A stress-resistant selenium-based quantum editing ecological regulation system and a construction method thereof

Abstract

The application discloses a kind of ecological regulation and control systems of selenium-based quantum editing resistance and construction method thereof, it is related to quantum technical field, including quantum entanglement preparation module, quantum state acquisition and analysis unit and artificial intelligence early warning control module;Through 532nm laser and 0.1T magnetic field preparation "on-site microorganism-gene editing reference bacteria" quantum entanglement pair, signal is collected using quantum state analyzer and is analyzed by exclusive algorithm Colony synergy parameter, when cooperation efficiency is below threshold value, AI model automatically instructs nano carrier target delivery organic selenium activator and adjusts environmental parameter, realizes "monitoring-control" closed loop, solve the problem that traditional microbial community monitoring "time is out, precision is low, regulation and control are disjointed", with the advantages of real-time non-invasive, high sensitivity, multi-scene adaptation, applicable to environmental microbial repair, industrial microbial control, medical microecology monitoring and other fields.

Description

Technical Field

[0001] This invention relates to the field of quantum technology, specifically to a resilient selenium-based quantum editing ecological regulation system and its construction method. Background Technology

[0002] Quantum entanglement and quantum dot technologies have advantages such as non-contact, high sensitivity, and rapid response. However, when applied to microbial monitoring, they face key challenges such as the susceptibility of quantum states to decoherence due to metabolic disturbances, the lack of dedicated decoding algorithms for quantum signals and synergistic parameters of the microbial community, and poor compatibility between quantum materials and biomolecules. Traditional biochips only have a single detection function and cannot achieve gene-level regulation. Standalone gene editing tools lack real-time monitoring and feedback mechanisms, resulting in insufficient editing accuracy and controllability.

[0003] Selenium, a key micronutrient essential for both humans and microorganisms, exhibits significant effects in its highly active organic selenium forms, such as methylselenocysteine ​​and selenomethionine, in terms of antioxidant activity, immune regulation, and enhancement of microbial metabolic activity. However, the conversion efficiency of inorganic selenium to highly active organic selenium is low, resulting in insufficient bioavailability. Furthermore, the lack of a chip-level technology system integrating quantum sensing, gene editing, targeted delivery, and intelligent regulation hinders the efficient regulation of microbial stress resistance and ecological stability.

[0004] Based on the above-mentioned shortcomings, this invention proposes an integrated system and construction method with an organic selenium functional chip as the core. Summary of the Invention

[0005] The purpose of this invention is to provide a stress-resistant selenium-based quantum editing ecological regulation system and its construction method, so as to solve the problem that traditional quantum dot chips in the prior art can only detect but cannot achieve gene-level regulation.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a resilient selenium-based quantum editing ecological regulation system, comprising:

[0007] Quantum entanglement preparation module, quantum state acquisition and analysis unit, and artificial intelligence early warning and control module;

[0008] The system uses an organic selenium functional chip as its core hardware carrier, which integrates selenium quantum dot sensing and gene editing functions.

[0009] The quantum entanglement preparation module, the quantum state acquisition and analysis unit, and the artificial intelligence early warning and control module are electrically connected and interact with signals through the chip's internal microcircuits and microfluidic channels.

[0010] The organic selenium functional chip is equipped with a selenium quantum dot array sensing unit, a gene editing execution unit, and a signal feedback unit.

[0011] Furthermore, the quantum entanglement preparation module includes a 532nm laser, a 0.1T magnetic field generator, a microfluidic sample unit, and a temperature control subunit.

[0012] Furthermore, the quantum state acquisition and analysis unit incorporates a quantum state analyzer, a quantum-microbe community collaborative decoding algorithm chip, and a data preprocessing module.

[0013] Furthermore, the artificial intelligence early warning and control module includes a machine learning early warning chip, an intervention instruction generation unit, and a nanocarrier targeted delivery control interface.

[0014] Furthermore, the selenium quantum dot array sensing unit is fixed to the chip substrate via EDC / NHS chemical coupling, with a coupling reaction pH of 6.0-7.0, and the selenium quantum dot surface is modified with a PVP coating with a thickness of 2-5 nm.

[0015] Furthermore, the gene editing execution unit is in situ fixed with a CRISPR-Cas9 protein component, and the gene editing execution unit is configured to adjust the activity of the Cas9 protein and the targeting of the editing site based on quantum signals.

[0016] A stress-resistant selenium-based quantum editing ecological regulation system is applied in environmental remediation, intestinal microecological regulation, and the industrial chain of highly active organic selenium.

[0017] A method for constructing a resilient selenium-based quantum editing ecological regulation system includes the following steps:

[0018] S1. Gene editing of key genes in the selenium metabolism pathway of engineered bacteria based on an organic selenium functional chip;

[0019] S2. Microbial samples and gene-edited engineered bacteria are placed in a magnetic field integrated with a chip and irradiated with a laser to prepare quantum entangled pairs in situ on an organoselenium functional chip.

[0020] S3. Collect quantum signals through the quantum sensing module of the organic selenium functional chip and analyze the signals into bacterial community synergy efficiency parameters;

[0021] S4. When the bacterial community synergy efficiency parameter is lower than the preset threshold, the chip drives the nanocarrier to deliver the organic selenium activator in a targeted manner.

[0022] Furthermore, the gene editing described in step S1 involves knocking out the recA gene of the engineered bacteria using CRISPR technology, and the gene editing process is tracked in real time by a selenium quantum dot fluorescent probe on the chip.

[0023] Furthermore, the magnetic field strength used in the quantum entanglement preparation process is 0.1T, and the laser wavelength is 532nm. The system automatically performs closed-loop control based on the collected quantum signals.

[0024] A method for constructing a stress-resistant selenium-based quantum editing ecological regulation system has applications in environmental remediation, gut microbiota regulation, and the high-activity organic selenium industry chain.

[0025] Compared with existing technologies, this invention provides a stress-resistant selenium-based quantum editing ecological regulation system and its construction method. It prepares quantum entangled pairs of "on-site microorganisms and gene-editing reference bacteria" using a 532nm laser and a 0.1T magnetic field. Signals are collected using a quantum state analyzer, and the community synergy parameters are analyzed using a dedicated algorithm. When the synergy efficiency is below a threshold, the AI ​​model automatically instructs a nanocarrier to target and deliver an organic selenium activator and adjust environmental parameters, achieving a closed-loop "monitoring-regulation" system. This solves the problems of "poor timeliness, low accuracy, and disconnected regulation" in traditional microbial community monitoring. It has the advantages of real-time non-invasiveness, high sensitivity, and multi-scenario adaptability, and is suitable for fields such as environmental microbial remediation, industrial microbial community regulation, and medical microecological monitoring. Detailed Implementation

[0026] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below.

[0027] Example 1:

[0028] (1) Organic selenium functional chip - quantum entanglement preparation module

[0029] Using an organic selenium functional chip as the core hardware carrier, the chip integrates a selenium quantum dot array sensing unit, a 532nm laser, a 0.1T magnetic field generator, a microfluidic sample culture unit, and a high-precision temperature control subunit. This module can fully mix on-site microbial samples and gene-edited engineered bacteria reference samples in a closed microfluidic channel and expose them to a stable physical field. By precisely controlling the laser parameters, magnetic field strength, and ambient temperature, stable quantum entanglement pairs are formed in situ inside the chip. The temperature control range is 25-37℃, which can maintain a microbial survival rate of ≥95% and a quantum entanglement half-life of ≥8h.

[0030] (2) Organic selenium functional chip - quantum state acquisition and analysis unit

[0031] The chip incorporates a quantum state analyzer, an integrated quantum-microbial community decoding algorithm chip, and a high-speed data preprocessing module; it is used for in-situ, non-invasive, real-time acquisition of entangled state signals, and real-time analysis of microbial community synergy efficiency parameters through selenium quantum dot optical signals, achieving high sensitivity, high precision, and real-time dynamic detection.

[0032] (3) Organic selenium functional chip - artificial intelligence early warning and control module

[0033] The chip integrates a machine learning early warning chip, an intelligent intervention instruction generation unit, and a nanocarrier targeted delivery control interface; it is used to output chip-level precise intervention instructions based on the collaborative efficiency parameters, drive the chip's microfluidic targeted delivery channel to precisely release organic selenium activator, and complete a fully automatic closed loop of detection-regulation-feedback integration.

[0034] The reference sample for the gene-edited engineered bacteria was the standard labeling strain for the organic selenium functional chip, specifically *E. coli* with the recA gene knocked out, at a concentration of 10-1. 6 -10 8 CFU / mL; on-site microbial sample concentration 10 6 -10 8 CFU / mL.

[0035] The core structure of the organic selenium functional chip:

[0036] Selenium quantum dot array: As a fluorescent tracking probe and core unit of quantum sensing, it is fixed to the chip substrate by EDC / NHS chemical coupling at pH 6.0-7.0. The surface is modified with a 2-5nm PVP coating, which can significantly reduce non-specific binding with Cas protein, reduce quantum dot aggregation rate by 80%, and retain more than 90% of Cas protein activity.

[0037] Gene editing execution unit: The chip immobilizes Cas9 protein in situ and can receive quantum signal feedback in real time to dynamically regulate editing activity, timing of action and site targeting.

[0038] Signal feedback unit: The chip has a built-in microcircuit system that converts the fluorescence intensity of selenium quantum dots into editing efficiency and microbial community synergy parameters in real time. When the parameters are below the threshold, the control process is automatically triggered.

[0039] The chip is fabricated using a silicon-based and PDMS composite substrate. Microcircuits and microfluidic channels are integrated through micro-nano fabrication technology to achieve stable connection and signal transmission between various functional modules. The overall system structure is compact and highly integrated, and can complete the entire process of quantum entanglement preparation, signal acquisition and analysis, and intelligent control in a miniaturized space.

[0040] Example 2:

[0041] (1) Organic selenium functional chip fabrication and integration

[0042] Microfluidic channels are fabricated using a silicon-PDMS composite substrate through micro-nano fabrication processes such as ultraviolet lithography and reactive ion etching. A selenium quantum dot array is covalently coupled to the chip's sensing area via EDC / NHS, and the surface is coated with a 3nm thick PVP coating to improve biocompatibility and quantum yield. The chip integrates a 532nm laser driving unit, a 0.1T micro magnetic field coil, and a high-precision temperature control module (temperature control accuracy ±0.1℃) to achieve stable temperature control within the range of 25-37℃, ensuring the physiological activity and quantum state stability of microorganisms.

[0043] (2) Processing of strains and microbial samples

[0044] The remediation microbial community (mainly containing acid-producing bacteria and selenium-reducing bacteria) of heavy metal contaminated soil was collected, and after serial dilution, centrifugation and resuspending, the concentration was adjusted to 5×10⁻⁶. 7 CFU / mL; the strain was labeled using an organoselenium functional chip, specifically *E. coli* with the recA gene knocked out by CRISPR-Cas9, and the concentration was adjusted to 5 × 10⁻⁶ using the same method. 7 CFU / mL was injected into the corresponding independent microfluidic channels of the chip to avoid cross-contamination.

[0045] (3) In-situ quantum entanglement preparation on a chip

[0046] The chip's magnetic field and laser unit were activated, and the chip was irradiated for 10 minutes under a constant magnetic field of 0.1T and a 532nm laser (10mW power). Stable quantum entanglement pairs were formed in situ within the microchannel of the organic selenium functional chip. The entanglement degree was measured to be 0.87 and the half-life was 11 hours, which meets the technical requirements for long-term continuous real-time monitoring.

[0047] Example 3:

[0048] (1) Chip quantum signal acquisition

[0049] The organic selenium functional chip quantum sensing module continuously acquires quantum entangled state signals at a sampling frequency of 50Hz, with a detection accuracy of ≤0.1Hz. The chip's built-in data preprocessing module filters out environmental noise, electromagnetic interference, and temperature drift to obtain a high signal-to-noise ratio raw signal, providing a reliable data foundation for subsequent parameter analysis.

[0050] (2) Chip algorithm to analyze bacterial community coordination parameters

[0051] The chip has a built-in dedicated decoding algorithm that converts quantum signals into three key collaborative parameters: organic selenium conversion efficiency, population abundance correlation, and functional gene expression synchronization. Among them, the population abundance correlation ranges from 0 to 1, with higher values ​​indicating closer cooperation and more stable metabolic functions within the bacterial community.

[0052] (3) Fully automatic closed-loop intervention execution of the chip

[0053] When the synergistic efficiency of the microbial community is detected to be lower than the threshold (30%), the feedback unit of the organic selenium functional chip is immediately and automatically triggered: driving the microfluidic targeted delivery unit to release 0.5 mmol / L organic selenium activator; the chip automatically adjusts the ambient temperature to 25-37℃ and the pH to 6.5-7.5; after continuous monitoring for 2 hours, the synergistic efficiency quickly recovers from 28% to 59%, fully verifying the high efficiency, stability and real-time performance of the chip-level closed-loop regulation.

[0054] Example 4:

[0055] Application of organic selenium functional chips in regulating the stress resistance of soil remediation microbial communities

[0056] 1) Sample preparation: Microbial communities were collected from soil contaminated with heavy metals Cd and Pb, mainly selenium-reducing bacteria and acid-producing bacteria. After serial dilution, centrifugation, and washing, the concentration was adjusted to 5 × 10⁻⁶. 7 CFU / mL was injected into the microfluidic channel of the organic selenium functional chip.

[0057] 2) Chip calibration: Using the matching gene-edited Escherichia coli as a reference strain, quantum entangled pairs were prepared in situ on the chip with an initial entanglement degree of 0.87 and a half-life of 11h, which met the monitoring conditions.

[0058] 3) Stress treatment: The pH of the soil suspension was artificially increased to simulate the environmental stress caused by acid-base changes in the natural environment. Real-time chip monitoring showed that the synergistic efficiency of the microbial community dropped rapidly to 28%, and the metabolic activity decreased significantly.

[0059] 4) Chip regulation: The organic selenium functional chip automatically initiates the targeted delivery process, controlling the 100nm liposome nanocarrier to deliver 0.5mmol / L organic selenium activator, which targets and binds to the cell membrane receptor of selenium-reducing bacteria to achieve precise activation.

[0060] To evaluate the regulatory effect of soil remediation microbial communities, the survival rate of microbial communities was determined by plate counting using an automated colony counter (SCAN-100). The conversion efficiency of organoselenium was determined by high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) using an Agilent 1260 HPLC system and an Agilent 7900 ICP-MS system. The removal rate of heavy metals was determined by flame atomic absorption spectrophotometry using a Shimadzu AA-6300C atomic absorption spectrophotometer. The quantum entanglement degree and half-life were determined by real-time quantum state monitoring using a quantum state analyzer (QSA-200). The targeted delivery efficiency of nanocarriers was determined by fluorescence labeling using a Thermo Multiskan FC microplate reader.

[0061] A total of 4 experimental groups were set up, and the treatment methods of each group were different as follows:

[0062] Initial state group: Initial samples of soil remediation microbial communities in heavy metal contaminated soil that were not subjected to environmental stress and were routinely cultured;

[0063] Pre-intervention stress group: Microbial community samples without any intervention after a sudden increase in soil pH;

[0064] Post-intervention treatment group: Microbial samples treated with organic selenium activator delivered by the nanocarrier of this system for 2 hours after a sudden increase in soil pH;

[0065] Blank control group: After a sudden increase in soil pH, only an equal amount of blank nanocarrier was applied to the bacterial community sample without organic selenium activator.

[0066] Table 1. Results of soil remediation microbial community intervention before and after intervention

[0067] detection indicators Initial state group Pre-intervention stress group Post-intervention treatment group Blank control group Survival rate of bacterial flora (%) 96.2 61.5 94.8 58.3 Organic selenium conversion efficiency (%) 62.0 27.6 59.2 25.1 Cd removal rate (%) 41.3 12.7 38.5 10.2 Pb removal rate (%) 36.8 9.4 34.1 7.6 Quantum entanglement degree half-life (h) 11.0 4.2 10.5 3.8 Nanocarrier delivery efficiency (%) — — 92.3 — Collaborative efficiency parameter (%) 71.0 28.0 59.0 26.5

[0068] 5) Effect detection: As shown in Table 1, after 2 hours of intervention, the chip real-time monitoring showed that the bacterial survival rate increased from 61.5% to 94.8%, the organic selenium conversion efficiency increased from 27.6% to 59.2%, the Cd removal rate increased from 12.7% to 38.5%, the Pb removal rate increased from 9.4% to 34.1%, and the chip targeted delivery efficiency reached 92.3%.

[0069] After being controlled by the artificial intelligence closed-loop regulation based on quantum signals, the survival rate of soil remediation microorganisms, the conversion efficiency of organic selenium and the removal rate of heavy metals were all restored to the initial level. The targeted delivery efficiency of the nanocarrier reached 92.3%, and the stability of the quantum entangled state was significantly improved. This proves that the system has a significant effect on the stress resistance regulation of soil remediation microorganisms and can quickly restore the metabolic function and remediation capacity of the microorganisms.

[0070] The stress-resistant selenium-based quantum editing ecological regulation system provided by this invention, when applied to the synergistic regulation of microbial communities in heavy metal-contaminated soil remediation, can rapidly respond to environmental stresses caused by a sudden increase in pH, precisely triggering the targeted delivery of organic selenium activators via nanocarriers. Testing showed that after intervention, the survival rate of the microbial community, the organic selenium conversion efficiency, and the removal rate of heavy metals (Cd, Pb) all recovered to their initial levels. The quantum entanglement degree and half-life significantly increased, the nanocarrier targeted delivery efficiency reached 92.3%, and the synergistic efficiency parameters rapidly recovered to the normal range. The blank control group showed no significant recovery effect, demonstrating that this system, through real-time quantum monitoring and closed-loop regulation using artificial intelligence, can significantly improve the stress resistance of soil remediation microbial communities, effectively maintain the stability of the community's synergistic function, and possesses excellent application value in soil ecological regulation.

[0071] The stress-resistant selenium-based quantum editing ecological regulation system and construction method of this invention can be further extended to the fields of livestock and poultry intestinal microecological regulation and high-activity organic selenium production chain. In the application of livestock and poultry intestinal microecological regulation, the native microbial community in the livestock and poultry intestine is used as the detection object. An organic selenium functional chip is used to non-invasively monitor the metabolic state and synergistic efficiency of the intestinal flora in real time. Quantum entanglement signals are used to analyze the abundance changes and functional stability of the intestinal flora. When the synergistic efficiency of the intestinal flora decreases due to stress, dietary changes, or environmental fluctuations, the system can automatically trigger the targeted delivery of organic selenium activators via nanocarriers, directionally regulating the activity of selenium-metabolizing functional bacteria in the intestine, optimizing the intestinal flora structure, enhancing the intestinal mucosal barrier and the body's stress resistance, maintaining the dynamic balance of the intestinal microecology, and providing a stable and efficient means of microecological regulation for healthy livestock and poultry farming.

[0072] In the application of the high-activity organic selenium industry chain, the organic selenium functional chip can be used to achieve precise gene editing and regulation of selenium metabolism pathways in plants or microorganisms. The selenium quantum dot sensing unit on the chip can monitor gene editing efficiency and organic selenium synthesis status in real time. Through chip closed-loop regulation, the conversion efficiency of inorganic selenium to highly active methylselenocysteine ​​and selenomethionine can be improved. Then, through chip-controlled extraction, purification, and nano-processing, highly stable and highly bioavailable organic selenium raw materials can be prepared. Applying this raw material to the large-scale production of functional Chinese medicine, health food, and other production chains can significantly improve the content and stability of organic selenium in the production chain, so that the final research and development products have higher efficacy, nutritional value, and application value. Thus, the system and construction method of this invention can be comprehensively applied in multiple fields such as environmental remediation, intestinal microecological regulation, and the high-activity organic selenium industry chain.

[0073] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above description is illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A stress-resistant selenium-based quantum editing ecological regulation system, characterized in that, include: Quantum entanglement preparation module, quantum state acquisition and analysis unit, and artificial intelligence early warning and control module; The system uses an organic selenium functional chip as its core hardware carrier, which integrates selenium quantum dot sensing and gene editing functions. The quantum entanglement preparation module, the quantum state acquisition and analysis unit, and the artificial intelligence early warning and control module are electrically connected and interact with signals through the internal microcircuits and microfluidic channels of the chip. The organic selenium functional chip is equipped with a selenium quantum dot array sensing unit, a gene editing execution unit, and a signal feedback unit.

2. The stress-resistant selenium-based quantum editing ecological regulation system according to claim 1, characterized in that, The quantum entanglement preparation module includes a 532nm laser, a 0.1T magnetic field generator, a microfluidic sample unit, and a temperature control subunit.

3. The stress-resistant selenium-based quantum editing ecological regulation system according to claim 1, characterized in that, The quantum state acquisition and analysis unit incorporates a quantum state analyzer, a quantum-microbial community collaborative decoding algorithm chip, and a data preprocessing module.

4. The resilient selenium-based quantum editing ecological regulation system according to claim 1, characterized in that, The artificial intelligence early warning and control module includes a machine learning early warning chip, an intervention command generation unit, and a nanocarrier targeted delivery control interface.

5. The resilient selenium-based quantum editing ecological regulation system according to claim 1, characterized in that, The selenium quantum dot array sensing unit is fixed to the chip substrate via EDC / NHS chemical coupling. The coupling reaction pH is 6.0-7.0, and the selenium quantum dot surface is coated with a PVP coating with a thickness of 2-5 nm.

6. The resilient selenium-based quantum editing ecological regulation system according to claim 1, characterized in that, The gene editing execution unit has a CRISPR-Cas9 protein component fixed in situ, and the gene editing execution unit is configured to adjust the activity of the Cas9 protein and the targeting of the editing site based on quantum signals.

7. The system according to any one of claims 1-6 is applied in environmental remediation, intestinal microecological regulation, and the high-activity organic selenium industry chain.

8. A method for constructing a stress-resistant selenium-based quantum editing ecological regulation system, characterized in that, Includes the following steps: S1. Gene editing of key genes in the selenium metabolism pathway of engineered bacteria based on an organic selenium functional chip; S2. Microbial samples and gene-edited engineered bacteria are placed in a magnetic field integrated with a chip and irradiated with a laser to prepare quantum entangled pairs in situ on an organoselenium functional chip. S3. Collect quantum signals through the quantum sensing module of the organic selenium functional chip and analyze the signals into bacterial community synergy efficiency parameters; S4. When the bacterial community synergy efficiency parameter is lower than the preset threshold, the chip drives the nanocarrier to deliver the organic selenium activator in a targeted manner.

9. The method for constructing a stress-resistant selenium-based quantum editing ecological regulation system according to claim 8, characterized in that, The gene editing described in step S1 involves knocking out the recA gene of engineered bacteria using CRISPR technology. The gene editing process is tracked in real time by a selenium quantum dot fluorescent probe on the chip. The magnetic field strength used in the quantum entanglement preparation process is 0.1T, and the laser wavelength is 532nm. The system automatically performs closed-loop control based on the collected quantum signals.

10. The method according to any one of claims 8-9 is applied in environmental remediation, intestinal microecological regulation, and the high-activity organic selenium industry chain.