A traditional Chinese medicine compound anti-hair loss and hair growth composition and a preparation method thereof

Abstract

The application discloses a traditional Chinese medicine compound anti-hair loss and hair strengthening composition and a preparation method thereof, relates to the field of washing and protecting technology, and comprises the synergic compound of 20 kinds of traditional Chinese medicine active ingredients including angelica extract, radix falcaliae extract, cypress leaf extract, radix sophorae flavescens extract, radix salviae miltiorrhizae extract, licorice extract, ginseng extract, ginger root extract, ligustrum lucidum fruit extract, radix etiam extract, mulberry leaf extract, black ganoderma extract, radix chuanxiong extract, gynura extract, radix etiam extract, radix etiam extract, radix etiam extract, radix etiam extract, radix etiam extract, radix etiam extract, radix etiam extract and radix etiam extract. Gradient pH value is used to induce phase transition emulsification, and a cold and hot alternating annealing stabilization process is used. Through grading regulation of the emulsification environment, active ingredients with different solubility characteristics are orderly loaded in a gradient mode. Then, the interface film molecules are rearranged through temperature alternation, so that the anti-hair loss and hair strengthening composition is formed.

Description

Technical Field

[0001] This invention relates to the field of hair care technology, specifically to a traditional Chinese medicine compound for preventing hair loss and strengthening hair, and its preparation method. Background Technology

[0002] With the fast pace of life, increased work pressure, irregular work and rest schedules, and the influence of environmental factors, the number of people experiencing hair loss continues to expand and shows a significant trend towards younger ages. Currently, androgenetic alopecia, seborrheic alopecia, and telogen effluvium have become the most common types of hair loss in clinical practice, and traditional treatment methods have limited effectiveness, making consumers increasingly eager for safe and effective hair loss prevention and care products.

[0003] Currently, hair loss prevention and care products on the market are mainly divided into two categories: chemically synthesized drugs and herbal products. Chemical drugs, represented by minoxidil and finasteride, have clear clinical efficacy, but also significant limitations: finasteride, as an oral hormone regulator, carries the risk of systemic side effects such as decreased sexual function; topical minoxidil can easily cause scalp irritation, contact dermatitis, and localized hirsutism, and there is a significant rebound hair loss phenomenon after discontinuation, making long-term use unsatisfactory in terms of safety and patient compliance. Existing herbal hair loss prevention products mostly use a few traditional Chinese medicine ingredients such as Polygonum multiflorum and ginger in simple physical mixtures, lacking guidance from traditional Chinese medicine theory. Their targets are singular, only achieving basic scalp nourishment or cleansing, and failing to work synergistically from multiple dimensions such as regulating hormone metabolism, improving microcirculation, and repairing the scalp barrier function, resulting in limited overall hair loss prevention effects.

[0004] Furthermore, the emulsification process of traditional hair care compositions also has significant drawbacks: conventional processes often employ a single-stage homogenization emulsification under a single pH condition, with coarse control of process parameters. This fails to take into account the optimal dispersion conditions for different active ingredients from traditional Chinese medicine. For example, flavonoid glycosides have higher solubility and interfacial activity in a slightly alkaline environment, while saponins are more structurally stable under neutral conditions. A single pH condition can easily lead to insufficient dissolution of some active ingredients, uneven interfacial distribution, low encapsulation rate, and poor bioavailability. At the same time, the emulsion obtained by one-step emulsification has a wide particle size distribution and a loose interfacial membrane structure. During long-term storage, stability issues such as stratification, water separation, and release of active ingredients can easily occur, resulting in a short shelf life and ultimately affecting the stable performance of product efficacy and consumer experience. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a traditional Chinese medicine compound for preventing hair loss and strengthening hair, as well as its preparation method. Based on the theory of monarch, minister, assistant and guide in traditional Chinese medicine, the invention targets the inhibition of 5α-reductase activity, improvement of scalp microcirculation, repair of scalp barrier and nourishment and activation of hair follicles. Twenty active ingredients of traditional Chinese medicine are screened and synergistically compounded. A gradient acid-base induced phase transition emulsification combined with alternating hot and cold annealing stabilization process is adopted. By hierarchically controlling the emulsification environment, active ingredients with different solubility properties are loaded in an orderly gradient. Then, by alternating temperature, the interfacial membrane molecules are rearranged to form the hair loss prevention and strengthening composition.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: On the one hand, a traditional Chinese medicine compound composition for preventing hair loss and strengthening hair, in a total of 1000 parts by weight, comprising active compound components of traditional Chinese medicine, scalp care oil and matrix excipients;

[0007] The active compound components of the traditional Chinese medicine, by weight, include: 6-10 parts of Angelica sinensis extract, 12-18 parts of Polygonum multiflorum root extract, 6-10 parts of Platycladus orientalis leaf extract, 3-5 parts of Sophora flavescens root extract, 4-6 parts of Salvia miltiorrhiza root extract, 2-3 parts of Glycyrrhiza uralensis extract, 3-5 parts of Panax ginseng extract, 4-6 parts of Zingiber officinale root extract, 5-7 parts of Ligustrum lucidum fruit extract, 6-8 parts of Eclipta prostrata extract, 3-5 parts of Morus alba leaf extract, 2-4 parts of Ganoderma lucidum extract, 6-10 parts of Ligusticum chuanxiong extract, 5-7 parts of Centella asiatica extract, 2-4 parts of Clematis chinensis extract, 6-10 parts of Panax notoginseng root extract, 8-12 parts of Astragalus membranaceus root extract, 0.8-1.2 parts of Taraxacum mongolicum extract, 0.8-1.2 parts of Sapindus mukorossi extract, and 1.5-2.5 parts of Ophiopogon japonicus extract.

[0008] The scalp care oil is 0.2-0.4 parts of camellia seed oil;

[0009] The matrix excipients include solvents, humectants, and preservatives / conditioners;

[0010] The solvent is deionized water.

[0011] Furthermore, the preferred active compound components of the traditional Chinese medicine are: 8 parts Angelica sinensis extract, 15 parts Polygonum multiflorum root extract, 8 parts Platycladus orientalis leaf extract, 4 parts Sophora flavescens root extract, 5 parts Salvia miltiorrhiza root extract, 2.5 parts Glycyrrhiza uralensis extract, 4 parts Panax ginseng extract, 5 parts Zingiber officinale root extract, 6 parts Ligustrum lucidum fruit extract, 7 parts Eclipta prostrata extract, 4 parts Morus alba leaf extract, 3 parts Ganoderma lucidum extract, 8 parts Ligusticum chuanxiong extract, 6 parts Centella asiatica extract, 3 parts Clematis chinensis extract, 8 parts Panax notoginseng root extract, 10 parts Astragalus membranaceus root extract, 1 part Taraxacum mongolicum extract, 1 part Sapindus mukorossi extract, and 2 parts Ophiopogon japonicus extract.

[0012] The principal ingredients are: Polygonum multiflorum root extract, Platycladus orientalis leaf extract, Astragalus membranaceus root extract, and Panax notoginseng root extract. Polygonum multiflorum root extract nourishes essence and blood, darkens and strengthens hair, and can delay the regression phase of hair follicles and improve the aging state of hair follicles. Platycladus orientalis leaf extract can significantly inhibit 5α-reductase activity, reduce the production of dihydrotestosterone, block the attack of dihydrotestosterone on hair follicles, and inhibit the development of androgenetic alopecia. Astragalus membranaceus root extract replenishes qi and strengthens the exterior, enhances the vitality and resistance of hair follicle cells, and strengthens the hair follicle's ability to retain hair. Panax notoginseng root extract disperses blood stasis, stops bleeding, promotes angiogenesis, activates dormant hair follicles, and promotes new hair regeneration.

[0013] Assistant herbs: Angelica sinensis extract, Ligusticum chuanxiong extract, Salvia miltiorrhiza root extract, and Zingiber officinale root extract are assistant herbs. They have the effects of promoting blood circulation, warming and unblocking meridians, and dilating blood vessels. They can improve scalp microcirculation, increase blood perfusion in the hair follicle area, deliver sufficient oxygen and nutrients to the hair follicle, assist the principal herbs in enhancing hair follicle activity, and consolidate the effects of preventing hair loss and promoting hair growth.

[0014] The adjuvant ingredients include: Ligustrum lucidum extract and Eclipta prostrata extract, which nourish the liver and kidneys, benefit yin and darken hair, enhance hair resilience, and improve dry and yellow hair; Ginseng extract and Ganoderma lucidum extract, which strengthen the body and delay hair follicle aging; Mulberry leaf extract, which disperses wind-heat and reduces scalp oil secretion; Sophora flavescens root extract and Taraxacum mongolicum extract, which clear heat and dry dampness, have anti-inflammatory and antibacterial effects, and reduce dandruff and hair follicle inflammation; Ophiopogon japonicus extract, which nourishes yin and moistens dryness, and relieves scalp dryness; Centella asiatica extract, Clematis chinensis extract, and Glycyrrhiza uralensis extract, which repair the scalp barrier, have anti-inflammatory and soothing effects, and reduce the irritation of the formula.

[0015] Ingredients: Sapindus mukorossi extract is a natural surfactant that gently cleanses excess oil from the scalp and promotes the absorption of active ingredients; Camellia seed oil moisturizes the scalp, provides the hair follicles with the necessary unsaturated fatty acid nutrients, harmonizes the ingredients, and enhances the skin feel.

[0016] The preparation method of the Polygonum multiflorum root extract is as follows: Polygonum multiflorum root material is taken, impurities are removed, washed, moistened, cut into thick slices, and dried. The dried slices are pulverized and passed through a 20-mesh sieve. Ten times the volume (v / w) of 70% ethanol is added, and the mixture is refluxed twice, each time for 2 hours. The extracts are combined, filtered, and concentrated under reduced pressure (temperature ≤60℃, vacuum degree -0.08MPa) to a thick paste with a relative density of 1.1-1.15 (60℃). The paste is then vacuum dried (temperature 60℃, vacuum degree -0.09MPa), pulverized through an 80-mesh sieve, and obtained as Polygonum multiflorum root extract powder containing 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (…). Not less than 3.0%, containing conjugated anthraquinones as emodin ( ) and emodin methyl ether ( The total amount of ( ) shall not be less than 0.20%. Moisture content ≤ 5.0%, total ash content ≤ 8.0%.

[0017] The preparation method of the Platycladus orientalis leaf extract is as follows: Platycladus orientalis leaves are taken, hard stems and impurities are removed, washed, air-dried, pulverized and passed through a 20-mesh sieve, and 12 times (v / w) of 60% ethanol is added. The mixture is refluxed twice, the first time for 2 hours and the second time for 1.5 hours. The extracts are combined, filtered, and concentrated under reduced pressure (temperature ≤60℃, vacuum degree -0.08MPa) to a thick paste with a relative density of 1.08-1.12 (60℃). The paste is then spray-dried (inlet air temperature 180℃, outlet air temperature 85℃) to obtain Platycladus orientalis leaf extract powder containing quercetin ( The content of ash is not less than 1.0%, the moisture content is ≤6.0%, and the total ash content is ≤10.0%.

[0018] The preparation method of the Astragalus root extract is as follows: Take Astragalus membranaceus root, remove impurities, wash, moisten thoroughly, cut into thick slices, dry, take the dried slices, pulverize through a 20-mesh sieve, add 10 times the amount (v / w) of water, decoct twice, 1.5 hours each time, combine the decoctions, filter, concentrate the filtrate to a relative density of 1.08-1.12 (60℃), add ethanol to make the alcohol content reach 70%, let stand for 12 hours, take the supernatant, recover the ethanol, concentrate under reduced pressure (temperature ≤60℃, vacuum degree -0.08MPa) to a thick paste with a relative density of 1.10-1.15 (60℃), vacuum dry (temperature 60℃, vacuum degree -0.09MPa), pulverize through an 80-mesh sieve to obtain Astragalus root extract powder, containing astragaloside A (… The content shall not be less than 0.10%, and shall contain verbascoside ( The content of ash is not less than 0.05%, the moisture content is ≤5.0%, and the total ash content is ≤5.0%.

[0019] The preparation method of the Panax notoginseng root extract is as follows: Panax notoginseng root material is taken, washed, dried, and pulverized through a 40-mesh sieve. Eight times the amount (v / w) of 70% ethanol is added, and the mixture is refluxed three times, each time for 1.5 hours. The extracts are combined, filtered, and concentrated under reduced pressure (temperature ≤55℃, vacuum degree -0.08MPa) to a thick paste with a relative density of 1.05-1.10 (60℃). The paste is then freeze-dried, pulverized through a 100-mesh sieve, and the resulting Panax notoginseng root extract powder contains ginsenoside Rg1 (… ), Ginsenoside Rb1 ( ) and Panax notoginseng saponin R1 ( The total content of the slag is not less than 6.0%, the moisture content is ≤5.0%, and the total ash content is ≤6.0%.

[0020] The preparation method of the Angelica sinensis extract is as follows: Take Angelica sinensis raw material, remove impurities, wash, moisten thoroughly, cut into thin slices, dry, take the dried slices, pulverize and pass through a 20-mesh sieve, add 10 times (v / w) of 70% ethanol, reflux extract twice, 2 hours each time, combine the extracts, filter, concentrate under reduced pressure (temperature ≤60℃, vacuum degree -0.08MPa) to a thick paste with a relative density of 1.10-1.15 (60℃), spray dry (inlet air temperature 180℃, outlet air temperature 80℃) to obtain Angelica sinensis extract powder, containing ferulic acid ( The content of ash must not be less than 0.10%. Moisture content ≤ 5.0%, total ash content ≤ 7.0%.

[0021] The preparation method of the Ligusticum chuanxiong extract is as follows: Take Ligusticum chuanxiong medicinal material, remove impurities, wash, moisten thoroughly, slice thinly, dry, take the dried slices, pulverize and pass through a 20-mesh sieve, add 8 times the amount (v / w) of 80% ethanol, reflux extract twice, 2 hours each time, combine the extracts, filter, concentrate under reduced pressure (temperature ≤60℃, vacuum degree -0.08MPa) to a thick paste with a relative density of 1.08-1.12 (60℃), vacuum dry (temperature 60℃, vacuum degree -0.09MPa), pulverize and pass through an 80-mesh sieve to obtain Ligusticum chuanxiong extract powder, containing ferulic acid ( The content of ash must not be less than 0.15%. Moisture content ≤ 6.0%, total ash content ≤ 6.0%.

[0022] The preparation method of the Salvia miltiorrhiza root extract is as follows: Take Salvia miltiorrhiza root material, remove impurities, wash, moisten thoroughly, cut into thick slices, dry, take the dried slices, pulverize through a 20-mesh sieve, add 12 times (v / w) of 75% ethanol, reflux extract twice, the first time for 2 hours, the second time for 1.5 hours, combine the extracts, filter, concentrate under reduced pressure (temperature ≤55℃, vacuum degree -0.08MPa) to a thick paste with a relative density of 1.08-1.12 (60℃), vacuum dry (temperature 55℃, vacuum degree -0.09MPa), pulverize through an 80-mesh sieve to obtain Salvia miltiorrhiza root extract powder, containing tanshinone IIA (… Not less than 0.20%, containing salvianolic acid B ( The content of ash must not be less than 3.0%. Moisture content ≤ 6.0%, total ash content ≤ 8.0%.

[0023] The preparation method of the ginger root extract is as follows: Take ginger root medicinal material, remove impurities, wash, cut into thick slices, dry, take the dried slices, pulverize and pass through a 20-mesh sieve, add 10 times (v / w) of 80% ethanol, reflux extract twice, 1.5 hours each time, combine the extracts, filter, concentrate under reduced pressure (temperature ≤60℃, vacuum degree -0.08MPa) to a relative density of 1.08-1.10 (60℃), spray dry (inlet air temperature 175℃, outlet air temperature 80℃) to obtain ginger root extract powder containing 6-gingerol ( The content of ash must not be less than 0.60%. Moisture content ≤ 10.0%, total ash content ≤ 6.0%.

[0024] The preparation methods for the extracts of privet fruit, eclipta prostrata (Eclipta prostrata), mulberry leaf, black Ganoderma lucidum, centella asiatica, clematis chinensis, dandelion, ophiopogon japonicus, ginseng, sophora flavescens root, and licorice are as follows: Take the medicinal slices, pulverize them through a 20-mesh sieve, add 12 times (v / w) of water, decoct and extract twice, each time for 1.5 hours, combine the decoctions, filter, concentrate the filtrate to a relative density of 1.08-1.12 (60℃), and spray dry (inlet air temperature 180℃, outlet air temperature 80℃) to obtain the powders of each extract.

[0025] The Sapindus mukorossi extract is obtained by taking the pericarp of Sapindus mukorossi, removing the seeds, drying, pulverizing through a 20-mesh sieve, adding 10 times the amount of water (v / w), and decocting twice, one hour each time. The decoctions are combined, filtered, and the filtrate is concentrated to a relative density of 1.05-1.08 (60℃). The filtrate is then spray-dried (inlet air temperature 180℃, outlet air temperature 85℃) to obtain the Sapindus mukorossi extract powder. The saponin content, determined by ultraviolet spectrophotometry, shall not be less than 20.0% (calculated as oleanolic acid).

[0026] Furthermore, the preservative is p-hydroxyacetophenone, added at 0.4-0.6 parts by weight; the humectant is 1,2-hexanediol, added at 0.4-0.6 parts by weight.

[0027] On the other hand, a method for preparing a traditional Chinese medicine compound for preventing hair loss and strengthening hair includes:

[0028] S1: Clean and sterilize the mixing tank, add the prescribed amount of deionized water into the mixing tank, start stirring and heat up, and add licorice extract, dandelion extract, soapberry extract, ophiopogon extract and sophora root extract in sequence, and keep stirring until the materials are completely dissolved to obtain the aqueous base liquid.

[0029] S2: While maintaining the system temperature and stirring, add the remaining Chinese herbal extracts in sequence, and continue stirring until the system is uniform and free of lumps to obtain a Chinese herbal mixture.

[0030] S3: The prescribed amount of camellia seed oil is preheated and added to the Chinese herbal medicine mixture. The acidity and alkalinity are adjusted by gradient and the mixture is homogenized to complete the three-stage emulsification to obtain the primary emulsion.

[0031] S4: After sealing the primary emulsion, place it in a hot and cold cycling environment for cyclic treatment of refrigeration and room temperature placement to obtain an annealed stable emulsion.

[0032] S5: Cool the annealed and stabilized emulsion to 35-40℃, add p-hydroxyacetophenone and 1,2-hexanediol, stir evenly, and then filter to obtain the herbal compound composition for preventing hair loss and strengthening hair.

[0033] Furthermore, the specific steps of S1 are as follows:

[0034] Stainless steel mixing tanks are selected, and they are first rinsed with pure water, sterilized with high-temperature steam for 30 minutes, and then dried for use to ensure that there is no microbial contamination or impurity residue.

[0035] Add the prescribed amount of deionized water to a clean mixing tank, turn on the anchor mixer, control the speed to 300-500 rpm, turn on the jacket heating, and heat the water at a uniform rate to 45-55℃ and keep it warm.

[0036] Following the principle of prioritizing easily soluble small-molecule components, licorice extract, dandelion extract, soapberry extract, ophiopogon japonicus extract, and sophora flavescens root extract were added sequentially. Each material was stirred for 2 minutes before adding the next. After all materials were added, stirring was maintained for 10-15 minutes until the liquid surface was clear and there were no visible insoluble particles, thus obtaining the aqueous base solution. Low-temperature and uniform heating was used to avoid local overheating that could damage the active ingredients. Adding materials in order of solubility could prevent poorly soluble components from prematurely encapsulating easily soluble components, ensuring complete dissolution of the base aqueous phase and providing a uniform substrate for subsequent compounding.

[0037] Furthermore, the specific steps of S2 are as follows:

[0038] Keep the system temperature stable at 45-55℃ and maintain the stirring speed at 300-500 rpm. Add the following extracts in order of increasing molecular weight and increasing solubility: Angelica sinensis extract, Ligusticum chuanxiong extract, Salvia miltiorrhiza root extract, Zingiber officinale root extract, Morus alba leaf extract, Ligustrum lucidum fruit extract, Eclipta prostrata extract, Centella asiatica extract, Clematis chinensis extract, Ginseng extract, Ganoderma lucidum extract, Panax notoginseng root extract, Astragalus membranaceus root extract, Polygonum multiflorum root extract, and Platycladus orientalis leaf extract.

[0039] Add one herbal extract at a time and stir for 2-3 minutes to allow it to disperse before adding the next one, to prevent the materials from clumping together. After all the materials have been added, continue stirring for 20-40 minutes. Take a sample and observe if there are any lumps or suspended clumps, and you will get a uniform herbal mixture.

[0040] Feeding ingredients according to molecular weight and solubility gradients can maximize the dispersion of various Chinese medicine components, avoid the premature thickening of large molecular colloidal components which would cause small molecular components to aggregate, and ensure that all active ingredients are uniformly dispersed in the aqueous system.

[0041] Furthermore, the specific steps of S3 are as follows:

[0042] Place the prescribed amount of camellia seed oil in a sealed preheated tank, heat it to 45-55℃ using a jacket and keep it warm to ensure that the oil phase temperature is consistent with the water phase and to avoid emulsion breakage due to temperature difference.

[0043] The preheated camellia seed oil was slowly added dropwise to the Chinese herbal medicine mixture at a flow rate of 10-15 ml / min, while stirring at a low speed of 300 rpm during the addition process, so that the oil phase was initially dispersed into small oil droplets.

[0044] Primary emulsification: The pH of the system is slowly adjusted to 7.3-7.7 using a 0.5% (w / w) dilute sodium hydroxide solution. During the adjustment process, stirring is maintained at 200 rpm to avoid excessive local alkalinity that could damage the activity. After adjustment, a high-shear homogenizer is turned on, with the homogenizer head immersed to 2 / 3 of the liquid surface. Homogenize and emulsify at 8000 rpm for 4-6 minutes. In this stage, the solubility and interfacial activity of flavonoid glycosides are significantly enhanced under the slightly alkaline environment. They can migrate directionally to the oil-water interface and arrange to form a dense primary interfacial film, while simultaneously completing the initial refinement of the emulsion.

[0045] Secondary emulsification: Slowly add 0.1 mol / L citrate buffer to the system at a flow rate of 5-8 mL / min to adjust the pH of the system to 6.3-6.7. Adjust the homogenizing speed to 6000 rpm and continue homogenizing and emulsifying for 4-6 minutes. During this stage, the saponin components are most stable in structure and have moderate interfacial activity under neutral conditions. They can be embedded inside the primary interfacial membrane to form a secondary active loading layer, while further refining the emulsion particle size.

[0046] Three-stage emulsification: Continue to add citrate buffer at the same flow rate to adjust the pH of the system to 5.3-5.7. Adjust the homogenizing speed to 4000 rpm and homogenize for 4-6 minutes to complete the final shaping of the phase transition. The final pH of the system matches the natural weakly acidic environment of the human scalp, which can reduce scalp irritation during use. At the same time, low-speed homogenization can eliminate bubbles generated by high shear, making the emulsion structure more regular.

[0047] To verify the above gradient pH emulsification design and clarify the differences in interfacial behavior of various active ingredients under different pH conditions, quercetin from Platycladus orientalis leaves and 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside from Polygonum multiflorum were selected as representative flavonoid glycosides, and total saponins from Sapindus mukorossi and ginsenoside Rg1 were selected as representative saponin components. Their water solubility and n-octanol / water partition coefficient (logP) were determined at three gradient emulsification nodes of pH 5.5, 6.5, and 7.5, respectively.

[0048] The results show that:

[0049] Under slightly alkaline conditions at pH 7.5, the solubility of the two flavonoid glycosides increased by 47%-53% compared to pH 5.5, the logP value approached 0, the interfacial activity was significantly enhanced, and they could preferentially migrate to the oil-water interface and arrange to form a film.

[0050] Under neutral conditions of pH 6.5, the solubility of the two saponin components reaches its peak, and the logP value is in the optimal interfacial assembly range of 0-1, which can stably embed into the inner side of the primary interfacial membrane to form an active loading layer.

[0051] Under weakly acidic conditions of pH 5.5, the solubility of the two types of components decreases slightly, but their molecular structure and emulsion system exhibit optimal stability, making them compatible with the pH environment of the human scalp.

[0052] Furthermore, the specific steps of S4 are as follows:

[0053] The emulsified promulgated emulsion is transferred to a sealed, heat-resistant container. The container is kept sealed throughout the process to prevent contamination by bacteria and evaporation of moisture, while also protecting it from light to prevent photodegradation of active ingredients.

[0054] Place the sealed container in a hot and cold cycle chamber and perform annealing according to the following procedure:

[0055] Cool to 4°C at a constant rate of 0.5°C / minute, and refrigerate at this constant temperature for 1.5-2.5 hours.

[0056] Heat the mixture to 25°C at a constant rate of 0.5°C / min, and leave it at this temperature for 0.8-1.2 hours.

[0057] The above cooling-heating process constitutes a complete cycle, which is repeated 2-3 times to obtain an annealed stable emulsion.

[0058] Temperature alternation induces the rearrangement of surfactants and active molecules in the emulsion interface film, eliminating interfacial stress generated during emulsification, filling interfacial film defects, and forming a denser and more regular interfacial structure. Slow heating and cooling can avoid emulsion demulsification or particle agglomeration caused by sudden temperature changes, ultimately significantly improving the long-term storage stability of the emulsion.

[0059] Furthermore, the specific steps of S5 are as follows:

[0060] Transfer the annealed stabilized emulsion to a clean mixing tank, start stirring, control the speed at 200-300 rpm, circulate cooling water through the jacket, and cool down to 35-40℃ at a rate of 1℃ / min.

[0061] Add p-hydroxyacetophenone and 1,2-hexanediol in sequence, stirring for 5 minutes after each addition to ensure complete dissolution. After all materials have been added, continue stirring for 10-15 minutes to ensure a homogeneous system.

[0062] The material is filtered under pressure through a 200-mesh filter cloth to remove trace aggregates and impurities. The clear and transparent filtrate is collected to obtain the finished product of the traditional Chinese medicine compound for preventing hair loss and strengthening hair.

[0063] Compared with existing technologies, this traditional Chinese medicine compound for preventing hair loss and strengthening hair, and its preparation method, have the following beneficial effects:

[0064] I. This invention follows the theory of monarch, minister, assistant, and guide in traditional Chinese medicine, systematically selecting and proportioning each herb in the dosage. It works synergistically from four dimensions: inhibiting dihydrotestosterone production, improving scalp blood supply, repairing the scalp environment, and activating dormant hair follicles. It has a good effect on improving various common types of hair loss, such as androgenetic alopecia, seborrheic alopecia, and telogen effluvium. While achieving root-cause hair loss prevention and strengthening, it also has multiple effects such as darkening and brightening hair, controlling oil and removing dandruff, soothing and repairing, and nourishing hair. It has rich targets and comprehensive effects.

[0065] II. In the preparation process of this invention, a gradient pH-induced phase transition emulsification process is used to achieve layered and orderly loading of different active ingredients from traditional Chinese medicine, taking into account their solubility characteristics and interfacial activity differences. This process has been verified by measuring the solubility and interfacial activity of active ingredients at different pH levels. This process can drive active ingredients such as flavonoids and saponins to be enriched sequentially at the oil-water interface according to the interfacial activity gradient, improving the effective ingredient dispersion rate and bioavailability. Combined with graded decreasing homogenization treatment, the emulsion particle size distribution is narrower and more uniform, resulting in a delicate skin feel and higher transdermal penetration efficiency. Furthermore, the alternating hot and cold annealing stabilization treatment can promote molecular rearrangement of the emulsion interfacial film to fill interfacial defects, significantly improving the problems of easy layering and water separation and active ingredient separation in traditional emulsions, effectively extending the product shelf life and ensuring long-term stable efficacy.

[0066] Other advantages, objectives and features of the invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination or study, or may be learned from the practice of the invention. Attached Figure Description

[0067] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0068] Figure 1 A flowchart illustrating the preparation method of a compound traditional Chinese medicine for preventing hair loss and strengthening hair;

[0069] Figure 2 This is a flowchart of step S3 in the preparation method of a compound traditional Chinese medicine for preventing hair loss and strengthening hair. Detailed Implementation

[0070] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with the accompanying drawings and preferred embodiments, is provided below.

[0071] This embodiment provides a traditional Chinese medicine compound composition for preventing hair loss and strengthening hair, with a total weight of 1000 parts and the following components:

[0072] Angelica sinensis extract 8 parts, Polygonum multiflorum root extract 15 parts, Platycladus orientalis leaf extract 8 parts, Sophora flavescens root extract 4 parts, Camellia seed oil 0.3 parts, Salvia miltiorrhiza root extract 5 parts, Glycyrrhiza uralensis extract 2.5 parts, Ginseng extract 4 parts, Zingiber officinale root extract 5 parts, Ligustrum lucidum fruit extract 6 parts, Eclipta prostrata extract 7 parts, Morus alba leaf extract 4 parts, Ganoderma lucidum extract 3 parts, Ligusticum chuanxiong extract 8 parts, Centella asiatica extract 6 parts, Clematis chinensis extract 3 parts, Panax notoginseng root extract 8 parts, Astragalus membranaceus root extract 10 parts, Taraxacum mongolicum extract 1 part, Sapindus mukorossi extract 1 part, Ophiopogon japonicus extract 2 parts, p-hydroxyacetophenone 0.5 parts, 1,2-hexanediol 0.5 parts, the remainder being deionized water.

[0073] like Figure 1 As shown, the specific steps of its preparation method are as follows:

[0074] A sterilized stainless steel mixing tank was selected, and the prescribed amount of deionized water was added. Anchor stirring was started, and the speed was set to 400 rpm. The jacket heating was increased to 50℃ at a rate of 1.5℃ / min and held at that temperature. Licorice extract, dandelion extract, soapberry extract, ophiopogon extract, and sophora root extract were added in sequence, stirring for 2 minutes after each addition. After all the extracts were added, stirring was continued for 12 minutes until the solution was clear and free of particles, thus obtaining the aqueous base solution.

[0075] Maintaining the system temperature at 50℃ and the stirring speed at 400 rpm, add the following extracts in sequence: Angelica sinensis extract, Ligusticum chuanxiong extract, Salvia miltiorrhiza root extract, Zingiber officinale root extract, Morus alba leaf extract, Ligustrum lucidum fruit extract, Eclipta prostrata extract, Centella asiatica extract, Clematis chinensis extract, Ginseng extract, Ganoderma lucidum extract, Panax notoginseng root extract, Astragalus membranaceus root extract, Polygonum multiflorum root extract, and Platycladus orientalis leaf extract. Stir for 2.5 minutes after each addition. After all extracts are added, continue stirring for 30 minutes. Take a sample and observe for any clumping to obtain the Chinese herbal medicine mixture.

[0076] like Figure 2 As shown, camellia seed oil was placed in a preheated tank and heated to 50°C. It was then slowly added dropwise to the herbal mixture at a flow rate of 12 ml / min, while maintaining stirring at 300 rpm throughout the addition process. After the addition was complete, a three-stage emulsification process was performed.

[0077] Primary emulsification: Adjust the pH of the system to 7.5 with 0.5% dilute sodium hydroxide solution, stirring at 200 rpm during the adjustment process; turn on the high-shear homogenizer, immerse the homogenizing head to 2 / 3 of the liquid surface, and homogenize at 8000 rpm for 5 minutes to obtain the primary emulsion.

[0078] Secondary emulsification: Add 0.1 mol / L citrate buffer dropwise at a flow rate of 6 mL / min to adjust the pH to 6.5, adjust the homogenizing speed to 6000 rpm, and homogenize for 5 minutes to obtain the secondary emulsion.

[0079] Third-stage emulsification: Continue to add citrate buffer at the same flow rate to adjust the pH to 5.5, adjust the homogenizing speed to 4000 rpm, homogenize for 5 minutes to complete the emulsion setting and obtain the primary emulsion.

[0080] Transfer the primary emulsion to a sealed, light-proof container and place it in a temperature-circulating chamber; cool it down to 4°C at a rate of 0.5°C / min and keep it at that temperature for 2 hours; then heat it up to 25°C at a rate of 0.5°C / min and keep it at that temperature for 1 hour; repeat the above cycle twice to obtain an annealed and stabilized emulsion.

[0081] The annealed and stabilized emulsion was transferred to a mixing tank and stirred at 250 rpm. The jacket was then cooled to 38°C at a rate of 1°C per minute. p-hydroxyacetophenone and 1,2-hexanediol were added sequentially, and stirred for 5 minutes each. After all the emulsions were added, stirring was continued for 12 minutes. The product was then obtained by pressure filtration through a 200-mesh filter cloth. Example 2

[0082] This embodiment provides a traditional Chinese medicine compound composition for preventing hair loss and strengthening hair, with a total weight of 1000 parts and the following components:

[0083] Angelica sinensis extract 6 parts, Polygonum multiflorum root extract 12 parts, Platycladus orientalis leaf extract 6 parts, Sophora flavescens root extract 3 parts, Camellia seed oil 0.2 parts, Salvia miltiorrhiza root extract 4 parts, Glycyrrhiza uralensis extract 2 parts, Ginseng extract 3 parts, Zingiber officinale root extract 4 parts, Ligustrum lucidum fruit extract 5 parts, Eclipta prostrata extract 6 parts, Morus alba leaf extract 3 parts, Ganoderma lucidum extract 2 parts, Ligusticum chuanxiong extract 6 parts, Centella asiatica extract 5 parts, Clematis chinensis extract 2 parts, Panax notoginseng root extract 6 parts, Astragalus membranaceus root extract 8 parts, Taraxacum mongolicum extract 0.8 parts, Sapindus mukorossi extract 0.8 parts, Ophiopogon japonicus extract 1.5 parts, p-hydroxyacetophenone 0.4 parts, 1,2-hexanediol 0.4 parts, with the remainder being deionized water.

[0084] The process parameters and operating procedures of the preparation method are the same as those in Example 1. Example 3

[0085] This embodiment provides a traditional Chinese medicine compound composition for preventing hair loss and strengthening hair, with a total weight of 1000 parts and the following components:

[0086] Angelica sinensis extract 10 parts, Polygonum multiflorum root extract 18 parts, Platycladus orientalis leaf extract 10 parts, Sophora flavescens root extract 5 parts, Camellia seed oil 0.4 parts, Salvia miltiorrhiza root extract 6 parts, Glycyrrhiza uralensis extract 3 parts, Ginseng extract 5 parts, Zingiber officinale root extract 6 parts, Ligustrum lucidum fruit extract 7 parts, Eclipta prostrata extract 8 parts, Morus alba leaf extract 5 parts, Ganoderma lucidum extract 4 parts, Ligusticum chuanxiong extract 10 parts, Centella asiatica extract 7 parts, Clematis chinensis extract 4 parts, Panax notoginseng root extract 10 parts, Astragalus membranaceus root extract 12 parts, Taraxacum mongolicum extract 1.2 parts, Sapindus mukorossi extract 1.2 parts, Ophiopogon japonicus extract 2.5 parts, p-hydroxyacetophenone 0.6 parts, 1,2-hexanediol 0.6 parts, the remainder being deionized water.

[0087] The process parameters and operating procedures of the preparation method are the same as those in Example 1.

[0088] Comparative Example 1

[0089] The only difference between this comparative example and Example 1 is that the Platycladus orientalis leaf extract is removed and the amount of deionized water is increased accordingly. The remaining components and preparation methods are the same as in Example 1.

[0090] Comparative Example 2

[0091] The only difference between this comparative example and Example 1 is that the extracts of Angelica sinensis, Ligusticum chuanxiong, Salvia miltiorrhiza, and Zingiber officinale have been removed, and the amount of deionized water has been increased accordingly. The remaining components and preparation methods are the same as in Example 1.

[0092] Comparative Example 3

[0093] A commercially available mainstream Chinese herbal anti-hair loss liquid lists He Shou Wu extract and ginger extract as its main ingredients.

[0094] Comparative Example 4

[0095] The formulation of this comparative example is exactly the same as that of Example 1. The only difference is that the preparation method adopts the traditional single-acid-base one-time emulsification process: that is, under the condition of fixed pH 6.5, homogenization emulsification is carried out once at 6000 rpm for 15 minutes without gradient acid-base adjustment (i.e., without undergoing primary emulsification at pH 7.5 and tertiary emulsification at pH 5.5), and without cold and hot cycle stabilization treatment (annealing treatment). The remaining post-processing steps are the same as those of Example 1.

[0096] Comparative Example 5

[0097] The only difference between this comparative example and Example 1 is that only the principal drug components (15 parts of Polygonum multiflorum root extract, 8 parts of Platycladus orientalis leaf extract, 10 parts of Astragalus membranaceus root extract, and 8 parts of Panax notoginseng root extract) are retained, while all the assistant drugs (Angelica sinensis extract, Ligusticum chuanxiong extract, Salvia miltiorrhiza root extract, and Zingiber officinale root extract), adjuvant drugs (Ligustrum lucidum extract, Eclipta prostrata extract, Panax ginseng extract, Ganoderma lucidum extract, Morus alba leaf extract, Sophora flavescens root extract, Taraxacum mongolicum extract, Ophiopogon japonicus extract, Centella asiatica extract, Clematis chinensis extract, and Glycyrrhiza uralensis extract) and guiding drug (Sapindus mukorossi extract) are removed. The removed extracts are replaced with an equal amount of maltodextrin to make the total solids content consistent with that of Example 1. The remaining components (Camellia japonica seed oil, p-hydroxyacetophenone, 1,2-hexanediol, and deionized water) and preparation methods are the same as those in Example 1.

[0098] Comparative Example 6

[0099] The only difference between this comparative example and Example 1 is that only the principal drug components (15 parts of Polygonum multiflorum root extract, 8 parts of Platycladus orientalis leaf extract, 10 parts of Astragalus membranaceus root extract, and 8 parts of Panax notoginseng root extract) and the assistant drug components (8 parts of Angelica sinensis extract, 8 parts of Ligusticum chuanxiong extract, 5 parts of Salvia miltiorrhiza root extract, and 5 parts of Zingiber officinale root extract) and all adjuvant drugs (Ligustrum lucidum fruit extract, Eclipta prostrata extract, Ginseng extract, Ganoderma lucidum extract, Morus alba leaf extract, Sophora flavescens root extract, Taraxacum mongolicum extract, Ophiopogon japonicus extract, Centella asiatica extract, Clematis chinensis extract, Glycyrrhiza uralensis extract) and the guiding drug (Sapindus mukorossi extract) are removed. The removed extracts are replaced with an equal amount of maltodextrin to make the total solids content consistent with that of Example 1. The remaining components and preparation methods are the same as those of Example 1.

[0100] Comparative Example 7

[0101] The only difference between this comparative example and Example 1 is that the amount of each herbal extract is reduced to 50% of that in Example 1 (i.e., 4 parts Angelica sinensis extract, 7.5 parts Polygonum multiflorum root extract, 4 parts Platycladus orientalis leaf extract, 2 parts Sophora flavescens root extract, 2.5 parts Salvia miltiorrhiza root extract, 1.25 parts Glycyrrhiza uralensis extract, 2 parts Panax ginseng extract, 2.5 parts Zingiber officinale root extract, 3 parts Ligustrum lucidum fruit extract, 3.5 parts Eclipta prostrata extract, 2 parts Morus alba leaf extract, 1.5 parts Ganoderma lucidum extract, 4 parts Ligusticum chuanxiong extract, 3 parts Centella asiatica extract, 1.5 parts Clematis chinensis extract, 4 parts Panax notoginseng root extract, 5 parts Astragalus membranaceus root extract, 0.5 parts Taraxacum mongolicum extract, 0.5 parts Sapindus mukorossi extract, and 1 part Ophiopogon japonicus extract), and is made up to the same total amount with deionized water. The remaining components and preparation methods are the same as in Example 1.

[0102] Effect verification test

[0103] 5α-Reductase In vitro inhibitory activity assay:

[0104] The inhibition rate of 5α-reductase in each group of samples was determined by ultraviolet spectrophotometry. The reaction system contained testosterone, reduced nicotinamide adenine dinucleotide phosphate, 5α-reductase solution and the sample to be tested. After reacting at 37℃ for 30 minutes, the amount of product generated was measured and the enzyme inhibition rate was calculated. Each group was repeated 3 times and the average value was taken. The results are shown in Table 1.

[0105] Table 1 Comparison of 5α-reductase inhibition rates among different groups of samples

[0106]

[0107] As shown in Table 1, all embodiments of the present invention exhibit excellent inhibitory effects on 5α-reductase, with Example 1 achieving an inhibition rate of 68.7%. The inhibition rate of Comparative Example 1 decreased significantly after removing Platycladus orientalis leaves, demonstrating that Platycladus orientalis leaves are the core component for inhibiting 5α-reductase. The inhibition rate of Comparative Example 2 also decreased after removing the blood-activating components, indicating that the blood-activating components can synergistically enhance the overall enzyme-inhibiting effect. Comparative Example 4, prepared using a traditional process, showed a significantly lower enzyme inhibition rate than Example 1. The inhibition rates of Comparative Example 5 and 6 were only 32.5% and 48.1%, respectively, all significantly lower than Example 1, indicating that adjuvants and guiding agents are not dispensable but indispensable synergistic components in the complete formulation. The enzyme inhibition rate of Comparative Example 7 was 41.3%, significantly lower than Example 1, and the inhibition rate of Comparative Example 8 was 52.4%, still significantly lower than Example 1, demonstrating that the gradient feeding and gradient pH emulsification process of the present invention can effectively improve the dispersibility and bioavailability of active ingredients, significantly enhancing the efficacy under the same formulation.

[0108] Mouse hair growth promotion test:

[0109] Seventy specific pathogen-free black inbred male mice, weighing 18-22 grams, were randomly divided into 7 groups of 10 mice each. Hair removal cream was used to remove hair from the same area on the back of the mice. The corresponding sample was applied twice a day, 0.2 ml each time, for 28 consecutive days. After the experiment, the back skin was taken to make tissue sections, observe the morphology of hair follicles, and measure the hair follicle density and the length of new hair. The results are shown in Table 2.

[0110] Table 2 Comparison of hair growth indices in mice

[0111]

[0112] As shown in Table 2, all embodiments of the present invention significantly promoted hair growth and increased hair follicle density in mice, with effects significantly superior to the comparative examples. Specifically, the new hair length and hair follicle density of Example 1 were approximately twice that of commercially available products, while the new hair length of Comparative Example 5 was only 2.98 mm, and the hair follicle density was only 6.5 follicles / mm². 2The results were comparable to those of the blank control group, demonstrating that although the principal drug alone has certain 5α-reductase inhibitory activity, it is difficult to exert a significant hair growth promoting effect in vivo. It must be synergistically assisted by the adjuvant, assistant, and guide drugs to fully activate hair follicles. The effect of Comparative Example 6 was still significantly lower than that of Example 1, further demonstrating the indispensability of adjuvant and guide drugs in promoting hair growth.

[0113] Emulsion particle size and distribution coefficient determination:

[0114] The average particle size and polydispersity index of the emulsions of Example 1 and Comparative Example 4 were determined using a laser particle size analyzer. Each group was measured in parallel three times, and the average value was taken. The results are shown in Table 3.

[0115] Table 3 Comparison of Emulsion Particle Size and Distribution Characteristics

[0116]

[0117] As shown in Table 3, the emulsion prepared by the gradient pH emulsification process of the present invention has a smaller average particle size and a polydispersity index that is much lower than that of the traditional process. This indicates that the particle size distribution is narrower and the uniformity is better, which is more conducive to the transdermal penetration and absorption of active ingredients. At the same time, the product has a more delicate and refreshing feel.

[0118] Accelerated stability testing:

[0119] The samples from Example 1 and Comparative Example 4 were sealed and placed in a 45°C constant temperature incubator for accelerated stability testing. The tests were conducted continuously for 3 months, and the appearance, layering, and retention rate of active ingredients were observed monthly. The results are shown in Table 4.

[0120] Table 4 Comparison of Accelerated Stability Test Results

[0121]

[0122] As shown in Table 4, after 3 months of accelerated testing, the sample of Example 1 of the present invention remained uniform and stable, with no stratification or water separation, and the retention rate of active ingredients was as high as 91.5%; while Comparative Example 4 showed obvious stratification, precipitation and significant degradation of active ingredients, proving that the alternating hot and cold annealing treatment of the present invention can significantly improve the stability of the emulsion interface structure. Combined with the multi-layer interface film formed by gradient emulsification, it can greatly extend the shelf life of the product and ensure the long-term stability of efficacy.

[0123] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A compound traditional Chinese medicine composition for preventing hair loss and strengthening hair, characterized in that, The total quantity is 1000 parts by weight, including active compound components of traditional Chinese medicine, scalp care oils and base excipients; The active compound components of the traditional Chinese medicine, by weight, include: 6-10 parts of Angelica sinensis extract, 12-18 parts of Polygonum multiflorum root extract, 6-10 parts of Platycladus orientalis leaf extract, 3-5 parts of Sophora flavescens root extract, 4-6 parts of Salvia miltiorrhiza root extract, 2-3 parts of Glycyrrhiza uralensis extract, 3-5 parts of Panax ginseng extract, 4-6 parts of Zingiber officinale root extract, 5-7 parts of Ligustrum lucidum fruit extract, 6-8 parts of Eclipta prostrata extract, 3-5 parts of Morus alba leaf extract, 2-4 parts of Ganoderma lucidum extract, 6-10 parts of Ligusticum chuanxiong extract, 5-7 parts of Centella asiatica extract, 2-4 parts of Clematis chinensis extract, 6-10 parts of Panax notoginseng root extract, 8-12 parts of Astragalus membranaceus root extract, 0.8-1.2 parts of Taraxacum mongolicum extract, 0.8-1.2 parts of Sapindus mukorossi extract, and 1.5-2.5 parts of Ophiopogon japonicus extract. The scalp care oil is 0.2-0.4 parts of camellia seed oil; The matrix additives include solvents, humectants, and preservatives / conditioners; The solvent is deionized water.

2. The traditional Chinese medicine compound composition for preventing hair loss and strengthening hair as described in claim 1, characterized in that, The preferred active compound components of the traditional Chinese medicine are: 8 parts Angelica sinensis extract, 15 parts Polygonum multiflorum root extract, 8 parts Platycladus orientalis leaf extract, 4 parts Sophora flavescens root extract, 5 parts Salvia miltiorrhiza root extract, 2.5 parts Glycyrrhiza uralensis extract, 4 parts Panax ginseng extract, 5 parts Zingiber officinale root extract, 6 parts Ligustrum lucidum fruit extract, 7 parts Eclipta prostrata extract, 4 parts Morus alba leaf extract, 3 parts Ganoderma lucidum extract, 8 parts Ligusticum chuanxiong extract, 6 parts Centella asiatica extract, 3 parts Clematis chinensis extract, 8 parts Panax notoginseng root extract, 10 parts Astragalus membranaceus root extract, 1 part Taraxacum mongolicum extract, 1 part Sapindus mukorossi extract, and 2 parts Ophiopogon japonicus extract.

3. The traditional Chinese medicine compound composition for preventing hair loss and strengthening hair as described in claim 1, characterized in that, The preservative is p-hydroxyacetophenone, and the amount added is 0.4-0.6 parts by weight; the humectant is 1,2-hexanediol, and the amount added is 0.4-0.6 parts by weight.

4. A method for preparing a traditional Chinese medicine compound for preventing hair loss and strengthening hair, used to prepare the traditional Chinese medicine compound for preventing hair loss and strengthening hair as described in any one of claims 1-3, characterized in that, The method includes S1: Clean and sterilize the mixing tank, add the prescribed amount of deionized water into the mixing tank, start stirring and heat up, and add licorice extract, dandelion extract, soapberry extract, ophiopogon extract and sophora root extract in sequence, and keep stirring until the materials are completely dissolved to obtain the aqueous base liquid. S2: While maintaining the system temperature and stirring, add the remaining Chinese herbal extracts in sequence, and continue stirring until the system is uniform and free of lumps to obtain a Chinese herbal mixture. S3: The prescribed amount of camellia seed oil is preheated and added to the Chinese herbal medicine mixture. The acidity and alkalinity are adjusted by gradient and the mixture is homogenized to complete the three-stage emulsification to obtain the primary emulsion. S4: After sealing the primary emulsion, place it in a hot and cold cycling environment for cyclic treatment of refrigeration and room temperature placement to obtain an annealed stable emulsion. S5: Cool the annealed and stabilized emulsion to 35-40℃, add p-hydroxyacetophenone and 1,2-hexanediol, stir evenly, and filter to obtain the Chinese herbal compound composition for preventing hair loss and strengthening hair.

5. The method for preparing a traditional Chinese medicine compound for preventing hair loss and strengthening hair according to claim 4, characterized in that, In step S1, the stirring speed is 300-500 rpm, the heating rate is 1-2℃ / min, the temperature is maintained at 45-55℃, and after all the materials are added, stirring is maintained for 10-15 minutes until completely dissolved.

6. The method for preparing a traditional Chinese medicine compound for preventing hair loss and strengthening hair according to claim 4, characterized in that, In step S2, the system temperature is maintained at 45-55℃ and the stirring speed is 300-500 rpm.

7. The method for preparing a traditional Chinese medicine compound for preventing hair loss and strengthening hair according to claim 4, characterized in that, In step S3, camellia seed oil is preheated to 45-55°C and added to the traditional Chinese medicine mixture at a flow rate of 10-15 ml / min.

8. The method for preparing a traditional Chinese medicine compound for preventing hair loss and strengthening hair according to claim 4, characterized in that, In step S3, three-stage emulsification is achieved through gradient adjustment of pH and graded homogenization, specifically as follows: The pH of the primary emulsification system was adjusted to 7.3-7.7, the homogenization speed was 8000 rpm, and the emulsification time was 4-6 minutes; The pH of the secondary emulsification system was adjusted to 6.3-6.7, the homogenization speed was 6000 rpm, and the emulsification time was 4-6 minutes; The pH of the three-stage emulsification system was adjusted to 5.3-5.7, the homogenization speed was 4000 rpm, and the emulsification time was 4-6 minutes.

9. A method for preparing a traditional Chinese medicine compound for preventing hair loss and strengthening hair according to claim 8, characterized in that, In step S3, the pH is adjusted using a citrate buffer solution, with a dropping rate of 5-8 mL / min, and low-speed stirring is maintained during the dropping process.

10. A method for preparing a traditional Chinese medicine compound for preventing hair loss and strengthening hair according to claim 4, characterized in that, In S4, the refrigeration temperature is 4°C, the single refrigeration time is 1.5-2.5 hours, the single room temperature placement time is 0.8-1.2 hours, and the number of cycles is 2-3.

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