Functional peptide-rich proteolysis biotransformation reaction device

Abstract

The present application relates to the technical field of biological enzymatic processing equipment, and discloses a functional peptide-rich protease enzymatic biological conversion reaction equipment, which comprises a fixing support, a reaction kettle is fixedly installed in the middle of the fixing support, a discharging pipe is fixedly installed at the top of the reaction kettle, a first motor is fixedly installed at the top of the discharging pipe, a hollow stirring shaft is fixedly installed at the driving end of the first motor and extends into the inside of the reaction kettle, a sealing shell is installed at the lower side of the inside of the reaction kettle, a movable body is movably installed in the inside of the sealing shell, a limiting transverse groove is formed in each of the four side edges of the movable body, and a limiting sliding block is movably installed in the inside of each limiting transverse groove. The present application is provided with the structure of uniform material distribution, self-adaptive reversing stirring, magnetic drive defoaming, dynamic anti-blocking filtration and material circulation reflux, which can eliminate the reaction dead zone and product inhibition, guarantee the stability of the enzymatic environment, protect the peptide activity, improve the enzymatic efficiency and product purity, and facilitate the industrialized continuous production.

Description

Technical Field

[0001] This invention relates to the field of bio-enzymatic hydrolysis processing equipment technology, specifically a functional peptide-rich protease hydrolysis biotransformation reaction device. Background Technology

[0002] Functional peptides are prepared by targeted hydrolysis of proteins with proteases. The resulting peptide products possess excellent physiological activity and are now widely used in food, health products, and other related fields. Currently, most peptide production uses conventional reactors for enzymatic hydrolysis. The feeding methods in conventional equipment are relatively simple, with proteases often added from the top at a single point, which easily leads to excessively high local enzyme concentrations and uneven hydrolysis. Furthermore, the fixed stirring method during enzymatic hydrolysis can easily create dead zones within the reactor, resulting in insufficient mixing of the protein raw materials and proteases, ultimately reducing the conversion efficiency of the peptide products and limiting overall production effectiveness.

[0003] During enzymatic hydrolysis, the slurry is easily agitated and generates a large amount of foam. Existing production equipment generally eliminates foam by adding chemical defoamers, but the addition of defoamers can adversely affect the bioactivity of functional peptides. A few devices with built-in mechanical defoaming structures often have rigidly fixed defoaming components that cannot adapt to changes in liquid level, resulting in limited defoaming effectiveness. Furthermore, enzymatic hydrolysis is a reversible catalytic reaction, and the generated small-molecule peptide-rich products remain in the reaction system for a long time, easily causing product inhibition effects, hindering further protein hydrolysis, and prolonging the overall reaction time.

[0004] While some reactors currently incorporate filtration and separation structures, the filtration components are mostly statically installed. Protein impurities in the material easily clog the filter pores, leading to decreased filtration efficiency and hindering continuous operation for the separation of small-molecule peptides and the recycling of large-molecule materials. Furthermore, material deposits and scale easily accumulate on the inner wall of the reactor, affecting heat exchange efficiency, causing localized overheating, and further reducing the quality of the peptide products. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a functional peptide-rich enzymatic hydrolysis bioconversion reaction device, which solves problems such as uneven feeding, easy foaming, dead zones in stirring, inhibition of small molecule peptide products, easy clogging of filter cartridges, and scaling on the reactor wall in existing peptide-rich enzymatic hydrolysis devices, thereby improving the protein hydrolysis conversion efficiency.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solution: a functional peptide-rich protease hydrolysis biotransformation reaction device, comprising a fixed support, a reaction vessel fixedly installed in the middle of the fixed support, a feed pipe fixedly installed at the top of the reaction vessel, a first motor fixedly installed at the top of the feed pipe, and a hollow stirring shaft fixedly installed at the drive end of the first motor extending into the interior of the reaction vessel.

[0007] Preferably, a sealed shell is installed on the lower inner side of the reactor, and a movable body is movably installed inside the sealed shell. Each of the four side edges of the movable body has a limiting transverse groove, and a limiting slider is movably installed inside each limiting transverse groove. A reversing shaft is movably installed on each of the four outer sides of the sealed shell. A rotating disk is fixedly installed on the inner end of each reversing shaft. A pin is fixedly installed on one side of the inner end of each rotating disk, and the end of each pin extends into the central through hole of the corresponding limiting slider. An inclined blade stirring paddle is fixedly installed on the outer end of each reversing shaft.

[0008] Preferably, a bottom filter chamber is fixedly installed at the bottom of the reactor, a fixed circular plate is fixedly installed on the upper side of the inside of the bottom filter chamber, and an annular microporous filter cartridge is movably installed at the bottom of the fixed circular plate.

[0009] Preferably, a protease vacuum feeding station is fixedly installed on one side of the top of the fixed bracket, and a feeding pipe is fixedly installed on the top of the protease vacuum feeding station, with the end of the feeding pipe extending into the interior of the unloading pipe.

[0010] Preferably, a plurality of inclined stirring rods are fixedly installed on both sides of the hollow stirring shaft, an inverted conical baffle ring is fixedly installed inside the feeding pipe, a plurality of openings are provided on the outer wall of the hollow stirring shaft near the upper position of the inverted conical baffle ring, and a plurality of material dispensing ports are provided at the bottom of each inclined stirring rod.

[0011] Preferably, a floating aluminum ring is provided inside the reactor near the upper outer diameter of the hollow stirring shaft, and several serrated defoaming impellers are uniformly fixed on the outer diameter of the floating aluminum ring. A magnetic ring is fixedly installed on the upper outer diameter of the hollow stirring shaft near the floating aluminum ring.

[0012] Preferably, a cylindrical groove is formed at the center of the top of the movable body, and a keyway is formed on the inner side wall of the cylindrical groove. The end of the hollow stirring shaft extends into the interior of the cylindrical groove. Splines are fixedly installed on both sides of the bottom of the hollow stirring shaft, and the splines are movably arranged inside the keyway on the corresponding side. A push cylinder is fixedly installed at the bottom of the sealed housing, and the drive end of the push cylinder is fixedly installed at the bottom of the movable body.

[0013] Preferably, a second motor is fixedly installed at the bottom end of the bottom filter chamber. The drive end of the second motor extends into the interior of the bottom filter chamber and is fixedly installed with an output shaft. Two sets of cylinders are fixedly installed on the outer diameter of the output shaft. Inclined ball grooves are opened on the outer diameter of each cylinder, and inclined bearing seats are movably installed on the outer diameter of each inclined ball groove. One end of each inclined bearing seat is connected to the inner wall of the annular microporous filter cartridge through a torsion beam. A spring is fixedly installed in the middle of each torsion beam. A discharge branch pipe is fixedly installed on one side of the bottom of the annular microporous filter cartridge, and the end of the discharge branch pipe extends to the outside of the bottom filter chamber.

[0014] Preferably, a reducer is installed in the middle of the fixed circular plate and the end of the output shaft is fixedly installed at the bottom input end of the reducer. A rotating shaft is fixedly installed at the top output end of the reducer and wall scrapers are fixedly installed on both sides of the rotating shaft. One side of the bottom filter chamber is connected to the upper inside of the reactor through a reflux pipe and a circulation pump is installed in the middle of the reflux pipe.

[0015] Preferably, a feed port is provided on one side of the top of the reactor, an auxiliary material addition port is provided on the other side of the top of the reactor, a pressure stabilizing valve is also installed on the top of the reactor, and a discharge valve is fixedly installed on one side of the bottom of the reactor.

[0016] Preferably, an electrical control box is fixedly installed on one side of the front end of the fixed bracket, and a heat exchange jacket is fixedly installed on the outer wall of the reactor.

[0017] This invention provides a functional peptide-rich protease hydrolysis biotransformation reaction device. It has the following beneficial effects: 1. By setting up a vacuum feeding and hollow shaft dispensing structure, the present invention can achieve uniform feeding of protease, eliminate the problem of local enzyme concentration exceeding the standard caused by single-point feeding, and ensure that the enzymatic hydrolysis reaction proceeds uniformly and stably.

[0018] 2. This invention is equipped with a magnetically driven floating aluminum ring and a serrated defoaming paddle. It achieves low-speed rotation defoaming by relying on electromagnetic induction, which efficiently cuts the reaction foam, reduces the use of defoaming agents, and protects the bioactivity of peptides.

[0019] 3. The inclined blade stirring paddle of the present invention can automatically change direction according to the stirring direction, and in conjunction with the timed rotation switching of the motor, it breaks the fixed flow direction of the liquid, eliminates the dead zone of flow in the reactor, and greatly improves the overall stirring and mixing efficiency.

[0020] 4. This invention features a bottom filtration and circulation reflux structure, which can separate small molecule peptide-rich products from large molecule raw materials, eliminate product inhibition effects, effectively increase the upper limit of protein hydrolysis, and shorten the enzymatic reaction time. At the same time, the annular microporous filter cartridge can achieve reciprocating oscillation resonance, which can automatically offset the liquid filtration load, effectively prevent filter cartridge clogging, continuously ensure equipment filtration efficiency, and is suitable for long-term continuous enzymatic hydrolysis operations. Attached Figure Description

[0021] Figure 1 This is a perspective view of the present invention; Figure 2 This is a schematic diagram of the internal structure of the reactor in this invention; Figure 3 This is a schematic diagram of the hollow stirring shaft in this invention; Figure 4 This is a schematic diagram of the internal structure of the sealed housing in this invention; Figure 5for Figure 4 Enlarged view of point A in the middle; Figure 6 This is a schematic diagram of the internal structure of the bottom filter chamber in this invention; Figure 7 This is a schematic diagram of the structure of the annular microporous filter cartridge in this invention; Figure 8 This is a front view of the present invention.

[0022] The components are as follows: 1. Fixed support; 2. Reactor; 3. Feed pipe; 4. First motor; 5. Hollow stirring shaft; 6. Inclined stirring rod; 7. Protease vacuum feeding station; 8. Feed pipe; 9. Inverted conical baffle ring; 10. Opening; 11. Distributor feeding port; 12. Floating aluminum ring; 13. Serrated defoaming impeller; 14. Magnetic ring; 15. Sealed shell; 16. Moving body; 17. Limiting transverse groove; 18. Limiting slider; 19. Reversing shaft; 20. Rotating disk; 21. Pin shaft; 22. Inclined blade stirring impeller. ; 23. Cylindrical groove; 24. Spline; 25. Push cylinder; 26. Bottom filter chamber; 27. Fixed circular plate; 28. Annular microporous filter cartridge; 29. ​​Second motor; 30. Output shaft; 31. Cylinder body; 32. Inclined bearing seat; 33. Torsion beam; 34. Spring; 35. Discharge branch pipe; 36. Reducer; 37. Wall-mounted scraper; 38. Circulating pump; 39. Return pipe; 40. Feed port; 41. Auxiliary material addition port; 42. Pressure stabilizing valve; 43. Discharge valve; 44. Electrical control box; 45. Heat exchange jacket. Detailed Implementation

[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] Example: Please see the appendix Figure 1 -Appendix Figure 8 This invention provides a functional peptide-rich protease hydrolysis biotransformation reaction device, such as... Figure 1As shown, the system includes a fixed support 1, a reaction vessel 2 fixedly mounted in the middle of the fixed support 1, a feed pipe 3 fixedly mounted at the top of the reaction vessel 2, a first motor 4 fixedly mounted at the top of the feed pipe 3, and a hollow stirring shaft 5 fixedly mounted at the drive end of the first motor 4 into the interior of the reaction vessel 2. The fixed support 1 serves as the supporting base for the entire equipment, stably supporting the reaction vessel 2 and various functional components at the top and bottom, ensuring the overall stability of the equipment operation and preventing equipment shaking during operation from affecting the accuracy of the enzymatic hydrolysis reaction. The reaction vessel 2 is the core reaction chamber for the enzymatic hydrolysis and biotransformation of protein slurry, providing a sealed and stable reaction space for protein structure deconstruction and protease hydrolysis. The feed pipe 3 connects the feeding structure to the cavity of the reaction vessel 2, enabling precise introduction of protease material. The first motor 4 provides the core power for the operation of the internal stirring structure, driving the hollow stirring shaft 5 to rotate continuously, providing power support for the mixing and uniform advancement of the enzymatic hydrolysis reaction within the vessel.

[0025] In this embodiment, several inclined stirring rods 6 are fixedly installed on both sides of the hollow stirring shaft 5, and an inverted conical baffle ring 9 is fixedly installed inside the feed pipe 3. Several openings 10 are opened on the outer wall of the hollow stirring shaft 5 near the upper part of the inverted conical baffle ring 9, and several dispensing ports 11 are opened at the bottom of each inclined stirring rod 6. The hollow stirring shaft 5 adopts a hollow structure and forms an integrated dispensing channel with the openings 10 and dispensing ports 11. With the guiding and limiting effect of the inverted conical baffle ring 9, the introduced complex protease can be buffered and diverted, preventing the protease from directly falling into the material in the reactor. During the rotation of the inclined stirring rods 6, the protease can be evenly released into the reactor 2 through multiple dispensing ports 11, completely eliminating the problem of excessively high local enzyme concentration caused by traditional single-point feeding, allowing the protease to be fully mixed with the protein slurry, ensuring that the enzymatic hydrolysis reaction is uniform and stable throughout the process, and improving the overall bioconversion effect.

[0026] Furthermore, a sealed shell 15 is installed on the lower side of the reactor 2. A movable body 16 is movably installed inside the sealed shell 15. Limiting grooves 17 are formed on each of the four side edges of the movable body 16. Limiting sliders 18 are movably installed inside each of the limiting grooves 17. Reversing shafts 19 are movably installed on all four outer sides of the sealed shell 15. A rotating disk 20 is fixedly installed on the inner end of each reversing shaft 19. A pin 21 is fixedly installed on one side of the inner end of each rotating disk 20, with the end of each pin extending into the central through hole of the corresponding limiting slider 18. An inclined blade agitator 22 is fixedly installed on the outer end of each reversing shaft 19. The sealed shell 15 provides a sealed protection for the internal reversing transmission structure, preventing material intrusion that could cause structural jamming or wear, and ensuring stable operation of the transmission components. The movable body 16, the limiting grooves 17, and the limiting sliders 18 form a linked limiting structure, which can accurately transmit displacement power. The rotating disk 20 rotates in conjunction with the pin 21, thereby driving the reversing shaft 19 to complete an angle rotation. This linkage structure can be coordinated with the rotation switching of the stirring shaft to achieve adaptive reversing adjustment of the inclined blade stirring paddle 22, so that the stirring blades always maintain the stirring state with the material, effectively improving the stirring and mixing efficiency of the material in the reactor.

[0027] Furthermore, a bottom filter chamber 26 is fixedly installed at the bottom of the reactor 2. A fixed circular plate 27 is fixedly installed on the upper side of the interior of the bottom filter chamber 26, and an annular microporous filter cartridge 28 is movably installed at the bottom of the fixed circular plate 27. The bottom filter chamber 26 serves as the core cavity for product separation and material circulation, receiving the mixture from the enzymatic hydrolysis reaction in the reactor 2, achieving the fractional separation of small molecule products and large molecule raw materials. The fixed circular plate 27 provides fixed support and material diversion isolation, stabilizing the installation position of the annular microporous filter cartridge 28 and ensuring the overall stability of the filtration structure. The annular microporous filter cartridge 28 has a precise sieving function, capable of retaining incompletely reacted large molecule protein particles and proteases, while allowing small molecule functional peptides generated in the reaction to pass through smoothly, achieving effective separation of products and raw materials and eliminating the negative impact of product inhibition on the enzymatic hydrolysis reaction.

[0028] Furthermore, a protease vacuum feeding station 7 is fixedly installed on one side of the top of the fixed bracket 1. A feeding pipe 8 is fixedly installed on the top of the protease vacuum feeding station 7, with its end extending into the interior of the discharge pipe 3. The protease vacuum feeding station 7 utilizes the principle of vacuum negative pressure to achieve automated, sealed feeding of the protease, effectively preventing the protease material from being exposed to air and becoming damp or contaminated, thus ensuring the biological activity of the protease. The feeding pipe 8, as a material conveying channel, precisely connects the vacuum feeding station 7 and the discharge pipe 3, achieving stable and continuous conveying of the protease material. This replaces the traditional manual feeding method, not only improving the accuracy of the feeding ratio but also enhancing the automation level of the equipment, adapting to the needs of industrial continuous enzymatic hydrolysis production.

[0029] Furthermore, a floating aluminum ring 12 is installed inside the reactor 2 near the upper outer diameter of the hollow stirring shaft 5. Several serrated defoaming paddles 13 are uniformly fixed on the outer diameter of the floating aluminum ring 12. A magnetic ring 14 is fixedly installed near the floating aluminum ring 12 on the upper outer diameter of the hollow stirring shaft 5. The magnetic ring 14 rotates synchronously with the hollow stirring shaft 5, continuously changing the magnetic flux around the floating aluminum ring 12. Based on the principle of electromagnetic induction, this induces an Ampere force in the floating aluminum ring 12, thereby driving it to rotate at a low speed. The floating aluminum ring 12 always floats above the material surface, driving the uniformly distributed serrated defoaming paddles 13 to operate synchronously. The serrated structure efficiently cuts and breaks up the foam generated during the enzymatic hydrolysis reaction. This electromagnetic non-contact defoaming structure eliminates the need for defoaming agents, maximizing the protection of the bioactivity of functional peptides, avoiding interference with product quality from chemical additives, and reducing production material costs.

[0030] Furthermore, a cylindrical groove 23 is formed at the center of the top of the movable body 16, and a keyway is formed on the inner side wall of the cylindrical groove 23. The end of the hollow stirring shaft 5 extends into the interior of the cylindrical groove 23. Splines 24 are fixedly installed on both sides of the bottom of the hollow stirring shaft 5, and the splines 24 are movably disposed inside the corresponding side keyways. A push cylinder 25 is fixedly installed on the inner bottom of the sealing housing 15, and the drive end of the push cylinder 25 is fixedly installed on the bottom of the movable body 16. The hollow stirring shaft 5 forms a precise spline transmission structure through the splines 24 and the keyways of the movable body 16, realizing stable torque transmission. When the hollow stirring shaft 5 rotates, it can synchronously drive the sealing housing 15 and the inclined blade stirring paddle 22 to rotate, ensuring synchronous output of stirring power. As a displacement driving component, the push cylinder 25 can precisely push the moving body 16 to move up and down reciprocally, driving the limit slider 18 to slide within the limit groove 17, providing stable power for the subsequent reversing and flipping of the inclined blade agitator 22, realizing the automatic reversing adjustment of the agitation structure, and adapting to the operational needs of different agitation directions.

[0031] Furthermore, a second motor 29 is fixedly installed at the bottom end of the bottom filter chamber 26. The drive end of the second motor 29 extends into the interior of the bottom filter chamber 26 and is fixedly installed with an output shaft 30. Two sets of cylinders 31 are fixedly installed on the outer diameter of the output shaft 30. Each cylinder 31 has an inclined ball groove on its outer diameter, and a slanted bearing seat 32 is movably installed on the outer diameter of each inclined ball groove. One end of each slanted bearing seat 32 is connected to the inner wall of the annular microporous filter cartridge 28 through a torsion beam 33. A spring piece 34 is fixedly installed in the middle of each torsion beam 33. A discharge branch pipe 35 is fixedly installed on one side of the bottom of the annular microporous filter cartridge 28, and the end of the discharge branch pipe 35 extends to the outside of the bottom filter chamber 26. The second motor 29 provides power to the bottom filter vibration structure, drives the output shaft 30 to rotate continuously, and drives the cylinders 31 to rotate synchronously. The inclined ball groove on the outer periphery of the cylinder 31 drives the inclined bearing seat 32 to reciprocate. Combined with the torsional energy storage and rebound characteristics of the torsion beam 33 and the spring plate 34, it drives the annular microporous filter cartridge 28 to continuously swing up and down and generate a resonance effect. This structure can effectively offset the pressure load on the filter cartridge during material filtration, prevent micropore blockage, continuously ensure filtration throughput, and the separated small molecule peptide-rich products can be stably discharged from the equipment through the discharge branch pipe 35 to complete product collection.

[0032] Furthermore, a reducer 36 is installed in the middle of the fixed circular plate 27, and the end of the output shaft 30 is fixedly installed at the bottom input end of the reducer 36. A rotating shaft is fixedly installed at the top output end of the reducer 36, and wall-mounted scraper plates 37 are fixedly installed on both sides of the rotating shaft. One side of the bottom filter chamber 26 is connected to the upper interior of the reactor 2 through a return pipe 39, and a circulation pump 38 is installed in the middle of the return pipe 39. The reducer 36 can reduce and adjust the rotation speed of the output shaft 30 to provide a suitable rotation speed for the wall-mounted scraper plates 37, avoiding material splashing due to excessive speed and incomplete scraping due to excessively slow speed. The wall-mounted scraper plates 37 can scrape off the protein material adhering to the inner wall of the reactor 2 and the bottom filter chamber 26 in real time, effectively preventing scaling on the heat exchange surface and inner wall, avoiding local overheating and deterioration of the material, and reducing the difficulty of cleaning the equipment. The circulating pump 38 provides power for material reflux, which can transport the large molecular proteins and unreacted proteases retained in the bottom filter chamber 26 back to the reactor 2 through the reflux pipe 39 to participate in the enzymatic hydrolysis reaction again, thereby realizing material recycling and improving the utilization rate of raw materials and the protein hydrolysis conversion rate.

[0033] Furthermore, a feed port 40 is provided on one side of the top of reactor 2, and an auxiliary material addition port 41 is provided on the other side of the top of reactor 2. A pressure regulating valve 42 is also installed on the top of reactor 2, and a discharge valve 43 is fixedly installed on one side of the bottom of reactor 2. The feed port 40 is used to accurately pump the pretreated protein slurry into the reactor 2 to complete the raw material feeding operation; the auxiliary material addition port 41 can add pH adjusters, buffers and other auxiliary materials as needed to precisely control the enzymatic hydrolysis reaction environment. The pressure regulating valve 42 can balance the internal air pressure of reactor 2 in real time to avoid excessive pressure inside the reactor affecting the reaction stability and ensure the safety of equipment operation. The discharge valve 43 is used to uniformly discharge the mixed slurry in the reactor after the enzymatic hydrolysis reaction is completed, realizing the controllable operation of material entry and exit, and adapting to standardized and regulated production processes.

[0034] Furthermore, an electrical control box 44 is fixedly installed on one side of the front end of the fixed bracket 1, and a heat exchange jacket 45 is fixedly installed on the outer wall of the reactor 2. The electrical control box 44, as the core control hub of the equipment, can centrally regulate all operating procedures such as motor operation, stirring speed, temperature control, and circulation filtration, achieving automated integrated control of the equipment and simplifying manual operation. The heat exchange jacket 45 can achieve heating, heat preservation, and cooling operations through water circulation, precisely matching process requirements. It first uses high temperature to destroy the higher-order spatial structure of proteins and expose peptide bond sites, then cools down to lock in the optimal reaction temperature of the protease, precisely controlling the enzymatic hydrolysis reaction conditions and significantly improving the efficiency of biotransformation reactions and product quality.

[0035] Working principle: The treated protein slurry is pumped into the reactor 2 through the feed port 40. The heat exchange jacket 45 introduces a heat source to start heating. After reaching the preset temperature, it is kept at this temperature for a period of time. The high temperature destroys the higher spatial structure of the protein, exposing peptide bond sites, which facilitates subsequent protease shearing and hydrolysis. Then, it is cooled down to the suitable reaction temperature for the protease. The first motor 4 is started, which drives the hollow stirring shaft 5 to rotate, thereby driving the inclined stirring rod 6 to rotate. At the same time, under the limiting effect of the spline 24 and the keyway, the sealed shell 15 is driven to rotate, thereby driving the inclined blade stirring paddle 22 to rotate. At this time, the pH adjuster and other excipients are added through the excipient addition port 41. The heat exchange jacket 45 uses water circulation to control the temperature and lock the optimal reaction temperature for enzymatic hydrolysis. Then, according to the ratio, the compound protease is introduced through the protease vacuum feeding station 7 and the feeding pipe 8. The protease enters the interior of the hollow stirring shaft 5 through the opening 10 through the inverted conical baffle ring 9, and then enters the reactor 2 evenly through the feeding port 11 at the bottom of each inclined stirring rod 6 to start the biotransformation enzymatic hydrolysis reaction, avoiding single-point feeding. Local enzyme concentration exceeds the standard, and the floating aluminum ring 12 always floats on the liquid surface. When the hollow stirring shaft 5 rotates, it drives the magnetic ring 14 to rotate. When the magnetic ring 14 rotates, it continuously changes the magnetic flux of the floating aluminum ring 12. Based on the principle of electromagnetic induction, the floating aluminum ring 12 generates an induced Ampere force, which drives the floating aluminum ring 12 to rotate at a low speed following the direction of the magnetic field. This drives all the sawtooth defoaming paddles 13 to rotate as well. The sawtooth structure defoaming paddles efficiently cut the foam generated during the reaction, reducing the amount of defoamer added and ensuring that the bioactivity of the peptide is not interfered with by additives. The first motor 4 switches the stirring direction at regular intervals to break the fixed flow direction of the liquid and eliminate the dead zone in the tank. When the first motor 4 switches the stirring direction, it pushes the cylinder 25 to drive the moving body 16 to move, which in turn drives all the limit sliders 18 to move. When the limit sliders 18 move, they drive the pin shaft 21 to move as well. The movement of the pin shaft 21 drives the rotating disk 20 to rotate, which in turn drives the reversing shaft 19 and the inclined blade stirring paddle 22 to flip. This allows the inclined blade stirring paddle 22 to automatically flip in the same direction as the stirring direction, improving the stirring efficiency.

[0036] During the reaction, small-molecule functional peptide precipitates are continuously generated. These small-molecule peptides enter the annular microporous filter cartridge 28 and are then discharged through the discharge branch pipe 35. Large-molecule protein particles and proteases are retained in the bottom filter chamber 26 and returned to the reactor 2 via the circulation pump 38 and reflux pipe 39 to continue participating in the catalytic reaction, eliminating product inhibition by small-molecule peptides, increasing the upper limit of hydrolysis, and shortening the enzymatic hydrolysis time. Simultaneously, the second motor 29 is started, driving the output shaft 30 to rotate, which in turn drives all cylinders 31 to rotate. Because there is a certain angle between the ball groove on the outer diameter of the cylinder 31 and the central axis of the cylinder 31, when the cylinder 31 rotates continuously, it will drive the inclined bearing seat 32 to oscillate back and forth. When the inclined bearing seat 32 oscillates, it will... One end of the torsion beam 33 swings, storing and transmitting torque to the other end of the torsion beam 33, thereby causing the annular microporous filter cartridge 28 to swing up and down, avoiding clogging during filtration. As the swing frequency of the inclined bearing seat 32 increases, the swing of the two ends of the torsion beam 33 gradually becomes synchronized, and the resulting resonance increases the torque of the torsion beam 33, effectively counteracting the load applied by the liquid to the vibrating annular microporous filter cartridge 28, improving the vibration effect, and thus improving the continuous filtration efficiency. At the same time, when the output shaft 30 rotates, it will also drive the scraper plate 37 to rotate through the action of the reducer 36, scraping off the raw materials adhering to the inner wall of the reactor 2 in real time, avoiding scaling and local overheating on the heat exchange surface, and also facilitating subsequent cleaning work. Finally, the discharge valve 43 is opened to discharge the internal slurry.

[0037] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A functional peptide-rich protease hydrolysis biotransformation reaction device, comprising a fixed support (1), characterized in that, The fixed support (1) has a reactor (2) fixedly installed in the middle, and a feed pipe (3) is fixedly installed at the top of the reactor (2). A first motor (4) is fixedly installed at the top of the feed pipe (3). The drive end of the first motor (4) extends into the interior of the reactor (2) and a hollow stirring shaft (5) is fixedly installed thereon. A sealing shell (15) is installed on the lower side of the reactor (2). A movable body (16) is movably installed inside the sealing shell (15). Limiting grooves (17) are opened on the four side edges of the movable body (16). Limiting sliders (18) are movably installed inside the limiting grooves (17). A reversing shaft (19) is movably installed on the four sides of the outer side of the sealing shell (15). A rotating disk (20) is fixedly installed on the inner end of the reversing shaft (19). A pin (21) is fixedly installed on one side of the inner end of the rotating disk (20), and the end of the pin (21) extends into the central through hole of the corresponding limiting slider (18). An inclined blade stirring paddle (22) is fixedly installed on the outer end of the reversing shaft (19). The bottom of the reactor (2) is fixedly installed with a bottom filter chamber (26), and a fixed circular plate (27) is fixedly installed on the upper side of the inside of the bottom filter chamber (26). An annular microporous filter cartridge (28) is movably installed at the bottom of the fixed circular plate (27).

2. The functional peptide-rich protease hydrolysis biotransformation reaction device according to claim 1, characterized in that, A protease vacuum feeding station (7) is fixedly installed on one side of the top of the fixed bracket (1), and a feeding pipe (8) is fixedly installed on the top of the protease vacuum feeding station (7), with the end of the feeding pipe (8) extending into the interior of the unloading pipe (3).

3. The functional peptide-rich protease hydrolysis biotransformation reaction device according to claim 1, characterized in that, Several inclined stirring rods (6) are fixedly installed on both sides of the hollow stirring shaft (5). An inverted conical baffle ring (9) is fixedly installed inside the feed pipe (3). Several openings (10) are opened on the outer wall of the hollow stirring shaft (5) near the upper position of the inverted conical baffle ring (9). Several material dispensing ports (11) are opened at the bottom of the inclined stirring rods (6).

4. The functional peptide-rich protease hydrolysis biotransformation reaction device according to claim 1, characterized in that, A floating aluminum ring (12) is provided inside the reactor (2) near the upper outer diameter of the hollow stirring shaft (5). Several sawtooth defoaming paddles (13) are uniformly fixed on the outer diameter of the floating aluminum ring (12). A magnetic ring (14) is fixedly installed on the upper outer diameter of the hollow stirring shaft (5) near the floating aluminum ring (12).

5. The functional peptide-rich protease hydrolysis biotransformation reaction device according to claim 1, characterized in that, The top center of the movable body (16) is provided with a cylindrical groove (23) and the inner side wall of the cylindrical groove (23) is provided with a keyway. The end of the hollow stirring shaft (5) extends into the interior of the cylindrical groove (23). Splines (24) are fixedly installed on both sides of the bottom of the hollow stirring shaft (5) and the splines (24) are movably arranged inside the keyway on the corresponding side. A push cylinder (25) is fixedly installed on the inner bottom of the sealed housing (15) and the drive end of the push cylinder (25) is fixedly installed on the bottom end of the movable body (16).

6. The functional peptide-rich protease hydrolysis biotransformation reaction device according to claim 1, characterized in that, A second motor (29) is fixedly installed at the bottom end of the bottom filter chamber (26). The drive end of the second motor (29) extends into the interior of the bottom filter chamber (26) and is fixedly installed with an output shaft (30). Two sets of cylinders (31) are fixedly installed on the outer diameter of the output shaft (30). Inclined ball grooves are opened on the outer diameter of each cylinder (31), and inclined bearing seats (32) are movably installed on the outer diameter of each inclined ball groove. One end of each inclined bearing seat (32) is connected to the inner wall of the annular microporous filter cylinder (28) through a torsion beam (33). A spring piece (34) is fixedly installed in the middle of each torsion beam (33). A discharge branch pipe (35) is fixedly installed on one side of the bottom of the annular microporous filter cylinder (28), and the end of the discharge branch pipe (35) extends to the outside of the bottom filter chamber (26).

7. The functional peptide-rich protease hydrolysis biotransformation reaction device according to claim 6, characterized in that, A reducer (36) is installed in the middle of the fixed circular plate (27), and the end of the output shaft (30) is fixedly installed at the bottom input end of the reducer (36). A rotating shaft is fixedly installed at the top output end of the reducer (36), and wall scraping plates (37) are fixedly installed on both sides of the rotating shaft. One side of the bottom filter chamber (26) is connected to the upper inside of the reactor (2) through a return pipe (39), and a circulating pump (38) is installed in the middle of the return pipe (39).

8. The functional peptide-rich protease hydrolysis biotransformation reaction device according to claim 1, characterized in that, The reactor (2) has a feed port (40) on one side of the top and an auxiliary material addition port (41) on the other side of the top. The reactor (2) is also equipped with a pressure stabilizing valve (42) on the top and a discharge valve (43) on one side of the bottom.

9. The functional peptide-rich protease hydrolysis biotransformation reaction device according to claim 1, characterized in that, An electrical control box (44) is fixedly installed on one side of the front end of the fixed bracket (1), and a heat exchange jacket (45) is fixedly installed on the outer wall of the reactor (2).

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