A device and method for in-situ blocking and sampling of avian influenza virus from migratory birds in a polar strong wind environment

Abstract

The application discloses a kind of migratory bird source avian influenza virus in-situ closure sampling device and method under polar strong wind environment, belong to the field of animal virus field sampling technology.Cubic inflatable main cavity made of low-temperature resistant composite film is main body, equipped with one-way inflation valve, top bacteria removal filter, side sealing filling window, integrated operating glove;Further add two ends sealed extension disinfection channel and built-in carcass sealing recovery bag;Further match operating glove anti-cutting sensing component, cavity built-in camera auxiliary component.In the premise that carcass is in situ immobile, personnel is upwind station, sequentially complete consumable preloading, carcass enters cabin, inflation sealing, cavity sampling, sample disinfection discharge, pollutant packaging, cavity sterilization exhaust and whole recovery whole process, realize aerosol, pollutant zero diffusion, biological safety is high, sampling is accurate, device can be reused, suitable for antarctic and other polar regions migratory bird, penguin high pathogenic virus in-situ sampling operation.

Description

Technical Field

[0001] This invention belongs to the field of animal virus field sampling technology, specifically involving an in-situ sealed bagging sampling device and method for animal carcasses with disease and death, which is suitable for the extreme winds, clean ecological protection, and high biosafety risks in polar regions. It is especially applicable to field sampling operations for highly pathogenic avian influenza viruses in polar regions such as Antarctica, with no spread and zero pollution. Background Technology

[0002] Highly pathogenic avian influenza (HPAIV) can be transmitted via aerosols over both short and long distances, and is one of the most common modes of transmission in farms and wild bird populations. Dissection and tissue cutting of dead migratory birds generate large amounts of virus-containing droplets and microparticles, forming highly infectious aerosols. Studies have confirmed that HPAIV aerosols can remain stable in the environment for extended periods and can spread over long distances. Under typical farm conditions, virus particles can be detected even after spreading to distances exceeding 40 meters, and can persist in the air for up to 15 days after the host ceases shedding the virus. This results in widespread contamination, extremely high concealment, and immense difficulty in prevention and control (US Centers for Disease Control and Prevention (CDC). Avian influenza virus transmission [EB / OL]. 2025. 2026-05-07. https: / / www.cdc.gov / bird-flu / virus-transmission / index.html.).

[0003] In the harsh polar winds, the risk of aerosol diffusion is amplified exponentially. Antarctica is frequently accompanied by sudden, strong gusts of wind with high speeds, high force, and no shelter. When sampling migratory birds, their dense feathers and downy fluff are easily shed. Viral aerosols, infected feathers, and tissue debris from autopsies can spread rapidly and over long distances driven by strong winds. This not only directly poses a high risk of infection for sampling personnel but also causes irreversible and devastating pollution to the pristine Antarctic ecosystem, which is free of native avian influenza viruses. This seriously threatens the safety of native polar bird and mammal populations and violates the red line for ecological protection in polar scientific research.

[0004] Meanwhile, the lack of biosafety cabinets, negative pressure chambers, and on-site disinfection equipment in polar fields makes it impossible to control aerosol diffusion through conventional means. Existing sampling techniques only provide basic contamination prevention and do not address the risk of easy diffusion of avian influenza aerosols in strong polar winds, posing significant safety and ecological vulnerabilities. Conventional inflatable sealed dissection gloves are mostly used in ambient temperatures or ordinary field scenarios. Polar operations require wearing double-layered, thickened, cold-proof, and puncture-resistant protective gloves, which significantly reduces tactile sensitivity. During dissection, it is extremely easy to accidentally cut the one-piece sealed glove. Once the glove is damaged, the sealed system fails, directly causing viral aerosol leakage, posing a significant biosafety hazard. In addition, the fully enclosed cavity has blind spots. Polar scientific expeditions are mostly conducted by single personnel without external assistance, making dissection positioning and standardized sampling difficult, and the standardization of operations and sample quality are hard to guarantee.

[0005] Therefore, developing a polar-specific in-situ closed sampling method that blocks aerosol generation and diffusion at the source and possesses proactive safety warning and visualization assistance functions has become a core necessity for ensuring the safety of polar pathogen monitoring. Developing a bagging sampling method that is polar-specific, in-situ closed, wind-resistant, zero-ecological-pollution, highly biosafe, and equipped with intelligent assistance functions is of significant practical necessity and technological innovation.

[0006] To address this, the present invention employs a puncture-resistant, low-temperature-resistant, and highly transparent sealed sampling bag, which completely encloses the carcass of diseased and dead birds from top to bottom, forming a completely sealed operating chamber. Sampling personnel hold the carcass in place outside the bag with both hands, while dissecting instruments operate within the sealed, windless space inside the bag, allowing for low-disturbance, small-amplitude, targeted shearing of tissues such as the lungs, brain, and intestines, thus inhibiting aerosol generation at the source. Sampling tubes containing RNAlater protective fluid directly receive the tissues inside the bag, without opening the bag, exposing the carcass, or allowing direct airflow to the outside environment, completely blocking the spread of viral aerosols and infected feathers under strong polar winds. Simultaneously, a cut-resistant sensor warning structure and a built-in camera auxiliary structure are added to address the drawbacks of poor tactile feedback from thick gloves and limited operational visibility, achieving safe polar sampling with zero aerosols, zero diffusion, and zero ecological pollution. Summary of the Invention

[0007] The purpose of this invention is to overcome the shortcomings of existing technologies and provide an in-situ sealed sampling device and method for avian influenza virus from migratory birds in polar strong wind environments. By keeping the virus in place, bagging the entire sample, operating in a fully enclosed manner, sampling inside the bag, and sealing all waste, the invention blocks the spread of virus aerosols and virus-carrying feathers in polar strong winds from the source. At the same time, it meets the safe sampling requirements in polar fields where there is no equipment or laboratory, and achieves the triple goals of personnel safety, sample validity, and zero ecological pollution.

[0008] An in-situ sealed sampling device for avian influenza virus from migratory birds in polar strong wind environments includes a cubic inflatable main cavity. The cubic inflatable main cavity is made of a composite membrane that is resistant to low temperatures of -30℃, puncture-proof, highly airtight, and highly transparent. A one-way inflation valve is provided on one side of the cavity, forming a rigid cubic operating space after inflation. A 0.22μm virus-grade high-efficiency sterilization filter is provided at the top of the cubic inflatable main cavity. A zippered, sealable filling window is provided on the side of the cavity, and a bottom window is provided on the bottom surface of the cavity. A pair of operating gloves are integrally sealed on the front of the cavity, and the inside of the operating gloves is connected to the cavity.

[0009] Furthermore, it also includes an extended disinfection channel; the extended disinfection channel is a straight tube with independent sealing structures at both ends. The proximal end of the extended disinfection channel is sealed and connected to the cubic inflatable main cavity, and the distal end is an external outlet. The channel is pre-filled with disinfectant.

[0010] Furthermore, the cube-shaped inflatable main cavity is equipped with an independent sealed recycling bag.

[0011] Furthermore, it also includes a cut-resistant early warning sensing component for operating gloves; a low-temperature resistant flexible cut-resistant sensing array is arranged inside the interlayer of the operating glove, and the sensing circuit is embedded in the membrane layer of the operating glove and does not penetrate the sealing wall; the sampling device is equipped with a low-temperature audible and visual vibration alarm terminal, and the alarm terminal is connected to the cut-resistant sensing array of the operating glove via signal connection.

[0012] Furthermore, the cutting sensor array and the low-temperature audible and visual vibration alarm terminal are resistant to operating temperatures as low as -30°C.

[0013] Furthermore, it also includes a built-in camera auxiliary component; a low-temperature resistant wide-angle miniature camera is embedded in the inner top of the cubic inflatable main cavity, and the base of the wide-angle miniature camera is heat-sealed with the cavity membrane; the sampling device is equipped with a wireless image transmission module and a low-temperature display terminal.

[0014] Furthermore, the wide-angle miniature camera lens is equipped with an anti-fog coating, and the sampling device is also equipped with a video storage module; the wide-angle miniature camera, wireless image transmission module, low-temperature display terminal, and video storage module are all resistant to a low-temperature environment of -30℃.

[0015] A method for in-situ sealing and sampling of migratory bird-borne viruses in polar high-wind environments, based on the above sampling device, includes the following steps: 1) Pre-installation of consumables and window sealing Through the loading window on the side of the sampling device, sterile dissecting scissors, sterile forceps, sterile sampling tubes containing RNAlater protective solution, and disinfectant are loaded into the cubic inflatable main cavity. After the consumables are loaded, the loading window on the side is completely sealed to put the device in a closed initial state. 2) In-situ loading of the body and sealing of the entire compartment After discovering the carcass of a dead migratory bird or penguin, keep the carcass in its original position and posture to avoid disturbing it and causing the spread of aerosols and feathers. The sampling personnel should stand upwind of the carcass, open the bottom window, and place the carcass steadily into the cube-shaped inflatable main cavity. Then, completely seal the bottom window to make the entire cavity a leak-free, fully enclosed structure. 3) Inflatable molding constructs a rigid operating space Clean air is injected into the cavity through the one-way inflation valve on the cubic main inflatable cavity, causing the cavity to expand into a standard cubic rigid structure, preventing the bag wall from collapsing and sticking to the body, and ensuring the stability of the internal operating space. 4) Intracavitary dissection and sampling procedures Sampling personnel put their hands into one-piece sealed operating gloves and complete the tissue cutting, sample loading into sampling tubes, and tube cap tightening operations in the sealed cavity; the volume of the tissue block obtained by cutting is controlled to be 0.2–0.5 cm³, which is adapted to the permeability characteristics of RNAlater protective solution at polar low temperature and the operation requirements of the operating gloves; different sterile dissection tools are used for different collection sites, and two copies of each sample are collected in parallel; 5) Disinfection and aseptic removal of sampling tubes The sealed sampling tube is sent from the main cavity into the extended disinfection channel, and the sampling tube is thoroughly soaked and disinfected using the disinfectant solution pre-filled in the extended disinfection channel. After disinfection, the sampling tube is taken out from the external outlet at the far end of the extended disinfection channel. The far end sealing structure is closed immediately after removal, and the cubic inflatable main cavity is kept sealed throughout the process. The outer wall of the removed sampling tube is disinfected a second time, and information is marked. Then it is placed in a portable freezer for temporary storage. 6) Contaminant sealing and encapsulation The corpses, tissue residues, blood, feathers and other contaminants inside the cavity are all placed into built-in independent sealed recycling bags and sealed, marked as infectious waste. All waste is taken out of the polar region for disposal and is not left on-site, buried or incinerated. 7) Chamber sterilization, exhaust and overall recovery The interior of the cube-shaped inflatable main chamber is thoroughly sterilized; the gas inside the chamber is safely discharged after being sterilized and disinfected only through a top-mounted virus-grade high-efficiency sterilization filter; after the chamber is vented, it naturally deflates, allowing the entire sampling device to be retrieved as a whole and subsequently disposed of in accordance with biosafety standards, making the device reusable.

[0016] Furthermore, during the sampling operation, the camera auxiliary component transmits images inside the cavity in real time to achieve visual assistance; when the tool touches the sensing area of ​​the operating glove, the anti-cutting early warning sensing component triggers an alarm.

[0017] Compared with the prior art, the beneficial effects of the present invention are: This invention addresses the unique challenges of polar environments characterized by low temperatures, strong winds, ecological sensitivity, and high biosafety risks. It designs a cubic inflatable in-situ closed sampling device and corresponding sampling method, overcoming the technical bottlenecks of traditional inflatable sampling bags, which suffer from lack of support, susceptibility to leakage, limited protection, and inconvenient operation. The entire solution achieves fully closed sampling, zero viral aerosol diffusion, and zero pollution of the polar ecosystem. Furthermore, intelligent auxiliary structures enhance operational safety and sampling accuracy. The device is cold-resistant, reusable, and balances personnel protection, sample quality, ecological conservation, and scientific research practicality. It is perfectly suited for in-situ sampling of highly pathogenic viruses from dead migratory birds and penguins in polar regions such as Antarctica. Attached Figure Description

[0018] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the sampling device of the present invention.

[0020] Figure 2 This is a flowchart illustrating the sampling method of the present invention.

[0021] The markings in the attached diagram are as follows: 1—cubic inflatable main chamber, 2—sterilizing filter, 3—filling window, 4—operating glove, 5—extended disinfection channel, 6—wide-angle miniature camera. Detailed Implementation

[0022] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only a part of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] like Figure 1As shown, an in-situ sealed sampling device for migratory bird-borne avian influenza virus in polar strong wind environments includes a cubic inflatable main cavity 1. The cubic inflatable main cavity 1 is made of a composite membrane that is resistant to low temperatures of -30℃, puncture-proof, highly airtight, and highly transparent. A one-way inflation valve for filling with clean air is provided on one side of the cubic inflatable main cavity. A portable hand-cranked clean air pump is used, and the pump body has a built-in 0.22μm pre-filter membrane to filter pollen, ice crystals, and exogenous microorganisms in the external polar air in advance, avoiding external contamination. After inflation, the cavity forms a rigid cubic operating space without wrinkles or collapse.

[0024] The top of the cube-shaped inflatable main cavity is equipped with a 0.22μm virus-grade sterilization filter 2. The air inside the cavity is sterilized by the sterilization filter 2 and then discharged to the outside.

[0025] The cube-shaped inflatable main cavity 1 has a zipper-type sealable filling window 3 on its side. The filling window 3 is used to pre-load dissection consumables before sampling. It can be repeatedly opened and completely sealed. The bottom of the cube-shaped inflatable main cavity 1 has a bottom window, which is used to insert the carcass of diseased or dead animals during sampling.

[0026] A pair of operating gloves 4 are integrally sealed in the center of the front of the cube-shaped inflatable main cavity. The inside of the operating gloves 4 is connected to the space of the cube-shaped inflatable main cavity. Operators can use the operating gloves 4 to complete dissection and sampling operations in the sealed cavity.

[0027] The sampling device also includes an extended disinfection channel 5; the outer side of the cube-shaped inflatable main cavity 1 is connected to a straight tubular extended disinfection channel 5, and the two ends of the extended disinfection channel 5 are respectively provided with independent sealing structures; the proximal end of the extended disinfection channel is sealed and connected to the cube-shaped inflatable main cavity, and the distal end is an external outlet, and the channel is pre-filled with disinfectant; the sampling tube can be sent into the extended disinfection channel from the main cavity for immersion disinfection, and taken out from the distal external outlet, keeping the cube-shaped inflatable main cavity sealed throughout the process.

[0028] The sampling device also includes a corpse sealing and recycling component; the cube-shaped inflatable main cavity is equipped with an independent sealing and recycling bag. After sampling, the corpse, tissue residue, feathers and other contaminants can be put into the sealing and recycling bag and sealed. The cube-shaped inflatable main cavity can be sterilized inside and the gas can be safely discharged through the top sterilization filter before collapsing. The whole can be repeatedly recycled.

[0029] The sampling device also includes a cut-resistant early warning sensor assembly for the operating glove. A low-temperature resistant flexible cut-sensor array is embedded within the interlayer of the operating glove, with sensor units distributed in areas easily cut by the blade, such as the fingers, palm, and web of the hand. All sensor circuitry is embedded within the membrane layer of the operating glove, without penetrating the cavity or the glove's sealing wall, ensuring the overall airtightness of the device. The sampling device is also equipped with a low-temperature audible, visual, and vibration alarm terminal, which is connected to the cut-sensor array. When the dissecting blade cuts or squeezes the sensing area, the alarm terminal triggers an audible, visual, and vibration warning, reminding the operator to adjust their actions to avoid damage to the operating glove.

[0030] The sampling device also includes a built-in camera auxiliary component; a low-temperature resistant wide-angle miniature camera 6 is embedded in the inner top of the cubic inflatable main cavity, next to the sterilization filter. The base of the wide-angle miniature camera 6 is heat-sealed with the cavity composite membrane, and the exit point of the wide-angle miniature camera is treated with multi-layer sealing. The lens of the wide-angle miniature camera is coated with an anti-fog coating. The device is also equipped with a wireless image transmission module, a low-temperature display terminal, and a video storage module. The wide-angle miniature camera captures the operation scene inside the cavity in real time and transmits it to the display terminal via wireless image transmission. The video storage module records the operation video simultaneously, realizing visual auxiliary operation and scientific research tracing.

[0031] like Figure 2 As shown, an in-situ sealed sampling device for avian influenza virus from migratory birds in polar strong wind environments is described. This sampling method, based on the aforementioned sampling device, specifically includes the following steps: 1) Pre-installation of consumables and window sealing Through the loading window on the side of the sampling device, sterile dissecting scissors, sterile forceps, sterile sampling tubes containing RNAlater protective solution, and disinfectant are loaded into the cubic inflatable main cavity. After the consumables are loaded, the loading window is completely sealed to put the device in a closed initial state.

[0032] 2) In-situ loading of the body and sealing of the entire compartment Upon discovering the carcass of a dead migratory bird or penguin, keep the carcass in its original position and posture to avoid disturbance that could cause aerosol and feather spread. The sampling personnel should stand upwind of the carcass, open the bottom window, and gently place the carcass into the cubic inflatable main cavity. Then, thoroughly seal the bottom window (not shown) to create a leak-free, fully enclosed structure.

[0033] 3) Inflatable molding constructs a rigid operating space Clean air is injected into the cavity through the one-way inflation valve on the cubic main inflatable cavity, causing the cavity to expand into a standard cubic rigid structure, preventing the bag wall from collapsing and sticking to the body, and ensuring the stability of the internal operating space.

[0034] 4) Intracavitary dissection and sampling procedures Sampling personnel, with both hands encased in integrated sealed operating gloves, perform tissue cutting, sample loading into sampling tubes, and tightening of tube caps within a sealed chamber. The volume of the cut tissue blocks is controlled to be 0.2–0.5 cm³, adapted to the permeability characteristics of the RNAlater protective solution at polar low temperatures and the operational requirements of the operating gloves. Separate sterile dissection tools are used for different sampling sites, and two samples are collected for each sample. During the procedure, a camera-assisted component transmits real-time images of the chamber to aid in precise sampling. If the blade touches the sensing area of ​​the operating glove, an anti-cutting warning component immediately sounds an alarm to avoid the risk of glove damage. After sampling, contaminants such as cadavers and tissue residues are temporarily stored within the chamber.

[0035] 5) Disinfection and aseptic removal of sampling tubes The sealed sampling tube is inserted from the main cavity into the extended disinfection channel, where it is thoroughly immersed in the pre-filled disinfectant solution. After disinfection, the sampling tube is removed from the external retrieval port at the far end of the extended disinfection channel. The distal sealing structure is immediately closed after removal, maintaining the cubic inflatable main cavity sealed throughout the process. The outer wall of the removed sampling tube is then disinfected a second time, labeled with information, and temporarily stored in a portable freezer.

[0036] 6) Contaminant sealing and encapsulation All the corpses, tissue residues, blood, feathers and other contaminants inside the cavity are placed into built-in independent sealed recycling bags and sealed, marked as infectious waste. All waste is taken out of the polar region for disposal and is not left on-site, buried or incinerated.

[0037] 7) Chamber sterilization, exhaust and overall recovery The interior of the cubic inflatable main chamber undergoes comprehensive sterilization. Gas within the chamber is safely discharged only after passing through a top-mounted, high-efficiency virus-grade sterilization filter for disinfection and sterilization. After the chamber is vented, it naturally deflates, allowing the entire sampling device to be retrieved and disposed of according to biosafety standards. The device can then be reused.

[0038] The process includes a comprehensive sterilization treatment of the cube-shaped inflatable main cavity, specifically involving spraying a low-temperature peracetic acid disinfectant evenly to cover all spaces inside the cube's inflatable main cavity, followed by a sealed and static sterilization period of 15 minutes (similar to this specific method of sterilizing the cavity). Sampling personnel wear biosafety level 3 personal protective equipment, including N95 or higher respirators, full face masks, waterproof and impermeable protective clothing, operating gloves and protective boot covers; at the same time, they wear sensor alarm terminals on their wrists and image display terminals fixed to the outside of their bodies.

[0039] First, through the loading window on the side of the sampling device, fill the cubic inflatable main chamber with sterile dissecting scissors, sterile dissecting forceps, sterile sampling tubes containing RNAlater protective solution, and disinfectant. After loading, seal the loading window on the side. Keep the dead migratory bird carcass in its original position and posture, and have the sampling personnel stand upwind. Open the bottom window to place the carcass in, and then completely seal the bottom window.

[0040] Clean air is injected into the chamber via a one-way inflation valve, causing it to expand into a rigid, standard cube-shaped space. The wide-angle miniature camera remains stable, providing clear imaging. Sampling personnel place their hands into integrated operating gloves to perform dissections. A flexible cutting sensor array within the gloves monitors external forces in real time. When the blade touches a dangerous area, an alarm terminal on the wrist immediately emits an audible, visual, and vibration alert. The wide-angle miniature camera at the top of the chamber transmits the internal images to an external display screen in real time. Based on this image, the operator precisely cuts tissue, strictly controlling the tissue block volume to 0.2–0.5 cm³. The equipment simultaneously records the operation video for scientific archiving. Independent sterilization instruments are used for different sampling sites, and two copies of each sample are collected.

[0041] After sampling, the sealed sampling tube is immersed in the extended disinfection channel for disinfection. After disinfection, the sampling tube is taken out from the far end of the channel and the far end sealing structure is closed. The outer wall of the sampling tube is disinfected and labeled with information, then placed in a sealed bag and temporarily stored in a portable freezer at low temperature.

[0042] The migratory bird carcasses, tissue residue, feathers, etc., were then placed into an independent, sealed recycling bag inside the chamber and sealed. The entire interior of the main chamber was sterilized, and the gas inside the chamber was safely discharged after being filtered through a virus-grade high-efficiency sterilization filter at the top. After the chamber collapsed, the entire device was retrieved and taken out of the polar region for harmless disposal. Samples were kept under cryogenic sealing throughout the entire process and stored in a special cabinet after being transported to the laboratory.

[0043] The above description is merely a preferred embodiment of the present invention. It should be understood that the preferred embodiments disclosed in this application are illustrative of the principles of the implementation scheme of the present invention and are not intended to limit the present invention. Any equivalent structural transformations made based on the inventive concept of the present invention and the description and drawings of the present invention, or direct / indirect applications in other related technical fields, are included within the scope of patent protection of the present invention.

Claims

1. A device for in-situ closed sampling of avian influenza virus from migratory birds in polar strong wind environments, characterized in that, The device includes a cubic inflatable main cavity; the cubic inflatable main cavity is made of a composite membrane that is resistant to low temperatures of -30℃, puncture-proof, highly airtight, and highly transparent. A one-way inflation valve is located on one side of the cavity, forming a rigid cubic operating space after inflation. A 0.22μm virus-grade high-efficiency sterilization filter is located at the top of the cubic inflatable main cavity. A zipper-type sealable filling window is located on the side of the cavity, and a bottom window is located on the bottom surface of the cavity. A pair of operating gloves are integrally sealed on the front of the cavity, and the inside of the operating gloves is connected to the cavity. It also includes an operating glove anti-cutting warning sensor assembly; a low-temperature resistant flexible cut-sensor array is arranged within the interlayer of the operating glove, and the sensing circuitry is embedded within the operating glove membrane layer and does not penetrate the sealing wall. The sampling device is equipped with a low-temperature audible and visual vibration alarm terminal, which is connected to the anti-cutting sensor array of the operating glove via a signal connection.

2. The sampling device according to claim 1, characterized in that, It also includes an extended disinfection channel; the extended disinfection channel is a straight tube with independent sealing structures at both ends. The proximal end of the extended disinfection channel is sealed and connected to the cubic inflatable main cavity, and the distal end is an external outlet. The channel is pre-filled with disinfectant.

3. The sampling device according to claim 2, characterized in that, The cube-shaped inflatable main cavity is equipped with an independent sealed recycling bag.

4. The sampling device according to claim 3, characterized in that, The cutting sensor array and the low-temperature audible and visual vibration alarm terminal are resistant to operating temperatures as low as -30℃.

5. The sampling device according to claim 4, characterized in that, It also includes a built-in camera auxiliary component; a low-temperature resistant wide-angle miniature camera is embedded in the inner top of the cubic inflatable main cavity, and the base of the wide-angle miniature camera is heat-sealed with the cavity membrane; the sampling device is equipped with a wireless image transmission module and a low-temperature display terminal.

6. The sampling device according to claim 5, characterized in that, The wide-angle miniature camera lens is equipped with an anti-fog coating, and the sampling device is also equipped with a video storage module; the wide-angle miniature camera, wireless image transmission module, low-temperature display terminal, and video storage module are all resistant to low-temperature environments of -30℃.

7. A method for in-situ sealed sampling of migratory bird-borne viruses under polar strong wind conditions based on the sampling device described in claim 6, characterized in that, Includes the following steps: 1) Pre-installation of consumables and window sealing Through the loading window on the side of the sampling device, sterile dissecting scissors, sterile forceps, sterile sampling tubes containing RNAlater protective solution, and disinfectant are loaded into the cubic inflatable main cavity. After the consumables are loaded, the loading window on the side is completely sealed to put the device in a closed initial state. 2) In-situ loading of the body and sealing of the entire compartment After discovering the carcass of a dead migratory bird or penguin, keep the carcass in its original position and posture to avoid disturbing it and causing the spread of aerosols and feathers. The sampling personnel should stand upwind of the carcass, open the bottom window, and place the carcass steadily into the cubic inflatable main cavity. Then, completely seal the bottom opening to make the entire cavity a leak-free, fully enclosed structure. 3) Inflatable molding constructs a rigid operating space Clean air is injected into the cavity through the one-way inflation valve on the cubic main inflatable cavity, causing the cavity to expand into a standard cubic rigid structure, preventing the bag wall from collapsing and sticking to the body, and ensuring the stability of the internal operating space. 4) Intracavitary dissection and sampling procedures Sampling personnel put their hands into one-piece sealed operating gloves and complete the operations of tissue cutting, sample loading into sampling tubes, and tightening tube caps in a closed cavity; the volume of the tissue blocks obtained by cutting is controlled to be 0.2–0.5 cm³, which is adapted to the permeability characteristics of RNAlater protective solution at polar low temperatures and the operation requirements of the operating gloves; different sterile dissection tools are used for different collection sites, and two copies of each sample are collected in parallel; 5) Disinfection and aseptic removal of sampling tubes The sealed sampling tube is sent from the main cavity into the extended disinfection channel, and the sampling tube is thoroughly soaked and disinfected using the disinfectant solution pre-filled in the extended disinfection channel. After disinfection, the sampling tube is taken out from the external outlet at the far end of the extended disinfection channel. The remote sealing structure is closed immediately after taking it out, and the cubic inflatable main cavity is kept sealed throughout the process. The outer wall of the taken-out sampling tube is disinfected a second time and labeled with information, and then placed in a portable freezer for temporary storage. 6) Contaminant sealing and encapsulation The corpses, tissue residues, blood, feathers and other contaminants inside the cavity are all placed into built-in independent sealed recycling bags and sealed, marked as infectious waste. All waste is taken out of the polar region for disposal and is not left on-site, buried or incinerated. 7) Chamber sterilization, exhaust and overall recovery The interior of the cube-shaped inflatable main cavity is thoroughly sterilized. The gas inside the cavity is safely discharged after being sterilized and disinfected only by passing through the top virus-grade high-efficiency sterilization filter; After the cavity is vented, it naturally collapses, allowing the entire sampling device to be retrieved. Subsequent harmless disposal is carried out in accordance with biosafety regulations, and the device can be reused.

8. The sampling method according to claim 7, characterized in that, During the sampling operation, the camera auxiliary component transmits images inside the cavity in real time to achieve visual assistance; when the tool touches the sensing area of ​​the operating glove, the anti-cutting early warning sensing component triggers an alarm.

Images