Application of gene modules of gibberellin and florigen pathways in improving production efficiency of plant factory

Abstract

The application discloses application of a gene module of a gibberellin pathway and a florigen pathway to improving production efficiency of a plant factory, and belongs to the technical field of biotechnology.The technical problem solved by the application is how to prepare early-maturing high-yield high-quality tomato with dwarf, compact, dense planting, energy-saving and labor-saving in a plant factory.The protein encoded by the disclosed gene of the florigen pathway is shown as SEQ ID No.9 and SEQ ID No.12, and the protein encoded by the gene of the gibberellin pathway is shown as SEQ ID No.3 and SEQ ID No.6.It is found by the application that simultaneous knockout of the genes of the gibberellin pathway and the florigen pathway in tomato can aggregate the gibberellin pathway and the florigen pathway for regulating plant type, and a plant factory suitable tomato with improved production efficiency can be obtained, which is specifically manifested as shortened plant stem, compact crown layer, reduced space occupation volume, saved management time, early maturity and high yield.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology, specifically relating to the application of gene modules of the polymeric gibberellin pathway and the florigen pathway in improving the production efficiency of plant factories. Background Technology

[0002] Frequent extreme weather events, scarce arable land resources, water shortages, and rapid urbanization pose serious threats to traditional agricultural production and food security. Building a more diversified food supply system is an objective requirement and crucial measure for enhancing comprehensive food production capacity and ensuring a stable and secure food supply. Vertical farming, commonly known as plant factories, is a highly efficient agricultural system that integrates environmental control and three-dimensional planting technologies to produce crops in an industrialized manner. Plant factories not only provide diversified solutions for ensuring food security but also possess the dual functions of routine supply and emergency support. Their modular structure allows for rapid deployment after disasters such as earthquakes and floods, maintaining food production in war zones or disaster areas without the need for external supply chains. Mobile containerized plant factories are also adaptable to special environments such as submarines, warships, islands, and high-altitude cold regions, effectively strengthening border defense logistics capabilities. Benefiting from their independent and enclosed production characteristics, plant factories also have the potential to provide sustainable food supply solutions for future space engineering projects.

[0003] However, the widespread application of plant factories is still limited by factors such as high facility investment and operating costs, high energy consumption, and a lack of specialized varieties. In particular, the lack of suitable varieties leads to the situation where "oranges grow north of the Huai River," further increasing the operating costs of plant factories. Therefore, the lagging progress in the innovation of suitable germplasm resources has become a core bottleneck restricting the improvement of plant factory production efficiency, the root cause of which lies in the scarcity of available breeding genetic resources. Therefore, exploring breeding genetic resources suitable for plant factories is the fundamental solution for breeding suitable varieties and improving the production efficiency of plant factories.

[0004] tomato( Solanum lycopersicum Tomatoes are the world's largest fruit and vegetable crop and an important model crop among solanaceous crops. Traditional agricultural tomato varieties have poor plant structure and growth cycles that are not well-suited to the plant factory environment, resulting in high energy consumption, increased labor costs, and low yields, severely restricting production efficiency. Therefore, there is an urgent need to integrate new genetic resources and breeding technologies to improve the production efficiency of tomatoes in plant factories.

[0005] SP and SP5G The gene encodes antiflorigen in tomatoes, which inhibits tomato flowering by antagonizing the florigen signal.

[0006] Gibberellin (GA) is an important hormone regulating plant growth and development. It is synthesized through a stepwise catalytic reaction involving ent-copalyl diphosphate synthase (CPS), ent-kaurene synthase (KS), ent-kaurenoic acid oxidase (KAO), ent-kaurene oxidase (KO), GA-20 oxidase (GA20ox), and GA-3 oxidase (GA3ox). GA-2 oxidase (GA2ox) can convert active GA into inactive GA. Inhibition of each step in the GA biosynthetic pathway leads to changes in GA levels within the plant. After GA binds to its receptor GID1 (GIBBERELLIN-INSENSITIVE DWARF1), it can form a complex with the growth inhibitory protein DELLA, thereby promoting the degradation of DELLA by the E3 ubiquitin ligase SLY1 (GID2 / SLEEPY1), relieving the growth inhibitory effect of DELLA, and promoting plant growth and development. Summary of the Invention

[0007] The technical problem to be solved by this invention is how to prepare early-maturing, high-yielding, and high-quality tomatoes that are short-stemmed, compact, densely planted, energy-saving, and labor-saving, thereby improving the production efficiency of plant factories.

[0008] To address the aforementioned technical problems, the present invention first provides at least one of the following applications of fibrinolytic pathway-related proteins or substances regulating the content or activity of said fibrinolytic pathway-related proteins: X1) Regulates plant architecture; X2) Prepare products that regulate plant architecture; X3) Regulates the ripening time of plant fruits; X4) Prepare products that regulate the ripening time of plant fruits; X5) Regulates plant fruit yield; X6) Prepare products that regulate plant fruit yield; X7) Cultivating plants with altered plant shape; X8) Prepare products from plants with altered plant type; X9) Cultivating plants whose fruit ripening time is altered; X10) Prepare products from plants that alter fruit ripening time; X11) Cultivating plants that alter fruit yield; X12) Prepare products from plants that alter fruit yield; The proteins related to the florigen molecular pathway are derived from tomatoes.Solanum lycopersicum ), which are SP proteins and / or SP5G proteins, wherein the SP proteins are as follows: A1), A2), or A3). A1) The amino acid sequence of this protein is SEQ ID No. 9; A2) A protein with the same function as the amino acid sequence shown in SEQ ID No. 9 in the sequence listing, but with amino acid residue substitution and / or deletion and / or addition; A3) A fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of A1) or A2); The SP5G protein is either B1), B2), or B3). B1) The amino acid sequence of this protein is that of SEQ ID No. 12; B2) A protein with the same function as the amino acid sequence shown in SEQ ID No. 12 in the sequence listing, but with amino acid residue substitution and / or deletion and / or addition; B3) A fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of A1) or A2).

[0009] The SP protein in A2) above is a protein that shares 75% or more amino acid sequence identity with the protein shown in SEQ ID No. 9 and has the same function. The SP5G protein in B2) above is a protein that shares 75% or more amino acid sequence identity with the protein shown in SEQ ID No. 12 and has the same function. Identity refers to the similarity of amino acid sequences. The similarity of amino acid sequences can be determined using homology search sites on the Internet, such as the BLAST page on the NCBI homepage. For example, in Advanced BLAST 2.1, using blastp as the program, setting the Expect value to 10, setting all filters to OFF, using BLOSUM62 as the matrix, setting the Gap existence cost, Per residue gap cost, and Lambda ratio to 11, 1, and 0.85 (default values) respectively, and performing a search for the similarity of a pair of amino acid sequences to calculate the similarity value (%). The phrase "having 75% or more of the sameness" means having 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% of the sameness.

[0010] In the above applications, the substance that regulates the content or activity of the proteins related to the florigen molecular pathway can be a substance that regulates the content or activity of the SP protein and / or a substance that regulates the content or activity of the SP5G protein. The substance that regulates the content or activity of the SP protein is any one of the following C1) to C9): C1) The nucleic acid molecule encoding the SP protein; C2) An expression cassette containing the nucleic acid molecule described in C1); C3) A recombinant vector containing the nucleic acid molecule described in C1), or a recombinant vector containing the expression cassette described in C2); C4) Recombinant microorganisms containing the nucleic acid molecules described in C1), or recombinant microorganisms containing the expression cassette described in C2), or recombinant microorganisms containing the recombinant vector described in C3); C5) A transgenic plant cell line containing the nucleic acid molecule described in C1), or a transgenic plant cell line containing the expression cassette described in C2); C6) Transgenic plant tissue containing the nucleic acid molecules described in C1), or transgenic plant tissue containing the expression cassette described in C2); C7) A transgenic plant organ containing the nucleic acid molecule described in C1), or a transgenic plant organ containing the expression cassette described in C2); C8) Nucleic acid molecules that reduce the content or activity of the SP protein; C9) Expression cassettes, recombinant vectors, recombinant microorganisms, transgenic plant cell lines, transgenic plant tissues, or transgenic plant organs containing the nucleic acid molecules described in C8); The substance that regulates the content or activity of the SP5G protein is any one of the following D1) to D9): D1) The nucleic acid molecule encoding the SP5G protein; D2) An expression cassette containing the nucleic acid molecules described in D1); D3) A recombinant vector containing the nucleic acid molecule described in D1), or a recombinant vector containing the expression cassette described in D2); D4) Recombinant microorganisms containing the nucleic acid molecules described in D1), or recombinant microorganisms containing the expression cassette described in D2), or recombinant microorganisms containing the recombinant vector described in D3); D5) A transgenic plant cell line containing the nucleic acid molecule described in D1), or a transgenic plant cell line containing the expression cassette described in D2); D6) Transgenic plant tissue containing the nucleic acid molecules described in D1), or transgenic plant tissue containing the expression cassette described in D2); D7) Transgenic plant organs containing the nucleic acid molecules described in D1), or transgenic plant organs containing the expression cassette described in D2); D8) Nucleic acid molecules that reduce the content or activity of the SP5G protein; D9) Expression cassettes, recombinant vectors, recombinant microorganisms, transgenic plant cell lines, transgenic plant tissues, or transgenic plant organs containing the nucleic acid molecules described in D8).

[0011] The nucleic acid molecule can be DNA, such as cDNA, genomic DNA, or recombinant DNA; the nucleic acid molecule can also be RNA, such as mRNA or hnRNA.

[0012] In the above applications, the nucleic acid molecule described in C1) can be a DNA molecule whose coding sequence is SEQ ID No. 8; D1) The nucleic acid molecule may be a DNA molecule whose coding sequence is SEQ ID No. 11.

[0013] Specifically, the nucleic acid molecule described in C1) may be the DNA molecule shown in SEQ ID No. 8 or SEQ ID No. 7; D1) The nucleic acid molecule may be the DNA molecule shown in SEQ ID No. 11 or SEQ ID No. 10.

[0014] In the above applications, the substance that regulates the content or activity of proteins related to the florigen molecular pathway can be a substance that reduces or increases the content or activity of proteins related to the florigen molecular pathway.

[0015] Specifically, the substance that regulates the content or activity of proteins related to the florigen molecular pathway can be a substance that reduces the content or activity of proteins related to the florigen molecular pathway; the substance that regulates plant form can be a substance that reduces plant form; the substance that regulates fruit ripening time can be a substance that shortens fruit ripening time; the substance that regulates fruit yield can be a substance that increases fruit yield; the substance that cultivates plants with altered plant form can be a plant with a reduced plant form; the substance that cultivates plants with altered fruit ripening time can be a plant with a shortened fruit ripening time; and the substance that cultivates plants with altered fruit yield can be a plant with increased fruit yield.

[0016] In the above applications, the vector can be a plasmid, granule, bacteriophage, or viral vector.

[0017] C8) The nucleic acid molecule that reduces SP content may be an sgRNA that targets the coding gene of SP.

[0018] The recombinant vector described in C9) may be a recombinant vector prepared using the CRISPER / Cas9 system capable of editing the SP gene. The recombinant vector may express sgRNA targeting the nucleic acid molecule described in C1). The target sequence of the sgRNA may be positions 1094-1113 of SEQ ID No. 7.

[0019] D8) The nucleic acid molecule that reduces SP5G content may be an sgRNA that targets the coding gene of SP5G.

[0020] D9) The recombinant vector may be a recombinant vector prepared using the Crisper / Cas9 system capable of editing the SP5G gene. The recombinant vector may express sgRNA targeting the nucleic acid molecule described in D1). The target sequence of the sgRNA may be positions 1119-1138 of SEQ ID No. 10.

[0021] In the above applications, the microorganisms can be yeast, bacteria, algae, or fungi. Among them, bacteria can be Agrobacterium, such as Agrobacterium AGL1.

[0022] In the above applications, the transgenic plant cell lines, transgenic plant tissues, and transgenic plant organs do not include propagation material.

[0023] The present invention also provides an application of at least one of the following X1)-X12) of the composition: X1) Regulates plant architecture; X2) Prepare products that regulate plant architecture; X3) Regulates the ripening time of plant fruits; X4) Prepare products that regulate the ripening time of plant fruits; X5) Regulates plant fruit yield; X6) Prepare products that regulate plant fruit yield; X7) Cultivating plants with altered plant shape; X8) Prepare products from plants with altered plant type; X9) Cultivating plants whose fruit ripening time is altered; X10) Prepare products from plants that alter fruit ripening time; X11) Cultivating plants that alter fruit yield; X12) Prepare products from plants that alter fruit yield; The composition consists of P1) and P2): P1) The floridin molecular pathway-related protein or the substance that regulates the content or activity of the floridin molecular pathway-related protein; P2) Gibberellin molecular pathway-related proteins or substances that regulate the content or activity of said gibberellin molecular pathway-related proteins.

[0024] Furthermore, the gibberellin molecular pathway-related protein may be a gibberellin biosynthesis pathway-related protein and / or a gibberellin signal transduction pathway-related protein.

[0025] Specifically, the proteins related to the gibberellin biosynthesis pathway may be one or more of the following expressed in tomato vegetative meristems, stems, or leaves: GA-20 oxidase (GA20ox), GA-2 oxidase (GA2ox), GA-3 oxidase (GA3ox), ent-copalyl diphosphate synthase (CPS), ent-kaurene synthase (KS), ent-kaurenoic acid oxidase (KAO), and / or ent-kaurene oxidase (KO). See Table 7. SlGA3ox1、 SlGA3ox2、SlGA3ox3、SlGA3ox4、SlGA20ox3、SlGA20ox4、SlGA20ox7、SlGA2ox2、SlGA2ox6、 SlGA2ox7、 SlGA2ox8、GIB-1、SlCPS-like、GIB-3、GIB-2、SlKAO-like3、SlKO One or more of them.

[0026] The gibberellin signal transduction pathway-related proteins may be gibberellin receptors GID1a, GID1b1, and / or GID1b2, expressed in tomato vegetative meristems, stems, or leaves; the E3 ubiquitin ligase SLY1; and / or the tomato delta protein PROCERA. (See Table 7 for examples.) GID1a、GID1b1、GID1b2、PRO / SlDELLA、SLY1 One or more of them.

[0027] Furthermore, the GA-20 oxidase may be SlGA20ox1 protein and / or SlGA20ox2 protein, and the substance that regulates the content or activity of the gibberellin molecular pathway-related proteins may be a substance that regulates the content or activity of the SlGA20ox1 protein and / or a substance that regulates the content or activity of the SlGA20ox2 protein. The SlGA20ox1 protein is as follows: (E1), (E2), or (E3) E1) The amino acid sequence of this protein is that of SEQ ID No. 3; E2) A protein with the same function as the amino acid sequence shown in SEQ ID No. 3 in the sequence listing, but with the substitution and / or deletion and / or addition of amino acid residues; E3) is a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of E1) or E2); The SlGA20ox2 protein is either G1), G2), or G3). G1) The amino acid sequence of this protein is that of SEQ ID No. 6; G2) A protein with the same function obtained by substituting and / or deleting and / or adding amino acid residues of the amino acid sequence shown in SEQ ID No. 6 in the sequence listing; G3) is a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of G1) or G2); The substance that regulates the content or activity of the SlGA20ox1 protein is any one of the following (H1) to (H9): H1) The nucleic acid molecule encoding the SlGA20ox1 protein; H2) contains an expression cassette containing the nucleic acid molecule described in H1); H3) a recombinant vector containing the nucleic acid molecule described in H1), or a recombinant vector containing the expression cassette described in H2); H4) Recombinant microorganisms containing the nucleic acid molecules described in H1), or recombinant microorganisms containing the expression cassette described in H2), or recombinant microorganisms containing the recombinant vector described in H3); H5) A transgenic plant cell line containing the nucleic acid molecule described in H1), or a transgenic plant cell line containing the expression cassette described in H2); H6) Transgenic plant tissue containing the nucleic acid molecules described in H1), or transgenic plant tissue containing the expression cassette described in H2); H7) Transgenic plant organs containing the nucleic acid molecules described in H1), or transgenic plant organs containing the expression cassette described in H2; H8) Nucleic acid molecules that reduce the content or activity of the SlGA20ox1 protein; H9) Expression cassettes, recombinant vectors, recombinant microorganisms, transgenic plant cell lines, transgenic plant tissues or transgenic plant organs containing the nucleic acid molecules described in H8); The substance that regulates the content or activity of the SlGA20ox2 protein is any one of the following (I1) to (I9): I1) The nucleic acid molecule encoding the SlGA20ox2 protein; I2) An expression cassette containing the nucleic acid molecule described in I1); I3) A recombinant vector containing the nucleic acid molecule described in I1), or a recombinant vector containing the expression cassette described in I2); I4) Recombinant microorganisms containing the nucleic acid molecules described in I1), or recombinant microorganisms containing the expression cassette described in I2), or recombinant microorganisms containing the recombinant vector described in I3); I5) A transgenic plant cell line containing the nucleic acid molecule described in I1), or a transgenic plant cell line containing the expression cassette described in I2); I6) Transgenic plant tissue containing the nucleic acid molecules described in I1), or transgenic plant tissue containing the expression cassette described in I2); I7) Transgenic plant organs containing the nucleic acid molecules described in I1), or transgenic plant organs containing the expression cassette described in I2); I8) Nucleic acid molecules that reduce the content or activity of the SlGA20ox2 protein; I9) Expression cassettes, recombinant vectors, recombinant microorganisms, transgenic plant cell lines, transgenic plant tissues or transgenic plant organs containing the nucleic acid molecules described in I8).

[0028] The SlGA20ox1 protein in E2) above is a protein that shares 75% or more of the amino acid sequence identity with the protein shown in SEQ ID No. 3 and has the same function. The SlGA20ox2 protein in G2) above is a protein that shares 75% or more of the amino acid sequence identity with the protein shown in SEQ ID No. 6 and has the same function. The 75% or more of identity is defined as 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity.

[0029] The nucleic acid molecule can be DNA, such as cDNA, genomic DNA, or recombinant DNA; the nucleic acid molecule can also be RNA, such as mRNA or hnRNA.

[0030] In the above applications, the nucleic acid molecule described in H1) can be a DNA molecule whose coding sequence is SEQ ID No. 2; I1) The nucleic acid molecule may be a DNA molecule whose coding sequence is SEQ ID No. 5.

[0031] Specifically, the nucleic acid molecule described in H1) may be the DNA molecule shown in SEQ ID No. 2 or SEQ ID No. 1; I1) The nucleic acid molecule may be the DNA molecule shown in SEQ ID No. 5 or SEQ ID No. 4.

[0032] In the application of the above composition, the substance regulating the content or activity of proteins related to the florigen molecular pathway can be a substance that decreases or increases the content or activity of proteins related to the florigen molecular pathway. Similarly, the substance regulating the content or activity of proteins related to the gibberellin molecular pathway can be a substance that decreases or increases the content or activity of proteins related to the gibberellin molecular pathway.

[0033] Specifically, the substance regulating the content or activity of proteins related to the florigen molecular pathway can be a substance that reduces the content or activity of proteins related to the florigen molecular pathway; the substance regulating the content or activity of proteins related to the gibberellin molecular pathway can be a substance that reduces the content or activity of proteins related to the gibberellin molecular pathway; the regulation of plant form can be a reduction in plant form; the regulation of plant fruit ripening time can be a shortening of plant fruit ripening time; the regulation of plant fruit yield can be an increase in plant fruit yield; the cultivation of plants with altered plant form can be a cultivation of plants with reduced plant form; the cultivation of plants with altered fruit ripening time can be a cultivation of plants with shortened fruit ripening time; and the cultivation of plants with altered fruit yield can be a cultivation of plants with increased fruit yield.

[0034] The present invention also provides at least one application of the SlGA20ox1 protein, or the substance that regulates the content or activity of the SlGA20ox1 protein, of the following Y1)-Y12): Y1) Regulates plant architecture; Y2) Prepare products that regulate plant architecture; Y3) Regulates the ripening time of plant fruits; Y4) Prepare products that regulate the ripening time of plant fruits; Y5) Cultivating plants with altered plant shape; Y6) Prepare products from plants with altered plant type; Y7) Cultivating plants whose fruit ripening time can be altered; Y8) Prepare products from plants that alter the fruit ripening time; Y9) regulates plant fruit yield and at least one of Y1)-Y8); Y10) prepares a product that regulates plant fruit yield and at least one of Y1)-Y8); Y11) Cultivation of plants that alter fruit yield and at least one of Y1)-Y8); Y12) is a product of preparing plants that alter fruit yield and at least one of Y1)-Y8).

[0035] The substance that regulates the content or activity of the SlGA20ox1 protein can be a substance that reduces or increases the content or activity of the SlGA20ox1 protein.

[0036] Specifically, the substance that regulates the content or activity of the SlGA20ox2 protein can be a substance that reduces the content or activity of the SlGA20ox2 protein; the substance that regulates plant form can be a substance that reduces plant form; the substance that regulates fruit ripening time can be a substance that shortens fruit ripening time; the substance that regulates fruit yield can be a substance that increases fruit yield; the substance that cultivates plants with altered plant form can be a plant with a reduced plant form; the substance that cultivates plants with altered fruit ripening time can be a plant with a shortened fruit ripening time; and the substance that cultivates plants with altered fruit yield can be a plant with increased fruit yield.

[0037] The present invention also provides at least one application of the SlGA20ox2 protein, or the substance that regulates the content or activity of the SlGA20ox2 protein, of the following X1)-X12): X1) Regulates plant architecture; X2) Prepare products that regulate plant architecture; X3) Regulates the ripening time of plant fruits; X4) Prepare products that regulate the ripening time of plant fruits; X5) Regulates plant fruit yield; X6) Prepare products that regulate plant fruit yield; X7) Cultivating plants with altered plant shape; X8) Prepare products from plants with altered plant type; X9) Cultivating plants whose fruit ripening time is altered; X10) Prepare products from plants that alter fruit ripening time; X11) Cultivating plants that alter fruit yield; X12) is used to prepare products from plants that produce altered fruit yields.

[0038] The substance that regulates the content or activity of the SlGA20ox2 protein can be a substance that reduces or increases the content or activity of the SlGA20ox2 protein.

[0039] Specifically, the substance that regulates the content or activity of the SlGA20ox2 protein can be a substance that reduces the content or activity of the SlGA20ox2 protein; the substance that regulates plant form can be a substance that reduces plant form; the substance that regulates fruit ripening time can be a substance that shortens fruit ripening time; the substance that regulates fruit yield can be a substance that increases fruit yield; the substance that cultivates plants with altered plant form can be a plant with a reduced plant form; the substance that cultivates plants with altered fruit ripening time can be a plant with a shortened fruit ripening time; and the substance that cultivates plants with altered fruit yield can be a plant with increased fruit yield.

[0040] The present invention also provides any of the following methods: Z1) Methods for reducing plant size, shortening fruit ripening time and / or increasing fruit yield, including: reducing the content or activity of the proteins related to the florigen molecular pathway in the plant, or knocking out the coding gene of the protein related to the florigen molecular pathway in the plant, or reducing the expression level of the coding gene of the protein related to the florigen molecular pathway in the plant, thereby reducing plant size, shortening fruit ripening time and / or increasing fruit yield. Z2) Methods for cultivating plants with reduced plant size, shortened fruit ripening time and / or increased fruit yield, including: reducing the content or activity of the proteins related to the flowering pathway in the plant, or knocking out the coding gene of the protein related to the flowering pathway in the plant, or reducing the expression level of the coding gene of the protein related to the flowering pathway in the plant, to obtain the target plant with reduced plant size, shortened fruit ripening time and / or increased fruit yield. Z3) Methods for reducing plant size, shortening fruit ripening time, and / or increasing fruit yield, including: reducing the content or activity of the floridin molecular pathway-related proteins and the gibberellin molecular pathway-related proteins in the plant, or knocking out the coding genes of the floridin molecular pathway-related proteins and the gibberellin molecular pathway-related proteins in the plant, or reducing the expression level of the coding genes of the floridin molecular pathway-related proteins and the gibberellin molecular pathway-related proteins in the plant, thereby reducing plant size, shortening fruit ripening time, and / or increasing fruit yield; Z4) Methods for cultivating plants with reduced plant size, shortened fruit ripening time, and / or increased fruit yield, including: reducing the content or activity of the floridin molecular pathway-related proteins and the gibberellin molecular pathway-related proteins in the plant, or knocking out the coding genes of the floridin molecular pathway-related proteins and the gibberellin molecular pathway-related proteins in the plant, or reducing the expression level of the coding genes of the floridin molecular pathway-related proteins and the gibberellin molecular pathway-related proteins in the plant, to obtain the target plant with reduced plant size, shortened fruit ripening time, and / or increased fruit yield; Z5) Methods for reducing plant size, shortening fruit ripening time and / or increasing fruit yield, including: reducing the content or activity of the SlGA20ox1 protein in the plant, or knocking out the coding gene of the SlGA20ox1 protein in the plant, or reducing the expression level of the coding gene of the SlGA20ox1 protein in the plant, thereby reducing plant size, shortening fruit ripening time and / or increasing fruit yield. Z6) Methods for cultivating plants with reduced plant size, shortened fruit ripening time and / or increased fruit yield, including: reducing the content or activity of the SlGA20ox1 protein in the plant, or knocking out the gene encoding the SlGA20ox1 protein in the plant, or reducing the expression level of the gene encoding the SlGA20ox1 protein in the plant, to obtain the target plant with reduced plant size, shortened fruit ripening time and / or increased fruit yield. Z7) Methods for reducing plant size, shortening fruit ripening time and / or increasing fruit yield, including: reducing the content or activity of the SlGA20ox2 protein in the plant, or knocking out the coding gene of the SlGA20ox2 protein in the plant, or reducing the expression level of the coding gene of the SlGA20ox2 protein in the plant, thereby reducing plant size, shortening fruit ripening time and / or increasing fruit yield. Z8) Methods for cultivating plants with reduced plant size, shortened fruit ripening time and / or increased fruit yield, including: reducing the content or activity of the SlGA20ox2 protein in the plant, or knocking out the coding gene of the SlGA20ox2 protein in the plant, or reducing the expression level of the coding gene of the SlGA20ox2 protein in the plant, to obtain the target plant with reduced plant size, shortened fruit ripening time and / or increased fruit yield.

[0041] In the above methods, Z1)-Z8) can be achieved by editing or mutagenesis of the gene encoding the target protein. The editing can be performed using methods such as CRISPR / Cas9, Prime editing, or Base editing, which can mutate the gene (e.g., missense mutations, frameshift mutations, nonsense mutations, mutations leading to premature stop codons, splicing errors, insertions, deletions, or duplications). The mutagenesis can be performed using methods such as ethyl methanesulfonate (EMS) or radiation mutagenesis, which can mutate the gene (e.g., missense mutations, frameshift mutations, nonsense mutations, mutations leading to premature stop codons, splicing errors, insertions, deletions, or duplications).

[0042] Gene editing of the coding gene using the CRISPR / Cas9 method can be achieved by introducing a recombinant vector (such as pAGM4723-ox1, pAGM4723-ox2, pAGM4723-SP-SP5G) containing the sgRNA of the gene encoding Cas9 that can be transcribed to target proteins into plants and then screening to obtain the target plant whose coding gene has been edited.

[0043] The target plant is understood to include not only the first-generation plant in which the target protein or its encoding gene has been altered, but also its progeny. For the target plant, the gene can be propagated within the species, or it can be transferred into other varieties of the same species, particularly commercial varieties, using conventional breeding techniques. The target plant includes seeds, callus tissue, intact plants, and cells.

[0044] In one embodiment of the present invention, the target plant is slga20ox1 The mutant is missing ATTCA at positions 208-212 of SEQ ID No. 1.

[0045] In one embodiment of the present invention, the target plant is slga20ox2 The mutant has an A inserted between positions 434 and 435 of SEQ ID No. 4 and a T inserted between positions 986 and 987 of SEQ ID No. 4.

[0046] In one embodiment of the present invention, the target plant is sp sp5g mutants, their SP The TGT gene is deleted from positions 1108-1110 of SEQ ID No. 7. SP5G Gene Deletion SEQ ID GTTTACAACAATAGGGTGGTCTATAATGGATGTTCCTTGAGGCCTTCACAAGTTGTCAATCAACCTAGGGTTGACATTGATGGAGACGATCTTCGTACTTTTTACACTC at positions 1082-1316 of No.10 TGGTATAAACTCATCGTTTTATTTCATATGATATACATATATATATATATATATATATATATATATATTTTCTTTCTATTTATACATTTTAATATCTCTAAATTATTAACCTTTTGTCAA.

[0047] In one embodiment of the present invention, the target plant is sp sp5g slga20ox1 mutant, which is slga20ox1 mutants and sp sp5g Hybrid offspring of mutants.

[0048] In one embodiment of the present invention, the target plant is sp sp5g slga20ox2 mutant, which is slga20ox2 mutants and sp sp5g Hybrid offspring of mutants.

[0049] The following products are also within the scope of protection of this invention: R1) The floridin molecular pathway-related protein, or the substance that regulates the content or activity of the floridin molecular pathway-related protein; R2) The composition according to any one of claims 5-9; R3) the SlGA20ox1 protein, or the substance that regulates the content or activity of the SlGA20ox1 protein; R4) the SlGA20ox2 protein, or the substance that regulates the content or activity of the SlGA20ox2 protein.

[0050] In this invention, the plant type can be reflected in stem length, ear height, leaf size, canopy height and / or plant volume.

[0051] In this invention, the fruit yield can be the fruit yield per plant.

[0052] In this invention, the fruit yield can specifically refer to the fruit yield in a plant factory.

[0053] In this invention, the plant can be a plant growing in a limited space (such as a plant factory or greenhouse facility that is not outdoors) or a plant growing in an unlimited space (such as an outdoor field environment).

[0054] Furthermore, the limited space can be, but is not limited to, facility agriculture environments such as plant factories or greenhouses.

[0055] In this invention, the plant can be any one of M1)-M6): M1) Angiosperms; M2) Dicotyledons; M3) Solanales plants; M4) Solanaceae plants; M5) Solanaceae plants; M6 tomatoes.

[0056] This invention discovers that simultaneously knocking out the coding genes for proteins related to the florigen molecular pathway and the gibberellin molecular pathway can aggregate the gibberellin and florigen pathways that regulate plant architecture, resulting in a plant factory-suitable tomato with improved production efficiency. Specifically, this manifests as shorter plant stems, more compact canopies, reduced space occupation, reduced management time, earlier maturity, and higher yield. This provides a revolutionary gene module for vertical agriculture, enabling the rapid creation of customized plant factory-specific varieties of solanaceous vegetables.

[0057] The present invention will now be described in further detail with reference to specific embodiments. The given embodiments are merely illustrative of the invention and not intended to limit its scope. The embodiments provided below can serve as a guide for further improvements by those skilled in the art and do not constitute a limitation on the invention in any way. Attached Figure Description

[0058] Figure 1 Targeting CRISPR / Cas9 SlGA20ox1 Gene (C) SlGA20ox2 Gene (D) SP Gene (A) and SP5G A schematic diagram of the target of gene (B) and the results of mutant genotype identification.

[0059] Figure 2 for sp sp5g , sp sp5g slga20ox1, sp sp5g slga20ox2 Phenotypic effects of mutants on production efficiency in plant factories. A represents plant appearance (bar value 10 cm); B represents the appearance of plant stems and fruits after leaf removal (bar value 10 cm); C represents the measurement of primary stem length; D represents the measurement of sympodial stem length; E represents the measurement of fruit cluster height; F represents the measurement of the length of the first leaf below the first inflorescence; G represents the measurement of the diameter at the widest point of the plant in the horizontal direction; H represents the measurement of the space occupied by a single plant; I represents the statistical result of the number of days for the first fruit to mature; J represents the statistical result of the number of fruits per plant; K represents the statistical result of the statistical result of the yield of mature fruits per plant; L represents the statistical result of management time. The horizontal axis in CL represents WT from left to right. sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 ".

[0060] Figure 3 This represents the gene expression pattern of the gibberellin pathway. The numbers in the squares represent the gene expression level in the tissue, expressed in Reads Per Kilobase per Million mapped reads (RPKM). Detailed Implementation

[0061] In this document, unless otherwise defined herein, terms should be understood according to their common usage by those skilled in the art. Examples of resources describing many of the molecular biology-related terms used herein can be found in the following references: Alberts et al., Molecular Biology of The Cell, 5th ed., Garland Science Publishing, Inc.: New York, 2007; Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed., Springer-Verlag: New York, 1991; King et al., A Dictionary of Genetics, 6th ed., Oxford University Press: New York, 2002; and Lewin, GenesIX, Oxford University Press: New York, 2007.

[0062] Unless otherwise stated, the terms “nucleic acid,” “nucleotide,” and “polynucleotide” cover both DNA and RNA.

[0063] The term "vector" refers to a nucleic acid molecule that allows the insertion of foreign nucleic acids without disrupting its ability to replicate and / or integrate into the host cell. A vector may include a nucleic acid sequence that allows it to replicate in the host cell, such as an origin of replication. A vector may also include one or more optional marker genes and other genetic elements. An integration vector is capable of integrating itself into the host nucleic acid. An expression vector is a vector that contains the necessary regulatory sequences to allow the transcription and translation of the inserted gene. Vectors can be plasmid vectors or viral vectors. The term "plasmid" refers to a structure composed of genetic material designed to guide the transformation of target cells. A plasmid includes a plasmid backbone. The plasmid backbone contains multiple genetic elements that are localized and sequentially oriented with other essential genetic elements, enabling the nucleic acids in the nucleic acid cassette to be transcribed in the transfected cell and translated where necessary. The plasmid backbone may contain one or more unique nucleic acid restriction sites. A plasmid is capable of autonomous replication in a host or organism, thereby replicating a cloned sequence. A plasmid can confer certain phenotypes on the host organism that are selective or easily detectable. A plasmid or plasmid backbone may have a linear or circular conformation. The constituent elements of a plasmid may include, but are not limited to, the following DNA molecules: (1) DNA; (2) the plasmid backbone; (3) the sequence encoding the target gene; and (4) regulatory elements responsible for transcription, translation, RNA stability, and replication.

[0064] The term "import" refers to the operation of transferring the gene or a recombinant vector containing the gene into a cell so that the gene can be expressed in the cell.

[0065] The expression can be transient, continuous, or stable.

[0066] The term "transient expression" refers to the introduction of genetic material that does not integrate into the host cell genome or is not replicated, and therefore can be degraded or transferred to other compartments over a period of time.

[0067] The term "sustained expression" refers to the introduction of a target gene along with genetic elements into a cell that enable the genetic material to replicate and / or be maintained outside the cell (i.e., extrachromosomally). This can lead to a remarkably stable transformation of the cell without integrating the new genetic material into the host cell's chromosome.

[0068] The term "stable expression" refers to the introduction of genetic material into the chromosome of a target cell, where it integrates and becomes a permanent component of the cell's genetic material. Stable gene expression after introduction can permanently alter the characteristics of the cell and its offspring produced through replication, leading to stable transformation.

[0069] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials, reagents, instruments, etc., used in the following examples are commercially available.

[0070] BsaI restriction enzyme, 10× Cutsmart buffer, T4 ligase, and 10× T4 ligase buffer are all products of NEB. BpiI restriction enzyme is a Thermo product.

[0071] Ailsa Craig (AC) is a classic tomato cultivar, listed in the USDA Germplasm Bank with the accession number PI 262995.

[0072] Example 1, for SlGA20ox1 , SlGA20ox2 , SP and SP5G Gene editing can produce compact, early-maturing, and high-yielding tomatoes. This embodiment found that by analyzing tomatoes ( Solanum lycopersicum ) of SlGA20ox1 Gene, SlGA20ox2 Gene, SP Genes and SP5GGene editing can produce dwarf, compact, densely planted, energy-efficient, and labor-saving early-maturing, high-yielding, and high-quality tomatoes, significantly improving tomato production efficiency in plant factories. In the tomato variety Alisa Craig, SlGA20ox1 The genome sequence of the gene is shown in SEQ ID No. 1, and its CDS sequence is shown in SEQ ID No. 2, encoding the SlGA20ox1 protein shown in SEQ ID No. 3; SlGA20ox2 The genome sequence of the gene is shown in SEQ ID No. 4, and its CDS sequence is shown in SEQ ID No. 5, encoding the SlGA20ox2 protein shown in SEQ ID No. 6; SP The genome sequence of the gene is shown in SEQ ID No. 7, and its CDS sequence is shown in SEQ ID No. 8, encoding the SP protein shown in SEQ ID No. 9; SP5G The genome sequence of the gene is shown in SEQ ID No. 10, and its CDS sequence is shown in SEQ ID No. 11, encoding the SP5G protein shown in SEQ ID No. 12.

[0073] I. Preparation of Gene Editing Vectors 1. Design of target sequences exist SlGA20ox1 Two target sites were designed in the first exon region of the gene (Solyc03g006880) (e.g., Figure 1 The target sequence is as follows: SlGA20ox1 -Target1: 5'-GTCAGGCCATATGAATTGTG-3' (PAM is TGG, corresponding to the antisense complementary sequence of positions 204-223 of SEQ ID No. 1); SlGA20ox1 -Target2: 5'-GCTTGTTGGGGAAGCGTGTC-3' (PAM is GGG, corresponding to the sequence of positions 325-344 of SEQ ID No. 1).

[0074] exist SlGA20ox2 Two target sites were designed in the first exon region of the gene (Solyc06g035530) (e.g., Figure 1 The target sequence is as follows: SlGA20ox2 -Target1: 5'-AGGAAGAGAGGAGATCATTG-3' (PAM is TGG, corresponding to the sequence of positions 418-437 of SEQ ID No. 4); SlGA20ox2-Target2: 5'-CGATGAGCACTCTCTCTTCT-3' (PAM is GGG, corresponding to the sequence of positions 969-988 of SEQ ID No. 4).

[0075] exist SP Design one target site (e.g., in the first exon region of gene (Solyc06g074350)) Figure 1 The target sequence is as follows: SP -Target: 5'-ATGTGTGAACCCCTTGTGAT-3' (PAM is TGG, corresponding to the sequence of SEQ ID No. 7, positions 1094-1113).

[0076] exist SP5G Design one target site (e.g., in the first exon region of gene (Solyc05g053850)) Figure 1 The target sequence is as follows: SP5G -Target: 5'-GACAACTTGTGAAGGCCTCA-3' (PAM is AGG, corresponding to the antisense complementary sequence at positions 1119-1138 of SEQ ID No. 10).

[0077] 2. Construction of recombinant plasmids 1) Synthesize the following single-stranded primers containing the adapter sequence (underlined part): SlGA20ox1-guide1-F: ATTG GTCAGGCCATATGAATTGTG; SlGA20ox1-guide1-R: AAAC CACAATTCATATGGCCTGAC; SlGA20ox1-guide2-F: ATTG GCTTGTTGGGGAAGCGTGTC; SlGA20ox1-guide2-R: AAAC GACACGCTTCCCCAACAAGC; SlGA20ox2-guide1-F: ATTG AGGAAGAGAGGAGATCATTG; SlGA20ox2-guide1-R: AAAC CAATGATCTCCTCTCTTCCT; SlGA20ox2-guide2-F: ATTG CGATGAGCACTCTCTCTTCT; SlGA20ox2-guide2-R: AAAC AGAAGAGAGAGAGTGCTCATCG; SP-guide-F: ATTG ATGTGTGAACCCCTTGTGAT; SP-guide-R: AAAC ATCACAAGGGGTTCACACAT; SP5G-guide-F: ATTG GACAACTTGTGAAGGCCTCA; SP5G-guide-R: AAAC TGAGGCCTTCACAAGTTGTC.

[0078] Annealing SlGA20ox1-guide1-F with SlGA20ox1-guide1-R yields the double-stranded DNA molecule SlGA20ox1-guide1; annealing SlGA20ox1-guide2-F with SlGA20ox1-guide2-R yields the double-stranded DNA molecule SlGA20ox1-guide2; annealing SlGA20ox2-guide1-F with SlGA20ox2-guide1-R yields the double-stranded DNA molecule SlGA20ox2-guide1; annealing SlGA20ox2-guide2-F with SlGA20ox2-guide2-R yields the double-stranded DNA molecule SlGA20ox2-guide2; annealing SP-guide-F with SP-guide-R yields the double-stranded DNA molecule SP-guide; annealing SP5G-guide-F with SP5G-guide-R yields the double-stranded DNA molecule SP5G-guide.

[0079] 2) Link the target sequence to the intermediate vector (1) Prepare the intermediate vector digestion system in Table 1, react at 37°C for 2 hours to obtain the linearized vector UBS51; prepare the intermediate vector digestion system in Table 2, react at 37°C for 2 hours to obtain the linearized vector UBS61.

[0080] Table 1. Enzyme digestion system of UBS51 intermediate vector

[0081] Table 2. UBS61 intermediate vector enzyme digestion system

[0082] (2) Prepare the intermediate carrier connection system in Table 3 and react at 25°C for 30 minutes to obtain intermediate carriers UBS51-SlGA20ox1-guide1, UBS51-SlGA20ox2-guide1 or UBS51-SP-guide; prepare the intermediate carrier connection system in Table 4 and react at 25°C for 30 minutes to obtain intermediate carriers UBS61-SlGA20ox1-guide2, UBS61-SlGA20ox2-guide2 or UBS61-SP5G-guide.

[0083] Table 3. Intermediate Carrier Connection System of UBS51-guide1

[0084] Table 4. Intermediate Carrier Connection System of UBS61-guide2

[0085] 3. Connect to the final carrier Prepare the Golden Gate reaction system shown in Table 5 and perform the Golden Gate reaction to ligate the series of intermediate vectors containing gRNA expression cassettes, Cas9 expression cassettes, and NPTII expression cassettes to the final vector. The reaction conditions were 37℃ for 3 min, 16℃ for 4 min, for 20 cycles, followed by 50℃ for 5 min and 80℃ for 10 min. The recombinant vector with the correct sequence obtained from UBS51-SlGA20ox1-guide1 and UBS61-SlGA20ox1-guide2 is designated pAGM4723-ox1. This vector can express Cas9 protein and transcribe sgRNA targeting the SlGA20ox1 gene. The recombinant vector with the correct sequence obtained from UBS51-SlGA20ox2-guide1 and UBS61-SlGA20ox2-guide2 is designated pAGM4723-ox2. This vector can express Cas9 protein and transcribe sgRNA targeting the SlGA20ox2 gene. The recombinant vector with the correct sequence obtained from UBS51-SP-guide and UBS61-SP5G-guide is designated pAGM4723-SP-SP5G. This vector can express Cas9 protein and transcribe sgRNA targeting the SP gene and sgRNA targeting the SP5G gene.

[0086] Table 5. Golden Gate Reaction System

[0087] In the above reaction system, vectors UBS51 and UBS61 were modified from plasmids pICH47751 and pICH47761, respectively. The modification method involved replacing the fragment between the two BpiI restriction sites in pICH47751 and pICH47761 with the DNA molecule shown in SEQ ID No. 13, while keeping the other nucleotides of pICH47751 and pICH47761 unchanged. pICH47751 and pICH47761 are publicly available in the addgene database (https: / / www.addgene.org / ), and their search numbers are shown in Table 6. Vectors pICH47732::NOSp-NPTII-OCST, pICH47742::2x35S-5'UTR-hCas9(STOP)-NOST, pICH41780, and pAGM4723 are also publicly available in the addgene database, and their search numbers are shown in Table 6.

[0088] Table 6. Addgene lookup numbers for vectors

[0089] II. Preparation of Tomato Positive Edited Seedlings The pAGM4723-ox1, pAGM4723-ox2, and pAGM4723-SP-SP5G samples from step one were introduced into the AGL1 Agrobacterium rhizogenes strain to obtain recombinant Agrobacterium AGL1 / pAGM4723-ox1, AGL1 / pAGM4723-ox2, and AGL1 / pAGM4723-SP-SP5G. The resulting recombinant Agrobacterium were then used for genetic transformation of tomato using the leaf disc method. The specific steps are as follows: (1) Plant Ailsa Craig tomato seeds one week in advance. Soak the seeds in sterile water for 30 minutes, disinfect the seed surface with 70% ethanol solution for 2 minutes, disinfect with 10% sodium hypochlorite for 10 minutes, rinse with sterile water 3-4 times, and spot the seeds in 1 / 2 MS medium (1L of medium contains 2.2g MS, 30% (w / w) sucrose, and 0.7% (w / w) agar powder). Treat in the dark for 2 days, and then transfer the medium to light conditions.

[0090] (2) Cut the leaves of tomato seeds that have grown for one week into 0.5cm×0.5cm pieces and place them as explants on the surface of MSO solid medium (1L of medium contains 4.5g MS, 3% (mass percentage) sucrose, 0.7% (mass percentage) agar powder, 0.2mg / L 2,4-D, and 0.1mg / L KT) with a layer of filter paper on the surface. Place the explants with the abaxial end facing down and incubate in the dark at 19℃ for 2 days.

[0091] (3) Inoculate the recombinant Agrobacterium into 3 mL of LB liquid medium containing kanamycin and rifampicin resistance overnight. The next day, inoculate at a ratio of 1:100, shake until the OD600 is 0.6-0.8, collect the cells at 5000 g for 10 min, and resuspend in MSO liquid medium (1 L of medium contains 4.5 g MS, 3% (w / w) sucrose, 0.2 mg / L 2,4-D, 0.1 mg / L KT, and 200 μM acetylsyl syringone) to obtain the MSO resuspension.

[0092] (4) Transfer the leaves from the culture medium in (2) into the MSO resuspension in (3), and shake the resuspension at a constant speed to infect the leaves for 10-15 minutes.

[0093] (5) After infection, pour out the bacterial solution and place the infected leaves on the surface of D1 medium (1L medium contains 4.5g MS, 3% (w / w) sucrose, 0.7% (w / w) agar powder, 0.2mg / L 2,4-D, 0.1mg / L KT, 2mg / L zeatin, and 200μM acetylsylgenone) with filter paper in the dark, with the abaxial end of the explant facing down.

[0094] (6) After step (5) is completed, the leaves are transferred to 2Z medium (1L medium contains 4.5g MS, 3% (w / w) sucrose, 0.7% (w / w) agar powder, 2mg / L zeatin, 200mg / L termethin, 75mg / L kanamycin, 500μM ascorbic acid) to induce resistant callus. The medium is changed every two weeks. After the resistant tissue differentiates into shoots, the callus is transferred to 1Z medium (1L medium contains 4.5g MS, 3% (w / w) sucrose, 0.7% (w / w) agar powder, 1mg / L zeatin, 200mg / L termethin, 75mg / L kanamycin, 500μM ascorbic acid). When the resistant shoots grow to about 2 cm, all explants and callus tissues except the shoots can be removed. The resistant shoots are then transferred to MSSV rooting medium (1 L of medium contains 4.5 g MS, 3% (w / w) sucrose, 0.7% (w / w) agar powder, 0.1 mg / L IAA, 2 mg / L termethin, 50 mg / L kanamycin, and 500 μM ascorbic acid) until the plants root.

[0095] (7) When the rooted seedlings grow to 5cm, extract DNA from the leaves for gene editing identification. Transplant the positive seedlings obtained from the identification. The positive seedlings obtained from AGL1 / pAGM4723-ox1, AGL1 / pAGM4723-ox2, and AGL1 / pAGM4723-SP-SP5G are respectively denoted as...slga20ox1 mutants slga20ox2 mutants sp sp5g Mutant.

[0096] (8) DNA was extracted from tissue culture seedling leaves using the CTAB method and used as a template for PCR amplification and identification. Cas9-F and Cas9-R were used to identify positive seedlings. SlGA20ox1-F and SlGA20ox1-R were used to identify the editing status of the SlGA20ox1 gene target. SlGA20ox2-F and SlGA20ox2-R were used to identify the editing status of the SlGA20ox1 gene target. SP-F and SP-R were used to identify the editing status of the SP gene target. SP5G-F and SP5G-R were used to identify the editing status of the SP5G gene target. Sanger sequencing was used to determine the loss-of-function sequence after gene editing. Primer sequences are as follows: Cas9-F: CGCACAATCCCACTATCCTT; Cas9-R: CTTTTCATGGTACGCCACCT; SlGA20ox1-F: TGGCTATTGATTGTATGATCAC; SlGA20ox1-R:GTATCCATATAACGATGAGC; SlGA20ox2-F:TAAGCGTGAATCTAGTCTGG; SlGA20ox2-R:GTTGGATAATAGTTGAGTCTC; SP-F:ACCAAAAAAACAACTACAACTC; SP-R:GGACAGACAGACGGAGTAAA; SP5G-F: TGATCATTCACAAGATGTG; SP5G-R:TTGGAGCATCAGGATCCACC.

[0097] slga20ox1 The sequence changes of the SlGA20ox1 gene after editing in the mutant are as follows: Figure 1 As shown, compared to tomatoes (AilsaCraig), slga20ox1 The mutant lacks ATTCA at positions 208-212 of SEQ ID No. 1.

[0098] slga20ox2 The sequence changes of the SlGA20ox2 gene after editing in the mutant are as follows: Figure 1 As shown, compared to tomatoes (AilsaCraig), slga20ox2The mutant inserts an A between positions 434 and 435 of SEQ ID No. 4 and a T between positions 986 and 987 of SEQ ID No. 4.

[0099] sp sp5g In mutants SP Genes and SP5G Sequence changes after gene editing, such as Figure 1 As shown, compared to tomatoes (AilsaCraig), sp sp5g mutant SP The TGT gene is deleted from positions 1108-1110 of SEQ ID No. 7. SP5G Gene Deletion SEQ ID GTTTACAACAATAGGGTGGTCTATAATGGATGTTCCTTGAGGCCTTCACAAGTTGTCAATCAACCTAGGGTTGACATTGATGGAGACGATCTTCGTACTTTTTACACTC at positions 1082-1316 of No.10 TGGTATAAACTCATCGTTTTATTTCATATGATATACATATATATATATATATATATATATATATATATTTTCTTTCTATTTATACATTTTAATATCTCTAAATTATTAACCTTTTGTCAA.

[0100] slga20ox1 mutants and sp sp5g Mutants are hybridized and then self-crossed to obtain homozygous mutants. sp sp5g slga20ox1 mutant, slga20ox2 mutants and sp sp5g Mutants are hybridized and then self-crossed to obtain homozygous mutants. sp sp5g slga20ox2 Mutant.

[0101] III. Plant Phenotypic Statistics and Detection The plant to be tested: Wild-type tomato Ailsa Craig (WT). sp sp5g mutant, sp sp5g slga20ox1 mutant, sp sp5g slga20ox2 Mutant.

[0102] 1. Plant Factory Tomato Cultivation and Management Methods The plants to be tested were grown in a plant factory. The plant factory environment was set at 22-26℃, with a photoperiod of 16h light / 8h darkness and a relative humidity of 40%-60%. The unit space of the plant factory was set at 1.2m×0.9m×1m (length×width×height).

[0103] (1) After the seeds germinate, the seedlings with two cotyledons unfolded are transplanted to the hydroponic module of the plant factory at a density of 18 seedlings per unit space and the EC value of the nutrient solution is 0.5-1.0 mS / cm.

[0104] (2) After the plant grows true leaves and before flowering, the EC value of the nutrient solution should be adjusted to 0.8-1.2 mS / cm.

[0105] (3) After the plant flowers, adjust the EC value of the nutrient solution to 1.5-2.0 mS / cm, remove the side branches every 7 days, and use an electric toothbrush to manually assist pollination every 3 days.

[0106] (4) After the plant sets fruit, the EC value of the nutrient solution should be 1.5-2.0 mS / cm. When the leaves are too long and block each other, cut off the first half of the leaf to ensure ventilation and photosynthesis of the whole plant.

[0107] 2. Statistical analysis and detection of plant production efficiency phenotypes Phenotypic testing is conducted in plant factories, and unless otherwise specified, the testing time is 81 days after transplanting.

[0108] (1) Stem length statistics When more than 50% of the flowers in the first inflorescence were open, the length of the primary stem (the stem segment from the first true leaf to the base of the first inflorescence) of each plant was measured. When more than 50% of the flowers in the second inflorescence were open, the length of the sympodial stem between the base of the second and first inflorescences was measured. When more than 50% of the flowers in the third inflorescence were open, the length of the sympodial stem between the base of the third and second inflorescences was measured. When more than 50% of the flowers in the fourth inflorescence were open, the length of the sympodial stem between the base of the fourth and third inflorescences was measured. The average value was recorded as the sympodial stem length of the plant. The p-value was calculated using one-way ANOVA and the Dunnet test for multiple comparisons.

[0109] The results are as follows Figure 2 The AD display shows that, compared to WT, sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 The stem length was significantly shortened. sp sp5g slga20ox1 The stem is the shortest.

[0110] (2) Statistics on ear height The vertical distance between the stem connection point of the first four fruit clusters of each plant (i.e., the base of the lateral branches of the fruit cluster) and the base of the plant outside the hydroponic module (i.e., the lowest point of the visible part of the plant) was measured. The average of the first four fruit clusters was taken as the fruit cluster height of that plant. The p-value was calculated using one-way ANOVA and Dunnet test multiple comparisons.

[0111] The results are as follows Figure 2According to the Chinese E-display, compared to WT, sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 The height of the fruit bunches was significantly reduced. sp sp5g slga20ox1 The ear of fruit is the shortest.

[0112] (3) Leaf length statistics The length of the first leaf below the first inflorescence of each plant was measured, and the p-value was calculated using one-way ANOVA and Dunnet test multiple comparisons.

[0113] The results are as follows Figure 2 The F-value shows that, compared to WT, sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 The leaf length was significantly reduced. sp sp5g slga20ox1 The leaf length is the shortest.

[0114] (4) Statistics on canopy width The diameter of the widest point in the horizontal direction of each plant (i.e., the canopy width) was measured, and the p-value was calculated using one-way ANOVA and Dunnet test multiple comparisons.

[0115] The results are as follows Figure 2 According to G, compared to WT, sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 The canopy width was significantly reduced. sp sp5g slga20ox1 The canopy width is the shortest.

[0116] (5) Statistics on space occupied volume Measure the height (H) of the mature fruit at the very top of each plant, and calculate the space occupied (S) using the canopy diameter (W, i.e., the canopy width in step (4)) and the height (H) of the mature fruit. The calculation formula is S = 3.14(W / 2). 2 ×H. P-values ​​were calculated using one-way ANOVA and Dunnet test multiple comparisons.

[0117] The results are as follows Figure 2 The H-value shows that, compared to WT, sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 The space occupied is significantly reduced. sp sp5g slga20ox1 It occupies the smallest volume of space.

[0118] (6) Statistics on the number of days it takes for fruit to ripen During the plant growth process, the number of days it took for the first fruit of each plant to mature was recorded, and the p-value was calculated using one-way ANOVA and Dunnet test multiple comparisons.

[0119] The results are as follows Figure 2 The data from I shows that, compared to WT, sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 The maturation rate is significantly accelerated. sp sp5g slga20ox1 It matures the fastest.

[0120] (7) Statistics on the number and yield of fruits per plant The number of fruits per plant was counted, and the p-value was calculated using one-way ANOVA and Dunnettest multiple comparisons.

[0121] The results are as follows Figure 2 The J-series data shows that, compared to WT, sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 The number of fruits increased significantly. sp sp5g slga20ox1 The fruit quantity is the largest.

[0122] (8) Statistics on the yield of mature fruits per plant The weight of mature fruit from each plant was counted, and the p-value was calculated using one-way ANOVA and Dunnettest multiple comparisons.

[0123] The results are as follows Figure 2 The K-line shows that, compared to the WT, sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 The fruit yield has increased significantly. sp sp5g slga20ox1 The highest yield is achieved when the fruit is mature.

[0124] (9) Management work hours statistics During the plant growth process, the time spent removing lateral branches and pruning leaves for each line was recorded, and the significance was tested using a t-test.

[0125] The results are as follows Figure 2 The L-shaped display shows that, compared to WT, sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 The required management time is significantly reduced. sp sp5g slga20ox1 It requires the least amount of management time.

[0126] Based on a comprehensive evaluation of all the above phenotypes, considering space cost, time cost, labor cost, and output, sp sp5g , sp sp5g slga20ox1 , sp sp5g slga20ox2 Production efficiency is significantly improved in plant factories, among which sp sp5g slga20ox1 Highest production efficiency 。 This indicates that, through the gibberellin molecular pathway ( SlGA20ox1 andSlGA20ox2 ) and the florigen molecular pathway ( SP and SP5G Molecular polymerization can create short, compact, densely planted, energy-saving, and labor-saving early-maturing, high-yielding, and high-quality tomatoes, significantly improving the production efficiency of tomatoes in plant factories.

[0127] Example 2: Selection of Gibberellin Pathway Genes Within the gibberellin pathway, molecular pathways that can regulate the level of active gibberellins in plants include, but are not limited to, the gibberellin biosynthesis pathway and downstream signal transduction pathways.

[0128] Key enzymes in the gibberellin biosynthesis pathway in tomatoes include, but are not limited to: GA-20 oxidase (GA20ox), GA-2 oxidase (GA2ox), GA-3 oxidase (GA3ox), ent-copalyl diphosphate synthase (CPS), ent-kaurene synthase (KS), ent-kaurenoic acid oxidase (KAO), and ent-kaurene oxidase (KO).

[0129] In tomatoes, key components of the gibberellin signaling pathway include, but are not limited to, the genes encoding gibberellin receptors GID1a, GID1b1, and GID1b2, the related E3 ubiquitin ligase SLY1, and the tomato DELLA protein PROCERA.

[0130] Genes in tomatoes that can be used to edit the gibberellin pathway and reduce the level of active gibberellin include, but are not limited to, genes selected from Table 7 below.

[0131] Table 7. Genes related to the gibberellin pathway in tomatoes

[0132]

[0133]

[0134] SlGA20ox1 and SlGA20ox2 It is expressed in the vegetative meristems, stems, and leaves of tomatoes. The results of Example 1 show that the polymer is expressed. SP SP5G SlGA20ox1 and SP SP5G SlGA20ox2 The tomato mutants with gene modules exhibited varying degrees of stem and leaf shortening and more compact plant growth, resulting in higher adaptability to plant factories and higher production efficiency. This is based on the gene expression patterns in tomatoes as shown in Table 7 of the TomatoeFP Browser database.Figure 3 (https: / / bar.utoronto.ca / efp_tomato / cgi-bin / efpWeb.cgi) utilizes gibberellin pathway genes expressed in vegetative meristems, stems, or leaves. SlGA3ox1, SlGA3ox2, SlGA3ox3, SlGA3ox4, SlGA20ox3, SlGA20ox4, SlGA20ox7, SlGA2ox2, SlGA2ox6, SlGA2ox7, SlGA2ox8, GIB-1, SlCPS-like, GIB-3, GIB-2, SlKAO-like3, SlKO, GID1a, GID1b1, GID1b2, PRO / SlDELLA, SLY1 This can also reduce gibberellin levels in tomatoes and result in a more compact plant type, suggesting that editing or mutagenesis of one or more genes may have been performed. SP SP5G Molecular polymerization via the florigen pathway can achieve results similar to those of the gene module in Example 1, improving the production efficiency of tomatoes in plant factories.

[0135] The present invention has been described in detail above. For those skilled in the art, the invention can be practiced in a wide range of ways with equivalent parameters, concentrations, and conditions without departing from its spirit and scope, and without requiring unnecessary experiments. Although specific embodiments have been given, it should be understood that further modifications can be made to the invention. In summary, according to the principles of the invention, this application is intended to include any changes, uses, or improvements to the invention, including changes made using conventional techniques known in the art that depart from the scope disclosed herein. Some of the essential features can be applied within the scope of the following appended claims.

Claims

1. At least one of the following applications of a protein related to the florigen molecular pathway or a substance that regulates the content or activity of said florigen molecular pathway-related protein: X1) Regulates plant architecture; X2) Prepare products that regulate plant architecture; X3) Regulates the ripening time of plant fruits; X4) Prepare products that regulate the ripening time of plant fruits; X5) Regulates plant fruit yield; X6) Prepare products that regulate plant fruit yield; X7) Cultivating plants with altered plant shape; X8) Prepare products from plants with altered plant type; X9) Cultivating plants whose fruit ripening time is altered; X10) Prepare products from plants that alter fruit ripening time; X11) Cultivating plants that alter fruit yield; X12) Prepare products from plants that alter fruit yield; The proteins involved in the florigen molecular pathway are SP proteins and / or SP5G proteins, wherein the SP proteins are as follows: A1), A2), or A3). A1) The amino acid sequence of this protein is SEQ ID No. 9; A2) A protein with the same function as the amino acid sequence shown in SEQ ID No. 9 in the sequence listing, but with amino acid residue substitution and / or deletion and / or addition; A3) A fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of A1) or A2); The SP5G protein is either B1), B2), or B3). B1) The amino acid sequence of this protein is that of SEQ ID No. 12; B2) A protein with the same function as the amino acid sequence shown in SEQ ID No. 12 in the sequence listing, but with amino acid residue substitution and / or deletion and / or addition; B3) A fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of A1) or A2).

2. The application according to claim 1, characterized in that: The substance that regulates the content or activity of proteins related to the florigen molecular pathway is a substance that regulates the content or activity of the SP protein and / or a substance that regulates the content or activity of the SP5G protein. The substance that regulates the content or activity of the SP protein is any one of the following C1) to C9): C1) The nucleic acid molecule encoding the SP protein; C2) An expression cassette containing the nucleic acid molecule described in C1); C3) A recombinant vector containing the nucleic acid molecule described in C1), or a recombinant vector containing the expression cassette described in C2); C4) Recombinant microorganisms containing the nucleic acid molecules described in C1), or recombinant microorganisms containing the expression cassette described in C2), or recombinant microorganisms containing the recombinant vector described in C3); C5) A transgenic plant cell line containing the nucleic acid molecule described in C1), or a transgenic plant cell line containing the expression cassette described in C2); C6) Transgenic plant tissue containing the nucleic acid molecules described in C1), or transgenic plant tissue containing the expression cassette described in C2); C7) A transgenic plant organ containing the nucleic acid molecule described in C1), or a transgenic plant organ containing the expression cassette described in C2); C8) Nucleic acid molecules that reduce the content or activity of the SP protein; C9) Expression cassettes, recombinant vectors, recombinant microorganisms, transgenic plant cell lines, transgenic plant tissues, or transgenic plant organs containing the nucleic acid molecules described in C8); The substance that regulates the content or activity of the SP5G protein is any one of the following D1) to D9): D1) The nucleic acid molecule encoding the SP5G protein; D2) An expression cassette containing the nucleic acid molecules described in D1); D3) A recombinant vector containing the nucleic acid molecule described in D1), or a recombinant vector containing the expression cassette described in D2); D4) Recombinant microorganisms containing the nucleic acid molecules described in D1), or recombinant microorganisms containing the expression cassette described in D2), or recombinant microorganisms containing the recombinant vector described in D3); D5) A transgenic plant cell line containing the nucleic acid molecule described in D1), or a transgenic plant cell line containing the expression cassette described in D2); D6) Transgenic plant tissue containing the nucleic acid molecules described in D1), or transgenic plant tissue containing the expression cassette described in D2); D7) Transgenic plant organs containing the nucleic acid molecules described in D1), or transgenic plant organs containing the expression cassette described in D2); D8) Nucleic acid molecules that reduce the content or activity of the SP5G protein; D9) Expression cassettes, recombinant vectors, recombinant microorganisms, transgenic plant cell lines, transgenic plant tissues, or transgenic plant organs containing the nucleic acid molecules described in D8).

3. The application according to claim 2, characterized in that: C1) The nucleic acid molecule described is a DNA molecule whose coding sequence is SEQ ID No. 8; D1) The nucleic acid molecule described is a DNA molecule whose coding sequence is SEQ ID No.

11.

4. The application according to any one of claims 1-3, characterized in that: The substance that regulates the content or activity of proteins related to the florigen molecular pathway is a substance that reduces the content or activity of proteins related to the florigen molecular pathway; the regulation of plant form is to reduce the plant form; the regulation of fruit ripening time is to shorten the fruit ripening time; the regulation of fruit yield is to increase the fruit yield; the cultivation of plants with altered plant form is to cultivate plants with reduced plant form; the cultivation of plants with altered fruit ripening time is to cultivate plants with shortened fruit ripening time; and the cultivation of plants with altered fruit yield is to cultivate plants with increased fruit yield.

5. An application of at least one of the following X1)-X12) of a composition: X1) Regulates plant architecture; X2) Prepare products that regulate plant architecture; X3) Regulates the ripening time of plant fruits; X4) Prepare products that regulate the ripening time of plant fruits; X5) Regulates plant fruit yield; X6) Prepare products that regulate plant fruit yield; X7) Cultivating plants with altered plant shape; X8) Prepare products from plants with altered plant type; X9) Cultivating plants whose fruit ripening time is altered; X10) Prepare products from plants that alter fruit ripening time; X11) Cultivating plants that alter fruit yield; X12) Prepare products from plants that alter fruit yield; The composition consists of P1) and P2): P1) The floridin molecular pathway-related protein as described in claim 1 or any of the substances described in claims 1-3 that regulate the content or activity of the floridin molecular pathway-related protein; P2) Gibberellin molecular pathway-related proteins or substances that regulate the content or activity of said gibberellin molecular pathway-related proteins.

6. The application according to claim 5, characterized in that: The gibberellin molecular pathway-related proteins are gibberellin biosynthesis pathway-related proteins and / or gibberellin signal transduction pathway-related proteins.

7. The application according to claim 6, characterized in that: The proteins involved in the gibberellin biosynthesis pathway are GA-20 oxidase, GA-2 oxidase, GA-3 oxidase, Cuban pyrophosphate synthase, endo-kaurene synthase, endo-kaurene oxidase and / or endo-kaurene oxidase. The proteins involved in the gibberellin signal transduction pathway are gibberellin receptors GID1a, GID1b1 and / or GID1b2, E3 ubiquitin ligase SLY1 and / or tomato DELLA protein PROCERA.

8. The application according to claim 7, characterized in that: The GA-20 oxidase is SlGA20ox1 protein and / or SlGA20ox2 protein, and the substance that regulates the content or activity of the gibberellin molecular pathway-related proteins is a substance that regulates the content or activity of SlGA20ox1 protein and / or a substance that regulates the content or activity of SlGA20ox2 protein. The SlGA20ox1 protein is as follows: (E1), (E2), or (E3) E1) The amino acid sequence of this protein is that of SEQ ID No. 3; E2) A protein with the same function as the amino acid sequence shown in SEQ ID No. 3 in the sequence listing, but with the substitution and / or deletion and / or addition of amino acid residues; E3) is a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of E1) or E2); The SlGA20ox2 protein is either G1), G2), or G3). G1) The amino acid sequence of this protein is that of SEQ ID No. 6; G2) A protein with the same function obtained by substituting and / or deleting and / or adding amino acid residues of the amino acid sequence shown in SEQ ID No. 6 in the sequence listing; G3) is a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of G1) or G2); The substance that regulates the content or activity of the SlGA20ox1 protein is any one of the following (H1) to (H9): H1) The nucleic acid molecule encoding the SlGA20ox1 protein; H2) contains an expression cassette containing the nucleic acid molecule described in H1); H3) a recombinant vector containing the nucleic acid molecule described in H1), or a recombinant vector containing the expression cassette described in H2); H4) Recombinant microorganisms containing the nucleic acid molecules described in H1), or recombinant microorganisms containing the expression cassette described in H2), or recombinant microorganisms containing the recombinant vector described in H3); H5) A transgenic plant cell line containing the nucleic acid molecule described in H1), or a transgenic plant cell line containing the expression cassette described in H2); H6) Transgenic plant tissue containing the nucleic acid molecules described in H1), or transgenic plant tissue containing the expression cassette described in H2); H7) Transgenic plant organs containing the nucleic acid molecules described in H1), or transgenic plant organs containing the expression cassette described in H2; H8) Nucleic acid molecules that reduce the content or activity of the SlGA20ox1 protein; H9) Expression cassettes, recombinant vectors, recombinant microorganisms, transgenic plant cell lines, transgenic plant tissues or transgenic plant organs containing the nucleic acid molecules described in H8); The substance that regulates the content or activity of the SlGA20ox2 protein is any one of the following (I1) to (I9): I1) The nucleic acid molecule encoding the SlGA20ox2 protein; I2) An expression cassette containing the nucleic acid molecule described in I1); I3) A recombinant vector containing the nucleic acid molecule described in I1), or a recombinant vector containing the expression cassette described in I2); I4) Recombinant microorganisms containing the nucleic acid molecules described in I1), or recombinant microorganisms containing the expression cassette described in I2), or recombinant microorganisms containing the recombinant vector described in I3); I5) A transgenic plant cell line containing the nucleic acid molecule described in I1), or a transgenic plant cell line containing the expression cassette described in I2); I6) Transgenic plant tissue containing the nucleic acid molecules described in I1), or transgenic plant tissue containing the expression cassette described in I2); I7) Transgenic plant organs containing the nucleic acid molecules described in I1), or transgenic plant organs containing the expression cassette described in I2); I8) Nucleic acid molecules that reduce the content or activity of the SlGA20ox2 protein; I9) Expression cassettes, recombinant vectors, recombinant microorganisms, transgenic plant cell lines, transgenic plant tissues or transgenic plant organs containing the nucleic acid molecules described in I8).

9. The application according to claim 8, characterized in that: H1) The nucleic acid molecule described is a DNA molecule whose coding sequence is SEQ ID No. 2; I1) The nucleic acid molecule described is a DNA molecule with the coding sequence SEQ ID No.

5.

10. The application according to any one of claims 5-9, characterized in that: The substance regulating the content or activity of proteins related to the florigen molecular pathway is a substance that reduces the content or activity of proteins related to the florigen molecular pathway; the substance regulating the content or activity of proteins related to the gibberellin molecular pathway is a substance that reduces the content or activity of proteins related to the gibberellin molecular pathway; the regulation of plant form is to reduce the plant form; the regulation of plant fruit ripening time is to shorten the plant fruit ripening time; the regulation of plant fruit yield is to increase the plant fruit yield; the cultivation of plants with altered plant form is to cultivate plants with reduced plant form; the cultivation of plants with altered fruit ripening time is to cultivate plants with shortened fruit ripening time; and the cultivation of plants with altered fruit yield is to cultivate plants with increased fruit yield.

11. The use of at least one of the following Y1)-Y12) of the SlGA20ox1 protein of claim 8, or the substance of claim 8 or 9 that regulates the content or activity of the SlGA20ox1 protein: Y1) Regulates plant architecture; Y2) Prepare products that regulate plant architecture; Y3) Regulates the ripening time of plant fruits; Y4) Prepare products that regulate the ripening time of plant fruits; Y5) Cultivating plants with altered plant shape; Y6) Prepare products from plants with altered plant type; Y7) Cultivating plants whose fruit ripening time can be altered; Y8) Prepare products from plants that alter the fruit ripening time; Y9) regulates plant fruit yield and at least one of Y1)-Y8); Y10) prepares a product that regulates plant fruit yield and at least one of Y1)-Y8); Y11) Cultivation of plants that alter fruit yield and at least one of Y1)-Y8); Y12) is a product of preparing plants that alter fruit yield and at least one of Y1)-Y8).

12. The application according to claim 11, characterized in that: The substance that regulates the content or activity of the SlGA20ox2 protein is a substance that reduces the content or activity of the SlGA20ox2 protein; the substance that regulates plant type is a substance that reduces plant type; the substance that regulates fruit ripening time is a substance that shortens fruit ripening time; the substance that regulates fruit yield is a substance that increases fruit yield; the substance that cultivates plants with altered plant type is a plant with a reduced plant type; the substance that cultivates plants with altered fruit ripening time is a plant with a shortened fruit ripening time; and the substance that cultivates plants with altered fruit yield is a plant with increased fruit yield.

13. The use of at least one of the following X1)-X12) of the SlGA20ox2 protein of claim 8, or the substance of claim 8 or 9 that regulates the content or activity of the SlGA20ox2 protein: X1) Regulates plant architecture; X2) Prepare products that regulate plant architecture; X3) Regulates the ripening time of plant fruits; X4) Prepare products that regulate the ripening time of plant fruits; X5) Regulates plant fruit yield; X6) Prepare products that regulate plant fruit yield; X7) Cultivating plants with altered plant shape; X8) Prepare products from plants with altered plant type; X9) Cultivating plants whose fruit ripening time is altered; X10) Prepare products from plants that alter fruit ripening time; X11) Cultivating plants that alter fruit yield; X12) is used to prepare products from plants that produce altered fruit yields.

14. The application according to claim 13, characterized in that: The substance that regulates the content or activity of the SlGA20ox2 protein is a substance that reduces the content or activity of the SlGA20ox2 protein; the substance that regulates plant type is a substance that reduces plant type; the substance that regulates fruit ripening time is a substance that shortens fruit ripening time; the substance that regulates fruit yield is a substance that increases fruit yield; the substance that cultivates plants with altered plant type is a plant with a reduced plant type; the substance that cultivates plants with altered fruit ripening time is a plant with a shortened fruit ripening time; and the substance that cultivates plants with altered fruit yield is a plant with increased fruit yield.

15. The application according to any one of claims 1-14, characterized in that: The plant type is reflected in stem length, ear height, leaf size, canopy height and / or plant volume.

16. The application according to any one of claims 1-15, characterized in that: The plant in question is a plant that grows in a confined space.

17. The application according to claim 16, characterized in that: The limited space is a plant factory or greenhouse.

18. The application according to any one of claims 1-17, characterized in that: The plant is any one of M1)-M6): M1) Angiosperms; M2) Dicotyledons; M3) Solanales plants; M4) Solanaceae plants; M5) Solanaceae plants; M6 tomatoes.

19. Any of the following methods: Z1) Methods to reduce plant size, shorten fruit ripening time, and / or increase fruit yield, including: The content or activity of the protein related to the florigen molecular pathway as described in claim 1 in plants is reduced, or the gene encoding the protein related to the florigen molecular pathway as described in claim 1 in plants is knocked out, or the expression level of the gene encoding the protein related to the florigen molecular pathway as described in claim 1 in plants is reduced, thereby achieving a reduction in plant size, a shortening of fruit ripening time, and / or an increase in fruit yield. Z2) A method for cultivating plants with reduced plant size, shortened fruit ripening time, and / or increased fruit yield, comprising: reducing the content or activity of the protein related to the florigen molecular pathway as described in claim 1 in the plant, or knocking out the coding gene of the protein related to the florigen molecular pathway as described in claim 1 in the plant, or reducing the expression level of the coding gene of the protein related to the florigen molecular pathway as described in claim 1 in the plant, to obtain the target plant with reduced plant size, shortened fruit ripening time, and / or increased fruit yield; Z3) A method for reducing plant size, shortening fruit ripening time, and / or increasing fruit yield, comprising: reducing the content or activity of the floridin molecular pathway-related protein as described in claim 1 and the gibberellin molecular pathway-related protein as described in any of claims 5-8 in the plant, or knocking out the coding gene of the floridin molecular pathway-related protein as described in claim 1 and the gibberellin molecular pathway-related protein as described in any of claims 5-8 in the plant, or reducing the expression level of the coding gene of the floridin molecular pathway-related protein as described in claim 1 and the gibberellin molecular pathway-related protein as described in any of claims 5-8 in the plant, thereby achieving a reduction in plant size, a shortening of fruit ripening time, and / or an increase in fruit yield; Z4) A method for cultivating plants with reduced plant size, shortened fruit ripening time, and / or increased fruit yield, comprising: reducing the content or activity of the floridin molecular pathway-related protein as described in claim 1 and the gibberellin molecular pathway-related protein as described in any of claims 5-8 in the plant, or knocking out the coding gene of the floridin molecular pathway-related protein as described in claim 1 and the gibberellin molecular pathway-related protein as described in any of claims 5-8 in the plant, or reducing the expression level of the coding gene of the floridin molecular pathway-related protein as described in claim 1 and the gibberellin molecular pathway-related protein as described in any of claims 5-8 in the plant, thereby obtaining the target plant with reduced plant size, shortened fruit ripening time, and / or increased fruit yield; Z5) A method for reducing plant size, shortening fruit ripening time and / or increasing fruit yield, comprising: reducing the content or activity of the SlGA20ox1 protein as described in claim 8 in the plant, or knocking out the gene encoding the SlGA20ox1 protein as described in claim 8 in the plant, or reducing the expression level of the gene encoding the SlGA20ox1 protein as described in claim 8 in the plant, thereby reducing plant size, shortening fruit ripening time and / or increasing fruit yield; Z6) A method for cultivating plants with reduced plant size, shortened fruit ripening time, and / or increased fruit yield, comprising: reducing the content or activity of the SlGA20ox1 protein as described in claim 8 in the plant, or knocking out the gene encoding the SlGA20ox1 protein as described in claim 8 in the plant, or reducing the expression level of the gene encoding the SlGA20ox1 protein as described in claim 8 in the plant, to obtain the target plant with reduced plant size, shortened fruit ripening time, and / or increased fruit yield; Z7) A method for reducing plant size, shortening fruit ripening time, and / or increasing fruit yield, comprising: reducing the content or activity of the SlGA20ox2 protein as described in claim 8 in the plant, or knocking out the gene encoding the SlGA20ox2 protein as described in claim 8 in the plant, or reducing the expression level of the gene encoding the SlGA20ox2 protein as described in claim 8 in the plant, thereby reducing plant size, shortening fruit ripening time, and / or increasing fruit yield; Z8) A method for cultivating plants with reduced plant size, shortened fruit ripening time, and / or increased fruit yield, comprising: reducing the content or activity of the SlGA20ox2 protein as described in claim 8 in the plant, or knocking out the gene encoding the SlGA20ox2 protein as described in claim 8 in the plant, or reducing the expression level of the gene encoding the SlGA20ox2 protein as described in claim 8 in the plant, to obtain the target plant with reduced plant size, shortened fruit ripening time, and / or increased fruit yield.

20. The method according to claim 19, characterized in that: The methods described in Z1)-Z8) are achieved by editing the gene encoding the target protein.

21. The method according to claim 20, characterized in that: The editing was performed using the CRISPR / Cas9 method.

22. The method according to any one of claims 19-21, characterized in that: The plant in question is a plant that grows in a confined space.

23. The method according to claim 22, characterized in that: The limited space is a plant factory or greenhouse.

24. The method according to any one of claims 19-23, characterized in that: The plant is any one of M1)-M6): M1) Angiosperms; M2) Dicotyledons; M3) Solanales plants; M4) Solanaceae plants; M5) Solanaceae plants; M6 tomatoes.

25. Any of the following products: R1) The florigen pathway-related protein as described in claim 1, or any of the substances described in claims 1-3 that regulate the content or activity of the florigen pathway-related protein; R2) The composition according to any one of claims 5-9; R3) The SlGA20ox1 protein as described in claim 8, or the substance as described in claim 8 or 9 that regulates the content or activity of the SlGA20ox1 protein; R4) The SlGA20ox2 protein as described in claim 8, or the substance as described in claim 8 or 9 that regulates the content or activity of the SlGA20ox2 protein.

Images