Chromatographic column device and method for producing the same

Abstract

The application provides a chromatographic column device and a preparation method thereof. The chromatographic column device comprises a first end plate, a second end plate and a chromatographic column layer clamped between the two end plates. The chromatographic column layer comprises a middle plate, a sealing gasket and a partition plate. At least one side surface of the middle plate is forged and formed with a groove. The sealing gasket is attached to the side of the middle plate where the groove is formed. The partition plate is attached to the outside of the sealing gasket. The first end plate, the second end plate, the middle plate, the sealing gasket and the partition plate are tightly connected to extrude the sealing gasket and cause the sealing gasket to deform. The sealing gasket and the groove of the middle plate jointly form a chromatographic column channel. The chromatographic column channel is filled with chromatographic packing. The application solves the industry difficulties of large-size chromatographic column preparation, uncontrollable forming quality, greatly reduces the overall manufacturing process threshold and processing difficulty of the chromatographic column, effectively reduces the preparation and production cost, breaks through the industry technical barriers of traditional chromatographic column preparation, and realizes the simplification, standardization and large-scale popularization and application of chromatographic column preparation.

Description

Technical Field

[0001] This invention relates to the field of chromatographic column preparation technology, and in particular to a chromatographic column apparatus and its preparation method. Background Technology

[0002] The chromatographic column is the core component of chromatographic analysis equipment. Its structural regularity, packing uniformity, and degree of manufacturing standardization directly determine the accuracy, stability, and repeatability of chromatographic detection. Currently, the mainstream chromatographic column structures on the market mainly include three types: long tubular packed columns, microchannel columns, and plate channel columns. However, all existing technologies have significant technical defects, making it difficult to simultaneously meet the requirements of universality, standardization, miniaturization, low cost, and high stability. The specific defects are as follows.

[0003] Traditional large-size packed-filler chromatographic columns mostly adopt a long tubular structure, which has inherent manufacturing defects and performance shortcomings. Firstly, it is difficult to precisely control the uniformity of packing material filling within the long tubular cavity. During preparation, problems such as uneven packing density distribution, internal voids, and layering are easily encountered, directly causing inconsistencies in monomer separation and detection performance between different columns, resulting in extremely poor product consistency. Secondly, the preparation process for this type of column has not yet formed a standardized process that can be completely replicated. The preparation quality is highly dependent on the operator's technical experience, and differences in human operation further exacerbate product performance deviations, making large-scale, standardized mass production impossible. Thirdly, traditional long tubular chromatographic columns require first completing the straight tube packing and then bending it into a coil. During the bending process, the packing material inside the tube undergoes tensile, stacking, and compressive deformation, leading to problems such as packing material breakage and disordered pore structure. Furthermore, these internal structural defects are highly concealed, impossible to observe or repair, significantly reducing the column's performance and lifespan.

[0004] Meanwhile, traditional long tubular chromatographic columns have significant structural and dimensional limitations, resulting in extremely poor spatial adaptability. Their elongated shape makes them unsuitable for efficient layout within confined, regular spaces, leading to significant challenges in equipment integration. Furthermore, the accompanying temperature control system cannot be miniaturized, resulting in a large overall equipment footprint and low integration, hindering upgrades towards lightweight and miniaturized designs. In addition, the preparation process of these columns is highly susceptible to various random factors such as temperature, operating techniques, and packing pressure, leading to significant batch-to-batch variations. This severely restricts the reproducibility and stability of scientific experiments, significantly increasing the workload and costs associated with experimental debugging and calibration.

[0005] Existing microchannel chromatographic columns are severely limited in their applicability and widespread use due to structural material and manufacturing process constraints. These columns are only suitable for specialized, precision equipment at the microscale, resulting in a narrow range of applications. Their overall manufacturing process is complex and cumbersome, requiring extremely high precision in production equipment, advanced hardware configurations, and highly skilled operators. Furthermore, the range of compatible materials is limited, exhibiting poor material versatility. The stringent manufacturing conditions and specific material requirements lead to high production costs, hindering large-scale industrial application and limiting their use to a few specialized experimental scenarios and niche research institutions. They cannot meet the large-scale needs of general chromatographic detection. Simultaneously, their tiny channel structure makes them unsuitable for handling large volumes of packing material, completely negating their compatibility with general-purpose chromatographic columns.

[0006] Existing plate-type channel chromatographic columns also have significant technical shortcomings, resulting in extremely low value for widespread application. These products have not yet overcome the core sealing technology challenges; addressing sealing defects requires complex processing techniques and special components, directly leading to high processing costs. Compared to traditional long tubular chromatographic columns, their cost advantage is completely lost, resulting in extremely low cost-effectiveness. Furthermore, their overall structural design is unreasonable, disassembly and assembly are cumbersome, structural reusability and maintainability are poor, and the cost per use is high, making it impossible to achieve simplified and routine application.

[0007] In summary, current mainstream chromatographic column technologies suffer from numerous drawbacks, including poor performance consistency, low standardization, insufficient spatial integration, complex processes, high costs, limited adaptability, and poor reusability. No single technology can simultaneously meet the industry demands for universal size, standardized manufacturing, low cost, high stability, and miniaturized integration. This severely restricts the widespread application of chromatographic detection technologies and the industrial upgrading of equipment. Therefore, there is an urgent need to develop a novel chromatographic column structure and preparation process to address the various pain points of existing technologies.

[0008] In view of this, the present invention is hereby proposed. Summary of the Invention

[0009] The technical problem to be solved by this invention is to overcome at least some of the shortcomings of the prior art and provide a chromatographic column device. By using a forging process to form the channel, the end plates at both ends, the intermediate plate sandwiched in the middle, the sealing gasket, and the partition plate are connected by a fastening method to compress the sealing gasket, so that the sealing gasket and the channel on the intermediate plate form a chromatographic column channel. This can solve the difficulties in preparing large-sized chromatographic columns, reduce the difficulty of manufacturing, promote the popularization and application of preparation technology, reduce preparation costs, break down technical barriers in the industry, and realize the simplification, standardization, and popularization of chromatographic column preparation.

[0010] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by the present invention is as follows: A chromatographic column apparatus includes a first end plate, a second end plate, and a chromatographic column layer sandwiched between the first end plate and the second end plate; The chromatographic column includes an intermediate plate, a sealing gasket, and a partition. At least one side of the intermediate plate is forged and formed with a groove. The sealing gasket is fitted and covers the groove opening side of the intermediate plate, and the partition covers the outside of the sealing gasket. The first end plate, the second end plate, the intermediate plate, the sealing gasket, and the partition are fastened together to compress the sealing gasket and deform it, so that the sealing gasket and the groove of the intermediate plate together enclose the chromatographic column channel, and the chromatographic column channel is filled with chromatographic packing material.

[0011] In some embodiments, the materials used to prepare the first end plate, the second end plate, the intermediate plate, the sealing gasket, and the partition are selected from any one of metallic materials, organic materials, and modified materials; The material hardness of the sealing gasket is less than that of the materials of the first end plate, the second end plate, the intermediate plate, and the partition.

[0012] In some embodiments, bolt holes are provided on the first end plate, the second end plate, the intermediate plate, the sealing gasket, and the partition plate. By passing a screw through the corresponding bolt holes of each component and applying pressure to both ends of the screw, the first end plate, the second end plate, the intermediate plate, the sealing gasket, and the partition plate are pressed, fixed, and sealed.

[0013] In some embodiments, the intermediate plate and the partition plate are respectively provided with sealing grooves, wherein the sealing grooves on the intermediate plate surround the outside of the channel, and during fastening connection, part of the structure of the sealing gasket is squeezed into the two opposing sealing grooves.

[0014] In some embodiments, the surface of the sealing gasket is coated with a sealing protective layer, which is made of silver or nickel.

[0015] In some embodiments, multiple layers of the chromatographic column are stacked between the first end plate and the second end plate.

[0016] In some implementations, when the opposite sides of the intermediate plates of two adjacent chromatographic column layers are both formed with channels by forging, the opposite channel sides of the two chromatographic column layers share a partition plate.

[0017] In some embodiments, the inner surface of the first end plate and / or the second end plate facing the chromatographic column layer is formed with channels by a forging process; In this embodiment, the groove opening side of the first end plate and / or the second end plate is fitted and covered with a sealing gasket.

[0018] In some embodiments, one of the first end plate and the second end plate is provided with a medium inflow interface, and the other is provided with a medium outflow interface; The intermediate plate, sealing gasket and partition of the chromatographic column are all provided with corresponding air passages, and the air passages are interconnected to form a medium flow path. Preferably, different air vents can be provided on the partition to achieve series connection between different channels.

[0019] The present invention also provides a method for preparing a chromatographic column apparatus, applicable to the chromatographic column apparatus described above, comprising: According to the usage requirements, a first end plate, a second end plate, an intermediate plate, a sealing gasket, and a partition are prepared by forging process, wherein at least one side surface of the intermediate plate is forged and formed with a groove. The first end plate, the middle plate, the sealing gasket, the partition and the second end plate are assembled in sequence by stacking and laying layer by layer. During the assembly process, the chromatographic packing is uniformly filled into the groove of the middle plate, and the sealing gasket and the partition are sequentially attached and laid on the groove opening side of the middle plate. The assembled components are tightened and connected, causing the sealing gasket to deform under pressure and cooperate with the groove of the intermediate plate to form a sealed chromatographic column channel, thus completing the preparation of the chromatographic column device.

[0020] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art.

[0021] (1) The chromatographic column device provided by the present invention adopts a forging process to form an integral plate channel structure. The end plates, middle plate, sealing gasket and partition are pressed and fixed by a fastening assembly method. The sealing gasket and the middle plate channel are matched by compression sealing to form a complete chromatographic column fluid channel. This structural design fundamentally solves the industry difficulties of large-size chromatographic column preparation and uncontrollable molding quality. It significantly reduces the overall manufacturing process threshold and processing difficulty of chromatographic column, effectively reduces the preparation and production cost, breaks the industry technical barrier of traditional chromatographic column preparation, and realizes the simplification, standardization and large-scale popular application of chromatographic column preparation.

[0022] (2) The chromatographic column device provided by the present invention, the core components such as the first end plate, the second end plate, the intermediate plate, the sealing gasket and the partition, can all be made of metal materials (stainless steel, iron, copper, aluminum, etc.) or non-metal materials (organic materials, modified materials, etc.) that are compatible with the forging process. The raw materials are widely selected and the molding process is simple, which can effectively control the mass production cost. At the same time, the present invention can flexibly select partitions and sealing gaskets of different shapes and materials for matching and assembly according to the actual detection and equipment adaptation requirements, which can adapt to various working conditions and equipment usage scenarios. The device has strong expandability and versatility.

[0023] (3) The chromatographic column device provided by the present invention adopts a modular assembly method of layer-by-layer laying and layer-by-layer assembly, which can realize the entire process of visual assembly operation, completely abandoning the traditional long tube filling and bending process mode. This assembly method can accurately ensure the uniformity and consistency of the internal flow channel and packing structure of the chromatographic column, effectively avoiding the problems of void defects, packing extrusion and breakage, and pore disorder in the traditional process, and greatly eliminating various random interference factors in the preparation process. At the same time, the device is easy to disassemble and assemble. If structural or performance problems occur, it can be quickly disassembled for investigation, inspection and rectification, and maintenance is highly convenient. In addition, the present invention significantly reduces the requirements for the technical experience of operators and production equipment, and is suitable for research units and production enterprises with limited technical conditions and imperfect equipment configuration to prepare and use independently, further breaking through the industry technical barriers and facilitating the large-scale promotion of the technology.

[0024] (4) The chromatographic column device provided by the present invention has a simple overall structure, reliable assembly, strong stability, and extremely high design flexibility in structural dimensions. It can be customized to produce chromatographic columns of specific sizes according to different experimental parameters and equipment integration requirements, and can also be standardized to produce general-purpose products to meet the needs of mass sales in the market. It takes into account both customized applications and general-purpose mass production, and is suitable for a wide range of scenarios. Attached Figure Description

[0025] The accompanying drawings, as part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention, but do not constitute an undue limitation of the invention. Obviously, the drawings described below are merely some embodiments, and those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings: Figure 1 This is a partial structural schematic diagram of a chromatography column apparatus provided according to an exemplary embodiment of the present invention; Figure 2 These are three views of a first end plate and a second end plate provided according to an exemplary embodiment of the present invention; Figure 3 These are three views of an intermediate plate provided according to an exemplary embodiment of the present invention; Figure 4 These are three views of a sealing gasket provided according to an exemplary embodiment of the present invention; Figure 5 These are three views of a partition provided according to an exemplary embodiment of the present invention; Figure 6 This is a schematic flowchart of a method for preparing a chromatographic column apparatus according to an exemplary embodiment of the present invention.

[0026] In the picture: 100. Chromatographic column apparatus; 10. First end plate; 11. Inlet port; 20. Second end plate; 21. Outlet interface; 30. Chromatographic column layer; 31. Intermediate plate; 32. Sealing gasket; 33. Separator; 40. Vent holes; 50. Bolt holes; 60. Interface; 70. Ditch; 80. Sealing groove.

[0027] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the invention in any way, but rather to illustrate the concept of the invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0029] In the description of this invention, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0030] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0031] Existing chromatographic column devices suffer from numerous drawbacks, including poor performance consistency, low standardization, insufficient spatial integration, complex processes, high costs, limited adaptability, and poor reusability. To address these issues, this invention provides a chromatographic column device comprising a first end plate, a second end plate, and a column layer sandwiched between the first and second end plates. The column layer includes an intermediate plate, a sealing gasket, and a partition. At least one surface of the intermediate plate is forged with a groove. The sealing gasket adheres to and covers the grooved side of the intermediate plate, and the partition covers the outer side of the sealing gasket. The first end plate, the second end plate, the intermediate plate, the sealing gasket, and the partition are tightly connected to compress the sealing gasket, causing it to deform and allowing the sealing gasket and the groove of the intermediate plate to together enclose a chromatographic column channel. The chromatographic column channel is filled with chromatographic packing material.

[0032] This solution employs a forging process to integrally form the plate channel structure. By fastening and assembling the end plates, middle plate, sealing gasket, and partition, the sealing gasket and middle plate channel are pressed and fixed together. The compression sealing allows the sealing gasket to cooperate with the middle plate channel to form a complete chromatographic column fluid channel. This structural design fundamentally solves the industry difficulties of large-size chromatographic column preparation, such as high difficulty and uncontrollable molding quality. It significantly reduces the overall manufacturing process threshold and processing difficulty of chromatographic columns, effectively reduces the preparation and production cost, breaks through the industry technical barriers of traditional chromatographic column preparation, and enables the simplification, standardization, and large-scale application of chromatographic column preparation.

[0033] The following is a reference to the appendix. Figures 1 to 6 The preferred technical solution of the chromatographic column device 100 and its preparation method provided by the present invention will be described in detail.

[0034] like Figure 1 As shown, the chromatographic column device 100 disclosed in this invention adopts a modular stacked structure with upper and lower end plates sandwiching a multi-layered plate in the middle. The core includes a first end plate 10, a second end plate 20, and a chromatographic column layer 30 sandwiched between the two end plates. The overall structure is symmetrical and regular, easy to assemble and disassemble, and has strong molding stability. Among them, the chromatographic column layer 30 is the core functional layer, which is specifically composed of three functional plates: an intermediate plate 31, a sealing gasket 32, and a partition plate 33. Each plate is independently molded and assembled layer by layer, which is completely different from the traditional integrated long tube closed structure.

[0035] In terms of structural forming process, the core innovation of this invention lies in using a forging process to replace traditional etching, machining, casting, and bending forming processes in the preparation of the channel structure. Specifically, in the implementation process, as follows... Figure 3As shown, the single or double sides of the intermediate plate 31 are pressed into channels 70 of preset specifications using a precision forging and integral molding process. The forging and molding process can precisely control the width, depth, curvature, length and arrangement of the channels 70 according to the actual chromatographic separation requirements. The inner wall of the channel 70 after molding is smooth, with high dimensional accuracy and uniform deformation, without the burrs, grooves and dimensional deviations caused by traditional machining.

[0036] Furthermore, the channel 70 structure of this invention possesses extremely high design flexibility. Optionally, the layout of the channel 70 can adopt various regular structures such as a serpentine or U-shaped configuration. Simultaneously, the number of channels 70 can be flexibly increased or decreased according to equipment design parameters, detection throughput, and separation accuracy requirements, enabling the design of various functional structural combinations as needed. By combining different numbers and layouts of channels 70, multi-channel chromatographic arrangements in series or parallel can be achieved. Simultaneously, differentiated functional designs such as variable column diameter and variable packing material functions can be flexibly implemented, adapting to diverse application scenarios with varying sample separation difficulties, different media flow rates, and different detection accuracies.

[0037] Preferably, such as Figure 5 As shown, air passages 40 with different diameters and positions can be opened on the partition 33 according to the channel layout requirements. The differentiated air passages 40 can be used to accurately connect different independent channels 70 in the upper and lower layers, thereby realizing the controllable series combination between multiple channels 70. Multi-level series separation pathways can be flexibly built according to the sample separation gradient requirements, which greatly improves the layer separation effect of complex component samples.

[0038] Compared to the shortcomings of traditional long tube internal channels, such as simple structure, fixed layout, inability to expand functions, and uncontrollable size, the forging process can achieve standardized, batch, and customized molding of the channel 70. The channel 70 parameters of all intermediate plates 31 in a single batch are completely consistent, ensuring the regularity, consistency, and functional expandability of the chromatographic column channels from the structural source.

[0039] In terms of assembly structure, the sealing gasket 32 ​​completely fits and covers the opening side of the groove 70 of the intermediate plate 31, achieving full coverage and sealing of the groove 70 opening. The partition plate 33 is stacked on the outside of the sealing gasket 32, forming a three-layer composite chromatographic column layer 30 structure of "intermediate plate 31-sealing gasket 32-partition plate 33". After the overall assembly is completed, the axial fastening pressure of the first end plate 10 and the second end plate 20 is used to press and fix all the plates together. At the same time, the extrusion pressure between the plates causes the sealing gasket 32 ​​to undergo controllable elastic deformation. After deformation, the sealing gasket 32 ​​completely fits the edge contour of the groove 70, sealing all gaps, and together with the forged groove 70 of the intermediate plate 31, it forms a closed, regular, and leak-free chromatographic column channel. The chromatographic packing material is uniformly filled into the interior of the formed chromatographic column channel. The packing process is completed in the open plate structure, and the entire process is visible and controllable.

[0040] The core beneficial effects of this implementation method are reflected in the structural forming and assembly logic level: Firstly, the forging and pressing integrated molding process for the channel has high molding precision, good consistency, and simple process, which completely solves the problems of uncontrollable molding and disordered deformation of the internal channels of traditional long tube chromatography columns, and realizes the standardized molding of the channel structure.

[0041] Secondly, the open-plate, layer-by-layer assembly structure abandons the traditional closed tube structure, making the entire packing process visible. This allows for precise avoidance of defects such as voids, interlayers, and packing accumulation, ensuring uniform packing density and consistent structure within each column and significantly improving product performance consistency.

[0042] Thirdly, the modular stacking structure simplifies assembly, eliminating complex bending and post-forming processes, and completely preventing irreversible defects such as packing material breakage and pore distortion caused by traditional coiling processes. This improves the separation performance and lifespan of the chromatographic column from the source of the process. It should be noted that this invention can achieve the molding of large-size chromatographic column structures 100. The upper and lower limits of the molding size of the chromatographic column device 100 depend on the processing precision of the forging process. With the iterative improvement of the forging process precision, this invention can stably achieve the processing and preparation of large-size chromatographic column devices with millimeter-level and larger specifications, completely breaking through the technical bottleneck of traditional microchannel chromatographic columns being limited in size and unable to handle large-volume packing materials, thus adapting to general large-size chromatographic detection scenarios.

[0043] Fourth, this device adopts a modular and detachable assembly structure. The entire chromatographic column device 100 can be directly reassembled and used after the sealing gasket 32 ​​is disassembled and replaced. There is no need to replace the main plate structure. The sealing performance and detection accuracy of the equipment can be restored by simply replacing the consumable sealing accessories. This greatly reduces the cost of equipment use and consumable consumption. The reusability and economy of the equipment are significantly improved, which is different from the disadvantages of traditional chromatographic columns that are scrapped as a whole and cannot be partially replaced or repaired.

[0044] In some embodiments, the present invention adopts a universal material selection design for each plate component to meet different detection media, operating conditions, temperature and pressure environments, and cost requirements. The first end plate 10, the second end plate 20, the intermediate plate 31, the sealing gasket 32, and the partition plate 33 can all be independently selected from any one of metal materials, organic materials, and modified materials, and can be freely combined, with extremely strong adaptability.

[0045] In specific material application scenarios, metallic materials can include conventional industrial metals such as stainless steel, iron, copper, aluminum, and alloys. Stainless steel, with its resistance to acid and alkali corrosion, high temperature resistance, high mechanical strength, and resistance to deformation, is suitable for high-precision, high-pressure, and corrosive media chromatographic detection scenarios. Copper and aluminum have excellent thermal conductivity, making them suitable for detection scenarios requiring precise temperature control, and can be used with small temperature control systems to achieve rapid and uniform temperature control. Ordinary iron is inexpensive and suitable for general detection scenarios under normal pressure and without corrosive media, significantly reducing equipment costs. Organic materials can include high-molecular organic materials such as PP, PE, PVC, and polytetrafluoroethylene, which have advantages such as light weight, corrosion resistance, good insulation, and easy processing, making them suitable for lightweight portable chromatography equipment and low-temperature, normal-pressure detection scenarios. Modified materials can include glass fiber modified plastics, metal modified composite materials, and high-temperature resistant modified polymers, combining the advantages of both metallic and organic materials. They offer high strength, high temperature resistance, and aging resistance, making them suitable for special detection scenarios under extreme conditions.

[0046] To ensure the core effect of compression sealing, this invention strictly limits the matching relationship of material hardness: the material hardness of the sealing gasket 32 ​​must be significantly lower than that of the first end plate 10, the second end plate 20, the intermediate plate 31, and the partition plate 33, resulting in better deformability after compression molding. In specific implementation, the end plates, intermediate plates 31, and partition plates 33, as structural load-bearing and molding bases, are made of high-hardness, high-rigidity materials to ensure the overall structural dimensional stability and compressive strength, avoiding plate deformation and channel 70 distortion after tightening and pressure. The sealing gasket 32 ​​is made of low-hardness, high-elasticity, and high-deformation-recovery material, which can undergo uniform deformation under external tightening pressure, precisely conforming to the contour of the channel 70 and the gaps in the plate, filling all tiny gaps, achieving complete sealing, and preventing media leakage.

[0047] The advantages of this material selection scheme are mainly reflected in its versatility and economy: First, the multi-category material adaptability design breaks through the shortcomings of traditional chromatography columns with single materials and limited operating conditions. It can be flexibly selected according to equipment specifications, detection environment and cost budget, and has strong scalability. Second, the hardness matching design of the rigid matrix and flexible seal achieves the synergistic effect of rigid structure shaping and flexible structure sealing, which not only ensures the accuracy of the column channel size, but also guarantees the reliability of the seal, and completely solves the industry problem of plate column seal failure, liquid leakage and gas leakage. Third, conventional industrial materials can replace traditional special precision materials, which are easy to obtain and have low processing costs. They do not require customized special components, thus significantly reducing the production threshold and mass production costs.

[0048] To achieve precise alignment, uniform clamping, and reliable fixing of each modular plate, this invention provides bolt holes 50 of perfectly matched specifications and positions at corresponding positions on the edges of the first end plate 10, second end plate 20, intermediate plate 31, sealing gasket 32, and partition plate 33. All bolt holes 50 are coaxially aligned and have a uniform diameter, ensuring assembly alignment accuracy. Figures 1 to 5 .

[0049] During the assembly process, after each plate is stacked and aligned, high-strength bolts are inserted through the corresponding bolt holes 50 on all plates, and symmetrical pressure is applied and tightened using nuts at both ends of the bolts. The tightening process employs a double-sided synchronous pressure application technique, with the nuts tightened evenly at both ends to ensure that the axial pressure is evenly applied across the entire plate surface. This guarantees consistent pressure in every area and avoids problems such as plate misalignment, uneven local pressure, and deformation imbalance of the sealing gasket 32 ​​caused by unilateral pressure application. Compared to traditional snap-fit, unilateral pressure, and adhesive fixing methods, this bolt-through fastening structure offers stronger stability, higher pressure controllability, and better reproducibility in assembly and disassembly.

[0050] In implementing specific process parameters, the bolt tightening torque can be adjusted according to the column size, the material of the sealing gasket 32, and the operating pressure level. For low-pressure, ambient-temperature scenarios, a standard tightening torque is used, while for high-pressure testing scenarios, the tightening pressure can be increased to ensure the sealing gasket 32 ​​fully deforms and seals, adapting to different pressure conditions. Simultaneously, the bolt holes 50 are evenly distributed around the plate, ensuring uniform pressure across the entire column layer 30 plane, eliminating localized gaps and sealing failures, and achieving a comprehensive, all-around sealed assembly.

[0051] The beneficial effects of this assembly structure are as follows: Firstly, the through bolt fastening structure is simple and the assembly is highly standardized. No special precision equipment is required, and the assembly can be completed manually. There are no special requirements for the technical level of the operators, which greatly reduces the production and assembly threshold. Secondly, the symmetrical pressure application method ensures uniform pressure distribution, uniform and stable deformation of the sealing gasket 32, and strong consistency in sealing effect, avoiding batch performance differences. Third, the bolt-detachable connection structure supports repeated disassembly, maintenance, packing replacement, and component replacement, making the equipment highly reusable and solving the problems of traditional chromatography columns being formed in one go, unable to be maintained, and having a high scrap rate. Fourth, standardized hole assembly enables automated assembly line adaptation, facilitating large-scale, standardized mass production and widespread adoption in the future.

[0052] To further improve sealing reliability and prevent slippage, deformation, overflow, or partial voiding of the sealing gasket 32 ​​under high-pressure conditions, this invention provides limiting sealing structures on the relatively mating plate surfaces. Specifically, the intermediate plate 31 and the partition plate 33 are respectively provided with sealing grooves 80 that are positioned opposite each other and have matching contours. The two sets of sealing grooves 80 together form a bidirectional limiting sealing cavity, solving the shortcomings of weak limiting ability and insufficient sealing stability of the limiting structure. Among them, the sealing grooves 80 of the intermediate plate 31 are arranged around the outer contour of the channel 70, and are arranged around the entire channel 70, which can form a full-area sealing and blocking structure for the channel 70, ensuring the complete sealing of the channel 70 inlet and outlet and surrounding gaps from the root.

[0053] During the specific assembly and sealing process, before each plate is tightened and pressurized, the sealing gasket 32 ​​is precisely aligned and arranged between the intermediate plate 31 and the partition plate 33, completely corresponding to the sealing groove 80 areas arranged on both sides. At the same time, the sealing gasket 32 ​​completely covers the groove 70 of the intermediate plate 31 and its outer surrounding sealing groove 80. When tightening pressure is applied to both ends of the screw, the sealing gasket 32 ​​undergoes uniform elastic deformation under bidirectional axial compression. Part of the structure of the sealing gasket 32 ​​is simultaneously compressed and embedded into the two opposing sealing grooves 80. Through the bidirectional clamping and limiting effect of the outer surrounding sealing groove 80 of the intermediate plate 31 and the sealing groove 80 of the partition plate 33, the sealing gasket 32 ​​is constrained both vertically and horizontally and limited in the entire circumferential area. This effectively restricts the lateral slippage, misalignment, and excessive deformation of the sealing gasket 32, allowing the sealing gasket 32 ​​to completely fill the small gaps between the sealing grooves 80 on both sides and the plates. At the same time, it achieves all-round sealing and blocking of the outer periphery of the groove 70, forming a double-layer clamping full-area sealing structure with stronger airtightness and higher stability. Under high-pressure medium flow conditions, the medium pressure can further compact the mating surface of the sealing gasket 32 ​​and the bidirectional sealing groove 80, forming an adaptive pressure-boosting sealing effect, and completely solving the industry defects of traditional plate-type sealing structures that are prone to loosening, leakage, and sealing failure under high pressure.

[0054] The beneficial effects of this bidirectional sealing groove 80 clamping structure are: Firstly, the bidirectional opposing structure of the intermediate plate 31 surrounding the channel 70 sealing groove 80 and the partition plate 33 sealing groove 80 forms a dual constraint effect of bidirectional clamping and circumferential limiting on the sealing gasket 32. Compared with the traditional single-sided limiting and non-circular sealing structure, the limiting sealing effect is stronger, which can completely eliminate the sealing failure problem caused by the slippage, misalignment and voiding of the sealing gasket 32 ​​under high pressure and high flow rate conditions, and greatly improve the sealing stability and pressure resistance of the equipment. Secondly, the sealing groove 80 of the intermediate plate 31 surrounds the outside of the channel 70, which can form a full-area seal around the entire chromatographic channel. After the sealing gasket 32 ​​is embedded in the sealing groove 80 on both sides, it greatly increases the sealing contact area and sealing tightness, completely eliminating micro-leakage, gas leakage and liquid leakage around the channel 70, ensuring the overall airtightness of the chromatographic column channel, and effectively improving the stability of the detection baseline and the repeatability of experimental data. Third, the bidirectional alignment sealing groove 80 can precisely constrain the deformation range of the sealing gasket 32, evenly distribute the tightening pressure, avoid excessive local compression of the sealing gasket 32, which can lead to aging and damage, effectively extend the service life of the sealing gasket 32, and reduce the cost of later maintenance and consumable replacement of the equipment. Fourth, the double-sided groove alignment and limiting structure can further improve the assembly alignment accuracy of the intermediate plate 31, sealing gasket 32, and partition 33, accurately ensure the regularity of channel 70 forming, avoid channel distortion and media flow disorder caused by assembly offset, and further improve the overall separation performance and long-term working stability of the chromatographic column.

[0055] Furthermore, since the sealing process only involves the deformation of the sealing gasket 32 ​​to produce a sealing effect, the chromatographic column device 100 assembled in this invention has a reusable function that is not found in similar products, allowing for convenient and quick disassembly. It can be reused repeatedly simply by replacing the sealing gasket 32, thus improving the flexibility and economy of use.

[0056] To improve the corrosion resistance, oxidation resistance, wear resistance and surface density of the sealing gasket 32, the present invention deposits a dense sealing protective layer on the entire surface of the sealing gasket 32. The sealing protective layer is preferably made of silver or nickel and is prepared using mature processes such as vacuum coating, electroplating, and chemical plating. The coating has uniform thickness, strong adhesion, and is free of pinholes and cracks.

[0057] In practical implementation, the silver sealing protective layer possesses excellent chemical stability, corrosion resistance, and thermal conductivity. It is not prone to chemical reactions with commonly used chromatographic detection media such as acids, alkalis, and organic solvents, effectively preventing the sealing gasket 32 ​​from being corroded and aged by the media. Simultaneously, its excellent thermal conductivity, combined with the temperature control system, ensures uniform temperature across the entire chromatographic column. The nickel sealing protective layer boasts high hardness, strong wear resistance, and good oxidation resistance, effectively resisting wear caused by repeated disassembly, assembly, and deformation, preventing scratches and defects on the surface of the sealing gasket 32, and ensuring sealing accuracy over long-term use. The metal plating completely covers the micropores on the surface of the sealing gasket 32 ​​substrate, improving surface density and eliminating media penetration and micro-leakage problems.

[0058] The beneficial effects of this coating structure are as follows: the metal sealing protective layer can isolate the detection medium from direct contact with the substrate of the sealing gasket 32, greatly improving the corrosion resistance, aging resistance and wear resistance of the sealing gasket 32, and extending the service life of the equipment; the dense coating fills the micro-defects of the substrate, improves the flatness and sealing performance of the sealing surface, and eliminates micro-leakage; the coating process is mature, low-cost, and can be mass-produced, which greatly improves the stability of the equipment and the range of applicable working conditions without increasing the production cost too much.

[0059] The present invention adopts a modular stacking design. Multiple chromatographic column layers 30 can be flexibly stacked between the first end plate 10 and the second end plate 20 according to the requirements of chromatographic separation efficiency, detection accuracy and media processing volume. It can realize any number of stacks of two, three or more layers, and has extremely strong structural scalability.

[0060] In practical implementation, the multilayer chromatographic column layers 30 are assembled using a co-directional stacking and sequential alignment method. Each column layer 30 independently possesses a complete channel structure 70, sealing structure, and media flow structure. The multilayer structure enables multi-stage chromatographic separation, increases the packing volume, and extends the separation path, significantly improving the separation accuracy and efficiency of complex mixed media. Compared to the shortcomings of traditional single-specification long-tube chromatographic columns, which cannot be flexibly expanded or have their separation paths adjusted, this invention allows for the free addition or reduction of layers according to detection needs, adapting to different scenarios such as trace detection, routine detection, and high-flow-rate detection. Simultaneously, the multilayer stacked structure maintains a regular plate-like structure, resulting in high space utilization and adaptability to miniaturized integrated equipment, solving the problems of the large size and inability to integrate traditional long-tube chromatographic columns.

[0061] The advantages of this multi-layer stacked structure are: the modular multi-layer stacked design allows for flexible structural expansion, adapting to different separation accuracies and throughput requirements, and significantly improving the equipment's versatility; the multi-layer plate stacked structure has a neat layout and small space occupation, and can be adapted to miniaturized temperature control systems, enabling overall equipment miniaturization and integration upgrades; the multi-layer independent channel structure can achieve multi-stage separation, greatly improving the separation effect of complex samples and optimizing chromatographic detection performance.

[0062] In the basic scenario of stacked multilayer chromatographic column layers 30, to further simplify the structure, reduce volume, lower component costs, and improve assembly efficiency, this invention optimizes the design of a shared partition 33 structure. When the sides of the opposing intermediate plates 31 of two adjacent chromatographic column layers 30 are formed with channels 70 through a forging process, the two opposing chromatographic column layers 30 can share a single partition 33, eliminating the need for two separate partitions 33.

[0063] In practical implementation, the lower channel 70 of the upper chromatographic column layer 30 is arranged opposite to the upper channel 70 of the lower chromatographic column layer 30, with only one partition 33 in between. The upper and lower sides of the partition 33, in conjunction with the corresponding sealing gaskets 32, form independent chromatographic channels with the channel 70. The partition 33 simultaneously achieves the functions of isolation and sealing of the upper and lower channels. This structure makes full use of the bidirectional forged channel 70 structure, maximizing the simplification of the overall structure of the multi-layer stack, reducing the number of plate components, and lowering the overall thickness and weight of the equipment.

[0064] The beneficial effects of this shared partition 33 structure are: significantly reducing the number of parts, simplifying the overall structure of the equipment, and reducing processing and assembly costs; reducing the overall volume and weight of the multilayer chromatography column, further improving the integration and miniaturization of the equipment; reducing the assembly gap of the multilayer plate stack, improving the overall structural compactness and sealing stability, while simplifying the assembly process, improving assembly efficiency, and making it more suitable for large-scale mass production.

[0065] To further expand the structural forms of passageways, improve space utilization, and enrich passageway layout methods, such as Figure 2 As shown, the present invention also employs a forging process to integrally form a channel 70 structure on the inner surface of the first end plate 10 and / or the second end plate 20 facing the chromatographic column layer 30, so that the end plate not only serves as a clamping and fixing base, but also functions as a channel forming body. It should be noted that when the channel 70 structure is formed on the inner surface of the first end plate 10 and / or the second end plate 20 facing the chromatographic column layer 30, a sealing groove 80 should also be integrally formed around the channel 70 to limit the positioning of the sealing gasket 32.

[0066] In a specific implementation, the channel 70 can be formed only on the inner side of the first end plate 10, or only on the inner side of the second end plate 20, or simultaneously on the inner sides of both end plates, allowing for flexible selection based on channel layout requirements. One side of the end plate channel 70 is also fitted with a sealing gasket 32, forming a sealed chromatographic channel on the end plate side through tight compression. This channel, in conjunction with the channel in the intermediate plate 31, forms a composite multi-segment channel structure. This design fully utilizes the effective planar space of the end plates, increasing channel length and packing volume without the need for an additional intermediate plate 31, further optimizing equipment space utilization.

[0067] The beneficial effects of this structure are: it realizes the functional integration of the end plate, taking into account both the functions of fixing and clamping and channel forming, maximizing the use of plate space and simplifying the equipment structure; the forged end plate channel 70 has high precision and good consistency, and can be perfectly matched with the intermediate plate channel 31, optimizing the medium flow path and improving the separation effect; the flexible end plate channel 70 design can be adapted to a variety of channel layout schemes, further improving the flexibility of equipment structure design and the adaptability to various scenarios.

[0068] To achieve stable input, output, and layer-by-layer flow of the medium, this invention employs a medium flow structure consisting of end plate interfaces 60 and multi-layer aligned vent holes 40. Specifically, one of the first end plate 10 and the second end plate 20 has a medium inflow interface 11, and the other has a medium outflow interface 21, forming a unidirectional and stable medium flow direction. The interface 60 specifications can be flexibly selected to adapt to different needs. Simultaneously, vent holes 40 with consistent positions and diameters are correspondingly formed on the intermediate plate 31, sealing gasket 32, and partition plate 33 of the column layer 30. All vent holes 40 are coaxially aligned and interconnected, forming a medium flow path that runs through the entire multi-layer column structure.

[0069] In practice, the medium enters the equipment through the inlet 11, flows through the interconnected vents 40 into the channels of each chromatographic column, and after chromatographic separation is completed by the uniform packing material within the channels 70, it is discharged from the outlet 21 through the end vents 40. The entire process is smooth, without dead zones or stagnation. The vents 40 are precisely matched to the channel structure, and their pore size is standardized according to the medium's flow rate, viscosity, and pressure parameters, effectively avoiding problems such as medium turbulence, excessive pressure loss, and sample residue. The multi-layered, aligned vent structure ensures uniform medium distribution and stable flow rate in each column layer 30, improving the consistency of multi-layer separation.

[0070] It should be noted that this chromatographic packing material can be replaced with embedded tubes, coating materials, or 80-mesh 5A molecular sieve chromatographic packing columns as needed.

[0071] The beneficial effects of this flow structure are: standardized alignment of the 40-hole structure ensures smooth media flow, stable pressure, and minimal sample residue, thereby improving the accuracy and repeatability of chromatographic detection; the partitioned inlet and outlet design of the upper and lower end plates ensures neat pipeline connections, convenient assembly and disassembly, and compatibility with various chromatographic equipment pipeline systems; the multi-layer interconnected pathway design ensures synchronous and stable operation of the multi-layer chromatographic column (30 layers), uniform flow distribution, avoids single-layer overload and uneven operation, and significantly improves the overall operational stability of the equipment.

[0072] like Figure 6 As shown, the chromatographic column preparation method of the present invention adopts a standardized, visualized, and modular process throughout, which completely subverts the traditional closed packing process that relies on manual experience. Specifically, it includes four core steps: plate forging and forming, layer-by-layer visualized assembly, precise packing, and overall tight sealing. The process is simple, highly replicable, and has extremely low requirements for equipment and personnel.

[0073] S110, plate forging standardization forming. For example... Figures 2 to 5As shown, based on equipment dimensions, channel specifications, and operating requirements, suitable metal, organic, or modified materials are selected as base materials, and precision forging processes are used to prepare the first end plate 10, the second end plate 20, the intermediate plate 31, the sealing gasket 32, and the partition plate 33, respectively. Among them, the intermediate plate 31 and the end plates are integrally formed with single-sided or double-sided channels 70 using forging dies according to design requirements. The die precision is controllable, and the dimensions, depth, and curvature of all channels 70 in a single batch are completely uniform, achieving standardized structural forming. The forging process has a fast forming speed, high yield, and no material waste, enabling mass production. Compared with machining and etching processes, production costs are significantly reduced, and forming efficiency is greatly improved.

[0074] S120 features a layer-by-layer visualized stacking assembly. Employing an open, layer-by-layer laying logic, the first end plate 10, intermediate plate 31, sealing gasket 32, partition plate 33, and second end plate 20 are laid sequentially from bottom to top. Multi-layer structures follow this stacking logic synchronously. The entire assembly process is completely open and fully visible, with no closed or hidden structures. Operators can observe the alignment accuracy and flatness of each layer in real time, allowing for timely correction of misalignments and eliminating the unknown defects of traditional closed-loop assembly. It should be noted that in this assembly step, the number of intermediate plates 31, sealing gaskets 32, and partition plates 33 can be flexibly increased or decreased according to actual testing throughput, separation accuracy, and multi-stage separation functional requirements. This high degree of assembly flexibility allows it to adapt to different operating conditions, such as single-layer foundation testing and multi-layer multi-stage separation.

[0075] S130, Precision Visualized Packing. After each layer of intermediate plates 31 and channel 70 is laid, and before the sealing gaskets 32 and partitions 33 are compressed, the chromatographic packing material is uniformly and layered into the forged channel 70. During the filling process, the packing density and uniformity can be observed in real time. Manual fine-tuning to fill gaps and level the packing layer allows for precise control of packing density, eliminating problems such as voids, interlayering, and accumulation. This ensures a highly uniform packing distribution in each channel, guaranteeing the consistency and stability of the chromatographic column's separation performance from the process source. It should be noted that the packing filling process in this step can be carried out simultaneously with the layer-by-layer stacking assembly process in the second step. That is, after completing the laying of a single set of intermediate plates 31, the packing operation for the corresponding channel 70 can be carried out immediately, followed by the laying of the sealing gaskets 32 and partitions 33. There is no need to complete the stacking of all plates before uniform filling, significantly simplifying the assembly process, improving preparation efficiency, and further ensuring the filling accuracy of a single layer of packing material, avoiding problems such as packing displacement and uneven compaction that occur after multiple layers are stacked.

[0076] S140, integrally fastened and sealed. After all plates are stacked and the packing is filled, screws are inserted through all the bolt holes 50 on the plates, and a double-sided synchronous pressure tightening method is used to uniformly pressurize the entire structure, causing the sealing gasket 32 ​​to undergo controllable elastic deformation, tightly fitting the contour of the channel 70 and the gaps between the plates, forming a sealed, regular, and leak-free standardized chromatographic column channel, thus completing the overall preparation of the chromatographic column device 100. If problems such as uneven packing or misalignment occur during the preparation process, it can be directly disassembled and reassembled, making troubleshooting convenient and the repair cost extremely low.

[0077] The core benefits of this preparation method lie in its technological innovation: First, the forging process is simple and highly standardized, completely solving the problems of uncontrollable column forming and large batch-to-batch variations in traditional chromatographic columns, thus achieving standardized mass production. Second, the layer-by-layer visualized assembly and packing completely eliminate the random defects of traditional processes, significantly improving product consistency. Third, the process does not require high levels of operator skill or production equipment configuration, making it suitable for various research institutions and SMEs to produce independently, breaking down industry technical barriers. Fourth, the detachable assembly structure facilitates troubleshooting, maintenance, and repair, resulting in high equipment reuse rates and significantly reducing usage and production costs. Fifth, the process is adaptable to multiple materials, sizes, and multi-layer structures, offering strong design flexibility and scenario adaptability, simultaneously meeting both customized research needs and standardized mass production and sales requirements.

[0078] Based on the specific structure and process implementation methods described above, this invention has comprehensive and systematic technical advantages over existing technologies, and completely solves various industry pain points of existing chromatographic column technologies.

[0079] At the structural forming level, this invention employs a forging-in-one channel forming process, replacing traditional bending, etching, and machining processes. This results in high channel dimensional accuracy, a regular structure, and strong batch consistency, completely solving the problems of channel deformation, uncontrollable dimensions, and numerous internal defects in traditional long-tube chromatographic columns. It achieves standardized forming of the chromatographic column channel structure, fundamentally improving the stability and repeatability of chromatographic detection. The modular plate structure completely eliminates the spatial limitations of traditional long-tube structures, offering a regular layout and compact size. It can be adapted to miniaturized temperature control systems, promoting the integration and lightweight upgrading of chromatographic equipment.

[0080] In terms of material selection, this invention is compatible with a variety of materials, including metals, organic materials, and modified materials, allowing for flexible selection based on operating conditions, cost, and application scenarios, resulting in strong versatility and scalability. Furthermore, the combination of a rigid matrix and flexible seal hardness matching design, a metal plating reinforcement design, and an 80° sealing groove limit design significantly improves sealing reliability, corrosion resistance, and wear resistance, overcoming the shortcomings of traditional plate chromatography columns such as poor sealing, short lifespan, and limited operating conditions.

[0081] At the assembly process level, this invention adopts a layer-by-layer visual stacking assembly, with full control over the filling process, completely eliminating defects of traditional processes such as voids, interlayers, filler breakage, and uneven stacking, significantly improving product performance consistency. The bolt-through fastening structure is simple to assemble, can be repeatedly disassembled and reassembled, and is easy to maintain, requiring very little from personnel and equipment. It breaks through the manual experience barrier of traditional technology, realizing the popularization and standardization of the process.

[0082] At the level of industrialization and practical application, this invention features a simple process, readily available materials, and low cost, enabling large-scale mass production. It also supports customized sizes and universal specifications, catering to both research customization and market penetration needs. The extended structure, including multi-layer stacking, shared partitions, and end-plate molding, further enriches the product system, adapting to all scenarios of testing needs, including micro-volume, routine, high-flow, and high-precision testing. It completely solves the industry problems of traditional technologies, such as difficulty in popularization, high cost, poor adaptability, and poor repeatability, possessing extremely high research and industrialization promotion value.

[0083] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A chromatographic column apparatus, characterized in that, It includes a first end plate, a second end plate, and a chromatographic column layer sandwiched between the first end plate and the second end plate; The chromatographic column includes an intermediate plate, a sealing gasket, and a partition. At least one side of the intermediate plate is forged and formed with a groove. The sealing gasket is fitted and covers the groove opening side of the intermediate plate, and the partition covers the outside of the sealing gasket. The first end plate, the second end plate, the intermediate plate, the sealing gasket, and the partition are fastened together to compress the sealing gasket and deform it, so that the sealing gasket and the groove of the intermediate plate together enclose the chromatographic column channel, and the chromatographic column channel is filled with chromatographic packing material.

2. The chromatographic column apparatus according to claim 1, characterized in that, The materials used to prepare the first end plate, the second end plate, the intermediate plate, the sealing gasket, and the partition are selected from any one of metallic materials, organic materials, and modified materials; The material hardness of the sealing gasket is less than that of the materials of the first end plate, the second end plate, the intermediate plate, and the partition.

3. The chromatographic column apparatus according to claim 2, characterized in that, Bolt holes are provided on the first end plate, the second end plate, the intermediate plate, the sealing gasket, and the partition plate respectively; By passing a screw through the corresponding bolt holes of each component and applying pressure to both ends of the screw, the first end plate, the second end plate, the intermediate plate, the sealing gasket, and the partition plate are pressed, fixed, and sealed.

4. The chromatographic column apparatus according to claim 2, characterized in that, The intermediate plate and the partition plate are respectively provided with sealing grooves. On the intermediate plate, the sealing groove surrounds the outside of the channel. When the connection is fastened, part of the structure of the sealing gasket is squeezed into the two opposing sealing grooves.

5. The chromatographic column apparatus according to claim 2, characterized in that, The surface of the sealing gasket is coated with a sealing protective layer, which is made of silver or nickel.

6. The chromatographic column apparatus according to any one of claims 1 to 5, characterized in that, Multiple layers of the chromatographic column are stacked between the first end plate and the second end plate.

7. The chromatographic column apparatus according to claim 6, characterized in that, When the opposite sides of the intermediate plates of two adjacent chromatographic column layers are both formed with channels by forging, the opposite channel sides of the two chromatographic column layers share a partition plate.

8. The chromatographic column apparatus according to any one of claims 1 to 7, characterized in that, The inner surface of the first end plate and / or the second end plate facing the chromatographic column layer is formed with grooves by a forging process; In this embodiment, the groove opening side of the first end plate and / or the second end plate is fitted and covered with a sealing gasket.

9. The chromatographic column apparatus according to any one of claims 1 to 7, characterized in that, One of the first end plate and the second end plate is provided with a medium inflow interface, and the other is provided with a medium outflow interface; The intermediate plate, sealing gasket and partition of the chromatographic column are all provided with corresponding air passages, and the air passages are interconnected to form a medium flow path. Preferably, different air vents can be provided on the partition to achieve series connection between different channels.

10. A method for preparing a chromatographic column apparatus, characterized in that, Suitable for a chromatographic column apparatus according to any one of claims 1 to 9, comprising: According to the usage requirements, a first end plate, a second end plate, an intermediate plate, a sealing gasket, and a partition are prepared by forging process, wherein at least one side surface of the intermediate plate is forged and formed with a groove. The first end plate, the middle plate, the sealing gasket, the partition and the second end plate are assembled in sequence by stacking and laying layer by layer. During the assembly process, the chromatographic packing is evenly filled into the groove of the middle plate, and the sealing gasket and the partition are sequentially attached and laid on the groove opening side of the middle plate. The assembled components are tightened and connected, causing the sealing gasket to deform under pressure and cooperate with the groove of the intermediate plate to form a sealed chromatographic column channel, thus completing the preparation of the chromatographic column device.

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