A seedling raising method for improving sprouting of crude acacia

Abstract

This invention relates to the field of forest tree seed germination and seedling cultivation technology, and discloses a method for improving the germination of *Cephalotaxus fortunei* (a type of tree). The method includes: air-drying the fruit of *Cephalotaxus fortunei* and removing the outer seed coat to obtain *Cephalotaxus fortunei* seeds; storing the seeds in a low-temperature environment; soaking them in a potassium permanganate solution, followed by rinsing with running water and allowing them to drain; soaking the seeds in an exogenous gibberellin aqueous solution until they absorb water, swell, and reach a physiological germination state; wrapping the seeds in a sterile, purified water-moistened, defatted pure cotton towel and cultivating them in a light-protected environment with relatively constant temperature and humidity until the radicle tissue penetrates the outer seed coat and shows an elongated, white bud, then sowing and seedling cultivation. This invention improves the water absorption, disinfection, and germination conditions of *Cephalotaxus fortunei* seeds through air-drying and peeling, low-temperature storage, sterilization and drainage, gibberellin soaking, and moist wrapping cultivation, thereby enhancing the germination effect of *Cephalotaxus fortunei* seeds.

Description

Technical Field

[0001] This invention relates to the field of forest tree seed germination and seedling cultivation technology, specifically a method for improving the germination of *Pterocarya stenoptera*. Background Technology

[0002] Conventional seedling cultivation of *Symplocos macrantha* uses sand stratification. However, due to the dormancy characteristics of *Symplocos macrantha* seeds, the seedling cultivation cycle using sand stratification is relatively long, resulting in only one batch of seedlings being produced per year. This cannot meet the needs of large-scale production. With the upgrading of plant propagation systems, the industry has developed a tissue culture seedling system based on *Symplocos macrantha* stem segments. However, tissue culture seedlings not only have high input costs, but also require hardening-off before being released from the nursery, which prolongs the overall seedling emergence time. To address the issues of long cycles and high costs associated with existing seedling cultivation methods, introducing plant hormone treatment to break the dormancy state of *Symplocos buergeriana* seeds has become a viable technological direction. By relying on plant hormone treatment to promote seed germination, year-round seedling cultivation can be achieved. The overall seedling cost is lower than that of tissue culture seedlings, and the seedlings obtained through propagation are of superior quality compared to tissue culture seedlings. Currently, the industry urgently needs to develop a seedling cultivation method that integrates hormone treatment with corresponding physical environmental control to steadily improve the germination efficiency of *Symplocos buergeriana* seeds. Summary of the Invention

[0003] To address the shortcomings of existing technologies, this invention provides a seedling cultivation method that improves the germination of *Phyllostachys edulis*, solving the problems of long propagation cycles due to seed dormancy in traditional sand storage, high costs and slow emergence of tissue culture seedlings, and seed rot caused by oxygen deficiency during conventional germination processes.

[0004] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a method for improving the budding of *Phyllostachys edulis*, comprising the following steps: Harvest the fruits of the *Symplocos buergeriana*, air-dry the fruits and remove the outer seed coat to obtain the seeds of *Symplocos buergeriana*. The seeds of the coarse husk tree were stored in a low-temperature environment; After soaking the stored seeds in a potassium permanganate solution, rinse them with running water and let them stand in a ventilated environment to drain. Prepare an exogenous gibberellin aqueous solution, and soak the dried seeds of *Symplocos buergeriana* in the exogenous gibberellin aqueous solution to allow the seeds to absorb water, swell, and reach the physiological germination state. After soaking, remove and drain the seeds. Wrap the seeds of *Symplocos buergeriana* in a degreased pure cotton towel that has been sterilized at high temperature and moistened with sterile purified water. Place the seeds in a dark environment for cultivation, maintain the temperature and relative humidity of the cultivation environment, and spray sterile purified water onto the surface of the degreased pure cotton towel regularly. When the radicle tissue penetrates the outer seed coat of the coarse husk tree seed and shows an elongated, white appearance, remove the degreased pure cotton towel wrapping and proceed with sowing and seedling cultivation.

[0005] Preferably, the fruit of *Symplocos buergeriana* that has reached physiological maturity and whose outer seed coat exhibits a natural mature color is harvested. The fruits of the *Symplocos lucida* were laid flat in a single layer on a carrier with good air circulation and exposed to the air in a cool, well-ventilated environment without direct sunlight. The flowing air carried away the moisture that evaporated from the surface of the fruits.

[0006] Furthermore, when air-drying the fruit of the *Symplocos rubra* tree, the endpoint of the dehydration process is determined based on the surface morphological changes of the outer seed coat of the fruit. When the outer seed coat of the fruit of the *Symplocos buergeriana* shrinks in volume due to water loss, and fine wrinkles form on the surface of the outer seed coat, and the outer seed coat can be peeled off by manual rubbing to separate the *Symplocos buergeriana* seeds without any attached substances on the surface, mechanical or manual rubbing force is applied to the *Symplocos buergeriana* fruit that has reached the end of the dehydration process, causing the outer seed coat of the *Symplocos buergeriana* fruit to break and fall off the surface of the *Symplocos buergeriana* seeds.

[0007] Preferably, the seeds of *Symplocos lucida* obtained after removing the outer seed coat of the fruit are placed in a breathable packaging container made of nylon mesh bags, wherein the pore size of the nylon mesh bags is smaller than the diameter of the *Symplocos lucida* seeds. A breathable packaging container is placed inside the storage module. A thermocouple obtains the temperature of the storage module's interior space, and a refrigeration unit uses a compressor to cool the seeds, thus performing low-temperature storage treatment.

[0008] Furthermore, the seeds of the coarse husk tree, which have been rinsed with running water, are transferred to a ventilated environment and left to stand and dry. When the surface of the coarse husk seed loses its reflective luster and no water film adheres between adjacent coarse husk seeds, the static filtration is completed, ensuring that no free water adheres to the outer seed coat surface of the coarse husk seed.

[0009] Preferably, when preparing an exogenous gibberellin aqueous solution, commercially available gibberellin powder is obtained, and the mass of commercially available gibberellin powder is calculated based on the absolute effective mass concentration of the exogenous gibberellin aqueous solution to be prepared, the purity percentage of the commercially available gibberellin powder, and the solvent volume. The commercially available gibberellin powder was weighed and dissolved in a portion of the solvent, and then the solvent was used to prepare an aqueous solution of exogenous gibberellin, which was gibberellin GA3.

[0010] Furthermore, the dried seeds of *Symplocos buergeriana* are transferred to the cultivation soaking tank of the soaking module, and an exogenous gibberellin aqueous solution is injected into the cultivation soaking tank to completely submerge the seeds.

[0011] Preferably, during the cultivation of *Symplocos buergeriana* seeds, the moisture content of a degreased pure cotton towel that has been sterilized at high temperature and moistened with sterile purified water is checked, and sterile purified water is sprayed onto the surface of the degreased pure cotton towel that has been sterilized at high temperature and moistened with sterile purified water.

[0012] Furthermore, sterile purified water is sprayed onto the surface of the degreased pure cotton towel that has undergone high-temperature sterilization and is moistened with sterile purified water, so that the degreased pure cotton towel that has undergone high-temperature sterilization and is moistened with sterile purified water remains moist and that no free liquid drips from the degreased pure cotton towel that has undergone high-temperature sterilization and is moistened with sterile purified water when squeezed.

[0013] Preferably, during the cultivation of *Symplocos lucida* seeds, the germination status of *Symplocos lucida* seeds in a physiological germination state is checked; When moldy or rotten seeds of the coarse husk tree are found, they should be picked out and removed.

[0014] This invention provides a method for improving the budding of *Phyllostachys edulis* seedlings. It has the following beneficial effects: 1. This invention reduces the impact of the outer seed coat of the fruit on water absorption and gas exchange of the seeds by air-drying the fruit and removing it by mechanical or manual rubbing. At the same time, the outer seed coat of the seeds is retained, providing conditions for subsequent storage, disinfection and soaking treatment.

[0015] 2. This invention reduces fungi, bacteria, and potassium permanganate solution residues on the outer seed coat surface of *Symplocos buergeriana* seeds through continuous treatment including low-temperature storage, soaking in potassium permanganate solution, rinsing with running water, and static filtration. It also reduces the secondary dilution of free water by exogenous gibberellin aqueous solution, thus maintaining stable soaking conditions in subsequent processes.

[0016] 3. This invention uses exogenous gibberellin aqueous solution to bring the seeds of *Symplocos buergeriana* to a physiological germination state, and then uses a degreased pure cotton towel that has been sterilized at high temperature and moistened with sterile purified water for cultivation. With the help of temperature, relative humidity, light protection and water replenishment, the humid environment required for the germination of *Symplocos buergeriana* seeds is maintained, thereby improving the germination effect of *Symplocos buergeriana* seeds. Attached Figure Description

[0017] Figure 1 This is the timing interaction diagram of the present invention; Figure 2 This is a schematic diagram of the method steps of the present invention; Figure 3 This is a schematic diagram illustrating the effect of changes in storage morphology according to the present invention; Figure 4 This is a topology diagram of the *Pterocarya stenoptera* seed treatment system of the present invention; Figure 5 This is a schematic diagram illustrating the germination of gibberellin seeds stored at 5°C according to the present invention. Figure 6 This is a schematic diagram illustrating the percentage of germination rate of seeds treated with gibberellin at 5°C according to the present invention. Figure 7 This is a schematic diagram illustrating the germination of seeds treated with gibberellin at room temperature according to the present invention. Figure 8 This is a schematic diagram illustrating the percentage of germination rate of seeds treated with gibberellin at room temperature according to the present invention. Detailed Implementation

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] See attached document Figure 1 This invention provides a seedling system for improving the germination of *Phyllostachys edulis*, the system comprising: The pretreatment module is used to air-dry the fruit of *Symplocos buergeriana* and remove the outer seed coat of the fruit to separate the seeds of *Symplocos buergeriana*. The storage module is used to store the seeds of *Symplocos buergeriana* obtained after removing the outer seed coat of the fruit in a low-temperature environment. The disinfection module is used to soak the seeds of the *Symplocos lucida* in chemical agents and to rinse and drain the seeds with running water. The soaking module is used to contain the prepared exogenous gibberellin aqueous solution and to soak the seeds of *Symplocos buergeriana* using the exogenous gibberellin aqueous solution. The germination module is used to cultivate treated coarse husk seeds in a dark environment with a temperature maintained between 26°C and 30°C and a relative humidity of 85%.

[0020] The fruits of the *Symplocos buergeriana* are processed by a pretreatment module, and the separated *Symplocos buergeriana* seeds are then processed sequentially by a storage module, a disinfection module, a soaking module, and a germination module.

[0021] The pretreatment module includes a carrier with air circulation, where the outer seed coat of the fruit of the *Symplocos buergeriana* is removed by mechanical or manual rubbing. The storage module includes the internal space of the storage module, refrigeration components, a compressor, and thermocouples. The disinfection module includes a soaking container, a rinsing filter, and a water supply line. The soaking module includes a cultivation soaking tank. The germination module includes a cultivation tray, which is placed in a light-protected environment with a temperature maintained between 26°C and 30°C and a relative humidity of 85%.

[0022] See attached document Figure 2 This invention provides a method for improving the germination of *Phyllostachys edulis* seedlings, the method comprising the following steps: S1, the harvested fruits of the *Symplocos lucida* are air-dried, the outer seed coat of the fruits is removed, and the seeds of the *Symplocos lucida* are separated. S2, store the seeds of the coarse chrysanthemum in a low-temperature environment of 5℃ for 2 months; S3, soak the seeds of *Symplocos buergeriana* that have been stored at low temperature in a 1% potassium permanganate solution for 30 minutes, then rinse with running water for 10 minutes and drain. S4, soak the seeds of *Symplocos buergeriana* in an aqueous solution of exogenous gibberellin with an absolute effective mass concentration of 400 mg / L for 24 hours; S5. Place the treated *Symplocos buergeriana* seeds in a dark environment with a temperature maintained between 26℃ and 30℃ and a relative humidity of 85% for germination. After 15 days of cultivation, check the germination status of the *Symplocos buergeriana* seeds and remove any moldy or rotten seeds. After 30 days of cultivation, stop the germination process and sow the seeds with radicle tissue penetrating the outer seed coat and showing an elongated, white-tipped appearance for seedling cultivation.

[0023] See attached document Figure 3 The S1 provided by the present invention includes the following steps in a specific implementation: S11. Harvest *Cephalotaxus fortunei* fruits that have reached physiological maturity and whose outer seed coat exhibits a naturally mature color. The outer seed coat of *Cephalotaxus fortunei* fruits is a layer with a high water content that wraps around the fruit. The outer seed coat of *Cephalotaxus fortunei* fruits is a different structure from the outer seed coat of *Cephalotaxus fortunei* seeds. The peeling operation only removes the outer seed coat of *Cephalotaxus fortunei* fruits, not the outer seed coat of *Cephalotaxus fortunei* seeds.

[0024] When freshly harvested, the outer seed coat of the *Cephalotaxus fortunei* fruit tightly adheres to the inner seed, hindering water absorption and gas exchange. The *Cephalotaxus fortunei* fruits are laid flat in a single layer on a well-ventilated surface and exposed to air in a cool, well-ventilated area at a temperature below 30°C. The exposure environment is kept well-ventilated and free from direct sunlight, allowing the airflow to remove moisture evaporated from the fruit surface.

[0025] S12, maintain the fruit of the *Symplocos lucida* in the exposed treatment environment for 2 to 3 days, and determine the endpoint of the dehydration operation based on the surface morphological changes of the outer seed coat of the *Symplocos lucida* fruit. As water is lost, the outer seed coat of the fruit of the *Symplocos buergeriana* shrinks in volume. As the volume shrinks, fine wrinkles form on the surface of the outer seed coat. When the area of ​​fine wrinkles on the surface of the fruit reaches more than half of the total surface area of ​​the fruit, and the outer seed coat can be peeled off by manual rubbing and the seeds of *Symplocos buergeriana* with no attached substances can be separated, the fruit reaches the micro-wrinkled state. The micro-wrinkled state is taken as the endpoint of the dehydration process.

[0026] S13, apply mechanical or manual rubbing force to the fruit of the coarse husk tree that has reached the slightly wrinkled state, so that the outer seed coat of the coarse husk tree fruit breaks and falls off from the surface of the coarse husk tree seed, and separate pure coarse husk tree seeds with no adhering substances on the surface. After the outer seed coat of the fruit of the *Symplocos buergeriana* is dehydrated, the adhesion between the outer seed coat and the inner seed is reduced. After the outer seed coat is removed, the seed, which has no attached material, comes into direct contact with the external environment and can absorb external moisture and oxygen.

[0027] The S2 provided by this invention includes the following steps in its specific implementation: S21, put the coarse husk tree seeds with no attached material on the surface into a breathable packaging container made of nylon mesh bag. The pore size of the nylon mesh bag is smaller than the diameter of the coarse husk tree seeds with no attached material on the surface. Place the breathable packaging container into the internal space of the storage module with the operating temperature set at 5°C. The breathable packaging container maintains gas exchange between the inside and outside of the storage microenvironment. The storage module has a built-in refrigeration component. Thermocouples obtain the temperature of the internal space of the storage module, and the refrigeration component cools the space through a compressor to keep the internal space of the storage module at 5°C.

[0028] S22, the seeds of the coarse husk tree with no attached substances on the surface are placed in the internal space of the 5℃ storage module for 2 months to complete the low temperature storage treatment.

[0029] S23. After the low-temperature storage treatment is completed, the coarse husk tree seeds with no attached substances on the surface are transferred from the internal space of the storage module to the disinfection module.

[0030] See attached document Figure 4 The S3 provided by the present invention includes the following steps in a specific implementation: S31, the coarse husk seeds with no surface attachments that have undergone low-temperature storage treatment at 5℃ are transferred to the soaking container of the disinfection module. A 1% potassium permanganate solution is injected into the soaking container. The 1% potassium permanganate solution is prepared according to the principle of 10g potassium permanganate per 1L of potassium permanganate solution until the potassium permanganate solution completely submerges the coarse husk seeds with no surface attachments. Seeds of *Symplocos lucida* with no attached organisms were soaked in a 1% potassium permanganate solution for 30 minutes to treat fungi and bacteria attached to the outer seed coat.

[0031] S32. After soaking in potassium permanganate solution, transfer the seedlings of *Symplocos buergeriana* with no adhering substances to the soaking container and place them into a rinsing filter with drainage holes at the bottom. Use the water supply line connected to the disinfection module to supply flowing clean water to the seedlings in the rinsing filter and continue rinsing for 10 minutes. The flowing clean water removes the potassium permanganate solution residue on the surface of the seedlings, reducing the impact of potassium permanganate solution residue on subsequent exogenous gibberellin aqueous solution treatment.

[0032] S33, shut off the water supply line connected to the disinfection module and stop the running water rinsing operation. Transfer the coarse sand tree seeds that have been rinsed with running water and have no adhering substances to the filter screen to a ventilated environment and let them stand to dry. When the surface of *Gnaphalium affine* seeds without any adhering substances loses its reflective luster and no water film adheres between adjacent seeds without adhering substances, the static filtration and drying process is complete. After static filtration and drying, the outer seed coat of the *Gnaphalium affine* seeds will not have any free water adhering to it, thus avoiding secondary dilution of the subsequent exogenous gibberellin aqueous solution by free water. The chemical waste liquid generated by the disinfection module is collected and treated in accordance with existing biochemical laboratory waste liquid treatment methods.

[0033] The S4 provided by this invention includes the following steps in a specific implementation: S41. Obtain commercially available gibberellin powder. Based on the absolute effective mass concentration of the exogenous gibberellin aqueous solution to be prepared, the purity percentage of the commercially available gibberellin powder, and the solvent volume, calculate the mass of the commercially available gibberellin powder to be weighed. The formula for calculating the mass of the commercially available gibberellin powder is as follows: ; In the formula, The measured mass of commercially available gibberellin powder is in mg. The absolute effective mass concentration of the exogenous gibberellin aqueous solution to be prepared is expressed in mg / L. This is the solvent volume, in liters (L). P represents the purity percentage of commercially available gibberellin powder, with P expressed as a percentage.

[0034] Weigh commercially available gibberellin powder according to the formula for calculating the mass of commercially available gibberellin powder. Dissolve the weighed commercially available gibberellin powder in a partial solvent using conventional experimental preparation methods. Then, use the solvent to prepare an aqueous solution of exogenous gibberellin, in which the gibberellin is gibberellin GA3.

[0035] S42, after being left to stand and drained, the coarse gibberellin seeds without any surface attachments are transferred to the cultivation soaking tank of the soaking module. An exogenous gibberellin aqueous solution with an absolute effective mass concentration of 400 mg / L is injected into the cultivation soaking tank until the exogenous gibberellin aqueous solution with an absolute effective mass concentration of 400 mg / L completely submerges the coarse gibberellin seeds without any surface attachments, and the soaking continues for 24 hours.

[0036] S43, the coarse sandalwood seeds that have been soaked in an exogenous gibberellin aqueous solution with an absolute effective mass concentration of 400 mg / L and have undergone water absorption and swelling with no attached substances on the surface are defined as being in the physiological germination state. After soaking, the coarse sandalwood seeds in the physiological germination state with no attached substances on the surface are transferred from the cultivation soaking tank of the soaking module and filtered dry.

[0037] The S5 provided by this invention includes the following steps in a specific implementation: S51, transfer the physiologically germinating and surface-free coarse rice seed that has been filtered and dried to the inside of the germination module's cultivation tray, and wrap the physiologically germinating and surface-free coarse rice seed in a degreased pure cotton towel that has been sterilized at high temperature and moistened with sterile purified water. The environment in which the cultivation trays are placed is kept at a temperature of 26°C to 30°C, a relative humidity of 85%, and in a dark environment, so that the seeds of the coarse husk tree, which have undergone high-temperature sterilization and are wrapped in a degreased pure cotton towel moistened with sterile purified water, remain in a state of cultivation.

[0038] S52, maintain the coarse bark tree seeds in a physiological germination state with no surface attachments in a light-proof environment with a temperature of 26℃ to 30℃ and a relative humidity of 85%. Check the moisture content of the degreased pure cotton towel that has been sterilized at high temperature and moistened with sterile purified water every 2 to 3 days, and spray sterile purified water on the surface of the degreased pure cotton towel that has been sterilized at high temperature and moistened with sterile purified water. The degreased pure cotton towel, which has undergone high-temperature sterilization and is kept moist with sterile purified water, does not drip when squeezed. After 15 days of cultivation, check the germination status of the coarse husk tree seeds that are in a physiological germination state and have no attached substances on their surface, and pick out any moldy or rotten coarse husk tree seeds.

[0039] S53, continue to maintain the ambient temperature of the incubation tray at 26°C to 30°C and the relative humidity at 85%, and keep it in a dark environment. Replenish sterile purified water at intervals of 2 to 3 days until the cumulative incubation time reaches 30 days. After 30 days of cultivation, the germination status of the coarse rice seed with no attached material on its surface was checked. The coarse rice seed with the radicle tissue penetrating the outer seed coat and showing a white elongation was removed from the high-temperature sterilized and degreased pure cotton towel moistened with sterile purified water and then sown for seedling cultivation.

[0040] To aid in understanding the technical solution of this invention, the following provides an application example and test example of a method for improving the germination of *Phyllostachys edulis*.

[0041] Application Examples: Taking the processing of 10kg of freshly harvested *Symplocos buergeriana* fruit as an example, the 10kg of freshly harvested *Symplocos buergeriana* fruit was laid flat on a carrier with air circulation for dehydration. After 2 days, the outer seed coat of the *Symplocos buergeriana* fruit wrinkled and the surface moisture content decreased. Mechanical force was applied to peel off the outer seed coat of the *Symplocos buergeriana* fruit, and 3.2kg of *Symplocos buergeriana* seeds without any attached substances were obtained. The 3.2kg of *Symplocos buergeriana* seeds without any attached substances were put into a nylon mesh bag with a pore size of 1mm and transferred to a storage module with an operating temperature of 5℃ for continuous storage for 2 months. After the low-temperature storage treatment, the *Symplocos buergeriana* seeds without any attached substances were put into a disinfection module and completely immersed in a 1% potassium permanganate solution for 30 minutes. They were then rinsed with running water for 10 minutes and left to stand in a ventilated place to filter dry until there was no free water attached to the surface. After being allowed to stand and filter dry, the seeds of *Pterocarya stenoptera* with no surface attachments were transferred to a soaking module for treatment with exogenous gibberellin aqueous solution. A commercially available gibberellin powder with a purity of 20% was obtained, and the dosage was calculated. To prepare a 10L volume of 500x exogenous gibberellin aqueous solution, 20g of commercially available gibberellin powder was weighed, dissolved in purified water using standard experimental preparation methods, and then diluted to 10L. The absolute effective mass concentration of gibberellin GA3 in the 10L exogenous gibberellin aqueous solution prepared from 20g of 20% pure commercially available gibberellin powder was 400mg / L. The *Pterocarya stenoptera* seeds with no surface attachments were treated with a 400mg / L 500x exogenous gibberellin aqueous solution. Seeds of *Symplocos buergeriana* that have been soaked in a mycotoxin solution for 24 hours and have swelled with water without any attached material on their surface are defined as seeds of *Symplocos buergeriana* in a physiological germination state without any attached material. These seeds are then wrapped in a degreased pure cotton towel that has been sterilized at high temperature and moistened with sterile purified water, and placed in a germination module cultivation tray in a light-proof environment at 28°C and 85% relative humidity for 30 days. The moisture content of the degreased pure cotton towel that has been sterilized at high temperature and moistened with sterile purified water is checked every 2 to 3 days, and sterile purified water is sprayed onto the surface of the degreased pure cotton towel that has been sterilized at high temperature and moistened with sterile purified water.

[0042] When using commercially available gibberellin powder with a purity percentage of 20%, the absolute effective mass concentration of a 500-fold dilution of exogenous gibberellin aqueous solution is 400 mg / L, the absolute effective mass concentration of a 1000-fold dilution of exogenous gibberellin aqueous solution is 200 mg / L, and the absolute effective mass concentration of a 1500-fold dilution of exogenous gibberellin aqueous solution is approximately 133.3 mg / L.

[0043] Test example: To address the concentration variation during the soaking stage, a water control group and three treatment groups with different concentration gradients were set up. The experimental subjects were *Symplocos lucida* seeds that had undergone low-temperature storage at 5℃ and room temperature storage, followed by soaking in potassium permanganate solution and static drying to ensure a surface free of adhering substances. Eight independent experimental batches were formed, with 800 *Symplocos lucida* seeds of free surface adhering substances extracted from each batch and further divided into eight sample groups. Each sample group consisted of 100 *Symplocos lucida* seeds of free surface adhering substances. The eight sample groups corresponded to the water control group, the 500-fold dilution of exogenous gibberellin aqueous solution treatment group, the 1000-fold dilution of exogenous gibberellin aqueous solution treatment group, and the 1500-fold dilution of exogenous gibberellin aqueous solution treatment group, respectively. The control group was soaked in purified water. The treatment groups with 500x, 1000x, and 1500x exogenous gibberellin solutions were soaked in the corresponding exogenous gibberellin solutions. The purified water used in the control group was the same volume as the exogenous gibberellin solution used in the 500x, 1000x, and 1500x exogenous gibberellin solutions. Both the purified water and the exogenous gibberellin solution completely submerged the seeds of *Symplocos buergeriana* that had no attached substances on the surface. The soaking time for all parallel samples was maintained for 24 hours. After soaking, the coarse cotton tree seeds with no adhering substances on the surface were wrapped in degreased pure cotton towels that had been sterilized at high temperature and moistened with sterile purified water. They were then uniformly transferred to a light-proof environment with a temperature of 28℃ and a relative humidity of 85% for 30 days of constant temperature cultivation. Every 2 to 3 days, the moisture content of the degreased pure cotton towels that had been sterilized at high temperature and moistened with sterile purified water was checked, and sterile purified water was sprayed onto the surface of the degreased pure cotton towels that had been sterilized at high temperature and moistened with sterile purified water. Germination rate was calculated as the percentage of *Paeonia lactiflora* seeds whose radicles penetrated the outer seed coat and showed an elongated, white bud after 30 days of culture, out of 100 seeds in each sample group; moldy and rotten *Paeonia lactiflora* seeds were still calculated as 100 seeds in each sample group; the average radicle length was the average length of the radicle tissue of *Paeonia lactiflora* seeds that showed an elongated, white bud after 30 days of culture, and the data in Table 1 are the average values ​​of data from four independent experimental batches; In this invention, the optimal receptor binding extreme value was determined solely based on the data distribution of germination rate, germination potential, and average radicle length in the water control group, the 500-fold dilution of exogenous gibberellin aqueous solution treatment group, the 1000-fold dilution of exogenous gibberellin aqueous solution treatment group, and the 1500-fold dilution of exogenous gibberellin aqueous solution treatment group. The absolute effective mass concentration of the exogenous gibberellin aqueous solution corresponding to the highest germination rate, germination potential, and average radicle length was determined as the optimal receptor binding extreme value within the tested concentration range. The optimal receptor binding extreme value is used to represent the comprehensive evaluation result of germination rate, germination potential, and average radicle length. The material cost is calculated based on the following: when 100 radicle tissues penetrate the outer seed coat of *Symplocos buergeriana* seeds and exhibit an elongated, white-outward morphology after 30 days of cultivation in the 500x, 1000x, and 1500x exogenous gibberellin aqueous solution treatment groups, the material cost includes the cost of *Symplocos buergeriana* seeds, commercially available gibberellin powder, potassium permanganate solution, purified water, sterile purified water, and degreased pure cotton towels. The material cost includes the cost of *Symplocos buergeriana* seeds, commercially available gibberellin powder, potassium permanganate solution, purified water, sterile purified water, and degreased pure cotton towels, but does not include the cost of operating the storage module and the germination module.

[0044] Based on the germination rate of each treatment group, the amount of *Cephalotaxus fortunei* seeds required to produce 100 seeds with radicle tissue penetrating the outer seed coat and exhibiting an elongated, white-tipped morphology after 30 days of cultivation in each treatment group was calculated. Material costs were also calculated based on the set material costs for *Cephalotaxus fortunei* seeds, commercially available gibberellin powder, potassium permanganate solution, purified water, sterile purified water, and degreased pure cotton towels. The set material costs for each treatment group were calculated proportionally to the number of *Cephalotaxus fortunei* seeds requiring treatment.

[0045] Based on the germination rate of 68% in the 500-fold dilution of exogenous gibberellin aqueous solution treatment group, theoretically 147.06 *Symplocos buergeriana* seeds need to be treated; based on the germination rate of 54% in the 1000-fold dilution of exogenous gibberellin aqueous solution treatment group, theoretically 185.19 seeds need to be treated; and based on the germination rate of 66% in the 1500-fold dilution of exogenous gibberellin aqueous solution treatment group, theoretically 151.52 seeds need to be treated.

[0046] Assume the material cost of each 100 *Pterocarya stenoptera* seeds treated is set at 10.00 yuan, the material cost of potassium permanganate solution is set at 0.40 yuan, the material cost of purified water is set at 0.20 yuan, the material cost of sterile purified water is set at 0.20 yuan, and the material cost of degreased pure cotton towels is set at 1.00 yuan; the material costs of commercially available gibberellin powder produced per 100 *Pterocarya stenoptera* seeds treated with 500x exogenous gibberellin aqueous solution, 1000x exogenous gibberellin aqueous solution, and 1500x exogenous gibberellin aqueous solution are set at 0.10 yuan, 0.05 yuan, and 0.033 yuan, respectively.

[0047] Under the aforementioned material cost conditions, the material cost for obtaining 100 seeds of *Symplocos buergeriana* with radicle tissue penetrating the outer seed coat and exhibiting an elongated, white-tipped morphology after 30 days of culture in the 500-fold exogenous gibberellin aqueous solution treatment group was 17.50 yuan; the material cost for obtaining 100 seeds of *Symplocos buergeriana* with radicle tissue penetrating the outer seed coat and exhibiting an elongated, white-tipped morphology after 30 days of culture in the 1000-fold exogenous gibberellin aqueous solution treatment group was 21.94 yuan; and the material cost for obtaining 1500-fold exogenous gibberellin aqueous solution treatment group was... The material cost of obtaining 100 seeds with radicle tissue penetrating the outer seed coat and exhibiting an elongated, white-outlet morphology after 30 days of culture in the gibberellin aqueous solution treatment group was 17.93 yuan; the material cost of obtaining 100 seeds with radicle tissue penetrating the outer seed coat and exhibiting an elongated, white-outlet morphology after 30 days of culture in the 500-fold exogenous gibberellin aqueous solution treatment group was lower than that of the 1000-fold and 1500-fold exogenous gibberellin aqueous solution treatment groups.

[0048] Table 1. Effects of different concentrations of exogenous gibberellin aqueous solution on the germination index of *Pterocarya stenoptera* seeds.

[0049]

[0050] From Table 1 and Appendix Figure 5 To be continued Figure 8It was found that after a 30-day cultivation period (seeds stored under cold and room temperature), the germination rate of *Pterocarya stenoptera* seeds without surface attachments in the water control group was 0%. The germination rate of *Pterocarya stenoptera* seeds without surface attachments in the 500-fold dilution of exogenous gibberellin aqueous solution (cold storage) treatment group reached 68%, while the germination rate in the room temperature storage treatment group was 38%. The germination rate of the 1000-fold dilution of exogenous gibberellin aqueous solution (cold storage) treatment group was 54%, while the germination rate in the room temperature storage treatment group was 12%. The germination rate of the 1500-fold dilution of exogenous gibberellin aqueous solution (cold storage) treatment group was 66%, while the germination rate in the room temperature storage treatment group was 18.37%. The data distribution shows that the germination rate and average radicle length of the 500-fold dilution of exogenous gibberellin aqueous solution (seeds stored under cold and room temperature) treatment group reached the highest. The absolute effective mass concentration of 400 mg / L was determined to be the optimal extreme value for receptor binding to break dormancy in *Pterocarya stenoptera* seeds without surface attachments within the concentration range of this test, based on the data distribution of germination rate, germination potential, and average radicle length. 400 mg / L + cold treatment is the optimal combination of receptors for breaking dormancy in *Symplocos rubrum* seeds; Under the aforementioned material cost settings, based on the calculated material cost of obtaining 100 radicles penetrating the outer seed coat and exhibiting an elongated, white-tipped radicle after 30 days of cultivation in each treatment group, the treatment group treated with 500 times dilution of exogenous gibberellin aqueous solution had the lowest material cost and the highest overall levels of germination rate, germination potential, and average radicle length. Therefore, 500 times dilution of exogenous gibberellin aqueous solution was established as the optimal treatment parameter within the concentration range tested.

[0051] While specific embodiments of the present invention have been described above, those skilled in the art should understand that these specific embodiments are merely illustrative. Those skilled in the art can omit, substitute, and modify the details of the above methods and systems in various ways without departing from the principles and essence of the present invention. For example, combining the above method steps to perform substantially the same function and achieve substantially the same result using substantially the same method falls within the scope of the present invention.

Claims

1. A method for improving the bud break of *Symplocos buergeriana*, characterized in that the method... include: Harvest the fruits of the *Symplocos buergeriana*, air-dry the fruits and remove the outer seed coat to obtain the seeds of *Symplocos buergeriana*. The seeds of the *Symplocos buergeriana* were stored in a low-temperature environment. The seeds of *Symplocos lucida* were soaked in a potassium permanganate solution after storage to treat the fungi and bacteria attached to the outer seed coat of the seeds. After soaking, the seeds were rinsed with running water and allowed to stand and drain in a ventilated environment. Prepare an exogenous gibberellin aqueous solution, and soak the dried seeds of *Symplocos buergeriana* in the exogenous gibberellin aqueous solution to allow the seeds to absorb water and swell to reach the physiological germination state. After soaking, remove and drain the seeds. The seeds of the *Symplocos lucida* that have undergone high-temperature sterilization and are moistened with sterile purified water are wrapped in a degreased pure cotton towel to reach the physiological germination state and then filtered dry. They are then cultured in the dark under suitable temperature and relative humidity conditions for germination of the seeds. Sterile purified water is sprayed onto the surface of the degreased pure cotton towel to keep the towel moist. When the radicle tissue penetrates the outer seed coat of the *Symplocos buergeriana* seed and shows an elongated, white appearance, the degreased cotton towel is removed from the *Symplocos buergeriana* seed, and the seed is sown for seedling cultivation.

2. The method for improving the budding of *Phyllostachys edulis* according to claim 1, characterized in that, Harvest the fruits of the *Symplocos buergeriana* that have reached physiological maturity and whose outer seed coat exhibits a natural, mature color. The fruits of the *Symplocos lucida* are laid flat in a single layer on a carrier with good air circulation, and exposed in a cool, ventilated environment without direct sunlight. The flowing air removes the moisture evaporated from the surface of the fruits.

3. The method for improving the budding of *Phyllostachys edulis* according to claim 1, characterized in that, When air-drying the fruit of the *Symplocos rubra* tree, the endpoint of the dehydration process is determined based on the surface morphological changes of the outer seed coat of the fruit. When the outer seed coat of the fruit of the *Symplocos buergeriana* shrinks in volume due to water loss, and fine wrinkles form on the surface of the outer seed coat, and the outer seed coat can be peeled off by manual rubbing to separate the *Symplocos buergeriana* seeds without any attached substances on the surface, mechanical or manual rubbing force is applied to the *Symplocos buergeriana* fruit that has reached the end of the dehydration process, causing the outer seed coat of the *Symplocos buergeriana* fruit to break and fall off from the surface of the *Symplocos buergeriana* seeds.

4. The method for improving the bud break of *Phyllostachys edulis* according to claim 1, characterized in that, The seeds of *Symplocos lucida* obtained after removing the outer seed coat of the fruit are placed in a breathable packaging container made of nylon mesh bags, the pore size of which is smaller than the diameter of the seeds. A breathable packaging container is placed inside the storage module. A thermocouple obtains the temperature of the storage module's interior space, and a refrigeration unit uses a compressor to cool the seeds, thus performing low-temperature storage treatment.

5. The method for improving the bud break of *Phyllostachys edulis* according to claim 1, characterized in that, After rinsing with running water, transfer the coarse husk seeds to a well-ventilated environment and let them stand and dry. When the surface of the coarse husk seed loses its reflective luster and no water film adheres between adjacent coarse husk seeds, the static filtration is completed, ensuring that no free water adheres to the outer seed coat surface of the coarse husk seed.

6. The method for improving the bud break of *Phyllostachys edulis* according to claim 1, characterized in that, The preparation of exogenous gibberellin aqueous solution includes: Obtain commercially available gibberellin powder, and calculate the weighing mass of commercially available gibberellin powder based on the absolute effective mass concentration and target volume of the exogenous gibberellin aqueous solution to be prepared, as well as the purity percentage of commercially available gibberellin powder; The commercially available gibberellin powder was weighed and dissolved in a portion of the solvent, and then the volume was adjusted to the target volume using the solvent to prepare an exogenous gibberellin aqueous solution. The gibberellin in the exogenous gibberellin aqueous solution was gibberellin GA3.

7. The method for improving the budding of *Phyllostachys edulis* according to claim 6, characterized in that, After being left to stand and drain, the seeds of the *Symplocos buergeriana* were transferred to the cultivation soaking tank of the soaking module, and an exogenous gibberellin aqueous solution was injected into the tank to completely submerge the seeds.

8. The method for improving the budding of *Phyllostachys edulis* according to claim 1, characterized in that, During the cultivation of *Pterocarya stenoptera* seeds, the moisture content of degreased pure cotton towels that have undergone high-temperature sterilization and are moistened with sterile purified water is checked, and sterile purified water is sprayed onto the surface of the degreased pure cotton towels that have undergone high-temperature sterilization and are moistened with sterile purified water.

9. A method for improving the bud break of *Phyllostachys edulis* according to claim 8, characterized in that, Spray sterile purified water onto the surface of the degreased pure cotton towel that has undergone high-temperature sterilization and is moistened with sterile purified water, so that the degreased pure cotton towel that has undergone high-temperature sterilization and is moistened with sterile purified water remains moist and that no free liquid drips from the degreased pure cotton towel that has undergone high-temperature sterilization and is moistened with sterile purified water when squeezed.

10. The method for improving the budding of *Phyllostachys edulis* according to claim 1, characterized in that, During the cultivation of *Symplocos buergeriana* seeds, the germination status of *Symplocos buergeriana* seeds in a physiological germination state is checked; When moldy or rotten seeds of the coarse husk tree are found, they should be picked out and removed.

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