Antibacterial peptide and plant polyphenol complex-containing capsule and antibacterial liquid composition

Abstract

The application discloses a kind of containing antibacterial peptide and plant polyphenol complex capsule, by mass fraction, including active ingredient 30~35 parts, microcapsule wall material 55~60 parts, PEG-40 hydrogenated castor oil emulsion 5~10 parts, the active ingredient includes antibacterial peptide 15-30 parts, plant polyphenol 50-60 parts, phenoxyethanol 0.5~1.0 parts, citric acid or sodium citrate 3~5 parts and water, and the mass ratio of water in active ingredient is 10%~20%, the molecular weight of antibacterial peptide is 2000~7000, and the molecular weight of plant polyphenol is 500~3000.The application also discloses including the above-mentioned containing antibacterial peptide and plant polyphenol complex capsule.The application also discloses the bacteriostatic composition including the above-mentioned complex capsule.

Description

Technical Field

[0001] This invention relates to the field of daily chemical detergents, and more particularly to a capsule and liquid composition containing an antimicrobial peptide and a plant polyphenol complex. Background Technology

[0002] With the increasing health awareness of the public, consumers' demand for laundry products has shifted from basic stain removal to multi-functional needs such as green safety, long-lasting antibacterial effect, and odor removal. In the rainy season in southern my country, due to high humidity, clothes often develop odors after drying. The main reason for these odors is the secondary growth of microorganisms. Conventional washing only removes most stains and microorganisms from the surface of the clothes. The remaining microorganisms will rapidly multiply and metabolize in high-humidity environments. For example, *Moraxella osloensis* can survive in damp environments such as clothing and kitchen sponges during the rainy season, and its metabolic product, 4-methyl-3-hexenoic acid, is a major source of odor in air-dried clothes.

[0003] Currently, mainstream antibacterial laundry products on the market still have significant technological shortcomings, making it difficult to meet the dual demands of long-lasting antibacterial effect and safe washing and care. On the one hand, traditional chemically synthesized antibacterial agents are easily washed away in large quantities with water during the washing process, resulting in a low deposition rate on fabrics and failing to achieve long-lasting antibacterial effect on washed clothes. On the other hand, the chemical antibacterial components remaining on clothing have poor biocompatibility, and long-term contact with human skin can easily cause irritation, posing safety risks. Natural antibacterial ingredients, such as antimicrobial peptides and plant polyphenols, have high biocompatibility, antioxidant and antibacterial properties, low irritation, and are easily degradable, perfectly aligning with the research and development concept of green detergents. However, in existing technologies, antimicrobial peptides and plant polyphenols are difficult to apply directly to liquid detergents due to compatibility issues: antimicrobial peptides have poor stability in water-based liquid detergents, and are prone to molecular structure changes, enzymatic failure, aggregation, and precipitation during storage and use; plant polyphenols contain a large number of phenolic hydroxyl structures, making them chemically unstable and easily oxidized and deteriorated in the acid-base system of liquid detergents or at room temperature, losing their antibacterial activity; antimicrobial peptides and plant polyphenols have poor compatibility with surfactants in liquid detergents, and are prone to stratification and precipitation during long-term storage, resulting in a reduction in active ingredients. This not only fails to guarantee the antibacterial stability of the product during its shelf life, but also makes it difficult to exert long-lasting antibacterial and deodorizing effects throughout the entire process of washing, drying, and wearing clothes. Summary of the Invention

[0004] To address the aforementioned problems, this invention proposes a capsule containing an antimicrobial peptide and a plant polyphenol complex. The antimicrobial peptide and plant polyphenol are compounded in a specific ratio and then encapsulated using microcapsules to obtain microcapsule particles containing the antimicrobial peptide and plant polyphenol complex. These microcapsule particles, after pre-dispersion, can be directly applied to liquid detergents and, when compounded with surfactants and functional additives, produce a long-lasting antibacterial liquid composition.

[0005] The technical solution adopted in this invention is as follows: A capsule containing an antimicrobial peptide and plant polyphenol complex comprises, by weight, 30-35 parts of active ingredients, 55-60 parts of microcapsule wall material, and 5-10 parts of PEG-40 hydrogenated castor oil emulsion. The active ingredients include 1-30 parts of antimicrobial peptide, 50-60 parts of plant polyphenol, 0.5-1.0 parts of phenoxyethanol, 3-5 parts of citric acid or sodium citrate, and water. The water content in the active ingredients is 10%-20% by weight. The molecular weight of the antimicrobial peptide is 2000-7000, and the molecular weight of the plant polyphenol is 500-3000.

[0006] In this complex capsule, antimicrobial peptides are a class of basic polypeptides with antimicrobial activity, with a molecular weight of 2000-7000, composed of 20-60 amino acid residues, and possess broad-spectrum antimicrobial activity. Plant polyphenols can penetrate into the microbial cell structure, affecting the integrity of the cell structure and thus influencing microbial metabolism. The inhibitory effects of plant polyphenols from different sources on microorganisms vary considerably, with overall inhibitory effects on Gram-negative bacteria being better than on Gram-positive bacteria. Antimicrobial peptides have specific microbial targets and can directly inhibit specific microorganisms; however, due to their poor permeability into cell structures, their inhibitory levels against some highly resistant Gram-positive bacteria are not high. In this formulation, by combining antimicrobial peptides and plant polyphenols, when the ratio of the two is (0.3-0.5):1, the synergistic antibacterial effect is relatively good, showing good inhibitory effects against Gram-positive bacteria (such as Escherichia coli) and also against Moraxella osloi, effectively removing the odor of dried goods during the rainy season. The reason is that when plant polyphenols and antimicrobial peptides are combined in a specific ratio, the plant polyphenols improve the penetration behavior of antimicrobial peptides into microbial cell structures, thereby enhancing the adhesion of antimicrobial peptides to microbial protein membranes, synergistically increasing their effect and enhancing the broad spectrum of antibacterial activity.

[0007] This method utilizes encapsulation technology to encapsulate the active ingredient, effectively isolating it from surfactants and oxygen in the liquid detergent and maintaining its structural stability during storage. Accelerated stability experiments were conducted, storing samples at 40°C for 3 months before testing their antibacterial rate, simulating changes in the product's antibacterial effect over its 3-year shelf life. Results showed that samples with added active ingredient capsules exhibited an antibacterial rate >99%, while samples with directly added active ingredients showed an antibacterial rate <50%. Encapsulation technology improved the stability of the active ingredient within the system.

[0008] In summary, this solution enhances the broad-spectrum antibacterial properties of natural antibacterial ingredients by combining antimicrobial peptides and plant polyphenols. Furthermore, the use of encapsulation technology ensures the long-term stability of the active ingredients in the water-based liquid composition, solving the technical challenges of stability and compatibility of these natural antibacterial ingredients. Modification of the capsule wall material increases the deposition of active ingredients on the fabric during washing, improving long-lasting antibacterial performance and alleviating the technical problem of unpleasant odors in dried clothes during the rainy season. The use of natural antibacterial ingredients ensures that the residue on intimate apparel is biocompatible with the human body, minimizing the risk of allergies, thus fulfilling the dual requirements of long-lasting antibacterial effect and safe washing and care.

[0009] Optionally, the antimicrobial peptide includes one or two of plant antimicrobial polypeptides or nisin, and the plant polyphenols include one or more of tea polyphenols, camellia seed polyphenols, or pomegranate peel polyphenols.

[0010] Optionally, the microcapsule wall material includes one or more of sodium alginate, gelatin, cyclodextrin, starch and its modified compounds, or chitosan and its modified compounds.

[0011] Optionally, the microcapsule wall material is a biodegradable chitosan modified by cationic quaternization.

[0012] Using cationic quaternization-modified microcapsule wall materials can increase the deposition of active ingredient microcapsules on fabrics.

[0013] An antibacterial liquid composition, characterized in that it comprises 0.2-0.3 parts of the complex capsule shown above, 10-16 parts of sodium fatty alcohol polyoxyethylene ether sulfate, 5-9 parts of sodium linear alkylbenzene sulfonate, 2-5 parts of fatty alcohol polyoxyethylene ether, 1-5 parts of alkyl glycoside, 0.5-1 part of coconut oil fatty acid soap, 0.1-0.5 parts of enzyme preparation, 0.1-0.5 parts of chelating agent, 0.2-4.3 parts of viscosity modifier, 0.1-0.3 parts of pH buffer, 0.1-0.2 parts of preservative, and water.

[0014] Among the above-mentioned laundry detergent components, sodium fatty alcohol polyoxyethylene ether sulfate (AES), sodium linear alkylbenzene sulfonate, fatty alcohol polyoxyethylene ether (AEO-9), alkyl glycoside (APG), and coconut oil fatty acid soap are surfactants, all of which are conventional commercially available products. They can reduce the interfacial tension at the interface between the two phases, thereby improving foaming and cleaning and stain removal.

[0015] Enzyme preparations enhance the removal of stains such as protein. The complex capsules have antibacterial properties. The complex capsules are pre-dispersed with water at a ratio of 3:1 to 5:1 (pure water:capsule). Stir at high speed for 5 to 10 minutes. The dispersion can be observed under a microscope; the absence of obvious particles indicates uniform dispersion. It can be added to the system when the temperature is below 40°C.

[0016] Chelating agents can bind with metal ions, reduce the content of free metal ions, soften water quality, and reduce the probability of binding between sensitive components and metal ions in the system, thus preventing the deterioration of sensitive components.

[0017] Optionally, the chelating agent includes one or both of tetrasodium glutamate diacetate (GLDA-4Na) and disodium ethylenediaminetetraacetate (EDTA-2Na).

[0018] Optionally, the viscosity modifier includes one or both of sodium chloride and sodium sulfate.

[0019] Optionally, the pH buffer comprises a combination of two or three of citric acid, sodium citrate, and liquid alkali.

[0020] Optionally, the preservative is two or more of the following: methylisothiazolinone (MIT), methylchloroisothiazolinone (CMIT), benzisothiazolinone (BIT), phenoxyethanol, sodium benzoate, or potassium sorbate.

[0021] The beneficial effects of this invention are as follows: by compounding antimicrobial peptides and plant polyphenols, the broad-spectrum antimicrobial performance of natural antimicrobial ingredients is enhanced. Furthermore, by utilizing encapsulation technology, the antimicrobial peptides and plant polyphenols can maintain the long-term stability of active ingredients in water-based liquid compositions, thus solving the technical problems of stability and compatibility of natural antimicrobial ingredients such as antimicrobial peptides and plant polyphenols. Moreover, by modifying the capsule wall material, the amount of active ingredients deposited on the fabric is increased during the washing process, improving the long-lasting antimicrobial performance and alleviating the technical problem of odors in clothes dried during the rainy season. The use of natural antimicrobial ingredients ensures that the residue on intimate apparel is biocompatible with the human body and is less likely to cause allergies, thus fulfilling the dual needs of long-lasting antimicrobial effect and safe washing and care. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0023] Figure 1 This is a simplified structural diagram of a complex capsule preparation device; Figure 2 This is a diagram showing the positional relationship between the collection bucket and the outer bucket; Figure 3 yes Figure 2 A simplified cross-sectional diagram along the AA direction.

[0024] The attached figures are labeled as follows: 1. Inlet fan; 2. Feed hopper; 3. Outer barrel; 4. Spray pipe; 5. Collection barrel; 501. Air extraction port; 6. Base; 7. Stainless steel mesh plate; 8. Porous filter mesh plate; 9. Temperature control pipe; 10. Inner cylinder; 11. Guide cone; 12. Air distribution hood; 13. Return pipe. Detailed Implementation

[0025] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0026] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0027] First, capsules containing antimicrobial peptides and plant polyphenols were prepared. 15-30 parts of antimicrobial peptides, 50-60 parts of plant polyphenols, 0.5-1.0 parts of phenoxyethanol, and 3-5 parts of sodium citrate were dissolved in water and then solidified to obtain an active ingredient with a water content of 10%-20%. Then, the active ingredient particles, microcapsule wall material, and PEG-40 hydrogenated castor oil emulsion were mixed to prepare the complex capsules. The particle size of the complex capsules was 10-20 μm, and the content of the active ingredient was 30-35 parts, the content of the microcapsule wall material was 55-60 parts, and the content of the PEG-40 hydrogenated castor oil emulsion was 5-10 parts.

[0028] The following abbreviations will be used in the following content and have the functions indicated therein.

[0029] LAS: Sodium linear alkylbenzene sulfonate, carbon chain length 12-14, purity 96%. AES: Sodium fatty alcohol polyoxyethylene ether sulfate, the carbon chain length of the fatty alcohol is 12-14, the average EO number is 2, and the purity is 70%.

[0030] Coconut oil fatty acids: The carbon chain length of coconut oil ranges from 8 to 18. AEO-9: Fatty alcohol polyoxyethylene ether, the carbon chain length of the fatty alcohol is 12-14, and the average number of EOs is 9; Alkyl glycoside, abbreviated as APG, with a purity of 50%, is a green surfactant.

[0031] Unless otherwise specified, the raw materials and reagents used in the following examples are all commercially available standard products.

[0032] pH: refers to the test temperature of 25℃. The sample solution is prepared with freshly boiled and cooled distilled water, and the mass concentration of the sample solution is 10%. The solution is measured after mixing.

[0033] Product stability: Refer to QB / T 1224 Liquid Detergents for Clothing, test the heat resistance and cold resistance of the samples. After the samples are placed at (40±2)℃ and (-5±2)℃ for 24 h respectively, observe the appearance of the samples after they are restored to room temperature. If layering, precipitation or sedimentation occurs, it is recorded as "unqualified". If the appearance is uniform and stable, and there is no layering, precipitation or sedimentation, it is recorded as "qualified".

[0034] Antibacterial efficacy test: Following QB / T 2738-2023 "Evaluation Method for Antibacterial and Antimicrobial Efficacy of Daily Chemical Products", strain culture and screening were conducted, and the suspension quantitative method was used for determination. The evaluation and calculation method for the antibacterial rate was adopted. Specifically: action time 5 min, test concentration, 10% sample dilution solution; Long-term antibacterial effect test: Following T / ZGXX 004, a washed cotton cloth was used, the test concentration was 0.2% sample dilution solution, and the experimental strains were Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC 6538), Candida albicans (ATCC10231), and Moraxella osloi (ATCC 10973).

[0035] Odor Removal: The odor removal effect was evaluated after testing clothing with an odor that had been air-dried. Regular clothing was also tested simultaneously. After air-drying, the odor was evaluated. The test clothing consisted of pure cotton T-shirts. The process involved placing the odor-affected clothing and other clothes being washed together into the washing machine, adding 84g of the test sample, selecting a common washing mode, 42L of water, 2 rinses, and a 7-minute spin cycle. After washing, the clothes were removed and air-dried indoors (during Hangzhou's rainy season). Five evaluators then assessed the odor of the washed clothes. If all evaluators agreed that there was no odor residue on a particular type of clothing, the product was considered to have an "odor removal effect" for that odor; otherwise, it was considered "ineffective."

[0036] In the formulation examples, a laundry detergent base was prepared without fragrance or antibacterial ingredients, and the pH was adjusted to 6.0-8.0 to obtain laundry detergent base 1. Complex capsules were added to laundry detergent base 1 at concentrations of 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%, respectively, to obtain Examples 1, 2, 3, 4, and 5. Laundry detergent base 1 without complex capsules was Comparative Example 1. Adding 0.5% antimicrobial peptides to laundry detergent base 1 yielded Comparative Example 2. Adding 0.5% plant polyphenols to laundry detergent base 1 yielded Comparative Example 3. The samples obtained from the above examples and comparative examples were dried in a 40°C oven for 3 months (12 weeks), then removed to ambient temperature. The product appearance was observed, and the antibacterial, long-lasting antibacterial, and odor removal performances were tested. The results are shown in Table 1.

[0037] Examples 1-6, in comparison, revealed that: 0.1% of the complex capsules did not show good antibacterial effect against Gram-negative bacteria and Gram-positive bacteria with unique cell structures, failing to achieve the expected results. When the complex capsule concentration exceeded 0.3%, the capsules tended to aggregate, exhibiting poor stability within the matrix and increasing costs. Therefore, a concentration of 0.2-0.3 parts of complex capsules in the liquid composition achieved the desired antibacterial effect and odor removal.

[0038] Comparing Examples 1-3, it was found that without the addition of the complex capsules, or with the addition of antimicrobial peptides alone, or with the addition of plant polyphenols alone, even with the dosage increased to 0.5%, the expected antibacterial and odor-removing effects could not be achieved. It is speculated that the instability within the matrix is ​​poor, leading to the inactivation of the antibacterial components.

[0039] This embodiment further discloses an apparatus for preparing a complex capsule. The traditional method for preparing microcapsules involves mixing active ingredients, wall materials, and emulsions, followed by drying with hot air. However, the hot air drying process can easily cause the active ingredients to degrade and become ineffective. In order to prepare complex capsules with better and longer-lasting antibacterial effects, this embodiment further discloses the following preparation apparatus.

[0040] It should be noted that the appendix Figure 3 The half-arrow indicates the direction of emulsion flow, while the full arrow indicates the direction of airflow.

[0041] Combined with the following appendix Figure 1 Appendix Figure 2 and appendix Figure 3As shown, the system includes an outer barrel 3, a collection barrel 5 detachably installed at the opening of the outer barrel 3, an air extraction port 501 connected to the barrel wall of the collection barrel 5, an air intake fan 1 installed at one end of the outer barrel 3, and a feed hopper 2 installed at one end of the air intake fan 1. An inner cylinder 10 is provided inside the outer barrel 3, and one end of the inner cylinder 10 is detachably sealed to the opening of the collection barrel 5. Two stainless steel mesh plates 7 are arranged and installed on the opening of the collection barrel 5, and one end of the stainless steel mesh plate 7 is connected to a semiconductor cooling chip. The semiconductor cooling chip continuously cools the stainless steel mesh plate 7, maintaining the temperature of the stainless steel mesh plate 7 at 5~10 degrees Celsius. Five porous filter plates are arranged and distributed in the inner cylinder 10. The porous filter plates are parallel and non-contacting. The mesh diameter of the porous filter plates is larger than that of the stainless steel mesh plate 7. An air distribution hood 12 is installed at the bottom of the outer barrel 3, and the hood 12 is located inside the outer barrel 3. A guide cone 11 is installed at the other end of the inner barrel 10, and the guide cone 11 is located between the air distribution hood 12 and the inner barrel 10. Liquid collection tanks are distributed around the perimeter of the air distribution hood 12, and return pipes 13 are connected to the liquid collection tanks. Temperature control pipes 9 are spirally wound on the outer wall of the inner barrel 10. Liquid with a temperature of 20-25 degrees Celsius flows through the temperature control pipes 9. The function of the temperature control pipes 9 is to maintain the temperature of the inner barrel 10 wall and the porous filter plates below 25 degrees Celsius. Spray pipes 4 are interspersed on the outer barrel 3 and the inner barrel 10, and the spray pipes are located between the stainless steel mesh plate 7 and the porous filter plates. The method of using this device is as follows: First, the microcapsule wall material is dissolved in the emulsion. The spray pipe 4 sprays the emulsion containing the dissolved microcapsule wall material onto the porous filter plate. Then, the emulsion containing the microcapsule wall material flows continuously from top to bottom. During the above process, active ingredient particles are added to the feed hopper 2. The air intake fan 1 located outside the outer barrel 3 continuously blows the airflow carrying the active ingredient particles from the feed hopper 2 into the outer barrel 3. The airflow carrying the active ingredient particles passes through the base 6 and then through the air distribution hood 12, continuously flowing upward from bottom to top. At this time, the air pump connected to the air extraction port 501 starts to draw negative pressure into the collection barrel 5. After the airflow and liquid flow mix, a portion of the liquid will flow with the liquid. The airflow is blown onto the stainless steel mesh plate 7 and trapped by it (the trapped particles are a mixture of active particles, capsule wall material, and emulsion). Since the temperature of the stainless steel mesh plate 7 is only 5~10 degrees Celsius, the particles can be trapped and attached at a relatively low temperature. Meanwhile, the external vacuum pump is in a negative pressure state on the collection tank 5, and the emulsion continues to evaporate. When the emulsion evaporates to the point that the trapped particles are solid, the preparation of the capsule particles is completed. After completion, the collection tank 5 is disassembled and the tank wall is tapped to make the microcapsule particles fall off. The complex capsules prepared using this device are prepared under low temperature conditions throughout the process, avoiding the degradation of active substances caused by high temperature.

[0042] In this device, the emulsion flowing down from the bottom is guided by the guide cone 11 and then flows to the collection tank, and is finally recycled and reused by the return pipe 13.

[0043] The above-described embodiments only illustrate some aspects of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A capsule containing an antimicrobial peptide and a plant polyphenol complex, characterized in that, The product comprises, by weight, 30-35 parts of active ingredient, 55-60 parts of microcapsule wall material, and 5-10 parts of PEG-40 hydrogenated castor oil emulsion. The active ingredient includes 15-30 parts of antimicrobial peptide, 50-60 parts of plant polyphenols, 0.5-1.0 parts of phenoxyethanol, 3-5 parts of citric acid or sodium citrate, and water. The water content in the active ingredient is 10%-20% by weight. The molecular weight of the antimicrobial peptide is 2000-7000, and the molecular weight of the plant polyphenol is 500-3000.

2. The capsule containing an antimicrobial peptide and plant polyphenol complex according to claim 1, characterized in that, The antimicrobial peptide includes one or two of plant antimicrobial polypeptides or lactic acid nisin, and the plant polyphenols include one or more of tea polyphenols, tea seed oil polyphenols, or pomegranate peel polyphenols.

3. A capsule containing an antimicrobial peptide and plant polyphenol complex according to claim 1, characterized in that, Microcapsule wall materials include one or more of the following: biodegradable materials such as sodium alginate, gelatin, cyclodextrin, starch and its modified compounds, or chitosan and its modified compounds.

4. A capsule containing an antimicrobial peptide and plant polyphenol complex according to claim 1, characterized in that, The microcapsule wall material is a biodegradable chitosan modified by cationic quaternization.

5. An antibacterial liquid composition, characterized in that, The product, by mass fraction, comprises 0.2-0.3 parts of the complex capsule as described in any one of claims 1-4, 10-16 parts of sodium fatty alcohol polyoxyethylene ether sulfate, 5-9 parts of sodium linear alkylbenzene sulfonate, 2-5 parts of fatty alcohol polyoxyethylene ether, 1-5 parts of alkyl glycoside, 0.5-1 part of coconut oil fatty acid soap, 0.1-0.5 parts of enzyme preparation, 0.1-0.5 parts of chelating agent, 0.2-4.3 parts of viscosity modifier, 0.1-0.3 parts of pH buffer, 0.1-0.2 parts of preservative, and water.

6. The antibacterial liquid composition according to claim 5, characterized in that, The chelating agent includes one or both of tetrasodium glutamate diacetate and disodium ethylenediaminetetraacetate.

7. The antibacterial liquid composition according to claim 5, characterized in that, The viscosity modifier includes one or both of sodium chloride and sodium sulfate.

8. The antibacterial liquid composition according to claim 5, characterized in that, The pH buffer comprises two or three of the following: citric acid, sodium citrate, and liquid alkali.

9. The antibacterial liquid composition according to claim 5, characterized in that, The preservative is a combination of two or more of the following: methylisothiazolinone, methylchloroisothiazolinone, benzisothiazolinone, phenoxyethanol, sodium benzoate, or potassium sorbate.

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