4-allylanisole in the application of preventing and controlling the forest damage of twelve-toothed bark beetle, repellent and preparation method and application thereof

Abstract

The present application relates to the technical field of green control of twelve-toothed bark beetle, and particularly relates to application of 4-allyl anisole in preventing and controlling twelve-toothed bark beetle forest damage, repellent and preparation method and application thereof. The present application finds that 4-allyl anisole has obvious repellent effect on twelve-toothed bark beetle, and 4-allyl anisole can be used as a repellent for twelve-toothed bark beetle, and is used in production to repel twelve-toothed bark beetle and protect the pine forest ecosystem. Meanwhile, the compound selected by the present application is easy to obtain and is a plant-derived volatile substance, has the advantages of good repellent effect, stability and environment-friendly type, and also has wide industrialization prospect.

Description

Technical Field

[0001] This invention relates to the field of green control technology for bark beetle, and in particular to the application of 4-allyl anisole in controlling bark beetle damage in forests, its repellent, preparation method, and application. Background Technology

[0002] The twelve-toothed bark beetle (Ips sexdentatus Boern), belonging to the genus Ips in the family Scolytinae of the order Coleoptera, is an important trunk-boring pest of pine forests in my country. It is distributed in several provinces in China, including Heilongjiang, Jilin, Liaoning, Hebei, Shaanxi, Sichuan, and Yunnan, and is widely distributed in pine forests throughout Eurasia. Unlike most other bark beetles, the adult twelve-toothed bark beetle can directly invade healthy, standing trees, primarily damaging the thick bark at the base of the trunk. Damage significantly weakens the tree, creating conditions for secondary parasitism by other bark beetles and accelerating the tree's death. In its habitat in my country, the twelve-toothed bark beetle typically has two generations per year, overwintering as larvae or adults in the phloem at the base of the trunk or roots. In recent years, the population density of the twelve-toothed bark beetle has increased significantly in Heilongjiang, Jilin, Hebei and other regions of my country, posing a serious threat to Korean pine, Chinese pine, larch and many other pine species.

[0003] Currently, the control of adult bark beetles mainly relies on techniques such as removing infested wood and using attractants. Bark beetle attractants are commercially available, with their main components being bark beetle diene alcohol, bark beetle enol, and cis-verbenol. These are primarily imported and have become a common technique used at the grassroots level. However, bark beetles mainly damage standing trees, and relying solely on attractants is insufficient to control their infestation of pine trees. Currently, the main approach to this type of bark beetle is to use repellents on host plants to block their attack. To date, there is almost no information on the composition of repellents for bark beetles, severely hindering the development of bark beetle pest management techniques using chemochemicals. Therefore, there is a need to screen for bark beetle repellents with repellent activity and stable effects.

[0004] In summary, the repellents and application technologies for the bark beetle (Bark Borer truncatella) are still immature, and simply relying on attractants cannot completely prevent bark beetle infestation of pine trees. Numerous studies both domestically and internationally have shown that some non-host plants contain volatiles with strong repellent behavior against bark beetles, which is the main source of bark beetle repellents. Guided by this repellent screening strategy, this invention screens substances with repellent activity against the bark beetle from some common and inexpensive non-pine volatiles, thereby preparing a repellent for the bark beetle and developing its application technology. The aim is to repel or interfere with the aggregation and host plant attack behavior of the bark beetle, and to achieve combined use with attractants, providing new technical support for the control of the bark beetle and improving the integrated pest management system for the bark beetle. Summary of the Invention

[0005] To address the aforementioned problems, this invention provides the application of 4-allyl anisole in controlling damage caused by the bark beetle (Bark beetle twelfthii) in forests, a repellent agent, its preparation method, and its application. This repellent is used to antagonize the population aggregation of the bark beetle in forests. The repellent can be fixed to the trunk of pine trees, effectively inhibiting the aggregation and damage caused by the bark beetle, thereby protecting the host pine trees from harm.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] This invention provides the application of 4-allyl anisole in controlling damage caused by Bark beetle in forests.

[0008] Preferably, the 4-allyl anisole repels the dodecanotic bark beetle.

[0009] The present invention also provides a repellent for repelling the dodecanotic bark beetle, the repellent containing 1-8 g of 4-allyl anisole.

[0010] Preferably, the repellent contains 4g of 4-allyl anisole.

[0011] The present invention also provides a slow-release bag for repelling the dodecanotic bark beetle, comprising a slow-release carrier, medical gauze placed inside the slow-release carrier, and 4-allyl anisole adsorbed on the medical gauze;

[0012] The content of the 4-allyl anisole is 1~8g.

[0013] Preferably, the content of the 4-allyl anisole is 4g.

[0014] Preferably, each sustained-release bag contains 2g of Chinese medicine gauze;

[0015] The slow-release carrier is a polyethylene plastic bag; the polyethylene plastic bag is low-density polyethylene with a mass percentage of 99%, a thickness of 16 mils, a length of 80 mm, and a width of 60 mm.

[0016] The present invention also provides a method for preparing the sustained-release bag described in the above technical solution, comprising the following steps: adding the 4-allyl anisole to medical gauze, then placing it in a sustained-release carrier and sealing it.

[0017] The present invention also provides the application of the slow-release bag described in the above technical solution in controlling the damage caused by the twelve-toothed bark beetle in forests.

[0018] Preferably, the slow-release bag is fixed at a height of 1m at the base of the pine tree trunk, with one slow-release bag hanging on each affected pine tree.

[0019] The beneficial effects of this invention are:

[0020] (1) Since the slow-release bag of the bark beetle repellent of the present invention has high repellent biological activity, it can be used in healthy forests to repel low-density bark beetles inside the forest and prevent them from accumulating and causing harm on pine trees.

[0021] (2) Used to deter the attack of the dodecathed bark beetle on its host. The repellent of the present invention has highly efficient repellent activity and has a significant antagonistic effect on attractants with host localization function. Its use in forests can reduce the attack and infection of pine trees by the dodecathed bark beetle.

[0022] (3) It can be used in combination with attractants to form a "push-pull" control, which can meet the protection needs of key forest areas in production.

[0023] The bark beetle repellent provided by this invention uses medical gauze and 16-filament black polyethylene plastic bags as carriers, making it easy to process and produce at a low cost. The repellent components can be stably released on the slow-release carrier. This repellent is a highly effective bark beetle repellent, and this invention is an environmentally friendly green biotechnology that can be used to repel the aggregation and invasion of the bark beetle, thereby protecting pine forests. The repellent has been tested at the Hongshi Forestry Bureau in Huadian City, Jilin Province, verifying its good repellent activity and stability. This bark beetle repellent has broad prospects for industrialization. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly introduced below.

[0025] Figure 1 The results of a comparative study on the repellency behavior of various repellents against the bark beetle in June 2025;

[0026] Figure 2 The study aimed to investigate the inhibitory effects of different doses of 4-allyl anisole on the bark beetle in June 2025.

[0027] Figure 3 The effect of 4-allyl anisole on the invasion behavior of Bartholinae dodecanoides in June 2026;

[0028] Figure 4 The results of a comparative experiment on the repellency behavior of 4-allyl anisole against the spruce bark beetle;

[0029] Figure 5 The results of a comparative experiment on the repellency behavior of 4-allyl anisole against the larch bark beetle are presented. Detailed Implementation

[0030] This invention provides the application of 4-allyl anisole in controlling the damage caused by the bark beetle (Bark beetle twelfthii) in forests. In this invention, the 4-allyl anisole repels the bark beetle. The source and purity (mass %) of the 4-allyl anisole in this invention are as follows: 4-allyl anisole (CAS: 140-67-0, Shanghai Maclean Biochemical Technology Co., Ltd., 97%).

[0031] The present invention also provides a repellent for the bark beetle (Bark beetle), said repellent containing 1-8 g of 4-allyl anisole. In the present invention, the repellent preferably contains 4 g of 4-allyl anisole.

[0032] This invention also provides a slow-release bag for repelling the dodecanotic bark beetle, comprising a slow-release carrier, medical gauze placed inside the slow-release carrier, and 4-allyl anisole adsorbed on the medical gauze; the content of the 4-allyl anisole is 1-8g. In this invention, the content of the 4-allyl anisole is preferably 4g. In this invention, the content of medical gauze in each slow-release bag is preferably 2g. In this invention, the slow-release carrier is preferably a polyethylene plastic bag. In this invention, the polyethylene plastic bag is preferably low-density polyethylene with a mass percentage of 99%, a thickness of 16 mils, a length of 80mm, and a width of 60mm.

[0033] The present invention also provides a method for preparing the sustained-release bag described in the above technical solution, comprising the following steps: adding the 4-allyl anisole to medical gauze, then placing it in a sustained-release carrier and sealing it.

[0034] This invention also provides the application of the slow-release bag described in the above-mentioned technical solution in controlling the damage caused by the twelve-toothed bark beetle in forests. In this invention, the slow-release bag is fixed at a height of 1m at the base of the pine trunk, with one slow-release bag hanging on each affected pine tree.

[0035] Reagent source:

[0036] 4-Allyl anisole (CAS: 140-67-0, Shanghai Maclean Biochemical Technology Co., Ltd., 97%), with both chemical purity and isomer purity greater than 95%;

[0037] cis-2-hexenol (CAS: 928-94-9, Shanghai Yuanye Biotechnology Co., Ltd., 93%)

[0038] Eucalyptol (CAS: 470-82-6, Shanghai Maclean Biochemical Technology Co., Ltd., 99.5%).

[0039] Cedarene (CAS: 11028-42-5, Shanghai Maclean Biochemical Technology Co., Ltd., 95%)

[0040] Rhodomycene (CAS: 470-40-6, Sigma-Aldrich, 97%)

[0041] Trans-2-hexenyl acetate (CAS: 2497-18-9, Shanghai Maclean Biochemical Technology Co., Ltd., 99.8%).

[0042] 1-Penten-3-ol (CAS: 616-25-1, Shanghai Maclean Biochemical Technology Co., Ltd., 98%)

[0043] Peperyl alcohol (CAS: 14314-21-7, Shanghai Youkemo Technology Co., Ltd., 95%)

[0044] Betelgebinol (CAS: 14434-41-4, Shanghai Youkemo Technology Co., Ltd., 95%)

[0045] cis-verbenol (CAS: 18881-04-4, Shanghai Youkemo Technology Co., Ltd., 95%)

[0046] 2-Methyl-3-buten-2-ol (CAS: 115-18-4, Shanghai Yuanye Biotechnology Co., Ltd., 98%).

[0047] To further illustrate the present invention, the following detailed description is provided in conjunction with embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0048] Example 1

[0049] Comparative results of experiments on the repellency behavior of various plant volatile components against the bark beetle (Bark beetle duodenale).

[0050] The attractant for the twelve-toothed bark beetle in this embodiment mainly consists of bark beetle enol, bark beetle dienol, and cis-verbenol. The bark beetle repellent is prepared using commercially available cis-2-hexenol, eucalyptol, cedrene, thujone, trans-2-hexenyl acetate, 4-allyl anisole, and 1-penten-3-ol.

[0051] The following eight processes are configured according to the testing requirements.

[0052] Treatment 1: 100 mg of barbiturol, 30 mg of barbiterenol, and 50 mg of cis-verbenol;

[0053] Treatment 2: 100 mg of barbiturol, 30 mg of barbiturol, 50 mg of cis-verbenol, and 4 g of cis-2-hexenol;

[0054] Treatment 3: 100 mg of barbiturol, 30 mg of barbiterol, 50 mg of cis-verbenol, and 4 g of eucalyptol;

[0055] Treatment 4: 100 mg of barbiturol, 30 mg of barbiterenol, 50 mg of cis-verbenol, and 4 g of cedrene;

[0056] Treatment 5: 100 mg of barbiturol, 30 mg of barbiturol, 50 mg of cis-verbenol, and 4 g of basilene;

[0057] Treatment 6: 100 mg of barbiturol, 30 mg of barbiturol, 50 mg of cis-verbenol, and 4 g of trans-2-hexenyl acetate;

[0058] Treatment 7: 100 mg of barbiturol, 30 mg of barbiturol, 50 mg of cis-verbenol, and 4 g of 4-allyl anisole;

[0059] Treatment 8: 100 mg of barbiturol, 30 mg of barbiturol, 50 mg of cis-verbenol, and 4 g of 1-penten-3-ol;

[0060] (1) Bark beetle alcohol, bark beetle diene alcohol, and cis-verbenol were added dropwise to a medical gauze (2g) carrier according to the above usage amounts, and then a bark beetle attractant was prepared. Cis-2-hexenol, eucalyptol, cedrene, thujone, trans-2-hexenyl acetate, 4-allyl anisole, and 1-penten-3-ol were added dropwise to a medical gauze (2g) carrier according to the above usage amounts, and then a sustained-release repellent was prepared.

[0061] (2) Preparation and storage of attractants and repellents: The attractant and repellent components were dripped onto a medical gauze carrier, then placed in a 16-mil black polyethylene plastic bag, and sealed with a sealing machine to obtain attractant and repellent sustained-release bags respectively; the polyethylene plastic bag was made of low-density polyethylene with a mass percentage of 99%, a thickness of 16 mil, a length of 80 mm, and a width of 60 mm. The prepared attractant and repellent sustained-release bags were sealed with aluminum foil and refrigerated at 4℃.

[0062] (3) Field application of repellents:

[0063] a) Experiment location: Hongshi Forestry Bureau, Huadian City, Jilin Province.

[0064] b) Construction of the trap: The trap mainly consists of a funnel-shaped bark beetle trap, purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large and small plates are crossed to form a cross shape (500mm high, 300mm wide), connected to the top cover via a hook at the top, and connected to the funnel via a hook at the bottom. The flared opening for connecting the insect collection bottle is fitted over the lower part of the funnel and connected to the collection bottle.

[0065] c) Hanging position of the repellent: Hang the drug combination in the middle position 20cm from the top of the funnel-shaped bark beetle trap. The drug combination consists of one attractant and one repellent for the bark beetle (open the aluminum foil before use to get the attractant and repellent slow-release bags).

[0066] d) Field setup of the traps: Three replicates were set up for each experimental treatment. The traps were set up at the edge of the Korean pine forest, with a spacing of about 100m between the traps. Two 3m long and 0.5cm thick wires were connected between the two trees, and the traps were fixed in the middle of the wires. The insect collection bottle of the trap was 1m off the ground.

[0067] e) Data collection and trap emptying: Each treatment in this experiment was replicated three times, and the number of bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of bark beetles in the collection bottles was observed every 7 days. After counting, the collected bark beetles were placed in a plastic box and taken to the laboratory.

[0068] The application of the above-described specific steps for repelling the bark beetle was carried out. From June 1st to June 25th, 2025, bark beetle attractants (treatment 1) were used as a positive control. Specific results are shown in Table 1 and [Table data would be inserted here]. Figure 1 The traps were spaced 100m apart, and the collection bottles were approximately 1m off the ground. Each treatment was replicated three times, and the number of bark beetles captured in each treatment was the average of the three traps. The number of bark beetles in the collection bottles was observed every 7 days, depending on weather conditions.

[0069] Table 1. Comparison of the repellency behavior of various repellents on the bark beetle (Bark beetle duodenale).

[0070]

[0071] From Table 1 and Figure 1 The results show that, based on the insect-attracting effect analysis, the control treatment (treatment 1) has an attracting activity against *Bartholinae*, while treatment 7 has a good repellent effect. Treatments 2, 3, 4, 5, and 6 have almost no effect on the number of *Bartholinae* attracted compared to treatment 1, while treatment 8 actually has a synergistic effect on the number of *Bartholinae* attracted. This experiment demonstrates that the *Bartholinae* attractant combined with 4-allyl anisole (treatment 7) can reduce the number of attracted insects, indicating that 4-allyl anisole is an antagonist of the *Bartholinae* attractant.

[0072] Example 2

[0073] Inhibitory effect of different doses of 4-allyl anisole on bark beetle (Bark beetle dodecanoides)

[0074] The following five treatments are prepared according to the testing requirements:

[0075] Treatment 1: 100 mg of barbiturol, 30 mg of barbiterenol, and 50 mg of cis-verbenol;

[0076] Treatment 2: 100 mg of barbiturol, 30 mg of barbiturol, 50 mg of cis-verbenol, and 1 g of 4-allyl anisole;

[0077] Treatment 3: 100 mg of barbiturol, 30 mg of barbiterenol, 50 mg of cis-verbenol, and 2 g of 4-allyl anisole;

[0078] Treatment 4: 100 mg of barbiturol, 30 mg of barbiturol, 50 mg of cis-verbenol, and 4 g of 4-allyl anisole;

[0079] Treatment 5: 100 mg of barbiturol, 30 mg of barbiterenol, 50 mg of cis-verbenol, and 8 g of 4-allyl anisole;

[0080] The attractant for the dodecanotic bark beetle is the same as in Example 1.

[0081] (1) Bark beetle alcohol, bark beetle diene alcohol and cis-verbenol were added dropwise to a medical gauze (2g) carrier according to the above usage amounts, and then a bark beetle attractant was prepared. 4-Allyl anisole was added dropwise to a medical gauze (2g) carrier according to the above usage amounts, and then a sustained-release repellent was prepared.

[0082] (2) Preparation and storage of the repellent: The repellent component was dripped onto a medical gauze carrier (same as in Example 1), then placed in a 16-filament black polyethylene plastic bag, and sealed with a sealing machine to obtain a repellent sustained-release bag. The prepared repellent sustained-release bag was sealed with aluminum foil and refrigerated at 4°C.

[0083] (3) Field application of repellents:

[0084] a) Experiment location: Hongshi Forestry Bureau, Huadian City, Jilin Province.

[0085] b) Construction of the trap: The trap mainly consists of a funnel-shaped bark beetle trap, purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large and small plates are crossed to form a cross shape (500mm high, 300mm wide), connected to the top cover via a hook at the top, and connected to the funnel via a hook at the bottom. The flared opening for connecting the insect collection bottle is fitted over the lower part of the funnel and connected to the collection bottle.

[0086] c) Hanging position of the repellent: The drug combination is hung in the middle position 20cm from the top of the funnel-shaped bark beetle trap. The drug combination consists of one bark beetle attractant (purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd.) and one repellent.

[0087] d) Field setup of the traps: Three replicates were set up for each experimental treatment. The traps were set up at the edge of the Korean pine forest, with a spacing of about 100m between the traps. Two 3m long and 0.5cm thick wires were connected between the two trees, and the traps were fixed in the middle of the wires. The insect collection bottle of the trap was 1m off the ground.

[0088] e) Data collection and trap emptying: Each treatment in this experiment was replicated three times, and the number of bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of bark beetles in the collection bottles was observed every 7 days. After counting, the collected bark beetles were placed in a plastic box and taken to the laboratory.

[0089] The application of the above-described specific steps for repelling the bark beetle (Bark Beetle) was carried out. From June 10th to July 5th, 2025, a plant-derived attractant for the bark beetle (Treatment 1) was used as a positive control. Specific results are shown in [link to results]. Figure 2 The traps were spaced 100m apart, and the collection bottles were approximately 1m off the ground. Each treatment was replicated three times, and the number of bark beetles captured in each treatment was the average of the three traps. The number of bark beetles in the collection bottles was observed every 7 days, depending on weather conditions.

[0090] Table 2. Effects of different doses of 4-allyl anisole on the attraction of Bark beetle dodecanoides.

[0091]

[0092] Analysis of insect-attracting effects (e.g.) Figure 2As shown in Table 2, treatment 1 (commercial attractant) has a good attraction effect on Bartholin's dodecanotic beetle. When slow-release bags prepared with different amounts of 4-allyl anisole were used in combination with the attractant, it was found that the number of Bartholin's dodecanotic beetles trapped gradually decreased with increasing amounts of 4-allyl anisole. Treatments 4 and 5 significantly reduced the number of Bartholin's dodecanotic beetles trapped, but the difference between the two was not significant. From the perspective of repellency and economic cost, 4g of 4-allyl anisole can have a significant repellency effect on the aggregation behavior of Bartholin's dodecanotic beetle in the field.

[0093] Example 3

[0094] Effects of 4-allyl anisole on the invasion behavior of Bark beetle dodecanoides

[0095] The following two treatments are prepared according to the test requirements:

[0096] Process 1: Blank handling;

[0097] Treatment 2: 4g of 4-allyl anisole;

[0098] (1) Prepare 4-allyl anisole according to the above requirements to obtain the repellent.

[0099] (2) Preparation and storage of the repellent: The repellent component was dripped onto a medical gauze carrier (same as in Example 1), then placed in a 16-filament black polyethylene plastic bag, and sealed with a sealing machine to obtain a repellent sustained-release bag. The prepared repellent sustained-release bag was sealed with aluminum foil and refrigerated at 4°C.

[0100] (3) Field application of repellents:

[0101] a) Experiment time and location: Hongshi Forestry Bureau, Huadian City, Jilin Province (May 10 - June 10, 2026);

[0102] b) Bait wood trapping: Select red pine trees that have just begun to be invaded by bark beetles as bait wood to lure the bark beetles.

[0103] c) Hanging position of the repellent: Fix the repellent slow-release bag 1m from the base of the trunk of the affected red pine tree with thin wire, and hang one repellent slow-release bag for each affected pine tree.

[0104] d) The entry pores of the twelve-toothed bark beetle before the statistical experiment.

[0105] e) Field survey of repellency effect: Each treatment was replicated three times in this experiment. Two months later, the number of entry holes of the 12-toothed bark beetle on each wood segment was counted.

[0106] Table 3. Effects of 4-allyl anisole on the invasion of Bartholinae (dodecanoides).

[0107]

[0108] From the invasion holes on the trunks of red pine trees in each treatment group ( Figure 3 (See Table 3). Statistical analysis revealed an average of 28.67 new invasion pores per affected tree segment without repellent. When the repellent slow-release bag was fixed to the trunk, the invasion of the bark beetle into the bait tree was significantly reduced, with only 5.67 invasion pores per segment in treatment 2 (4-allyl anisole). This experiment demonstrates that, under field conditions, a repellent prepared from 4-allyl anisole can reduce the infestation of Korean pine trunks by the bark beetle.

[0109] Comparative Example 1

[0110] Comparative results of tests on the repellency behavior of 4-allyl anisole against the spruce bark beetle.

[0111] The attractant for the spruce bark beetle in this embodiment mainly consists of 2-methyl-3-buten-2-ol and cis-verbenol, purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The repellent for the spruce bark beetle was prepared using commercially available 4-allyl anisole.

[0112] The following two treatments are prepared according to the test requirements.

[0113] Treatment 1: 4g of 2-methyl-3-buten-2-ol, 100mg of cis-verbenol;

[0114] Treatment 2: 4 g of 2-methyl-3-buten-2-ol, 100 mg of cis-verbenol, and 4 g of 4-allyl anisole;

[0115] (1) 2-Methyl-3-buten-2-ol and cis-verbenol were added dropwise to a medical gauze (2g) carrier according to the above usage amount, and then a spruce bark beetle attractant was prepared. 4-Allyl anisole was added dropwise to a medical gauze (2g) carrier according to the above usage amount, and then a sustained-release repellent was prepared.

[0116] (2) Preparation and storage of attractants and repellents: The attractant and repellent components were dripped onto a medical gauze carrier, then placed in a 16-mil black polyethylene plastic bag, and sealed with a sealing machine to obtain attractant and repellent sustained-release bags; the polyethylene plastic bag was made of low-density polyethylene with a mass percentage of 99%, a thickness of 16 mil, a length of 80 mm, and a width of 60 mm. The prepared attractant and repellent sustained-release bags were sealed with aluminum foil and refrigerated at 4°C.

[0117] (3) Field application of repellents:

[0118] a) Experimental location: Jingouling Forest Farm, Wangqing Forestry Bureau, Jilin Province.

[0119] b) Construction of the trap: The trap mainly consists of a funnel-shaped bark beetle trap, purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large and small plates are crossed to form a cross shape (500mm high, 300mm wide), connected to the top cover via a hook at the top, and connected to the funnel via a hook at the bottom. The flared opening for connecting the insect collection bottle is fitted over the lower part of the funnel and connected to the collection bottle.

[0120] c) Hanging position of the repellent: Hang the drug combination in the middle position 20cm from the top of the funnel-shaped bark beetle trap. The drug combination consists of one attractant and one repellent for the spruce bark beetle (open the aluminum foil before use to get the attractant and repellent slow-release bags).

[0121] d) Field setup of the traps: Three replicates were set up for each experimental treatment. The traps were set up at the edge of the Korean pine forest, with a spacing of about 100m between the traps. Two 3m long and 0.5cm thick wires were connected between the two trees, and the traps were fixed in the middle of the wires. The insect collection bottle of the trap was 1m off the ground.

[0122] e) Data collection and trap emptying: Each treatment in this experiment was replicated three times, and the number of bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of bark beetles in the collection bottles was observed every 7 days. After counting, the collected bark beetles were placed in a plastic box and taken to the laboratory.

[0123] The application of the above-described specific steps for repelling the spruce bark beetle was carried out. From May 15th to June 10th, 2026, the bark beetle attractant (treatment 1) was used as a positive control. Specific results are shown in Table 4. Figure 4 The traps were spaced 100m apart, and the collection bottles were approximately 1m off the ground. Each treatment was replicated three times, and the number of bark beetles captured in each treatment was the average of the three traps. The number of bark beetles in the collection bottles was observed every 7 days, depending on weather conditions.

[0124] Table 4 Comparison of the repellency behavior of various repellents against the spruce bark beetle.

[0125]

[0126] From Table 4 and Figure 4 The results show that, based on the analysis of the insect-attracting effect, both the control treatment (treatment 1) and treatment 2 have good attraction activity for Bark Borer spruce. This indicates that 4-allyl anisole has no effect on the trapping amount of Bark Borer spruce and that 4-allyl anisole cannot be used as an antagonist for Bark Borer spruce attractants.

[0127] Comparative Example 2

[0128] Comparative results of experiments on the repellency behavior of 4-allyl anisole against the larch bark beetle.

[0129] The larch bark beetle attractant in this embodiment comprises bark beetle dienol and bark beetle enol. The larch bark beetle repellent is prepared using commercially available 4-allyl anisole.

[0130] The following two treatments are prepared according to the test requirements.

[0131] Treatment 1: 100 mg of barbitured terpenol and 100 mg of barbitured terpenol;

[0132] Treatment 2: 100 mg of barbitured diol, 100 mg of barbitured diol, and 4 g of 4-allyl anisole;

[0133] (1) Bark beetle diene alcohol and bark beetle alcohol were added dropwise onto a medical gauze (2g) carrier according to the above usage amount, and then a spruce bark beetle attractant was prepared. 4-Allyl anisole was added dropwise onto a medical gauze (2g) carrier according to the above usage amount, and then a sustained-release repellent was prepared.

[0134] (2) Preparation and storage of attractants and repellents: The attractant and repellent components were respectively added to a medical gauze carrier, then placed in a 16-mil black polyethylene plastic bag, and sealed with a sealing machine to obtain attractant and repellent sustained-release bags; the polyethylene plastic bag was made of low-density polyethylene with a mass percentage of 99%, a thickness of 16 mil, a length of 80 mm, and a width of 60 mm. The prepared attractant and repellent sustained-release bags were sealed with aluminum foil and refrigerated at 4℃.

[0135] (3) Field application of repellents:

[0136] a) Experiment location: Amur Forestry Bureau, Daxinganling Region, Heilongjiang Province.

[0137] b) Construction of the trap: The trap mainly consists of a funnel-shaped bark beetle trap, purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large and small plates are crossed to form a cross shape (500mm high, 300mm wide), connected to the top cover via a hook at the top, and connected to the funnel via a hook at the bottom. The flared opening for connecting the insect collection bottle is fitted over the lower part of the funnel and connected to the collection bottle.

[0138] c) Hanging position of the repellent: Hang the drug combination in the middle position 20cm from the top of the funnel-shaped bark beetle trap. The drug combination consists of one larch bark beetle attractant (purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd.) and one repellent (open the aluminum foil before use to get the attractant and repellent slow-release bag).

[0139] d) Field setup of the traps: Three replicates were set up for each experimental treatment. The traps were set up at the edge of the Korean pine forest, with a spacing of about 100m between the traps. Two 3m long and 0.5cm thick wires were connected between the two trees, and the traps were fixed in the middle of the wires. The insect collection bottle of the trap was 1m off the ground.

[0140] e) Data collection and trap emptying: Each treatment in this experiment was replicated three times, and the number of bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of bark beetles in the collection bottles was observed every 7 days. After counting, the collected bark beetles were placed in a plastic box and taken to the laboratory.

[0141] The application of the larch bark beetle attractant (treatment 1) was carried out according to the specific steps described above. From June 1st to June 21st, 2026, the larch bark beetle attractant was used as a positive control. Specific results are shown in Table 5. Figure 5 The traps were spaced 100m apart, and the collection bottles were approximately 1m off the ground. Each treatment was replicated three times, and the number of bark beetles captured in each treatment was the average of the three traps. The number of bark beetles in the collection bottles was observed every 7 days, depending on weather conditions.

[0142] Table 5. Comparison of the repellency behavior of various repellents against the larch bark beetle.

[0143]

[0144] From Table 5 and Figure 5 The results show that, based on the analysis of the insect-attracting effect, both the control treatment (treatment 1) and treatment 2 have good attraction activity against the larch bark beetle. This indicates that 4-allyl anisole has no effect on the trapping amount of the larch bark beetle, and 4-allyl anisole cannot be used as an antagonist for larch bark beetle attractants.

[0145] In summary, the bark beetle repellent provided by this invention has a significant antagonistic effect on the aggregation behavior of the bark beetle, and can effectively reduce the infection rate of the bark beetle on host plants. The bark beetle repellent provided by this invention has a simple preparation process and simple application technology, making it suitable for large-scale promotion and application in production.

[0146] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

Application of 1,4-allyl anisole in controlling damage caused by Bark beetle in forests.

2. The application according to claim 1, characterized in that, The 4-allyl anisole repels the dodecanotic bark beetle.

3. A repellent for the bark beetle, characterized in that, The repellent contains 1-8g of 4-allyl anisole.

4. The repellent according to claim 3, characterized in that, The repellent contains 4g of 4-allyl anisole.

5. A slow-release bag for repelling the dodecanotic bark beetle, characterized in that, Includes a sustained-release carrier, medical gauze placed inside the sustained-release carrier, and 4-allyl anisole adsorbed on the medical gauze; The content of the 4-allyl anisole is 1~8g.

6. The sustained-release bag according to claim 5, characterized in that, The content of the 4-allyl anisole is 4g.

7. The sustained-release bag according to claim 5, characterized in that, Each slow-release bag contains 2g of Chinese medicine gauze; The slow-release carrier is a polyethylene plastic bag; the polyethylene plastic bag is low-density polyethylene with a mass percentage of 99%, a thickness of 16 mils, a length of 80 mm, and a width of 60 mm.

8. A method for preparing the sustained-release bag according to any one of claims 5 to 7, characterized in that, Includes the following steps: The 4-allyl anisole was dropped onto medical gauze, then placed in a sustained-release carrier and sealed.

9. The use of the slow-release bag according to any one of claims 5 to 7 in controlling the damage caused by the twelve-toothed bark beetle in forests.

10. The application according to claim 9, characterized in that, The slow-release bags are fixed at a height of 1m at the base of the pine tree trunk, with one slow-release bag hanging on each affected pine tree.

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