Method for breeding of lecanium corni and diaspidiotus spp. and separation device

Abstract

The application discloses a rearing method and a separation device for Leptomastix eponis and citrus psylla, and belongs to the technical field of biological control of agricultural pests. The rearing method comprises four steps: S1, pretreating tender shoots of Litsea cubeba; S2, expanding the citrus psylla with the tender shoots of Litsea cubeba; S3, introducing the Leptomastix eponis for parasitic rearing; and S4, treating the mixed insects by using a hot air type separation device, preferably with a hot air temperature of 40-50 DEG C, a wind speed of 12.5-13 m / s, an action distance of 4 cm, and a single treatment time of 10 s. Under the working condition, the adult psylla driving-off rate is greater than or equal to 95%, the parasitized psylla nymph retention rate is greater than or equal to 90%, and the separation device is matched. The application realizes the full-process standardized and quantitative rearing of the Leptomastix eponis, replaces manual sorting with automatic hot air separation, greatly reduces the production cost, reduces the parasitic pupa damage, improves the finished product purity and the large-scale output efficiency of the Leptomastix eponis, and can provide complete industrialization technical support for the green control of the citrus psylla, a medium insect of citrus Huanglongbing.

Description

Technical Field

[0001] This invention belongs to the field of biological control technology for agricultural pests, specifically relating to a breeding method and separation device for *Tectus glazei* and *Citrus psyllid*. Background Technology

[0002] Citrus Huanglongbing (HLB) is a devastating citrus disease caused by Bacillus phloem. It is mainly spread by the Asian citrus psyllid. There is no effective cure after the disease occurs, which will cause citrus trees to decline year by year and lose their yield. It is a major threat to the global citrus industry.

[0003] Currently, the control of citrus psyllids in orchards relies heavily on chemical pesticides. Long-term use of these pesticides can easily lead to problems such as pesticide resistance in psyllids, excessive pesticide residues in agricultural products, and damage to field biological communities. *Tamarixiaradiata*, a bright-bellied wasp, is a specific parasitic natural enemy of citrus psyllid nymphs. The female wasp can kill psyllid nymphs through both parasitism and feeding. It has a strong ability to colonize in the field and a rapid population growth rate, making it the optimal natural enemy resource for the biological control of citrus psyllids.

[0004] The existing large-scale breeding system for the bright-bellied glazed wasp faces three major technological bottlenecks:

[0005] There are no standardized quantitative parameters for the breeding process: there is no optimal range for the grading of tender shoots of Murraya paniculata, the male-female ratio of psyllids, and the release ratio of wasps and insects. The breeding density of psyllid nymphs is low, the parasitism efficiency of bright-bellied glaze wasps is unstable, and the output of artificial breeding fluctuates greatly.

[0006] The separation of insects relies entirely on manual sorting: After the emergence of the bright-bellied glaze wasp, a large number of unparasitized citrus psyllid adults are mixed in. Manual sorting under a microscope is time-consuming and labor-intensive, and the purity of the natural enemy product is low, which greatly increases the labor cost of large-scale production.

[0007] Lack of dedicated non-destructive automated separation equipment: Conventional airflow screening equipment relies solely on wind speed differences to separate insects, which cannot distinguish between parasitized nymphs attached to tender shoots and active adult psyllids. Airflow impact can easily damage parasitized pupae, significantly reducing subsequent emergence rates. Existing equipment lacks a closed-loop temperature control system, making it impossible to utilize the biological characteristics of adult psyllids escaping high-temperature stress to achieve differentiated separation.

[0008] The lack of a standardized breeding process and a complete set of integrated technologies for hot air non-destructive automatic separation restricts the industrial-scale supply of bright-bellied glazed wasps and makes it difficult to meet the needs of green pest control in large-scale citrus orchards. Summary of the Invention

[0009] To address the shortcomings of existing technologies, such as the lack of standardized quantitative parameters for breeding *Tetranychus sclerotiorum*, unreasonable ratios of host shoots and insects, low rearing density of psyllid nymphs, and low emergence efficiency of *Tetranychus sclerotiorum* parasitizing nymphs; high costs of manual sorting of insects; insufficient purity of natural enemy products; incompatibility with continuous mass production in industrial settings; and the fact that conventional separation equipment relies solely on wind speed screening, easily damaging psyllid nymphs with parasitized pupae and resulting in significant wasp losses, as well as the lack of a dedicated separation device integrating PID closed-loop constant temperature and controllable hot air timing, making it impossible to precisely regulate hot air stimulation conditions for non-destructive separation, this invention provides a breeding method and separation device for *Tetranychus sclerotiorum* and citrus psyllids.

[0010] To achieve the above objectives, the present invention adopts the following technical solution:

[0011] A method for breeding *Tectus glazei* and *Citrus psyllid* includes the following steps:

[0012] S1: Pretreatment of host shoots

[0013] Select tender shoots of Murraya paniculata, remove rotten, discolored and inferior tender shoots, and obtain tender shoots of host plant for propagation;

[0014] S2: Citrus psyllid breeding

[0015] Pretreated Murraya paniculata shoots were inoculated with citrus psyllids for rearing and breeding. The breeding environment was adjusted to cultivate a population of citrus psyllid nymphs of appropriate age.

[0016] S3: Bright-bellied glazed wasp parasitic breeding

[0017] Adjust the breeding environment conditions, take the citrus psyllid nymphs obtained from S2 as the host, and inoculate them with the parasitic brood wasp to obtain a mixed insect body. The mixed insect body includes adult brood wasp, residual adult citrus psyllids, and parasitized psyllid nymphs attached to tender shoots.

[0018] S4: Separation

[0019] The mixed insect bodies obtained from S3 were subjected to hot air treatment using a separation device. Taking advantage of the biological differences between adult citrus psyllids actively escaping when stimulated by hot air and nymphs attached to the tender shoots of Murraya paniculata, the remaining adult citrus psyllids were driven away, and purified bright-bellied glaze wasp products were obtained.

[0020] Furthermore, in step S2, the input ratio of Murraya paniculata shoots to citrus psyllids and the male-female ratio of citrus psyllids are adjusted to cultivate 4-5 instar nymphs of the psyllids; the input mass ratio of shoots to citrus psyllids is 1:15 to 1:25, the male-female ratio of citrus psyllids is 1:2, and the breeding uses V2 or V3 grade Murraya paniculata shoots.

[0021] Furthermore, in step S3, the ratio of male to female *Tetranychus glazei* and the ratio of *Tetranychus glazei* to citrus psyllid nymphs are adjusted to reduce intraspecific competition and improve parasitism efficiency; the ratio of male to female *Tetranychus glazei* is 1:1, and the ratio of *Tetranychus glazei* to citrus psyllid nymphs is 1:8 to 1:16.

[0022] Furthermore, step S4 hot air treatment includes hot air temperature, wind speed, operating distance, and treatment time; the hot air temperature is 40-50℃, the wind speed is 12.5-13m / s, the operating distance is 4cm, and the treatment time is 10s.

[0023] Furthermore, the breeding environment conditions described in steps S2 and S3 are: temperature 24–26°C, relative humidity 67.5%–70%, and photoperiod L:D = 14h:10h.

[0024] The present invention also provides a device for separating the bright-bellied glaze wasp from the citrus psyllid, suitable for performing step S4, including a box body, the front wall of which is provided with a constant temperature heating mechanism, a collection net connected to the left end of the box body for collecting the blown-away adult citrus psyllids; a slide rail is provided at the right end of the box body, and a slider is provided on the slide rail, with a fan running through the slider for generating directional airflow and guiding it to the separation chamber; a cover is movably fitted at the top of the box body, and a placement groove is provided at the bottom of the inner side of the box body for placing the insect-infested tender shoots.

[0025] Furthermore, the constant temperature heating mechanism includes a PID temperature controller and a solid-state relay fixedly mounted on the front wall of the chamber, as well as a dual-tube infrared lamp located on the upper inner side of the chamber; the PID temperature controller is equipped with a temperature probe, which is arranged on the inner wall of the chamber and located below the dual-tube infrared lamp.

[0026] The PID temperature controller, solid-state relay, and dual-tube infrared lamp are connected in series electrically. The PID temperature controller is connected to mains power. The PID temperature controller is electrically connected to the control terminal of the solid-state relay via a low-voltage signal line. The PID temperature controller outputs a control signal to drive the solid-state relay, thereby adjusting the heating power of the dual-tube infrared lamp. The temperature probe collects the temperature inside the chamber in real time and feeds it back to the PID temperature controller, forming a closed-loop constant temperature control structure.

[0027] The beneficial effects of this invention are as follows: This invention limits the optimal range for the grade of Murraya paniculata shoots, the male-female ratio of psyllids, and the release of wasps. When feeding female psyllids with V2 / V3 shoots, the proportion of ovaries at or above level 4 is ≥60%, resulting in a stable high-density production of 4-5 instar parasitic hosts. By rationally controlling the release density of the bright-bellied wasp, intraspecific competition is alleviated, and the average number of parasitic wasps per wasp is stabilized at 7 or more, improving the overall wasp production efficiency by more than 40%. The quantitative parameters of the entire process are replicable, eliminating the reliance on artificial breeding experience.

[0028] Under standard operating conditions of 40–50℃, 12.5–13 m / s, 4 cm, and 10 s, the repulsion rate of adult citrus psyllids is ≥95%, and the retention rate of parasitized psyllid nymphs is ≥90%. Automated hot air treatment completely eliminates the need for manual microscope sorting, reduces the processing time per batch by 90%, lowers labor production costs by 75%, and significantly improves the purity of natural enemy products.

[0029] This invention uses temperature as the core stimulating factor and wind speed as an auxiliary separation factor to drive away only active adult psyllids; the parasitized psyllid nymphs firmly attach to tender shoots, and the short 10-second hot air will not cause the parasitized pupae to die, thus solving the defects of traditional pure airflow screening that impacts and damages bee pupae and reduces bee production.

[0030] The separation device integrates a PID closed-loop temperature control structure, which can automatically and stably maintain the hot air temperature range. With the fan timing control and a fixed 4cm action distance, the hot air stimulation conditions are uniform and controllable. The equipment has a simple structure and is suitable for continuous batch operation in factories.

[0031] This invention provides a complete industrialization solution from host cultivation, psyllid propagation, parasitism by parasitoid wasps to insect purification. It enables the large-scale production of the bright-bellied glaze wasp natural enemy product, which, after being released in the field, continuously suppresses the citrus psyllid population, reduces the application of chemical pesticides, protects the orchard ecology, and reduces the risk of pesticide residues. Attached Figure Description

[0032] Figure 1 This is a grading diagram of different grades of Murraya paniculata shoots according to the present invention;

[0033] Figure 2 This is a grading diagram of the ovaries of different citrus psyllids according to the present invention;

[0034] Figure 3 Bar chart showing the percentage of ovarian development of citrus psyllids on different grades of Murraya paniculata shoots according to the present invention;

[0035] Figure 4 Line graph showing the parasitism of 4th and 5th instar psyllid nymphs by different densities of the present invention.

[0036] Figure 5 This is a graph showing the linear relationship between the hot air contact distance and the output wind speed in this invention.

[0037] Figure 6 This is a graph showing the linear relationship between the hot air action distance and the hot air temperature inside the cavity in this invention.

[0038] Figure 7 This is a bar chart comparing the detachment time of citrus psyllids under different hot air distances according to the present invention.

[0039] Figure 8 This is a statistical chart showing the probability of repelling psyllids with different insect populations at a standard effective distance of 4cm according to the present invention.

[0040] Figure 9 This is a schematic diagram of the overall three-dimensional structure of the separation device of the present invention;

[0041] Figure 10 This is a schematic diagram of the internal structure of the separation device of the present invention;

[0042] Figure 11 These are experimental data on temperature, treatment time, and the distribution of citrus psyllids in this invention.

[0043] Legend:

[0044] 1-Box body, 2-Constant temperature heating mechanism, 201-PID temperature controller, 202-Solid state relay, 203-Temperature probe, 204-Dual-tube infrared lamp, 3-Collection net, 4-Lid, 5-Slider, 6-Fan, 7-Slide rail, 8-Placement slot. Detailed Implementation

[0045] The following embodiments further illustrate the content of the present invention. These embodiments are not intended to limit the scope of protection of the present invention. Any modifications or substitutions made to the methods, steps, or conditions of the present invention without departing from the essence of the technical solution of the present invention are all within the scope of the present invention.

[0046] Unless otherwise specified, the equipment, apparatus and processes used in the embodiments are all conventional equipment, apparatus and processes well known to those skilled in the art.

[0047] The present invention will be further described in detail below with reference to the accompanying drawings, experimental data, and embodiments, but the present invention is not limited to the following embodiments. Unless otherwise specified, the experimental environment is uniformly as follows: indoor temperature 25℃, relative humidity RH 70%, and photoperiod L:D = 14h:10h.

[0048] I. Preliminary Experiments: Gradient Exploration Experiments of Various Process Parameters

[0049] Experiment 1: Effects of the grade of Murraya paniculata shoots on the ovarian development of citrus psyllids

[0050] like Figure 1 The young shoots of Murraya paniculata are divided into six grades, V1-V6, according to their growth status, and the ovarian development of female citrus psyllids is divided into six grades, I-VI.

[0051] like Figure 2 The newly emerged citrus psyllids were mated on old leaves at a female-to-male ratio of 1:2 for 7 days, and then fed on different grades of tender shoots for 5 days. Ovarian development was then analyzed by dissection. Results are shown in […]. Figure 3 .

[0052] The results showed that over 60% of female psyllids feeding on V1 and V2 tender shoots had ovaries at or above grade 4 development; the higher the maturity of the tender shoots, the weaker the development-promoting effect, while females fed on old leaves basically did not develop ovaries.

[0053] Considering the growth of tender shoots and the duration of feeding tolerance, V2 and V3 grade tender shoots are preferred for large-scale breeding.

[0054] Experiment 2: Effect of placement density of *Microcera glazei* on the number of parasites per wasp

[0055] Use 9cm petri dishes lined with moist filter paper to maintain humidity. Place branches of Murraya paniculata containing 50 4th and 5th instar psyllid nymphs each. Introduce 1-5 bright-bellied glazed wasps into each dish. After 24 hours of rearing, count the parasitism. See the results below. Figure 4 .

[0056] Conclusion: As the density of parasitic wasps increases, the parasitism of a single wasp continuously decreases, indicating intraspecific competition. At the same wasp density, the parasitism of 5th instar psyllid nymphs is higher than that of 4th instar nymphs, and 4th-5th instar nymphs are preferred for breeding.

[0057] Experiment 3: Correlation between hot air application distance and wind speed and temperature

[0058] Using the hot air separation device of this invention, the slider 5 is moved by the slide rail 7 to measure the wind speed and temperature corresponding to different air outlet distances, and a linear equation is fitted:

[0059] Wind speed fitting: Y = -0.2350X + 13.62, R² = 0.9986 Figure 5 );

[0060] Temperature fitting: Y = -1.030X + 52.14, R² = 0.8205 Figure 6 );

[0061] The closer the distance, the higher the wind speed and temperature will be. The intensity of the hot air stimulation can be precisely controlled by adjusting the distance.

[0062] Experiment 4: Effect of hot air distance on the escape time of psyllids

[0063] Multiple distance gradients of 4cm, 6cm, 8cm, 10cm, and 12cm were set up. The experiment was repeated 3 times for a single psyllid, and the time of detachment from the tender shoot was recorded. Figure 7 ).

[0064] The results, indicated by uppercase letters, represent significant differences at the 0.01 level: the 4cm group had the shortest detachment time, significantly better than the other distances, and was the optimal effective distance of this invention.

[0065] Experiment 5: Repelling effect of different insect population densities at a standard distance of 4cm

[0066] 10, 20, and 40 adult psyllids were inoculated onto 20cm Murraya paniculata seedlings, respectively. The seedlings were treated with hot air at 4cm and 10s, and the repellency probability was calculated. Figure 8 ).

[0067] Regardless of the insect population size, the probability of psyllids detaching after stimulation is >90%, and a distance of 4cm is suitable for different breeding populations.

[0068] Experiment 6: Temperature Gradient Displacement Verification (Gear Temperature Test)

[0069] An infrared heating gradient device was used with three power levels. The temperature and the number of psyllids in zone A (hot air inlet) and zone B (collection net end) were recorded every 5 minutes. The experimental data are shown in the table below:

[0070] Power level Time (min) Temperature (°C) Number of individuals in area A Number of individuals in area B First Tier 0 21.5 40 0 First Tier 5 33.0 24 16 First Tier 10 37.5 20 20 First Tier 15 39.2 12 28 First Tier 20 39.4 16 24 Second Tier 0 23.7 34 6 Second Tier 5 41.3 10 30 Second Tier 10 45.5 6 34 Second Tier 15 46.2 2 38 Second Tier 20 49.3 0 40 Third Tier 0 23.7 40 0 Third Tier 5 36.2 8 32 Third Tier 10 47.2 6 34 Third Tier 15 48.6 8 32 Third Tier 20 51.4 2 38

[0071] In the table, area A is the hot air input end for placing tender shoots and psyllids, and area B is the adult insect collection side; when the temperature is <35℃, very few psyllids escape, and in the 40-50℃ range, a large number of psyllids escape to area B, thus determining the core stimulation temperature range of hot air to be 40-50℃.

[0072] Example: Figure 9-10 As shown, a device for separating the bright-bellied glaze wasp from the citrus psyllid includes a box 1. The front wall of the box 1 is equipped with a constant temperature heating mechanism 2. The left end of the box 1 is connected to a collection net 3, which is used to collect the blown-away adult citrus psyllids. The right end of the box 1 is equipped with a slide rail 7, which is matched with a slider 5. A fan 6 is installed through the slider 5 to generate directional airflow and guide it to the separation chamber. The top of the box 1 is movably fitted with a cover 4. The bottom inner side of the box 1 is provided with a placement groove 8 for placing tender shoots with insects.

[0073] The constant temperature heating mechanism 2 includes a PID temperature controller 201 and a solid-state relay 202 fixedly mounted on the front wall of the chamber 1, and a dual-tube infrared lamp 204 located on the upper inner side of the chamber 1. The PID temperature controller 201 is equipped with a temperature probe 203, which is located on the inner side wall of the chamber 1 and below the dual-tube infrared lamp 204. The PID temperature controller 201, the solid-state relay 202, and the dual-tube infrared lamp 204 are connected in series and electrically. The PID temperature controller 201 is connected to the mains power. The PID temperature controller 201 is electrically connected to the control terminal of the solid-state relay 202 through a low-voltage signal line. The PID temperature controller 201 outputs a control signal to drive the solid-state relay 202, thereby adjusting the heating power of the dual-tube infrared lamp 204. The temperature probe 203 collects the temperature inside the chamber in real time and feeds it back to the PID temperature controller 201, forming a closed-loop constant temperature control structure.

[0074] The fan outputs directional hot air, and adult psyllids are carried by the airflow into the detachable collection net on the left end. Nymphs with parasitic pupae are attached to tender shoots and left in the placement trough, achieving non-destructive separation.

[0075] Example 1

[0076] S1 Host Shoot Pretreatment: Select V3 grade Murraya paniculata shoots for use;

[0077] S2 Citrus Psyllid Breeding: V3 tender shoots were fed to psyllids at a ratio of 1:20, and the female-to-male ratio of psyllids was 1:2. High density of 4th-5th instar nymphs was obtained by rearing in a standard environment.

[0078] S3 Bright-bellied Glazed Wasp Breeding: Female wasps 1:1, wasp to nymph ratio 1:10, parasitized and raised until adult emergence, to obtain mixed insect bodies;

[0079] S4 hot air separation: adopts Figure 9 , Figure 10 The separation device has an operating distance of 4cm, a hot air temperature of 45℃, a wind speed of 12.8m / s, and a single treatment time of 10s.

[0080] Test results: The removal rate of adult psyllids was 97.2%, the retention rate of parasitized nymphs was 93.5%, and the purity of the finished product of the bright-bellied glaze wasp was 96.8%.

[0081] Example 2

[0082] S2 ratio 1:15, S3 bee-insect ratio 1:8, hot air 40℃, wind speed 12.5m / s; psyllid repulsion rate 95.1%, nymph retention rate 90.2%.

[0083] Example 3

[0084] S2 ratio 1:25, S3 bee-insect ratio 1:16, hot air 50℃, wind speed 13m / s; psyllid repulsion rate 96.5%, nymph retention rate 91.4%.

[0085] Comparative Example 1

[0086] Traditional manual sorting was used, and the breeding scale was exactly the same as in Example 1. Adult citrus psyllids were manually picked out using a stereomicroscope.

[0087] Experimental results: The sorting time per batch was 12 times that of the hot air separation process of this invention, the labor cost increased by 78%, the purity of the finished product with bright glaze wasps was only 72.3%, the parasitic pupae were damaged by contact during the sorting process, and the subsequent output of wasps decreased by 32%.

[0088] Comparative Example 2

[0089] Only room temperature airflow separation is performed, without a constant temperature heating mechanism. The PID constant temperature heating mechanism of the separation device is removed, and only 13m / s room temperature airflow is used for 10s. The other conditions are the same as in Example 1.

[0090] Experimental results: The repulsion rate of citrus psyllid adults was only 41.6%, and a large number of psyllid adults were attached to tender shoots and could not detach, failing to meet the purity requirements for commercial natural enemies.

[0091] Comparative Example 3

[0092] The hot air contact distance deviated by 4cm, and the distance between the hot air outlet and the insect was adjusted to 8cm. All other standard operating conditions remained unchanged.

[0093] Experimental results: The separation time of adult psyllids was significantly prolonged, and the separation probability was only 68.4%, which did not meet the requirements of large-scale production.

[0094] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any modifications, equivalent substitutions, and improvements made by those skilled in the art within the scope of the technology disclosed in the present invention, and within the spirit and principles of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A method for breeding *Microceras glazei* and *Citrus psyllid*, characterized in that, Specifically, the following steps are included: S1: Pretreatment of host shoots Select tender shoots of Murraya paniculata, remove rotten, discolored and inferior tender shoots, and obtain tender shoots of host plant for propagation; S2: Citrus psyllid breeding Pretreated Murraya paniculata shoots were inoculated with citrus psyllids for rearing and breeding. The breeding environment was adjusted to cultivate a population of citrus psyllid nymphs of appropriate age. S3: Bright-bellied glazed wasp parasitic breeding Adjust the breeding environment conditions, take the citrus psyllid nymphs obtained from S2 as the host, and inoculate them with the parasitic brood wasp to obtain a mixed insect body. The mixed insect body includes adult brood wasp, residual adult citrus psyllids, and parasitized psyllid nymphs attached to tender shoots. S4: Separation The mixed insect bodies obtained from S3 were subjected to hot air treatment using a separation device. Taking advantage of the biological differences between adult citrus psyllids actively escaping when stimulated by hot air and nymphs attached to the tender shoots of Murraya paniculata, the remaining adult citrus psyllids were driven away, and purified bright-bellied glaze wasp products were obtained.

2. The method for breeding *Microceras glazei* and *Citrus psyllid* according to claim 1, characterized in that... In step S2, the input ratio of Murraya paniculata shoots to citrus psyllids and the male-female ratio of citrus psyllids are adjusted to cultivate 4-5 instar nymphs of the psyllids. The input mass ratio of shoots to citrus psyllids is 1:15 to 1:25, and the male-female ratio of citrus psyllids is 1:

2. V2 or V3 grade Murraya paniculata shoots are used for breeding.

3. The method for breeding *Microceras glazei* and *Citrus psyllid* according to claim 1, characterized in that... In step S3, the ratio of male to female *Tetranychus glazei* and the ratio of *Tetranychus glazei* to citrus psyllid nymphs are adjusted to reduce intraspecific competition and improve parasitism efficiency. The ratio of male to female *Tetranychus glazei* is 1:1, and the ratio of *Tetranychus glazei* to citrus psyllid nymphs is 1:8 to 1:

16.

4. The method for breeding *Microceras glazei* and *Citrus psyllid* according to claim 1, characterized in that... Step S4, hot air treatment, includes hot air temperature, wind speed, operating distance, and treatment time; the hot air temperature is 40-50℃, the wind speed is 12.5-13m / s, the operating distance is 4cm, and the treatment time is 10s.

5. The method for breeding *Microceras glazei* and *Citrus psyllid* according to claim 1, characterized in that... The breeding environment conditions described in steps S2 and S3 are: temperature 24-26℃, relative humidity 67.5%-70%, and photoperiod L:D = 14h:10h.

6. A device for separating the bright-bellied glazed wasp from the citrus psyllid, suitable for performing step S4, characterized in that... The system includes a box (1), the front wall of which is equipped with a constant temperature heating mechanism (2), and a collection net (3) connected to the left end of the box (1). The collection net (3) is used to collect adult citrus psyllids that have been blown away. The right end of the box (1) is equipped with a slide rail (7), and the slide rail (7) is equipped with a slider (5). The slider (5) is equipped with a slider. A fan (6) is installed throughout to generate directional airflow and guide it to the separation chamber. A cover (4) is movably fitted on the top of the box (1). A placement slot (8) for placing insect-infested tender shoots is provided on the bottom inner side of the box (1).

7. The device for separating the bright-bellied glazed wasp and the citrus psyllid according to claim 6, characterized in that... The constant temperature heating mechanism (2) includes a PID temperature controller (201) and a solid-state relay (202) fixedly installed on the front wall of the box (1), and a double-tube infrared lamp (204) installed on the upper inner side of the box (1); the PID temperature controller (201) is equipped with a temperature probe (203), which is arranged on the inner side wall of the box (1) and located below the double-tube infrared lamp (204); The PID temperature controller (201), solid-state relay (202), and dual-tube infrared lamp (204) are connected in series and electrically. The PID temperature controller (201) is connected to the mains power. The PID temperature controller (201) is electrically connected to the control terminal of the solid-state relay (202) through a low-voltage signal line. The PID temperature controller (201) outputs a control signal to drive the solid-state relay (202) to adjust the heating power of the dual-tube infrared lamp (204). The temperature probe (203) collects the temperature inside the box in real time and feeds it back to the PID temperature controller (201) to form a closed-loop constant temperature control structure.

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