A mineral supplement composition for fasting exercise weight loss and use thereof

Abstract

The application provides a mineral supplement composition for fasting exercise weight loss and application thereof, and belongs to the technical field of sports nutrition.The mineral supplement composition for fasting exercise weight loss provided by the application contains composition 1 composed of mineral elements potassium, calcium, vanadium, zinc, selenium, magnesium and cobalt and composition 2 composed of manganese, copper and iron.The mineral supplement composition provided by the application achieves the purpose of double precise supplement of the minerals lost in fasting exercise and the minerals chronically deficient in high-fat diet, can supplement the six kinds of minerals lost in fasting exercise acutely and the nine kinds of minerals chronically deficient in high-fat diet at the same time, and is comprehensive.After taking the mineral supplement composition provided by the application, electrolyte balance can be restored, insulin sensitivity can be improved, and fat oxidation can be promoted, thereby assisting the exercise weight loss effect and improving metabolism, and the mineral supplement composition is suitable for fasting exercise people, and is particularly suitable for weight losers with high-fat / high-energy diet.

Description

Technical Field

[0001] This invention belongs to the field of sports nutrition technology, specifically relating to a mineral supplement composition for weight loss through fasted exercise and its application. Background Technology

[0002] Obesity can negatively impact health, increasing the risk of metabolic syndrome, diabetes, and cardiovascular disease. Exercise is a common intervention for weight loss, with morning exercise on an empty stomach being particularly popular due to its perceived ability to promote fat oxidation. However, those who exercise on an empty stomach face the dual challenge of acute mineral loss due to exercise itself, coupled with chronic mineral deficiency caused by a long-term high-fat / high-energy diet. Existing exercise supplements and weight loss products are not designed to address both types of mineral loss simultaneously and lack sodium restriction. Therefore, developing a composition that can replenish minerals lost during fasting exercise, correct chronic deficiencies caused by a high-fat diet, and simultaneously restrict sodium intake is of significant practical importance. Summary of the Invention

[0003] In view of this, the purpose of the present invention is to provide a mineral supplement composition that can precisely replenish minerals lost during fasting exercise and minerals chronically deficient in a high-fat diet. It can effectively assist in weight loss through exercise and improve metabolism, and is suitable for people who exercise on an empty stomach, and is especially suitable for those who are trying to lose weight while on a high-fat / high-energy diet.

[0004] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a mineral supplement composition for weight loss through fasted exercise, comprising composition 1 and composition 2; composition 1 contains the following mineral elements: potassium, calcium, vanadium, zinc, selenium, magnesium, and cobalt; composition 2 contains the following mineral elements: manganese, copper, and iron; the mineral supplement composition is sodium-free.

[0005] Preferably, based on a single daily dose, the content of each mineral element is as follows: potassium 200~2100 mg, calcium 120~900 mg, vanadium 0.5~12 μg, manganese 0.3~2.8 mg, zinc 1.5~14 mg, selenium 2~42 μg, copper 0.2~1.8 mg, magnesium 50~340 mg, cobalt 0.015~0.19 μg, and iron 2.1~16 mg.

[0006] Preferably, the compound sources of the mineral element potassium include potassium gluconate, potassium citrate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, or potassium chloride; the compound sources of the mineral element calcium include calcium carbonate, calcium gluconate, calcium citrate, L-calcium lactate, calcium hydrogen phosphate, calcium chloride, calcium phosphate, glycerol calcium phosphate, calcium oxide, or calcium sulfate; the compound sources of the mineral element vanadium include vanadium sulfate or ethyl maltol vanadium sulfate; the compound sources of the mineral element manganese include manganese sulfate, manganese chloride, manganese carbonate, manganese citrate, or manganese gluconate; and the compound sources of the mineral element zinc include zinc sulfate, glucose... Zinc sulfate, zinc oxide, zinc lactate, zinc citrate, zinc chloride, or zinc acetate; sources of the mineral element selenium include sodium selenate or sodium selenite; sources of the mineral element copper include copper sulfate, copper gluconate, copper citrate, or copper carbonate; sources of the mineral element magnesium include magnesium sulfate, magnesium chloride, magnesium oxide, magnesium carbonate, magnesium hydrogen phosphate, magnesium citrate, or magnesium gluconate; sources of the mineral element cobalt include cyanocobalamin; sources of the mineral element iron include ferrous sulfate, ferrous gluconate, ferric ammonium citrate, ferrous fumarate, ferric citrate, or ferric pyrophosphate.

[0007] Preferably, it is taken more than 1 hour after the end of fasting exercise, and Composition 1 and Composition 2 are taken separately.

[0008] Preferably, the fasting exercise is exercise lasting ≥30 minutes and the heart rate reaches more than 60% of the maximum human heart rate.

[0009] Preferably, the dosage form of the mineral supplement composition includes powder, effervescent tablet, oral liquid, or chewable tablet.

[0010] The present invention also provides the use of the above-mentioned mineral supplement composition in the preparation of products that assist in weight loss through exercise, replenish minerals lost during exercise, and / or correct mineral deficiencies caused by a high-fat diet.

[0011] This invention also provides a method for assisting weight loss through fasting exercise, comprising the following steps: Take the above-mentioned mineral supplement combination at least one hour after completing fasted exercise each day. Combination 1 and Combination 2 should be taken separately.

[0012] Preferably, the duration of the fasting exercise is ≥30 minutes and the heart rate reaches more than 60% of the maximum human heart rate.

[0013] Preferably, the daily dietary sodium intake should be controlled to <2000 mg / day for at least 8 weeks.

[0014] The beneficial effects of this invention are: The mineral supplement composition provided by this invention achieves dual and precise replenishment of minerals lost during fasting exercise and those chronically deficient due to a high-fat diet. It can simultaneously replenish six minerals (K, Ca, V, Mn, Zn, Se) lost acutely during fasting exercise and nine minerals (V, Mn, Cu, Mg, Zn, K, Ca, Co, Se) chronically deficient due to a high-fat diet, providing comprehensive coverage. After taking the mineral supplement composition provided by this invention, electrolyte balance can be restored, insulin sensitivity improved, and fat oxidation promoted, thereby assisting in weight loss through exercise and improving metabolism. It is suitable for people exercising on an empty stomach, and is particularly suitable for those trying to lose weight while following a high-fat / high-energy diet. The mineral supplement composition provided by this invention does not contain added sodium, meeting the salt control needs of people trying to lose weight. The mineral supplement composition provided by this invention is convenient to use; it can be prepared and consumed immediately after exercise, resulting in good compliance. Attached Figure Description

[0015] Figure 1 The figures show the organ weight and organ coefficient of mice, where A represents the comparison of organ weight and B represents the comparison of organ coefficient. P <0.05, P <0.01, P <0.001, P <0.0001.

[0016] Figure 2 Principal component analysis score plots for different tissues and biological samples.

[0017] Figure 3 Heatmaps showing the content of 15 minerals in different tissues and samples.

[0018] Figure 4 Correlation analysis of mineral content and glucose and lipid metabolism indicators: A shows the correlation analysis results between minerals and TC, LDL-C, and HDL-C; B shows the correlation analysis results between minerals and GLU; blue represents negative correlation and red represents positive correlation.

[0019] Figure 5 The correlation analysis shows the relationship between mineral content and body weight and fat mass. Blue indicates a negative correlation, while red indicates a positive correlation. Detailed Implementation

[0020] This invention provides a mineral supplement composition for weight loss through fasted exercise, comprising composition 1 and composition 2; composition 1 contains the following mineral elements: potassium, calcium, vanadium, zinc, selenium, magnesium, and cobalt; composition 2 contains the following mineral elements: manganese, copper, and iron; the mineral supplement composition is sodium-free.

[0021] In a preliminary randomized controlled trial (34 healthy subjects, jumping rope for 40 minutes on an empty stomach for 3 consecutive days), this invention found that serum potassium (K), calcium (Ca), vanadium (V), manganese (Mn), zinc (Zn), and selenium (Se) levels were significantly reduced after fasting exercise (P<0.05). Failure to replenish these levels promptly may lead to electrolyte imbalances, exercise-induced fatigue, and metabolic abnormalities. Furthermore, animal experiments (C57bl / 6j mice, high-fat, high-cholesterol diet intervention for 12 weeks) revealed that a high-fat diet resulted in widespread loss of vanadium (V), manganese (Mn), copper (Cu), magnesium (Mg), zinc (Zn), potassium (K), calcium (Ca), cobalt (Co), and selenium (Se) in the body (reduced intake, decreased absorption, increased excretion), and the degree of loss was significantly positively correlated with body weight, fat mass, blood glucose, and elevated blood lipids (P<0.05). Large-scale population surveys (NHANES 2007-2018, n=16949) further confirmed that in high-energy-intake populations, low intake of magnesium (Mg), potassium (K), copper (Cu), iron (Fe), and zinc (Zn) was significantly associated with obesity risk (OR=1.35-2.08), and high sodium intake increased the risk of obesity. Therefore, this invention has developed a mineral supplement composition that can replenish minerals lost during fasting exercise, correct chronic mineral deficiencies caused by high-fat / high-energy diets, and limit sodium intake. This composition can effectively assist in weight loss through exercise and improve metabolism, and is suitable for people exercising on an empty stomach, especially those trying to lose weight while on a high-fat / high-energy diet.

[0022] In the mineral supplement composition of the present invention, the preferred content of each mineral element, based on a single daily dose, is as follows: potassium 200-2100 mg, calcium 120-900 mg, vanadium 0.5-12 μg, manganese 0.3-2.8 mg, zinc 1.5-14 mg, selenium 2-42 μg, copper 0.2-1.8 mg, magnesium 50-340 mg, cobalt 0.015-0.19 μg, iron 2.1-16 mg; more preferably, potassium 400-1000 mg, calcium 200-800 mg, vanadium 2-10 μg, manganese 1-2.5 mg, zinc 2-12 mg, selenium 5-40 μg, copper 0.5-1.5 mg, magnesium 70-330 mg, cobalt 0.02-0.17 μg, iron 3-14 mg; and even more preferably, potassium 1000-1200 mg, calcium 600 mg, vanadium 5 μg, manganese 2-3 μg. mg, zinc 10~12 mg, selenium 30 μg, copper 0.5 mg, magnesium 230~250 mg, cobalt 0.085~0.17 μg, iron 6 mg.

[0023] In this invention, the preferred sources of potassium compounds include potassium gluconate, potassium citrate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, or potassium chloride; the preferred sources of calcium compounds include calcium carbonate, calcium gluconate, calcium citrate, L-calcium lactate, calcium hydrogen phosphate, calcium chloride, calcium phosphate, glycerol calcium phosphate, calcium oxide, or calcium sulfate; the preferred sources of vanadium compounds include vanadium oxysulfate or ethyl maltol oxysulfate; the preferred sources of manganese compounds include manganese sulfate, manganese chloride, manganese carbonate, manganese citrate, or manganese gluconate; and the preferred sources of zinc compounds include zinc sulfate, potassium gluconate, potassium citrate, potassium dihydrogen phosphate ... The preferred sources of the compounds for the mineral element selenium include zinc gluconate, zinc oxide, zinc lactate, zinc citrate, zinc chloride, or zinc acetate; the preferred sources of the compounds for the mineral element copper include copper sulfate, copper gluconate, copper citrate, or copper carbonate; the preferred sources of the compounds for the mineral element magnesium include magnesium sulfate, magnesium chloride, magnesium oxide, magnesium carbonate, magnesium hydrogen phosphate, magnesium citrate, or magnesium gluconate; the preferred sources of the compounds for the mineral element cobalt include cyanocobalamin; and the preferred sources of the compounds for the mineral element iron include ferrous sulfate, ferrous gluconate, ferric ammonium citrate, ferrous fumarate, ferric citrate, or ferric pyrophosphate. This invention does not specifically limit the source of the above compounds; commercially available products conventional in the art can be used.

[0024] The mineral supplement composition of this invention is preferably taken at least one hour after exercise on an empty stomach, with Composition 1 and Composition 2 taken separately. In this invention, the exercise on an empty stomach is preferably for a duration of ≥30 minutes and a heart rate reaching at least 60% of the maximum human heart rate. In this invention, the dosage form of the mineral supplement composition preferably includes powder, effervescent tablets, oral liquid, or chewable tablets, for convenient immediate consumption after exercise. This invention does not specifically limit the preparation method for each dosage form. When preparing the mineral supplement composition of this invention into different dosage forms, it is preferable to add excipients, preferably including maltodextrin, citric acid, sweeteners, etc. When taking the mineral supplement composition of this invention, it is preferably taken once daily on exercise days, and on non-exercise days, it can be omitted or the dosage halved.

[0025] This invention also provides the application of the above-described mineral supplement composition in the preparation of products that assist in weight loss through exercise, replenish minerals lost during exercise, and / or correct mineral deficiencies caused by a high-fat diet. In this invention, the products preferably include health supplements, foods, and pharmaceuticals.

[0026] This invention also provides a method for assisting weight loss through fasting exercise, comprising the following steps: Take the above-mentioned mineral supplement combination at least one hour after completing fasted exercise each day. Combination 1 and Combination 2 should be taken separately.

[0027] In the method described in this invention, the duration of the fasting exercise is preferably ≥30 minutes and the heart rate reaches more than 60% of the maximum human heart rate; more preferably, the daily dietary sodium intake should be controlled to <2000 mg / day; and this should be continued for at least 8 weeks.

[0028] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0029] Unless otherwise specified, the following embodiments are all conventional methods.

[0030] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.

[0031] Example 1 A high-fat, high-cholesterol diet leads to an imbalance of minerals in the body. 1. Experimental Methods 1.1 Animal Experiments Sixteen 10-week-old male C57bl / 6j mice were randomly divided into two groups after one week of acclimatization: a high-fat, high-cholesterol diet (HFCD) group (15.8% fat, 1.25% cholesterol, n=8) and a control group (10% fat, 0% cholesterol, n=8). The high-fat, high-cholesterol diet and the control diet were respectively provided with free access to the former. Feed intake and uneaten food were recorded daily at fixed times to calculate food consumption. Both diets were purchased from Beijing Huafukang Biotechnology Co., Ltd. Animals were housed in an SPF-grade animal facility, maintained at a temperature of 23±1℃, humidity of 50-60%, and a 12-hour light / dark cycle, with free access to deionized water, for 12 weeks. All experimental procedures were conducted in accordance with the "Guidelines for the Care and Use of Laboratory Animals" of Harbin Medical University (Harbin, China) and were approved by the Animal Ethics Committee of Harbin Medical University.

[0032] 1.2 Sample Collection and Pretreatment Before the end of the animal experiments, fecal samples were collected from rats over 24 hours. After euthanizing the rats, their weight was recorded. Serum and 22 tissue samples, including those from the liver, spleen, and kidneys, were collected. All samples underwent microwave digestion pretreatment: 100 mg of feed, feces, and tissue samples (100 μL of serum) were accurately weighed and placed in a microwave digestion vessel. 6 mL of concentrated nitric acid was added, and digestion was performed according to the standard operating procedure of the microwave digester. After digestion, the digestion vessel was placed on a 180°C heated plate to remove acid until the digestate was clear. The solution was then cooled to room temperature. The solution was diluted with deionized water, vortexed, and then filtered before being stored at 4°C for analysis.

[0033] 1.3 Measurement of Biochemical Indicators The serum levels of TC, TG, LDL-C, GLU, high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alanine aminotransferase / aspartate aminotransferase (ALT / AST), uric acid (UA), blood urea nitrogen (BUN), creatinine (CRE), and serum calcium (Ca) were measured using a fully automated biochemical analyzer (Hitachi-7100, Japan).

[0034] 1.4 Quantitative analysis of 21 minerals by ICP-MS and methodological evaluation The concentrations of 21 minerals in mouse samples were determined using an Agilent 7800 Inductively Coupled Plasma Mass Spectrometer (ICP-MS, Thermo Fisher) from the Heilongjiang Provincial Center for Disease Control and Prevention. Before formal sampling, the ICP-MS instrument's detection conditions were optimized using tuning solutions (Li, Y, Ce, Tl). External standard method was used for sample concentration quantification, and calibration standard solution (No. 5183-4688) was purchased from Agilent Technologies. During sample detection, an ICP-MS internal standard mixture (100 μg / L, Sc45, Ge72, Rh103, Tb159, Lu172, Bi209) was introduced to correct for matrix interference.

[0035] To verify the accuracy of the method, the certified reference material Trace Elements Serum L-1 RUO was used, and the tests were performed according to the above-described pretreatment and quantitative analysis steps. The results were close to the reference values. To evaluate the recovery rate of the measurement method, a 5% nitric acid solution was used as a blank and divided into four equal parts. Three of these parts were spiked with mixed standard solutions of the analytes at low, medium, and high concentrations, respectively, with six parallel samples for each concentration. The other unspecified blank sample was also measured six times to obtain the background concentration. The recovery rate was calculated using the formula: Recovery rate = (Spiked concentration - Unspecified concentration) / Spiked concentration × 100%. To evaluate the precision of the detection of 15 minerals, three concentration samples (low, medium, and high) were prepared within the effective linear range of the standard curve, with six parallel samples for each concentration, and the relative standard deviation (RSD) of the results for each concentration was calculated.

[0036] 1.5 Data Processing and Analysis Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models were used in SIMCA-P (Version 14.1, Umetrics) software to assess the clustering trend between groups. To evaluate the robustness of the PLS-DA model, randomization and permutation tests were performed. Data were analyzed using SPSS software (version 24.0, Chicago, IL, USA), and results are expressed as mean ± standard deviation. Independent samples t-tests were used to analyze differences between the two groups. For values ​​that did not meet the parametric test requirements, nonparametric tests were used for comparison. All statistical tests were two-tailed, and p < 0.05 was considered statistically significant. The correlation between tissue mineral concentrations and serum biochemical indicators was calculated using Pearson correlation coefficient or Spearman rank correlation coefficient.

[0037] 2. Results 2.1 Food intake, body weight, and blood biochemical indicators As shown in Table 1, compared with the CON group, the HFCD group mice had significantly higher food intake, body weight, serum TC, HDL-C, LDL-C, and GLU levels. P <0.05), BUN and AST / ALT levels were significantly reduced ( P <0.05). For example... Figure 1 As shown, compared with the CON group, the HFCD group mice had significantly increased weights of liver, greater omentum fat, perirenal fat, peritesticular fat, subcutaneous fat, and brown fat. P <0.05), significantly increased reticulum fat, perirenal fat, peritesticular fat, and cardiac organ coefficient ( P <0.05).

[0038] Table 1. Food intake, body weight, and serum biochemical indicators of mice.

[0039] 2.2 Effects of HFCD on the mineral composition of 24 tissues like Figure 2 As shown in A, nine tissues (feces, cecal contents, colon, subcutaneous fat, perirenal fat, femur, prostate, brain, and spleen) of HFCD mice showed a significant separation trend from the CON group, indicating that HFCD caused significant changes in the mineral composition of these tissues, with a total of 15 mineral concentrations significantly altered. Figure 3 ).

[0040] like Figure 2 As shown in B, there were no differences in mineral composition among the 15 rat tissues (serum, liver, lung, pancreas, heart, kidney, stomach, testis, duodenum, peritesticular fat, greater omentum fat, brown fat, hair, skin, and skeletal muscle), but the concentrations of 15 minerals in these 15 tissues were significantly altered. Figure 3 ).

[0041] 2.3 Effects of HFCD on mineral intake in mice As shown in Table 2, the contents of Na, K, Ca, Mn, Fe, Cu, and Zn in the HFCD group feed were significantly lower than those in the CON group ( P <0.05%. Compared with the CON group, the intake of Na, K, and Cu minerals was significantly reduced in the HFCD group ( P <0.05), other minerals showed no difference ( P >0.05).

[0042] Table 2. Mineral content in mouse diet and daily mineral intake of mice

[0043] Note: P <0.05 2.4 Correlation analysis of mineral levels in liver and adipose tissue with glucose and lipid metabolism indicators, body weight, and fat mass like Figure 4 As shown in Figure A, the contents of 14 minerals (Cu, V, Mg, Ca, Se, Na, K, Mn, Fe, Co, Zn, Mo, Ba, Pb) in the liver, heart, perirenal fat, peritesticular fat, subcutaneous fat, brown fat, and greater reticulum fat were significantly correlated with TC, LDL-C, and HDL-C levels; Figure 4 As shown in Figure B, the levels of 13 minerals (Mn, Zn, Ca, V, Mg, K, Fe, Co, Cu, Mo, Ba, Se, Na) were significantly correlated with GLU levels in the liver and five adipose tissues. HFCD significantly altered the concentrations of 14 minerals in the liver and five adipose tissues. Figure 3 These changes were significantly associated with elevated levels of TC, LDL-C, HDL-C, and GLU.

[0044] like Figure 5 As shown, the levels of 14 minerals (Mg, K, Mn, V, Zn, Se, Na, Ca, Cr, Fe, Co, Cu, Mo, Ba) in the liver and five adipose tissues were significantly correlated with mouse body weight. The levels of 12 minerals (Mg, K, Mn, V, Ca, Zn, Se, Na, Fe, Co, Cu, Mo) were significantly correlated with mouse fat weight. HFCD significantly altered the concentrations of these 14 minerals in the liver and five adipose tissues. Figure 3 These changes were significantly correlated with increases in body weight and fat mass.

[0045] The above results indicate that HFCD alters the content of 15 minerals in 24 tissues through four pathways: intake, absorption, excretion, and distribution, thus disrupting the body's mineral balance. Mineral imbalance is closely related to disorders of glucose and lipid metabolism, obesity, and fat accumulation; supplementing deficient minerals can help improve disorders of glucose and lipid metabolism and related diseases.

[0046] Example 2 A mineral supplement composition for weight loss through fasted exercise, comprising composition 1 and composition 2; composition 1 contains the following mineral elements: potassium, calcium, vanadium, zinc, selenium, magnesium, and cobalt; composition 2 contains the following mineral elements: manganese, copper, and iron; the mineral supplement composition is sodium-free; the content of each mineral element, based on a single daily dose, is: potassium 1000 mg, calcium 600 mg, vanadium 5 μg, manganese 3 mg, zinc 12 mg, selenium 30 μg, copper 0.5 mg, magnesium 250 mg, cobalt 0.085 μg, iron 6 mg. mg; the compound source of the mineral element potassium is potassium chloride, the compound source of the mineral element calcium is L-calcium lactate, the compound source of the mineral element vanadium is vanadium oxysulfate, the compound source of the mineral element manganese is manganese sulfate, the compound source of the mineral element zinc is zinc gluconate, the compound source of the mineral element selenite is sodium selenite, the compound source of the mineral element copper is copper sulfate, the compound source of the mineral element magnesium is magnesium citrate; the compound source of the mineral element cobalt is cyanocobalamin; and the compound source of the mineral element iron is ferrous fumarate.

[0047] Example 3 A mineral supplement composition for weight loss through fasted exercise, comprising composition 1 and composition 2; composition 1 contains the following mineral elements: potassium, calcium, vanadium, zinc, selenium, magnesium, and cobalt; composition 2 contains the following mineral elements: manganese, copper, and iron; the mineral supplement composition is sodium-free; the content of each mineral element, based on a single daily dose, is: potassium 300 mg, calcium 150 mg, vanadium 1 μg, manganese 0.5 mg, zinc 1.7 mg, selenium 3 μg, copper 0.3 mg, magnesium 53 mg, and cobalt 0.017 mg. μg, iron 2.3mg; the compound source of the mineral element potassium is potassium gluconate, the compound source of the mineral element calcium is calcium carbonate, the compound source of the mineral element vanadium is ethyl maltol vanadium oxide, the compound source of the mineral element manganese is manganese chloride, the compound source of the mineral element zinc is zinc sulfate, the compound source of the mineral element selenium is sodium selenate, the compound source of the mineral element copper is copper citrate, the compound source of the mineral element magnesium is magnesium oxide; the compound source of the mineral element cobalt is cyanocobalamin; the compound source of the mineral element iron is ferrous sulfate.

[0048] Example 4 A mineral supplement composition for weight loss through fasted exercise, comprising composition 1 and composition 2; composition 1 contains the following mineral elements: potassium, calcium, vanadium, zinc, selenium, magnesium, and cobalt; composition 2 contains the following mineral elements: manganese, copper, and iron; the mineral supplement composition is sodium-free; the content of each mineral element, based on a single daily dose, is: potassium 2000 mg, calcium 800 mg, vanadium 10 μg, manganese 2.5 mg, zinc 12 mg, selenium 40 μg, copper 1.5 mg, magnesium 330 mg, cobalt 0.17 μg, and iron 14 mg. mg; the compound source of the mineral element potassium is dipotassium hydrogen phosphate, the compound source of the mineral element calcium is calcium gluconate, the compound source of the mineral element vanadium is vanadium oxysulfate, the compound source of the mineral element manganese is manganese gluconate, the compound source of the mineral element zinc is zinc oxide, the compound source of the mineral element selenium is sodium selenate, the compound source of the mineral element copper is copper carbonate, the compound source of the mineral element magnesium is magnesium carbonate; the compound source of the mineral element cobalt is cyanocobalamin; and the compound source of the mineral element iron is ferrous gluconate.

[0049] Example 5 A mineral supplement composition powder for weight loss through fasting exercise is prepared from the mineral supplement composition obtained in Example 2 and excipients, wherein the excipients consist of 2 g of maltodextrin, 0.3 g of citric acid, 20 mg of sucralose and 50 mg of silicon dioxide.

[0050] The preparation method is as follows: weigh each raw material, pass them through an 80-mesh sieve, mix them in a three-dimensional mixer for 30 minutes, check the mixing uniformity, and package them into bags. The amounts of the mineral supplement composition and excipients obtained in Example 2 above are the content of one bag.

[0051] The method of administration is as follows: One hour after exercising on an empty stomach in the morning, dissolve one packet of powder in 250 mL of room temperature or warm water, stir well and drink.

[0052] Example 6 A mineral supplement composition specifically for women to use for weight loss through fasting exercise is presented. The difference between this composition and Example 2 is that the iron content is adjusted to 14 mg; all other aspects are the same as in Example 2. The increased iron content is due to consideration of iron loss during menstruation in women, so women trying to lose weight can appropriately increase the iron dosage.

[0053] Example 7 Animal experiments verified C57bl / 6j mice were induced with a high-fat diet for 8 weeks, followed by 4 weeks of fasting morning swimming training (30 minutes daily). Mice were randomly divided into a control group (no substances were given after exercise) and an experimental group (received the mineral supplement composition of Example 2 after exercise). A normal diet control group was also included.

[0054] The results showed that the experimental group had a 12.8% decrease in body weight (P<0.05) and a 18% decrease in adipose tissue weight compared to the control group. The levels of serum K, Ca, Zn, and Se in the experimental group after exercise did not decrease significantly compared to before exercise, while the levels of serum K, Ca, Zn, and Se in the control group after exercise decreased significantly compared to before exercise. The levels of Mg, Cu, and Mn in the liver of the experimental group returned to the levels of the control group with a normal diet.

[0055] Example 8 Human trials Thirty participants (BMI ≥ 28, 40 minutes of exercise per day) who exercised on an empty stomach in the morning were recruited and randomly divided into two groups (n=15): one group drank the mineral supplement powder of Example 5 of this invention after exercise (experimental group) and the other group drank an equal amount of placebo (the placebo here refers to water, control group) for 8 weeks.

[0056] The results showed that the average weight loss in the experimental group was 4.1±1.2 kg, while the average weight loss in the control group was 2.3±0.9 kg (P<0.05). After exercise, the serum levels of K, Zn, and Se in the experimental group were significantly higher than those in the control group, and the fatigue score was 30% lower in the experimental group than in the control group.

[0057] Analysis of the above-mentioned population trial data revealed that the increase in serum K, Zn, and Se levels after exercise was positively correlated with weight loss (r=0.58, 0.61, 0.55, P<0.05), confirming that replenishing these lost minerals is crucial for weight loss.

[0058] Example 9 A method for weight loss through exercise on an empty stomach includes the following steps: One hour after completing fasted exercise each morning, take the mineral supplement composition of Example 2; the fasted exercise lasts for ≥30 minutes and the heart rate reaches ≥60% of the maximum human heart rate. Simultaneously, control daily dietary sodium intake to <2000 mg / day; continue for at least 8 weeks.

[0059] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A mineral supplement composition for weight loss through fasted exercise, characterized in that, The composition contains composition 1 and composition 2; composition 1 contains the following mineral elements: potassium, calcium, vanadium, zinc, selenium, magnesium and cobalt; composition 2 contains the following mineral elements: manganese, copper and iron; the mineral supplement composition is sodium-free.

2. The mineral supplement composition according to claim 1, characterized in that, Based on a single daily dose, the content of each mineral element is as follows: potassium 200~2100 mg, calcium 120~900 mg, vanadium 0.5~12 μg, manganese 0.3~2.8 mg, zinc 1.5~14 mg, selenium 2~42 μg, copper 0.2~1.8 mg, magnesium 50~340 mg, cobalt 0.015~0.19 μg, and iron 2.1~16 mg.

3. The mineral supplement composition according to claim 1, characterized in that, The sources of potassium compounds include potassium gluconate, potassium citrate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, or potassium chloride; the sources of calcium compounds include calcium carbonate, calcium gluconate, calcium citrate, L-calcium lactate, calcium hydrogen phosphate, calcium chloride, calcium phosphate, glycerol calcium phosphate, calcium oxide, or calcium sulfate; the sources of vanadium compounds include vanadium oxysulfate or ethyl maltol oxyvanadium; the sources of manganese compounds include manganese sulfate, manganese chloride, manganese carbonate, manganese citrate, or manganese gluconate; and the sources of zinc compounds include zinc sulfate and zinc gluconate. Sources of mineral elements include: zinc oxide, zinc lactate, zinc citrate, zinc chloride, or zinc acetate; sources of mineral elements include: sodium selenate or sodium selenite; sources of mineral elements include: copper sulfate, copper gluconate, copper citrate, or copper carbonate; sources of mineral elements include: magnesium sulfate, magnesium chloride, magnesium oxide, magnesium carbonate, magnesium hydrogen phosphate, magnesium citrate, or magnesium gluconate; sources of mineral elements include: cyanocobalamin; sources of mineral elements include: ferrous sulfate, ferrous gluconate, ferric ammonium citrate, ferrous fumarate, ferric citrate, or ferric pyrophosphate.

4. The mineral supplement composition according to claim 1, characterized in that, Take at least 1 hour after exercising on an empty stomach. Take Composition 1 and Composition 2 separately.

5. The mineral supplement composition according to claim 4, characterized in that, The fasting exercise is defined as exercise lasting ≥30 minutes and with a heart rate reaching ≥60% of the maximum human heart rate.

6. The mineral supplement composition according to claim 1, characterized in that, The dosage forms of the mineral supplement composition include powder, effervescent tablets, oral liquid, or chewable tablets.

7. The use of the mineral supplement composition according to any one of claims 1 to 3 in the preparation of products that assist in weight loss through exercise, replenish minerals lost during exercise, and / or correct mineral deficiencies caused by a high-fat diet.

8. A method for assisting weight loss through fasted exercise, characterized in that, Includes the following steps: Take the mineral supplement composition according to any one of claims 1 to 3 at least 1 hour after completing fasted exercise each day, with composition 1 and composition 2 taken separately.

9. The method according to claim 8, characterized in that, The duration of the fasting exercise is ≥30 minutes and the heart rate reaches more than 60% of the maximum human heart rate.

10. The method according to claim 8, characterized in that, At the same time, control your daily dietary sodium intake to <2000 mg / day; continue for at least 8 weeks.

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