A rapid detection method based on chemical structural characteristics of pesticide compounds

Abstract

The application discloses a rapid detection method based on chemical structure characteristics of pesticide compounds, comprising the following steps: identifying effective components of a to-be-detected pesticide and chemical structures of the effective components; according to the chemical structures of the effective components and classification rules, the effective components are classified into corresponding categories of the effective components; according to mobile phase conditions of the corresponding categories of the effective components, mobile phase proportions and chromatographic instrument parameters are set; and according to the mobile phase conditions, sample analysis is carried out on the effective components of the to-be-detected pesticide preparation, and the obtained chromatographic peaks are compared with standard products, so that qualitative and quantitative quality control of the effective components of the pesticide preparation is completed. Through the establishment of a database of common pesticide effective component compounds and their chemical structures, more than 30 different high-performance liquid chromatography mobile phase condition systems are optimized into five kinds, the frequency of method switching and system balancing is reduced, rapid detection is realized, and the result of verification experiment proves that the result is accurate and reliable.

Description

Technical Field

[0001] This invention relates to the field of pesticide detection technology, and more specifically, to a rapid detection method based on the chemical structural characteristics of pesticide compounds. Background Technology

[0002] In current pesticide quality control, high-performance liquid chromatography (HPLC) is a commonly used technique. Pesticide manufacturers and quality testing institutions need to establish and validate an independent HPLC detection method for each pesticide compound or class of compounds. More than 30 different and incompatible HPLC mobile phase conditions exist for the more than 100 mainstream pesticide technicals and formulations on the market.

[0003] In related technologies, quality control laboratories need to prepare dozens of mobile phases for different pesticides and frequently switch methods and balance systems, which greatly reduces the throughput of testing and cannot meet the needs of rapid quality control of raw materials and finished products on the production line.

[0004] It should be noted that the information disclosed in the background section above is only used to enhance the understanding of the background of this disclosure, and therefore may include information that does not constitute prior art known to those skilled in the art. Summary of the Invention

[0005] The technical objective of this application is to address the above-mentioned shortcomings by providing a rapid detection method based on the chemical structural characteristics of pesticide compounds. This application establishes a database of common pesticide active ingredient compounds and their chemical structures, optimizes more than 30 different high-performance liquid chromatography mobile phase conditions into 5, reduces the frequency of method switching and system balancing, thereby achieving rapid detection, and verification experiments confirm that the results are accurate and reliable.

[0006] To achieve the above objectives, this application provides the following technical solution: According to one aspect of this application, a rapid detection method based on the chemical structural characteristics of pesticide compounds is provided, comprising: S100, Sample Identification: Identify the active ingredient of the pesticide to be tested and the chemical structure of the active ingredient; S200. Sample classification: Based on the chemical structure of the active ingredient and the classification rules, the active ingredient is classified into the category corresponding to the active ingredient. The classification rules are as follows: Category 1: Strongly polar and / or hydrophilic compounds, including compounds containing one or more of the following groups: hydroxyl, carboxyl, and sulfonic acid groups; Category 2: Weakly acidic compounds, including compounds containing carboxylic acid esters and / or phenolic hydroxyl groups; Category 3: Weakly basic compounds, including compounds containing amino and / or tertiary amines; Category 4: Neutral and / or nonpolar compounds, including compounds containing one or more of ester bonds, ether bonds, halogens, and benzene rings, and without significant acidity or basicity; Category 5: Special isomer separation, including compounds requiring high resolution to distinguish cis-trans isomers and enantiomers, where high resolution is defined as a resolution greater than 1.5; S300, Method settings: Set the mobile phase ratio and chromatographic instrument parameters according to the mobile phase conditions corresponding to the category of the active ingredient; The mobile phase conditions include the chromatographic column, mobile phase and ratio, elution method, flow rate, column temperature, pH value, and detector; The mobile phase and its proportions are as follows: Category 1: Potassium dihydrogen phosphate aqueous solution: methanol = 93%-97% : 7%-3%, pH value 3-5; Category 2: Acetonitrile:water = 25%-35%: 75%-65%, wherein the aqueous phase contains 0.15% glacial acetic acid, pH value 2-4; Category 3: Methanol: Acetonitrile: Water = 40%-45%: 30-40%: 30%-15%, wherein the aqueous phase contains 0.1% ammonia and has a pH of 7-9; Category 4: Methanol:acetonitrile:water = 35%-40%:40%-45%:25%-15%, pH 6-7; Category 5: n-Hexane:Isopropanol = 80%-90%: 20%-10%, pH 6-7; S400 Sample Analysis: Based on the mobile phase conditions in S300, sample analysis is performed on the active ingredient of the pesticide formulation to be tested. The obtained chromatographic peaks are compared with the standard to complete the qualitative and quantitative quality control of the active ingredient of the pesticide formulation.

[0007] In some embodiments, the sample classification includes classifying the samples according to category priority, wherein the category priority is: Category 5 > Category 2 > Category 3 or Category 1 > Category 4, and samples are preferentially assigned to the category with the higher priority.

[0008] In some embodiments, compounds classified as Category 1 are one of glyphosate, glufosinate, mancozeb, diquat, glufosinate-ammonium, copper oxychloride, and morpholine guanidine hydrochloride; compounds classified as Category 2 are one of bensulfuron-methyl, gibberellin GA3, chlorfenapyr, azoxystrobin, matrine, chlorantraniliprole, pyraclostrobin, cyhalofop-butyl, clodinafop-propargyl, tricyclazole, mesotrione, isopropachlor, and nicosulfuron; compounds classified as Category 3 are one of difenoconazole, pymetrozine, carbendazim, cyazofamid, acetamiprid, flutriafol, emamectin benzoate, cyprodinil, prochloraz, cyanazin, thiamethoxam, cymoxanil, and cypermethrin. The following compounds are included in Category 4: thiamethoxam, thiamethoxam, thiabendazole, tebuconazole, acetochlor, etoxazole, propiconazole, flusilazole, and hexaconazole; Compounds classified as Category 5 are included in Category 6: abamectin, propiconazole, chlorothalonil, pyraclostrobin, flusilazole, thiram, fipronil, hexaconazole, metalaxyl, methoxyfenozide, spirodiclofen, pyrimethanil, cyazofamid, thiamethoxam, fenitrothion, lufenuron, thidium urate, triazophos, bromuconazole, dimethomorph, isoprocarb, hydroxyadenine, and adenine; Compounds classified as Category 5 are included in Category 6: indoxacarb, quizalofop-p-ethyl, high-chlorpyrifos emulsifiable concentrate, high-chlorpyrifos technical powder, lambda-cyhalothrin, and bifenthrin. In some embodiments, the flow rate is 0.8-1.5 mL / min.

[0009] In some embodiments, the column temperature is 40±2℃.

[0010] In some embodiments, the proportions of the mobile phases are as follows: Category 1: Potassium dihydrogen phosphate aqueous solution: Methanol = 97%:3%, pH 3-5; Category 2: Acetonitrile: Water = 35%:65%, wherein the aqueous phase contains 0.15% glacial acetic acid, pH 2-4; Category 3: Methanol: Acetonitrile: Water = 45%:40%:15%, wherein the aqueous phase contains 0.1% ammonia, pH 7-9; Category 4: Methanol: Acetonitrile: Water = 40%:40%:20%, pH 6-7; Category 5: n-Hexane: Isopropanol = 90%:10%, pH 6-7.

[0011] In some embodiments, the elution method is isocratic elution.

[0012] In some embodiments, the chemical structure includes molecular structure, functional groups, acidity / basicity, and polarity parameters.

[0013] Compared with existing technologies, the advantages and positive effects of this application are as follows: This application establishes a database of common pesticide active ingredient compounds and their chemical structures, and optimizes more than 30 different high performance liquid chromatography mobile phase conditions into 5. The laboratory does not need to frequently change the mobile phase and rebalance the chromatographic column, which can realize rapid and continuous detection of different pesticide samples. The detection throughput is expected to be increased by more than 50%, truly realizing "rapid detection".

[0014] Furthermore, this application conducted extensive verification experiments, and the experimental results confirmed the effectiveness and accuracy of the system. In addition, this system reduces errors caused by human error in configuring the mobile phase, ensures the comparability of test results between different personnel, at different times, and in different laboratories, and improves the reliability of quality control data.

[0015] Furthermore, this classification system covers the vast majority of common pesticide compound structural types, has good universality, and can be widely used in pesticide production, quality control, storage, and market supervision.

[0016] Furthermore, reducing the number of mobile phase conditions in high-performance liquid chromatography lowers procurement and warehousing costs; at the same time, it simplifies operating procedures and training cycles, reducing labor costs. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 The chromatogram of Comparative Example 1 of this application is shown; Figure 2 The chromatogram shown is from Example 1 of this application; Figure 3 The chromatogram of Comparative Example 2 of this application is shown; Figure 4 The chromatogram shown in Embodiment 2 of this application is illustrated. Figure 5 The chromatogram of Comparative Example 3 of this application is shown; Figure 6 The chromatogram of Example 3 of this application is shown; Figure 7 The chromatogram of Comparative Example 4 of this application is shown; Figure 8 The chromatogram of Embodiment 4 of this application is shown; Figure 9 The chromatogram of Comparative Example 5 of this application is shown; Figure 10 The chromatogram of Example 5 of this application is shown. Detailed Implementation

[0019] To better understand the above-mentioned objectives, features, and advantages of this application, the application will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in the embodiments of this application can be combined with each other.

[0020] Step 1: Establish a compound structure-chromatographic behavior database.

[0021] More than 100 pesticide compounds were collected and their molecular structure, functional groups, acidity, basicity, and polarity parameters were analyzed. These parameters were then correlated with their chromatographic behavior (retention time, peak symmetry, and resolution) under different mobile phase conditions in national, industry, and enterprise standards to form a basic database.

[0022] The contents of the basic database, for example: Pesticide compound name: glyphosate Molecular structure: ; Functional group: glycine derivatives; Acidity / alkalinity: 1.4-2.4; Polarity parameter: 107; National Standard GB / T20684-2017: Ultraviolet Spectrophotometry.

[0023] Step 2: Establish structural classification principles Based on the aforementioned database, we established the following classification principles, dividing pesticide compounds into six core categories. Classification was performed according to category priority, which was: Category 5 > Category 2 > Category 3 or Category 1 > Category 4, with higher priority categories being assigned first. Details are as follows:

[0024] The categories are sorted according to their priority, which is either Category 5 > Category 2 > Category 3 or Category 1 > Category 4. Categories with higher priority are assigned to the higher priority category.

[0025] In other words, when classifying, we first determine whether it meets the requirements of the category with the higher priority. If it does, it is directly assigned to the corresponding category. If it does not meet the requirements, we then determine the next level of category, and so on.

[0026] For example: glyphosate: Check category 5: No.

[0027] Category 2: Contains neither carboxylic esters nor phenolic hydroxyl groups.

[0028] Check categories 3 and 1: These are not weakly basic compounds, but rather strongly polar and hydrophilic compounds. They are classified into category 1 and do not require further investigation into subsequent categories.

[0029] Step 3: Match uniform optimized mobile phase conditions for each category: For the five categories mentioned above, we optimized and fixed their HPLC mobile phase conditions through extensive experiments. Taking a reversed-phase C18 column as an example, the detector type was a VWD detector; the wavelength range was 190-260 nm; and the injection volume was 5-10 μL.

[0030] Its core conditions are defined as follows: Category 1: Strongly polar and / or hydrophilic compounds, mobile phase: potassium dihydrogen phosphate aqueous solution: methanol = (93-97)%: (7-3)%, pH value 3-5, flow rate: 0.8-1.5 ml / min, column temperature: 40±2℃.

[0031] Category 2: Weakly acidic compounds, mobile phase: acetonitrile: water (containing 0.15% glacial acetic acid) = (25-35)%: (75-65)%, pH value 2-4, flow rate: 0.8-1.5 mL / min, column temperature: 40±2℃.

[0032] Category 3: Weakly basic compounds, mobile phase: methanol: acetonitrile: water (containing 0.1% ammonia) = (40-45)%: (30-40)%: (30-15)%, pH 7-9, flow rate: 0.8-1.5 mL / min, column temperature: 40±2℃. Wherein, water (containing 0.1% ammonia) is aqueous phase containing 0.1% ammonia.

[0033] Category 4: Neutral and / or non-polar compounds, mobile phase: methanol: acetonitrile: water = (35-40)%: (40-45)%: (25-15)%, pH 6-7, flow rate: 0.8-1.5 mL / min, column temperature: 40±2℃.

[0034] Category 5: Special isomer separation, mobile phase: n-hexane:isopropanol = (80-90)%: (20-10)%, pH 6-7, flow rate: 0.8-1.5 mL / min, column temperature: 40±2℃.

[0035] Step 4: Quality Control Application Process S100 Sample Identification: Identify the chemical structure of the active ingredient in the pesticide formulation to be tested.

[0036] S200. Sample Classification: Based on the classification principles in step two, classify the sample into one of the five categories mentioned above.

[0037] S300, Method Settings: Based on the uniform mobile phase conditions corresponding to this category in step three, set the HPLC instrument parameter ratios.

[0038] S400. Sample Analysis: Sample analysis is performed according to the method in S300 (based on GB / T 16631-2008 General Rules for High Performance Liquid Chromatography). The obtained chromatographic peaks (retention time, peak area) are compared with the standards to complete the qualitative and quantitative quality control of the active ingredients in pesticide formulations. (Calculation basis: GB / T 16631-2008 General Rules for High Performance Liquid Chromatography) It should be noted that this application is specifically for pesticide formulation manufacturers, used for rapid quality inspection of pesticide formulations produced on the production line. By pre-classifying pesticide compounds, corresponding high-performance liquid chromatography (HPLC) mobile phase conditions are identified. The classification system covers the vast majority of common pesticide compound structural types. If a compound cannot be classified into a specific category during pre-classification, it is first classified according to priority, or manual verification can be performed to confirm the applicable mobile phase conditions. Furthermore, pesticide manufacturers have prior knowledge of the active ingredient compounds in their pesticide formulations; therefore, there is no possibility of being unable to classify them based on their chemical structures.

[0039] The present application will be further described below with reference to the accompanying drawings and specific embodiments.

[0040] Comparative Example 1: Existing method for testing 30% glufosinate aqueous solution.

[0041] The active ingredient in 30% glufosinate aqueous solution is glufosinate. Testing was conducted according to GB 33808-2017, and the results are shown below. Figure 1 and Table 1. From Figure 1 As shown in Table 1, the peak retention time is relatively long (6.3 min).

[0042] Table 1 Results of glufosinate detection in Comparative Example 1

[0043] Example 1: Testing of 30% glufosinate aqueous solution (classified as Category 1).

[0044] S100 Sample Identification: The sample to be tested is 30% glufosinate aqueous solution. Its active ingredient, glufosinate, contains amino and carboxyl groups in its molecule, making it a highly polar compound. S200. Sample Classification: According to the classification rules, classify it into Category 1 (strongly polar and / or hydrophilic compounds). S300, Method Settings: Column: Agilent, ZORBAX (4.6×150mm, 5μm); Mobile phase: Potassium dihydrogen phosphate aqueous solution: Methanol = 97%: 3%; pH value: 3-5; Elution method: isocratic elution; Flow rate: 1.0 mL / min; Column temperature: 40℃; Detector: Ultraviolet detector (UV195nm).

[0045] S400, Sample Analysis: Detection results are shown below. Figure 2 and Table 2, as shown Figure 2 As shown in Table 2, using the Category 1 method, the retention time of glufosinate was stable at approximately 3.8 minutes, with good peak shape, complete separation from the adjuvants in the formulation, and accurate and reliable quantitative results.

[0046] Table 2 Results of glufosinate detection in Example 1

[0047] Comparative Example 2: Existing method for detecting nicosulfuron oil suspension.

[0048] The active ingredient, nicosulfuron, in nicosulfuron oil suspension was tested according to GB 29382-2012, and the results are shown below. Figure 3 And Table 3. (For example...) Figure 3 As shown in Table 3, the retention time of the main peak using the original method was 5.4 min, but the separation was poor.

[0049] Table 3 Results of Detection of Mesotrione in Comparative Example 2

[0050] Example 2: Detection of nicosulfuron oil suspension (classified as Category 2).

[0051] S100 Sample Identification: The sample to be tested is nicosulfuron suspension, and its active ingredient, nicosulfuron, has a β-trione structure and is a weakly acidic compound.

[0052] S200, Sample Classification: According to the classification rules, it is classified into category 2 (weak acid compound).

[0053] S300, Method Settings: Chromatographic column: Conventional C18 reversed-phase column (250mm × 4.6mm, 5μm); Mobile phase: Acetonitrile: Water (containing 0.15% glacial acetic acid) = 35%: 65%; pH value: 2-4; Elution method: isocratic elution; Flow rate: 1.0 mL / min; Column temperature: 40℃; Detector: Ultraviolet detector (UV260nm).

[0054] S400, Sample Analysis: Detection results are shown below. Figure 4and Table 4, as shown Figure 4 As shown in Table 4, using the Category 2 method, the main peak retention was 8.7 min, the tailing factor was 1.1, which was a significant improvement. The peak shape was good, and it was completely separated from the excipients in the formulation. The quantitative results were accurate and reliable.

[0055] Table 4 Results of testing nicosulfuron oil suspension in Example 2

[0056] Comparative Example 3: The original method was used to detect 40% phenyl ether·pyraclostrobin suspension.

[0057] The active ingredient in 40% difenoconazole·pyraclostrobin suspension concentrate is difenoconazole. Testing was conducted according to NY / T4385-2023, and the results are shown below. Figure 5 And Table 5. (For example...) Figure 5 As shown in Table 5, the peaks are bimodal, the retention times are (20.5 + 23.6), and the resolution is poor.

[0058] Table 5 Results of detection of difenoconazole in Comparative Example 3

[0059] Example 3: Detection of 40% difenoconazole·pyraclostrobin suspension (classified as Category 3).

[0060] S100, Sample Identification: The sample to be tested is 40% difenoconazole·pyraclostrobin suspension, whose active ingredient difenoconazole contains a triazole ring and is a weakly basic compound.

[0061] S200, Sample Classification: According to the classification rules, it is classified into category 3 (weakly basic compound).

[0062] S300, Method Settings: Chromatographic column: Conventional C18 reversed-phase column (250mm×4.6mm, 5μm); Mobile phase: methanol: acetonitrile: water (containing 0.1% ammonia) = 45%: 40%: 15%; wherein, water (containing 0.1% ammonia) is added to the aqueous phase with 0.1% ammonia solution; pH value: 7-9; Elution method: isocratic elution; Flow rate: 1.0 mL / min; Column temperature: 40℃; Detector: Ultraviolet detector (UV230nm).

[0063] S400, Sample Analysis: Detection results are shown below. Figure 6 and Table 6, as shown Figure 6As shown in Table 6, the retention time of difenoconazole was stable at approximately 13.7 minutes, with good peak shape, and it was completely separated from the excipients in the formulation, resulting in accurate and reliable quantitative results.

[0064] Table 6 Results of detection of difenoconazole in Example 3

[0065] Comparative Example 4: The original method was used to detect 5% avermectin emulsifiable concentrate.

[0066] The active ingredient in avermectin emulsifiable concentrate is avermectin. Testing was conducted according to GB / T19336-2017, and the results are shown below. Figure 7 And Table 7. (For example...) Figure 7 As shown in Table 7, the main peak retention time is relatively long (26.2 min).

[0067] Table 7 Results of Detection of Avermectin Emulsifiable Concentrate in Comparative Example 4

[0068] Example 4: Detection of 5% avermectin emulsifiable concentrate (classified as Category 4) S100 Sample Identification: The sample to be tested is 5% abamectin emulsifiable concentrate. Its active ingredient, abamectin, contains ester bonds and ether bonds and has no significant acidity or alkalinity, making it a neutral compound.

[0069] S200, Sample Classification: According to the classification rules, it is classified into category 4 (neutral and / or weakly polar compounds).

[0070] S300, Method Settings: Chromatographic column: Conventional C18 reversed-phase column (250mm×4.6mm, 5μm); Mobile phase: Methanol: Acetonitrile: Water = 40%: 40%: 20%; pH value: 6-7; Elution method: isocratic elution; Flow rate: 1.0 ml / min; Column temperature: 40℃ Detector: Ultraviolet detector (UV245nm).

[0071] S400, Sample Analysis: Detection results are shown below. Figure 8 and Table 8, as shown Figure 8 As shown in Table 8, using the Category 4 method, the retention time of avermectin was stable at approximately 18.9 minutes, with good peak shape, complete separation from the excipients in the formulation, and accurate and reliable quantitative results.

[0072] Table 8 Results of avermectin detection in Example 4

[0073] Comparative Example 5: Existing method for detecting 12% abamectin·indoxacarb microemulsion.

[0074] The active ingredient in 12% abamectin·indoxacarb microemulsion is indoxacarb. Testing was conducted according to HG / T4933-2016, and the results are shown below. Figure 9 And Table 9. (For example...) Figure 9 As shown in Table 9, the retention time of the main peak is 4.0 min.

[0075] Table 9 Results of indoxacarb detection in Comparative Example 5

[0076] Example 5: Detection of 12% abamectin·indoxacarb microemulsion (classified as category 5).

[0077] S100 Sample Identification: The sample to be tested is 12% abamectin·indoxacarb, whose active ingredient, indoxacarb, is a chiral compound, a mixture of S-form and R-form, with the active form being the S-form.

[0078] S200, Sample Classification: According to the classification rules, it is classified into category 5 (separation of special isomers).

[0079] S300, Method Settings: Chromatographic column: Chiral column OD-H (250mm×4.6mm, 5μm); Mobile phase: n-hexane:isopropanol = 90%:10%; pH value: 6-7; Elution method: isocratic elution; Flow rate: 1.0 mL / min; Column temperature: 40℃; Detector: Ultraviolet detector (UV285nm).

[0080] S400, Sample Analysis: Detection results are shown below. Figure 10 and Table 10, as follows Figure 10 As shown in Table 10, using Category 5 method, the S and R bodies of indoxacarb can be effectively separated. The retention time of the S body is stable at about 10.2 minutes, with good peak shape, and it is completely separated from the adjuvants in the formulation. The quantitative results are accurate and reliable.

[0081] Table 10 Results of indoxacarb detection in Example 5

[0082] It should be noted that the sensitivity, linear range, precision, and accuracy of the above methods all meet the general rules of GB / T 16631-2008 High Performance Liquid Chromatography.

[0083] Through the above specific embodiments, those skilled in the art can easily implement this application. However, it should be understood that this application is not limited to the specific embodiments described above. Based on the disclosed embodiments, those skilled in the art can arbitrarily combine different technical features to achieve different technical solutions.

Claims

1. A rapid detection method based on the chemical structural characteristics of pesticide compounds, characterized in that, include: S100, Sample Identification: Identify the active ingredient of the pesticide to be tested and the chemical structure of the active ingredient; S200. Sample classification: Based on the chemical structure of the active ingredient and the classification rules, the active ingredient is classified into the category corresponding to the active ingredient. The classification rules are as follows: Category 1: Strongly polar and / or hydrophilic compounds, including compounds containing one or more of the following groups: hydroxyl, carboxyl, and sulfonic acid groups; Category 2: Weakly acidic compounds, including compounds containing carboxylic acid esters and / or phenolic hydroxyl groups; Category 3: Weakly basic compounds, including compounds containing amino and / or tertiary amines; Category 4: Neutral and / or nonpolar compounds, including compounds containing one or more of ester bonds, ether bonds, halogens, and benzene rings, and without significant acidity or basicity; Category 5: Special isomer separation, including compounds requiring high resolution to distinguish cis-trans isomers and enantiomers, where high resolution is defined as a resolution greater than 1.5; S300, Method settings: Set the mobile phase ratio and chromatographic instrument parameters according to the mobile phase conditions corresponding to the category of the active ingredient; The mobile phase conditions include the chromatographic column, mobile phase and ratio, elution method, flow rate, column temperature, pH value, and detector; The mobile phase and its proportions are as follows: Category 1: Potassium dihydrogen phosphate aqueous solution: methanol = 93%-97% : 7%-3%, pH value 3-5; Category 2: Acetonitrile:water = 25%-35%:75%-65%, wherein the aqueous phase contains 0.15% glacial acetic acid, and the pH value is 2-4; Category 3: Methanol: Acetonitrile: Water = 40%-45%: 30-40%: 30%-15%, wherein the aqueous phase contains 0.1% ammonia and has a pH of 7-9; Category 4: Methanol:acetonitrile:water = 35%-40%:40%-45%:25%-15%, pH 6-7; Category 5: n-Hexane:Isopropanol = 80%-90%: 20%-10%, pH 6-7; S400 Sample Analysis: Based on the mobile phase conditions in S300, sample analysis is performed on the active ingredient of the pesticide formulation to be tested. The obtained chromatographic peaks are compared with the standard to complete the qualitative and quantitative quality control of the active ingredient of the pesticide formulation.

2. The rapid detection method based on the chemical structural characteristics of pesticide compounds according to claim 1, characterized in that, The sample classification includes: classifying according to category priority, wherein the category priority is: Category 5 > Category 2 > Category 3 or Category 1 > Category 4, and samples are preferentially assigned to the category with the higher priority.

3. The rapid detection method based on the chemical structural characteristics of pesticide compounds according to claim 2, characterized in that, The compounds classified into Category 1 are one of the following: glyphosate, glufosinate, mancozeb, diquat, glufosinate, copper oxychloride, and morpholine guanidine hydrochloride. The compounds classified into Category 2 are one of the following: bensulfuron-methyl, gibberellin GA3, chlorfenapyr, azoxystrobin, matrine, chlorantraniliprole, pyraclostrobin, cyhalofop-butyl, clodinafop-propargyl, tricyclazole, mesotrione, isopropachlor, and nicosulfuron-methyl. The compounds classified into Category 3 are one of the following: difenoconazole, pymetrozine, carbendazim, cyazofamid, acetamiprid, flutriafol, emamectin benzoate, cyprodinil, imazalil, cyanazin, thiamethoxam, cymoxanil, chlorpyrifos, thiamethoxam, thiamethoxam, thiamethoxam, oxadiazon, tebuconazole, acetochlor, etoxazole, propiconazole, flusilazole, and hexaconazole. The compounds classified into Category 4 are one of the following: abamectin, propiconazole, chlorothalonil, pyraclostrobin, flusilazole, thiram, fipronil, hexaconazole, metalaxyl, methoxyfenozide, spirodiclofen, pyrimethanil, cyazofamid, thiamethoxam, fenitrothion, lufenuron, thiabendazole, triazophos, bromuconazole, dimethomorph, isoprocarb-hydroxyadenine, and adenine. The compounds classified into category 5 are one of the following: indoxacarb, quizalofop-p-ethyl, high-chlorpyrifos emulsifiable concentrate, high-chlorpyrifos technical powder, lambda-cyhalothrin, and bifenthrin.

4. The rapid detection method based on the chemical structural characteristics of pesticide compounds according to claim 1, characterized in that, The flow rate is 0.8-1.5 mL / min.

5. The rapid detection method based on the chemical structural characteristics of pesticide compounds according to claim 1, characterized in that, The column temperature is 40±2℃.

6. The rapid detection method based on the chemical structural characteristics of pesticide compounds according to claim 1, characterized in that, The proportions of the mobile phase are as follows: Category 1: Potassium dihydrogen phosphate aqueous solution: methanol = 97%: 3%, pH 3-5; Category 2: Acetonitrile:water = 35%:65%, wherein the aqueous phase contains 0.15% glacial acetic acid, and the pH value is 2-4; Category 3: Methanol:acetonitrile:water = 45%:40%:15%, wherein the aqueous phase contains 0.1% ammonia and has a pH of 7-9; Category 4: Methanol:acetonitrile:water = 40%:40%:20%, pH 6-7; Category 5: n-Hexane:Isopropanol = 90%:10%, pH 6-7.

7. The rapid detection method based on the chemical structural characteristics of pesticide compounds according to claim 1, characterized in that, The elution method is isocratic elution.

8. The rapid detection method based on the chemical structural characteristics of pesticide compounds according to claim 1, characterized in that, The chemical structure includes molecular structure, functional groups, acidity / basicity, and polarity parameters.

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