereby leading to a reduction in their target transcripts, such as... CSD1 , CSD2 and LACs Therefore, for SPL7 Detailed studies of the functions can provide guidance for crop breeding work. Summary of the Invention

[0004] The main problem this invention aims to solve is how to regulate the number of seeds in Arabidopsis thaliana horns.

[0005] To address the aforementioned problems, the present invention provides the use of SPL7 protein, a substance regulating the expression of the gene encoding said protein, or a substance regulating the activity or content of said protein in any of the following: 1) Application in regulating the number of seeds in Arabidopsis thaliana siliques; 2) Application in the preparation of products that regulate the number of seeds in Arabidopsis thaliana siliques; 3) Application in cultivating Arabidopsis thaliana with altered seed number in siliques; 4) Application in the preparation of Arabidopsis products with altered seed number in siliques; 5) Application in Arabidopsis thaliana breeding; The protein is any of the following proteins: a1) A protein with the amino acid sequence SEQ ID No:1; a2) A protein having the same function as the amino acid sequence shown in SEQ ID No:1, but with one or more amino acid residues substituted and / or deleted and / or added. a3) Proteins that share more than 80% identity with the amino acid sequence defined by a1) or a2) and have the same function; a4) A fusion protein obtained by attaching a tag to the end of any of the proteins defined in a1)-a3).

[0006] To facilitate the purification or detection of the protein in a1), a tag protein can be attached to the amino or carboxyl terminus of the protein, which consists of the amino acid sequence shown in SEQ ID No:1 in the sequence listing.

[0007] The tag proteins include, but are not limited to: GST (glutathione thiotransferase) tag protein, His6 tag protein (His-tag), MBP (maltose-binding protein) tag protein, Flag tag protein, SUMO tag protein, HA tag protein, Myc tag protein, eGFP (enhanced green fluorescent protein), eCFP (enhanced cyan fluorescent protein), eYFP (enhanced yellow-green fluorescent protein), mCherry (monomer red fluorescent protein), or AviTag tag protein.

[0008] The proteins mentioned above can be synthesized artificially, or their encoding genes can be synthesized first and then expressed biologically.

[0009] Those skilled in the art can readily mutate the nucleotide sequence encoding the protein SPL7 of this invention using known methods, such as directed evolution or point mutation. Any artificially modified nucleotides that have 75% or more of the nucleotide sequence identity with the protein SPL7 isolated in this invention, as long as they encode and function as protein SPL7, are derived from and equivalent to the nucleotide sequence of this invention.

[0010] The aforementioned 75% or higher degree of identity can be 80%, 85%, 90%, or 95% or higher degree of identity.

[0011] In this article, identity refers to the similarity between amino acid sequences or nucleotide sequences. The identity of amino acid or nucleotide sequences can be determined using homology search sites on the Internet, such as the BLAST page on the NCBI homepage. For example, in Advanced BLAST 2.1, using blastp as the procedure, setting the Expect value to 10, setting all filters to OFF, using BLOSUM62 as the matrix, setting the Gap existence cost, Per residue gap cost, and Lambda ratio to 11, 1, and 0.85 (default values) respectively, and performing a search to calculate the identity of a pair of amino acid or nucleotide sequences, then the identity value (%) can be obtained.

[0012] In this document, the 80% or more of identity can be at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity.

[0013] In this document, the above 90% identity can be at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity.

[0014] In this article, the substance that regulates the activity and / or content of the protein may be a substance that regulates gene expression, wherein the gene encodes the protein SPL7.

[0015] In the above text, the substance that regulates gene expression can be a substance that performs at least one of the following six types of regulation: 1) Regulation occurring at the transcriptional level of the aforementioned gene; 2) Regulation that occurs after the gene is transcribed (i.e., regulation of the splicing or processing of the primary transcript of the gene). 3) Regulation of RNA transport of the gene (that is, regulation of the transport of mRNA of the gene from the nucleus to the cytoplasm). 4) Regulation of the translation of the aforementioned genes; 5) Regulation of mRNA degradation of the aforementioned gene; 6) Post-translational regulation of the gene (i.e., regulation of the activity of the protein translated from the gene).

[0016] In this invention, the regulation can be up-regulation, enhancement, or increase. The regulation can also be suppression, reduction, or down-regulation.

[0017] In this article, the substance that regulates gene expression or the substance that regulates the activity or content of the protein is a biological material related to the protein described in the preceding application, and the biological material may be any of the following: c1) The nucleic acid molecule encoding the protein; c2) An expression cassette containing the nucleic acid molecule described in c1); c3) A recombinant vector containing the nucleic acid molecule described in c1), or a recombinant vector containing the expression cassette described in c2); c4) Recombinant microorganisms containing the nucleic acid molecules described in c1), or recombinant microorganisms containing the expression cassette described in c2), or recombinant microorganisms containing the recombinant vector described in c3); c5) A transgenic plant cell line containing the nucleic acid molecule described in c1), or a transgenic plant cell line containing the expression cassette described in c2); c6) Transgenic plant tissue containing the nucleic acid molecules described in c1), or transgenic plant tissue containing the expression cassette described in c2); c7) A transgenic plant organ containing the nucleic acid molecule described in c1), or a transgenic plant organ containing the expression cassette described in c2); e1) Nucleic acid molecules that inhibit, reduce, or silence the expression of the protein-coding genes mentioned above; e2) An expression cassette containing the nucleic acid molecule described in e1); e3) A recombinant vector containing the nucleic acid molecule described in e1), or a recombinant vector containing the expression cassette described in e2); e4) Recombinant microorganisms containing the nucleic acid molecules described in e1), or recombinant microorganisms containing the expression cassette described in e2), or recombinant microorganisms containing the recombinant vector described in e3); e5) A transgenic plant cell line containing the nucleic acid molecule described in e1), or a transgenic plant cell line containing the expression cassette described in e2); e6) Transgenic plant tissue containing the nucleic acid molecules described in e1), or transgenic plant tissue containing the expression cassette described in e2); e7) A transgenic plant organ containing the nucleic acid molecule described in e1) or a transgenic plant organ containing the expression cassette described in e2).

[0018] In the above applications, c1) the nucleic acid molecule can be any of the following DNA molecules: d1) The nucleotide sequence is the DNA molecule shown in SEQ ID No:3; d2) The coding sequence is the DNA molecule shown in SEQ ID No:2; d3) has 90% or more identity with the nucleotide sequence defined by d1) or d2) and encodes a DNA molecule that encodes the protein described above; d4) A DNA molecule that hybridizes under strict conditions with a nucleotide sequence defined by d1) or d2) and encodes the protein described above.

[0019] The nucleic acid molecules mentioned in this article can be DNA, such as cDNA, genomic DNA, or recombinant DNA; the nucleic acid molecules can also be RNA, such as gRNA, mRNA, siRNA, shRNA, sgRNA, miRNA, or antisense RNA.

[0020] The SPL7 The encoding gene originated from natural strains of Arabidopsis thaliana from the Yangtze River population. SPL7 Dominant haplotypes of genes SPL7 Hap1 .

[0021] The vectors described herein are well-known to those skilled in the art and include, but are not limited to: plasmids, bacteriophages (such as λ phage or M13 filamentous phage), granules (i.e., Cosmids), Ti plasmids, or viral vectors. Specifically, it may be the vector pCAMBIA1300.

[0022] Recombinant expression vectors containing the SPL7 gene can be constructed using existing plant expression vectors. These plant expression vectors include, but are not limited to, binary Agrobacterium vectors and vectors suitable for plant microbombardment. The plant expression vectors may also contain the 3' untranslated region of the exogenous gene, i.e., containing a polyadenylate signal and any other DNA fragment involved in mRNA processing or gene expression. The polyadenylate signal can guide the addition of polyadenylate to the 3' end of the mRNA precursor; similar functions exist for the untranslated regions transcribed at the 3' end of genes including, but not limited to, Agrobacterium crown gall-inducing (Ti) plasmid genes (such as the Nos gene for lipase synthesis) and plant genes (such as the soybean storage protein gene).

[0023] The present invention also provides a method for altering the number of seeds in Arabidopsis thaliana siliques, the method comprising steps P or M, wherein step P is to enhance, increase, or upregulate the activity and / or content of the aforementioned protein in the target Arabidopsis thaliana, or / and enhance, increase, or upregulate the expression level of the gene encoding the aforementioned protein, thereby increasing the number of seeds in Arabidopsis thaliana siliques; and step M is to inhibit, decrease, or silence the activity and / or content of the aforementioned protein in the target Arabidopsis thaliana, or / and inhibit, decrease, or downregulate the expression level of the gene encoding the aforementioned protein, thereby decreasing the number of seeds in Arabidopsis thaliana siliques.

[0024] The present invention also provides a method for cultivating Arabidopsis thaliana with an increased number of silique seeds, comprising enhancing, increasing or upregulating the expression level of the gene encoding the protein described above in the target Arabidopsis thaliana, and / or the activity and / or content of the protein, to obtain Arabidopsis thaliana with an increased number of silique seeds, wherein the number of silique seeds in the Arabidopsis thaliana with an increased number of silique seeds is greater than that in the target Arabidopsis thaliana.

[0025] In the above method, enhancing, improving or upregulating the expression of the gene encoding the protein described above in Arabidopsis thaliana includes introducing the nucleic acid molecule described in c1), the expression cassette described in c2), or the recombinant vector described in c3) into the target Arabidopsis thaliana to obtain Arabidopsis thaliana with increased number of silique seeds.

[0026] The present invention also provides a method for cultivating Arabidopsis thaliana with a reduced number of siliques, comprising inhibiting, reducing or silencing the expression level of the gene encoding the protein described above in the target Arabidopsis thaliana, and / or the activity and / or content of the protein, to obtain Arabidopsis thaliana with a reduced number of siliques, wherein the number of siliques in the Arabidopsis thaliana is less than that in the target Arabidopsis thaliana.

[0027] The present invention also provides the described protein and / or the described biological material.

[0028] In this invention, the purpose of plant breeding may include cultivating Arabidopsis thaliana with an increased number of horned seeds.

[0029] In this invention, the Arabidopsis thaliana can be Arabidopsis thaliana Col-0 or Arabidopsis thaliana under the Col-0 background. spl7-1 Mutant.

[0030] In Arabidopsis spl7-1 In the mutant, the deletion of this gene reduces the number of seeds in the silique; therefore, for SPL7 Detailed studies of the functions can provide guidance for crop breeding work. Attached Figure Description

[0031] Figure 1 for SPL7 Identification of gene structure and T-DNA insertion site.

[0032] Figure 2 For mutant phenotypic identification. n=15, and significant differences were determined using two-tailed Student's test. t Tests: *: P < 0.05, **: P < 0.01, ***: P < 0.001, ns, non-significant.

[0033] Figure 3 pCAMBIA1300 -SPL7 Transformation spl7-1 T1 positive seedlings were identified by PCR.

[0034] Figure 4 for SPL7 Hap1 T1 phenotypic identification was performed with complementation. n=15, and significant differences were assessed using two-tailed Student's scores. tTests: *: P < 0.05, **: P < 0.01, ***: P < 0.001, ns, non-significant. Detailed Implementation

[0035] The present invention will now be described in further detail with reference to specific embodiments. The given embodiments are merely illustrative of the invention and not intended to limit its scope. The embodiments provided below can serve as a guide for further improvements by those skilled in the art and do not constitute a limitation on the invention in any way.

[0036] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials and reagents used in the following examples are commercially available.

[0037] Unless otherwise specified, the quantitative experiments in the following examples are all repeated three times, and the results are averaged.

[0038] The Arabidopsis Col-0 described in the following examples is documented in: Jin GT, Xu YC, Hou XH, Jiang J, LiXX, Xiao JH, Bian YT, Gong YB, Wang MY, Zhang ZQ, Zhang YE, Zhu WS, Liu YX, Guo YL*.2025. A de novo gene promotes seed germination under drought stress in Arabidopsis. Mol Biol Evol 42: msae262. The biological material is available to the public from the applicant and is intended solely for the purpose of replicating experiments of this invention and may not be used for any other purpose.

[0039] Arabidopsis thaliana under the Col-0 background in the following examples spl7-1 The mutant, kindly provided by Professor Liang Gang of the Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, is described in: Cai, Y.; Liang, G. CITF1 Functions Downstream of SPL7 to Specifically Regulate Cu Uptake in Arabidopsis. Int. J.Mol. Sci. 2022, 23, 7239. This biological material is available to the public from the applicant and is intended solely for the replication of experiments of this invention; it may not be used for any other purpose.

[0040] The Arabidopsis thaliana natural strains 5-15 in the following examples are described in: Zou YP, Hou XH, Wu Q, Chen JF, Li ZW, Han TS, Niu XM, Yang L, Xu YC, Zhang J, Zhang FM, Tan D, Tian Z, Gu H, Guo YL. Adaptation of Arabidopsis thaliana to the Yangtze River basin. Genome Biol. 2017 Dec 28;18(1):239. This biological material is available to the public from the applicant and is intended solely for the purpose of replicating experiments of this invention and may not be used for any other purpose.

[0041] The pCAMBIA1300 vector in the following examples has been described at: Hou XH, Xu YC, Sun TS, Gong YB, Li XT, Jin GT, Bian YT, Liu YN, Jiang J, Niu XM, Gu H*, Guo YL*. 2025. The Greenrevolution gene drives adaptation of Arabidopsis to the extremely highaltitude. Sci China Life Sci. doi:https: / / doi.org / 10.1007 / s11427-024-2769-x. This biological material is available to the public from the applicant and is intended solely for the purpose of replicating experiments of this invention; it may not be used for any other purpose.

[0042] The Agrobacterium GV3101 used in the following examples was purchased from Beijing Bomed Gene Technology Co., Ltd., with catalog number BC301-4-01.

[0043] The following examples used SPSS 11.5 statistical software to process the data. The experimental results are expressed as mean ± standard deviation, using a two-tailed Student's method. t The test results show that P < 0.05 (*) indicates a significant difference, P < 0.01 (**) indicates a highly significant difference, and P < 0.001 (***) indicates a highly significant difference.

[0044] Example 1 spl7-1 Phenotypic studies of homozygous mutants 1. spl7-1 Homozygous mutant verification Under Col-0 background spl7-1 mutants in SPL7There is a T-DNA insertion on the 5th intron ( Figure 1 PCR verification of the mutant was performed using a three-primer method. In wild-type Col-0, LP and RP could amplify bands, while... spl7-1 In mutants, BP on LP and T-DNA can amplify into bands, and the bands are single ( Figure 1 ).therefore, spl7-1 It is a homozygous mutant. Primers are as follows: LP: 5'-TTGGAAATTCAAGCTGATTCG-3'; RP: 5'-TCCACCTGTCAAAACCAAGAC-3'; BP: 5'-ATTTTGCCGATTTCGGAAC-3'.

[0045] 2. spl7-1 Phenotypic identification Wild type (WT) and spl7-1 Fifteen individual plants were selected from each plant. The 6th to 10th siliques from the bottom to the top of the main stem were taken from each plant. The siliques were dissected under a stereomicroscope, and the number of seeds and ovules in each silique were counted. The ratio of the number of seeds to the number of ovules was the seed set rate.

[0046] The results showed that, compared to WT, spl7-1 The number of seeds and ovules decreased; the number of seeds in WT was 56.04 ± 4.19. spl7-1 The number of seeds was 47.77 ± 1.57; the number of ovules in WT was 56.96 ± 4.31. spl7-1 The number of seeds was 48.48 ± 1.61. There was no significant difference in seed setting rate between the two groups. Therefore, SPL7 Regulating the number of seeds in Arabidopsis thaliana siliques ( Figure 2 ).

[0047] Example 2, in natural populations SPL7 Allele cloning and recombinant vector construction Found in the natural strain 5-15 of Arabidopsis thaliana from the Yangtze River population. SPL7 Dominant haplotypes of genes SPL7 Hap1 The haplotype was then transferred into the mutant for phenotypic identification.

[0048] Genomic DNA was extracted from Arabidopsis thaliana representative ecotypes 5-15, and then the haplotype was amplified using primers. SPL7 The full-length amplification primers are as follows: Forward primer: 5'-GCtctagaGCTCTCAATCGACAATCAGTAG-3'; Reverse primer: 5'-CGGcctgcaggCATGAACTAAACTGTACCTTCCG-3'.

[0049] SPL7 The coding sequence (CDS) of the gene in Arabidopsis thaliana strain 5-15 is SEQ ID No:2, encoding the SPL7 protein with the amino acid sequence SEQ ID No:1. The genomic gene encoding the SPL7 protein in the genomic DNA of Arabidopsis thaliana strain 5-15 is shown in SEQ ID No:3 of the sequence listing. In SEQ ID No:3, positions 1519-2069 are exon 1, positions 2466-2638 are exon 2, positions 2790-3016 are exon 3, positions 3090-3164 are exon 4, positions 3267-3374 are exon 5, positions 3691-3808 are exon 6, positions 3927-4062 are exon 7, positions 4288-4441 are exon 8, positions 4516-5076 are exon 9, and positions 5330-5632 are exon 10.

[0050] Using XbaI and SbfI endonucleases SPL7 Hap1 The haplotype fragment and the pCAMBIA1300 vector were double-digested with enzymes, and the digestion products were recovered by gel extraction. Subsequently, ligation was performed using T4 ligase, and the resulting cells were transformed into *E. coli* DH5α. First-generation sequencing was performed on PCR-positive colonies. After confirming the accuracy of the expression vector sequence, plasmids were extracted, successfully yielding the recombinant vector pCAMBIA1300-. SPL7 .

[0051] Recombinant vector pCAMBIA1300- SPL7 The structure is described as follows: A recombinant vector is obtained by inserting a DNA fragment with the sequence SEQ ID No:3 between the XbaI and SbfI restriction sites of the starting vector pCAMBIA1300, while keeping the other sequences of the vector pCAMBIA1300 unchanged. pCAMBIA1300- SPL7 The vector can express the SPL7 protein, whose amino acid sequence is SEQ ID No:1.

[0052] Subsequently, the recombinant vector pCAMBIA1300- SPL7Agrobacterium GV3101 was introduced, and mutants were introduced using the inflorescence infection method. spl7-1 .

[0053] The specific steps of the inflorescence infection method are as follows: 1) Select a single colony and culture it overnight, or use a frozen strain to transfer and culture it overnight at 28°C and 220 rpm.

[0054] 2) Expand to a 1:100 ratio and continue culturing overnight.

[0055] 3) Take the above bacterial solution and measure the OD. 600 The value is between 1.5 and 2.0.

[0056] 4) Centrifuge at 5000 rpm for 5-10 min.

[0057] 5) Before centrifugation, take a PCR tube and add 50 μL of water and 5 μL of bacterial culture to it for subsequent PCR identification.

[0058] 6) Prepare the infection solution by adding 0.2‰ of Silwett L-77.

[0059] 7) Resuspend the bacterial cells in an equal volume of infection solution and transfer them to a 100 mL Erlenmeyer flask.

[0060] 8) Cut off all the flowers of Arabidopsis thaliana that have grown to a suitable size, leaving only the flower buds.

[0061] 9) Lay the Arabidopsis thaliana horizontally so that the flower buds are immersed in the prepared solution. Use the bottom of the Erlenmeyer flask to shake the liquid surface from time to time. Use a gun to spot the flower buds that are not submerged.

[0062] 10) After one day of dark and moist culture, culture normally.

[0063] After harvesting the transformed T0 seeds, screening was performed using MS medium supplemented with 50 mg / L hygromycin B. Positive seedlings were then identified by PCR, and the amplification results are shown below. Figure 3 As shown, a total of 22 positive T1 strains were identified.

[0064] The PCR primers are as follows: Forward primer: 5'-CAGGAAACAGCTATGACC-3'; Reverse primer: 5'-GCTTCACAATCCGGAACCTGAC-3'.

[0065] SPL7 Hap1 It can restore the seed number and ovule number phenotypes of mutants, and SPL7 Hap1The number of seeds in the refilled siliques was 51.33±2.70, and the number of ovules was 52.29±3.02, both exceeding the average values ​​of the wild type (48.77±2.47; 50.11±1.49). Figure 4 In summary, SPL7 Regulating the number of seeds in Arabidopsis thaliana horns.

[0066] The present invention has been described in detail above. Those skilled in the art will recognize that the invention can be practiced in a wide range of ways with equivalent parameters, concentrations, and conditions without departing from its spirit and scope, and without requiring unnecessary experiments. While specific embodiments have been provided, it should be understood that further modifications can be made to the invention. In summary, according to the principles of the invention, this application is intended to include any changes, uses, or improvements to the invention, including changes made using conventional techniques known in the art that depart from the scope disclosed herein.

Claims

1. The use of a protein or a substance that regulates the expression of a gene encoding the protein or a substance that regulates the activity or content of the protein in any of the following; 1) Application in regulating the number of seeds in Arabidopsis thaliana siliques; 2) Application in the preparation of products that regulate the number of seeds in Arabidopsis thaliana siliques; 3) Application in cultivating Arabidopsis thaliana with altered seed number in siliques; 4) Application in the preparation of Arabidopsis products with altered seed number in siliques; 5) Application in Arabidopsis thaliana breeding; The protein is any of the following proteins: a1) A protein with the amino acid sequence SEQ ID No:1; a2) A protein having the same function as the amino acid sequence shown in SEQ ID No:1, but with one or more amino acid residues substituted and / or deleted and / or added. a3) Proteins that share more than 80% identity with the amino acid sequence defined by a1) or a2) and have the same function; a4) A fusion protein obtained by attaching a tag to the end of any of the proteins defined in a1)-a3).

2. The application according to claim 1, characterized in that, The substance that regulates gene expression or the substance that regulates the activity or content of the protein is a biological material related to the protein in the application of claim 1, and the biological material is any one of the following: c1) The nucleic acid molecule encoding the protein; c2) An expression cassette containing the nucleic acid molecule described in c1); c3) A recombinant vector containing the nucleic acid molecule described in c1), or a recombinant vector containing the expression cassette described in c2); c4) Recombinant microorganisms containing the nucleic acid molecules described in c1), or recombinant microorganisms containing the expression cassette described in c2), or recombinant microorganisms containing the recombinant vector described in c3); c5) A transgenic plant cell line containing the nucleic acid molecule described in c1), or a transgenic plant cell line containing the expression cassette described in c2); c6) Transgenic plant tissue containing the nucleic acid molecules described in c1), or transgenic plant tissue containing the expression cassette described in c2); c7) A transgenic plant organ containing the nucleic acid molecule described in c1), or a transgenic plant organ containing the expression cassette described in c2).

3. The application according to claim 3, characterized in that, c1) The nucleic acid molecule is any of the following DNA molecules. d1) The nucleotide sequence is the DNA molecule shown in SEQ ID No:3; d2) The coding sequence is the DNA molecule shown in SEQ ID No:2; d3) has 90% or more identity with the nucleotide sequence defined by d1) or d2) and is a DNA molecule encoding the protein of claim 1; d4) Hybridizes under stringent conditions to a nucleotide sequence defined by d1) or d2) and encodes a DNA molecule that encodes the protein of claim 1.

4. A method for changing the number of seeds in a Arabidopsis thaliana silique, characterized in that: The method includes steps P or M, wherein step P is to enhance, increase, or upregulate the activity and / or content of the protein of claim 1 in the target Arabidopsis thaliana, or / and enhance, increase, or upregulate the expression level of the gene encoding the protein of claim 1, to increase the number of Arabidopsis thaliana pod seeds; and step M is to inhibit, decrease, or silence the activity and / or content of the protein of claim 1 or 2 in the target Arabidopsis thaliana, or / and inhibit, decrease, or downregulate the expression level of the gene encoding the protein of claim 1, to reduce the number of Arabidopsis thaliana pod seeds.

5. A method for cultivating Arabidopsis thaliana with an increased number of seeds per silique, characterized in that, This includes enhancing, increasing, or upregulating the expression level of the gene encoding the protein of claim 1 in the target Arabidopsis, and / or the activity and / or content of the protein, to obtain Arabidopsis with an increased number of silique seeds, wherein the Arabidopsis with the increased number of silique seeds has more silique seeds than the target Arabidopsis.

6. The method according to claim 5, characterized in that, The enhancement, improvement, or upregulation of the expression of the gene encoding the protein of claim 1 in Arabidopsis thaliana comprises introducing the nucleic acid molecule of claim 2 (c1), the expression cassette of claim 2 (c2), or the recombinant vector of claim 2 (c3) into the target Arabidopsis thaliana to obtain Arabidopsis thaliana with increased number of silique seeds.

7. A method for cultivating Arabidopsis thaliana with a reduced number of seeds per silique, characterized in that, Including inhibiting, reducing, or silencing the expression level of the gene encoding the protein of claim 1 in the target Arabidopsis, and / or the activity and / or content of the protein, to obtain Arabidopsis with a reduced number of silique seeds, wherein the number of silique seeds in the Arabidopsis with a reduced number of silique seeds is less than that in the target Arabidopsis.

8. The protein of claim 1 and / or the biomaterial of claim 2 or 3.

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