A composition containing recombinant collagen and its use in a lyophilized mask

Abstract

The application belongs to the technical field of cosmetic preparation, and particularly relates to a composition containing recombinant collagen and application of the composition in preparation of a freeze-dried mask. The composition is prepared by mixing recombinant collagen, acrylic acid (ester) copolymer sodium, calcium pantothenate, sodium hyaluronate, panthenol, yellow collagen, polyglycerol-10 oleate, glycerol, jojoba seed oil, lecithin, tocopherol, palmitoyl pentapeptide-4, pinoquercetin, licorice extract, centella asiatica extract, tremella extract and water, and each component plays a synergistic effect, can effectively promote recovery of skin barrier structure, repair damage, strengthen skin barrier and resist oxidative damage. Further, the composition is used to prepare a freeze-dried mask, and the freeze-dried mask is dehydrated by freeze-drying, retains polypeptide, flavone and plant extract activity, and after reconstitution, can replenish water and lipids, relieve and repair, reduce irritation, and is suitable for sensitive and barrier-damaged skin.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic preparation technology, specifically relating to a composition containing recombinant collagen and its application in the preparation of freeze-dried facial masks. Background Technology

[0002] Modern people commonly face multiple skin problems, such as irregular sleep patterns due to staying up late, over-cleansing, and frequent chemical peels and cosmetic procedures. These, combined with external stimuli like UV rays and seasonal temperature fluctuations, make skin barrier damage a core skin concern. Loss of lipids in the stratum corneum reduces the skin's ability to retain moisture, often accompanied by redness, dryness, itching, stinging, burning, and increased sensitivity. Meanwhile, conventional liquid mask systems have limitations. A large number of active repairing ingredients, when soaked in aqueous solutions for extended periods, are easily degraded and deactivated by oxygen and temperature. To inhibit microbial growth, formulas must include preservatives, thickeners, emulsifiers, and other excipients. These substances can potentially irritate fragile skin, easily triggering breakouts and allergies, making it difficult to simultaneously meet the need for high activity and low irritation. Freeze-dried masks thus emerged to address this need.

[0003] Freeze-dried facial masks utilize a vacuum freeze-drying process. A mask sheet soaked in an active ingredient solution is frozen at low temperatures, and the water is removed directly through sublimation in a vacuum environment, resulting in a solid, dried mask. The primary goal in preparing freeze-dried masks is to avoid the damage to active ingredients caused by aqueous solutions, maximizing the preservation of the bioactivity of repairing components such as peptides, polysaccharides, and recombinant collagen without high-temperature treatment. Because the finished product contains almost no water, microorganisms cannot survive and reproduce, eliminating the need for traditional preservatives, significantly reducing irritation, and simplifying the formula by reducing redundant excipients such as thickeners. However, current freeze-dried masks have a weakness in terms of weak occlusive moisturizing ability. Summary of the Invention

[0004] The purpose of this invention is to provide a composition containing recombinant collagen and its application in the preparation of freeze-dried facial masks. The resulting freeze-dried facial masks have strong moisturizing ability and good whitening and antioxidant effects.

[0005] This invention provides a composition containing recombinant collagen, comprising, by weight parts: 1-3 parts recombinant collagen, 2-4 parts sodium acrylate copolymer, 0.1-0.5 parts calcium pantothenate, 0.5-1 parts sodium hyaluronate, 0.1-0.3 parts panthenol, 0.1-0.3 parts xanthan collagen, 3-5 parts polyglycerol-10 oleate, 2-5 parts glycerin, 5-8 parts jojoba seed oil, 0.5-3 parts lecithin, 0.1-0.5 parts tocopherol, 1.3-1.8 parts palmitoyl pentapeptide-4, 0.1-0.3 parts quinacrine, 1-3 parts licorice root extract, 1-3 parts centella asiatica extract, 2-6 parts tremella fuciformis extract, and 50-70 parts water.

[0006] Preferably, the recombinant collagen comprises recombinant type I collagen and recombinant type III collagen, wherein the mass ratio of recombinant type I collagen to recombinant type III collagen is 1:1.5-2.

[0007] Preferably, the licorice extract is an alcohol extract obtained by extraction with a 65%-85% v / v ethanol solution; The Centella asiatica extract is an alcoholic extract obtained by extraction with a 60%-70% v / v ethanol solution.

[0008] Preferably, the preparation method of the Tremella fuciformis extract is as follows: (1) After crushing the fruiting body of Tremella fuciformis, mix it with water to obtain a mixture; (2) The mixture was mixed with Bacillus subtilis for primary fermentation to obtain the primary fermentation product; (3) Mix the primary fermentation product, brewer's yeast liquid and rice flour for secondary fermentation to obtain the secondary fermentation product; (4) The secondary fermentation products were centrifuged and filtered sequentially to obtain the supernatant; the supernatant was mixed with trehalose and then freeze-dried to obtain the tremella extract.

[0009] Preferably, in step (1), the mass-to-volume ratio of the pulverized tremella to water is 1g:40-50mL.

[0010] Preferably, in step (2), the mass ratio of the mixture to Bacillus subtilis is 100:1-2; The effective viable count of Bacillus subtilis is ≥1×10⁻⁶. 7 CFU / g; The primary fermentation temperature is 35-38℃, and the time is 24-48h.

[0011] Preferably, in step (3), the mass ratio of the primary fermentation product, the brewing yeast liquid, and the rice flour is 100:3-5:1-3; The effective viable count of the brewer's yeast culture is ≥1×10⁻⁶. 6 CFU / mL; The secondary fermentation is carried out at a temperature of 25-30℃ for 24-48 hours.

[0012] Preferably, in step (4), the volume-to-mass ratio of the supernatant to trehalose is 100 mL: 3-5 g.

[0013] This invention provides the application of the composition described in the above technical solution in the preparation of freeze-dried facial masks.

[0014] This invention provides a method for preparing a freeze-dried facial mask, comprising the following steps: The composition described in the above technical solution is mixed evenly to obtain a mask stock solution; after soaking the mask roll with the mask stock solution, the mask roll is shaped and freeze-dried to obtain a freeze-dried mask.

[0015] Beneficial effects: This invention blends recombinant collagen, sodium acrylate copolymer, calcium pantothenate, sodium hyaluronate, panthenol, yellow collagen, polyglycerol-10 oleate, glycerin, jojoba seed oil, lecithin, tocopherol, palmitoyl pentapeptide-4, flavonoids, licorice root extract, centella asiatica extract, tremella fuciformis extract, and water. The components work synergistically to repair the skin barrier. Recombinant collagen replenishes stratum corneum collagen and repairs the damaged barrier; panthenol and calcium pantothenate work synergistically to soothe inflammation and improve redness, dryness, and itching; sodium hyaluronate and glycerin enhance hydration and moisture retention. Palmitoyl pentapeptide promotes skin repair, while flavonoids and various plant extracts provide antioxidant and soothing effects, reducing external inflammatory stress. Yellow collagen and sodium acrylate copolymer act as film-forming excipients, ensuring freeze-drying and reconstitution effects; polyglycerol-10 oleate and lecithin help with oil-water compatibility; jojoba seed oil and tocopherol replenish lipids and resist oxidative damage.

[0016] Based on the above technical advantages, the present invention utilizes the above composition to prepare a freeze-dried facial mask. Relying on the advantages of freeze-drying to lock in activity, the water is removed by freeze-drying, while retaining the maximum activity of peptides, flavonoids and plant extracts. After reconstitution, it can simultaneously replenish moisture, replenish lipids, soothe and repair, reduce irritation, and is suitable for sensitive and skin with damaged barrier. Detailed Implementation

[0017] In this invention, unless otherwise specified, the reagents, equipment and methods used are all conventional selections.

[0018] Centella asiatica in Latin is Centella asiatica (L.) Urb.; The usable part is the dried whole herb; The Latin name for licorice root is... Glycyrrhiza glabra L.; the usable parts are the dried roots and rhizomes; Silver ear fungus (Tremella fuciformis) in Latin is Tremella fuciformis Berk.; The usable part is a subentity.

[0019] To further illustrate the present invention, the solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0020] Example 1 A composition containing recombinant collagen, prepared by the following method: (1) The dried whole plant of Centella asiatica was pulverized to 80 mesh to obtain Centella asiatica pulverized material; the Centella asiatica pulverized material was extracted by reflux with 60% V / V ethanol solution. The number of reflux extractions was 2, the material-liquid ratio was 1g:10mL, and the extraction time was 40min each time. The extracts were combined and filtered. The filtrate was eluted with resin, concentrated and dried to obtain Centella asiatica extract. The dried licorice root was pulverized to 80 mesh to obtain licorice powder. The licorice powder was extracted by reflux with 65% V / V ethanol solution. The reflux extraction was performed twice, with a material-to-liquid ratio of 1g:10mL, for 40min each time. The extracts were combined and filtered. The filtrate was eluted with resin, concentrated and dried to obtain licorice extract. (2) The dried fruiting body of Tremella fuciformis is pulverized to 80 mesh to obtain Tremella fuciformis powder; the Tremella fuciformis powder is mixed with water at a ratio of 1g:50mL to obtain a mixture; Bacillus subtilis ( ) is added to the mixture. Bacillus subtilis The effective viable bacteria count is 1×10⁻⁶. 7 After mixing thoroughly (CFU / g, the mass ratio of the mixture to Bacillus subtilis is 100:2), it was fermented at 35℃ for 24 hours to obtain the primary fermentation product; then, Saccharomyces cerevisiae inoculum was added to the primary fermentation product. Saccharomyces cerevisiae, The primary fermentation product, the brewing yeast culture liquid, and the rice flour (rice flour to 80 mesh) were mixed with an effective viable count of 1×10⁷ CFU / mL and rice flour (rice flour to 80 mesh). The mass ratio of the primary fermentation product, the brewing yeast culture liquid, and the rice flour was 100:3:1. After mixing the three components evenly, a secondary fermentation was carried out at 25℃ for 24 hours to obtain a secondary fermentation product. The secondary fermentation product was then centrifuged and filtered to obtain a supernatant. The supernatant was mixed with trehalose at a ratio of 100mL:5g and then freeze-dried to obtain the tremella extract.

[0021] (3) By mass, the composition comprises the following raw materials: 1 part recombinant collagen, 4 parts sodium acrylate copolymer, 0.1 part calcium pantothenate, 1 part sodium hyaluronate, 0.1 part panthenol, 0.1 part yellow collagen, 3 parts polyglycerol-10 oleate, 5 parts glycerin, 8 parts jojoba seed oil, 3 parts lecithin, 0.5 parts tocopherol, 1.8 parts palmitoyl pentapeptide-4, 0.1 part 5-hydroxyflavone, 1 part licorice root extract, 1 part centella asiatica extract, 6 parts tremella extract, and 70 parts water; wherein the mass ratio of recombinant type I collagen to recombinant type III collagen is 1:2.

[0022] Example 2 A composition containing recombinant collagen, prepared by the following method: (1) The dried whole plant of Centella asiatica was pulverized to 80 mesh to obtain Centella asiatica pulverized material; the Centella asiatica pulverized material was extracted by reflux with 70% V / V ethanol solution, the number of reflux extractions was 2, the material-liquid ratio was 1g:10mL, and the extraction time was 40min each time. The extracts were combined and filtered. The filtrate was eluted with resin, concentrated and dried to obtain Centella asiatica extract. The dried licorice root was pulverized to 80 mesh to obtain licorice powder. The licorice powder was extracted by reflux with 85% V / V ethanol solution twice, with a material-to-liquid ratio of 1g:10mL, for 40min each time. The extracts were combined and filtered. The filtrate was eluted with resin, concentrated and dried to obtain licorice extract. (2) The dried fruiting body of Tremella fuciformis is pulverized to 80 mesh to obtain Tremella fuciformis powder; the Tremella fuciformis powder is mixed with water at a ratio of 1g:40mL to obtain a mixture; Bacillus subtilis ( ) is added to the mixture. Bacillus subtilis The effective viable bacteria count is 1×10⁻⁶. 7 After mixing thoroughly (CFU / g, the mass ratio of the mixture to Bacillus subtilis is 100:2), it was fermented at 35℃ for 24 hours to obtain the primary fermentation product; then, Saccharomyces cerevisiae inoculum was added to the primary fermentation product. Saccharomyces cerevisiae, The effective viable bacteria count is 1×10 7 The mixture consisted of CFU / mL of rice flour (rice flour to 80 mesh); the mass ratio of the primary ferment, the brewing yeast culture liquid, and the rice flour was 100:5:3; after mixing the three components evenly, a secondary fermentation was carried out at 25℃ for 24 hours to obtain the secondary ferment; the secondary ferment was then centrifuged and filtered to obtain the supernatant; the supernatant was mixed with trehalose at a ratio of 100mL:5g and then freeze-dried to obtain the tremella extract.

[0023] (3) By mass, the composition comprises the following raw materials: 3 parts recombinant collagen, 2 parts sodium acrylate copolymer, 0.5 parts calcium pantothenate, 0.5 parts sodium hyaluronate, 0.3 parts panthenol, 0.3 parts yellow collagen, 5 parts polyglycerol-10 oleate, 2 parts glycerin, 5 parts jojoba seed oil, 0.5 parts lecithin, 0.1 parts tocopherol, 1.3 parts palmitoyl pentapeptide-4, 0.3 parts quinacrine, 3 parts licorice root extract, 3 parts centella asiatica extract, 2 parts tremella extract, and 70 parts water; wherein the mass ratio of recombinant type I collagen to recombinant type III collagen is 1:1.5.

[0024] Example 3 A composition containing recombinant collagen, prepared by the following method: (1) The dried whole plant of Centella asiatica was pulverized to 80 mesh to obtain Centella asiatica pulverized material; the Centella asiatica pulverized material was extracted by reflux with 65% V / V ethanol solution. The number of reflux extractions was 2, the material-liquid ratio was 1g:10mL, and the extraction time was 40min each time. The extracts were combined and filtered. The filtrate was eluted with resin, concentrated and dried to obtain Centella asiatica extract. The dried licorice root was pulverized to 80 mesh to obtain licorice powder. The licorice powder was extracted by reflux with 75% V / V ethanol solution twice, with a material-to-liquid ratio of 1g:10mL, for 40min each time. The extracts were combined and filtered. The filtrate was eluted with resin, concentrated and dried to obtain licorice extract. (2) The dried fruiting body of Tremella fuciformis is pulverized to 80 mesh to obtain Tremella fuciformis powder; the Tremella fuciformis powder is mixed with water at a ratio of 1g:50mL to obtain a mixture; Bacillus subtilis ( ) is added to the mixture. Bacillus subtilis The effective viable bacteria count is 1×10⁻⁶. 8 After mixing thoroughly (CFU / g, the mass ratio of the mixture to Bacillus subtilis is 100:2), it was fermented at 35℃ for 24 hours to obtain the primary fermentation product; then, Saccharomyces cerevisiae inoculum was added to the primary fermentation product. Saccharomyces cerevisiae, The primary fermentation product, the brewing yeast culture liquid, and the rice flour (rice flour to 80 mesh) were mixed with an effective viable count of 1×10⁷ CFU / mL and rice flour (rice flour to 80 mesh). The mass ratio of the primary fermentation product, the brewing yeast culture liquid, and the rice flour was 100:3:1. After mixing the three components evenly, a secondary fermentation was carried out at 25℃ for 24 hours to obtain a secondary fermentation product. The secondary fermentation product was then centrifuged and filtered to obtain a supernatant. The supernatant was mixed with trehalose at a ratio of 100mL:5g and then freeze-dried to obtain the tremella extract.

[0025] (3) The composition comprises the following raw materials: 2 parts of recombinant collagen, 3 parts of sodium acrylate copolymer, 0.3 parts of calcium pantothenate, 0.6 parts of sodium hyaluronate, 0.2 parts of panthenol, 0.2 parts of yellow collagen, 4 parts of polyglycerol-10 oleate, 4 parts of glycerin, 6 parts of jojoba seed oil, 2 parts of lecithin, 0.4 parts of tocopherol, 1.5 parts of palmitoyl pentapeptide-4, 0.2 parts of 5-hydroxyflavone, 2 parts of licorice root extract, 2 parts of centella asiatica extract, 4 parts of tremella extract, and 70 parts of water; wherein the mass ratio of recombinant type I collagen to recombinant type III collagen is 1:2.

[0026] Comparative Example 1 The difference from Example 1 is that (1) in Comparative Example 1 is as follows: (1) The dried whole plant of Centella asiatica was pulverized to 80 mesh to obtain Centella asiatica pulverized material; the Centella asiatica pulverized material was extracted by reflux with 80% V / V ethanol solution. The number of reflux extractions was 2, the material-liquid ratio was 1g:10mL, and the extraction time was 40min each time. The extracts were combined and filtered. The filtrate was eluted with resin, concentrated and dried to obtain Centella asiatica extract. The dried licorice root was pulverized to 80 mesh to obtain licorice powder. The licorice powder was extracted by reflux with 90% v / v ethanol solution twice, with a material-to-liquid ratio of 1g:10mL, for 40min each time. The extracts were combined and filtered. The filtrate was then eluted with resin, concentrated and dried to obtain licorice extract.

[0027] Comparative Example 2 The difference from Example 1 is that (1) in Comparative Example 2 is as follows: (1) The dried whole plant of Centella asiatica was pulverized to 80 mesh to obtain Centella asiatica pulverized material; the Centella asiatica pulverized material was extracted by boiling in water. The boiling and extraction were carried out twice, with a material-to-liquid ratio of 1g:10mL, for 40min each time. The extracts were combined, filtered, concentrated and dried to obtain Centella asiatica extract. The dried licorice root was pulverized to 80 mesh to obtain licorice powder. The licorice powder was extracted by boiling in water twice, with a material-to-liquid ratio of 1g:10mL, for 40min each time. The extracts were combined, filtered, concentrated and dried to obtain licorice extract.

[0028] Comparative Example 3 The difference from Example 1 is that (2) in Comparative Example 3 is as follows: (2) The dried fruiting body of Tremella fuciformis is pulverized to 80 mesh to obtain Tremella fuciformis powder; the Tremella fuciformis powder is mixed with water at a ratio of 1g:50mL to obtain a mixture; Bacillus subtilis ( ) is added to the mixture. Bacillus subtilis The effective viable bacteria count is 1×10⁻⁶. 8 CFU / g) and Saccharomyces cerevisiae culture ( Saccharomyces cerevisiae, The effective viable bacteria count is 1×10 7 The mixture, Bacillus subtilis and Saccharomyces cerevisiae in a mass ratio of 100:2:3 was fermented at 30°C for 48 hours to obtain the fermented product. The fermented product was centrifuged and filtered to obtain the supernatant. The supernatant was mixed with trehalose at a ratio of 100mL:5g and then freeze-dried to obtain the Tremella fuciformis extract.

[0029] Comparative Example 4 The difference from Example 1 is that (2) in Comparative Example 4 is as follows: (2) The dried fruiting body of Tremella fuciformis is pulverized to 80 mesh to obtain Tremella fuciformis powder; the Tremella fuciformis powder is mixed with water at a ratio of 1g:50mL to obtain a mixture; Bacillus subtilis ( ) is added to the mixture. Bacillus subtilis The effective viable bacteria count is 1×10⁻⁶. 7 After mixing the mixture (CFU / g, with a mass ratio of 100:2 to Bacillus subtilis) evenly, it was fermented at 35℃ for 24 hours to obtain the primary fermentation product. The primary fermentation product was centrifuged and filtered to obtain the supernatant. The supernatant was mixed with trehalose at a ratio of 100mL:5g and then freeze-dried to obtain the Tremella fuciformis extract.

[0030] Comparative Example 5 The difference from Example 1 is that (2) in Comparative Example 5 is as follows: (2) The dried fruiting body of Tremella fuciformis is pulverized to 80 mesh to obtain Tremella fuciformis powder; the Tremella fuciformis powder is mixed with water at a ratio of 1g:50mL to obtain a mixture; Bacillus subtilis ( ) is added to the mixture. Bacillus subtilis The effective viable bacteria count is 1×10⁻⁶. 7 After mixing thoroughly (CFU / g, the mass ratio of the mixture to Bacillus subtilis is 100:2), it was fermented at 35℃ for 24 hours to obtain the primary fermentation product; then, Saccharomyces cerevisiae inoculum was added to the primary fermentation product. Saccharomyces cerevisiae, The effective viable count is 1×10 7 The mixture consists of CFU / mL of rice flour (rice flour to 80 mesh); the mass ratio of the primary ferment, the brewing yeast culture liquid, and the rice flour is 100:3:1; after mixing the three components evenly, a secondary fermentation is carried out at 25℃ for 24 hours to obtain the secondary ferment; the secondary ferment is then centrifuged and filtered to obtain the supernatant; the supernatant is then freeze-dried to obtain the tremella extract.

[0031] Comparative Example 6 The difference from Example 1 is that (3) in Comparative Example 6 is as follows: (3) By mass, the composition comprises the following raw materials: 1 part of recombinant type I collagen, 4 parts of sodium acrylate copolymer, 0.1 part of calcium pantothenate, 1 part of sodium hyaluronate, 0.1 part of panthenol, 0.1 part of yellow collagen, 3 parts of polyglycerol-10 oleate, 5 parts of glycerin, 8 parts of jojoba seed oil, 3 parts of lecithin, 0.5 parts of tocopherol, 1.8 parts of palmitoyl pentapeptide-4, 0.1 part of 5-hydroxyflavone, 1 part of licorice root extract, 1 part of centella asiatica extract and 70 parts of water.

[0032] DPPH free radical scavenging verification The compositions prepared in Examples 1-3 and Comparative Examples 1-6 were dissolved in sterile water to obtain test solutions with a final concentration of 1 mg / mL. The experimental setup is as follows: i: Add 2 mL of deionized water + 2 mL of DPPH solution to the PE tube; j: Add 2 mL of the test solution + 2 mL of DPPH solution to the PE tube; k: Add 2 mL of the test solution and 2 mL of anhydrous ethanol to the PE tube; Mix the above tubes thoroughly, let stand in the dark for 30 minutes, and measure their absorbance sequentially at a wavelength of 517 nm using a UV spectrophotometer. Calculate the scavenging rate (%) = [1-(Aj-Ak) / Ai]×100%; where Ai is the absorbance of the DPPH ethanol solution; Aj is the absorbance of the test solution for scavenging free radicals; and Ak is the absorbance of the test solution. The results are shown in Table 1.

[0033] Table 1. DPPH radical scavenging rate of different test solutions

[0034] Based on the data in Table 1, the compositions prepared in both the examples and the comparative examples have good antioxidant properties. However, the compositions in the examples have a higher DPPH free radical scavenging rate, meaning their antioxidant properties are better than those in the comparative examples.

[0035] Whitening effect verification Based on the results in Table 1, the compositions prepared in Examples 1-3 and Comparative Examples 1-5 were selected for testing; the above compositions were diluted with DMEM medium to obtain test solutions with a final concentration of 0.1 g / L; mouse B16 melanoma cells were used as test cells, and after the cells grew to the confluence state, they were passaged.

[0036] Collect cells from the same passage, and when the cells reach a near-confluence state, digest them with 0.25% trypsin to adjust the cell concentration to 10. 5Cells were cultured at a concentration of 1 mL / well in a 6-well plate. After cell attachment, the supernatant was discarded, and the test solution was added to each well (five replicates of each test solution were used, and the average value was taken). A treatment with an equal volume of DMEM medium was used as a blank control. After 72 h of culture, cells were digested with 0.25% trypsin for 3 min, collected, and centrifuged. The supernatant was discarded, and the precipitate was washed twice with PBS buffer. The cells were then treated with 0.5 mL of 1 mol / L NaOH at 37°C for 48 h. 100 μL of the above treatment solution was added to each well of a 96-well plate, and the absorbance at 475 nm was measured. The melanin synthesis inhibition rate (%) was calculated as: [1 - (average absorbance of experimental group / number of cells in experimental group) / (average absorbance of blank control group / number of cells in blank control group)] × 100%. The results are shown in Table 2.

[0037] Table 2. Whitening effects of different test solutions

[0038] Based on the data in Table 2, compared with the comparative example, the composition prepared in the examples has a higher melanin synthesis inhibition rate and a better whitening effect.

[0039] Security test Human skin patch testing was conducted according to the 2015 edition of the "Cosmetic Safety Technical Specifications". Thirty-five volunteers were selected to conduct safety tests on the compositions prepared in Examples 1-3 and Comparative Examples 1-4. The results showed that none of the above products exhibited any suspicious or positive reactions, indicating that all products were safe and showed no adverse reactions.

[0040] Preparation of freeze-dried facial masks The compositions prepared in Examples 1-3 and Comparative Examples 1-4 were selected as mask stock solutions. After soaking the mask rolls with the mask stock solutions, the mask rolls were shaped at -196°C and then freeze-dried at -45°C to obtain freeze-dried masks (the freeze-dried masks were recorded as Examples 1-3 and Comparative Examples 1-4 according to the different mask stock solutions).

[0041] The above-mentioned freeze-dried masks were soaked in water for 10 minutes (the mass ratio of freeze-dried mask to water was 1:10). 2 mL of the soaked mask liquid was taken and evenly applied to a glass plate with microporous breathable tape. The application area was 5 cm × 5 cm. The glass plate was placed in a constant temperature and humidity desiccator. The mass of the glass plate was counted at 2 h and 8 h, and the moisturizing rate was calculated. The results are shown in Table 3. Moisture retention rate (%) = (mass of glass plate after 2h or 8h in the desiccator - mass of glass plate) / (initial mass of glass plate after 2h - mass of glass plate) × 100%.

[0042] Table 3. Moisture retention rates of different test samples

[0043] Based on the data in Table 3, the moisturizing rate of each tested product was above 89% after 2 hours. However, as time went on, the product in the example was able to maintain a higher moisturizing rate, while the moisturizing rate of the product in the comparative example decreased faster.

[0044] Freeze-dried facial mask efficacy test Test environment: Temperature 20℃-22℃, humidity 40%-60%; Test population: The subjects were all aged 18-40 years old, a total of 50 (half male and half female, and the subjects were randomly divided into 5 groups of 10 people each). Test masks: freeze-dried masks prepared in Examples 1-3, Comparative Examples 1 and 2; Test Method: Soak each freeze-dried mask in water for 10 minutes (freeze-dried mask to water ratio 1:10). Use every night after cleansing for 30 consecutive days (use once every other day). Do not use any products (cosmetics or topical medications) on the test area the day before the test. Before the test, subjects must cleanse their faces uniformly and wipe them dry with a tissue. After cleansing, mark the measurement areas on the subject's face. Before the formal test, subjects should sit quietly in a standard room for 30 minutes, without drinking water, in a test-ready position, and remain relaxed. Tests on skin barrier function strengthening and moisturizing levels will be conducted before use and at 10, 20, and 30 days of use. Skin barrier function: The efficacy of the mask in repairing the skin moisturizing barrier was evaluated by transepidermal water loss (TEWL). The TEWL value was measured using a skin barrier capacity tester. The results are shown in Table 4 (the test areas are the average values ​​of both sides of the cheek, and the test values ​​are all average values). Moisturizing level: The epidermal moisture content was measured using a skin moisture meter, and the results are shown in Table 5 (the test areas are the average values ​​of both sides of the cheek, and the test values ​​are all average values).

[0045] Table 4. Results of skin barrier function of different products

[0046] Based on the data in Table 4, Comparative Examples 1 and 2 showed a rebound or increase in TEWL in the later stages of the experiment, indicating only a short-term repair effect. TEWL in all examples continued to decrease over time, demonstrating a stable barrier repair effect; among them, Example 3 showed a faster rate of decrease in the early stages and a stable effect, combining the advantages of rapid onset and long-term stability.

[0047] Table 5. Moisturizing properties of different products

[0048] Based on the data in Table 5, the initial skin moisture content levels of each group were similar, and the differences in moisture content among the groups were not significant after 10 days. As the experimental period lengthened, the increase in moisture content in the comparative examples slowed down significantly in the later stages. The moisture content of each example continued to increase, with Example 1 reaching the highest epidermal moisture content of 60.35% after 30 days, indicating that its ability to increase stratum corneum moisture content and its long-lasting water-locking ability were superior to the other groups.

[0049] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A composition containing recombinant collagen, characterized in that, The composition comprises, by weight parts, the following raw materials: 1-3 parts recombinant collagen, 2-4 parts sodium acrylate copolymer, 0.1-0.5 parts calcium pantothenate, 0.5-1 parts sodium hyaluronate, 0.1-0.3 parts panthenol, 0.1-0.3 parts yellow collagen, 3-5 parts polyglycerol-10 oleate, 2-5 parts glycerin, 5-8 parts jojoba seed oil, 0.5-3 parts lecithin, 0.1-0.5 parts tocopherol, 1.3-1.8 parts palmitoyl pentapeptide-4, 0.1-0.3 parts quinacrine, 1-3 parts licorice root extract, 1-3 parts centella asiatica extract, 2-6 parts tremella fuciformis extract, and 50-70 parts water.

2. The composition according to claim 1, characterized in that, The recombinant collagen includes recombinant type I collagen and recombinant type III collagen, wherein the mass ratio of recombinant type I collagen to recombinant type III collagen is 1:1.5-2.

3. The composition according to claim 1, characterized in that, The licorice extract is an alcohol extract obtained by extraction with a 65%-85% v / v ethanol solution. The Centella asiatica extract is an alcoholic extract obtained by extraction with a 60%-70% v / v ethanol solution.

4. The composition according to claim 1, characterized in that, The preparation method of the Tremella fuciformis extract is as follows: (1) After crushing the fruiting body of Tremella fuciformis, mix it with water to obtain a mixture; (2) The mixture was mixed with Bacillus subtilis for primary fermentation to obtain the primary fermentation product; (3) Mix the primary fermentation product, brewer's yeast liquid and rice flour for secondary fermentation to obtain the secondary fermentation product; (4) The secondary fermentation products were centrifuged and filtered sequentially to obtain the supernatant; the supernatant was mixed with trehalose and then freeze-dried to obtain the tremella extract.

5. The composition according to claim 4, characterized in that, In step (1), the mass-to-volume ratio of the pulverized tremella to water is 1g:40-50mL.

6. The composition according to claim 4, characterized in that, In step (2), the mass ratio of the mixture to Bacillus subtilis is 100:1-2; The effective viable count of Bacillus subtilis is ≥1×10⁻⁶. 7 CFU / g; The primary fermentation temperature is 35-38℃, and the time is 24-48h.

7. The composition according to claim 4, characterized in that, In step (3), the mass ratio of the primary fermentation product, brewer's yeast liquid and rice flour is 100:3-5:1-3; The effective viable count of the brewer's yeast culture is ≥1×10⁻⁶. 6 CFU / mL; The secondary fermentation is carried out at a temperature of 25-30℃ for 24-48 hours.

8. The composition according to claim 4, characterized in that, In step (4), the volume-to-mass ratio of the supernatant to trehalose is 100 mL: 3-5 g.

9. Use of the composition according to any one of claims 1-8 in the preparation of freeze-dried facial masks.

10. A method for preparing a freeze-dried facial mask, characterized in that, Includes the following steps: The composition according to claim 1 is mixed evenly to obtain a mask stock solution; after soaking the mask roll with the mask stock solution, the mask roll is shaped and freeze-dried to obtain a freeze-dried mask.

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