Compositions with local fat pad-reducing effect and methods of making the same, cosmetics

Abstract

The application relates to the cosmetic technical field and relates to a composition with the effect of reducing local fat lumps, a preparation method thereof and a cosmetic. The composition for reducing local fat lumps comprises, in percentage by mass, European horse chestnut seed extract: 0.1-2%; pink pepper essential oil: 0.01-0.1%; caffeine extract: 1-5%; the above composition realizes multi-target synergistic effect; can endow the extract with excellent transdermal penetration performance, significantly improves the bioavailability of active ingredients at the target site, and thus significantly enhances the overall effect of the composition on reducing fat lumps.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and more specifically, to a composition that reduces localized cellulite, a method for preparing the same, and cosmetics. Background Technology

[0002] Cellulite (also known as localized cellulite or excess fat) is a common metabolic disorder of subcutaneous fat tissue, which often occurs in areas such as the thighs and buttocks, and manifests as an uneven, orange-peel-like appearance on the skin surface.

[0003] Currently, the effectiveness of topical products on the market for reducing localized cellulite needs to be improved. Summary of the Invention

[0004] The purpose of this application is to provide a composition that reduces localized cellulite, a method for preparing the same, and a cosmetic.

[0005] In a first aspect, this application provides a composition for reducing localized cellulite, wherein the composition for reducing localized cellulite comprises, by weight percentage: European horse chestnut seed extract: 0.1%-2%; pink pepper essential oil: 0.01%-0.1%; caffeine extract: 1%-5%.

[0006] In the above technical solution, a multi-target synergistic effect is achieved by combining caffeine extract, horse chestnut seed extract and pink pepper essential oil in a specific mass ratio: caffeine promotes fat decomposition, horse chestnut seed extract improves microcirculation and reduces edema, and pink pepper essential oil assists in promoting penetration and synergistic effect.

[0007] In other embodiments of this application, the European horse chestnut seed extract is obtained by extracting European horse chestnut seeds using a supramolecular eutectic solvent; the supramolecular eutectic solvent consists of hydrogen bond acceptors and hydrogen bond donors; the hydrogen bond acceptors include choline chloride; and the hydrogen bond donors include lactic acid.

[0008] In the above technical solution, the European horse chestnut seed extract is prepared using a supramolecular eutectic solvent with choline chloride as the hydrogen bond acceptor and lactic acid as the hydrogen bond donor. This extraction process not only efficiently extracts active ingredients (such as aescin) from horse chestnut seeds, improving their purity and bioactivity, but also endows the extract with excellent transdermal penetration properties, significantly enhancing the bioavailability of active ingredients at their target sites, thereby significantly strengthening the overall effect of the composition in reducing cellulite.

[0009] In other embodiments of this application, the composition for reducing localized cellulite, by weight percentage, comprises: European horse chestnut seed extract: 0.5%-2%; pink pepper essential oil: 0.02%-0.1%; caffeine extract: 2%-5%.

[0010] In the above technical solution, within the further narrowed ratio range, the synergistic effect between the components is further enhanced, and the overall effect of the composition in promoting fat decomposition, improving microcirculation and enhancing skin smoothness is further improved.

[0011] In other embodiments of this application, the composition for reducing localized cellulite, by weight percentage, comprises: European horse chestnut seed extract: 1.0%-2%; pink pepper essential oil: 0.05%-0.1%; caffeine extract: 3%-5%.

[0012] In other embodiments of this application, the composition for reducing localized cellulite, by weight percentage, further includes: bisabolol: 0.02%-1%; papaverine: 0.1%-2%.

[0013] In the above technical solution, bisabolol and papaverine can synergistically work with horse chestnut seed extract, pink pepper essential oil, and caffeine extract to further enhance the composition's effect in reducing localized cellulite. Bisabolol has excellent anti-inflammatory and soothing effects, effectively relieving skin sensitivity and discomfort associated with cellulite; papaverine helps to further promote microcirculation and accelerate the excretion of metabolic products. The addition of both allows the composition to improve skin comfort while reducing fat and shaping the body, achieving a balance between functionality and user experience.

[0014] In other embodiments of this application, optionally, bisabolol: 0.1%-0.2%; papaverine: 0.5%-1%.

[0015] Secondly, this application provides a method for preparing a composition that reduces localized cellulite, comprising: By weight percentage, mix 0.1%-2% of European horse chestnut seed extract, 0.01%-0.1% of pink pepper essential oil, and 1%-5% of caffeine extract.

[0016] In other embodiments of this application, a method for preparing European horse chestnut seed extract includes: The seeds of the European horse chestnut were extracted using an extractant solution to obtain an extract; the extract was then separated and purified.

[0017] In other embodiments of this application, the extractant solution comprises a supramolecular eutectic solvent; the supramolecular eutectic solvent is composed of hydrogen bond acceptors and hydrogen bond donors; the hydrogen bond acceptors include choline chloride; and the hydrogen bond donors include lactic acid.

[0018] In some embodiments of this application, the extraction includes: ultrasonic extraction at 40°C to 50°C for 40 to 70 minutes, with an ultrasonic power of 300 W to 400 W.

[0019] The above technical solution further specifies the optional process parameters for extraction. Within this parameter range, the extraction efficiency of active ingredients from horse chestnut seeds by DES solvent is further improved. This can improve the extraction rate while avoiding degradation of active ingredients caused by excessively high temperature or excessively long ultrasonic time, which is conducive to improving the activity and stability of the extract.

[0020] In other embodiments of this application, the separation and purification includes: diluting the extract with water to disrupt the structure of the supramolecular eutectic solvate, followed by column chromatography and elution with an aqueous ethanol solution, and collecting the eluent.

[0021] The above technical solution specifies the separation and purification steps after extraction, namely, diluting with water to disrupt the supramolecular structure of the DES solvent, followed by purification using column chromatography. This method can effectively remove solvent residues, sugars, and macromolecular impurities from the extract, significantly improving the purity of the target active ingredient (such as aescin), thereby further enhancing the bioactivity of the composition, improving the transdermal penetration performance of the extract, and facilitating the improvement of the bioavailability of the active ingredient at the target site, thus significantly enhancing the overall effect of the composition in reducing fat deposits.

[0022] In other embodiments of this application, the method for preparing European horse chestnut seed extract further includes: after collecting the eluent, performing a vacuum freeze-drying or spray-drying step to obtain European horse chestnut seed extract in dry powder form.

[0023] The above technical solution further specifies that the purified eluent is subjected to vacuum freeze-drying or spray drying to obtain an extract in dry powder form. The extract in dry powder form exhibits better chemical stability.

[0024] Thirdly, this application provides a cosmetic product comprising the composition for reducing localized cellulite provided in any of the foregoing embodiments; or the cosmetic product comprising a composition for reducing localized cellulite prepared by the preparation method of the composition for reducing localized cellulite provided in any of the foregoing embodiments.

[0025] In some embodiments of this application, the cosmetic is in the form of an emulsion; by weight percentage, the cosmetic comprises 1%-7% of a composition having the effect of reducing localized cellulite. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the described embodiments are some embodiments of this application, but not all embodiments.

[0027] Therefore, the following detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0028] Research has revealed that the formation of localized cellulite involves multiple factors, including excessive proliferation and hypertrophy of adipocytes, tissue edema and toxin accumulation caused by microcirculatory disturbances, and degeneration of the dermal connective tissue fibrous structure. Further research found that topical products on the market used to reduce localized cellulite have low transdermal penetration, resulting in low bioavailability of active ingredients at the target site and hindering their effectiveness in reducing cellulite.

[0029] Based on the above research, this application provides a composition that reduces localized cellulite. The composition, by weight percentage, comprises: European horse chestnut seed extract: 0.1%-2%; pink pepper essential oil: 0.01%-0.1%; caffeine extract: 1%-5%.

[0030] In the above technical solution, a multi-target synergistic effect is achieved by combining caffeine extract, horse chestnut seed extract and pink pepper essential oil in a specific mass ratio: caffeine promotes fat decomposition, horse chestnut seed extract improves microcirculation and reduces edema, and pink pepper essential oil assists in promoting penetration and synergistic effect.

[0031] Further, optionally, and exemplary, in some embodiments of this application, the composition for reducing localized cellulite, by weight percentage, comprises: European horse chestnut seed extract: 0.1%, 0.2%, 0.5%, 0.8%, 1.0%, 1.2%, 1.5%, 1.8%, 2% or any two of the aforementioned values; pink pepper essential oil: 0.01%, 0.02%, 0.05%, 0.08%, 0.09%, 0.1% or any two of the aforementioned values; caffeine extract: 1%, 1.2%, 1.5%, 1.8%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% or any two of the aforementioned values.

[0032] In some embodiments of this application, the European horse chestnut seed extract is obtained by extracting European horse chestnut seeds using a supramolecular eutectic solvent; the supramolecular eutectic solvent consists of hydrogen bond acceptors and hydrogen bond donors; the hydrogen bond acceptors include choline chloride; and the hydrogen bond donors include lactic acid.

[0033] In the above technical solution, the European horse chestnut seed extract is prepared using a supramolecular eutectic solvent (DES) with choline chloride as the hydrogen bond acceptor and lactic acid as the hydrogen bond donor. This DES extraction process not only efficiently extracts active ingredients (such as aescin) from horse chestnut seeds, improving their purity and bioactivity, but also endows the extract with excellent transdermal penetration properties, significantly enhancing the bioavailability of active ingredients at the target site, thereby significantly strengthening the overall effect of the composition in reducing cellulite. Optionally, in some embodiments of this application, the composition for reducing localized cellulite, by weight percentage, includes: European horse chestnut seed extract: 0.5%-2%; pink pepper essential oil: 0.02%-0.1%; caffeine extract: 2%-5%.

[0034] In the above technical solution, within the further narrowed ratio range, the synergistic effect between the components is further enhanced, and the overall effect of the composition in promoting fat decomposition, improving microcirculation and enhancing skin smoothness is further improved.

[0035] Optionally, in some embodiments of this application, the composition for reducing localized cellulite, by weight percentage, comprises: European horse chestnut seed extract: 1.0%-2%; pink pepper essential oil: 0.05%-0.1%; caffeine extract: 3%-5%.

[0036] In some embodiments of this application, the composition for reducing localized cellulite, by weight percentage, further includes: bisabolol: 0.02%-1%; papaverine: 0.1%-2%.

[0037] In the above technical solution, bisabolol and papaverine can synergistically work with horse chestnut seed extract, pink pepper essential oil, and caffeine extract to further enhance the composition's effect in reducing localized cellulite. Bisabolol has excellent anti-inflammatory and soothing effects, effectively relieving skin sensitivity and discomfort associated with cellulite; papaverine helps to further promote microcirculation and accelerate the excretion of metabolic products. The addition of both allows the composition to improve skin comfort while reducing fat and shaping the body, achieving a balance between functionality and user experience.

[0038] For example, in some embodiments of this application, the composition for reducing localized cellulite, by weight percentage, further includes: bisabolol: 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.08%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, or any two of the aforementioned values; papaverine: 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2%, or any two of the aforementioned values.

[0039] In some embodiments of this application, optionally, bisabolol: 0.1%-0.2%; papaverine: 0.5%-1%.

[0040] This application provides a method for preparing a composition that reduces localized cellulite, comprising: By weight percentage, mix 0.1%-2% of European horse chestnut seed extract, 0.01%-0.1% of pink pepper essential oil, and 1%-5% of caffeine extract.

[0041] In some embodiments of this application, a method for preparing European horse chestnut seed extract includes: The seeds of the European horse chestnut were extracted using an extractant solution to obtain an extract; the extract was then separated and purified.

[0042] In the above technical solution, a supramolecular eutectic solvent is used for extraction. Compared with traditional organic solvent extraction, this process has advantages such as being green and environmentally friendly, having high extraction efficiency, and operating under mild conditions. Moreover, it can effectively protect heat-sensitive active ingredients from being destroyed, thereby making the extract more active, improving the transdermal penetration performance of the extract, and helping to improve the bioavailability of active ingredients at the target site, thus significantly enhancing the overall effect of the composition in reducing fat deposits.

[0043] In some embodiments of this application, the extractant solution comprises a supramolecular eutectic solvent.

[0044] In some embodiments of this application, the supramolecular eutectic solvent is composed of hydrogen bond acceptors and hydrogen bond donors; the hydrogen bond acceptors include choline chloride; and the hydrogen bond donors include lactic acid.

[0045] In some embodiments of this application, the supramolecular eutectic solvent comprises, by mass percentage: 30%~50% choline chloride; 50%~70% lactic acid.

[0046] For example, in some embodiments of this application, the supramolecular eutectic solvent, by mass percentage, comprises: choline chloride in amounts of 30%, 32%, 35%, 38%, 40%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, or any two of the aforementioned values; and lactic acid in amounts of 50%, 52%, 55%, 56%, 57%, 58%, 60%, 62%, 65%, 68%, 70%, or any two of the aforementioned values.

[0047] In some embodiments of this application, optionally, the supramolecular eutectic solvent comprises, by mass percentage: 35%~45% choline chloride; 55%~65% lactic acid.

[0048] In some embodiments of this application, the extraction includes: ultrasonic extraction at 40°C to 50°C for 40 to 70 minutes, with an ultrasonic power of 300 W to 400 W.

[0049] The above technical solution further specifies the optional process parameters for extraction. Within this parameter range, the extraction efficiency of active ingredients from horse chestnut seeds by DES solvent is further improved. This can improve the extraction rate while avoiding degradation of active ingredients caused by excessively high temperature or excessively long ultrasonic time, which is conducive to improving the activity and stability of the extract.

[0050] For example, in some embodiments of this application, the extraction includes: 40°C, 41°C, 42°C, 43°C, 44°C, 45°C, 46°C, 47°C, 48°C, 49°C, 50°C or any two of the aforementioned values; ultrasonic extraction for 40 minutes, 42 minutes, 45 minutes, 48 ​​minutes, 50 minutes, 52 minutes, 55 minutes, 58 minutes, 60 minutes, 62 minutes, 65 minutes, 68 minutes, 70 minutes or any two of the aforementioned values; and ultrasonic power of 300 W, 310 W, 320 W, 330 W, 340 W, 350 W, 360 W, 370 W, 380 W, 400 W or any two of the aforementioned values.

[0051] Optionally, in some embodiments of this application, the extraction includes extraction at a ratio of 1:8 (g / mL) to 1:12 (g / mL) between European horse chestnut seeds and extractant solution.

[0052] For example, in some embodiments of this application, the extraction includes: extracting according to a ratio of European horse chestnut seed to extractant solution of 1:8 (g / mL), 1:9 (g / mL), 1:10 (g / mL), 1:11 (g / mL), 1:12 (g / mL), or any range between the two aforementioned values.

[0053] Optionally, in some embodiments of this application, the concentration of the extractant solution is 75% to 90% by mass percentage.

[0054] For example, in some embodiments of this application, the concentration of the extractant solution, by mass percentage, is 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, or a range between any two of the foregoing values.

[0055] Further optionally, in some embodiments of this application, the concentration of the extractant solution is 76% to 90% by mass percentage. Further optionally, in some embodiments of this application, the concentration of the extractant solution is 80% to 90% by mass percentage. Further optionally, in some embodiments of this application, the concentration of the extractant solution is 85% to 90% by mass percentage.

[0056] In some embodiments of this application, the separation and purification includes: diluting the extract with water to disrupt the structure of the supramolecular eutectic solvate, followed by column chromatography and elution with an aqueous ethanol solution, and collecting the eluent.

[0057] The above technical solution specifies the separation and purification steps after extraction, namely, diluting with water to disrupt the supramolecular structure of the DES solvent, followed by purification using column chromatography. This method can effectively remove solvent residues, sugars, and macromolecular impurities from the extract, significantly improving the purity of the target active ingredient (such as aescin), thereby further enhancing the bioactivity of the composition, improving the transdermal penetration performance of the extract, and facilitating the improvement of the bioavailability of the active ingredient at the target site, thus significantly enhancing the overall effect of the composition in reducing fat deposits.

[0058] In some embodiments of this application, the method for preparing European horse chestnut seed extract further includes: after collecting the eluent, performing a vacuum freeze-drying or spray-drying step to obtain European horse chestnut seed extract in dry powder form.

[0059] The above technical solution further specifies that the purified eluent is subjected to vacuum freeze-drying or spray drying to obtain an extract in dry powder form. The extract in dry powder form exhibits better chemical stability.

[0060] Exemplary, in some embodiments of this application, the preparation steps of European horse chestnut seed extract include: Step S1, Raw material pretreatment: Remove impurities, moldy and damaged seeds from the European horse chestnut seeds, and rinse the surface dust with clean water. Then dry with low-temperature hot air at 45℃~50℃ until the moisture content is ≤8% to avoid high temperature damage to the internal saponins; Then, use a universal grinder to grind the seeds and pass them through a 40-60 mesh standard sieve to obtain fine horse chestnut seed powder, which is then sealed and kept away from light for later use.

[0061] Step S2: Preparation of supramolecular eutectic extraction solvent (DES): Accurately weigh the hydrogen bond acceptor choline chloride and the hydrogen bond donor lactic acid; mix the two and heat them in a water bath at 55℃~60℃, and stir magnetically for 30 min~60 min; optionally, by mass percentage, the supramolecular eutectic solvent includes: 43.7% choline chloride; 56.3% lactic acid.

[0062] When the system changes from turbid to a homogeneous and transparent liquid, the basic DES solvent is obtained. According to process requirements, add deionized water to the basic DES solvent to adjust the water content to 10% to 25% (to reduce the viscosity of the system and improve the permeation extraction efficiency), stir and mix well to obtain the diluted DES extract for later use.

[0063] Step S3, Targeted Extraction Process (Ultrasound-Assisted Supramolecular Targeted Extraction): Feed the following materials according to the following ratio: horse chestnut seed powder: diluted DES extract = 1:8 to 1:12 (g / mL), and add them to the extraction reactor equipped with stirring and ultrasonic functions.

[0064] The extraction temperature was controlled at 40–50℃ (low temperature throughout the process to prevent hydrolysis and inactivation of aescin); Turn on mechanical stirring (200 r / min to 300 r / min) for ultrasonic-assisted extraction, with an ultrasonic power of 300 W to 400 W and a single extraction time of 40 to 70 min.

[0065] After extraction, the filter residue and DES extraction filtrate are separated by vacuum filtration. The filter residue was extracted twice under the same conditions, and the two filtrates were combined.

[0066] Step S4: Separate the target extract The DES solvent in the filtrate is tightly bound to the active ingredients. By diluting with water and reversing the phase, the active ingredients (such as aescin) can be transferred to the aqueous phase. Add 3 to 5 times the volume of deionized water to the combined filtrate obtained in step S3, and stir thoroughly for 10 to 20 minutes to break the supramolecular hydrogen bond complex structure, so that active substances such as saponins precipitate into the aqueous phase. AB-8 type macroporous adsorption resin was used to pack the column. The diluted feed solution was loaded onto the column for adsorption at a flow rate of 1 BV / h to 2 BV / h. The resin column was first eluted with 2 BV to 3 BV of deionized water to thoroughly remove residual DES solvent, monosaccharides, and water-soluble small molecule impurities. Then, elution was performed with 40% to 60% ethanol aqueous solution, and the ethanol eluent was collected. This component is the target eluent for enriching aescin.

[0067] Step S5: Concentrate, dry, and obtain the finished extract. The eluent obtained in step S4 is transferred to a rotary evaporator and concentrated under reduced pressure at 45℃~55℃ to recover the ethanol solvent and obtain a concentrated extract. The concentrated extract is then subjected to vacuum freeze-drying or low-temperature spray drying and then pulverized through an 80-90 mesh sieve to obtain supramolecular DES directional extraction of European horse chestnut seed dry powder extract.

[0068] Some embodiments of this application provide a cosmetic product comprising the composition provided in any of the foregoing embodiments that has the effect of reducing localized cellulite; or the cosmetic product comprising a composition for reducing localized cellulite prepared by a method for preparing the composition provided in any of the foregoing embodiments that has the effect of reducing localized cellulite.

[0069] In some embodiments of this application, the cosmetic is in the form of an emulsion; by weight percentage, the cosmetic comprises 1%-7% of a composition having the effect of reducing localized cellulite.

[0070] In some embodiments of this application, the cosmetic also includes other common cosmetic excipients.

[0071] Optionally, in some embodiments of this application, the cosmetic product comprises, by weight percentage: 1%-7% of a composition having the effect of reducing localized cellulite, 2%-15% of oils, 0.5%-6% of an emulsifier, 0.1%-1% of a thickener, optionally 0.5%-2% of a preservative, 1%-15% of a moisturizer, 0.05%-5% of an antioxidant, and the balance being deionized water.

[0072] Optionally, in some embodiments of this application, the aforementioned oils include two or more of the following: caprylic / capric triglycerides, mineral oil, isononyl isononanoate, phytosterol low-erucic acid rapeseed oil glycerides, meadowfoam seed oil, sunflower (HELIANTHUSANNUUS) seed wax, cetyl alcohol, stearyl alcohol, cocoa (THEOBROMA CACAO) seed fat, squalane, polydimethylsiloxane, dioctyl carbonate, jojoba oil, and propylheptyl caprylate.

[0073] Optionally, in some embodiments of this application, the emulsifiers mentioned above include one or more combinations of glyceryl stearate, cetearyl alcohol, cetearyl alcohol polyether-20, tris(cetearyl alcohol polyether)-4 phosphate, and hydrogenated lecithin.

[0074] Optionally, in some embodiments of this application, the thickeners mentioned above include one or more combinations of carbomer, sodium polyacryloyl dimethyl taurate, ammonium acryloyl dimethyl taurate / behenol polyether-25 methacrylate cross-linked polymer, acrylate / C10-30 alkanol acrylate cross-linked polymer, gum arabic (ACACIA SENEGAL), sclerotium gum, and pectin.

[0075] Optionally, in some embodiments of this application, the aforementioned moisturizer includes one or more combinations of glycerin, butylene glycol, pentanediol, sodium hyaluronate, erythritol, trehalose, and dextran.

[0076] Optionally, in some embodiments of this application, the preservatives mentioned above include one or more combinations of: capryloyl hydroxamic acid, ethylhexylglycerin, 1,2-hexanediol, 1,2-pentanediol, 1,2-hexanediol, octyl glycol, and phenoxyethanol.

[0077] Optionally, in some embodiments of this application, the antioxidants mentioned above include: butylated hydroxytoluene and pentaerythritol tetra(bis-tert-butylhydroxyhydrogenated cinnamic acid) ester; and at least one of soy isoflavones and green tea polyphenols.

[0078] Exemplary, in some embodiments of this application, the preparation steps of the cosmetic include: Step 1: Add the oil and emulsifier to the oil pan, heat to 75℃~80℃, stir and mix evenly, and keep warm for 3 min~8 min after all the solid raw materials have dissolved. Step 2: Add the thickener, humectant, and water to the aqueous phase pot, heat to 75℃~88℃, and keep warm for 10 min~20 min after the thickener is completely dispersed. Step 3: Add the aqueous phase raw material to the emulsification pot, then add the oil phase raw material, emulsify at high speed for 5 min to 15 min, maintain vacuum and stirring, and keep warm for 2 min to 8 min.

[0079] Step 4: Turn on the cooling to 40℃~50℃, add the neutralizing agent, stir evenly, and homogenize for 1min~3min.

[0080] Step 5: Continue cooling to 30℃~45℃, add preservatives and the composition to reduce local fat deposits, and stir evenly.

[0081] Step 6: Cool down to 30℃~45℃ and discharge the material.

[0082] The cosmetic product prepared in this application is in emulsion form and has the effect of improving localized cellulite, targeting areas of the body prone to cellulite, such as the buttocks, thighs, and abdomen. Further human efficacy testing results show that this composition tightens and smooths the skin, reducing the appearance of uneven skin texture.

[0083] The features and performance of this application will be further described in detail below with reference to embodiments: Examples and Comparative Examples A composition is provided that has the effect of reducing localized cellulite, the composition of which is shown in Table 1 below: Table 1 (wt%)

[0084] Preparation process: Mix the raw materials of each embodiment or comparative example according to the proportions in the table above.

[0085] The INCI names of each raw material are as follows: Pink Pepper Essential Oil INCI: Frankincense (Schinus terebinthifolius) Seed Extract; Caffeine Extract INCI: Coffee Arabica Leaf / Seed Extract; Bisabolol INCI: Bisabolol; Papaverine INCI: Papaverine.

[0086] The preparation steps of the European horse chestnut seed extract in Example 1 include: Step S1, Raw material pretreatment: Remove impurities, moldy and damaged seeds from the European horse chestnut seeds, and rinse the surface dust with clean water. Then dry in a low-temperature hot air at 50℃ until the moisture content is ≤8% to avoid high temperature damage to the internal saponins; Then, a universal grinder is used to grind the seeds, which are then passed through a 50-mesh standard sieve to obtain fine powder of European horse chestnut seeds. The powder is then sealed and kept away from light for later use.

[0087] Step S2: Preparation of supramolecular eutectic extraction solvent (DES): Accurately weigh the hydrogen bond acceptor choline chloride and the hydrogen bond donor lactic acid; mix the two and heat in a 60°C water bath, stirring magnetically for 50 min; by mass percentage, the supramolecular eutectic solvent comprises: 43.7% choline chloride; 56.3% lactic acid.

[0088] When the system changes from turbid to a homogeneous and transparent liquid, the basic DES solvent is obtained. According to process requirements, deionized water is added to the basic DES solvent to adjust the water content to 20wt%, and the mixture is stirred to obtain the diluted DES extract for later use, that is, to prepare an extractant solution with a concentration of 80wt%.

[0089] Step S3, Targeted Extraction Process (Ultrasound-Assisted Supramolecular Targeted Extraction): Feed the following materials according to the following ratio: European horse chestnut seed powder: extractant solution = 1:10 (g / mL), and add them to the extraction reactor equipped with stirring and ultrasonic functions.

[0090] The extraction temperature was controlled at 45℃, mechanical stirring was turned on at 250 r / min, and ultrasonic-assisted extraction was performed with an ultrasonic power of 350W and a single extraction time of 60min.

[0091] After extraction, the filter residue and DES extraction filtrate are separated by vacuum filtration. The filter residue was extracted twice under the same conditions, and the two filtrates were combined.

[0092] Step S4: Separate the target extract Add 4 times the volume of deionized water to the combined filtrate obtained in step S3, stir thoroughly for 15 minutes to break the supramolecular hydrogen bond complex structure, so that active substances such as saponins precipitate into the aqueous phase. AB-8 macroporous adsorption resin was used to pack the column, and the diluted feed solution was loaded onto the column at a flow rate of 2 BV / h. The resin column was first eluted with 3 BV of deionized water to thoroughly remove residual DES solvent, monosaccharides, and water-soluble small molecule impurities. Then, elution was performed with 50% ethanol aqueous solution, and the ethanol eluent was collected. This component is the target eluent for enriching aescin.

[0093] Step S5: Concentrate, dry, and obtain the finished extract. The eluent obtained in step S4 was transferred to a rotary evaporator and concentrated under reduced pressure at 50°C to recover the ethanol solvent and obtain a concentrated extract. The concentrated extract was then subjected to vacuum freeze-drying or low-temperature spray drying and pulverized through an 80-mesh sieve to obtain supramolecular DES-directed extraction of European horse chestnut seed dry powder extract.

[0094] Example 2 The difference from Example 1 is that, by mass percentage, the supramolecular eutectic solvent comprises: 30% choline chloride and 70% lactic acid.

[0095] Example 3 The difference from Example 1 is that, by mass percentage, the supramolecular eutectic solvent comprises: 50% choline chloride and 50% lactic acid.

[0096] Example 4 The difference from Example 1 is that the following material-liquid ratio was used: European horse chestnut seed powder: extractant solution = 1:8 (g / mL), and the mixture was added to an extraction reactor equipped with stirring and ultrasonic functions.

[0097] Example 5 The difference from Example 1 is that the following material-liquid ratio was used: European horse chestnut seed powder: extractant solution = 1:12 (g / mL), and the mixture was added to an extraction reactor equipped with stirring and ultrasonic functions.

[0098] [Performance Testing] The properties of the compositions in each embodiment and comparative example were tested: 1. In vitro fat-reducing efficacy test of *C. elegans*: Nematode testing: Oil Red O is a lipid-soluble diazole dye that specifically binds to neutral lipids and cholesterol esters in nematodes, staining these fats. Most of the fat in nematodes is stored in the intestines and skin-like epidermal cells. Since the nematode body is transparent, the fat content of *C. elegans* can be determined by observing the staining level of Oil Red O within the nematode; a larger / deeper stain indicates a higher fat content. Using ImageJ software to process the Oil Red O staining samples of nematodes allows for the quantification of fat accumulation and distribution within the nematode.

[0099] Experimental steps: (1) Process the test samples. Use sterile double-distilled water as the solvent for water-soluble samples and DMSO as the solvent for non-water-soluble samples. Dilute the cosmetics obtained in each example or comparative example with the corresponding solvent to obtain the test sample. (2) Preparation of NGM from nematodes; Prepare NGM medium: NaCl 3.0g, agar powder 17.0g, peptone 2.5g, cholesterol 5mg, 1M CaCl2 1mL, 1M MgSO4 1mL, 1M KPO4 buffer (pH 6.0) 25mL; add deionized water to 1L.

[0100] Preparation steps: Add NaCl (3g), agar powder (17g), peptone (2.5g), and 975mL of deionized water to a 2L Erlenmeyer flask. Seal the flask with aluminum foil and autoclave at 121℃ for 20 minutes. After the culture medium cools to 55℃, add 1M CaCl2 (1mL), 1M MgSO4 (1mL), 1M KPO4 buffer (25mL), and cholesterol-ethanol solution (5mg cholesterol dissolved in 0.5mL ethanol) in sequence, and gently shake to mix (avoid air bubbles).

[0101] Pour the culture medium into sterile petri dishes; allow it to solidify at room temperature for 30 minutes, then let it stand for 1 hour to remove condensation; invert and store at 4°C; add 10-20 μL of E. coli OP50 overnight culture medium to the center of the solidified NGM plate. Dry at room temperature for 1-2 days until a thin bacterial colony forms.

[0102] (3) Nematode hatching and staining; Wash NGM plates with M9 buffer and collect the worms into centrifuge tubes. Centrifuge (1000-1500 rpm, 1 min), discard the supernatant, and retain the precipitate. Add 1-2 mL of lysis buffer (formula: 1% NaClO + 0.5M NaOH, freshly prepared). Vigorously vortex for 1-5 min, observe adult lysis and embryo release; add 5 times the volume of M9 buffer to stop the reaction. Centrifuge (1500 rpm, 1 min), discard the supernatant. Resuspend the precipitate in M9 buffer and repeat the washing process 3 times.

[0103] Embryo hatching: Suspend the embryos in 1-2 mL of M9 buffer. Incubate with shaking at room temperature (20°C) or at a specific temperature for 12-24 hours. Synchronized L1 larvae are obtained after embryo hatching (which can be directly inoculated onto NGM plates).

[0104] The composition of M9 buffer (1L) is as follows: Na2HPO4·7H2O 6.0g, KH2PO4 3.0g, NaCl 5.0g, MgSO4 1mM (added after sterilization), and deionized water to a final volume of 1L, followed by autoclaving.

[0105] The PBS buffer (pH 7.4) consists of: 8g NaCl, 0.2g KCl, 1.44g Na2HPO4, 0.24g KH2PO4, and deionized water to a final volume of 1L.

[0106] (4) Image capture and quantitative analysis of lipids After Nile Red staining, the nematodes were transferred to a 2% agarose pad (to reduce movement) or 10mM sodium azide was added (for temporary paralysis). Z-stack sequences were captured using a confocal microscope or a wide-field fluorescence microscope (deconvolution required).

[0107] For each sample, photograph ≥10 nematodes (randomly selected), and capture 3 focal planes (head / middle / tail) for each nematode; save the images.

[0108] Semi-automatic analysis based on ImageJ / Fiji; Region of Interest (ROI) drawn along the worm outline using a free selection tool; fluorescence intensity measured. Quantitative analysis of lipids was determined based on fluorescence intensity.

[0109] Inhibition rate = (1 - TI / CI) * 100% -------- (mean total intensity value of CI blank control group, mean total intensity value of TI sample group) The fat-reducing efficacy was evaluated using the inhibition rate described above. Differences between different treatment groups were analyzed using t-tests, with p < 0.05 generally considered statistically significant. Compared to the blank control, a sample was considered to have a significant fat-reducing effect only if its inhibition rate was positive and statistically significant.

[0110] The experimental data obtained from each embodiment and comparative example are shown in Table 2 below: Table 2 Nematode Test Results

[0111] The experimental data in Table 2 above show that: The nematode inhibition rates in all embodiments were significantly higher than those in the comparative examples. This demonstrates that the solution of this application can effectively improve the nematode inhibition rate. The aforementioned nematode inhibition rate can be used to evaluate fat reduction efficacy; the fat reduction efficacy of all embodiments was significant, while the fat reduction efficacy of Comparative Examples 1-2 was not significant. Therefore, it can be shown that the solution of this application can effectively improve fat reduction efficacy.

[0112] 2. Human efficacy test: Ten female volunteers were recruited and used the sample for 56 days under normal conditions. The results were evaluated using instruments. An increase in the skin roughness (SEr) analysis value indicated improved skin roughness; a decrease in the skin smoothness (SEsm) analysis value indicated improved skin smoothness. Compared with the data before using the sample in Example 4 (D0), the differences after 28 days and 56 days of using the sample in Example 4 were statistically significant (P<0.05).

[0113] The test methods are shown in Table 3: Table 3

[0114] The test results are shown in Table 4: Table 4

[0115] The data in the table above shows that: All volunteers showed a positive increase in skin roughness (SEr) values ​​after using the sample from Example 4 for 28 and 56 days, indicating that skin roughness was improved.

[0116] All volunteers showed negative changes in skin smoothness (SEsm) values ​​after using the sample from Example 4 for 28 and 56 days, indicating an improvement in skin smoothness.

[0117] Therefore, after all 10 volunteers used the test sample of Example 4, both the roughness (SEr) and smoothness (SEsm) of their thigh skin changed in the direction of improvement, indicating that the sample has a general and consistent improvement effect on the test population.

[0118] Furthermore, a 28-day follow-up showed significant improvement after using the test samples from Example 4. Volunteers experienced an average improvement of approximately +10.1% in roughness SEr and an average improvement of approximately -11.9% in smoothness SEsm.

[0119] 56-day follow-up: After using the test samples from Example 4, the improvement effect was further enhanced from the 28-day level, and the increase was significant. The average improvement in roughness SEr among volunteers increased to approximately +16.4%, and the average improvement in smoothness SEsm increased to approximately -16.4%.

[0120] It is evident that the 56-day data for all volunteers was superior to the 28-day data, demonstrating a continuous improvement over time, indicating that continued use of the samples presented in this application can achieve better results.

[0121] In summary, the test samples of Example 4, after continuous use for 28 and 56 days, significantly improved the roughness and smoothness of the skin on the thighs, and the improvement effect increased with the extension of use time. These human efficacy test results effectively verify that the samples of this application have significant efficacy in reducing cellulite and improving skin texture.

[0122] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A composition having the effect of reducing localized cellulite, characterized in that, The composition for reducing localized cellulite comprises, by weight percentage: European horse chestnut seed extract: 0.1%-2%; pink pepper essential oil: 0.01%-0.1%; caffeine extract: 1%-5%.

2. The composition according to claim 1 that has the effect of reducing localized cellulite, characterized in that, The composition for reducing localized cellulite comprises, by weight percentage: European horse chestnut seed extract: 0.5%-2%; Pink pepper essential oil: 0.02%-0.1%; Caffeine extract: 2%-5%; Optionally, the European horse chestnut seed extract is obtained by extracting European horse chestnut seeds using a supramolecular eutectic solvent; the supramolecular eutectic solvent is composed of hydrogen bond acceptors and hydrogen bond donors; the hydrogen bond acceptors include choline chloride; and the hydrogen bond donors include lactic acid.

3. The composition according to claim 1, characterized in that, The composition for reducing localized cellulite, by weight percentage, further includes: bisabolol: 0.02%-1%; papaverine: 0.1%-2%; Optionally, bisabolol: 0.1%-0.2%; papaverine: 0.5%-1%.

4. A method for preparing a composition with the effect of reducing localized cellulite according to any one of claims 1-3, characterized in that, include: European horse chestnut seed extract, by weight percentage: 0.1%-2%; Pink pepper essential oil: 0.01%-0.1%; Caffeine extract: 1%-5% mixed.

5. The method for preparing the composition with the effect of reducing localized cellulite according to claim 4, characterized in that, include: The method for preparing the European horse chestnut seed extract includes: European horse chestnut seeds were extracted using an extractant solution to obtain an extract; the extract was then separated and purified. Optionally, the extractant solution comprises a supramolecular eutectic solvent; Optionally, the supramolecular eutectic solvent is composed of a hydrogen bond acceptor and a hydrogen bond donor; the hydrogen bond acceptor includes choline chloride; and the hydrogen bond donor includes lactic acid. Optionally, the supramolecular eutectic solvent, by mass percentage, comprises: 30%~50% choline chloride; 50%~70% lactic acid.

6. The method for preparing the composition with the effect of reducing localized cellulite according to claim 5, characterized in that, include: The extraction includes: ultrasonic extraction at 40℃~50℃ for 40 minutes~70 minutes, with an ultrasonic power of 300 W~400 W; Optionally, the extraction includes: extraction at a ratio of European horse chestnut seed to extractant solution of 1:8 (g / mL) to 1:12 (g / mL); Optionally, the concentration of the extractant solution is 75% to 90% by mass percentage.

7. The method for preparing the composition with the effect of reducing localized cellulite according to claim 5, characterized in that, include: The separation and purification process includes: diluting the extract with water to disrupt the structure of the supramolecular eutectic solvent, followed by column chromatography and elution with an aqueous ethanol solution, and collecting the eluent.

8. The method for preparing the composition with the effect of reducing localized cellulite according to claim 7, characterized in that, include: The method for preparing the European horse chestnut seed extract further includes: after collecting the eluent, performing a vacuum freeze-drying or spray-drying step to obtain the European horse chestnut seed extract in dry powder form.

9. A cosmetic product, characterized in that, The cosmetic includes the composition for reducing localized cellulite as described in any one of claims 1-3; or the cosmetic includes the composition for reducing localized cellulite prepared by the method for preparing the composition for reducing localized cellulite as described in any one of claims 4-8.

10. The cosmetic product according to claim 9, characterized in that, The cosmetic product is in the form of an emulsion; The cosmetic product comprises, by weight percentage, 1%-7% of the composition having the effect of reducing localized cellulite.

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