Method for indoor high-density breeding of phascolosoma esox

Abstract

This invention relates to the field of aquaculture technology, specifically a method for high-density indoor multi-layer rack culture of *Sipunculus nudus*. This method requires a dedicated indoor multi-layer rack high-density culture system, which consists of five parts: a small culture container module, a permeable barrier cloth, a circulating temperature-controlled water tank, a multi-layer load-bearing rack, and an electrically controlled water circuit module. This invention comprehensively solves all the pain points of traditional outdoor seabed aquaculture through a modular and controllable indoor multi-layer rack culture process. The advantages can be verified one by one against the shortcomings of traditional technologies. Specific beneficial effects are explained in the following dimensions: It eliminates the dependence on large areas of tidal flats and seabeds; indoor culture can be carried out simply by mining suitable sandy soil and sediment. Existing tidal flats and ponds can be used to cultivate fish, shrimp, and crabs, achieving composite land use and doubling the overall income for the same tidal flat area. The indoor three-dimensional multi-layer structure enables high-density culture, with a culture density per unit area that is 3-4 times that of traditional open-air seabeds.

Description

Technical Field

[0001] This invention relates to the field of aquaculture technology, specifically to a method for high-density indoor multi-layer rack culture of *Sipunculus nudus*. Background Technology

[0002] Sipunculus worms (Phascolosoma esculenta), belonging to the class Sipunculidae of the phylum Sipunculidae, are commonly known as mud worms, mud stings, or mud slugs. They are a specialty economic aquatic product of the southeastern coastal areas of my country, prized for their delicious meat, rich in amino acids and bioactive proteins. They can be eaten fresh, processed into frozen products, or used to make mud slug jelly. Market demand is stable and increasing year by year. Currently, industrialized aquaculture relies entirely on outdoor mudflats and near-shore seawater ponds converted into sea fields, representing the only mature aquaculture model in the industry. However, large-scale expansion is extremely difficult due to multiple limitations related to site, environment, labor, energy consumption, diseases, and production efficiency.

[0003] Current mainstream traditional sea field aquaculture technology

[0004] Traditional outdoor aquaculture operates in open tidal flats, and the entire operational process has a low degree of standardization. The specific steps are as follows:

[0005] Seabed land preparation and transformation: Large-scale aquaculture blocks are divided into near-shore mudflats. Within each block, regular aquaculture ridges are excavated, with a ridge width controlled at 100-120cm and a ridge height no less than 30cm. Drainage ditches 30-40cm wide are installed between the ridges. Pedestrian and transportation passages are reserved between blocks. The site must be above normal sea level to prevent suffocation and death of sipunculid larvae due to prolonged seawater immersion. The land preparation phase requires the use of excavators to excavate ditches, lay large-diameter PVC drainage pipes, and install 380V outdoor aluminum core cables with high-power pumping equipment, resulting in high costs for civil engineering and electromechanical modifications.

[0006] Seedling release: Natural wild Sipunculus seedlings are artificially collected from the coastal mudflats, with 500-800 catties of seedlings released per mu of seabed. The release window is strictly limited, and can only be carried out in the evening or at night when there is no strong light and the ambient temperature is 22-30℃. Heavy rain must not occur within 1-2 hours after release, as rainwater will dilute the soil salinity, causing the seedlings to absorb too much water, reduce their activity, and die in large numbers because they cannot burrow into the bottom mud. When the ambient temperature is below 15℃, the activity of the seedlings is significantly reduced, and the survival rate after entering the soil drops sharply. Seedlings cannot be released at all during low temperature periods.

[0007] Routine water environment control: Seawater is pumped daily into the furrows based on the dryness and wetness of the tidal flat soil, ensuring the seawater overflows the highest point of the ridges. The temperature and humidity of the bottom sediment are maintained by relying on the open-air natural environment. During the rainy season, heavy rainfall significantly reduces the salinity of the bottom sediment. When the surface salinity of the soil falls below 6‰, it affects the feeding of Sipunculus nudus. Additional high-salinity seawater needs to be pumped for flood irrigation to regulate this. The open-air aquaculture ditches and ridges cover a large area, and the water consumption for a single flood irrigation is extremely high. The continuous operation of the pumping equipment generates huge electricity costs.

[0008] Feeding management: Unfermented semi-dry chicken manure is fed only once a month throughout the entire breeding process. This feed is inexpensive and is the only available raw material for outdoor breeding. In the open environment, strong winds, rainstorms, and typhoons will directly wash away the feed on the ridges, resulting in serious nutrient loss. Rainwater and flooded seawater will continuously wash away the bottom mud, causing continuous loss and volatilization of feed nutrients. The feed distribution on the ridges is uneven, and some breeding areas will suffer from long-term feed shortages.

[0009] Breeding cycle and feeding restrictions: The traditional breeding cycle is as long as 15 to 18 months; Sipunculus nudus can only go out to feed at night when there is dim light, no wind, and no rain. It stops feeding in strong light, high temperature, rain, and strong wind, which greatly reduces the effective feeding time and slows down the growth rate.

[0010] Adult harvesting: After the breeding cycle reaches the target, workers use short-handled hoes to press and dig the soil for harvesting. This requires bending over and working close to the mudflats, which is a high-intensity labor. Using iron tools to dig can easily scratch and pierce the bodies of the crickets. The damage rate is 2% to 3% per 100 kilograms of adults. Damaged individuals lose their commercial value, and additional manual sorting of defective individuals is required after harvesting. Harvesting is entirely dependent on outdoor weather and experienced diggers. Harvesting is not possible on rainy days or when there is a shortage of labor, which cannot meet the market's immediate supply demand.

[0011] The core defects of traditional outdoor seabed aquaculture

[0012] Farming sites are scarce and land utilization is extremely low.

[0013] The stock of compliant nearshore mudflats and convertible seafields continues to shrink, and obtaining approval is extremely difficult. Each seafield can only be used for the single aquaculture of Sipunculus nudus, resulting in a limited land function. The sediment sand content must be strictly higher than 10%; mudflats with insufficient sand content cannot be used for aquaculture. Clogged sediment pores hinder the air release, excretion, and movement of Sipunculus nudus, leading to a significant drop in survival rates. Site conversion requires a large amount of engineering machinery, pipes, and power lines, resulting in substantial upfront infrastructure investment. Outdoor aluminum core cables age and break down due to prolonged exposure to sunlight, and high-power pumping equipment operates continuously with electricity, making the humid environment of the mudflats highly susceptible to electric shock accidents.

[0014] High labor barriers, difficulty in recruiting, and continuously rising labor costs

[0015] Outdoor farming presents multiple harsh working conditions: land preparation, feeding, and harvesting all require skilled farming techniques, resulting in high labor intensity; in summer, the open-air heat and sun exposure, coupled with muddy and slippery mudflats, make workers prone to heatstroke and sprains; prolonged contact with unfermented chicken manure and the strong, pungent odor throughout the work process lead to low worker acceptance; the strenuous, back-bending work of harvesting easily causes lumbar muscle strain, resulting in a serious loss of young labor and a continuously widening gap in the supply of skilled workers.

[0016] The tolerance for error in seedling placement is extremely low, and seedling losses are uncontrollable.

[0017] Seedling release is subject to multiple external environmental constraints such as light, temperature, and rainfall, and the suitable window of opportunity for operation is extremely short; releasing seedlings in low temperature or rainy weather will directly cause large-scale seedling death, and the cost of seedling procurement losses cannot be controlled.

[0018] The feed is heavily contaminated and has extremely low nutrient utilization.

[0019] Unfermented semi-dry chicken manure is directly dumped onto the mudflats. After being washed away by rainwater, the organic matter flows into the nearshore waters, causing eutrophication and pollution of nearshore marine life. Under the combined effects of natural depletion in the open environment and flooding, feed nutrients are severely lost. Uneven feeding leads to differentiation in population growth and inconsistent sizes of farmed animals.

[0020] High water and electricity consumption, resulting in persistently high operating costs.

[0021] The open-air, large-area furrow structure consumes a huge amount of water, requiring daily flood irrigation and continuous pumping for salt regulation during the rainy season, resulting in persistently high water and electricity consumption; outdoor power lines suffer significant losses, leading to high equipment maintenance costs.

[0022] Slow growth rate, long breeding cycle, and low output efficiency

[0023] Limited by natural light and weather, Sipuntia have very little effective feeding time, resulting in low feed nutrient utilization and a complete breeding cycle of 15 to 18 months. The breeding density per unit area of ​​land is low, and the upper limit of output per acre is fixed, making it difficult to improve economic benefits.

[0024] High harvesting losses and poor supply flexibility

[0025] Manual harvesting of ironware results in a 2% to 3% loss of goods, and the sorting process adds extra labor costs. Harvesting is entirely dependent on weather and labor, and cannot be done on demand according to market orders, often missing the sales window and compressing the profit margin of farmers.

[0026] The technical problem to be solved by this invention is that there are defects in the existing technology.

[0027] Addressing all the shortcomings of traditional outdoor aquaculture, this invention completely abandons the open-air tidal flat seafield model and constructs a fully indoor, controllable, multi-layered, three-dimensional, high-density aquaculture system, simultaneously solving the following key technical challenges:

[0028] It provides a standardized bottom mud ratio suitable for the habitat, burrowing, and excretion of Sipunculus nudus, and controls the sand content of the bottom mud to 28% to 35%, plus or minus 3%, solving the problems of insufficient porosity and poor drainage and aeration of traditional tidal flat bottom mud;

[0029] Develop modular support containers, permeable and seepage-proof supporting components, and layered water supply systems adapted to indoor three-dimensional aquaculture to achieve small-volume water circulation and humidity control, and significantly reduce water and electricity consumption;

[0030] We developed an indoor multi-layer vertical racking structure for aquaculture, which enables three-dimensional utilization of space and achieves a farming density of 3-4 times higher than that of traditional sea bass, depending on the size of the seedlings.

[0031] It replaces highly polluting unfermented chicken manure and is paired with specialized organic aquaculture feed, achieving the dual effects of promoting growth and improving bottom sediment, thus eliminating outdoor feed loss and near-shore pollution problems.

[0032] By creating a constant-temperature, light-proof, and controllable indoor environment, the limitation of Sipunculus nudus that only feeds at night is broken, the effective feeding time is extended, and the breeding cycle is shortened;

[0033] Simplify the entire process of seedling release, feeding, and harvesting, reduce the physical and skill requirements for labor, and eliminate harsh working environments such as high temperatures, mud, and odors outdoors.

[0034] It enables rapid harvesting of adult organisms without damage, unaffected by external weather, and can be harvested and supplied at any time according to market demand.

[0035] To achieve the integrated utilization of tidal flats, the original sea ponds and pits can be used for simultaneous aquaculture of fish, shrimp and crabs after the extraction of suitable bottom sediment, thereby increasing the comprehensive benefits per unit of land.

[0036] Therefore, in order to solve the above problems, a method for high-density indoor multi-layer shelf culture of palatable Sipunculus nudus is provided. Summary of the Invention

[0037] The purpose of this invention is to overcome the shortcomings of existing outdoor tidal flat and seabed aquaculture of Sipunculus nudus, such as limited space, high energy consumption, high labor threshold, large loss of seedlings and feed, long growth cycle, severe harvesting damage, limited supply, and environmental pollution. This invention provides a method for high-density indoor multi-layer rack culture of Sipunculus nudus. Through three-dimensional, modular, layered culture, precise bottom sediment ratio, indoor light-proof, constant-temperature water environment control, specialized environmentally friendly feed, and a non-destructive, simple harvesting process, this method achieves large-scale, standardized aquaculture with low energy consumption, low labor, high density, short cycle, zero pollution, and flexible harvesting, while simultaneously improving the comprehensive utilization rate of tidal flat land and the economic benefits of aquaculture.

[0038] A method for high-density indoor multi-layer rack breeding of palatable Sipunculus nudus requires a dedicated indoor multi-layer rack high-density breeding system. The system consists of five parts: a small breeding container module, a water-permeable barrier cloth, a circulating temperature-controlled water tank, a multi-layer load-bearing rack, and an electrically controlled water circuit control module.

[0039] Small-scale aquaculture container modules

[0040] As the supporting unit for the substrate inhabited by Sipunculus nudus, a rectangular structure is preferred to avoid the problem of slow water seepage, water accumulation, and oxygen deficiency in the center of a square container, which can lead to the death of Sipunculus nudus. The net height of the container is ≤14cm, and the four sides and bottom of the container are opened with perforated drainage grids to ensure that the substrate can be quickly permeated with water and does not accumulate water.

[0041] The material is made of food-grade environmentally friendly materials that are resistant to seawater acid and alkali corrosion. Options include 304 stainless steel, PP plastic, and HDPE high-density polyethylene. HDPE is the optimal solution because it is anti-aging, lightweight, and has low processing costs.

[0042] Permeable barrier fabric

[0043] Lay inside the small aquaculture container module, completely covering the four sides and bottom perforated grid, its function is to prevent the loss of bottom mud and sand, restrict the living area of ​​Sipunculus nudus, and at the same time ensure rapid water infiltration and aeration; there are two options: acid and alkali resistant nylon mesh and water-permeable and breathable fiber cloth, with fiber cloth being the preferred option.

[0044] Fiber cloth technical parameters: weight 100~150g; nylon mesh parameters: mesh count ≥10≤20 mesh; all materials meet national aquatic environmental protection standards and will not decompose or release harmful substances when soaked in seawater for a long time.

[0045] Circulating temperature-controlled water bath

[0046] It is used to hold several small aquaculture container modules, and is equipped with an integrated water inlet pipe, overflow pipe, bottom drainage pipe and electric water circuit module; the overall height of the water tank is 2cm higher than the small aquaculture container module, the lower edge of the overflow hole is flush with the top of the container, and the drainage hole is opened at the lowest point of the water tank to realize fully automatic irrigation, overflow water stop and complete drainage.

[0047] Sink materials available: 304 stainless steel, PP, PVC, EPS / EPP foam box, HDPE geomembrane + spliced ​​environmentally friendly multi-layer board. The combination of HDPE geomembrane and environmentally friendly multi-layer board is the optimal solution, as it is low in cost, waterproof and corrosion resistant, and easy to disassemble and expand to create multiple sink combinations.

[0048] Multi-layer load-bearing rack

[0049] The indoor three-dimensional layered load-bearing structure has ≥5 layers of shelves, with a net spacing of 30cm between each layer. After deducting the height of the water tank itself, each layer is reserved with 14cm of operating space for daily observation, feed feeding, and container disassembly and harvesting. The shelf material is made of galvanized steel, cold-rolled steel, or stainless steel. The corresponding thickness of the profile is selected according to the total load-bearing capacity of the breeding scale. The load-bearing capacity of each layer is customized according to the total weight of a single set of containers, bottom mud, and water.

[0050] Electrically controlled water circuit regulation module

[0051] Integrating a water level sensor, temperature probe, solenoid valve, circulating water pump, and control terminal, it automatically completes the entire process of water inlet flooding, overflow water shut-off, timed drainage, and high and low temperature triggered water replenishment and temperature control, without the need for continuous manual monitoring and control of the water environment.

[0052] The core sediment mixing process of this invention

[0053] The ratio of bottom mud and sand is the core technology of this invention, directly determining the efficiency of Sipunculus burrowing, excretion, respiration, and survival rate. Specific ratios and preparation processes are as follows:

[0054] Sediment composition: tidal flat mud + fine sea sand, with fine sand particle size ≤0.5mm, and the overall sediment sand content is controlled within the range of 28% to 35% plus or minus 3%;

[0055] Mix proportion testing method: Choose one of sedimentation stratification method or drying and sieving method to accurately calculate the sand-soil ratio;

[0056] Mixing operation: When the sand-soil ratio is unbalanced, use a concrete mixer to mix thoroughly. After mixing, let it stand to settle and remove excess seawater. Take the settled and moistened mixed bottom mud for later use.

[0057] Substrate filling standard: When filling into small breeding container modules, the net thickness of the substrate should be 10-12cm. The minimum filling thickness should not be less than 10cm. Insufficient thickness will result in insufficient space for Sipunculus nests, while excessive thickness will lead to low soil utilization, inconvenience in excavation and daily operations, increased container costs, slow drainage, and lack of oxygen at the bottom center.

[0058] Complete Operating Method for Indoor Multi-Layer Shelf High-Density Cultivation of Delicious Sipunculid Cassiopeia

[0059] The aquaculture method of this invention consists of six core steps: aquaculture system assembly, bottom mud filling, seedling release, automated control of indoor and aquatic environments, standardized feeding with specialized feed, and adult harvesting. The process parameters, operating procedures, and control standards for each step are as follows:

[0060] Step 1: Layered Assembly of Indoor Aquaculture System

[0061] Multi-layer load-bearing shelves are placed flat in a closed indoor breeding workshop. The workshop is equipped with light-proof, ventilation, and temperature and humidity control equipment to isolate it from outdoor rain, strong light, and extreme temperature interference.

[0062] The circulating temperature-controlled water tanks are arranged in order according to the shelf layers, with a corresponding number of water tanks for each shelf layer, ensuring that the water tanks are placed horizontally without tilting to avoid local water accumulation;

[0063] Cut the permeable barrier fabric to completely cover the four sides and bottom perforated grid of the small aquaculture container module. Lay it flat without wrinkles or exposed grid areas to prevent sand and soil from flowing out through the perforations.

[0064] Connect the electronically controlled water circuit control module to the water tank inlet pipe, overflow pipe, and bottom drain pipe. Debug the water level, temperature sensing, and automatic water pump solenoid valve to confirm that the water inlet, overflow, water stop, and drainage functions are normal.

[0065] Step 2: Standardized sediment loading pretreatment

[0066] Nearshore mud and fine sea sand with a particle size ≤0.5mm were collected, and the sand content was tested by drying and sieving. The mixture was then blended to a sand content of 28% to 35% ±3% by mass.

[0067] The mixer thoroughly mixes the sand and soil mixture, and allows it to settle and filter out excess free seawater, retaining moist and formed bottom mud;

[0068] Fill the mixed bottom mud into the small aquaculture container module that has been laid with a water-permeable barrier cloth, control the bottom mud thickness to 10-12cm, and lightly level the surface after filling without repeatedly compacting it to ensure that the internal pores of the bottom mud are permeable to air and water.

[0069] Step 3: High-density release of seedlings in a controlled environment

[0070] The indoor workshop temperature is pre-controlled and stabilized at 22-30℃. Strong lighting is turned off, and a dim, light-proof indoor environment is maintained, free from wind, rain, and fresh water erosion.

[0071] Healthy, natural, and palatable seedlings of Sipunculus nudus were selected from tidal flats.

[0072] Seedlings are evenly sown on the surface of the bottom mud of a container that has been moistened with seawater. The density of the culture per unit area is 3-4 times that of traditional outdoor seabeds, depending on the size of the seedlings. In an indoor environment with normal temperature and no light, the seedlings can be released at any time of the year without being restricted by low temperature of 15℃, rainfall, or strong light.

[0073] Two hours after the seedlings are released, the injured seedlings will be unable to burrow into the soil, so the seedlings that have not burrowed into the soil need to be picked out. At this time, seedlings can also be replanted, and files should be created and transferred to routine water environment management 3-5 days later.

[0074] Step 4: Fully automated control of indoor temperature, humidity, and water environment

[0075] By relying on an electronically controlled water circuit module to achieve standardized closed-loop regulation, the traditional daily manual flood irrigation mode is replaced, significantly reducing water consumption and electricity costs.

[0076] Temperature Trigger Control: When the indoor ambient temperature is >30℃ or <15℃, the system automatically activates the inlet solenoid valve, artificially insulated seawater is flooded to the surface of the bottom mud to complete cooling, heat preservation, and moisture retention; the system is kept submerged in water for 6-8 hours daily to allow the Sipuncula larvae to absorb nutrients from the water. Water Level and Salinity Control: The overflow hole of the water tank limits the maximum water level, and the water supply automatically stops when the overflow line is reached; the seawater used for aquaculture must have a salinity of ≥6‰≦16‰, as high salinity is not conducive to growth. The workshop is equipped with a seawater mixing and storage tank, which automatically mixes high-salinity seawater to supplement the salinity when it is insufficient.

[0077] Drainage and circulation: The bottom drain valve is activated at a set time every day to drain excess seawater from the tank. The water can be collected, filtered, and recycled for reuse, reducing the total amount of seawater extracted.

[0078] Light control: The breeding workshop should maintain a low-light environment to eliminate the deficiency of feeding crickets caused by strong light and high temperatures, allowing them to feed and grow normally both day and night. Alternatively, the workshop can maintain normal lighting, but the water tanks should be covered with a black film to block light.

[0079] Step 5: Standardized feeding process for specialized organic feed

[0080] Completely replace traditional unfermented dry chicken manure with delicious organic feed specifically for Sipunculus nudus that meets enterprise standard Q / CTAK124-2024. The operating procedures are as follows:

[0081] Feeding cycle: Feed once every 15 to 20 days, replacing the traditional single-month extensive feeding;

[0082] Feeding method: The feed is evenly spread on the surface of the bottom mud. In the enclosed indoor environment, there is no strong wind or heavy rain, so the feed is not lost or damaged naturally. The nutrients of the feed washed off by the flood irrigation water are collected and filtered with the drainage water and recycled, which greatly improves the nutrient utilization rate.

[0083] Feed functions: The special organic feed undergoes full fermentation and harmless treatment, with no foul or irritating odor, and will not pollute water bodies or workshop air; the feed is rich in essential amino acids and trace elements for the growth of Sipunculus nudus, and simultaneously improves the microbial environment of the bottom sediment, preventing the bottom sediment from rotting and turning black, and producing toxic and harmful substances;

[0084] Feeding uniformity: Modular small containers allow for independent feeding, enabling precise control of the amount of feed dispensed per unit, completely solving the problems of uneven feeding and localized feed shortages in traditional sea pan feeding.

[0085] Step Six: Non-destructive and Simple Adult Harvesting Process

[0086] Once the breeding cycle is complete, harvesting can be done at any time without being restricted by weather or labor. The operation is simple and there is no damage from digging with iron tools.

[0087] Disconnect the water supply to the corresponding water tank and remove the entire small aquaculture container module from the water tank.

[0088] Invert the container onto a flat operating platform. The bottom mud completely falls off and is spread out. The bottom mud thickness is only 10-12cm, and the soil layer is loose and without compaction.

[0089] The adult, palatable Sipunculus crickets can be completely separated by manually sifting and spreading the bottom mud. No hoes or iron tools are needed for digging, and there is no risk of scratches or punctures to the body. The product loss rate is close to 0.

[0090] After harvesting, the sand and soil mixture can be recycled and remixed, then filled into containers for reuse in the next batch of aquaculture, and the bottom mud raw material can be reused.

[0091] Supporting methods for the integrated utilization of tidal flats

[0092] This invention eliminates the need for single-species aquaculture on entire tidal flats, achieving dual benefits for the land: near-shore sea fields are selected, and suitable sandy bottom mud is extracted as needed and transported to indoor workshops for multi-layer rack aquaculture. The low-lying pits and ponds formed after extraction are transformed into fish, shrimp and crab polyculture ponds, with the same plot simultaneously producing two types of aquatic products: sipunculids and fish, shrimp and crab, significantly improving the comprehensive economic benefits per unit of tidal flat land.

[0093] The advantages of this invention compared to the prior art

[0094] This invention comprehensively solves all the pain points of traditional outdoor seabed aquaculture through an indoor multi-layered modular and controllable aquaculture process. Its advantages can be verified one by one against the shortcomings of traditional technologies. Specific beneficial effects are explained from different dimensions:

[0095] Site resource utilization efficiency has been greatly improved, and infrastructure investment has been reduced.

[0096] By eliminating the reliance on large areas of tidal flats and sea fields, indoor aquaculture can be carried out simply by mining suitable sandy soil and bottom mud. Existing tidal flats and ponds can be used to raise fish, shrimp and crabs, achieving composite land use and doubling the comprehensive benefits of the same tidal flat area.

[0097] The indoor three-dimensional multi-layer structure enables high-density aquaculture, with a unit plane aquaculture density 3-4 times that of traditional open-air sea fields, and the land area required for the same output is only 1 / 4 of the traditional model.

[0098] There is no need for large-scale trench excavation, laying of large-diameter drainage pipes, or installation of outdoor high-voltage aluminum core cables, saving huge infrastructure costs for heavy machinery land preparation on tidal flats and outdoor power anti-corrosion renovation; indoor wiring is enclosed, eliminating the risk of aging and leakage due to sun exposure, significantly improving the safety of aquaculture.

[0099] The labor threshold has been significantly lowered, and labor costs and labor losses have decreased substantially.

[0100] The entire process requires no professional skills such as farming, land preparation, or digging with hoes, and does not require high-intensity outdoor physical labor. There are no occupational injuries such as summer sun exposure, muddy tidal flats, sprains, heatstroke, rain, or cold wind.

[0101] The enclosed indoor environment eliminates the strong odor from the use of unfermented chicken manure outdoors, the working environment is clean and comfortable, the difficulty of recruitment is greatly reduced, and the supply of young labor is sufficient.

[0102] The harvesting process is simplified, eliminating the need for bending over and digging the soil at high intensity. The soil can be spread out to separate the individual plants, preventing long-term occupational injuries such as lumbar muscle strain. There is no 2% to 3% product loss during harvesting, saving the manual sorting of defective individuals and reducing labor requirements.

[0103] The tolerance for error in seedling placement has been improved, and the seedling mortality rate has been significantly reduced.

[0104] The indoor workshop temperature, light, and rainwater are completely controllable. The suitable environment of 22-30℃ can be maintained stably throughout the year. It is not restricted by low temperature, heavy rain, or strong light, and seedlings can be placed at any time. There is no problem of seedlings absorbing too much water and dying after being exposed to rain. The survival rate of seedlings after being planted in the soil is steadily improved, and the cost of seedling procurement loss is greatly reduced.

[0105] The feed is environmentally friendly and pollution-free, and its nutrient utilization rate is significantly improved.

[0106] Replaces highly polluting unfermented chicken manure; the specially fermented organic feed has no foul odor, will not cause eutrophication of nearshore waters, and eliminates non-point source pollution from aquaculture.

[0107] Indoors, there is no strong wind, typhoon, or rainstorm, so there is no natural loss of feed. Nutrients that fall off during flood irrigation are collected and recycled, eliminating almost all nutrient loss. There are no issues of uneven feeding or localized feed shortages, which allows the Sipunculus larvae to grow rapidly in the early stages.

[0108] Hydropower energy consumption has been significantly reduced, and operating costs have been lowered.

[0109] Traditional open-air seawater irrigation ditches and furrows cover a huge area, resulting in extremely high water consumption per flood irrigation session. This invention features a small modular container with a small water storage capacity per unit. The electrical control system automatically irrigates only when triggered by high or low temperatures. The water can be recycled, filtered, and reused, reducing the total amount of seawater extracted to less than 1 / 10 of the traditional method. The pumping equipment operates during off-peak hours, pumping water to a high-level storage tank and using gravity for flood irrigation. This significantly reduces electricity consumption and costs, while also eliminating power losses from outdoor high-voltage lines, resulting in a substantial reduction in long-term hydropower operating costs.

[0110] Increased growth rate and significantly shortened breeding cycle

[0111] Traditional open-air methods only allow Sipunculus larvae to feed during the dark and windless hours at night, resulting in a very short effective feeding time. In contrast, this invention provides a long-term, low-light, dark indoor environment, allowing Sipunculus larvae to feed normally both day and night, doubling their feed absorption and utilization rate. Experimental data shows that Sipunculus larvae raised using this method for 7 months are equivalent to those raised in traditional seabed farming for 11 months. The complete farming cycle is shortened from 15-18 months to 10-12 months, increasing capital turnover by more than 40% and significantly improving output per unit time.

[0112] Harvesting is flexible and lossless, matching the market's immediate supply needs.

[0113] Harvesting is not restricted by rain, strong winds, or labor shortages. Containers can be taken out for harvesting at any time according to market orders, so there are no issues of supply delays or missing out on sales opportunities.

[0114] The entire process involves excavation without any metal tools, ensuring that the sipunculids' bodies are free from scratches or punctures, resulting in a product integrity rate of over 99%, eliminating 2% to 3% of harvesting losses, and increasing finished product output and farming profits.

[0115] After harvesting, the sandy soil can be recycled and reused, and the raw material can be recycled to continuously reduce the cost of raw material procurement.

[0116] The bottom sediment environment is controllable, and the survival rate of aquaculture is stable and controllable.

[0117] This invention precisely controls the sand content of the bottom mud to 28%–35% ± 3%, and combines it with a double permeable and breathable structure consisting of a perforated container with a grid pattern at the bottom and a permeable barrier cloth. This completely solves the problems of insufficient sand content and blocked bottom mud pores in traditional tidal flats, which cause sipunculid excretion and respiratory obstruction. The bottom mud is less prone to compaction and decay throughout the breeding process, the incidence of diseases is significantly reduced, and the overall survival rate is consistently higher than that of traditional outdoor breeding methods.

[0118] Obviously, based on the above description of the present invention, and according to common technical knowledge and conventional methods in the field, various other modifications, substitutions or alterations can be made without departing from the basic technical concept of the present invention.

[0119] The following detailed embodiments further illustrate the above-described content of the present invention. However, this should not be construed as limiting the scope of the present invention to the following examples. All technologies implemented based on the above-described content of the present invention fall within the scope of the present invention. Attached Figure Description

[0120] Figure 1 This is a schematic diagram of the process flow structure of the present invention. Detailed Implementation

[0121] The present invention is illustrated below with specific embodiments, but these are not intended to limit the scope of the invention. Figure 1 As shown, this describes a method for high-density indoor multi-layer shelf culture of palatable Sipunculid worms.

[0122] Example 1: Large-scale indoor breeding of Sipuncula chinensis (a type of worm) using 5-layer HDPE modular technology.

[0123] Aquaculture system construction: 5-layer cold-rolled steel load-bearing racks are selected, with a net spacing of 30cm between each layer; each layer holds one set of HDPE geomembrane spliced ​​water tanks, each water tank contains 6 rectangular HDPE small aquaculture container modules, with a net height of 14cm and a grid-like perforated structure on all four sides and bottom; the small containers are 620mm long * 425mm wide * 150mm high, with 150g permeable fiber cloth completely covering the grid inside; the entire water system is equipped with electronically controlled water level and temperature sensing modules.

[0124] Preparation of bottom sediment ratio: Take near-shore mud and fine sea sand less than 0.5mm, dry and sieve to adjust the sand content to about 30-35%, mix thoroughly with a mixer, and filter out excess seawater by sedimentation; fill into a container with a bottom sediment thickness of 11cm.

[0125] Seedling release: Indoor environment of 22-30℃, low light and shade, evenly release healthy seedlings that have been naturally dug and selected, with a release rate of 3-4 times that of traditional seabeds per unit area; 3 days after release, lightly flood with seawater, with a salinity of 12-18‰, and automatically control the water environment: automatically irrigate and keep warm when the temperature is above 30℃ or below 15℃; automatically drain the wastewater from the water tank after soaking in water for 6-8 hours every noon, and recycle the seawater after coarse filtration; maintain a low light environment in the workshop for a long time.

[0126] Feeding Management: Feed the animal once every 15-20 days with Q / CTAK124-2024 standard special organic feed, spread evenly on the surface of the bottom mud, and dissolve it in water to wash away any lost nutrients for unified recycling.

[0127] Harvesting process: After 7 months of culture, remove the container and invert it on the worktable. Spread out 11cm of loose bottom mud and completely separate the adult Sipunculus larvae without any physical damage. The finished product has about 120 larvae per pound, and the growth indicators are equivalent to the growth state of traditional sea field culture at about 11-12 months.

[0128] Example 2: Simple Aquaculture Scheme in a Small-Scale Experimental Workshop Made of 304 Stainless Steel

[0129] System assembly: 3-layer stainless steel rack, with a single layer for placing a stainless steel water tank. Inside the water tank are 6 small 304 stainless steel aquaculture containers, each measuring 600mm long * 400mm wide * 150mm high. The four sides and bottom are perforated with a grid pattern. The interior is lined with 20-mesh salt-resistant nylon mesh as a water-permeable barrier layer. It is equipped with a simple electrically controlled temperature-controlled water supply system.

[0130] Bottom mud preparation: Mix marine mud with fine sand smaller than 0.5mm, with a sand content of about 28-32%, and fill to a thickness of 12cm.

[0131] Aquaculture management: The indoor temperature is 22-30℃, and seedlings can be released all year round; feed with special fermented organic feed every 15-20 days; only in extreme high and low temperatures is the water replenishment and humidity control automatically maintained, and the water consumption is only about 30% of that of an outdoor sea field of the same area.

[0132] Aquaculture Results: Harvesting occurs after a 12-month aquaculture cycle. The finished product is undamaged, yielding approximately 90 birds per jin (500g). Each small module contains about 600-700 birds (approximately 300-300g) of seedlings (2 jin each), resulting in a yield of about 6 jin. Four modules cover 0.96 square meters, yielding a total of 23.3 jin. The average seedling-to-growth ratio is 1:2.9, representing a 30% increase in yield per square meter compared to traditional tidal flat aquaculture. Traditionally, approximately 1000 jin of seedlings are sown per mu (667 square meters), yielding 1800-2100 jin per mu. In practice, 450-500 square meters are used per mu, with approximately 2.2 jin of seedlings per square meter, resulting in a yield of about 4.6 jin per square meter, a growth ratio of 1:2.1. Water and electricity operating costs are significantly reduced.

[0133] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0134] The above description is merely a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made using the contents of the present invention's specification and drawings under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

[0135] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for high-density indoor multi-layer shelf culture of palatable Sipunculus nudus, characterized in that, Includes the following steps: S1: Construct an indoor multi-layer vertical aquaculture system, which includes multi-layer load-bearing shelves, a circulating temperature-controlled water tank, small aquaculture container modules, a water-permeable barrier cloth, and an electrically controlled water circuit module; the multi-layer shelves have ≥5 layers, with a net spacing of 30cm between each layer; the small aquaculture container modules are rectangular, with a net height >12cm and ≤14cm, and perforated drainage grilles are installed on the four sides and bottom; the water-permeable barrier cloth is laid inside the container to cover the perforations, and is selected from 100-150g water-permeable fiber cloth or 10-30 mesh nylon net; the circulating temperature-controlled water tank is 2cm higher than the small container modules, and is equipped with an overflow pipe, a bottom drain pipe, and is connected to the electrically controlled water circuit module; S2: Preparation of suitable aquaculture bottom sediment: Take tidal flat mud and mix it with fine sea sand with a particle size ≤0.5mm. Control the sand content of the bottom sediment to be 28%~35% ±3%. Stir, settle and filter out free seawater. Fill the mixed bottom sediment into a small aquaculture container lined with a permeable barrier cloth. The bottom sediment thickness is 10~12cm. S3: Seedling release: In a closed room with a temperature controlled at 22-30℃ and low light conditions, release the seedlings of Sipunculus nudus evenly. The density of the seedlings per unit area is 3-4 times that of traditional outdoor seabed. Two hours after release, pick out the seedlings that have not burrowed in and re-sow according to the number of damaged seedlings. After 3-5 days (seedling adaptation period), fill the seawater with a salinity of 10-15 degrees. S4: Fully automatic water environment management: The electronically controlled water circuit module detects the indoor temperature. When the temperature is >30℃ or <15℃, it automatically fills the water tank for temperature control. The water tank is automatically stopped when the overflow line is reached. The water tank wastewater is drained daily. The water is recycled after simple coarse filtration. The workshop maintains a low light or dark environment for a long time. S5: Standardized feeding: Feed the insects with delicious organic fermented feed that meets the Q / CTAK124-2024 standard every 15-20 days. The feed is free from rain and strong winds and the nutrients in the feed that dissolves in water are recycled. S6: Non-destructive harvesting: After the breeding cycle reaches the target, the small breeding containers are removed from the water tank and inverted on the operating platform. The bottom mud is spread out, and the adult palatable Sipunculid worms are separated by light sieving. The sand and soil mixture after harvesting can be recycled for the next batch of breeding.

2. The method for high-density indoor multi-layer shelf culture of palatable Sipunculus nudus according to claim 1, characterized in that, The small-scale aquaculture container modules are made of HDPE, PP or 304 stainless steel; the circulating temperature-controlled water tank is preferably made of HDPE geomembrane combined with environmentally friendly multi-layer board splicing.

3. The method for high-density indoor multi-layer shelf culture of palatable Sipunculus nudus according to claim 1, characterized in that, The bottom mud has fine sand particles with a diameter of ≤0.5mm and a thickness of 10-12CM. The sand and soil are mixed using a concrete mixer, and the sand content is tested by drying and screening or sedimentation and stratification.

4. The method for high-density indoor multi-layer shelf culture of palatable Sipunculus nudus according to claim 1, characterized in that, Supporting integrated utilization technology of tidal flats: Extract suitable sandy soil and bottom mud and transport it to indoor aquaculture. The tidal flats and ponds formed after extraction are transformed for polyculture of fish, shrimp and crab.

5. The method for high-density indoor multi-layer shelf culture of palatable Sipunculus nudus according to claim 1, characterized in that, The breeding cycle is 10-12 months, which is more than 40% shorter than the traditional outdoor sea-field breeding method under the same growth size; the harvested finished products are free from mechanical damage and the commodity loss rate is close to 0.

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