Broad temperature range microbial corrosion inhibitor package, method of making, and methods of use thereof

Abstract

The present application relates to the technical field of bactericides, and provides a wide-temperature-range microbial corrosion compounded inhibitor, a preparation method and a use method thereof for preventing and controlling microbial corrosion under different service temperature conditions.The wide-temperature-range microbial corrosion compounded inhibitor comprises copper-silver nano composite particles and tobacco stem extract, wherein the mass content of the copper-silver nano composite particles is 10 ppm to 20 ppm, and the mass content of the tobacco stem extract is 100 ppm to 150 ppm.The wide-temperature-range microbial corrosion compounded inhibitor can inhibit the microbial corrosion process induced by normal-temperature sulfate-reducing bacteria and high-temperature thermophilic sulfate-reducing bacteria, and can realize the synergistic control of the attached bacteria on the surface of a metal substrate and free corrosion bacteria in water, so as to reduce the local corrosion and persistent corrosion damage caused by microbial metabolites.The present application can construct a wide-temperature-range microbial corrosion inhibition system suitable for normal-temperature and high-temperature environments for preventing and controlling microbial corrosion under different service temperature conditions.

Description

Technical Field

[0001] This invention belongs to the field of bactericide technology, specifically relating to a wide-temperature-range microbial corrosion compound inhibitor, its preparation method, and its application method. Background Technology

[0002] Microbiologically influenced corrosion (MIC) refers to the phenomenon where microorganisms and their metabolic activities cause or accelerate the corrosion of metallic materials by altering the metal / solution interface, promoting electrochemical reactions, and forming corrosive metabolic products. This corrosion process is widespread in industrial environments such as oil and gas extraction and transportation, shale gas development, marine engineering, chemical storage and transportation, underground pipelines, and geothermal energy utilization. Microbiological corrosion not only leads to localized corrosion, pitting, and crack propagation on the surface of metallic materials, but can also cause safety accidents such as pipeline perforation and equipment leaks, thereby affecting the service life and operational safety of industrial equipment.

[0003] Currently, adding chemical disinfectants to service systems is one of the most common methods for controlling microbial corrosion. Traditional disinfectants mainly include aldehydes, quaternary ammonium salts, isothiazolinones, and organophosphates, which primarily achieve their bactericidal effect by disrupting the cell membrane structure of microorganisms, inhibiting the activity of key metabolic enzymes, or interfering with intracellular metabolic processes. However, with the long-term and frequent use of disinfectants in industrial systems, microorganisms have gradually developed adaptability and tolerance, leading to problems such as increased effective concentrations, decreased bactericidal efficiency, and increased risk of secondary pollution in practical applications.

[0004] Furthermore, microbial corrosion in industrial environments is typically not caused by microorganisms operating under single-temperature conditions. In ambient oil and gas extraction environments, sulfate-reducing bacteria (SRBs) can produce sulfides through sulfate reduction, promoting the depolarization process of metal cathodes and accelerating carbon steel corrosion. In deep oil and gas resource development, geothermal environments, and high-temperature industrial systems, some thermophilic bacteria and thermophilic archaea can maintain metabolic activity and induce severe corrosion of metallic materials. Therefore, practical industrial environments demand bactericides with wide temperature range adaptability.

[0005] Most existing chemical bactericides are developed for low- and medium-temperature environments, and their bactericidal activity is easily affected by factors such as temperature increases, changes in media composition, and the formation of microbial biofilms. Under high-temperature conditions, some organic bactericides are prone to thermal degradation or loss of active components, resulting in reduced bactericidal ability. At the same time, the extracellular polymeric substances (EPS) formed by microorganisms on metal surfaces can constitute a physical diffusion barrier, restricting the transfer of bactericide molecules into the biofilm, making it difficult for traditional bactericides to effectively remove attached microorganisms. This leads to the continuous proliferation of residual microorganisms and induces long-term corrosion of metal materials.

[0006] Therefore, in view of the problems of increased drug resistance, failure in high-temperature environment, obstruction by biofilm barrier and limited temperature adaptability of existing microbial corrosion bactericides, there is an urgent need to develop a new type of microbial corrosion control material with wide temperature range adaptability, capable of inhibiting both room temperature corrosive bacteria and high temperature corrosive microorganisms, and possessing high efficiency, long-lasting effect and green environmental protection characteristics. Summary of the Invention

[0007] To address the shortcomings of the existing technologies, this invention provides a wide-temperature-range microbial corrosion compound inhibitor, its preparation method, and its application method. The wide-temperature-range microbial corrosion compound inhibitor can remove bacteria attached to the surface of metal substrates in medium- and high-temperature oil and gas field environments, and can also kill free corrosion bacteria in water, thereby avoiding the continuous corrosion of metal substrates by the large accumulation of bacteria after peeling.

[0008] The first aspect of this invention provides a wide-temperature-range microbial corrosion inhibitor, comprising copper-silver nanocomposite particles and tobacco stem extract; wherein the mass content of the copper-silver nanocomposite particles is 10ppm to 20ppm; and the mass content of the tobacco stem extract is 100ppm to 150ppm.

[0009] Optionally, the copper-silver nanocomposite particles have a particle size of 10 nm to 20 nm, wherein the copper-silver nanocomposite particles are mixed particles with particle sizes of 10 nm and 20 nm. Optionally, the tobacco stem extract is an organic active ingredient extracted from tobacco stems.

[0010] Optionally, the copper-silver nanocomposite particles have a mass content of 10 ppm; the tobacco stem extract has a mass content of 100 ppm.

[0011] A second aspect of the present invention provides a method for preparing the above-mentioned wide-temperature-range microbial corrosion compound inhibitor, comprising: Step 1: Dissolve the aqueous copper-silver nanocomposite particles in sterile water to obtain a copper-silver nanocomposite particle stock solution. Step 2: Dissolve the tobacco stem extract powder in sterile water or a weakly alkaline aqueous solution to obtain a tobacco stem extract stock solution; Step 3: Mix the copper-silver nanocomposite particle stock solution and the tobacco stem extract stock solution to obtain the wide-temperature-range microbial corrosion compound inhibitor; In step 3, the mass content of copper-silver nanocomposite particles in the wide-temperature-range microbial corrosion compound inhibitor is 10ppm~20ppm, and the mass content of tobacco stem extract is 100ppm~150ppm.

[0012] A third aspect of the present invention provides a method for using the above-mentioned wide-temperature-range microbial corrosion compound inhibitor, comprising: bringing the surface of a substrate into contact with the microbial corrosion compound inhibitor.

[0013] Optionally, the substrate is a pipe.

[0014] Alternatively, the pipeline may be made of X60 pipeline steel.

[0015] The fourth aspect of the present invention provides an application of the above-mentioned wide-temperature-range microbial corrosion compound inhibitor, which is applied in a wide temperature range of 30℃ to 70℃.

[0016] Optionally, it can be used to inhibit microbial corrosion of the substrate surface caused by ambient-temperature sulfate-reducing bacteria and thermophilic sulfate archaea.

[0017] This invention provides a wide-temperature-range microbial corrosion compound inhibitor, its preparation method, its usage method, and its application. It uses copper-silver nanocomposite particles as an effective bactericidal component to kill microorganisms in corrosive environments; and tobacco stalk extract as a biofilm-dispersing component to disrupt the microbial biofilm structure on the metal substrate surface, promote the detachment of attached bacteria from the metal surface, and inhibit bacterial re-attachment to form a biofilm. The copper-silver nanocomposite particles are inorganic nanomaterials, and their antibacterial effect mainly originates from the release of metal ions and nanoscale effects, making them insensitive to changes in environmental temperature. The plant-derived active ingredients in the tobacco stalk extract can maintain certain biological activity under different temperature conditions. Therefore, the combination of these two components can achieve stable antibacterial performance over a wide temperature range. By selecting a combination of copper-silver nanocomposite particles and tobacco stem extract as a wide-temperature-range microbial corrosion inhibitor, the synergistic effect of the two can be achieved. This wide-temperature-range microbial corrosion inhibitor can not only remove bacteria attached to the surface of the metal substrate, but also kill free corrosion bacteria in the water. This avoids the large accumulation of bacteria after peeling and continuous corrosion of the metal substrate. It can also inhibit both room-temperature and high-temperature corrosion bacteria at the same time. It is a new type of microbial corrosion control material with high efficiency, long-lasting effect and green environmental protection characteristics.

[0018] Other features and advantages of the invention will be set forth in the following description, and will be apparent in part from the description, or may be learned by practicing the invention. The objects and other advantages of the invention may be realized and obtained by means of the structures particularly pointed out in the written description and the accompanying drawings.

[0019] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0020] Figure 1 The images show the biofilm morphology of sulfate-reducing bacteria after 7 days of culture, obtained from the blank control group 1 and application examples 1 to 3, and the images obtained from laser confocal microscopy with live and dead staining. ((a) is blank control group 1; (b) is application example 1; (c) is application example 2; (d) is application example 3.) Figure 2 The images show the maximum pitting morphology and depth statistics of X60 carbon steel samples after 7 days of treatment with sulfate-reducing bacteria at room temperature, for the blank control group 1 and application examples 1 to 3 respectively; (a) Blank control group 1; (b) Application example 1; (c) Application example 2; (d) Application example 3; (e) Corrosion rate diagram; (f) Pits distribution diagram. Figure 3 Electrochemical test results of X60 carbon steel samples treated with sulfate-reducing bacteria at room temperature after blank control group 1 and application examples 1 to 3 respectively: (a) Open circuit potential (OCP) change curve; (b) Charge transfer resistance (…). R ct (c) is the change curve; (d) is the potentiodynamic polarization curve; (c) is the corrosion current density curve. i corr Statistical results); Figure 4 To count the number of free sulfate-reducing bacteria in the culture medium at room temperature and the number of bacteria attached to the surface of X60 pipeline steel samples after treatment with blank control group 1 and application examples 1 to 3, respectively, using the hemocytometer method; Figure 5 The images show the morphology of the thermophilic sulfate archaeal biofilms after 7 days of culture, as shown by scanning electron microscopy, laser confocal microscopy images of live and dead organisms after treatment with blank control group 2 and application examples 4 to 6 respectively; (a) blank control group 2; (b) application example 4; (c) application example 5; (d) application example 6. Figure 6The images show the maximum pitting morphology and depth statistics of X60 carbon steel samples after 7 days of treatment with thermophilic sulfate archaea, specifically for the blank control group 2 and application examples 4 to 6. ((a) is blank control group 2; (b) is application example 4; (c) is application example 5; (d) is application example 6; (e) is corrosion rate diagram; (f) is pit distribution diagram). Figure 7 Electrochemical test results of X60 carbon steel samples after treatment with thermophilic sulfate archaea in blank control group 2 and application examples 4 to 6, respectively, are shown. (a) is the open circuit potential (OCP) curve; (b) is the charge transfer resistance curve. R ct (c) is the change curve; (d) is the potentiodynamic polarization curve; (e) is the corrosion current density curve. i corr Statistical results); Figure 8 To count the number of free thermophilic sulfate archaea in the culture medium and the number of attached bacteria on the surface of X60 pipeline steel samples after treatment with blank control group 2 and application examples 4 to 6, the hemocytometer method was used. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below in conjunction with the embodiments of this invention. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0022] The first aspect of this invention provides a wide-temperature-range microbial corrosion compound inhibitor, comprising copper-silver nanocomposite particles and tobacco stem extract; wherein the mass content of the copper-silver nanocomposite particles is 10ppm~20ppm and the mass content of the tobacco stem extract is 100ppm~150ppm.

[0023] The wide-temperature-range microbial corrosion compound inhibitor provided in this application uses copper-silver nanocomposite particles as the basic bactericidal core of the microbial corrosion compound inhibitor system. Specifically, the copper-silver nanocomposite particles directly kill free corrosive microorganisms in the corrosive environment. The lower limit of the copper-silver nanocomposite particle mass content is set at 10 ppm to ensure the basic bactericidal ability of the microbial corrosion compound inhibitor, while the upper limit is set at 20 ppm to avoid excessive use of copper-silver nanocomposite particles, which would increase on-site costs and cause pesticide residues. The mass content of the copper-silver nanocomposite particles can be one of 10 ppm, 15 ppm, and 20 ppm, but is not limited to these values.

[0024] Copper-silver nanocomposite particles can serve as the core bactericidal component in microbial corrosion inhibitor formulations, possessing the synergistic antibacterial properties of both copper and silver. Due to their small nanoscale size and large specific surface area, these particles can effectively penetrate the extracellular polymer matrix barrier formed by microorganisms and achieve uniform dispersion on metal substrates and in aqueous environments. Through the continuous release of Cu... 2+ and Ag + Copper-silver nanocomposite particles can disrupt the cell membrane structure of microorganisms, promote intracellular protein inactivation and nucleic acid damage, and induce the generation of reactive oxygen species, triggering oxidative stress responses and inhibiting microbial metabolic activities at multiple sites. Therefore, a mass content of 10 ppm for copper-silver nanocomposite particles can be used as the minimum effective auxiliary bactericidal dosage, while a mass content of 20 ppm can control costs and the risk of silver ion enrichment. The mass content of copper-silver nanocomposite particles can be one of 10 ppm, 15 ppm, or 20 ppm, but is not limited to these values.

[0025] Tobacco stalk extract can serve as a plant-derived functional enhancer in compound inhibitors of microbial corrosion. Because it contains active ingredients, it can participate in the regulation of microbial corrosion processes through multiple mechanisms. On one hand, the active molecules in tobacco stalk extract can adsorb onto the surface of microbial cell membranes, inhibiting corrosion microorganisms by altering cell membrane permeability, affecting intracellular metabolic processes, and suppressing the activity of key enzymes. On the other hand, its abundant polar functional groups can interact with copper-silver nanocomposite particles, improving the dispersion stability of nanoparticles in corrosive media, reducing aggregation, and promoting the uniform distribution of active components on the metal matrix surface and in areas rich in microorganisms. Since tobacco-based bactericides have limited ability to kill microorganisms, a mass content of 100 ppm for tobacco stalk extract is selected as the minimum effective auxiliary bactericidal dosage. The mass content of tobacco stalk extract can be one of 100 ppm, 125 ppm, or 150 ppm, but is not limited to these values.

[0026] Furthermore, since copper-silver nanocomposite particles are inorganic nanomaterials, their antibacterial effect mainly originates from the release of metal ions and nanoscale effects, and they are insensitive to changes in environmental temperature. The plant-derived active ingredients in tobacco stem extract can maintain certain biological activity under different temperature conditions. Therefore, the combination of the two can achieve stable antibacterial performance over a wide temperature range. Thus, by setting a wide-temperature-range microbial corrosion inhibitor as a composite inhibition system composed of copper-silver nanocomposite particles and tobacco stem extract, the synergistic effect between different functional components can be fully utilized. Specifically, the plant-derived active ingredients in the tobacco stem extract can first act on the extracellular polymer matrix of microorganisms, disrupting the structural stability of the biofilm, reducing microbial adhesion, and promoting biofilm loosening, thereby exposing protected immobilized corrosive microorganisms to the external environment and improving the efficiency of subsequent bactericidal component delivery to the biofilm. Further, copper-silver nanocomposite particles can rapidly inactivate free corrosive microorganisms in the liquid phase, reducing the number of corrosive bacteria and inhibiting microbial metabolic activity. In addition, copper-silver nanocomposite particles can enter the microbial enrichment area through nanoscale effects and continuously release Cu. + and Ag + It disrupts the integrity of microbial cell membranes, induces the generation of reactive oxygen species, causes oxidative stress damage, and inhibits the reattachment of microorganisms and the formation of new biofilms, thereby further enhancing the liquid-phase bactericidal effect and long-term antibacterial ability.

[0027] Meanwhile, this microbial corrosion compound inhibitor has good temperature adaptability and can be used to control the microbial-induced corrosion process under different temperature environments. It can simultaneously inhibit the growth and metabolic activities of room-temperature sulfate-reducing bacteria and high-temperature thermophilic sulfate-corroding microorganisms, thereby reducing the uniform corrosion rate and local corrosion degree of the metal matrix, delaying the microbial-induced corrosion process, and improving the service safety of metal materials in complex industrial environments.

[0028] In this embodiment, copper-silver nanocomposite particles can be used as the basic bactericidal component to kill microorganisms in a corrosive environment; tobacco stem extract can be used as the biofilm dispersing component to disrupt the microbial biofilm structure on the metal substrate surface and promote the detachment of attached bacteria from the metal surface; and inhibit bacterial reattachment to form a biofilm. Therefore, by selecting a combination of copper-silver nanocomposite particles and tobacco stem extract as the wide-temperature-range microbial corrosion compound inhibitor, the synergistic effect between the two can be achieved. This wide-temperature-range microbial corrosion compound inhibitor can both remove bacteria attached to the metal substrate surface and kill free corrosion bacteria in water in medium- and high-temperature oil and gas field environments, thereby avoiding the large-scale accumulation of bacteria after peeling and continuous corrosion of the metal substrate, and can also inhibit both room-temperature and high-temperature corrosion bacteria at the same time.

[0029] In some possible embodiments, the particle size of the copper-silver nanocomposite particles is 10 nm to 20 nm.

[0030] Here, the particle size of the copper-silver nanocomposite particles is one of 10nm, 15nm, or 20nm, but is not limited to these. By limiting the particle size of the copper-silver nanocomposite particles to 10~20nm, the selected copper-silver nanocomposite particles can utilize their nanoscale effect and the intrinsic broad-spectrum antibacterial activity of silver and copper elements to assist microbial corrosion inhibitors in sterilization.

[0031] Among them, the copper-silver nanocomposite particles are a mixture of particles with a particle size of 10nm and 20nm, and the mixing ratio of particles with a particle size of 10nm and 20nm can be 1:0.8~2.

[0032] In some possible embodiments, the tobacco stem extract is an organic active ingredient extracted from tobacco stems.

[0033] Here, the organic active ingredient can be one or more of nicotine, polyphenolic compounds, flavonoids, and various active ingredients with oxygen-containing functional groups.

[0034] In this embodiment, tobacco stem extract is selected as an organic active ingredient extracted from tobacco stems, which can effectively regulate the microbial corrosion process through multiple mechanisms of action.

[0035] In some possible embodiments, the mass content of the copper-silver nanocomposite particles is 10 ppm; the mass content of the tobacco stem extract is 100 ppm.

[0036] Here, when the mass content of copper-silver nanocomposite particles is selected as 10 ppm and the mass content of tobacco stem extract is selected as 100 ppm, the synergistic effect between copper-silver nanocomposite particles and tobacco stem extract is optimal, enabling the wide-temperature-range microbial corrosion compound inhibitor provided by this invention to remove bacteria attached to the surface of the metal substrate.

[0037] A second aspect of the present invention provides a method for preparing the above-mentioned wide-temperature-range microbial corrosion compound inhibitor, comprising: Step 1: Dissolve the aqueous copper-silver nanocomposite particles in sterile water to obtain a copper-silver nanocomposite particle stock solution. Step 2: Dissolve the tobacco stem extract powder in sterile water or a weakly alkaline aqueous solution to obtain a tobacco stem extract stock solution; Step 3: Mix the copper-silver nanocomposite particle stock solution and the tobacco stem extract stock solution to obtain the wide-temperature-range microbial corrosion compound inhibitor; In step 3, the mass content of copper-silver nanocomposite particles in the wide-temperature-range microbial corrosion compound inhibitor is 10ppm~20ppm, and the mass content of tobacco stem extract is 100ppm~150ppm.

[0038] Here, the copper-silver nanocomposite particles can be synthesized by different methods, and the particle size can be 1 nm to 100 nm; the mass content of the copper-silver nanocomposite particles can be 10 ppm to 20 ppm; and the mass content of the tobacco stem extract can be 100 ppm to 150 ppm. Preferably, the mass content of the copper-silver nanocomposite particles is 10 ppm, and the mass content of the tobacco stem extract is 100 ppm.

[0039] The method for preparing a wide-temperature-range microbial corrosion compound inhibitor provided by this invention involves mixing a stock solution containing copper-silver nanocomposite particles and a stock solution containing tobacco stem extract, with the final microbial corrosion compound inhibitor containing 10ppm~20ppm of copper-silver nanocomposite particles and 100ppm~150ppm of tobacco stem extract by mass. Therefore, the method for preparing the microbial corrosion compound inhibitor provided by this application does not require complex synthesis steps or harsh reaction conditions; the final wide-temperature-range microbial corrosion compound inhibitor can be obtained simply by mixing.

[0040] A third aspect of the present invention provides a method for using the above-mentioned wide-temperature-range microbial corrosion compound inhibitor, comprising: bringing the surface of a substrate into contact with the wide-temperature-range microbial corrosion compound inhibitor.

[0041] When the wide-temperature-range microbial corrosion compound inhibitor provided by this invention is present on the surface of the substrate, the biofilm on the substrate surface is significantly destroyed by the microbial corrosion compound inhibitor, the number of attached and free bacteria is significantly reduced, the corrosion rate and pitting depth are significantly reduced, and the composite treatment system formed by the microbial corrosion compound inhibitor has an excellent inhibitory effect on microbial corrosion induced by corrosive microorganisms at different temperatures.

[0042] In some possible implementations, the substrate is a pipe.

[0043] Here, the pipeline can be an oil or gas pipeline.

[0044] In some possible implementations, the pipeline is made of X60 pipeline steel.

[0045] Here, the main components of X60 pipeline steel by mass percentage are: 0.14 C, 0.10 Si, 1.31 Mn, 0.12 Mo, 0.02 Ni, 0.03 Cu, 0.30 Cr, 0.03 V, 0.01 P, 0.01 S, with Fe as the balance.

[0046] In this embodiment, by selecting X60 pipeline steel, which is commonly used in oil and gas pipelines, it can target typical corrosive microorganisms in two different temperature ranges: room temperature sulfate-reducing bacteria and room temperature sulfate-reducing bacteria. This makes it suitable for shale gas flowback fluid treatment systems, oil and gas gathering and transportation pipelines, and other environments where microbial corrosion occurs.

[0047] The fourth aspect of the present invention provides an application of the above-mentioned wide-temperature-range microbial corrosion compound inhibitor, wherein the wide-temperature-range microbial corrosion compound inhibitor is applied in a wide temperature range of 30℃ to 70℃.

[0048] Preferably, it is used to inhibit microbial corrosion of the substrate surface caused by sulfate-reducing bacteria and thermophilic sulfate archaea.

[0049] The wide-temperature-range microbial corrosion compound inhibitor provided by this invention has a significant inhibitory effect on microbial corrosion, especially in the system of sulfate-reducing bacteria at room temperature (30°C), where the corrosion inhibition rate reaches 90% through the combined treatment of copper-silver nanocomposite particles and tobacco stem extract in the wide-temperature-range microbial corrosion compound inhibitor; in the system of thermophilic sulfate archaea at high temperature (70°C), the corrosion inhibition rate reaches 87% through the combined treatment of copper-silver nanocomposite particles and tobacco stem extract in the wide-temperature-range microbial corrosion compound inhibitor, and it significantly reduces the maximum pitting depth on the metal surface. Therefore, the microbial corrosion compound inhibitor provided by this invention can exhibit consistent biofilm destruction and corrosion inhibition patterns against corrosive bacteria at different temperature ranges. Thus, this wide-temperature-range microbial corrosion compound inhibitor can be used to inhibit microbial corrosion caused by sulfate-reducing bacteria and thermophilic sulfate archaea in different temperature scenarios.

[0050] The following examples illustrate the wide-temperature-range microbial corrosion inhibitor, its preparation method, its usage, and its applications provided by this invention. It should be noted that, unless otherwise specified, the methods, reagents, and materials described in the following examples are all commercially available; and the experimental methods described are conventional methods unless otherwise specified.

[0051] Example 1 A method for preparing a wide-temperature-range microbial corrosion compound inhibitor includes: Step 1: Add aqueous copper-silver nanocomposite particles A to sterile water. The particle size of aqueous copper-silver nanocomposite particles A is about 20 nm. Through ultrasonic dispersion treatment, the copper-silver nanocomposite particles A are uniformly dispersed in the solution system to obtain a dispersion of copper-silver nanocomposite particles A. Step 2: Add the tobacco stem extract powder to sterile water and shake thoroughly to mix, so that the plant-derived active ingredients in the tobacco stem extract are fully dissolved and evenly dispersed, to obtain the tobacco stem extract stock solution; Step 3: According to the target final concentration, mix the tobacco stem extract stock solution with the copper-silver nanocomposite particle A dispersion solution, and stir thoroughly to obtain a wide-temperature-range microbial corrosion compound inhibitor. The copper-silver nanocomposite particle A content in the microbial corrosion compound inhibitor is 10 ppm, and the tobacco stem extract content is 100 ppm. After thorough mixing, the treatment system of the compound microbial corrosion inhibitor is obtained.

[0052] Example 2 A method for preparing a wide-temperature-range microbial corrosion compound inhibitor includes: Step 1: Based on copper-silver nanocomposite particles A, the aqueous sol containing copper-silver nanocomposite particles A is first ultrasonically dispersed to reduce particle agglomeration and improve the dispersion uniformity of the system. Then, size-selective differential centrifugation is used to remove larger particles and residual agglomerates, and the component enriched with small-diameter nanoparticles is collected. The obtained component is redispersed to obtain aqueous copper-silver nanocomposite particles B, with an average particle size of approximately 10 nm. Step 2: Add aqueous copper-silver nanocomposite particles B to sterile water. The particle size of aqueous copper-silver nanocomposite particles B is about 10 nm. Perform ultrasonic dispersion treatment to make copper-silver nanocomposite particles B uniformly distributed in the solution system to obtain copper-silver nanocomposite particle B dispersion. Step 3: Add the tobacco stem extract powder to sterile water, and treat it by shaking and stirring to fully dissolve the active substances in the tobacco stem extract to obtain tobacco stem extract stock solution; Step 4: According to the target final concentration, mix the tobacco stem extract stock solution and the copper-silver nanocomposite particle B dispersion to obtain a wide-temperature-range microbial corrosion compound inhibitor. The copper-silver nanocomposite particle B content in the microbial corrosion compound inhibitor is 10 ppm, and the tobacco stem extract content is 100 ppm. After thorough mixing, the treatment system of the compound microbial corrosion inhibitor is obtained.

[0053] Example 3 A method for preparing a wide-temperature-range microbial corrosion compound inhibitor includes: Step 1: Add copper-silver nanocomposite particles A to sterile water and perform ultrasonic dispersion treatment to make copper-silver nanocomposite particles A uniformly distributed in the solution system, thus obtaining a dispersion of copper-silver nanocomposite particles A. Step 2: Add tobacco stem extract powder to sterile water, and treat by shaking and stirring to fully dissolve the active substances in tobacco stem extract to obtain tobacco stem extract stock solution; Step 3: According to the target final concentration, mix the tobacco stem extract stock solution and the copper-silver nanocomposite particle A dispersion to obtain a wide-temperature-range microbial corrosion compound inhibitor. The copper-silver nanocomposite particle A content in the microbial corrosion compound inhibitor is 20 ppm, and the tobacco stem extract content is 150 ppm. After thorough mixing, the treatment system of the compound microbial corrosion inhibitor is obtained.

[0054] Comparative Example 1 A method for preparing a biocorrosion compound inhibitor, comprising: Step 1: Add tobacco stem extract to sterile water and shake thoroughly to mix, so that the plant-derived active ingredients in the tobacco stem extract are evenly dispersed, and obtain tobacco stem extract stock solution; Step 2: Add the tobacco stem extract stock solution to the corrosive microbial culture system according to the target final concentration, so that the final concentration of tobacco stem extract in the system is 100 ppm. After mixing evenly, the tobacco stem extract treatment system is obtained.

[0055] Blank control group 1 A method for preparing a corrosive microbial culture system, comprising the following steps: 1. The X60 pipeline steel sample was successively polished with SiC sandpaper with grit sizes of P150, P240, P400, P600, P800 and 1000. Then it was ultrasonically cleaned in distilled water and anhydrous ethanol respectively, dried and sterilized with ultraviolet light for later use. 2. The treated X60 pipeline steel samples were placed in a simulated shale gas field environment culture medium inoculated with room-temperature sulfate-reducing bacteria (the main components of which include sodium chloride, sodium sulfate, sodium bicarbonate, potassium chloride, potassium bromide, boric acid, magnesium chloride, strontium chloride, calcium chloride, sodium L-lactic acid, and yeast extract; the culture medium was autoclaved at 121℃ for 20 min, then deoxygenated by a mixture of carbon dioxide (20%) and nitrogen (80%) gas, and used under anaerobic conditions). The samples were then cultured anaerobicly at 30℃ for 7 days. After the culture period, the X60 pipeline steel samples were removed, stained using the LIVE / DEAD live / dead bacteria staining kit, and the activity and thickness of the surface biofilm on the X60 pipeline steel samples were observed using a laser confocal microscope to obtain the corrosive microbial culture system.

[0056] Application Example 1 (100 ppm of tobacco stem extract) Add the tobacco extract stock solution from Comparative Example 1 to the corrosive microbial culture system obtained from the blank control group 1 according to the target final concentration, so that the final concentration of tobacco extract in the corrosive microbial culture system is 100 ppm. After mixing evenly, the tobacco extract in the system can be brought into contact with the surface biofilm of the X60 pipeline steel sample.

[0057] Application Example 2 (100 ppm tobacco stem extract + 10 ppm copper-silver nanocomposite particles A) The wide-temperature-range microbial corrosion compound inhibitor from Example 1 was added to the corrosive microbial culture system obtained from the blank control group 1 according to the target final concentration, so that the final concentration of copper-silver nanocomposite particles A in the system was 10 ppm and the mass content of tobacco stem extract was 100 ppm. After mixing evenly, the compound system of copper-silver nanocomposite particles A and tobacco stem extract in the system can be brought into contact with the surface biofilm of X60 pipeline steel sample.

[0058] Application Example 3 (100 ppm tobacco stem extract + 10 ppm copper-silver nanocomposite particles B) The wide-temperature-range microbial corrosion compound inhibitor from Example 2 was added to the corrosive microbial culture system obtained from the blank control group 1 according to the target final concentration, so that the final concentration of copper-silver nanocomposite particles B in the system was 10 ppm and the mass content of tobacco stem extract was 100 ppm. After mixing evenly, the compound system of copper-silver nanocomposite particles B and tobacco stem extract in the system can be brought into contact with the surface biofilm of X60 pipeline steel sample.

[0059] Blank control group 2 A method for preparing a corrosive microbial culture system, comprising the following steps: 1. The X60 pipeline steel sample was successively polished with SiC sandpaper with grit sizes of P150, P240, P400, P600, P800 and P1000. Then it was ultrasonically cleaned in distilled water and anhydrous ethanol respectively, dried and sterilized with ultraviolet light for later use. 2. The treated X60 pipeline steel sample was placed in a simulated shale gas field environment culture medium inoculated with thermophilic sulfate archaea (the main components of which include sodium chloride, sodium sulfate, sodium bicarbonate, potassium chloride, potassium bromide, boric acid, magnesium chloride, strontium chloride, calcium chloride, sodium L-lactic acid and yeast extract. The culture medium was sterilized at 121℃ for 20 min, and then deoxygenated by a mixture of carbon dioxide (20%) and nitrogen (80%) gas and used under anaerobic conditions). The sample was cultured at 30℃ for 7 days under anaerobic conditions.

[0060] Application Example 4 (100 ppm of tobacco stem extract) Add the tobacco extract stock solution from Comparative Example 1 to the corrosive microbial culture system obtained from the blank control group 2 according to the target final concentration, so that the final concentration of tobacco extract in the system is 100 ppm. After mixing evenly, the tobacco extract in the system can be brought into contact with the biofilm on the surface of the X60 pipeline steel sample.

[0061] Application Example 5 (100 ppm tobacco stem extract + 10 ppm copper-silver nanocomposite particles A) The wide-temperature-range microbial corrosion compound inhibitor from Example 1 was added to the corrosive microbial culture system obtained from the blank control group 2 according to the target final concentration, so that the final concentration of copper-silver nanocomposite particles A in the system was 10 ppm and the mass content of tobacco stem extract was 100 ppm. After mixing evenly, the compound system of copper-silver nanocomposite particles A and tobacco stem extract in the system can be brought into contact with the surface biofilm of X60 pipeline steel sample.

[0062] Application Example 6 (100 ppm tobacco stem extract + 10 ppm copper-silver nanocomposite particles B) The wide-temperature-range microbial corrosion compound inhibitor from Example 2 was added to the corrosive microbial culture system obtained from the blank control group 2 according to the target final concentration, so that the final concentration of copper-silver nanocomposite particles B in the system was 10 ppm and the mass content of tobacco extract was 100 ppm. After mixing evenly, the compound system of copper-silver nanocomposite particles B and tobacco extract in the system could come into contact with the surface biofilm of the X60 pipeline steel sample.

[0063] The blank control group 1 and the X60 pipeline steel samples from Application Examples 1 to 3, which were co-cultured with sulfate-reducing bacteria at room temperature for 7 days, were taken out and stained with a LIVE / DEAD live / dead bacteria staining kit. The activity and thickness of the biofilm on the surface of the X60 pipeline steel samples were observed using a laser confocal microscope. The biofilms from the blank control group 1 and the X60 pipeline steel samples from Application Examples 1 to 3, which were co-cultured with sulfate-reducing bacteria at room temperature for 7 days, were fixed with 4% glutaraldehyde for 4 hours, then dehydrated with graded ethanol, and then sputter-coated with gold to ensure the conductivity of the biofilm. The morphology of the biofilm was observed using a scanning electron microscope to obtain the corrosive microbial culture system. The entire biofilm is as follows: Figure 1 As shown, the analysis of the effects of the inhibitors corresponding to the blank control group 1 and Application Examples 1 to 3 on the biofilm activity of sulfate-reducing bacteria at room temperature confirms that: the X60 pipeline steel sample in the blank control group was covered with a dense and continuous biofilm, with bacteria extensively attached to corrosion products and extracellular polymer structures. In the X60 pipeline steel sample treated with Application Example 1, the biofilm showed discontinuities, with some substrate exposed. In the X60 pipeline steel sample treated with Application Example 2, the biofilm structure tended to disintegrate, and the bacterial coverage decreased significantly. In the X60 pipeline steel sample treated with Application Example 3, almost no intact bacteria or continuous extracellular polymer structures were observed on the sample surface.

[0064] The blank control group 1 and the X60 pipeline steel samples treated in Examples 1 to 3 were placed in the respective treatment systems and incubated for 7 days. After incubation, Clarke solution was used to remove corrosion products and biofilm from the surface of the X60 pipeline steel samples. After cleaning and drying, the sample mass was weighed, and the corrosion rate was calculated using the following formula: ;in, V C Indicates corrosion rate (mm y) 1 ), W 0 represents the sample weight (g) before soaking. W 1 represents the weight (g) of the sample after soaking. t The soaking time (h) is the soaking time. ρ The density of Q235 carbon steel (g / cm³) 3 ), A The surface area of ​​the sample exposed (cm²) 2 ).

[0065] See Figure 2 The results showed that the corrosion rate of the blank control group 1 was 0.139 mm y 1 The corrosion rate of the X60 pipeline steel sample treated in Example 1 decreased to 0.060 mm y. 1 The corrosion rate of the X60 pipeline steel sample treated in Example 2 decreased to 0.033 mm y. 1 The X60 pipeline steel sample treated with Example 3 exhibited the lowest corrosion rate, at 0.013 mmy. 1 This indicates that the combination system of tobacco stem extract and copper-silver nanocomposite particles has a significant synergistic effect in inhibiting the corrosion of sulfate-reducing bacteria at room temperature.

[0066] The X60 pipeline steel samples treated in blank control group 1 and application examples 1 to 3 were subjected to surface corrosion products and biofilm removal. The pitting morphology of the X60 pipeline steel surface was observed using a laser confocal microscope, and the maximum pitting depth was measured. Figure 2 The effects of the inhibitors corresponding to Examples 1 to 3 on pitting corrosion induced by room-temperature sulfate-reducing bacteria were analyzed, along with the blank control group. It was determined that the maximum pitting depth of the X60 pipeline steel sample in the blank control group was 13.6 μm. The maximum pitting depth of the X60 pipeline steel sample treated with Example 1 decreased to 8.5 μm; the maximum pitting depth of the X60 pipeline steel sample treated with Example 2 decreased to 4.3 μm; and the maximum pitting depth of the X60 pipeline steel sample treated with Example 3 further decreased to 3.8 μm. This demonstrates that the wide-temperature-range microbial corrosion compound inhibitor provided by this invention can significantly inhibit localized pitting corrosion caused by room-temperature sulfate-reducing bacteria.

[0067] The blank control group 1 and the X60 pipeline steel samples treated in Examples 1 to 3 were prepared as working electrodes, with a saturated calomel electrode as the reference electrode and a platinum electrode as the counter electrode, constructing a three-electrode system. Electrochemical tests were conducted under anaerobic conditions for 7 days, including open-circuit potential, linear polarization resistance, electrochemical impedance spectroscopy, and potentiodynamic polarization measurements. Figure 3 The influence of the inhibitors corresponding to test cases 1 to 3 on the electrochemical corrosion behavior of sulfate-reducing bacteria at room temperature was analyzed, and it was determined that the corrosion current density of the X60 pipeline steel sample corresponding to the blank control group was 32.1 μA cm⁻¹. 2 The corrosion current density of the X60 pipeline steel sample treated with Example 1 decreased to 22.4 μA cm⁻¹. 2 The corrosion inhibition rate was 30.2%; after treatment with Example 2, the corrosion current density of the X60 pipeline steel sample decreased to 5.4 μA cm⁻¹. 2 The corrosion inhibition rate was 83.2%; after treatment with Example 3, the corrosion current density of the X60 pipeline steel sample decreased to 1.5 μA cm⁻¹. 2 The corrosion inhibition rate was 95.3%. Electrochemical impedance spectroscopy results showed that the X60 pipeline steel sample treated with Example 3 had the highest charge transfer resistance, indicating that the electrochemical corrosion reaction was significantly inhibited.

[0068] The number of free bacteria in the culture medium and the number of bacteria attached to the surface of the X60 pipeline steel samples treated with Examples 1 to 3 were counted using the hemocytometer method. Figure 4 In addition to the blank control group, the effects of the inhibitors corresponding to Examples 1 to 3 on free and attached sulfate-reducing bacteria at room temperature were analyzed. It can be determined that the number of free and attached bacteria in the X60 pipeline steel samples treated with Examples 2 and 3 was significantly lower than that in the X60 pipeline steel samples treated with Example 1.

[0069] like Figure 5As shown, in conjunction with the blank control group 2, the effects of the inhibitors corresponding to Examples 4 to 6 on the biofilm activity of thermophilic sulfate archaea were analyzed. It can be determined that in the blank control group 2, thermophilic sulfate archaea formed a continuous biofilm mainly composed of live bacteria on the surface of the X60 pipeline steel sample. In the X60 pipeline steel sample treated with Example 4, the number of live bacteria decreased slightly, but the biofilm still maintained a certain degree of continuity. In the X60 pipeline steel sample treated with Example 5, the number of live bacteria further decreased, the biofilm structure was significantly damaged, and the bacteria were distributed in discrete dots or small clusters. In the X60 pipeline steel sample treated with Example 6, the biofilm was almost completely removed, and the green fluorescent cells were barely visible. Therefore, the biofilm thickness on the X60 pipeline steel sample treated in Application Example 8 was the lowest, indicating that the composite system of the present invention can remove the biofilm on the sample surface. The X60 pipeline steel samples corresponding to the blank control group 2 and Application Examples 4 to 6, after being cultured for 7 days, were taken out, fixed, dehydrated, sputter-coated with gold, and their surface morphology was observed using a scanning electron microscope. Figure 5 As shown; here, combined with Figure 5 The effects of the inhibitors corresponding to Examples 4 to 6 on the biofilm morphology of thermophilic sulfate archaea were analyzed. It was determined that in the blank control group, the thermophilic sulfate archaea on the surface of the X60 pipeline steel samples aggregated in large numbers in a dense spherical morphology, with the cells tightly connected by extracellular polymers to form a mature biofilm. In the X60 pipeline steel samples treated with Example 4, the density and integrity of the surface biofilm decreased, and the gaps between cells widened. In the X60 pipeline steel samples treated with Example 5, the bacterial dispersion was significantly improved, with only a small number of cells adhering isolated to the substrate surface. In the X60 pipeline steel samples treated with Example 6, the sample surface was relatively smooth, with almost no bacterial adhesion or biofilm residue observed.

[0070] The blank control group 2 and the X60 pipeline steel samples treated in Examples 4 to 6 were placed in the respective treatment systems and incubated for 7 days. After incubation, Clarke solution was used to remove corrosion products and biofilm from the surface of the X60 pipeline steel samples. After cleaning and drying, the sample mass was weighed, and the corrosion rate was calculated using the above-described formula for calculating corrosion rate. See [link to relevant documentation]. Figure 6 The results showed that the corrosion rate of the blank control group 2 was 0.100. The corrosion rate of the X60 pipeline steel sample treated in Example 4 decreased to 0.047 mm y. 1 The corrosion rate of the X60 pipeline steel sample treated in Example 5 decreased to 0.027 mm y. 1 The corrosion rate of the X60 pipeline steel sample treated in Example 6 was further reduced to 0.013 mm y. 1The lowest corrosion rate was observed. This indicates that the combination of tobacco stem extract and copper-silver nanocomposite particles has a significant synergistic inhibitory effect on the corrosion by thermophilic sulfate archaea.

[0071] The X60 pipeline steel samples treated in blank control group 2 and application examples 4 to 6 were subjected to surface corrosion products and biofilm removal. The pitting morphology of the X60 pipeline steel surface was observed using a laser confocal microscope, and the maximum pitting depth was measured. Figure 6 The effects of the inhibitors corresponding to Examples 4 to 6 on pitting corrosion induced by thermophilic sulfate archaea, along with a blank control group, were analyzed. The results showed that the maximum pitting depth of the X60 pipeline steel sample in the blank control group was 10.4 μm. The maximum pitting depth of the X60 pipeline steel sample treated with Example 4 decreased to 6.3 μm; the maximum pitting depth of the X60 pipeline steel sample treated with Example 5 decreased to 3.4 μm; and the maximum pitting depth of the X60 pipeline steel sample treated with Example 6 was only 3.1 μm. This demonstrates that the microbial corrosion compound inhibitor provided by this invention can effectively inhibit localized pitting corrosion caused by thermophilic sulfate archaea.

[0072] The blank control group 2 and the X60 pipeline steel samples treated in Examples 4 to 6 were prepared as working electrodes, with a saturated calomel electrode as the reference electrode and a platinum electrode as the counter electrode, constructing a three-electrode system. Electrochemical tests were conducted under anaerobic conditions for 7 days, including open-circuit potential, linear polarization resistance, electrochemical impedance spectroscopy, and potentiodynamic polarization measurements. Figure 7 In addition to the blank control group, the effects of the inhibitors corresponding to Examples 4 to 6 on the electrochemical behavior of high-temperature thermophilic sulfate archaeal corrosion were analyzed. It was determined that the corrosion current density of the X60 pipeline steel sample corresponding to the blank control group was 38.7 μA cm⁻¹. 2 The corrosion current density of the X60 pipeline steel sample treated in Example 4 decreased to 28.1 μA cm⁻¹. 2 The corrosion inhibition rate was 27.4%; after treatment with Example 5, the corrosion current density of the X60 pipeline steel sample decreased to 9.2 μA cm⁻¹. 2 The corrosion inhibition rate was 76.2%; after treatment with Example 6, the corrosion current density of the X60 pipeline steel sample decreased to 4.2 μA cm⁻¹. 2 The corrosion inhibition rate was 89.1%. Electrochemical impedance spectroscopy results showed that the X60 pipeline steel sample treated with Example 6 had the highest charge transfer resistance, indicating that the electrochemical corrosion reaction was significantly suppressed.

[0073] The number of free bacteria in the culture medium and the number of bacteria attached to the surface of the X60 pipeline steel samples treated with Examples 4 to 6 were counted using the hemocytometer method. Figure 8 The effects of the inhibitors corresponding to the blank control group 2 and Examples 4 to 6 on the free and sessile thermophilic sulfate archaea were analyzed. It can be determined that the number of free and sessile thermophilic sulfate archaea in the X60 pipeline steel samples treated with Examples 5 and 6 was significantly lower than that in the X60 pipeline steel samples treated with Example 4.

[0074] Sulfate-reducing bacteria (SRB) and thermophilic sulfate archaea are both typical microbial corrosion bacteria in industrial environments, but they have significantly different growth temperature ranges. SRB maintain high growth and metabolic activity primarily in low- to medium-temperature environments (approximately 20–40°C), making them the main species inducing microbial corrosion in ambient-temperature service environments such as oil and gas gathering and transportation pipelines, storage tanks, and marine engineering projects. Thermophilic sulfate archaea, on the other hand, can grow stably in high-temperature environments (approximately 60–80°C) and are widely found in deep oil and gas reservoirs, geothermal development systems, and high-temperature produced fluid treatment environments, making them important inducing microbial corrosion at high temperatures. Therefore, in actual industrial production processes, metallic materials often face the risk of being acted upon by multiple corrosive microorganisms under different temperature conditions, placing higher demands on the temperature adaptability of compound microbial corrosion inhibitors.

[0075] Although sulfate-reducing bacteria and thermophilic sulfate archaea have different suitable growth temperatures and physiological characteristics, the tobacco stem extract-copper-silver nanocomposite particles wide-temperature-range microbial corrosion inhibitor described in this invention exhibits excellent bactericidal activity, biofilm inhibition ability, and corrosion protection effect in both microbial systems. This indicates that the composite system can maintain good stability and continuous antibacterial performance under normal and high temperature environments, and has the ability to control microbial corrosion over a wide temperature range, which can meet the application requirements of different temperature conditions in complex industrial service environments.

[0076] The experimental results indicate that the main function of tobacco stem extract is not simply to directly kill microorganisms, but rather to interfere with the formation and stability of extracellular polymers through its plant-derived active ingredients. This weakens the shielding effect of mature biofilms, reduces the adhesion of corrosive microorganisms to metal surfaces, and improves the transfer efficiency of copper-silver nanocomposite particles into the biofilm. Simultaneously, tobacco stem extract can also improve the dispersion stability of copper-silver nanocomposite particles in the system, enhancing the sustained action of the composite system. The copper-silver nanocomposite particles then enter the microbial enrichment region through nanoscale effects and continuously release Cu²⁺. + and Ag +This process disrupts the integrity of microbial cell membranes, induces the generation of reactive oxygen species, and inhibits cell metabolism and genetic material function, thereby achieving highly efficient killing of corrosive microorganisms. The two components complement and synergistically inhibit both free-floating and attached bacteria simultaneously.

[0077] Therefore, the mechanism of action of the wide-temperature-range microbial corrosion compound inhibitor described in this invention is as follows: the tobacco extract first weakens the biofilm structure on the metal surface, reduces the adhesion ability of microorganisms, and promotes the diffusion of bactericidal active components into the biofilm; the copper-silver nanocomposite particles further continuously kill microorganisms inside the biofilm and liquid-phase free microorganisms by continuously releasing copper and silver ions and inducing the generation of reactive oxygen species, while inhibiting the re-attachment of microorganisms and the formation of new biofilms; ultimately reducing the biofilm coverage on the surface of the metal material, slowing down the microbial-induced corrosion process, reducing the uniform corrosion rate and the degree of local pitting corrosion, and improving the resistance of carbon steel to microbial corrosion in complex service environments.

[0078] The above results indicate that the wide-temperature-range microbial corrosion inhibition system of tobacco stem extract plus copper-silver nanocomposite particles provided by this invention can not only fully leverage the synergistic antibacterial effect between plant-derived active substances and nanomaterials, and improve the composite system's control over microbial biofilms, but also achieve simultaneous inhibition of room-temperature sulfate-reducing bacteria and thermophilic archaea. This effectively solves the technical problems of traditional bactericides such as decreased activity at high temperatures, insufficient ability to remove mature biofilms, and limited temperature adaptability. It can be widely applied in oil and gas extraction and gathering pipelines, geothermal development equipment, chemical storage and transportation equipment, and other metal service environments with wide-temperature-range microbial corrosion risks.

[0079] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the present invention. Those skilled in the art can make various modifications, substitutions, or equivalent transformations to the technical solutions of the present invention without departing from the spirit and substance of the present invention, and all such modifications, substitutions, or equivalent transformations should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A wide-temperature-range microbial corrosion inhibitor, characterized in that, It includes copper-silver nanocomposite particles and tobacco stem extract; wherein the mass content of the copper-silver nanocomposite particles is 10ppm~20ppm; and the mass content of the tobacco stem extract is 100ppm~150ppm.

2. The wide-temperature-range microbial corrosion compound inhibitor according to claim 1, characterized in that, The copper-silver nanocomposite particles have a particle size of 10nm~20nm.

3. The wide-temperature-range microbial corrosion compound inhibitor according to claim 1, characterized in that, The tobacco stem extract is an organic active ingredient extracted from tobacco stems.

4. The wide-temperature-range microbial corrosion compound inhibitor according to claim 1, characterized in that, The copper-silver nanocomposite particles have a mass content of 10 ppm; the tobacco stem extract has a mass content of 100 ppm.

5. A method for preparing a wide-temperature-range microbial corrosion compound inhibitor as described in any one of claims 1 to 4, characterized in that, include: Step 1: Dissolve the aqueous copper-silver nanocomposite particles in sterile water to obtain a copper-silver nanocomposite particle stock solution. Step 2: Dissolve the tobacco stem extract powder in sterile water or a weakly alkaline aqueous solution to obtain a tobacco stem extract stock solution; Step 3: Mix the copper-silver nanocomposite particle stock solution and the tobacco stem extract stock solution to obtain the wide-temperature-range microbial corrosion compound inhibitor; In step 3, the mass content of copper-silver nanocomposite particles in the wide-temperature-range microbial corrosion compound inhibitor is 10ppm~20ppm, and the mass content of tobacco stem extract is 100ppm~150ppm.

6. A method of using a wide-temperature-range microbial corrosion compound inhibitor as described in any one of claims 1 to 4, characterized in that, include: The surface of the substrate is brought into contact with the wide-temperature-range microbial corrosion compound inhibitor.

7. The method of using the wide-temperature-range microbial corrosion compound inhibitor according to claim 6, characterized in that, The substrate is a pipe.

8. The method of using the wide-temperature-range microbial corrosion compound inhibitor according to claim 7, characterized in that, The pipeline is made of X60 pipeline steel.

9. The application of a wide-temperature-range microbial corrosion compound inhibitor as described in any one of claims 1 to 4, characterized in that, The wide-temperature-range microbial corrosion compound inhibitor is applied within a wide temperature range of 30℃ to 70℃.

10. The application of the wide-temperature-range microbial corrosion compound inhibitor according to claim 9, characterized in that, It is used to inhibit microbial corrosion of the substrate surface caused by room-temperature sulfate-reducing bacteria and high-temperature thermophilic sulfate archaea.

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