Biochemical reaction device

Abstract

The biochemical reaction device comprises a fluid storage module, a pipetting module, first and second biochemical reaction modules and a preparation and mixing module. The fluid storage module comprises a first storage assembly and a second storage assembly. The first and second biochemical reaction modules are in communication with the pipetting module. The preparation and mixing module is integrated in the fluid storage module, and can be detachably connected with the first storage assembly and used for mixing the fluid in the first storage assembly. The pipetting module can transfer the fluid in the first storage assembly to the first biochemical reaction module to perform a first biochemical reaction and obtain a reaction product. The pipetting module can also transfer the reaction product and the fluid in the second storage assembly to the second biochemical reaction module to perform a second biochemical reaction. The biochemical reaction device can realize multiple steps such as fluid mixing, sample preparation and analysis and detection, and has high integration and automation.

Description

Technical Field

[0001] This application relates to the field of biochemistry, and more particularly to a biochemical reaction apparatus. Background Technology

[0002] With the development of gene sequencing technology, gene sequencers are evolving towards high throughput, low cost, miniaturization, automation, and simplified operation. Currently, a complete gene sequencing process typically includes several steps: library construction, sample preparation, sample loading, and sequencing. Each of these steps requires various biological samples (including human blood samples, tissue samples, or saliva samples), adapters, and various reagents required for biochemical reactions.

[0003] Currently, the liquids involved in each of the above steps are usually stored and used independently, which takes up a lot of storage space, is inconvenient to handle, and is difficult to meet the current requirements for high integration, high automation and miniaturization of gene sequencing equipment. Summary of the Invention

[0004] In view of this, it is necessary to propose a biochemical reaction device in order to solve at least one of the above defects.

[0005] This application provides a biochemical reaction apparatus, including: a fluid storage module, a pipetting module, a first biochemical reaction module, a second biochemical reaction module, and a preparation and mixing module. The fluid storage module includes a first storage unit, which includes a first storage box and a first storage component and a second storage component detachably disposed within the first storage box. Both the first and second storage components are used to store fluids required for the biochemical reaction. The pipetting module is located above the fluid storage module. The first biochemical reaction module is connected to the pipetting module and is used to perform a first biochemical reaction. The second biochemical reaction module is connected to both the pipetting module and the first biochemical reaction module and is used to perform a second biochemical reaction. The preparation and mixing module is integrated into the fluid storage module and is detachably connected to the first storage component. The preparation and mixing module is used to mix the fluid in the first storage component. The pipetting module is used to transfer fluid from the first storage component to the first biochemical reaction module to perform the first biochemical reaction and obtain reaction products; the pipetting module is also used to transfer the reaction products and fluid from the second storage component to the second biochemical reaction module to perform the second biochemical reaction.

[0006] In some possible embodiments, the first storage box includes a first storage compartment, the first storage component includes a first storage element detachably disposed in the first storage compartment, and the fluid storage module further includes a temperature control structure located in the first storage compartment and used to control the temperature of the fluid within the first storage element.

[0007] In some possible embodiments, the temperature control structure includes: a temperature conduction component, an auxiliary temperature control component, and a temperature control component. The temperature conduction component is disposed on the auxiliary temperature control component, and the temperature control component is located between the temperature conduction component and the auxiliary temperature control component. The temperature conduction component can contact the first storage device.

[0008] In some possible embodiments, the first storage position is located at one end of the first storage box, the auxiliary temperature control component is located outside the first storage box, the temperature conduction component is located below the first storage position, and the temperature conduction component has a temperature control hole, and the first storage component is detachably disposed at the temperature control hole.

[0009] In some possible embodiments, the first storage box further includes a second storage position with a clearance space below it, the preparation and mixing module is disposed in the clearance space, and the first storage component further includes a second storage element detachably disposed in the second storage position, the second storage element being detachably connected to the preparation and mixing module.

[0010] In some possible embodiments, the second storage component includes a limiting part and a plurality of mixing containers detachably disposed within the limiting part. The limiting part is detachably connected to the first storage position. Each mixing container includes a hollow container body, a locking part located at the bottom of the container body, and a protrusion disposed on the inner wall of the container body. The locking part can lock onto the preparation mixing module.

[0011] In some possible embodiments, the first storage box further includes a third storage compartment, and the first storage component further includes a third storage element detachably disposed in the third storage compartment, wherein the openings of the second storage element are arranged side by side with the openings of the third storage element.

[0012] In some possible embodiments, the first storage box further includes a cleaning tank and two cleaning holes disposed in the cleaning tank. The two cleaning holes are respectively located at the same end of the second storage position and the third storage position. The cleaning holes are used to clean the liquid picking needle of the pipetting module, and the cleaning tank is used to contain cleaning waste liquid.

[0013] In some possible embodiments, the storage structure includes a mounting frame and a storage frame slidably disposed on the mounting frame, the first storage unit being located within a first storage cavity formed by the storage frame; the storage structure further includes a locking member and a retaining member, the locking member being used to lock or unlock the storage frame, and the retaining member being used to provide auxiliary holding force to the storage frame when the storage frame is locked, and to provide feedback information that the storage frame is locked in place.

[0014] In some possible embodiments, the storage structure further includes a second storage cavity, and the fluid storage module further includes a second storage unit detachably disposed in the second storage cavity, the second storage unit being used to store cleaning fluid and waste fluid.

[0015] The biochemical reaction apparatus provided in this application integrates multiple storage components into a first storage unit, concentrating various fluids in the same storage box. This fluid storage module can be integrated into the biochemical reaction apparatus and, in conjunction with a pipetting module, achieves centralized scheduling and transfer of fluids, improving the efficiency and convenience of fluid transfer. Furthermore, the fluid storage module has a reasonable layout and high space utilization. Moreover, the fluid storage module, combined with a preparation and mixing module, enables fluid mixing operations. In addition, in conjunction with the first and second biochemical reaction modules, it enables sample preparation and analysis detection steps, improving the integration and automation level of the biochemical reaction apparatus.

[0016] Furthermore, by incorporating a temperature control structure within the fluid storage module, localized temperature control can be achieved. Only individual temperature-sensitive reagents need to be controlled, eliminating the need for temperature control of the entire fluid storage module. The temperature control structure is compact, occupies little space, and can be directly integrated into the fluid storage module. This temperature control structure also has low energy consumption, reducing the power supply requirements and overall power consumption, and effectively reducing the amount of condensate, thus minimizing mold growth caused by condensate. Attached Figure Description

[0017] Figure 1 A modular architecture diagram of a biochemical reaction apparatus provided in one embodiment of this application.

[0018] Figure 2 This is a schematic diagram of the structure of a fluid storage module provided in one embodiment of this application.

[0019] Figure 3 for Figure 2 A schematic diagram of the structure in which the storage frame is pulled out by the mounting bracket.

[0020] Figure 4 for Figure 3 An exploded view of part of the structure of the fluid storage module.

[0021] Figure 5for Figure 3 A schematic diagram of the structure of the storage frame.

[0022] Figure 6 for Figure 5 A structural diagram of the storage frame from another perspective.

[0023] Figure 7 for Figure 3 A schematic diagram of the structure in the medium fluid storage module where the storage frame and mounting bracket are not engaged.

[0024] Figure 8 for Figure 4 A schematic diagram of the structure of the first storage unit.

[0025] Figure 9 for Figure 8 A schematic diagram of the structure after the first storage unit is combined with the temperature control structure and the preparation and mixing module.

[0026] Figure 10 for Figure 9 Cross-sectional view along XX.

[0027] Figure 11 for Figure 9 Exploded view of the structure shown.

[0028] Figure 12 for Figure 11 A schematic diagram of the structure of the second storage device.

[0029] Figure 13 for Figure 12 The exploded view of the second storage device is shown.

[0030] Figure 14 for Figure 11 Exploded view of the third storage device.

[0031] Figure 15 for Figure 11 Exploded view of the fourth storage device.

[0032] Figure 16 for Figure 4 A schematic diagram of the central temperature control structure.

[0033] Figure 17 for Figure 16 The exploded view of the temperature control structure shown.

[0034] The following detailed description, in conjunction with the accompanying drawings, will further illustrate this application. Detailed Implementation

[0035] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments.

[0036] It should be noted that when a component is described as "fixed to" or "mounted to" another component, it can be directly on the other component or may be interspersed with an intermediate component. When a component is described as "set to" another component, it can be directly set on the other component or may be interspersed with an intermediate component. The term "and / or" as used herein includes all and any combination of one or more of the associated listed items.

[0037] It should be noted that although a logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in a different order than that shown in the flowchart. The methods disclosed in the embodiments of this application include one or more steps or actions for implementing the method. Method steps and / or actions may be interchanged with each other without departing from the scope of the claims. Unless a specific order of steps or actions is specified, the order and / or use of specific steps and / or actions may be modified without departing from the scope of the claims.

[0038] Please see Figure 1 As shown, one embodiment of this application provides a biochemical reaction apparatus 100. The biochemical reaction apparatus 100 includes a fluid storage module 10 and a pipetting module 20. The fluid storage module 10 stores various fluids required for the biochemical reaction, and the pipetting module 20 transfers the fluids within the fluid storage module 10. The fluids may be, for example, liquids such as biological samples (biological samples may be nucleic acid samples, human blood samples, tissue samples, or saliva samples, etc.), adapters, and reagents used in biochemical analysis; they may also be sample carriers (e.g., magnetic beads) used to carry biological samples in sequencing library construction; or they may be mixed liquids of biological samples, reagents, sample carriers, etc., but are not limited to these. For example, the biochemical reaction apparatus 100 may be a gene sequencing device, and the fluid storage module 10 may store the biochemical substances and reagents required for sample preparation before sequencing and the sequencing reaction.

[0039] The biochemical reaction apparatus 100 may further include a first biochemical reaction module 30 and a second biochemical reaction module 40. The pipetting module 20 can transfer fluid from the fluid storage module 10 to the first biochemical reaction module 30, which can perform a first biochemical reaction; for example, the first biochemical reaction module 30 may be a PCR instrument capable of nucleic acid amplification. The pipetting module 20 can also transfer fluid from the fluid storage module 10 and the reaction products from the first biochemical reaction module 30 to the second biochemical reaction module 40, which can perform a second biochemical reaction; for example, the second biochemical reaction module 40 may be a sequencing module capable of gene sequencing.

[0040] Understandably, the biochemical reaction device 100 may also include a drive module 60, which can be connected to the pipetting module 20 to provide driving force for fluid transfer and adjust the direction of fluid transfer. It is also understood that the biochemical reaction device 100 may include a control module 50, which can be used to control the coordinated operation between the fluid storage module 10, the pipetting module 20, the first biochemical reaction module 30, the second biochemical reaction module 40, and the drive module 60.

[0041] Please see Figures 2 to 4 As shown, in conjunction with participation Figure 1 As shown, the fluid storage module 10 includes a storage structure 1 and a first storage unit 2. The storage structure 1 includes a first storage cavity 11, and the first storage unit 2 is detachably disposed within the first storage cavity 11. The first storage unit 2 includes a first storage box 4 and multiple storage components 5. The first storage box 4 includes multiple storage positions 41, and each storage position 41 is detachably provided with at least one storage component 5. The storage component 5 is used to store fluids required for biochemical reactions. Specifically, the storage component 5 can be used to store biological samples and various reagents required for biochemical reactions. The multiple storage components 5 can be divided into a first storage assembly and a second storage assembly, used to store two types of fluids. The fluid in the first storage assembly is mainly used in the first biochemical reaction module 30 to achieve sample preparation, such as the preparation of amplification products; while the fluid in the second storage assembly is mainly used in the second biochemical reaction module 40 to achieve biochemical analysis and detection, such as gene sequencing.

[0042] In some embodiments, the fluid storage module 10 may further include a second storage unit 6, and the storage structure 1 may further include a second storage cavity 12, wherein the second storage unit 6 is detachably disposed within the second storage cavity 12. Specifically, the second storage unit 6 includes a second storage box 61, which can be used to store cleaning solution and waste liquid. For example, for a gene sequencing process, the first storage unit 2 may be a sequencing kit for storing samples and reagents, and the second storage unit 6 may be a cleaning solution kit.

[0043] Please see Figure 5 and Figure 6 As shown, the storage structure 1 also includes a mounting bracket 13 and a storage frame 14 disposed within the mounting bracket 13. The storage frame 14 can be a hollow frame structure, forming a first storage cavity 11 and a second storage cavity 12. Specifically, the storage frame 14 can slide relative to the mounting bracket 13 to push into or pull out the mounting bracket 13, facilitating the retrieval of the first storage unit 2 and the second storage unit 6. For example, the storage frame 14 can be a drawer. In some embodiments, the storage structure 1 also includes a slider 19, which enables the storage frame 14 to slide relative to the mounting bracket 13. Specifically, the slider 19 can be a slide rail, such as a ball bearing slide rail. In some embodiments, the front side panel of the storage frame 14 is also provided with a handle 18, facilitating the user to pull out the storage frame 14.

[0044] like Figure 6 As shown, the storage structure 1 also includes a locking element 15, which is used to lock or unlock the storage frame 14. When the storage frame 14 is pushed into the mounting bracket 13, the locking element 15 can lock the storage frame 14 onto the mounting bracket 13. When the storage frame 14 is pulled, the locking element 15 unlocks, allowing the storage frame 14 to be pulled out from the mounting bracket 13. Specifically, the locking element 15 may include a first locking part 151 located at the bottom of the storage frame 14 and a second locking part 152 located at the bottom of the mounting bracket 13. The first locking part 151 can engage with the second locking part 152 to lock the storage frame 14 within the mounting bracket 13, preventing the storage frame 14 from being accidentally pulled out during use. In some embodiments, the locking element 15 may be an electronic lock.

[0045] like Figure 6 and Figure 7 As shown, the storage structure 1 also includes a retaining member 16. The retaining member 16 provides auxiliary holding force for the storage frame 14 after it is locked in the mounting bracket 13, and feeds back the information that the storage frame 14 is locked in place to the control module 50 to facilitate the initiation of the next process. In some embodiments, the retaining member 16 may include a first retaining part 161 provided at the bottom of the storage frame 14 and a second retaining part 162 provided at the bottom of the mounting bracket 13. The first retaining part 161 includes a roller 163, and the second retaining part 162 includes a locking block 164. When the storage frame 14 is pushed into the mounting bracket 13, the first retaining part 161 will drive the roller 163 to roll and lock onto the locking block 164 of the second retaining part 162, thereby providing auxiliary holding force for locking the storage frame 14. In addition, the first retaining part 161 may also include a sensing component (not shown), which can transmit a corresponding sensing signal to the control module 50 when the roller 163 is engaged or disengaged from the locking block 164. Specifically, the first holding part 161 can be a roller-type ball buckle, and the second holding part 162 can be a ball buckle base.

[0046] like Figure 6As shown, the storage structure 1 may further include two position sensors 17, located in the first storage cavity 11 and the second storage cavity 12, respectively. The two position sensors 17 are used to determine whether the first storage unit 2 and the second storage unit 6 are placed in the storage frame 14.

[0047] Please see Figures 8 to 11 As shown, the multiple storage positions 41 in the first storage box 4 may include first storage positions 411, which are located at one end of the first storage box 4. The multiple storage components 5 may include a first storage component 51 detachably disposed in the first storage position 411; that is, the first storage assembly may include the first storage component 51. In some embodiments, the first storage position 411 may include a storage hole, and the first storage component 51 may be installed in the storage hole. In some embodiments, the first storage component 51 may include a first storage cavity 511 and a first hole 512, which can store a fluid; for example, the first storage component 51 may be a cryovial. In other embodiments, the first storage component may also have multiple separately arranged first storage cavities 511, each first storage cavity 511 having a first hole 512, capable of storing multiple fluids. Specifically, the first storage component 51 may contain reagents required for sample preparation in the pre-sequencing steps of gene sequencing.

[0048] In some embodiments, the first storage unit 51 is further provided with a first identification code 513 for identifying information about the fluid inside the first storage unit 51.

[0049] Please refer to it again. Figures 8 to 11 As shown, the multiple storage locations 41 also include a second storage location 412, and the multiple storage components 5 also include a second storage component 52. That is, the first storage component may also include a second storage component 52, which is detachably disposed in the second storage location 412. For example, in the pre-sequencing steps of gene sequencing, the second storage component 52 can be used to store the biological samples and reagents required for sample preparation.

[0050] In some embodiments, in conjunction with reference Figure 12 and Figure 13The second storage unit 52 may include a limiting part 521 and at least one mixing container 522 located within the limiting part 521. For example, the limiting part 521 may have multiple spaced mixing containers 522 that can accommodate various fluids. The limiting part 521 can be limited within the second storage position 412, at which point the mixing container 522 is limited along the depth direction Z of the second storage position 412, while the mixing container 522 can also rotate freely within the limiting part 521. In some embodiments, the biochemical reaction apparatus 100 may further include a preparation mixing module 70. A clearance position 42 is provided below the second storage position 412, which can be used to install the preparation mixing module 70. After the second storage unit 52 is installed in the second storage position 412, it can be detachably connected to the preparation mixing module 70. The preparation mixing module 70 can drive the mixing container 522 in the second storage unit 52 to rotate around the axial direction (i.e., the depth direction Z) of the preparation mixing module 70, thereby achieving mixing of the fluids in the mixing container 522.

[0051] It is understandable that the mixing module 70 can be equipped with a drive mechanism capable of driving multiple mixing containers 522 in a coordinated manner. Specifically, such as... Figure 9 and Figure 13 As shown, the mixing module 70 may include a drive mechanism 701, a transmission assembly 702, and multiple rotating structures 703. The rotating structures 703 are mounted on the output shaft of the transmission assembly 702, and each rotating assembly 703 is detachably equipped with a mixing container 522. The drive mechanism 701 drives the transmission assembly 702 to rotate, thereby causing the rotating structures 703 and the mixing containers 522 to rotate, thus achieving the mixing of different fluids.

[0052] Please see Figure 12 and Figure 13 As shown, the limiting part 521 includes a base 523 and a cover plate 524. The base 523 includes at least one insertion hole 525, within which a mixing container 522 is detachably disposed. The cover plate 524 is detachably disposed at the end of the base 523 away from the clearance position 42, and the cover plate 524 has a through hole 526 corresponding to the insertion hole 525, the opening of the mixing container 522 communicating with the through hole 526. The mixing container 522 is axially confined within the cavity formed by the base 523 and the cover plate 524, and the mixing container 522 can rotate freely about its axial direction within this cavity.

[0053] In some embodiments, the mixing container 522 includes a hollow container body 527, a locking portion 528 located at the bottom of the container body 527, and a protrusion 529 provided on the inner wall of the container body 527. The locking portion 528 can engage with the rotating assembly 703, realizing a detachable connection between the mixing container 522 and the rotating assembly 703. Figure 8As shown. The protrusion 529 extends along the depth direction of the container body 527, or it can extend spirally on the inner wall. The protrusion 529 can improve the mixing effect. During rotation and stopping, the protrusion 529 on the inner wall of the container body 527 can generate vortex stirring of the fluid inside the container body 527, realizing the mixing of fluids with different components. In some embodiments, the number of protrusions 529 can be one or multiple, to further improve the mixing effect. In addition, the height of the protrusion 529 should not be too high, so as not to affect the pipetting module 20 from extending into the mixing container 522 for pipetting. In some embodiments, the mixing container 522 can have a sealing cap 5291. When in use, the sealing cap 5291 can be opened to realize fluid transfer. Before use, the sealing cap 5291 can protect the mixing container 522 from foreign objects entering.

[0054] In some embodiments, the second storage device 52 includes a plurality of second holes 520 arranged side-by-side along a first direction X, wherein the first direction X is perpendicular to the depth direction Z. That is, the second storage device 52 includes a plurality of mixing containers 522 arranged side-by-side along the first direction X, each mixing container 522 having an opening that constitutes a second hole 520 of the second storage device 52. Specifically, the second storage position 412 may be an elongated strip structure extending along the first direction X, the second storage device 52 is a cuboid structure, and the openings of the plurality of mixing containers 522 are located on the same straight line. In some embodiments, the second storage position 412 may be located at the edge of the first storage box 4 to facilitate the preparation of the mixing module 70 to fit with the second storage device 52 on the second storage position 412. In addition, it can make more reasonable use of the space of the first storage box 4 and the storage structure 1 (e.g., Figure 4 (As shown).

[0055] In some embodiments, the second storage unit 52 is further provided with a second identification code 5292 for identifying information about the fluid inside the second storage unit 52.

[0056] Please see Figure 8 , Figure 11 and Figure 14 As shown, the plurality of storage sites 41 also include a third storage site 413, and the plurality of storage components 5 also include a third storage component 53 detachably disposed at the third storage site 413, that is, the first storage component may include the third storage component 53. Along the first direction X, the third storage component 53 may include a plurality of third apertures 531 arranged side by side, that is, the third storage component 53 has a plurality of second storage cavities 532 separated from each other, each second storage cavity 532 having a third aperture 531, which can accommodate a variety of fluids, such as a variety of reagents required for sample preparation in the pre-sequencing steps of gene sequencing.

[0057] In some embodiments, a row of second holes 520 in the second storage unit 52 and a row of third holes 531 in the third storage unit 53 are arranged parallel to each other, that is, the arrangement direction of the plurality of second holes 520 and the arrangement direction of the plurality of third holes 531 are consistent. This design, on the one hand, can reasonably arrange the first storage unit 2, make full use of the space of the first storage box 4, and improve space utilization; on the other hand, since the fluids stored in the second storage unit 52 and the third storage unit 53 are both used for sample preparation, the two slitting needles on the pipetting module 20 can simultaneously perform slitting operations in the second holes 520 and the third holes 531 during the movement along the first direction X, which can effectively simplify the structural complexity of the pipetting module 20 and improve pipetting efficiency.

[0058] In some embodiments, the first storage position 411 is located at one end of the third storage position 413, thereby placing the first well position 512 and the third well position 531 in the same row. This allows the pipetting module 20 to further aspirate the fluid from the first well position 512 using the squeezing needle of the third well position 531, further improving the space utilization of the first storage unit 2 and simplifying the structural complexity of the pipetting module 20. Specifically, the pipetting module 20 can transfer the fluids in the first storage unit 51, the second storage unit 52, and the third storage unit 53 to the first biochemical reaction module 30 for biochemical reactions, such as the sample preparation process in the pre-sequencing steps of gene sequencing (see reference). Figure 1 (As shown).

[0059] In some embodiments, the third storage device 53 is further provided with a third identification code 533 for identifying information about the fluid inside the third storage device 53.

[0060] Please see Figure 8 , Figure 11 and Figure 15 As shown, the plurality of storage sites 41 further includes a fourth storage site 414, and the plurality of storage components 5 further includes a fourth storage component 54 detachably disposed at the fourth storage site 414, that is, the second storage component may include the fourth storage component 54. The fourth storage site 414 may be located around the first storage site 411. In some embodiments, the fourth storage component 54 may include a plurality of fourth storage cavities 541, each fourth storage cavity 541 having a fourth pore 542, which can accommodate various fluids, such as sequencing reagents required in gene sequencing processes.

[0061] In some embodiments, one of the plurality of fourth storage cavities 541 may be an empty fourth storage cavity 541 for stepwise mixing of the target fluid. For example, fluid from other fourth storage cavities 541 or fluid from other storage devices 5 may be quantitatively transferred to the empty fourth storage cavity 541 for mixing, according to the requirements of the actual reaction steps, and the mixed fluid is then transferred to the second biochemical reaction module 40 for reaction (see reference). Figure 1 (As shown).

[0062] In some embodiments, the fourth storage unit 54 is further provided with a fourth identification code 543 for identifying information about the fluid inside the fourth storage unit 54.

[0063] Please refer to it again. Figures 8 to 11 As shown, the plurality of storage locations 41 also include a fifth storage location 415, and the plurality of storage components 5 also include a fifth storage component 55 removably disposed within the fifth storage location 415, that is, the second storage component may also include a fifth storage component 55. The fifth storage component 55 can be used to store various fluids, such as primers required for gene sequencing.

[0064] The plurality of storage locations 41 also include at least one sixth storage location 416, and the plurality of storage components 5 also include a sixth storage component 56 removably disposed within the sixth storage location 416, that is, the second storage component may also include a sixth storage component 56. There may be multiple sixth storage components 56, each used for a fluid, such as various reagents required in gene sequencing. These sixth storage components 56 have a large volume and can store a large quantity of reagents.

[0065] In some embodiments, the fifth storage unit 55 and the sixth storage unit 56 are also provided with identification codes to identify information about the fluid inside the fifth storage unit 55 and the sixth storage unit 56.

[0066] Please refer to it again. Figures 8 to 11 As shown, please refer to the following: Figure 1 As shown, the first storage box 4 also includes a cleaning tank 43 and two cleaning holes 44 disposed within the cleaning tank 43. The two cleaning holes 44 are located at one end of the second storage position 412 and the third storage position 413, respectively, and are respectively set corresponding to the second hole position 520 and the third hole position 531. The cleaning holes 44 are used to clean the liquid-taking needles of the pipetting module 20, and the cleaning tank 43 is used to contain cleaning waste liquid. Specifically, the two liquid-taking needles in the pipetting module 20 used to transfer fluids in the first storage component 51, the second storage component 52, and the third storage component 53 can extend into the bottom of the corresponding cleaning holes 44. The cleaning liquid is ejected from the liquid-taking needles to fill the entire cleaning hole 44, and the ejection process is maintained for a period of time to achieve the flushing and cleaning of the outer wall of the liquid-taking needles. The cleaning liquid overflowing from the cleaning holes 44 flows into the cleaning tank 43 for temporary storage, thereby achieving backflushing and cleaning of the liquid-taking needles and temporarily collecting the backflushing waste liquid. Before the biochemical reaction is completed, the waste liquid in the cleaning tank 43 can be further pumped to the waste liquid recycling point.

[0067] Please see Figure 8As shown, the first storage box 4 also includes multiple positioning holes 45, which can be used to position the pipetting module 20, improving the alignment accuracy between the pipetting needle and the corresponding hole during the pipetting process. Specifically, the multiple positioning holes 45 may include oblong holes, circular holes, etc.

[0068] Please see Figure 16 and Figure 17 Please refer to both together. Figure 4 , Figure 5 and Figure 10 As shown, the fluid storage module 10 also includes a temperature control structure 3, which is used to control the temperature of the fluid within at least one storage unit 5. Specifically, the temperature control structure 3 can be disposed on the storage structure 1 and located at one end of the first storage cavity 11. In some embodiments, the temperature control structure 3 can be located below the first storage position 411 and in contact with the first storage unit 51, so that the temperature control structure 3 can achieve temperature control of the fluid within the first storage unit 51. In addition, depending on the aforementioned structure of the first storage unit 51, the temperature control structure 3 can control the temperature of the first storage unit having one storage cavity, or it can simultaneously control the temperature of the first storage unit having multiple storage cavities.

[0069] The temperature control structure 3 includes a temperature conduction component 31 located below the first storage position 411. The temperature conduction component 31 can contact the first storage element 51. In this way, the temperature control structure 3 can conduct heat down to the first storage element 51 or conduct heat out of the first storage element 51 through the temperature conduction component 31, thereby achieving temperature control of the fluid inside the first storage element 51. In some embodiments, the temperature control structure 3 can be disposed on the storage frame 14 of the storage structure 1 and located at one end of the first storage cavity 11. Specifically, the temperature control structure 3 is installed at the end of the first storage cavity 11 away from the handle position 18, which facilitates the installation and circuit layout of the temperature control structure 3, and also facilitates cooperation with the first storage position 411.

[0070] The temperature control structure 3 also includes an auxiliary temperature control component 32 and a temperature control component 33. A temperature conduction component 31 is disposed on the auxiliary temperature control component 32, and the temperature control component 33 is located between the temperature conduction component 31 and the auxiliary temperature control component 32. The temperature control component 33 is used to regulate the temperature of the temperature conduction component 31 so that the fluid in the storage unit 5 (specifically the first storage unit 51) reaches a preset temperature. The auxiliary temperature control component 32 is configured to assist the temperature control component 33 in regulating the temperature of the temperature conduction component 31, thereby assisting in temperature control of the first storage unit 51.

[0071] like Figure 10 , Figure 16 and Figure 17As shown, the temperature conduction component 31 includes a mounting base 311 and a conduction section 312. The mounting base 311 is connected to the auxiliary temperature control component 32, at which point the temperature control component 33 is located between the mounting base 311 and the auxiliary temperature control component 32. The conduction section 312 is located on the side of the mounting base 311 away from the auxiliary temperature control component 32. The conduction section 312 has a temperature control hole 313, which can accommodate a first storage component 51. The first storage component 51 is in contact with the inner wall of the temperature control hole 313, thereby enabling temperature control of the first storage component 51. For example, in a biochemical reaction process, if a portion of the fluid needs to be stored at low temperature, a first storage component 51 can be placed in the temperature control hole 313 for freezing. This first storage component 51 has the structure described above and can have one or more first holes 512, enabling the freezing of one or more fluids.

[0072] In some embodiments, the size of the temperature control orifice 313 can be designed according to the size of the container requiring temperature control. For example, in gene sequencing processes, typically only a few reagents are temperature-sensitive, while most other reagents can be used at room temperature. Therefore, only these reagents need to be refrigerated. In this case, the size of the temperature control orifice 313 can be small, enough to accommodate a small first storage device 51. Before use or during transportation, the first storage device 51 can be stored in a refrigerator and placed in the temperature control orifice 313 for low-temperature refrigeration during use to prevent reagent deterioration during prolonged sequencing. It is understood that multiple temperature control orifices 313 can be provided to insulate multiple reagents, or the size of the temperature control orifice 313 can be designed to be larger, allowing the first storage device 51 placed inside to have multiple independent pores and store various temperature-sensitive fluids. It is also understood that the temperature control structure 3 can be used to control the temperature of other storage devices 5 besides the first storage device 51.

[0073] like Figure 16 and Figure 17As shown, the auxiliary temperature control component 32 can assist in removing heat from the temperature control component 33, thereby achieving heat dissipation and reducing the temperature of the temperature control component 33. It can also assist in heating the temperature control component 33. The auxiliary temperature control component 32 may include a mounting plate 321, which has a mounting groove 322. On the side of the mounting plate 321 away from the mounting groove 322, there are multiple generally parallel teeth 323, and a channel 324 is formed between two adjacent teeth 323. The temperature conduction component 31 is connected to the mounting plate 321 and clamps the temperature control component 33 in the mounting groove 322. By providing multiple teeth 323, the temperature conduction area of ​​the auxiliary temperature control component 32 can be increased, the temperature conduction efficiency can be improved, and thus the cooling or heating rate of the temperature control component 33 can be increased. In some embodiments, the auxiliary temperature control component 32 can be a heat sink to assist in the heat dissipation of the temperature control component 33. Understandably, in other embodiments, the auxiliary temperature control component can also be a liquid circuit module, which can achieve the purpose of auxiliary cooling or auxiliary heating by introducing liquid with a certain temperature into the liquid circuit module.

[0074] In some embodiments, the auxiliary temperature control component 32 further includes a cooling fan 325. A channel 324 is formed between two adjacent teeth 323. The convection within the channel 324 can be increased by adding a cooling fan 325 or other devices to further improve the efficiency of temperature conduction.

[0075] In some embodiments, the cooling fan 325 is fixed to the outermost side of the channel 324 via the mounting duct 326, which allows each channel 324 to be connected to the cooling fan 325 and form a total cooling duct. The mounting duct 326 can be designed according to the location and installation space of the auxiliary temperature control component 32, thereby improving the installation flexibility of the temperature control structure 3.

[0076] like Figure 16 and Figure 17 As shown, the temperature control component 33 can be a thermoelectric cooler (or a thermoelectric cooler, TEC). TECs are characterized by being noiseless, vibration-free, requiring no refrigerant, small in size, and lightweight. They are also reliable, easy to operate, provide rapid cooling and heating, and offer highly precise temperature control. The temperature control component 33 includes a first surface 331 and a second surface 332 arranged opposite to each other. The first surface 331 is located closer to the temperature conduction component 31, and the second surface 332 is located closer to the auxiliary temperature control component 32. During heating, the first surface 331 is the hot surface, and the second surface 332 is the cold surface; during cooling, the first surface 331 is the cold surface, and the second surface 332 is the hot surface.

[0077] In some embodiments, the first surface 331 and the second surface 332 of the temperature control component 33 are both planar, which can clamp the temperature control component 33 between the temperature conduction component 31 and the auxiliary temperature control component 32 to improve the heat conduction efficiency.

[0078] In some embodiments, a thermally conductive material may be disposed between the temperature control component 33 and the auxiliary temperature control component 32, which is beneficial for heat transfer and precise temperature control. This thermally conductive material can be a thermally conductive pad, thermally conductive patch, or similar thermally conductive layer. It is understood that the thermally conductive material can also be a thermally conductive coating applied to the corresponding surfaces of the temperature control component 33 and the auxiliary temperature control component 32, such as silver grease, thermally conductive silicone grease, etc.

[0079] like Figure 10 , Figure 16 and Figure 17 As shown, the temperature control structure 3 also includes a detection component 34 for detecting whether the first storage component 51 is installed in the temperature control hole 313. The detection component 34 may be, for example, a positioning sensor.

[0080] The temperature control structure 3 is integrated on the storage frame 14, wherein the temperature conduction component 31 is located inside the first storage cavity 11, while the auxiliary temperature control component 32 is located outside the first storage cavity 11 to improve the heat dissipation effect.

[0081] In biochemical reactions (such as gene sequencing), due to the long reaction time, some temperature-sensitive components require refrigeration. Traditionally, a sealed refrigeration environment (e.g., a 4°C sealed refrigerator) is embedded within the biochemical reaction apparatus, and the entire fluid storage module is placed in this environment for temperature control. This type of sealed refrigerator is large, consumes a lot of power, and is prone to producing large amounts of condensate, leading to mold growth and potentially negatively impacting the biochemical reaction. In this application, a temperature control structure 3 is provided at one end of the first storage cavity 11. When the first storage box 4 is placed in the first storage cavity 11, the temperature control structure 3 is located at one end of the first storage box 4 (e.g., below the first storage position 411). Thus, when a storage component 5 (e.g., the first storage component 51) is placed in the corresponding storage position 41, it can contact the temperature conduction component 31, thereby achieving temperature control for certain storage components 5. This temperature control method enables localized temperature control, requiring only temperature control for individual temperature-sensitive reagents, without needing to control the temperature of the entire fluid storage module 10. The temperature control structure 3 is compact, occupies little space, and can be directly integrated into the fluid storage module 10. Moreover, this small temperature control structure 3 has low power consumption, effectively reducing the power supply requirements and thus reducing the power consumption of the biochemical reaction device 100. In addition, the temperature control range of the temperature control structure 3 can be set with minimal difference from room temperature, for example, it can be set to 16℃~20℃, which can effectively reduce the amount of condensate and reduce the mold problem caused by condensate.

[0082] Please see Figure 1 As shown, the biochemical reaction apparatus 100 may further include an identification module 80. Both the first storage unit 2 and the second storage unit 6 are provided with storage box identification codes 7, and the identification module 80 is used to identify the storage box identification codes 7. In addition, the identification module 80 is also used to identify identification codes on the aforementioned plurality of storage components 5, such as the first identification code 513, the second identification code 5292, the third identification code 533, the fourth identification code 543, etc., to obtain information about the fluid within the corresponding storage component 5.

[0083] The biochemical reaction apparatus 100 provided in this application, firstly, integrates a fluid storage module 10, a pipetting module 20, a first biochemical reaction module 30, a second biochemical reaction module 40, and a preparation and mixing module 50 into a single device. This enables the automatic execution of pre-reaction steps in biochemical reactions, such as sample loading, mixing, and sample preparation (e.g., preparation of amplification products). Furthermore, the prepared sample can be automatically loaded into the second biochemical reaction module 40 for analysis and detection. This effectively improves the integration and automation of the biochemical reaction apparatus 100, reduces manual operation, effectively avoids the inconsistency issues introduced by manual operation, and enhances the user experience. In addition, the various modules in this biochemical reaction apparatus 100 have a compact structure and do not require external independent instruments, reducing the cost of purchasing additional equipment and the space required in the laboratory.

[0084] Secondly, in the fluid storage module 10, multiple storage components 5 are integrated into the first storage unit 2, concentrating various fluids in the same storage box. This fluid storage module 10 can be integrated into the biochemical reaction device 100 and, in conjunction with the pipetting module 20, achieves centralized scheduling and transfer of fluids, improving the efficiency and convenience of fluid transfer. Furthermore, the fluid storage module 10 has a reasonable layout and high space utilization, improving the integration and automation level of the biochemical reaction device 100. Additionally, by setting a temperature control structure 3 at one end of the first storage cavity 11, local temperature control can be achieved. Only individual temperature-sensitive reagents need temperature control, eliminating the need to control the entire fluid storage module 10. The temperature control structure 3 is compact, occupies little space, and can be directly integrated into the fluid storage module 10. This temperature control structure 3 has low energy consumption, reducing power supply requirements and overall power consumption, and effectively reducing the amount of condensate, thus reducing mold growth caused by condensate.

[0085] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit it. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the spirit and scope of the technical solutions of this application.

Claims

1. A biochemical reaction apparatus, characterized in that, include: A fluid storage module includes a first storage unit, the first storage unit including a first storage box, and a first storage component and a second storage component detachably disposed in the first storage box, the first storage component and the second storage component being used to store fluids required for biochemical reactions; A pipetting module is located above the fluid storage module; A first biochemical reaction module is connected to the pipetting module, and the first biochemical reaction module is used to perform a first biochemical reaction; A second biochemical reaction module is connected to the pipetting module and the first biochemical reaction module, and the second biochemical reaction module is used to perform a second biochemical reaction; and A mixing module is integrated into the fluid storage module. The mixing module is connectable to the first storage component and is used to mix the fluid in the first storage component. The pipetting module is used to transfer fluid from the first storage component to the first biochemical reaction module to perform the first biochemical reaction and obtain reaction products; the pipetting module is also used to transfer the reaction products and fluid from the second storage component to the second biochemical reaction module to perform the second biochemical reaction.

2. The biochemical reaction apparatus as described in claim 1, characterized in that, The first storage box includes a first storage compartment, the first storage component includes a first storage element detachably disposed in the first storage compartment, and the fluid storage module further includes a temperature control structure located in the first storage compartment and used to control the temperature of the fluid in the first storage element.

3. The biochemical reaction apparatus as described in claim 2, characterized in that, The temperature control structure includes a temperature conduction component, an auxiliary temperature control component, and a temperature control component. The temperature conduction component is disposed on the auxiliary temperature control component, and the temperature control component is located between the temperature conduction component and the auxiliary temperature control component. The temperature conduction component can contact the first storage device.

4. The biochemical reaction apparatus as described in claim 3, characterized in that, The first storage position is located at one end of the first storage box, the auxiliary temperature control component is located outside the first storage box, the temperature conduction component is located below the first storage position, and the temperature conduction component has a temperature control hole, and the first storage component is detachably disposed at the temperature control hole.

5. The biochemical reaction apparatus as described in claim 1, characterized in that, The first storage box further includes a second storage position, and a clearance position is provided below the second storage position. The preparation and mixing module is disposed in the clearance position. The first storage component further includes a second storage element that is detachably disposed in the second storage position. The second storage element is detachably connected to the preparation and mixing module.

6. The biochemical reaction apparatus as described in claim 5, characterized in that, The second storage component includes a limiting part and a plurality of mixing containers detachably disposed within the limiting part. The limiting part is detachably connected to the first storage position. Each mixing container includes a hollow container body, a locking part located at the bottom of the container body, and a protrusion disposed on the inner wall of the container body. The locking part can lock onto the preparation mixing module.

7. The biochemical reaction apparatus as described in claim 5, characterized in that, The first storage box further includes a third storage compartment, and the first storage component further includes a third storage element detachably disposed in the third storage compartment, wherein the holes of the second storage element and the holes of the third storage element are arranged side by side.

8. The biochemical reaction apparatus as described in claim 7, characterized in that, The first storage box further includes a cleaning tank and two cleaning holes disposed in the cleaning tank. The two cleaning holes are respectively located at the same end of the second storage position and the third storage position. The cleaning holes are used to clean the liquid picking needle of the pipetting module, and the cleaning tank is used to contain cleaning waste liquid.

9. The biochemical reaction apparatus as described in claim 1, characterized in that, The fluid storage module further includes a storage structure, which includes a mounting frame and a storage frame slidably disposed on the mounting frame. The storage frame forms a first storage cavity, and the first storage unit is located inside the first storage cavity. The storage structure also includes a locking element and a retaining element. The locking element is used to lock or unlock the storage frame, and the retaining element is used to provide auxiliary holding force for the storage frame after it is locked, and to provide feedback on the storage frame being locked in place.

10. The biochemical reaction apparatus as described in claim 1, characterized in that, The storage structure further includes a second storage cavity, and the fluid storage module further includes a second storage unit detachably disposed in the second storage cavity, the second storage unit being used to store cleaning fluid and waste fluid.

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