Emersonia zhangjianhua ZDC-1 and microalgae biofertilizer thereof and application thereof in saline-alkali soil

Abstract

The application discloses Emiliana ZDC-1 and a microalgae biofertilizer thereof and application of the microalgae biofertilizer in saline-alkali soil, and belongs to the technical field of microorganisms and fertilizers. The Emiliana ZDC-1 has a preservation number of CGMCC No. 46972, can secrete high levels of indole acetic acid (IAA, 4.50 μg·g ‑1 ) and soluble protein (425.72 mg·g ‑1 ). The microalgae biofertilizer comprises Emiliana ZDC-1 and a special carrier liquid, and the algal cell concentration is 1×10 7 ~1×10 8 cells·mL ‑1 . Under the condition of saline-alkali soil, the microalgae biofertilizer is applied in a mode of root dipping combined with root irrigation, can significantly promote the growth of peppers, improve the yield and fruit quality, can effectively reduce the soil pH, conductivity and total salt content, increase the organic matter and alkali-hydrolyzable nitrogen content, and has multiple effects of increasing the yield and improving the quality of peppers in saline-alkali soil and improving saline-alkali soil.

Description

Technical Field

[0001] This invention belongs to the field of microbial and fertilizer technology, specifically relating to Emerson Lipcoglobulinus (… Graesiella emersonii ZDC-1 and its microalgae bio-fertilizer and their application in chili pepper cultivation in saline-alkali land. Background Technology

[0002] Saline-alkali land refers to soils containing excessive soluble salts or a high proportion of exchangeable sodium ions. Saline-alkali land generally suffers from poor soil structure, slow water infiltration rates, low desalination efficiency, long treatment cycles, and a tendency for recurrence, severely limiting agricultural production. Saline-alkali land is widely distributed in my country, mainly in four major regions: the Northwest inland, the Songnen Plain in Northeast China, the Huang-Huai-Hai Plain, and the eastern coastal areas. Among them, the pH value of saline-alkali land in the eastern coastal areas is relatively high. At the same time, saline-alkali land with agricultural potential in my country is an important reserve of arable land. Therefore, the rational development and improvement of saline-alkali land resources is of great strategic significance for ensuring my country's food security and promoting sustainable agricultural development.

[0003] chili( Capsicum annuum Chili peppers are one of my country's major vegetable crops, possessing high nutritional and economic value. However, they are highly sensitive to salt-alkali stress. Studies have shown that under salt-alkali stress, chili pepper plants exhibit physiological disorders such as stunted growth, leaf yellowing and necrosis, and decreased root vitality, leading to reduced biomass accumulation, decreased fruit yield per plant, and ultimately, a significant reduction in both yield and quality. Particularly in arid regions of Northwest China and coastal saline-alkali areas, soil salinization has become a major limiting factor for the development of the chili pepper industry. Currently, production mainly relies on measures such as breeding salt-alkali tolerant varieties, rational irrigation, and mulching cultivation to alleviate salt-alkali stress, but their effectiveness remains limited.

[0004] To address the challenges of crop cultivation in saline-alkali land, various saline-alkali land improvement technologies have been developed both domestically and internationally. These technologies can be broadly categorized into four types: engineering measures, agronomic measures, chemical measures, and biological measures. Engineering measures mainly include constructing irrigation and drainage systems, underground salt drainage, and land leveling. These methods lower the groundwater level by leaching and removing soil salts, representing a fundamental approach to saline-alkali land management. However, engineering measures are costly, time-consuming, and face practical problems such as high water consumption and drainage difficulties in large-scale implementation. Chemical measures involve adding chemical amendments such as desulfurized gypsum, ferrous sulfate, and humic acid to the soil to reduce soil alkalinity through ion exchange reactions. However, chemical amendments suffer from problems such as large dosage requirements, potential secondary pollution, and unstable long-term effects. Biological measures include planting salt-tolerant plants, applying microbial agents, and organic materials. These measures offer advantages such as environmental friendliness and sustainability. However, existing biological amendments often have limited growth-promoting functions and unstable effects, making it difficult to meet the actual needs of agricultural production in saline-alkali land.

[0005] In recent years, microalgae have attracted extensive attention in the field of agriculture as a new type of biological resource. Some microalgae can secrete active substances such as plant hormones (e.g., indole acetic acid IAA), extracellular polysaccharides, and amino acids through metabolic activities, which can directly promote plant growth. At the same time, after being applied to soil, microalgae can increase the content of organic matter, improve soil structure, and increase nutrient availability, thus having the dual functions of biological fertilizer and soil conditioner. Previous studies have shown that salt-tolerant microalgae selected from extreme desert environments can be introduced into saline-alkali soil through spraying, thereby efficiently reducing soil salinity through ion adsorption and exchange functions, and improving nutrient availability and restructuring soil aggregate structure. However, the available salt-tolerant soil algal resources are still limited, and there is a lack of special microalgae biological fertilizer products and supporting application techniques for specific crops (e.g., peppers) planted in saline-alkali soil. SUMMARY

[0006] To overcome the deficiencies of the prior art, the present application provides a Chlorella emersonii ZDC-1 and a microalgae biological fertilizer thereof, which have the functions of improving saline-alkali soil and increasing the yield and quality of peppers. The microalgae has a preservation number of CGMCC No. 46972, and after being applied to saline-alkali soil, it can significantly promote the growth of peppers, increase the yield and quality of fruits, effectively reduce the soil pH, electrical conductivity, and total salt content, and increase the organic matter and alkali-hydrolyzable nitrogen content, thus having multiple functions of improving saline-alkali soil and increasing the yield and quality of peppers.

[0007] To achieve the above-mentioned purposes, the technical solutions adopted by the present application are as follows: Firstly, the present application provides a Chlorella emersonii ZDC-1, which was preserved in the China General Microbiological Culture Collection Center on June 24, 2026, has a preservation address of Beijing, China, and a preservation number of CGMCC No. 46972, and is classified and named as Chlorella emersonii. Graesiella emersonii .

[0008] Secondly, the present application provides a microbial culture containing the Chlorella emersonii ZDC-1.

[0009] Further, the culture is a fermentation broth of the Chlorella emersonii ZDC-1 or a concentrated liquid thereof.

[0010] Thirdly, the present application provides a microalgae biological fertilizer containing the Chlorella emersonii ZDC-1 and a special carrier liquid; the concentration of the Chlorella emersonii ZDC-1 in the microalgae biological fertilizer is 1×10 7 ~ 1×10 8 cells·mL -1 .

[0011] Further, the special carrier liquid contains the following components per liter: NaNO320~30 mg, CaCl2·2H2O 2~3 mg, MgSO4·7H2O 7~8 mg, K2HPO47~8 mg, KH2PO417~18 mg, NaCl 2~3 mg, Na2EDTA 4~6 mg, FeSO4·7H2O 0.4~0.6 mg, H3BO31~2 mg, MnCl2·4H2O 0.5~0.6 mg, ZnSO4·7H2O 0.85~0.95 mg, Na2MoO4·2H2O 0.07~0.09 mg, CuSO4·5H2O 0.14~0.18 mg, and Co(NO3)2·6H2O 0.04~0.06 mg.

[0012] Fourthly, the present application provides a method for preparing the microalgae bio-fertilizer, comprising the following steps: (1) culturing Emiliana huxleyi ZDC-1 and collecting wet algal sludge by centrifugation; (2) preparing the special carrier liquid in the amount; (3) mixing the wet algal sludge collected in step (1) with the special carrier liquid in step (2) and setting the volume to the concentration of Emiliana huxleyi ZDC-1 of 1×10 7 ~ 1×10 8 cells·mL -1 .

[0013] Further, the culturing in step (1) is carried out by using BG11 liquid medium, under the conditions of temperature 25~30℃, light intensity 2700±50 lx, light-dark cycle 14~18h:10~6h, and aeration until the late logarithmic growth phase.

[0014] Fifthly, the present application provides the application of Emiliana huxleyi ZDC-1, the microbial culture or the microalgae bio-fertilizer in promoting the growth of pepper, improving the yield of pepper and / or improving the saline-alkali soil.

[0015] Sixthly, the present application provides a method for using the microalgae bio-fertilizer, which is applied by combining the root dipping and root irrigation: before the transplantation of pepper seedlings, the root of the seedling is completely immersed in the microalgae bio-fertilizer, and the seedling is transplanted to the saline-alkali soil or the potting after soaking for 20~40 minutes.

[0016] Further, the root irrigation is applied once at the 7th, 14th and 21st day after the transplantation.

[0017] Further, the application amount per plant is 15~20 mL each time of root irrigation.

[0018] Compared with the prior art, the present application has the following beneficial effects: (1) The present application provides a multi-functional Emiliana huxleyi ZDC-1. The strain can secrete high levels of indole acetic acid (IAA, 4.50 μg·g -1 ) and soluble protein (425.72 mg·g -1 ), and can directly stimulate the root development of pepper and promote plant growth. Experiments show that after applying the microalgae biofertilizer of the present application in saline-alkali soil, the plant height, stem diameter, fresh weight and root activity of the pepper are significantly increased by 19.3%, 4.5%, 10.5% and 22.7%, respectively.

[0019] (2) The microalgae biofertilizer of the present application can significantly improve the yield and fruit quality of pepper in saline-alkali soil. Experiments show that after application, the yield of pepper is significantly increased by 10.0%, and the fruit length and width are increased by 11.0% and 13.6%, respectively; the soluble protein, soluble sugar, vitamin C content and color value of the fruit are significantly increased by 20.3%, 10.8%, 62.5% and 19.6%, respectively.

[0020] (3) The microalgae biofertilizer of the present application can effectively improve the physical and chemical properties of the soil. Experiments show that after application, the soil pH, electrical conductivity and total salt content are significantly decreased by 4.8%, 22.2% and 15.4%, respectively, and the organic matter and alkali-hydrolyzable nitrogen content are significantly increased by 29.8% and 13.2%, respectively, indicating that the microalgae biofertilizer has multiple functions of promoting crop growth, increasing yield and improving quality, and improving saline-alkali soil.

[0021] (4) The special carrier liquid used in the present application not only provides a basic ionic environment for Emiliana huxleyi ZDC-1 to maintain cell activity, but also avoids the secondary burden of exogenous nutrients on saline-alkali soil due to its low salt and low nutrient characteristics, and can synergistically play a role with the metabolic products of microalgae, which is beneficial to maintain the activity stability of the microalgae biofertilizer during storage and transportation.

[0022] (5) The present application adopts a combination of dipping roots and root irrigation. Dipping roots allows algal cells to densely colonize the root surface at the early stage of transplanting, rapidly exerting a growth-promoting effect; subsequent three times of root irrigation continuously supplements algal cells, enhancing the long-acting effect. Compared with simple root irrigation, the present application significantly improves the survival rate of pepper transplants. BRIEF DESCRIPTION OF DRAWINGS

[0023] The drawings accompanying the specification of the present application form a part thereof, serve to provide further understanding of the present application, and together with the description of the exemplary embodiments of the present application and their description serve to explain the present application, and do not constitute an improper limitation of the present application.

[0024] Figure 1Morphology, growth curve and phylogenetic tree of Emiliana huxleyi ZDC-1. a: plate morphology; b: micrograph; c: growth curve; d: phylogenetic tree based on 18S rDNA sequence, the branch number represents Bootstrap value; the lowermost scale is evolutionary distance; the accession number in the bracket is GenBank accession number of the algal strain.

[0025] Figure 2 Figure 1 is a comparison chart of the morphologies of pepper plants in saline-alkali soil after application of the microalgal biofertilizer of the present application.

[0026] Figure 3 Figure 2 is a comparison chart of the growth indicators of pepper plants in each treatment group, wherein (a) is plant height, (b) is stem diameter, (c) is fresh weight, (d) is total chlorophyll content, and (e) is root activity.

[0027] Figure 4 Figure 3 is a comparison chart of the morphologies of pepper fruits in each treatment group.

[0028] Figure 5 Figure 4 is a comparison chart of the yield and fruit size indicators of pepper fruits in each treatment group, wherein (a) is yield, (b) is fruit length, and (c) is fruit width.

[0029] Figure 6 Figure 5 is a comparison chart of the quality indicators of pepper fruits in each treatment group, wherein (a) is soluble protein content, (b) is soluble sugar content, (c) is vitamin C content, and (d) is color value.

[0030] Figure 7 Figure 6 is a comparison chart of the physicochemical properties of the soil for growing pepper plants in each treatment group, wherein (a) is pH, (b) is electrical conductivity, (c) is total salt content, (d) is organic matter content, and (e) is alkali-hydrolyzable nitrogen content. DETAILED DESCRIPTION

[0031] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the application. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0032] In order to enable those skilled in the art to more clearly understand the technical solutions of the present application, the technical solutions of the present application will be described in detail below with specific examples.

[0033] Example 1 Isolation, identification and property determination of Emiliana huxleyi ZDC-1 1. Isolation, identification and purification of soil microalgae Soil samples were collected from pepper plantations (36°3'38''N, 120°19'45''E) in Huangdao District, Qingdao City, Shandong Province, China, using the five-point mixed sampling method. A total of 500 g of soil samples were collected from 0-30 cm. The soil samples were mixed uniformly and then placed in sterile bags. The collected soil samples were mixed with BG11 liquid medium at a mass ratio of 1:2 in a 250 mL conical flask. The flask was incubated at 25±2 ℃ under light intensity of 3000±50 lux with a 12 h / 12 h light / dark cycle for 30 days. The enriched culture was spread on BG11 solid medium. The single algal colonies were obtained by multiple spread and streaking. Then, the single algal colonies were transferred to 20 mL of BG11 liquid medium for further culture. The purification operation was repeated three times to obtain a pure culture. The algal strain ZDC-1 formed green single algal colonies on the BG11 solid plate Figure 1 a).

[0034] 2. Morphological and molecular biological identification The morphology of microalgae was observed under an inverted microscope (Nikon ECLIPSE Ti2) and images were captured with NIS-Elements D 53000 64-bit software. As shown in Figure 1 b, the algal strain ZDC-1 was in the form of an oval or round single cell with a diameter of 6-10 μm, showing the morphological characteristics of E. sp. Graesiella emersonii .

[0035] The total DNA of the algal cells in the logarithmic growth phase was extracted using a plant tissue genomic DNA extraction kit. NS1 (5'-GTAGTCATATGCTTGTCTC-3', SEQ ID NO. 1) and NS4 (5'-CTTCCGTCAATTCCTTTAAG-3', SEQ ID NO. 2) were used as primers for PCR amplification. The PCR products were detected by 1% agarose gel electrophoresis and then sequenced by GenScript Biotech Corporation (Shanghai) Limited. The obtained sequence was subjected to BLAST comparison in the NCBI database. The results showed that the 18S rDNA sequence (SEQ ID NO. 3) of the algal strain ZDC-1 had 99.9% similarity with the 18S rDNA sequence (GenBank accession number OR333911.1) of the E. sp. strain NBR-NITD23. Graesiella emersonii Combining the morphological and molecular identification results, the algal strain was determined to be E. sp. Graesiella emersonii .

[0036] To further confirm its phylogenetic position, the MEGA 11 software was used to construct a phylogenetic tree by the neighbor-joining method. As shown in Figure 1 d, the algal strain ZDC-1 was closely related to E. sp. Graesiella emersoniiThe algal strains clustered within the same evolutionary branch, with 100% support, while they differed from other species in the same genus (such as...). Graesiella The sp. forms distinct branches. Based on morphological observation and molecular phylogenetic analysis, this algal strain was identified as *Emerersonia lipococcus*. Graesiella emersonii It was named ZDC-1.

[0037] The Emerson Lipnocarpus ZDC-1 described in this invention was deposited on June 24, 2026, at the China General Microbiological Culture Collection Center (CGMCC), Beijing, China, with accession number CGMCC No. 46972, and classified as follows: Graesiella emersonii .

[0038] 3. Growth curves, nutrient composition, and IAA secretion capacity of microalgae At an initial inoculation density of 1×10 5 cells·mL -1 Under the specified culture conditions, samples were taken every 48 hours, and the density of microalgae was determined using a hemocytometer until the plateau phase. Figure 1 As shown in Figure c, this algal strain entered the plateau phase on day 18, with a peak cell density of 3.0 × 10⁻⁶. 6 cells·mL -1 This indicates that the microalgae grows rapidly, has a short cultivation cycle, and possesses the potential for industrial production.

[0039] Microalgae in the plateau phase were collected, centrifuged, and freeze-dried. The soluble protein and total sugar contents were determined using a commercial kit from Beijing Solarbio Science & Technology Co., Ltd. Simultaneously, the IAA content of the microalgae was determined using the Salkowski colorimetric method described by Guardado-Fierros et al. (2024). The results are shown in Table 1.

[0040] Table 1. Soluble protein, total sugar and IAA content of Emerson Lipcospira ZDC-1

[0041] The above results indicate that the Emersonian Lipnocarpus ZDC-1 isolated in this invention not only possesses rapid growth characteristics but also secretes high levels of IAA (4.50 μg·g⁻¹). -1 ) and soluble protein (425.72 mg·g) -1 IAA, as an important plant growth hormone, can directly stimulate the development of pepper roots, promote the formation of lateral roots and root hairs, thereby enhancing the plant's ability to absorb soil nutrients. Its high content of soluble protein, once applied to the soil, can gradually decompose and release active nutrients such as amino acids, continuously supplying the pepper's growth. Therefore, this algal strain possesses excellent characteristics as a core strain for specialized bio-fertilizers.

[0042] Example 2 Preparation of Emiliana huxleyi ZDC-1 microalgae biofertilizer 1. Microalgae culture: Emiliana huxleyi ZDC-1 was inoculated into BG11 liquid medium and aerated at a temperature of 27℃, a light intensity of 2700±50 lx, and a light-dark cycle of 16h:8h. After the late logarithmic growth phase, the algal cell density was 1×10 7 ~ 1×10 8 cells·mL -1 . The culture solution was centrifuged at 5000 r·min -1 for 3 min, and the algal slurry was collected for use.

[0043] 2. Preparation of a special carrier solution: 25 mg of NaNO3, 2.5 mg of CaCl2·2H2O, 7.5 mg of MgSO4·7H2O, 7.5 mg of K2HPO4, 17.5 mg of KH2PO4, 2.5 mg of NaCl, 5.5 mg of Na2EDTA, 0.55 mg of FeSO4·7H2O, 1.14 mg of H3BO3, 0.55 mg of MnCl2·4H2O, 0.90 mg of ZnSO4·7H2O, 0.08 mg of Na2MoO4·2H2O, 0.16 mg of CuSO4·5H2O, and 0.05 mg of Co(NO3)2·6H2O were dissolved in 1L of distilled water, stirred uniformly, adjusted to pH 7.0 with NaOH or HCl, sterilized at 121℃ for 15 min, and cooled to room temperature to obtain the special carrier solution.

[0044] 3. Preparation of the microalgae biofertilizer: the wet algal slurry collected in step 1 was mixed with the special carrier solution prepared in step 2 to obtain a final concentration of Emiliana huxleyi ZDC-1 of about 1×10 7 cells·mL -1 , thereby obtaining the microalgae biofertilizer of the present application.

[0045] Example 3 Application effect of Emiliana huxleyi ZDC-1 microalgae biofertilizer on peppers 1. Test design The test was conducted in the test base of China Ocean University from May to September 2025. The pepper variety used was “Honglong 23”, and the saline-alkali soil used was taken from the coastal saline-alkali land in Huangdao District, Qingdao City, Shandong Province, with the following physicochemical properties: pH 8.40±0.05, electrical conductivity 1.81±0.05 ms·cm -1 , organic matter 14.02±0.09 g·kg -1 , and alkali-hydrolyzable nitrogen 46.56±3.34 mg·kg -1 .

[0046] Chili seeds were placed in a 50°C water bath for 15 minutes, and then soaked in sterile water at room temperature for 8 hours. They were then placed in a 27°C incubator for cultivation, maintaining a humid environment throughout the process. Once the seeds showed signs of sprouting, seeds with uniform sprouting length were selected and sown in seedling trays (the substrate was peat moss: perlite: vermiculite = 2:1:1).

[0047] The experiment included a control group (CK) and a microalgae biofertilizer treatment group ( G. emersonii Each group had 3 replicates, with each replicate containing 10 pots of chili pepper plants (2 plants per pot), for a total of 60 plants per group. Select chili pepper seedlings of uniform growth at the three-leaf-one-heart stage from the seedling trays and transplant them into flower pots (25 cm high, 23.5 cm in diameter), and designate the day of transplanting as day 1.

[0048] Microalgae bio-fertilizer treatment group: Before transplanting pepper seedlings, the roots of the seedlings were completely immersed in the microalgae bio-fertilizer prepared in Example 2 for 30 minutes, and then directly transplanted into flowerpots; on the 7th, 14th and 21st days after transplanting, 15 mL of the microalgae bio-fertilizer was applied to the soil around the roots of each seedling. A total of 4 applications were made.

[0049] Control group: The microalgae bio-fertilizer was replaced with an equal amount of sterile water, and the soaking and watering were carried out in the same way and for the same time. All other conditions were exactly the same as those of the experimental group.

[0050] Experimental data are expressed as mean ± standard deviation. Significance of differences between the treatment and control groups was assessed using one-way ANOVA (LSD test). P <0.05 indicates a significant difference.

[0051] 2. Determination of chili pepper growth indicators On the 45th day after transplanting, 10 pepper plants were randomly selected from each group. Plant height was measured using a measuring tape, stem diameter using calipers, and fresh weight using a balance. Total chlorophyll content in leaves was determined using the acetone-ethanol extraction method, and root activity was determined using the triphenyltetrazolium chloride (TTC) reduction method. Results are shown in Table 2 and... Figure 3 As shown.

[0052] Table 2 Effects of microalgae bio-fertilizer on chili pepper growth indicators

[0053] The results showed that, compared with the control group, the chili plants treated with the microalgae bio-fertilizer of this invention exhibited more vigorous growth and more developed stems and leaves. Figure 2 Compared with the control group, the application of the microalgae bio-fertilizer of this invention significantly increased the plant height, stem diameter, fresh weight, total chlorophyll, and root activity of chili peppers by 19.3%, 4.5%, 10.5%, 8.7%, and 22.7%, respectively.P <0.05; Figure 3 In a-e).

[0054] 3. Determination of pepper yield and fruit quality On the 125th day after transplanting, all the pepper fruits were picked up in each group, and the pepper fruit length and width were measured by a ruler, and the yield was determined. Anthrone colorimetry, Coomassie brilliant blue colorimetry and fluorescence colorimetry were used to determine the soluble sugar, soluble protein content and vitamin C content of the pepper, respectively. Colorimetry was used to determine the color value of the pepper. The results are shown in Table 3 and Figure 4 、 Figure 5 and Figure 6 .

[0055] Table 3 Effect of microalgae biofertilizer on pepper yield and fruit quality

[0056] The results show that, compared with the control group, the pepper fruits treated with the microalgae biofertilizer of the application grow wider and longer Figure 4 ). The application of the microalgae biofertilizer of the application significantly increases the yield of the pepper by 10.0% Figure 5 In a), the fruit length and width are increased by 11.0% and 13.6% Figure 5 In b, c), the soluble protein, soluble sugar, vitamin C content and color value of the fruit are significantly increased by 20.3%, 10.8%, 62.5% and 19.6% Figure 6 In a-d).

[0057] 4. Determination of physicochemical property indexes of saline-alkali soil On the 45th day after transplanting, the rhizosphere soil of the pepper was collected by the root shaking method. The whole pepper was dug out, and the large soil was shaken off, and the soil closely attached to the root system surface was collected. The soil sample was air-dried, ground, and sieved for physicochemical property determination. The soil and distilled water were mixed at a mass / volume ratio of 1:2.5, and then the soil pH was determined by a pH meter (PHS-3C, Leici, Shanghai). The soil conductivity was determined by a conductivity meter, and the total salt content was determined by the residue drying-mass method. The alkali-hydrolyzable nitrogen in the soil was determined by the alkali-hydrolyzation diffusion method, and the organic matter was determined by the potassium dichromate (K2Cr2O7) volumetric method-dilution heat method. The results are shown in Table 4 and Figure 7 .

[0058] Table 4 Effect of microalgae biofertilizer on soil physicochemical properties

[0059] The results show that, compared with the control group, the application of the microalgae biofertilizer of the application makes the pH, conductivity and total salt content of the soil decrease by 4.8%, 22.2% and 15.4% respectively, and makes the organic matter and alkali-hydrolyzable nitrogen content increase by 29.8% and 13.2% respectively P <0.01; Figure 7 In a-e).

[0060] The above embodiments are the preferred embodiments of the present application, but the embodiments of the present application are not limited to the above embodiments, and any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present application should be equivalent replacement methods, and are all included in the protection scope of the present application.

Claims

1. An Emersonian Lipoglobulus ZDC-1, characterized in that, It was deposited on June 24, 2026, at the China General Microbiological Culture Collection Center (CGMCC), Beijing, China, with accession number CGMCC No. 46972, and classified as *Emersonia lipococcus*. Graesiella emersonii .

2. A microbial culture, characterized in that, It includes Emerson Lipoglobulus ZDC-1 as described in claim 1.

3. The microbial culture according to claim 2, characterized in that, The culture is the fermentation broth of Emerson Lipoglucosium ZDC-1 or its concentrate.

4. A microalgae bio-fertilizer, characterized in that, It comprises Emerson Lipoglucocorticoid ZDC-1 as described in claim 1 and a special carrier liquid; the concentration of Emerson Lipoglucocorticoid ZDC-1 in the microalgae biofertilizer is 1×10⁻⁶. 7 ~ 1×10 8 cells·mL -1 .

5. The microalgae bio-fertilizer according to claim 4, characterized in that, Each liter of the dedicated carrier solution contains the following components: NaNO3 20~30 mg, CaCl2·2H2O 2~3 mg, MgSO4·7H2O 7~8 mg, K2HPO4 7~8 mg, KH2PO4 17~18 mg, NaCl 2~3 mg, Na2EDTA 4~6 mg, FeSO4·7H2O 0.4~0.6 mg, H3BO3 1~2 mg, MnCl2·4H2O 0.5~0.6 mg, ZnSO4·7H2O 0.85~0.95 mg, Na2MoO4·2H2O 0.07~0.09 mg, CuSO4·5H2O 0.14~0.18 mg, and Co(NO3)2·6H2O 0.04~0.06 mg.

6. A method for preparing the microalgae biofertilizer as described in claim 4, characterized in that, Includes the following steps: (1) Cultivate Emerson Lipoglucosidase ZDC-1 as described in claim 1, and collect the wet algal sludge by centrifugation; (2) Prepare the special carrier liquid in the amount specified in claim 5; (3) Mix the wet algal sludge collected in step (1) with the special carrier solution from step (2), and adjust the volume to a concentration of Emerson Lipoglobulin ZDC-1 of 1×10⁻⁶. 7 ~ 1×10 8 cells·mL -1 .

7. The method according to claim 6, characterized in that, The culture described in step (1) uses BG11 liquid medium and is cultured under aeration at a temperature of 25~30℃, light intensity of 2700±50 lx, and light-dark cycle of 14~18h:10~6h until the late logarithmic growth stage.

8. The application of Emerson Lipoglucosidase ZDC-1 as described in claim 1, the microbial culture as described in claim 2, or the microalgae bio-fertilizer as described in claim 4 in promoting chili pepper growth, increasing chili pepper yield, and / or improving saline-alkali soil in saline-alkali land.

9. A method of using the microalgae bio-fertilizer as described in claim 4, characterized in that, Apply by combining root dipping and root irrigation: Before transplanting chili seedlings, completely immerse the roots of the seedlings in the microalgae bio-fertilizer and soak for 20-40 minutes before transplanting to saline-alkali land or potted plants.

10. The method of use according to claim 9, characterized in that, Apply root irrigation once on the 7th, 14th and 21st day after transplanting; each time apply 15-20 mL per plant.

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