A method for promoting germination of cistanche seed

Abstract

The application discloses a method for promoting seed germination of Cistanche, and the seed germination of Cistanche is promoted by physical low-temperature induction and chemical signal cooperation. A parameter system of definite parameters of low-temperature, time length, chemical signal concentration and action time is provided. On the basis of effectively improving the seed germination rate of Cistanche, the standardization of seed germination conditions is realized, and the method is easy to popularize and mass-produce.

Description

Technical Field

[0001] This invention belongs to the field of breeding technology, specifically a method for promoting the germination of Cistanche deserticola seeds. Background Technology

[0002] Cistanche deserticola Ma is a rare and endangered plant used for both medicinal and edible purposes, possessing extremely high ecological, economic, and social value. Its unique resource characteristics and diverse values ​​make it a species with significant research and development potential. Seed germination and parasitism establishment are the core steps in the process of obligate root parasitic plants. Under natural conditions, seeds break dormancy and complete parasitism under the stimulation of secretions from the corresponding host root system. This process is regulated by multiple factors, including environmental temperature and humidity, soil microorganisms, and host condition. Natural germination rate and parasitism success rate are unstable and asynchronous, leading to unstable yields and a long time required for Cistanche deserticola production in artificial inoculation.

[0003] Currently, there are two main approaches to improving the germination rate of Cistanche deserticola seeds and promoting synchronous seed germination: chemical signal induction or physical and environmental regulation. Chemical signal induction pathways, which involve adding exogenous simulated or extracted germination stimuli to induce seed germination, primarily involve applying extracts from host plant roots or chemically synthesized signaling substances (such as strigolactones). While these methods can promote germination to some extent, they suffer from problems such as cumbersome preparation processes, unstable effects, and asynchronous germination. Furthermore, excessive use can negatively impact soil ecology and host plants, hindering sustainable development. Physical and environmental regulation pathways, which involve pretreating seeds with physical factors such as temperature, moisture, and light to alter their physiological state and increase their sensitivity to germination signals, have limited effectiveness in promoting seed germination. Both of these pathways are ineffective in inducing Cistanche deserticola seed germination, and existing chemical induction methods have certain limitations. Summary of the Invention

[0004] The purpose of this invention is to provide a method for promoting the germination of Cistanche deserticola seeds, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a method for promoting the germination of Cistanche deserticola seeds, comprising the following steps: S1: Physical low temperature induction treatment: Select seeds with a particle size greater than 0.6mm for cleaning, put the treated seeds into a breathable cloth bag and then carry out low temperature induction. The low temperature induction environment is: place the seeds in a constant temperature refrigerator at 0-5℃ for 30-60 days. S2: After the low temperature induction is completed, the seeds are taken out of the low temperature environment and placed at room temperature for one day to restore the seed metabolic activity. After one day, the seeds are soaked in 0.5% potassium permanganate for 0.5-1 hour to disinfect the seed surface. Then, the seeds are rinsed with distilled water and the moisture on the seed surface is removed. S3: Chemical signal triggering treatment: Place a filter paper in a sterilized petri dish, place the sterilized seeds on the filter paper, and add 4 mL of 10 mg / L fluazinam to every 50 seeds, depending on the number of seeds. After adding the fluazinam, place another layer of filter paper on top of the seeds and seal the dish, and make venting holes on the sealed surface. Finally, place the petri dish in a 25℃ constant temperature and light-proof incubator to cultivate the seeds to germinate.

[0006] Preferably, in step S1, the thousand-seed weight of the cleaned seeds is measured, and the seeds are preliminarily screened for quality, selecting seeds with a thousand-seed weight of 0.07 grams or more; and the low-temperature induction condition is to store the seeds at a constant temperature of 5°C for 60 days.

[0007] Preferably, in step S2, the potassium permanganate soaking time is 0.5 hours, and the seeds are soaked in a seedling bag with a thickness of 0.03 mm for every 100 seeds. This can solve the problem that the seeds of Cistanche deserticola float on the liquid and are not completely disinfected due to their small size and light weight. When rinsing with distilled water, the seeds are rinsed together with the seedling bag until the distilled water is colorless. Then the seeds are taken out of the seedling bag and the surface moisture is absorbed with filter paper. Rinsing the seeds together with the seedling bag can prevent the seeds from scattering and being lost during the rinsing process.

[0008] Compared with the prior art, the beneficial effects of the present invention are: This invention promotes the germination of Cistanche deserticola seeds through physical low-temperature induction and chemical signal synergy. It provides a parameter system with clear parameters for low temperature, duration, chemical signal concentration, and action time. While effectively improving the germination rate of Cistanche deserticola seeds, it also standardizes the seed germination conditions, making it easy to promote and scale up production. Attached Figure Description

[0009] Figure 1 This is a statistical bar chart of seed germination rate provided in an embodiment of the present invention. Detailed Implementation

[0010] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0011] This invention provides a technical solution: a method for promoting the germination of Cistanche deserticola seeds, comprising the following steps: S1: Physical low temperature induction treatment: Select seeds with a particle size greater than 0.6 mm for cleaning, conduct thousand-seed weight measurement on the cleaned seeds, conduct preliminary quality screening of the seeds, select seeds with a thousand-seed weight of 0.07 g or more, put them into a breathable cloth bag and then conduct low temperature induction. The low temperature induction environment is: place the seeds in a constant temperature refrigerator at 5℃ and store them for 60 days. S2: After the low temperature induction is completed, the seeds are taken out of the low temperature environment and placed at room temperature for one day to restore the seed metabolic activity. After one day, 100 seeds are placed in a seedling bag with a thickness of 0.03 mm. The seeds are soaked in 0.5% potassium permanganate for 0.5 hours to disinfect the seed surface. Then, the seeds and seedling bags are rinsed together until the distilled water used for rinsing is colorless. The seeds are then taken out of the seedling bags and the surface moisture is absorbed by filter paper. S3: Chemical signal triggering treatment: Place a filter paper in a sterilized petri dish, place the sterilized seeds on the filter paper, and add 4 mL of 10 mg / L fluazinam to every 50 seeds, depending on the number of seeds. After adding the fluazinam, place another layer of filter paper on top of the seeds and seal the dish, making ventilation holes on the sealed surface. Finally, place the petri dish in a 25℃ constant temperature and light-proof incubator to cultivate the seeds to germinate.

[0012] In this embodiment, three replicate experiments were conducted on seeds from different sources according to the method of the present invention. An experiment using water cultured at room temperature (25℃) served as a control group. During seed germination, the first observation was conducted on day 21, and the germination rate was recorded. The germination rate peaked on day 30, and the entire germination rate measurement was completed on day 37. The results are shown in Table 1 and... Figure 1 As shown in Table 1, when using the method of the present invention to promote the germination of Cistanche deserticola seeds, the seed germination period is concentrated between 20 and 30 days after sowing. The germination period of the method of the present invention is more concentrated than that of the control group, and the final seed germination rate is higher.

[0013] Table 1. Daily germination rate (%) of seeds at different observation times 5.21 Bed making 6.12 21 7 6 13 6.21 30 62 54 68 29 6.28 37 12 20 19 9 Total germination rate 81 80 87 51 .

[0014] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for promoting the germination of Cistanche deserticola seeds, characterized in that, Includes the following steps: S1: Physical low temperature induction treatment: Select seeds with a particle size greater than 0.6mm for cleaning, put the treated seeds into a breathable cloth bag and then carry out low temperature induction. The low temperature induction environment is: place the seeds in a constant temperature refrigerator at 0-5℃ for 30-60 days. S2: After the low temperature induction is completed, the seeds are taken out of the low temperature environment and placed at room temperature for one day to restore the seed metabolic activity. After one day, the seeds are soaked in 0.5% potassium permanganate for 0.5-1 hour to disinfect the seed surface. Then, the seeds are rinsed with distilled water and the moisture on the seed surface is removed. S3: Chemical signal triggering treatment: Place a filter paper in a sterilized petri dish, place the sterilized seeds on the filter paper, and add 4 mL of 10 mg / L fluazinam to every 50 seeds, depending on the number of seeds. After adding the fluazinam, place another layer of filter paper on top of the seeds and seal the dish, and make venting holes on the sealed surface. Finally, place the petri dish in a 25℃ constant temperature and light-proof incubator to cultivate the seeds to germinate.

2. The method for promoting the germination of Cistanche deserticola seeds according to claim 1, characterized in that, In step S1, the thousand-seed weight of the cleaned seeds is measured to conduct preliminary quality screening, and seeds with a thousand-seed weight of 0.07 grams or more are selected; and the low-temperature induction condition is to store the seeds at a constant temperature of 5°C for 60 days.

3. The method for promoting the germination of Cistanche deserticola seeds according to claim 1, characterized in that, In step S2, the seeds are soaked in potassium permanganate for 0.5 hours. During soaking, 100 seeds are placed in a seedling bag with a thickness of 0.03 mm and then soaked. When rinsing with distilled water, the seeds are rinsed together with the seedling bag until the distilled water is colorless. Then, the seeds are taken out of the seedling bag and the surface moisture is absorbed with filter paper.

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