Electrolyte beverage containing sodium hyaluronate and collagen peptide and method for preparing the same

Abstract

The application discloses an electrolyte beverage with electrolyte supplement and skin moisturizing functions and a preparation method thereof, and the electrolyte beverage comprises sodium chloride, potassium chloride, sodium citrate, potassium citrate, magnesium gluconate, zinc gluconate, sodium hyaluronate, fish collagen peptide, vitamin C, xylitol, citric acid, D-sodium erythorbate, disodium hydrogen phosphate, tea polyphenol, edible essence, potassium sorbate and purified water. Through the rapid water supplement effect of the electrolyte system and the synergistic moisturizing mechanism of sodium hyaluronate and fish collagen peptide, the application can supplement the lost water during exercise, significantly improve the skin hydration capacity and elasticity, and solve the pain point that the traditional sports beverage has single function and cannot meet the beauty demand. The preparation process is simple, the taste is refreshing, and the electrolyte beverage is suitable for daily water supplement and post-exercise recovery of healthy people.

Description

Technical Field

[0001] This invention relates to the field of functional beverage technology, specifically to a compound beverage that combines electrolyte replenishment and skin moisturizing functions, and its preparation method. Background Technology

[0002] With increasing health awareness, people's demand for beverages has shifted from simply quenching thirst to functional drinks with specific health benefits. Electrolyte drinks are widely used in situations involving sweating, such as exercise or physical labor, to replenish water and electrolytes like sodium and potassium, maintaining fluid balance and normal physiological functions. However, traditional electrolyte drinks have a single function, primarily targeting fluid replenishment, and do not offer cosmetic benefits that improve skin health.

[0003] On the other hand, skin aging is accompanied by moisture loss, decreased elasticity, and wrinkle formation. Sodium hyaluronate, as a naturally occurring moisturizing component in the human body, has attracted much attention due to its excellent water-retention capacity. In recent years, oral administration of collagen peptides and hyaluronic acid to achieve skin moisturization has become a research hotspot. For example, the clinical study by Men Yuehua et al. ("The Role of Oral Collagen Peptides and Sodium Hyaluronate in Anti-aging of Facial Skin". Chinese Journal of Medical Aesthetics, 2025, 15(07):36-42. DOI:10.19593 / j.issn.2095-0721.2025.07.007.) showed that oral administration of sodium hyaluronate can significantly increase the water content of the stratum corneum and reduce transepidermal water loss.

[0004] Currently, there is a lack of products on the market that combine electrolyte drinks that "quickly replenish body fluids and maintain hydration" with oral hyaluronic acid technology that "nourishes the skin from the inside out and achieves long-lasting hydration." Summary of the Invention

[0005] Purpose of the invention: To address the technical problems of existing electrolyte beverages that only replenish electrolytes and lack skin moisturizing and anti-aging functions, as well as the unreasonable osmotic pressure, slow hydration and absorption, poor stability of active ingredients, and unpleasant taste of existing oral beauty drinks, this invention provides a compound beverage that combines electrolyte replenishment and skin moisturizing functions, as well as its preparation method. It achieves integrated functions of rapid hydration, electrolyte balance, oral moisturizing, and firming and anti-aging, while also possessing the characteristics of good stability, refreshing taste, rapid absorption, and suitability for long-term consumption.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: an electrolyte beverage that combines post-exercise electrolyte replenishment and oral skin moisturizing functions, wherein the components are formulated as follows by mass percentage: sodium chloride 0.03-0.07%, potassium chloride 0.03-0.06%, sodium citrate 0.05-0.3%, potassium citrate 0.07-0.12%, magnesium gluconate 0.01-0.1%, zinc gluconate 0.01-0.03%, sodium hyaluronate 0.05-0.12%, fish collagen peptide 1-3%, vitamin C 0.1-0.3%, xylitol 2-4%, citric acid 0.1-0.3%, sodium D-isoascorbate 0.008-0.015%, disodium hydrogen phosphate 0.02-0.05%, tea polyphenols 0.002-0.004%, edible flavoring 0.01-0.1%, potassium sorbate 0.01-0.05%, and the balance being purified water.

[0007] Preferably, the sodium hyaluronate has a molecular weight of 400,000 to 2,000,000 Daltons.

[0008] Preferably, the fish collagen peptide is a type I small molecule fish collagen peptide derived from deep-sea fish skin, with a molecular weight ≤1000 Daltons.

[0009] Preferably, the total mass percentage of sodium citrate and potassium citrate is 0.12-0.42%.

[0010] Preferably, the osmotic pressure of the beverage is 250-330 mOsm / kg, which is an isotonic system, and it is rapidly absorbed after oral administration.

[0011] Preferably, the sodium hyaluronate has a mass percentage of 0.1-0.12%, and the fish collagen peptide has a mass percentage of 1.5-2.5%.

[0012] Preferably, the mass ratio of sodium hyaluronate to fish collagen peptide is 1:(15-20), and the combination of the two has a synergistic effect, resulting in better moisturizing and anti-aging effects. Preferably, the mass ratio of tea polyphenols to sodium D-isoascorbate is 1:4-6, and the two form a synergistic antioxidant system, significantly improving the stability of the beverage.

[0013] This invention also provides a method for preparing an electrolyte beverage that combines electrolyte replenishment and skin moisturizing functions, the preparation method comprising the following steps:

[0014] Step 1: Weigh out sodium hyaluronate, fish collagen peptides and disodium hydrogen phosphate according to the ratio, and add them together to purified water accounting for 15-25% of the total water volume. Stir at a temperature of 25-35℃ and a stirring speed of 400-600 rpm until completely dissolved to obtain solution A.

[0015] Step 2: Weigh out sodium chloride, potassium chloride, sodium citrate, potassium citrate, magnesium gluconate, and zinc gluconate according to the ratio, and add them together to purified water that accounts for 50-60% of the total water volume. Stir until completely dissolved to obtain solution B.

[0016] Step 3: Weigh xylitol, vitamin C, citric acid, potassium sorbate, sodium D-isoascorbate, tea polyphenols, and edible flavoring according to the formula. Add them to the remaining purified water, stir to dissolve, and adjust the pH of the system to 3.5-4.0 with food-grade citric acid to obtain solution C.

[0017] Step 4: Mix solution B and solution C and stir until homogeneous to obtain mixture BC; then inject solution A into mixture BC using an online homogenizer at 10-15 MPa, and continue homogenizing until the system is uniform and stable;

[0018] Step 5: Sterilize the uniformly mixed liquid. The sterilization method can be selected from the following: Method 1: Use ultra-high pressure treatment at a pressure of 400-600 MPa for 3-5 minutes. After sterilization, filter through a 0.22 μm microporous membrane and then aseptically fill. Method 2: Use pasteurization at a temperature of 65-70℃ for 20-30 minutes.

[0019] After sterilization, the pH value of the resulting beverage product is stable at 3.5-4.0, and the sodium hyaluronate and fish collagen peptides have stable structures, do not degrade, and have a high activity retention rate.

[0020] This invention is based on the design principle of rapid hydration through isotonic electrolytes and oral skin moisturizing and anti-aging mechanisms. The core design concept is to scientifically combine an electrolyte system composed of sodium, potassium, magnesium, and zinc with sodium hyaluronate and small-molecule fish collagen peptides. By controlling osmotic pressure, limiting molecular weight and ratio, constructing a pH buffer system and a dual antioxidant system, and using stepwise dissolution, online homogenization, and low-temperature mild sterilization processes, the two functions of electrolyte hydration and skin moisturizing and anti-aging are compatible and stable. This invention solves the defects of existing products, such as single function, poor absorption, easy degradation of activity, and unpleasant taste, and achieves the technical goals of multi-purpose use, high-efficiency absorption, and long-term stability.

[0021] This invention combines an isotonic electrolyte system with a sodium hyaluronate-fish collagen peptide functional system, and achieves stable activity and dual efficacy through specific ratios, synergistic antioxidant effects, precise pH control, and segmented preparation processes. Compared with existing technologies, the most critical differentiated design of this invention is that it breaks through the limitations of traditional beverages with only one function, and simultaneously achieves rapid hydration and electrolyte replenishment after exercise, as well as oral moisturizing, firming, and anti-aging effects. It takes into account absorption efficiency, taste, flavor, and shelf-life stability. The overall function, effect, and applicability are significantly superior to existing electrolyte beverages and beauty drinks.

[0022] The beneficial effects of this invention are as follows: This invention achieves rapid hydration and efficient replenishment of essential minerals such as sodium, potassium, magnesium, and zinc through an isotonic electrolyte system. It is rapidly absorbed, has a mild taste, and is particularly suitable for consumption after exercise. The scientific combination of sodium hyaluronate and small-molecule fish collagen peptides creates a synergistic effect; long-term oral consumption can significantly increase skin hydration, enhance skin elasticity, and reduce fine lines, achieving moisturizing and anti-aging from the inside out. Simultaneously, precise pH control, step-by-step dissolution, online homogenization, and gentle sterilization processes effectively protect the active ingredients such as sodium hyaluronate and fish collagen peptides from degradation. Combined with a dual antioxidant system of tea polyphenols and D-isoascorbic acid sodium, it significantly improves product stability and shelf life. Furthermore, the formula has a refreshing taste, no fishy smell, and is safe and compliant with regulations. It combines the advantages of rapid hydration and electrolyte replenishment, oral skincare, and long-term stable palatability, overcoming the shortcomings of traditional electrolyte drinks (single function) and beauty drinks (poor absorption, instability, and unpleasant taste). It has a wider range of applications and superior overall effects. Detailed Implementation

[0023] The present invention will be described in detail below with reference to specific embodiments. The illustrative embodiments and descriptions are only used to explain the present invention and are not intended to limit the present invention.

[0024] The following sources of raw materials are provided as examples:

[0025] Sodium hyaluronate: food grade, with molecular weights of 2 million Da, 1.2 million Da, and 400,000 Da, purchased from Shandong Runxin Biotechnology Co., Ltd.

[0026] Fish collagen peptides: Type I small molecule fish collagen peptides derived from food-grade deep-sea fish skin, with a molecular weight ≤1000Da, purchased from Shanxi Shuohang Biotechnology Co., Ltd.

[0027] Sodium chloride: food grade, 60 mesh, purchased from Henan Beiyi Biotechnology Co., Ltd.

[0028] Potassium chloride: food grade, 80-100 mesh, purchased from Henan Guangheng Food Ingredients Co., Ltd.

[0029] Sodium citrate: food grade, purchased from Nanjing Jiayixinwei Chemical Co., Ltd.

[0030] Potassium citrate: food grade, purchased from Jiangsu Peptide Biotechnology Co., Ltd.;

[0031] Magnesium gluconate: food grade, purchased from Jinan Dongxuan Bioengineering Co., Ltd.

[0032] Zinc gluconate: food grade, purchased from Jinan Dongxuan Bioengineering Co., Ltd.

[0033] Vitamin C: Food grade, purchased from Jinan Dongxuan Bioengineering Co., Ltd.

[0034] Xylitol: food grade, 100 mesh, purchased from Jinan Dongxuan Bioengineering Co., Ltd.;

[0035] Citric acid: food grade, purchased from Jinan Dongxuan Bioengineering Co., Ltd.

[0036] Sodium D-isoascorbate: food grade, purchased from Jinan Dongxuan Bioengineering Co., Ltd.;

[0037] Disodium hydrogen phosphate: food grade, purchased from Shandong Zhenquan Environmental Protection Technology Co., Ltd.;

[0038] Tea polyphenols: food grade, content ≥90%, purchased from Jinan Dongxuan Bioengineering Co., Ltd.

[0039] Edible flavoring: food grade, purchased from Zhejiang Haida Biotechnology Co., Ltd.;

[0040] Potassium sorbate: food grade, purchased from Jinan Dongxuan Bioengineering Co., Ltd.

[0041] Example 1

[0042] An electrolyte beverage that combines post-exercise electrolyte replenishment with oral skin moisturizing functions has the following formula by weight percentage: sodium chloride 0.05%, potassium chloride 0.04%, sodium citrate 0.15%, potassium citrate 0.10%, magnesium gluconate 0.05%, zinc gluconate 0.02%, sodium hyaluronate 0.10%, fish collagen peptide 2.0%, vitamin C 0.2%, xylitol 3.0%, citric acid 0.2%, sodium D-isoascorbate 0.01%, disodium hydrogen phosphate 0.03%, tea polyphenols 0.002%, edible flavoring 0.05%, potassium sorbate 0.03%, and the balance being purified water.

[0043] Among them, sodium hyaluronate has a molecular weight of 1.2 million Da; fish collagen peptides are type I small molecule fish collagen peptides derived from deep-sea fish skin, with a molecular weight ≤1000 Da; the mass ratio of tea polyphenols to sodium D-isoascorbate is 1:5; and the osmotic pressure of the beverage is approximately 290 mOsm / kg.

[0044] The preparation method of the electrolyte beverage that combines post-exercise electrolyte replenishment and oral skin moisturizing functions in this embodiment is as follows:

[0045] Step 1: Add sodium hyaluronate, fish collagen peptides and disodium hydrogen phosphate to purified water accounting for 20% of the total water volume, and stir at 30℃ and 500 rpm for 15 min until completely dissolved to obtain solution A;

[0046] Step 2: Add sodium chloride, potassium chloride, sodium citrate, potassium citrate, magnesium gluconate, and zinc gluconate to purified water that accounts for 55% of the total water volume, and stir at room temperature for 10 minutes until completely dissolved to obtain solution B;

[0047] Step 3: Add xylitol, vitamin C, citric acid, potassium sorbate, sodium D-isoascorbate, tea polyphenols, and edible flavoring to the remaining 25% purified water, stir for 8 minutes until completely dissolved, and adjust the pH of the system to 3.8 with food-grade citric acid to obtain solution C;

[0048] Step 4: Mix solution B and solution C, stir for 5 minutes until homogeneous to obtain mixture BC; then inject solution A into mixture BC using an online homogenizer at a pressure of 12 MPa, and continue homogenizing for 5 minutes until the system is homogeneous and stable.

[0049] Step 5: Sterilize the well-mixed liquid under ultra-high pressure, controlling the pressure at 500 MPa and the holding time at 4 min; after sterilization, filter the liquid through a 0.22 μm microporous membrane and then aseptically fill it to obtain the finished beverage.

[0050] Example 2

[0051] An electrolyte beverage that combines post-exercise electrolyte replenishment with oral skin moisturizing functions has the following formula by weight percentage: sodium chloride 0.03%, potassium chloride 0.06%, sodium citrate 0.05%, potassium citrate 0.12%, magnesium gluconate 0.01%, zinc gluconate 0.01%, sodium hyaluronate 0.05%, fish collagen peptide 1.5%, vitamin C 0.1%, xylitol 2.0%, citric acid 0.1%, sodium D-isoascorbate 0.008%, disodium hydrogen phosphate 0.02%, tea polyphenols 0.002%, edible flavoring 0.01%, potassium sorbate 0.01%, and the balance being purified water.

[0052] Among them, sodium hyaluronate has a molecular weight of 400,000 Da; fish collagen peptides are type I small molecule fish collagen peptides derived from deep-sea fish skin, with a molecular weight ≤1000 Da; the mass ratio of tea polyphenols to sodium D-isoascorbate is 1:4; and the osmotic pressure of the beverage is approximately 250 mOsm / kg.

[0053] Preparation method: Steps one to four are the same as in Example 1; Step five: pasteurize, keep warm at 65℃ for 30 minutes, cool and fill to obtain the finished product.

[0054] Example 3

[0055] An electrolyte beverage that combines post-exercise electrolyte replenishment with oral skin moisturizing functions has the following formula by weight percentage: sodium chloride 0.07%, potassium chloride 0.03%, sodium citrate 0.3%, potassium citrate 0.07%, magnesium gluconate 0.1%, zinc gluconate 0.03%, sodium hyaluronate 0.12%, fish collagen peptide 2.5%, vitamin C 0.3%, xylitol 4.0%, citric acid 0.3%, sodium D-isoascorbate 0.015%, disodium hydrogen phosphate 0.05%, tea polyphenols 0.004%, edible flavoring 0.1%, potassium sorbate 0.05%, and the balance being purified water.

[0056] Among them, sodium hyaluronate has a molecular weight of 2 million Da; fish collagen peptides are type I small molecule fish collagen peptides derived from deep-sea fish skin, with a molecular weight ≤1000 Da; the mass ratio of tea polyphenols to sodium D-isoascorbate is 1:6; and the osmotic pressure of the beverage is approximately 330 mOsm / kg.

[0057] Preparation method: Steps one to four are the same as in Example 1; Step five: Sterilize at 600MPa for 3 minutes using ultra-high pressure, filter through a 0.22μm membrane and aseptically fill to obtain the finished product.

[0058] Comparative Example 1

[0059] Compared to Example 1, sodium hyaluronate was not added, but the remaining components, proportions, and preparation methods were exactly the same.

[0060] Comparative Example 2

[0061] Compared to Example 1, no fish collagen peptides were added, but the remaining components, proportions, and preparation methods were exactly the same.

[0062] Comparative Example 3

[0063] Compared to Example 1, a one-step mixing process (all raw materials are directly mixed and dissolved) is used, without stepwise dissolution and online homogenization, but otherwise the process is the same.

[0064] Comparative Example 4

[0065] Commercially available ordinary electrolyte drinks.

[0066] Test case

[0067] 1. Sensory quality evaluation experiment

[0068] 1) Experimental objective: To verify whether the taste, flavor, smoothness, and acceptability of this invention meet the standards for high-quality beverages.

[0069] 2) Experimental method: 20 professional sensory evaluators conducted a blinded test and scored the taste, off-flavor, smoothness and overall acceptability (out of 10 points). The results are shown in Table 1.

[0070] Table 1 Sensory quality evaluation results

[0071]

[0072] As can be seen from Table 1, the sensory quality of Examples 1-3 of the present invention is significantly better than that of the comparative examples. The taste is sweet and refreshing, without fishy or off-flavors, and smooth and palatable. The step-by-step dissolution, online homogenization and precise pH control process can effectively mask the unpleasant odors brought by functional ingredients, improve the drinking experience and make it more suitable for long-term daily consumption.

[0073] 2. Test on the effects of rapid thirst quenching and electrolyte replenishment

[0074] 1) Experimental objective: To verify whether the present invention has the effect of quickly quenching thirst and quickly restoring electrolyte balance, and to prove that it is suitable for drinking after exercise.

[0075] 2) Experimental method: Twenty healthy adults were selected and exercised until they were sweating and dehydrated. They were randomly divided into two groups and drank the beverage of Example 1 and the beverage of Comparative Example 4 (commercially available electrolyte beverage) respectively. The time of thirst quenching was recorded. The recovery of blood sodium and potassium ion levels was measured at 25 min and 45 min after drinking to determine the time for electrolytes to return to normal. The results are shown in Table 2.

[0076] Table 2 Comparison of thirst quenching speed and electrolyte replenishment efficiency

[0077]

[0078] As can be seen from Table 2, the present invention quenches thirst faster, has higher electrolyte absorption efficiency, can restore body fluid balance in a short time, relieve fatigue and dry mouth, and the comfort brought by the isotonic system is significantly better than that of commercially available hypertonic electrolyte beverages.

[0079] 3. Experiment on skin moisturizing and improvement of skin hydration status

[0080] 1) Experimental objective: To verify the effects of this invention in increasing skin moisture content, reducing transepidermal water loss, enhancing skin elasticity, and improving dryness and roughness through oral administration, and to prove that it also has the functions of beautifying and nourishing the skin.

[0081] 2) Experimental Methods: Thirty healthy subjects (with dry skin and weak elasticity) were selected and consumed 500 mL of the beverages from Examples 1 and Comparative Examples 2 and 4 daily for 28 consecutive days. The following parameters were measured using a skin testing instrument: ① stratum corneum water content; ② transepidermal water loss (TEWL); ③ skin elasticity parameter R². The experimental data represent the rate of change compared to day 0. The results are shown in Table 3.

[0082] Table 3. Effects of skin moisturizing, anti-aging, and improvement of hydration status

[0083]

[0084] As can be seen from Table 3, continuous consumption of the beverage of the present invention for 28 days can significantly improve skin moisture, strengthen the skin barrier, and enhance elasticity, confirming that it has clear oral moisturizing, beautifying, skin hydration improvement, and anti-aging effects. Moreover, the effect is significantly better than that of the beverages of Comparative Example 1 (without sodium hyaluronate) and Comparative Example 2 (without fish collagen peptides), proving that the combination of sodium hyaluronate and fish collagen peptides in the system of the present invention produces a significant synergistic effect in skin moisturizing, anti-aging, and hydration improvement.

[0085] 4. Stability test of active ingredients

[0086] 1) Experimental objective: To verify the protective effect of the process of the present invention on sodium hyaluronate and fish collagen peptides, and to prove that they are not easily degraded and have a high activity retention rate.

[0087] 2) Experimental method: Example 1 and Comparative Example 3 (one-step mixing process) were placed in an environment of 37°C and 75% RH for 90 days, and the remaining contents of sodium hyaluronate and fish collagen peptides were measured. The results are shown in Table 4.

[0088] Table 4. Retention rate of active ingredients in accelerated experiments after 90 days

[0089] Example 1 92.3% 90.1% Clear, odorless, and without fishy smell Comparative Example 3 72.1% 68.3% It has a layered structure, a distinct fishy smell, and poor stability.

[0090] As can be seen from Table 4, the present invention employs a stepwise dissolution, online homogenization, precise pH control, and gentle sterilization process, which can significantly protect sodium hyaluronate and fish collagen peptides, avoiding degradation, denaturation, and fishy odor. Its stability is far superior to that of conventional one-step mixing processes.

[0091] 5. Physicochemical properties and isotonicity test

[0092] 1) Experimental objective: To verify whether the osmotic pressure, pH, solubility, and long-term stability of the beverage of this invention meet the requirements of isotonic functional beverages, so as to prove that it is absorbed quickly, does not harm the stomach and intestines, and has stable quality.

[0093] 2) Experimental methods: Take samples from Examples 1-3 and Comparative Examples 1-4, measure the osmotic pressure using an osmometer, measure the pH value using a pH meter, and observe the dissolution state; place them at 37℃ and 75% relative humidity for 90 days, and observe whether precipitation, layering, turbidity, or odor occurs. The results are shown in Table 5.

[0094] Table 5 Comparison of Physicochemical Indicators and Stability

[0095]

[0096] As can be seen from Table 5, the osmotic pressure of Examples 1–3 of the present invention is all in the isotonic range of 250-330 mOsm / kg, which is close to the osmotic pressure of human body fluids, making it more conducive to rapid absorption and not increasing the burden on the gastrointestinal tract; the pH is stable in the optimal range of 3.5-4.0; the solubility is good, the system is uniform, and the stability after 90 days is excellent, which is significantly better than the one-step mixing process (Comparative Example 3) and commercially available hypertonic electrolyte beverages (Comparative Example 4).

[0097] 6. Antioxidant activity and free radical scavenging ability test

[0098] 1) Experimental objective: To verify the scavenging ability of the beverage of this invention against DPPH free radicals, hydroxyl free radicals, and ABTS free radicals, and to prove that the formula has the effects of anti-oxidation, delaying skin aging, and protecting active ingredients.

[0099] 2) Experimental method: The scavenging rates of DPPH free radical, hydroxyl free radical and ABTS free radical of Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 4 were measured respectively. Each group was repeated in 3 parallel trials and the average value was taken. The results are shown in Table 6.

[0100] Table 6 Comparison of antioxidant and free radical scavenging capabilities

[0101]

[0102] As can be seen from Table 6, the beverage of the present invention has a significantly higher free radical scavenging rate and excellent antioxidant activity. Sodium hyaluronate, fish collagen peptide, vitamin C, tea polyphenols, and sodium D-isoascorbate form a synergistic antioxidant system, which can effectively scavenge free radicals in the body, reduce oxidative damage, delay skin aging, and improve the stability of the beverage. Moreover, the effect is significantly better than that of the beverages of Comparative Example 1 (without sodium hyaluronate) and Comparative Example 2 (without fish collagen peptide), proving that in the system of the present invention, the combination of sodium hyaluronate and fish collagen peptide has a significant synergistic effect on the scavenging rate of DPPH free radicals, hydroxyl free radicals, and ABTS free radicals.

[0103] 7. Intestinal absorption and bioavailability experiment

[0104] 1) Experimental objective: To verify the oral absorption efficiency of sodium hyaluronate and fish collagen peptides in the beverage of this invention, and the ability of the active ingredients to accumulate in skin tissue, so as to provide pharmacokinetic basis for the oral moisturizing and anti-aging effects.

[0105] 2) Experimental Methods: Thirty healthy SD rats were randomly divided into three groups: Example 1 group, Comparative Example 1 group (without sodium hyaluronate), and Comparative Example 2 group (without fish collagen peptides). The rats were administered the drug by gavage at a dose of 10 mL / kg. Blood samples were collected from the abdominal aorta at 2 h and 6 h after administration, and plasma was separated. Skin tissue from the back was collected and homogenized. The concentration of hyaluronic acid in the plasma was determined by high-performance liquid chromatography (HPLC), and the content of hydroxyproline (a characteristic marker of collagen metabolism) in the skin tissue was determined by spectrophotometry. Each group underwent six parallel measurements, and the average value was taken. The results are shown in Table 7.

[0106] Table 7. Results of determination of hyaluronic acid content in rat plasma and hydroxyproline content in skin.

[0107]

[0108] Note: ND indicates not detected.

[0109] As shown in Table 7, after oral administration of the beverage of Example 1 to rats, the concentration of hyaluronic acid in plasma increased significantly over time, and the content of hydroxyproline in skin tissue increased significantly. This indicates that the sodium hyaluronate in this invention can be effectively absorbed into the bloodstream after oral administration, and the fish collagen peptides can be decomposed, absorbed, and enriched and deposited in skin tissue. Compared with Comparative Examples 1 and 2, the absorption efficiency and skin-targeted enrichment effect of the Example 1 group are better, which confirms that the combination of sodium hyaluronate and fish collagen peptides can significantly synergistically improve bioavailability and provide direct data support for the skin moisturizing and anti-aging effects.

[0110] In summary, through seven systematic experiments, this invention has demonstrated that the formulated electrolyte beverage is scientifically sound and manufactured using a stable process. It combines rapid hydration and electrolyte replenishment after exercise, long-lasting oral moisturizing and anti-aging effects, high stability, and excellent sensory quality. It exhibits high retention rate of active ingredients, strong antioxidant capacity, and excellent absorption and utilization efficiency, achieving dual benefits of exercise recovery and skin care. Its overall performance is significantly superior to existing products, demonstrating promising application prospects.

Claims

1. An electrolyte beverage that combines electrolyte replenishment and skin moisturizing functions, characterized in that: The composition of the components by mass percentage is as follows: sodium chloride 0.03-0.07%, potassium chloride 0.03-0.06%, sodium citrate 0.05-0.3%, potassium citrate 0.07-0.12%, magnesium gluconate 0.01-0.1%, zinc gluconate 0.01-0.03%, sodium hyaluronate 0.05-0.12%, fish collagen peptide 1-3%, vitamin C 0.1-0.3%, xylitol 2-4%, citric acid 0.1-0.3%, sodium D-isoascorbate 0.008-0.015%, disodium hydrogen phosphate 0.02-0.05%, tea polyphenols 0.002-0.004%, edible flavoring 0.01-0.1%, potassium sorbate 0.01-0.05%, with the balance being purified water.

2. The electrolyte beverage according to claim 1, characterized in that, The molecular weight of the sodium hyaluronate is 400,000 to 2,000,000 Daltons.

3. The electrolyte beverage according to claim 1, characterized in that, The fish collagen peptides are type I small molecule fish collagen peptides derived from deep-sea fish skin, with a molecular weight ≤1000 Daltons.

4. The electrolyte beverage according to claim 1, characterized in that, The sodium hyaluronate content is 0.1-0.12%, and the fish collagen peptide content is 1.5-2.5%; the mass ratio of sodium hyaluronate to fish collagen peptide is 1:(15-20).

5. The electrolyte beverage according to claim 1, characterized in that, The mass ratio of tea polyphenols to sodium D-isoascorbate is 1:4-6.

6. The electrolyte beverage according to claim 1, characterized in that, The osmotic pressure of the beverage is 250-330 mOsm / kg.

7. A method for preparing an electrolyte beverage according to any one of claims 1-6, characterized in that, Includes the following steps: Step 1: Dissolve sodium hyaluronate, fish collagen peptides and disodium hydrogen phosphate in purified water with a total water volume of 15-25%, and stir at 400-600 rpm at 25-35℃ to form solution A; Step 2: Dissolve sodium chloride, potassium chloride, sodium citrate, potassium citrate, magnesium gluconate, and zinc gluconate in purified water that accounts for 50-60% of the total water volume to form solution B; Step 3: Dissolve xylitol, vitamin C, citric acid, potassium sorbate, sodium D-isoascorbate, tea polyphenols, and edible flavoring in the remaining purified water, and adjust the pH to 3.5-4.0 with food-grade citric acid to form solution C; Step 4: Inject solution A into the B+C mixture at 10-15 MPa using an online homogenizer; Step 5: Sterilize under ultra-high pressure at 400-600MPa for 3-5 minutes, then aseptically fill.

8. The preparation method according to claim 7, characterized in that, The sterilization process was replaced by pasteurization at 65-70℃ for 20-30 minutes; the pH value of the sterilized beverage was 3.5-4.0, and the sodium hyaluronate and fish collagen peptides remained stable.

9. The preparation method according to claim 7, characterized in that, After sterilization, the product is bottled and filtered through a 0.22μm membrane before bottling.

10. The use of an electrolyte beverage according to any one of claims 1-6 in post-exercise hydration and / or oral skin moisturizing.

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