A cell culture chamber capable of gradient oxygen supply

Abstract

The application discloses a cell culture chamber capable of gradient oxygen supply and belongs to the technical field of cell culture. The cell culture chamber comprises a cover box, culture slides and a separation bin. A feeding opening is formed in one side of the cover box. Connecting strips are fixedly connected to the inner walls of the two sides of the cover box. The cover box is slidably connected to the separation bin through the connecting strips. A driving motor is fixedly installed on the side wall of the cover box. In the application, the connecting shaft is driven to rotate by the gear. In the process, the connecting shaft drives the through block at the end and the I-shaped plate to synchronously rotate, so that the sliding block slides along the runway-shaped track of the guide groove. In the sliding process, the sliding block is pushed by the guide groove wall, the I-shaped plate can slide through the through block, and the detection positions of the oxygen sensors in the main chamber, the auxiliary chamber and the secondary chamber are changed. In this way, the oxygen sensor reciprocating scanning is realized, the limitation of traditional fixed-point detection is broken, the oxygen concentration data of different regions are collected, and the detection blind area is avoided.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, and in particular relates to a cell culture chamber with gradient oxygen supply. Background Technology

[0002] Cell culture chambers are a general term for devices in the laboratory that provide a controlled in vitro growth environment for cells. In a broad sense, they include micro-segmented chambers in CO2 incubators, Transwell chambers, chamber slides, etc. In addition, they also include multilayer cell factories, roller bottle culture systems, microfluidic chip chambers, and modular chambers in bioreactors.

[0003] Traditional culture chambers mostly use a fixed-point sampling mode, with sensors installed in fixed positions. This can only obtain local environmental parameters at the detection point, making it difficult to achieve full-area detection of the chamber. Due to factors such as gas diffusion effects and local consumption, the actual oxygen partial pressure in different areas of the chamber varies spatially. Data obtained from single-point acquisition has obvious limitations. Therefore, there is an urgent need for a cell culture chamber with gradient oxygen supply to solve the above problems. Summary of the Invention

[0004] The purpose of this invention is to address the problem that traditional culture chambers, which use fixed-point sampling and fixed sensor positions, can only acquire local environmental parameters and cannot reflect the spatial distribution differences of oxygen partial pressure throughout the chamber, resulting in significant limitations in single-point data acquisition. Therefore, this invention proposes a cell culture chamber with gradient oxygen supply.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A cell culture chamber with gradient oxygen supply includes a cover box, culture slides, and a separator. The cover box has a feed port on one side, and connecting strips are fixedly connected to the inner walls on both sides of the cover box. The cover box is slidably connected to the separator via the connecting strips. A drive motor is fixedly installed on one side wall of the cover box. The output shaft of the drive motor is connected to a light rod. A sliding sleeve is slidably connected through the smooth middle section of the light rod, and threaded sleeves are slidably installed through both ends of the light rod.

[0007] Connecting plates are fixedly connected to both sides of the threaded sleeve. A push rod is fixedly connected to one side of the connecting plate. A fixing plate is fixedly connected to one end of both connecting plates. An insert plate is fixedly connected below the fixing plate. Vertical plates are fixedly connected to both sides of the light rod above the partition compartment. A main chamber, a secondary chamber, and a secondary chamber are equally spaced below the partition compartment. Guide grooves are opened on the inner walls above the main chamber, the secondary chamber, and the secondary chamber, and the guide groove trajectory is designed in a racetrack shape.

[0008] The main chamber, auxiliary chamber, and secondary chamber are located at opposite ends of the diagonal and are respectively fixedly connected to an air inlet pipe and an exhaust pipe. Both the air inlet pipe and the exhaust pipe pass through the upper wall of the cover and are slidably connected to the cover. The main chamber is fixedly connected to a main connecting pipe through the air inlet pipe, the auxiliary chamber is fixedly connected to a secondary connecting pipe through the air inlet pipe, and the secondary chamber is fixedly connected to a secondary connecting pipe through the air inlet pipe. The upper walls of the main chamber, auxiliary chamber, and secondary chamber are rotatably connected to a connecting shaft through bearings.

[0009] A gear is fixedly installed on the connecting shaft. A through block is fixedly connected to one end of the connecting shaft. An I-shaped plate is slidably connected through the through block. A slider is fixedly connected above one end of the I-shaped plate. The slider and the guide groove are both T-shaped and the slider slides in the guide groove. An oxygen sensor is fixedly installed below the slider on the I-shaped plate. Horizontal grooves are opened on both sides of the upright plate, and a sloping top groove is opened at one end of the upright plate located in the horizontal groove.

[0010] The upright plate is provided with a guide groove between the two inclined top grooves. A pushing mechanism is slidably installed in the guide groove. The pushing mechanism includes a main frame. Fixed plates are fixedly connected to both sides of the main frame. A secondary frame is fixedly connected to one end of each fixed plate on both sides of the main frame. A rack is fixedly connected to the inner wall of one side of the main frame and the secondary frame. A through plate is fixedly connected to one side of the upper part of the main frame.

[0011] As a further description of the above technical solution:

[0012] An electric push rod is fixedly installed on the inner wall above the cover box on one side of the sliding sleeve. The movable end of the electric push rod is fixedly connected to the sliding sleeve. A side plate is fixedly connected to one side of the sliding sleeve. A through groove is opened on the side plate. The through plate passes through the through groove and slides with the through groove.

[0013] As a further description of the above technical solution:

[0014] The culture slide is inserted into the feed inlet and has slots on both sides below it. Both the slots and the insert plate are designed as isosceles triangles. Three culture chambers are equally spaced above the culture slide. Rubber rings are fixedly connected around the culture chambers above the culture slide.

[0015] As a further description of the above technical solution:

[0016] The connecting plate, fixing plate and insert plate are U-shaped in design. The bottom two sides of the cover box have opening slots, and the upper part of the cover box has through holes on the walls on both sides of the light rod. The connecting plate slides through the through holes and is slidably connected to the through holes. The insert plate is inserted into the slot through the opening slot.

[0017] As a further description of the above technical solution:

[0018] Both sides of the partition compartment are provided with connecting grooves, and the connecting grooves and connecting strips are T-shaped. The connecting strips and connecting grooves are slidably engaged. The partition compartment is provided with fitting grooves around the main chamber, auxiliary chamber and secondary chamber below, and the rubber ring is fitted into the fitting grooves.

[0019] As a further description of the above technical solution:

[0020] The light rod is rotatably connected to the cover box via a bearing. The two ends of the light rod are provided with threads in opposite directions and are threaded together with the threaded sleeve. The connecting plate is slidably disposed between the two vertical plates and abuts against the wall of the vertical plates. The push rod slides in the transverse groove and the inclined top groove.

[0021] As a further description of the above technical solution:

[0022] The fixed plate slides into the guide groove, the positions of the main frame and the two sub-frames correspond to the positions of the main chamber, sub-chamber, and secondary chamber, and the rack meshes with the gear.

[0023] As a further description of the above technical solution:

[0024] A filter mechanism is inserted into the end of the exhaust pipe. The filter mechanism includes a plug sleeve, which is inserted into the end of the exhaust pipe, and a hydrophobic filter membrane is fixedly disposed below the plug sleeve.

[0025] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are: 1. In this invention, gradient-proportioned oxygen mixtures are supplied to the main chamber, secondary chamber, and sub-chamber respectively through the main connecting pipe, auxiliary connecting pipe, and secondary connecting pipe, constructing three standard oxygen gradient culture environments: high oxygen, normal oxygen, and low oxygen. During the culture and oxygen supply process, the sliding sleeve is pushed back and forth along the smooth section of the smooth rod by activating the electric push rod. During this process, the sliding sleeve is synchronously pulled by the side plate to move the entire push mechanism laterally, causing the fixed plate to slide along the guide groove. This allows the main frame and the two side sub-frames to move laterally synchronously, enabling the racks on the inner sides of the main frame and sub-frames to separate. The gear at the corresponding position of the non-meshing push is caused to rotate the connecting shaft. During the process, the connecting shaft drives the end block and the I-shaped plate to rotate synchronously, causing the slider to slide along the racetrack-shaped trajectory of the guide groove. During the sliding process, the slider is pushed by the guide groove wall, which allows the I-shaped plate to slide through the through block, thereby changing the detection position of each oxygen sensor in the main chamber, auxiliary chamber, and secondary chamber. This enables the oxygen sensor to reciprocate and scan, breaking the limitations of traditional fixed-point detection, completing the collection of oxygen concentration data in different areas, and avoiding detection blind spots. 2. In this invention, by starting the drive motor, the drive motor drives the optical rod to rotate. During this process, the optical rod drives the two threaded sleeves to move synchronously towards each other, causing the threaded sleeves to drive the connecting plate through the through hole, thereby causing the fixing plate and the insert plate to move horizontally as a whole. This allows the insert plate to pass through the opening slot and be inserted into the slot below the culture slide. As the connecting plate moves, the push rod on one side of the connecting plate can gradually slide from the horizontal slot into the inclined top slot, thereby causing the push rod to push against the inclined wall of the inclined top slot, causing the vertical plate to drive the entire partition chamber along the connecting strip. The slide moves vertically downwards, allowing multiple air inlet and exhaust pipes to slide across the top of the enclosure. As the compartments continue to descend, the rubber rings on the culture slides can be embedded into the fitting grooves. The main chamber, secondary chamber, and sub-chamber then cover the three culture chambers, thus achieving the positioning and locking of the culture slides and the synchronous sealing of multiple culture chambers. During the insertion of the insert plate into the slot, the isosceles triangular inclined planes of the insert plate and slot can be used to push up the culture slides during the insertion process, correcting the position of the culture slides. Attached Figure Description

[0026] Figure 1 This is a three-dimensional structural diagram of a cell culture chamber with gradient oxygen supply proposed in this invention;

[0027] Figure 2 This is a schematic cross-sectional view of the cover structure of a cell culture chamber with gradient oxygen supply proposed in this invention.

[0028] Figure 3 This is a schematic cross-sectional view of the partition structure of a cell culture chamber with gradient oxygen supply proposed in this invention.

[0029] Figure 4 This is a schematic cross-sectional view of the exhaust pipe structure of a cell culture chamber with gradient oxygen supply proposed in this invention.

[0030] Figure 5 This is a schematic diagram of the three-dimensional structure of a vertical plate in a cell culture chamber with gradient oxygen supply proposed in this invention.

[0031] Figure 6 This is a schematic diagram of the three-dimensional structure of a cell culture chamber with gradient oxygen supply proposed in this invention;

[0032] Figure 7 This is a three-dimensional structural diagram of a pusher mechanism for a cell culture chamber with gradient oxygen supply proposed in this invention.

[0033] Figure 8 This is a schematic diagram of the three-dimensional structure of a cell culture chamber with gradient oxygen supply proposed in this invention.

[0034] Legend: 1. Cover box; 101. Inlet; 102. Connecting strip; 103. Drive motor; 104. Smooth rod; 105. Sliding sleeve; 106. Threaded sleeve; 107. Connecting plate; 108. Push rod; 109. Fixing plate; 110. Insert plate; 111. Side plate; 112. Through slot; 113. Opening slot; 114. Perforation; 2. Culture slide; 201. Slot; 202. Culture chamber; 203. Rubber ring; 3. Separator; 301. Vertical plate; 302. Main chamber; 303. Secondary chamber; 304. Secondary chamber; 305. Guide groove; 306. Air inlet pipe; 307. 308. Exhaust pipe; 309. Main connecting pipe; 310. Secondary connecting pipe; 311. Connecting shaft; 312. Gear; 313. Through block; 314. I-shaped plate; 315. Slider; 316. Oxygen sensor; 317. Transverse groove; 318. Inclined top groove; 319. Guide groove; 320. Connecting groove; 321. Fitting groove; 4. Pushing mechanism; 401. Main frame; 402. Fixed plate; 403. Sub-frame; 404. Rack; 405. Through plate; 5. Electric push rod; 6. Filtering mechanism; 601. Insert sleeve; 602. Hydrophobic filter membrane. Detailed Implementation

[0035] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] In its specific implementation, such as Figures 1-8 The present invention provides a technical solution:

[0037] A cell culture chamber with gradient oxygen supply includes a cover box 1, culture slides 2, and partition chambers 3. A feed inlet 101 is located on one side of the cover box 1. Connecting strips 102 are fixedly connected to the inner walls of both sides of the cover box 1. The cover box 1 is slidably connected to the partition chambers 3 via the connecting strips 102. The culture slides 2 are inserted into the feed inlet 101 and have slots 201 on both sides below them. Both the slots 201 and the insert plates 110 are designed in an isosceles triangle shape. Three culture chambers 202 are evenly spaced above the culture slides 2. A rubber ring 203 is fixedly connected above the culture slide 2 around the culture chamber 202. The rubber ring 203 is inserted into the fitting groove 321. Through the insertion and fitting of the rubber ring 203 and the fitting groove 321, the deformation of the rubber ring 203 fills the gap between the fitting groove 321 and the culture slide 2, so that the separator 3 can form a seal when it comes into contact with the culture slide 2, thereby preventing cross-leakage of gas between the main chamber 302, the secondary chamber 303 and the secondary chamber 304.

[0038] A drive motor 103 is fixedly installed on one side wall of the cover box 1. The output shaft of the drive motor 103 is connected to a guide rod 104. The guide rod 104 is rotatably connected to the cover box 1 via a bearing. A sliding sleeve 105 is slidably connected through the smooth section in the middle of the guide rod 104. An electric push rod 5 is fixedly installed on the upper inner wall of the cover box 1, located on one side of the sliding sleeve 105. The movable end of the electric push rod 5 is fixedly connected to the sliding sleeve 105. A side plate 111 is fixedly connected to one side of the sliding sleeve 105. A through groove 112 is provided on the side plate 111. Threaded sleeves 106 are slidably installed through both ends of the guide rod 104. The two ends of the guide rod 104 are provided with threads in opposite directions that are threadedly engaged with the threaded sleeves 106. Connecting plates 107 are fixedly connected to both sides of the threaded sleeves 106. A push rod 108 is fixedly connected to one side of the connecting plates 107. The two connecting plates 107 share a common feature at one end. A fixing plate 109 is fixedly connected to the cover 1, and an insert plate 110 is fixedly connected below the fixing plate 109. The connecting plate 107, the fixing plate 109, and the insert plate 110 are U-shaped in design. Opening slots 113 are provided on both sides of the bottom of the cover 1, and through holes 114 are provided on the walls on both sides of the light rod 104 above the cover 1. The connecting plate 107 slides through the through holes 114 and is slidably connected to the through holes 114. The insert plate 110 is inserted into the slot 201 through the opening slots 113. Through the sliding connection between the connecting plate 107 and the through holes 114, the connecting plate 107 can form a guide and support when it moves horizontally with the threaded sleeve 106, so as to prevent the connecting plate 107 from swaying during the movement and ensure that the fixing plate 109 and the insert plate 110 can smoothly pass through the opening slots 113 and be inserted into the slot 201 below the culture slide 2.

[0039] Above the partition compartment 3, upright plates 301 are fixedly connected to both sides of the light rod 104. Below the partition compartment 3, a main chamber 302, a secondary chamber 303, and a secondary chamber 304 are arranged at equal intervals. Connecting grooves 320 are provided on both sides of the partition compartment 3, and the connecting grooves 320 and the connecting strips 102 are all T-shaped. The connecting strips 102 and the connecting grooves 320 slide together. The partition compartment 3 is surrounded by fitting grooves 321 below the main chamber 302, the secondary chamber 303, and the secondary chamber 304. Through the T-shaped design of the connecting strips 102 and the connecting grooves 320, the connecting strips 102 can guide the partition compartment 3 during the up and down movement of the partition compartment 3, thereby ensuring the stability of the movement of the partition compartment 3 and preventing the partition compartment 3 from tilting during the lifting and lowering process.

[0040] Guide grooves 305 are provided on the inner walls of the main chamber 302, the auxiliary chamber 303, and the secondary chamber 304, and the guide grooves 305 are designed in a racetrack shape. The main chamber 302, the auxiliary chamber 303, and the secondary chamber 304 are fixedly connected to the air inlet pipe 306 and the exhaust pipe 307 at opposite ends of the diagonal. The air inlet pipe 306 and the exhaust pipe 307 both pass through the upper wall of the cover box 1 and are slidably connected to the cover box 1. A filter mechanism 6 is inserted into the end of the exhaust pipe 307. The filter mechanism 6 includes a plug sleeve 601, which is inserted into the end of the exhaust pipe 307. A hydrophobic filter membrane 602 is fixedly provided below the plug sleeve 601. By inserting the plug sleeve 601 into the end of the exhaust pipe 307, the hydrophobic filter membrane 602 is sealed at the exhaust port of the exhaust pipe 307. By utilizing the air permeability and water resistance characteristics of the hydrophobic filter membrane 602, impurities are prevented from entering while allowing the gas to be discharged normally.

[0041] The main chamber 302 is fixedly connected to a main connecting pipe 308 via an air inlet pipe 306. The auxiliary chamber 303 is fixedly connected to an auxiliary connecting pipe 309 via an air inlet pipe 306. The secondary chamber 304 is fixedly connected to a secondary connecting pipe 310 via an air inlet pipe 306. The upper walls of the main chamber 302, auxiliary chamber 303, and secondary chamber 304 are rotatably connected to a connecting shaft 311 via bearings. A gear 312 is fixedly installed on the connecting shaft 311. A through block 313 is fixedly connected to one end of the connecting shaft 311. An I-shaped plate 314 slides through the through block 313. A slider 315 is fixedly connected above one end of the I-shaped plate 314. Both the slider 315 and the guide groove 305 are T-shaped, and the slider 315 slides within the guide groove 305. The I-shaped plate 314 is located above the slider 315. An oxygen sensor 316 is fixedly installed below the 15. Horizontal grooves 317 are provided on both sides of the upright plate 301, and an inclined top groove 318 is provided at one end of the horizontal groove 317. The connecting plate 107 is slidably disposed between the two upright plates 301 and abuts against the wall of the upright plate 301. The push rod 108 slides within the horizontal groove 317 and the inclined top groove 318. Through the T-shaped design of the slider 315 and the guide groove 305, the slider 315 can be guided and limited when sliding within the guide groove 305, effectively preventing the slider 315 from derailing in the racetrack-shaped trajectory and maintaining the stability of the I-shaped plate 314. The abutment between the two upright plates 301 and the wall of the connecting plate 107 can restrict the connecting plate 107 and prevent it from twisting during movement.

[0042] A guide groove 319 is provided between two inclined top grooves 318 on the upright plate 301. A pushing mechanism 4 is slidably installed in the guide groove 319. The pushing mechanism 4 includes a main frame 401. Fixed plates 402 are fixedly connected to both sides of the main frame 401. The fixed plates 402 are slidably engaged with the guide groove 319. A secondary frame 403 is fixedly connected to one end of each fixed plate 402 on both sides of the main frame 401. A rack 404 is fixedly connected to the inner wall of one side of the main frame 401 and the secondary frame 403. The positions of the main frame 401 and the two secondary frames 403 correspond to the positions of the main chamber 302, the secondary chamber 303, and the secondary chamber 304. The rack 404 meshes with the gear 312. A through plate 405 is fixedly connected to one side of the upper part. The through plate 405 slides through the through groove 112 and is slidably engaged with the through groove 112. Through the sliding engagement of the fixed plate 402 and the guide groove 319, the guide groove 319 can guide the overall movement of the pushing mechanism 4 and prevent the movement from deviating. The sliding engagement of the through plate 405 and the through groove 112 allows the through plate 405 to slide vertically in the through groove 112 when the pushing mechanism 4 moves down with the partition compartment 3. This provides guidance and support for the lifting and translation of the main frame 401 and the two side sub-frames 403, and forms a linkage traction for the subsequent movement of the sliding sleeve 105, so that the sliding sleeve 105 can drive the overall lateral movement of the pushing mechanism 4.

[0043] Working principle: After the culture slide 2 is cultured, the culture slide 2 is pushed into the cover box 1 from the feed port 101 until it abuts against the inner wall of the cover box 1. Then the drive motor 103 is started, which drives the light rod 104 to rotate. During the process, the light rod 104 drives the two threaded sleeves 106 to move towards each other synchronously by relying on the reverse threads at both ends. The threaded sleeves 106 drive the connecting plate 107 to pass through the through hole 114, which in turn drives the fixing plate 109 and the insert plate 110 to move horizontally as a whole. The insert plate 110 passes through the opening slot 113 and is inserted into the slot 201 below the culture slide 2. The isosceles triangular inclined plane of the insert plate 110 and the slot 201 is utilized. This allows the culture slide 2 to be pushed up during the insertion process, correcting its position. As the connecting plate 107 moves, the push rod 108 on one side of the connecting plate 107 can gradually slide from the transverse groove 317 into the inclined groove 318, thereby pushing the push rod 108 against the inclined groove wall of the inclined groove 318. This causes the vertical plate 301 to drive the partition chamber 3 to move vertically downward along the connecting strip 102. During this process, the vertical plate 301 simultaneously drives the push mechanism 4 to move downward, allowing the through plate 405 to slide vertically down in the through groove 112. Meanwhile, multiple air inlet pipes 306 and exhaust pipes 307 slide and pass through the cover box 1. As the partition chamber 3 moves downward, the rubber ring 203 on the culture slide 2 can be embedded into the fitting groove 321, while the main chamber 302, the secondary chamber 303, and the secondary chamber 304 cover the three culture chambers 202 respectively, thereby completing the positioning and locking of the culture slide 2 and the synchronous sealing of the multiple culture chambers 202. Then, gas supply devices with different oxygen concentrations are connected to the main connecting pipe 308, the secondary connecting pipe 309, and the secondary connecting pipe 310 respectively. Gradiently proportioned oxygen mixed gas is delivered to the main chamber 302, the secondary chamber 303, and the secondary chamber 304 through the main connecting pipe 308, the secondary connecting pipe 309, and the secondary connecting pipe 310 respectively, to construct three sets of standard oxygen gradient culture environments: high oxygen, normal oxygen, and low oxygen. During the cultivation and oxygen supply process, by activating the electric push rod 5, the electric push rod 5 is prompted to reciprocate to push the sliding sleeve 105 along the smooth section of the smooth rod 104. During this process, the sliding sleeve 105 drives the side plate 111 to move laterally, and the side plate 111 pulls the entire push mechanism 4 through the sliding cooperation of the through groove 112 and the through plate 405, so that the fixed plate 402 slides along the guide groove 319, thereby enabling the main frame 401 and the two side sub-frames 403 to move laterally synchronously, so that the racks 404 on the inner side of the main frame 401 and the sub-frames 403 respectively mesh with the gears 312 at the corresponding push positions, causing the gears 312 to drive the connecting shaft 311 to rotate. During the process, the connecting shaft 311 drives the end through block 313 and I-shaped plate 314 to rotate synchronously. The rotating I-shaped plate 314 can drive the slider 315 and oxygen sensor 316, causing the slider 315 to slide along the racetrack-shaped trajectory of the guide groove 305. During the sliding process, the slider 315 is pushed by the groove wall of the guide groove 305, pushing and driving the I-shaped plate 314 to slide through the through block 313, thereby changing the detection position of each oxygen sensor 316 in the main chamber 302, auxiliary chamber 303 and secondary chamber 304, so as to realize the collection of oxygen concentration data in different areas.

Claims

1. A cell culture chamber with gradient oxygen supply, characterized in that, include: The enclosure (1), culture slides (2), and separator (3) are provided. A material inlet (101) is provided on one side of the enclosure (1). Connecting strips (102) are fixedly connected to the inner walls on both sides of the enclosure (1). The enclosure (1) is slidably connected to the separator (3) through the connecting strips (102). A drive motor (103) is fixedly installed on one side wall of the enclosure (1). A light rod (104) is connected to the output shaft of the drive motor (103). A sliding sleeve (105) is slidably connected through the smooth section in the middle of the light rod (104). Threaded sleeves (106) are slidably installed through both ends of the light rod (104). The threaded sleeve (106) is fixedly connected to two sides by connecting plates (107), and a push rod (108) is fixedly connected to one side of the connecting plate (107). A fixing plate (109) is fixedly connected to one end of the two connecting plates (107). An insert plate (110) is fixedly connected below the fixing plate (109). A vertical plate (301) is fixedly connected to both sides of the light rod (104) above the partition chamber (3). A main chamber (302), a secondary chamber (303), and a secondary chamber (304) are equally spaced below the partition chamber (3). A guide groove (305) is opened on the inner wall above the main chamber (302), the secondary chamber (303), and the secondary chamber (304), and the trajectory of the guide groove (305) is designed in the shape of a racetrack. The main chamber (302), the auxiliary chamber (303), and the secondary chamber (304) are located at opposite ends of the diagonal and are respectively fixedly connected to an air inlet pipe (306) and an exhaust pipe (307). The air inlet pipe (306) and the exhaust pipe (307) both pass through the upper wall of the cover (1) and are slidably connected to the cover (1). The main chamber (302) is fixedly connected to a main connecting pipe (308) through the air inlet pipe (306). The auxiliary chamber (303) is fixedly connected to a secondary connecting pipe (309) through the air inlet pipe (306). The secondary chamber (304) is fixedly connected to a secondary connecting pipe (310) through the air inlet pipe (306). The upper walls of the main chamber (302), the auxiliary chamber (303), and the secondary chamber (304) are rotatably connected to a connecting shaft (311) through a bearing. A gear (312) is fixedly installed on the connecting shaft (311). A through block (313) is fixedly connected to one end of the connecting shaft (311). An I-shaped plate (314) is slidably connected through the through block (313). A slider (315) is fixedly connected above one end of the I-shaped plate (314). The slider (315) and the guide groove (305) are both T-shaped, and the slider (315) slides in the guide groove (305). An oxygen sensor (316) is fixedly installed below the slider (315) on the I-shaped plate (314). A transverse groove (317) is opened on both sides of the upright plate (301), and a sloping top groove (318) is opened at one end of the upright plate (301) located in the transverse groove (317). The upright plate (301) is provided with a guide groove (319) between two inclined top grooves (318). A pushing mechanism (4) is slidably installed in the guide groove (319). The pushing mechanism (4) includes a main frame (401). Fixed plates (402) are fixedly connected to both sides of the main frame (401). A sub-frame (403) is fixedly connected to one end of each fixed plate (402) on both sides of the main frame (401). A rack (404) is fixedly connected to the inner wall of one side of the main frame (401) and the sub-frame (403). A through plate (405) is fixedly connected to one side above the main frame (401).

2. The cell culture chamber with gradient oxygen supply according to claim 1, characterized in that, An electric push rod (5) is fixedly installed on the inner wall above the cover box (1) on one side of the sliding sleeve (105). The movable end of the electric push rod (5) is fixedly connected to the sliding sleeve (105). A side plate (111) is fixedly connected to one side of the sliding sleeve (105). A through groove (112) is provided on the side plate (111). The through plate (405) passes through the through groove (112) and slides in cooperation with the through groove (112).

3. The cell culture chamber with gradient oxygen supply according to claim 1, characterized in that, The culture slide (2) is inserted into the feed inlet (101) and slots (201) are provided on both sides below. The slots (201) and the insert plate (110) are both designed as isosceles triangles. Three culture chambers (202) are provided at equal intervals above the culture slide (2). Rubber rings (203) are fixedly connected around the culture chambers (202) above the culture slide (2).

4. The cell culture chamber with gradient oxygen supply according to claim 3, characterized in that, The connecting plate (107), fixing plate (109) and insert plate (110) are U-shaped in design. The cover box (1) has opening slots (113) on both sides below. The cover box (1) has through holes (114) on the walls on both sides of the light rod (104) above. The connecting plate (107) slides through the through hole (114) and is connected to the through hole (114). The insert plate (110) is inserted into the slot (201) through the opening slot (113).

5. A cell culture chamber with gradient oxygen supply according to claim 3, characterized in that, The partition compartment (3) has connecting grooves (320) on both sides, and the connecting grooves (320) and the connecting strips (102) are T-shaped. The connecting strips (102) and the connecting grooves (320) slide together. The partition compartment (3) has fitting grooves (321) around the main chamber (302), the secondary chamber (303) and the secondary chamber (304) below it. The rubber ring (203) is fitted into the fitting grooves (321).

6. A cell culture chamber with gradient oxygen supply according to claim 1, characterized in that, The light rod (104) is rotatably connected to the cover box (1) through a bearing. The two ends of the light rod (104) are provided with threads in opposite directions and are threadedly engaged with the threaded sleeve (106). The connecting plate (107) is slidably disposed between the two vertical plates (301) and abuts against the wall of the vertical plate (301). The push rod (108) slides in the transverse groove (317) and the inclined top groove (318).

7. A cell culture chamber with gradient oxygen supply according to claim 1, characterized in that, The fixed plate (402) is slidably engaged with the guide groove (319), the positions of the main frame (401) and the two sub-frames (403) correspond to the positions of the main chamber (302), the sub-chamber (303) and the secondary chamber (304), and the rack (404) meshes with the gear (312).

8. A cell culture chamber with gradient oxygen supply according to claim 1, characterized in that, A filter mechanism (6) is inserted into the end of the exhaust pipe (307). The filter mechanism (6) includes a plug sleeve (601), which is inserted into the end of the exhaust pipe (307). A hydrophobic filter membrane (602) is fixedly disposed below the plug sleeve (601).

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