Endogenous hair follicle blackening hair growth essence and a preparation method thereof

Abstract

The application discloses an endogenous hair follicle blackening hair growth essence and a preparation method thereof, relates to the field of daily chemical products with hair growth and hair care effects, and is prepared by mixing active ingredients, anti-inflammatory agents and stabilizers. The prepared hair growth essence can promote the rapid and healthy growth of new hair, prevent the aging of a scalp and the premature whitening of hair, can inhibit oxidation and related enzyme activity, relieve scalp irritation and sensitivity, improve scalp inflammation, relieve hair loss, enhance or restore hair volume and original blackness, increase hair thickness, improve the health conditions of hair and a scalp, enhance scalp blood circulation, improve immunity, inhibit inflammation and resist infection so as to fully nourish hair follicles from roots, help hair growth and regeneration. In addition, the hair growth essence has good compatibility, temperature resistance and storage stability, has a high transdermal absorption rate for a scalp, and has good hair growth and hair development effects.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products with hair growth and hair care effects, A61P17 / 14, and particularly to an endogenous hair follicle blackening hair growth essence and its preparation method. Background Technology

[0002] Human hair follicles are complex structures comprising the outer root sheath, inner root sheath, hair shaft, ridge, and sebaceous gland. The hair bulb, the swollen base of the follicle, transports nutrients to it and is crucial for hair development and growth. After forming during embryonic development, hair follicles develop in a cyclical pattern. The hair development cycle consists of three phases: anagen (growth phase), catagen (transitional phase), and telogen (resting phase). Hair follicles cycle independently, allowing hair to grow, rest, and fall out simultaneously. Telogen effluvium is primarily caused by significant stress, prompting premature development of catagen and telogen follicles and premature termination of anagen follicles. Diffuse hair loss can be caused by physical or emotional stress, hormonal imbalances, dietary deficiencies, and in some cases, environmental factors and genetic predisposition. For generations, humans have suffered from thinning and falling hair. Currently, FDA-approved hair loss treatments consist of two pharmacological compounds—topical minoxidil and oral finasteride—that stimulate existing hair follicles to produce thicker hair while preventing thinning. However, both of these therapies require long-term medication use, and any benefits gained will be lost if treatment is stopped. Furthermore, adverse effects include weight gain, edema, birth defects in pregnant women, increased heart rate, angina, dermatitis, itchy erythema and dry skin, decreased libido, erectile dysfunction and other skin conditions, and even hair loss after withdrawal.

[0003] Chinese patent application CN201811502797.X discloses a traditional Chinese medicine hair growth tonic containing Angelica sinensis extract and its preparation method. It includes extracts from various plants such as Angelica sinensis, Sophora flavescens, Platycladus orientalis leaves, Salvia miltiorrhiza, Ligusticum chuanxiong, ginseng, Polygonum multiflorum, Gastrodia elata, and mulberry. These extracts work synergistically to improve scalp microcirculation, enhance hair follicle cell activity, strengthen hair roots, and promote hair growth. Chinese patent application CN201410477459.0 discloses a hair growth tonic and its preparation method, including Ginkgo biloba extract, Polygonum multiflorum extract, Panax notoginseng extract, Astragalus membranaceus extract, Cynomorium songaricum extract, Sophora flavescens extract, Ligustrum lucidum extract, peppermint extract, and ginger extract. These extracts can alter scalp blood circulation, activate hair follicles, and quickly stop hair loss and repair hair. However, these hair growth tonics cannot reduce the number of gray hairs or restore hair color, and their absorption efficiency of active ingredients is low. Summary of the Invention

[0004] To address the aforementioned problems, the first aspect of this invention provides an endogenous hair follicle blackening and hair growth essence, comprising: by weight percentage, 0.5-14% active composition, 0.07-0.2% antioxidant, 0.1-0.25% anti-inflammatory agent, 3-15% stabilizer, and water to make up the balance.

[0005] The active composition comprises: 12-20 parts by weight of plant extract, 0.6-2.9 parts by weight of enzyme inhibitor, 1.5-4.5 parts by weight of protein peptide, 0.8-2.5 parts by weight of amino acid, 0.05-0.5 parts by weight of vitamin, and 4-12 parts by weight of functional additives.

[0006] The plant extracts are selected from the following: *Platycladus orientalis* leaf extract, *Trifolium pratente* L. extract, *Linum usitatissmum* L. extract, *Artemisia argyi* Levl. Et Vant. extract, *Platycodon grandiflorus* leaf extract, *Curcuma longa* L. extract, *Alpinia officinarum* Hance extract, *Oryza sativa* Linn. subsp. japonica Kato extract, *Medicago sativa* extract, *Artemisia japonica* extract, *Camellia sinensis* leaf extract, *Ginkgobiloba* L. leaf extract, *Sargassum pallidum* (Turn.) C. Ag. extract, *Astragalus membranaceus* (Fisch.) Bge. extract, and *Aloe barbadensis* extract. At least one of the following plant extracts: (Mill) extract; preferably, the plant extract is selected from at least one of the following: Platycladus orientalis leaf extract, red clover extract, flaxseed extract, Artemisia annua extract, Platycodon grandiflorus leaf extract, turmeric extract, japonica rice extract, alfalfa extract, Artemisia japonica extract, and Sinica sylvestris leaf extract.

[0007] More preferably, the plant extract is arborvitae leaf extract, red clover extract, flaxseed extract, artemisia annua extract, turmeric extract, japonica turmeric extract, and ginkgo leaf extract.

[0008] The weight ratio of the arborvitae leaf extract, red clover extract, flaxseed extract, artemisia annua extract, turmeric extract, japonica extract, and ginkgo leaf extract is (0.3-2):(1.5-9):(2-7):(5.5-9):(1.7-8.2):(1.5-4.5):(0.5-1.5); preferably (0.25-1.8):(2.0-8):(2.5-6):(6.0-8.0):(2.5-8.0):(1.8-4.2):(0.8-1.3); more preferably 0.25:2.0:2.5:6:2.0:1.8:0.95.

[0009] More preferably, the plant extract is an extract of Oriental bellflower leaf, red clover, flaxseed, artemisia annua, turmeric, alfalfa, and ginkgo leaf.

[0010] More preferably, the plant extract is an extract of Oriental bellflower leaf, red clover, flaxseed, artemisia annua, wormwood, and ginkgo leaf.

[0011] More preferably, the plant extract is an extract of Scots leaf, a extract of red clover, an extract of flaxseed, an extract of artemisia annua, and an extract of ginkgo leaf.

[0012] Platycladus orientalis is rich in phytochemicals, such as cyclopropene (a sesquiterpene), sederoyl (a sesquiterpene alcohol), pinene, sabine (a monoterpene), and pinene diterpenes (a limonene diterpene), which exhibit therapeutic effects through various mechanisms of preventing hair loss. The Platycladus orientalis leaf extract used in this invention has strong 5α-reductase inhibitory activity and induces β-catenin and Shh protein, and can also stimulate scalp angiogenesis, exhibiting excellent anti-inflammatory effects and promoting hair growth.

[0013] Artemisia annua, rich in ethyl acetate, contains coumarin flavonoids and jasmine glycosides, which can effectively promote the cell activity of dermal and hair follicle cells, thereby inducing the production of free radicals or reactive oxygen species. Furthermore, the active ingredients in its extract can inhibit the activity of cyclooxygenase and matrix metalloproteinases in dermal cells, thus preventing their harmful effects on hair loss and scalp deterioration. It can also inhibit hair loss-related T-cell-mediated contact dermatitis, improving scalp inflammation and promoting healthy hair growth.

[0014] Flaxseed extract is rich in Omega-3, selenium, arginine, glutamic acid, aspartic acid, and other essential amino acids. It can increase hair regeneration and strengthen hair roots, while also promoting hair fiber alignment, making hair smoother, reducing scalp allergies and dandruff, significantly improving scalp health, and increasing hair length and thickness. Furthermore, its abundant fatty acids, selenium, and protein nourish hair follicles, further promoting healthy and rapid hair growth.

[0015] This application combines multiple plant extracts, among which various active ingredients exert multiple hair growth mechanisms and work synergistically to better improve scalp condition, provide more nutrients for hair growth, nourish hair follicles, and promote faster and better hair growth. Furthermore, various medicinal plant extracts and their fractions possess excellent free radical scavenging activity and excellent inhibitory activity against lipid peroxidation. Working synergistically with specific antioxidants, anti-inflammatory agents, and other components, they can help prevent or treat hair loss caused by oxidative stress, further protect hair follicles from damage caused by oxidation and inflammation, halt the hair loss cycle, induce a longer hair growth phase, promote melanin synthesis and DNA oxidation, repair gray hair, and improve perimenopausal or menopausal symptoms in elderly women.

[0016] The enzyme inhibitor is selected from at least one of azelaic acid, tetrahydropiperidine derivatives, ketoconazole, green tea extract, caffeine, and pumpkin seed oil; preferably, tetrahydropiperidine derivatives.

[0017] The protein peptide is selected from at least one of palmitoyl tripeptide, pea peptide, myristoyl pentapeptide, cell-stimulating peptide, yeast polypeptide, germination peptide, glutathione, and elastin polypeptide; preferably pea peptide, cell-stimulating peptide, and germination peptide.

[0018] The weight ratio of the pea peptide, cell-stimulating peptide, and germination peptide is (0.15-2.5):(0.10-0.25):(0.15-3.0); preferably (0.17-2.0):(0.13-0.20):(0.18-2.16); and more preferably 1.85:0.15:1.60.

[0019] The amino acid is selected from at least one of tyrosine, lysine, L-arginine, cysteine, serine, trimethylglycine, cysteine, histidine, and methionine; preferably L-arginine.

[0020] The vitamin is selected from one or more of vitamins A, B, C, and E; preferably vitamin B; and more preferably biotin.

[0021] The main component of hair is keratin. Adding vitamins can promote the synthesis of keratin to a greater extent, thereby increasing the elasticity and shine of the hair. In particular, choosing biotin can produce an enzyme that helps participate in the normal metabolism of fats, keratin, and sugars, improves blood circulation at the hair follicle roots, activates stagnant blood components to promote blood circulation at the roots, nourishes hair follicles, promotes hair growth, promotes sufficient supply of ATP and the transport and excretion of metabolites, improves scalp health, is beneficial to cell health and regeneration, maintains normal hair growth, and even increases hair coverage and thickness, improves gray hair in teenagers, and has the effects of hair growth and hair loss prevention.

[0022] The functional additive is selected from at least one of carboxymethyl chitosan, sodium hyaluronate, hydroxyethyl starch, γ-polyglutamic acid, xanthan gum, and monosodium glutamate; preferably carboxymethyl chitosan.

[0023] The antioxidant is selected from at least one of citric acid, tartaric acid, polyphenols, lutein, retina, ascorbic acid, ginger (Zingiber officinale) root extract, lycopene, ginseng (Panax Ginseng CAMey.) extract, angelica (Angelic (Sinensis (Oilv) Diels) extract, arborvitae (Platycladus orientalis (L.) Franco) leaf extract, ganoderma lucidum (Curtis) P. Karst. extract, Polygonum multiflorum Thunb root extract, 4-didehydroretina, and carotene; preferably lutein and carotene.

[0024] The carotene is selected from at least one of γ-carotene, δ-carotene, α-carotene, and β-carotene; preferably β-carotene.

[0025] The weight percentage of lutein and carotene is 1:(0.4-1.3); preferably 1:0.75.

[0026] The anti-inflammatory agent is selected from at least one of bisabolol, allantoin, dipotassium glycyrrhizate, glycyrrhizin, resveratrol, tea tree oil, menthol, Centella asiatica (L.) Urban extract, and Gentiana scabra Bunge extract; preferably dipotassium glycyrrhizate and tea tree oil.

[0027] The weight ratio of dipotassium oxalate to tea tree oil is 1:(1.2-2.3); preferably 1:1.6.

[0028] The stabilizer is selected from at least one of hydrolyzed jojoba esters, alkanolamine compounds, lauryl compounds, and stearyl (dihydroxypropyl) dimethylammonium oligosaccharides; preferably alkanolamine compounds and lauryl compounds; more preferably cocoyl diethanolamine and dioctyl dodecyl lauroyl glutamate.

[0029] The weight ratio of the cocoyl diethanolamine and dioctyl dodecyl lauroyl glutamate is 1:(0.2-1); preferably 1:0.4.

[0030] The active ingredients in this invention primarily exert their hair growth effect through plant extracts and other active ingredients. However, plant extracts have complex compositions and poor compatibility in the system, and the combined use of active ingredients can easily lead to inactivation. The applicant unexpectedly discovered that adding stabilizers, especially cocoyl diethanolamine and octyl dodecyl lauroyl glutamate, to the above system can effectively promote the compatibility between substances in the system and maintain their respective activities well, increasing the stability of the resulting hair growth essence. This may be because cocoyl diethanolamine and octyl dodecyl lauroyl glutamate contain long carbon chains, which can reduce the surface tension of substances in the system, increase the wetting and dispersibility of each substance in the system, and enable synergistic effects among the components, increasing the transdermal absorption efficiency of the active ingredients and achieving better hair growth effects. Simultaneously, they have good foam-stabilizing effects, improving the temperature resistance of the prepared composition, thereby increasing the stability of the system and allowing the resulting hair growth essence to be stored for a long time.

[0031] The polar solvent with a boiling point of 30~300℃ is selected from at least one of ethanol, methanol, 1,2-propanediol, 1,3-propanediol, glycerol, butanediol, methyl acetate, and ethyl acetate; preferably glycerol.

[0032] The glycerol is selected from at least one of synthetic glycerol, refined glycerol, hydrolyzed glycerol, and saponified glycerol; preferably hydrolyzed glycerol.

[0033] The surface tension of the hydrolyzed 1,2,3-propanetriol is 50~80 mN / m, and the viscosity at 20°C is 1200~1600 mPa•s; preferably, the surface tension is 63.3 mN / m, and the viscosity at 20°C is 1412 mPa•s.

[0034] The second aspect of this invention provides a method for preparing an endogenous hair follicle-modifying hair growth essence, comprising: S1. Add a polar solvent, antioxidant, anti-inflammatory agent, and stabilizer with a boiling point of 30~300℃ to water and stir to mix; S2. Continue to add the active composition to S1, stir and mix to obtain the final product.

[0035] In some preferred embodiments, Compared with the prior art, the present invention has the following beneficial effects: (1) The hair growth essence prepared by mixing the active ingredients with anti-inflammatory agents and stabilizers can promote the rapid and healthy growth of new hair, prevent scalp aging and premature graying of hair, and can soothe scalp irritation and sensitivity, improve scalp inflammation, relieve hair loss, enhance or restore hair volume and original blackness, increase hair thickness, improve the health of hair and scalp, enhance scalp blood circulation, improve immunity, inhibit inflammation and resist infection so as to fully nourish hair follicles from the root and help hair new growth and regeneration.

[0036] (2) The hair growth essence in this invention can effectively isolate ultraviolet rays, inhibit the oxidative damage of the hair cuticle, improve the scalp moisture and strong barrier function, prevent scalp peeling and irritation, and enhance the colony formation efficiency of epidermal stem cells and dermis, nourish hair follicles, promote the regeneration of new hair follicles, restore the vitality of hair follicles, grow new hair quickly and restore color, reduce the production of gray hair, and is fragrance-free, natural, safe and non-irritating.

[0037] (3) The hair growth essence in this invention can stimulate stem cells to activate and differentiate into new dermal and hair follicle cells, thereby initiating the growth of new hair in the scalp hair follicles. In addition, the hair growth essence has good compatibility and temperature stability, can be stored for a long time, has a high transdermal absorption rate of the scalp, has a good hair growth effect, and will not cause further hair loss or adverse reactions or withdrawal symptoms. The scalp health is faster than that of 5% minoxidil, making it a new preparation and ideal choice for treating different types of hair loss. Detailed Implementation

[0038] Example 1 1. An endogenous hair follicle blackening and hair growth essence, comprising: by weight percentage, 8.5% active composition, 0.13% antioxidant, 0.15% anti-inflammatory agent, 6.8% stabilizer, and water to make up the balance.

[0039] The active composition comprises: 15.5 parts by weight of plant extract, 1.25 parts by weight of enzyme inhibitor, 3.6 parts by weight of protein peptide, 1.6 parts by weight of amino acid, 0.25 parts by weight of vitamin, and 8 parts by weight of functional additives.

[0040] The plant extracts are arborvitae leaf extract, red clover extract, flaxseed extract, artemisia annua extract, turmeric extract, japonica turmeric extract, and ginkgo leaf extract.

[0041] The weight ratio of the arborvitae leaf extract, red clover extract, flaxseed extract, artemisia annua extract, turmeric extract, japonica extract, and ginkgo leaf extract is 0.25:2.0:2.5:6:2.0:1.8:0.95.

[0042] Red clover extract was purchased from Shaanxi Sinote Biotechnology Co., Ltd., flaxseed extract from Shaanxi Xintianyu Biotechnology Co., Ltd., artemisia annua extract from Shaanxi Haisai Biotechnology Co., Ltd., turmeric extract from Shandong Huiheng Biotechnology Co., Ltd., and ginkgo leaf extract from Shaanxi Haisai Biotechnology Co., Ltd.

[0043] The enzyme inhibitor is a tetrahydropiperidine derivative.

[0044] The protein peptides are pea peptides, cell-stimulating peptides, and germination peptides.

[0045] The weight ratio of the pea peptide, cell-stimulating peptide, and germination peptide is 1.85:0.15:1.60.

[0046] The pea peptides were purchased from Shaanxi Yuanyou Biotechnology Co., Ltd., and the CAS number of the sprouting peptides is 299157-54-3.

[0047] The amino acid in question is L-arginine.

[0048] The vitamin in question is a B vitamin; the B vitamin in question is biotin.

[0049] The functional additive is carboxymethyl chitosan.

[0050] The antioxidants are lutein and carotene.

[0051] The carotene mentioned is β-carotene.

[0052] The weight percentage of lutein and carotene is 1:0.75.

[0053] The anti-inflammatory agents are dipotassium glycyrrhizate and tea tree oil (purchased from Jiangxi Hengcheng Natural Fragrance Oil Co., Ltd.).

[0054] The weight ratio of dipotassium oxalate to tea tree oil is 1:1.6.

[0055] The stabilizers are alkanolamine compounds and lauryl compounds; specifically, cocoyl diethanolamine (CAS No.: 68603-42-9) and dioctyl dodecyl lauroyl glutamate.

[0056] The weight ratio of the cocoyl diethanolamine to dioctyl dodecyl lauroyl glutamate is 1:0.4.

[0057] The polar solvent with a boiling point of 30~300℃ is hydrolyzed glycerol.

[0058] The surface tension of the hydrolyzed glycerol is 63.3 mN / m, and the viscosity at 20°C is 1412 mPa·s (Green Treasure Indonesia, purchased from Guangzhou Yehusheng Chemical Co., Ltd.).

[0059] 2. A method for preparing an endogenous hair follicle-regenerating hair growth essence, comprising: S1. Add a polar solvent, antioxidant, anti-inflammatory agent, and stabilizer with a boiling point of 30~300℃ to water and stir to mix; S2. Continue to add the active composition to S1, stir and mix to obtain the final product.

[0060] Example 2 1. An endogenous hair follicle darkening and hair growth essence, which differs from Example 1 in that: The plant extracts are Oriental bellflower leaf extract, red clover extract, flaxseed extract, artemisia annua extract, turmeric extract, alfalfa extract, and ginkgo leaf extract.

[0061] The weight ratio of the arborvitae leaf extract, red clover extract, flaxseed extract, artemisia annua extract, turmeric extract, alfalfa extract, and ginkgo leaf extract is 0.25:2.0:2.5:6:2.0:1.8:0.95.

[0062] The alfalfa extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0063] 2. A method for preparing an endogenous hair follicle blackening and hair growth essence, the same as in Example 1.

[0064] Example 3 1. An endogenous hair follicle darkening and hair growth essence, which differs from Example 1 in that: The plant extracts are Oriental bellflower leaf extract, red clover extract, flaxseed extract, artemisia annua extract, japonica wormwood extract, and ginkgo leaf extract.

[0065] The weight ratio of the Oriental bellflower leaf extract, red clover extract, flaxseed extract, artemisia annua extract, japonica wormwood extract, and ginkgo leaf extract is 0.25:2.0:2.5:6:1.8:0.95.

[0066] 2. A method for preparing an endogenous hair follicle blackening and hair growth essence, the same as in Example 1.

[0067] Example 4 1. An endogenous hair follicle darkening and hair growth essence, which differs from Example 1 in that: The plant extracts are: Oriental camphor leaf extract, red clover extract, flaxseed extract, artemisia annua extract, and ginkgo leaf extract.

[0068] The weight ratio of the extracts of Camphor Tree Leaf, Red Clover, Flaxseed, Artemisia annua, and Ginkgo Leaf is 1.8:2.0:2.5:6:0.95.

[0069] 2. A method for preparing an endogenous hair follicle blackening and hair growth essence, the same as in Example 1.

[0070] Example 5 1. An endogenous hair follicle darkening and hair growth essence, which differs from Example 1 in that: The weight ratio of the cocoyl diethanolamine to dioctyl dodecyl lauroyl glutamate is 1:2.

[0071] 2. A method for preparing an endogenous hair follicle blackening and hair growth essence, the same as in Example 1.

[0072] Example 6 1. An endogenous hair follicle darkening and hair growth essence, which differs from Example 1 in that: The glycerin is refined glycerin (purchased from Shanghai Shengyue International Trade Co., Ltd.).

[0073] 2. A method for preparing an endogenous hair follicle blackening and hair growth essence, the same as in Example 1.

[0074] Performance testing 1. Physicochemical performance testing: Based on the technical requirements and our laboratory's GDYJJC-SOP-01-031 "Declaration of Conformity, Rules and Operating Procedures", a simple acceptance (risk-sharing) judgment rule was adopted. Specific test results are shown in Table 1.

[0075] Table 1 Performance test results of the sample obtained in Example 1

[0076] 2. Stability Test: In accordance with the technical requirements and our laboratory's GDYJJC-SOP-01-031, the temperature stability of the example and the obtained hair growth essence was tested. The test results are shown in Table 2.

[0077] The acceptable result is: after returning to room temperature, there is no significant difference in appearance compared to before the test; The following are considered unacceptable: After returning to room temperature, the product exhibits significant differences in properties compared to its pre-test state.

[0078] 3. Efficacy Test: (1) Clinical trials of the samples obtained in Example 1 on hair growth in patients with different types of hair loss.

[0079] A. A 41-year-old male used the hair growth essence of the present invention as described in Example 4, applied topically twice a day, for 4 months.

[0080] Four months later, users reported improvements in scalp and dandruff, increased hair growth and volume, and a healthier scalp.

[0081] B. A 57-year-old female patient used the hair growth essence of the present invention as described in Example 4, applied it twice a day, and examined it by hair drop microscopy.

[0082] The significant increase in new hair follicles and new tips, which are stronger and thicker, increases the coverage of the affected area of ​​the scalp by increasing the amount of hair.

[0083] (2) Effects on the expression of hair-plucking genes: This study aimed to investigate the effects of different percentages of the ingredients in four different formulations of the hair growth serum of this invention on gene expression in hair follicles. Hair growth serums with different concentrations of the ingredients were formulated and labeled A, B, C, and D, and applied to 10 subjects over two weeks, morning and evening. Twenty follicle pools were harvested before and after treatment, sufficient RNA was extracted for quantitative gene expression profiling. Thirty genes important for hair physiology were subsequently tracked. In addition to regulating the expression of genes involved in hair pigmentation, the results showed that the expression of fibroblast growth factor 7 (FGF7) and noggin was primarily regulated.

[0084] Formula A is a combination of all the functional components that strongly activates both genes. After the second week of treatment, FGF7 expression increased by an average of 62%, and noggin expression increased by an average of 86%. FGF7 was stimulated in 8 out of 10 participants.

[0085] The expression products of genes FGF7 and noggin, FGF7 and noggin proteins, are well-known signaling compounds important for inducing new hair growth phases. These two proteins are primarily synthesized in dermal cells. The hair follicle used for gene expression analysis comprises the entire hair bulb surrounding the dermis. Therefore, the extracted RNA originated not only from matrix keratinocytes but also from adjacent mesenchymal cells. The gene expression profiles before and after treatment indicate that the hair growth essence obtained in Example 3 of this invention stimulates hair growth, promotes hair color restoration, and has anti-aging effects on the scalp.

[0086] (3) Hair regrowth effect: This study involved 18 participants (11 men and 7 women), with a mean age of 56 years (range 38 to 72 years). Each participant was treated daily by topical application of the hair growth serum product derived in Example 3 to the affected area of ​​the scalp. Peer-controlled areas were not considered controls. The appearance of new and regrowth hair on the patients' scalps was systematically assessed using trichomoniasis.

[0087] Data for each participant was recorded before, during, and after treatment, and differences between control and treatment areas were determined using a special camera with photo-stereoscopic images.

[0088] Hair length: Regular examinations using optical glasses revealed that the length of hair on the scalp increased as the hair color returned to normal.

[0089] Test results: The photosensitive pen examination showed significant differences in hair length and thickness, ranging from approximately 18% to as high as 74%.

[0090] Hair count: The image below, obtained by treating individual participants' scalps with a light pen, shows that the number of new terminal hairs increases with the increase of hair follicle and hair thickness.

[0091] In the control scalp with hair, the increased number of hairs on the treated scalp resulted in stronger, thicker hairs in the treated area, and a significant increase in the number of hairs covering a larger area of ​​the scalp.

Claims

1. An endogenous hair follicle-regenerating hair growth essence, characterized in that, It includes: By weight percentage, 0.5-14% active composition, 0.07-0.2% antioxidant, 0.1-0.25% anti-inflammatory agent, 3-15% stabilizer, water to make up the balance.

2. The endogenous hair follicle darkening and hair regrowth essence according to claim 1, characterized in that, The active composition comprises: 12-20 parts by weight of plant extract, 0.6-2.9 parts by weight of enzyme inhibitor, 1.5-4.5 parts by weight of protein peptide, 0.8-2.5 parts by weight of amino acid, 0.05-0.5 parts by weight of vitamin, and 4-12 parts by weight of functional additives.

3. The endogenous hair follicle darkening and hair regrowth essence according to claim 2, characterized in that, The plant extract is selected from at least one of the following: Platycladus orientalis leaf extract, red clover extract, flaxseed extract, Artemisia annua extract, Platycodon grandiflorus leaf extract, turmeric extract, Alpinia galanga extract, japonica rice extract, alfalfa extract, Artemisia japonica extract, Sinica sylvestris leaf extract, Ginkgo biloba leaf extract, seaweed extract, Astragalus membranaceus extract, and aloe vera extract.

4. The endogenous hair follicle darkening and hair regrowth essence according to claim 2 or 3, characterized in that, The plant extracts are arborvitae leaf extract, red clover extract, flaxseed extract, artemisia annua extract, turmeric extract, japonica turmeric extract, and ginkgo leaf extract; The weight ratio of the arborvitae leaf extract, red clover extract, flaxseed extract, artemisia annua extract, turmeric extract, japonica extract and ginkgo leaf extract is (0.3-2):(1.5-9):(2-7):(5.5-9):(1.7-8.2):(1.5-4.5):(0.5-1.5).

5. The endogenous hair follicle darkening and hair regrowth essence according to claim 4, characterized in that, The enzyme inhibitor is selected from at least one of azelaic acid, tetrahydropiperidine derivatives, ketoconazole, green tea extract, caffeine, and pumpkin seed oil.

6. The endogenous hair follicle darkening and hair regrowth essence according to claim 5, characterized in that, The protein peptide is selected from at least one of palmitoyl tripeptide, pea peptide, myristoyl pentapeptide, cell-stimulating peptide, yeast polypeptide, germination peptide, glutathione, and elastin polypeptide.

7. The endogenous hair follicle darkening and hair regrowth essence according to claim 6, characterized in that, The amino acid is selected from at least one of tyrosine, lysine, L-arginine-cysteine, serine, trimethylglycine, cysteine, histidine, and methionine.

8. The endogenous hair follicle darkening and hair regrowth essence according to claim 7, characterized in that, The antioxidant is selected from at least one of citric acid, tartaric acid, polyphenols, lutein, retina, ascorbic acid, ginger root extract, lycopene, ginseng extract, angelica extract, arborvitae leaf extract, ganoderma extract, polygonum multiflorum root extract, 4-didehydroretina, and carotene.

9. The endogenous hair follicle darkening and hair regrowth essence according to claim 8, characterized in that, The stabilizer is selected from at least one of hydrolyzed jojoba esters, alkanolamine compounds, lauryl compounds, and stearyl (dihydroxypropyl) dimethylammonium oligosaccharides.

10. A method for preparing an endogenous hair follicle-regenerating hair growth essence according to any one of claims 1-9, characterized in that, include: S1. Add a polar solvent, antioxidant, anti-inflammatory agent, and stabilizer with a boiling point of 30~300℃ to water and stir to mix; S2. Continue to add the active composition to S1, stir and mix to obtain the final product.

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