A rapid bag culture method of wild agaricus blazei murrill fruiting body

Abstract

The application provides a rapid bag material cultivation method of wild agaricus blazei murrill fruit bodies, and belongs to the field of domestication and cultivation of wild rare edible fungi. The rapid cultivation method can realize the appearance of primordium in 5 days and the picking after 2 days of the appearance of the primordium through the specific methods of limiting special culture medium preparation, strain separation and purification, bag material cultivation material preparation, bag material cultivation and special cultivation management. The method has the advantages of simple operation, fast growth and fast fruiting, and has a good application prospect.

Description

Technical Field

[0001] This invention belongs to the field of domestication and cultivation of edible fungi, and in particular relates to a rapid bag cultivation method for the fruiting bodies of wild tea tree mushrooms. Background Technology

[0002] Wild tea tree mushroom ( Cyclocybe aegerita (V. Brig.) Vizzini is a rare edible mushroom, characterized by its delicious taste, crisp texture, rich and pure aroma, and delicate quality. It can be processed into canned tea tree mushrooms, tea tree mushroom soy sauce, tea tree mushroom concentrate, and dried tea tree mushrooms. Tea tree mushrooms are rich in nutrients, containing 18 essential amino acids, especially 8 essential amino acids that the human body cannot synthesize, as well as glucan, fungal protein, carbohydrates, and abundant B vitamins and various minerals, such as iron, potassium, zinc, and selenium, which are higher than in other fungi. Tests show that every 100g of dried product contains 28.9g of protein and a high amino acid content of 25.56g. Traditional Chinese medicine believes that this mushroom has kidney-tonifying, diuretic, lower back pain-relieving, dampness-removing, spleen-strengthening, and diarrhea-stopping effects, making it an ideal food for patients with hypertension, cardiovascular disease, and obesity. Tea tree mushrooms also possess tonic, blood pressure-lowering, calming, and anti-aging properties, and have significant therapeutic effects on frequent urination, edema, asthma, bedwetting in children, arthritis, low-grade fever, and detoxification. Experiments have shown that tea tree mushroom extracts have an inhibition rate of 80%–90% against sarcoma and Ehrlich ascites carcinoma in mice. However, domestic tea tree mushroom production suffers from poor lignin decomposition and low utilization rates. Wild tea tree mushrooms are extremely rare, with limited wild distribution and low yields, and most people are unfamiliar with them, making large-scale production using sawdust difficult. Therefore, finding tea tree mushroom strains with high lignin-decomposing activity and conducting corresponding research and development is a prerequisite for developing and efficiently producing tea tree mushrooms. Summary of the Invention

[0003] In view of this, the purpose of the present invention is to provide a rapid bag cultivation method for wild tea tree mushroom fruiting bodies.

[0004] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a rapid bag cultivation method for wild tea tree mushroom fruiting bodies, comprising the following steps: 1) Boil purple potatoes containing amylose in water at 80-85℃ for 5 minutes. Filter the solution through 4 layers of gauze, then filter the filtrate through 8 layers of gauze, and finally filter it again through 120 mesh gauze to obtain a fine filtrate. 2) The fine filtrate was mixed with β-glucose, L-fructose, freshly squeezed malt juice, magnesium sulfate, potassium dihydrogen phosphate and agar in sequence. After complete dissolution, the volume was adjusted to 1000 mL, dispensed into Erlenmeyer flasks and sterilized. After cooling to ≤50℃, vitamin B1 and folic acid were added in sequence, and the pH was adjusted to 6.5. The mixture was then dispensed into test tubes for later use to obtain the culture medium for special wild tea tree mushrooms. 3) Collect wild tea tree mushrooms growing on the trunks of tea trees over 30 years old, and place the tissue at the junction of the cap and stipe that has no contact with air in a special wild tea tree mushroom culture medium and culture it in the dark at 15~20℃ for 5~6 days to obtain tea tree mushroom strain; 4) Inoculate the tea tree mushroom spawn into the bag cultivation substrate and cultivate the mycelium in the bag substrate for 60 days under the conditions of air temperature 15~20℃, air humidity 65%~70%, center temperature of bag cultivation substrate ≤23℃, and pH 5~6.5 to obtain tea tree mushroom bag substrate mycelium; 5) Place the mycelium of *Agrocybe aegerita* bag substrate under the conditions of air temperature ≤20℃, air humidity ≥85%, center temperature of bag substrate ≤23℃, and pH of bag substrate 5~6.5 for 5~9 days to obtain wild *Agrocybe aegerita* fruiting bodies; The culture medium for each 1000 mL of special wild tea tree mushroom is made from the following components by weight: 200 g of purple potato containing amylose, 10 g of β-glucose, 10 g of L-fructose, 10 mL of freshly squeezed malt extract, 0.5 g of magnesium sulfate, 0.25 g of potassium dihydrogen phosphate, 20 g of agar, 100 mg of vitamin B1, 0.1 g of folic acid, 1000 mL of distilled water, pH 6.5; The formula for the bagged cultivation substrate is as follows: 69% broadleaf tree sawdust, 10% wheat bran, 10% corn cob, 5% corn flour, 4% fermented soybean meal, 1% gypsum, 1% lime, and 60% moisture content.

[0005] Preferably, the air temperature for the growth of wild tea tree mushrooms in step 3) is 15~20℃ and the humidity is 65%~70%.

[0006] This invention provides wild tea tree mushroom fruiting bodies prepared by the rapid bag cultivation method described above.

[0007] Compared with the prior art, the present invention has the following beneficial effects: This invention provides a rapid bag cultivation method for wild tea tree mushroom fruiting bodies, which has the advantages of simple operation and can be used to carry out efficient cultivation using the obtained wild high-activity low-temperature strains that decompose lignin, and has good application prospects. Attached Figure Description

[0008] Figure 1 It is a wild tea tree mushroom; Figure 2The primordia produced by *Agrocybe aegerita* obtained through this invention; Figure 3 The fruiting body of artificially cultivated tea tree mushroom obtained by the present invention. Detailed Implementation

[0009] This invention provides a rapid bag cultivation method for wild tea tree mushroom fruiting bodies, comprising the following steps: 1) Boil purple potatoes containing amylose in water at 80-85℃ for 5 minutes. Filter the solution through 4 layers of gauze, then filter the filtrate through 8 layers of gauze, and finally filter it again through 120 mesh gauze to obtain a fine filtrate. 2) The fine filtrate was mixed with β-glucose, L-fructose, freshly squeezed malt juice, magnesium sulfate, potassium dihydrogen phosphate and agar in sequence. After complete dissolution, the volume was adjusted to 1000 mL, dispensed into Erlenmeyer flasks and sterilized. After cooling to ≤50℃, vitamin B1 and folic acid were added in sequence, and the pH was adjusted to 6.5. The mixture was then dispensed into test tubes for later use to obtain the culture medium for special wild tea tree mushrooms. 3) Collect wild tea tree mushrooms growing on the trunks of tea trees over 30 years old, and place the tissue at the junction of the cap and stipe that has no contact with air in a special wild tea tree mushroom culture medium and culture it in the dark at 15~20℃ for 5~6 days to obtain tea tree mushroom strain; 4) Inoculate the tea tree mushroom spawn into the bag cultivation substrate and cultivate the mycelium in the bag substrate for 60 days under the conditions of air temperature 15~20℃, air humidity 65%~70%, center temperature of bag cultivation substrate ≤23℃, and pH 5~6.5 to obtain tea tree mushroom bag substrate mycelium; 5) Place the mycelium of *Agrocybe aegerita* bag substrate under the conditions of air temperature ≤20℃, air humidity ≥85%, center temperature of bag substrate ≤23℃, and pH of bag substrate 5~6.5 for 5~9 days to obtain wild *Agrocybe aegerita* fruiting bodies; The culture medium for each 1000 mL of special wild tea tree mushroom is made from the following components by weight: 200 g of purple potato containing amylose, 10 g of β-glucose, 10 g of L-fructose, 10 mL of freshly squeezed malt extract, 0.5 g of magnesium sulfate, 0.25 g of potassium dihydrogen phosphate, 20 g of agar, 100 mg of vitamin B1, 0.1 g of folic acid, 1000 mL of distilled water, pH 6.5; The formula for the bagged cultivation substrate is as follows: 69% broadleaf tree sawdust, 10% wheat bran, 10% corn cob, 5% corn flour, 4% fermented soybean meal, 1% gypsum, 1% lime, and 60% moisture content.

[0010] Preferably, the air temperature for the growth of wild tea tree mushrooms in step 3) is 15~20℃ and the humidity is 65%~70%.

[0011] This invention provides wild tea tree mushroom fruiting bodies prepared by the rapid bag cultivation method described above.

[0012] In this invention, purple potatoes containing amylose are boiled in water, filtered through 4 layers of gauze, then filtered through 8 layers of gauze, and finally filtered again through 120-mesh gauze to obtain a fine filtrate; the boiling temperature is preferably 80~85℃, more preferably 81~84℃, and even more preferably 83℃; the boiling time is 5 min.

[0013] In this invention, the fine filtrate is sequentially mixed with β-glucose, L-fructose, freshly squeezed malt extract, magnesium sulfate, potassium dihydrogen phosphate, and agar. After complete dissolution, the volume is adjusted to 1000 mL, dispensed into Erlenmeyer flasks, sterilized, and cooled to ≤50℃. Vitamin B1 and folic acid are then added sequentially, and the pH is adjusted to 6.5. The mixture is then dispensed into test tubes for later use, thus obtaining a culture medium for a special wild tea tree mushroom. The mass ratio of the purple potato containing amylose, β-glucose, L-fructose, malt extract, magnesium sulfate, potassium dihydrogen phosphate, agar, vitamin B1, and folic acid is 200:10:10:10:0.5:0.25:20:0.1:0.1.

[0014] In this invention, wild tea tree mushrooms grown on the trunks of tea trees over 30 years old are collected. The tissue at the junction of the cap and stipe, which is not exposed to air, is placed in a special wild tea tree mushroom culture medium for dark cultivation to obtain tea tree mushroom spawn. The temperature of the dark cultivation is preferably 15~20℃, more preferably 16~19℃, even more preferably 18℃, and the cultivation time is preferably 5~6 days, more preferably 6 days.

[0015] In this invention, *Agrocybe aegerita* spawn is inoculated into bag cultivation substrate, and the substrate is used for mycelial growth to obtain *Agrocybe aegerita* bag cultivation mycelium. The formula of the bag cultivation substrate is: 69% broadleaf tree sawdust, 10% wheat bran, 10% corn cob, 5% corn flour, 4% fermented soybean meal, 1% gypsum, and 1% lime, with a moisture content of 60%. The preferred air temperature for mycelial growth is 15-20℃, more preferably 16-19℃, and even more preferably 18℃. The preferred air humidity for mycelial growth is 65%-70%, more preferably 66%-69%, and even more preferably 68%. The preferred core temperature of the substrate for mycelial growth is ≤23℃. The preferred pH for mycelial growth is 5-6.5, more preferably 5.5-6.2, and even more preferably 6. The preferred incubation time is 60 days.

[0016] In this invention, the mycelium of *Agrocybe aegerita* is cultured in bags to obtain wild *Agrocybe aegerita* fruiting bodies; the preferred air temperature for cultivation is ≤20℃, the preferred air humidity is ≥85%, the core temperature of the bag cultivation medium is ≤23℃, and the preferred pH of the bag cultivation medium is 5~6.5, more preferably 5.5~6.2, and even more preferably 6; the preferred cultivation time is 5~9 days, more preferably 6~8 days, and even more preferably 7 days.

[0017] This invention provides wild tea tree mushroom fruiting bodies prepared by the rapid bag cultivation method described above.

[0018] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0019] Example 1

[0020] Ingredients: 200 g purple potatoes containing amylose, 10 g β-glucose, 10 g L-fructose, 10 mL freshly squeezed malt extract, 0.5 g magnesium sulfate, 0.25 g potassium dihydrogen phosphate, 20 g agar, 0.1 g vitamin B1, 0.1 g folic acid, and 1000 mL distilled water.

[0021] Purple potatoes containing amylose were boiled in distilled water at 80°C for 5 minutes. The solution was first filtered through 4 layers of gauze, then through 8 layers of gauze, and finally through 120-mesh gauze to obtain the filtrate. All other reagents except vitamin B1 and folic acid were added sequentially. After complete dissolution, the solution was brought to a final volume of 1000 mL, dispensed into Erlenmeyer flasks, and sterilized. After sterilization, when the culture medium cooled to below 50°C, vitamin B1 and folic acid were added in a clean bench. The pH of the solution was then adjusted to 6.5 using a precision pH meter and dispensed into test tubes for later use.

[0022] Collect wild tea tree mushrooms (such as those growing on the trunks of 30-year-old tea trees) Figure 1 (As shown): Take tissue from the connection between the cap and the stem that has not been exposed to air and place it in a special culture medium. Incubate at 15-20℃ in the dark for 5-6 days to obtain the tea tree mushroom strain.

[0023] Through multiple rounds of screening using various cultivation formulas, the selected high-efficiency bag cultivation substrate is as follows: 69% broadleaf tree sawdust, 10% wheat bran, 10% corn cob, 5% corn flour, 4% fermented soybean meal, 1% gypsum, and 1% lime, with a moisture content of 60%. The *Agrocybe aegerita* spawn is inoculated into the bag cultivation substrate. The air temperature is 18-20℃, the air humidity is 65-70%, the center temperature of the bag cultivation substrate does not exceed 23℃, and the pH of the bag cultivation substrate is ≤6.5. The substrate is allowed to fully colonize the bags after 60 days of cultivation.

[0024] After the mycelium growth period, the substrate is moved to the fruiting room. The air temperature in the fruiting room is 18℃, the humidity is ≥85%, the center temperature of the substrate bags is ≤23℃, and the pH of the substrate is ≤6.5. Primordia appear on the substrate bags after 5 days in the fruiting room (e.g., ...). Figure 2 As shown), cultivated fruiting bodies can be harvested 2 days after the primordia appear (e.g. Figure 3 (As shown).

[0025] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A rapid bag cultivation method for wild tea tree mushroom fruiting bodies, characterized in that, Includes the following steps: 1) Boil purple potatoes containing amylose in water at 80-85℃ for 5 minutes. Filter the solution through 4 layers of gauze, then filter the filtrate through 8 layers of gauze, and finally filter it again through 120 mesh gauze to obtain a fine filtrate. 2) The fine filtrate was mixed with β-glucose, L-fructose, freshly squeezed malt juice, magnesium sulfate, potassium dihydrogen phosphate and agar in sequence. After complete dissolution, the volume was adjusted to 1000 mL, dispensed into Erlenmeyer flasks and sterilized. After cooling to ≤50℃, vitamin B1 and folic acid were added in sequence, and the pH was adjusted to 6.

5. The mixture was then dispensed into test tubes for later use to obtain the culture medium for special wild tea tree mushrooms. 3) Collect wild tea tree mushrooms growing on the trunks of tea trees over 30 years old, and place the tissue at the junction of the cap and stipe that has no contact with air in a special wild tea tree mushroom culture medium and culture it in the dark at 15~20℃ for 5~6 days to obtain tea tree mushroom strain; 4) Inoculate the tea tree mushroom spawn into the bag cultivation substrate and cultivate the mycelium in the bag substrate for 60 days under the conditions of air temperature 15~20℃, air humidity 65%~70%, center temperature of bag cultivation substrate ≤23℃, and pH 5~6.5 to obtain tea tree mushroom bag substrate mycelium; 5) Place the mycelium of *Agrocybe aegerita* bag substrate under the conditions of air temperature ≤20℃, air humidity ≥85%, center temperature of bag substrate ≤23℃, and pH of bag substrate 5~6.5 for 5~9 days to obtain wild *Agrocybe aegerita* fruiting bodies; The culture medium for each 1000 mL of special wild tea tree mushroom is made from the following components by weight: 200 g of purple potato containing amylose, 10 g of β-glucose, 10 g of L-fructose, 10 mL of freshly squeezed malt extract, 0.5 g of magnesium sulfate, 0.25 g of potassium dihydrogen phosphate, 20 g of agar, 100 mg of vitamin B1, 0.1 g of folic acid, 1000 mL of distilled water, pH 6.5; The formula for the bagged cultivation substrate is as follows: 69% broadleaf tree sawdust, 10% wheat bran, 10% corn cob, 5% corn flour, 4% fermented soybean meal, 1% gypsum, 1% lime, and 60% moisture content.

2. The rapid bag cultivation method according to claim 1, characterized in that, Step 3) The air temperature for the growth of wild tea tree mushrooms is 15~20℃ and the humidity is 65%~70%.

3. Wild tea tree mushroom fruiting bodies prepared by the rapid bag cultivation method according to claim 1 or 2.

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