Exosome-loaded controllable sustained-release microneedle and preparation method thereof

Abstract

The application belongs to the field of cosmetics and provides a controllable sustained-release microneedle loaded with exosomes and a preparation method thereof. A mixed solution of HAMA and LAP is injection molded to 40% to 50% of the total height of the needle tip, and photocrosslinking curing is performed to obtain a sustained-release skeleton; the exosomes are added to a mixed solution of low-molecular-weight sodium hyaluronate and trehalose to form a drug loading layer; the drug loading layer is covered on the sustained-release skeleton and is allowed to penetrate under negative pressure; a CMC-Na solution is coated on the surface of the needle tip and is dried to form a coating layer; PVA and glycerol form a backing layer; after complete drying, the mold is removed to obtain the controllable sustained-release microneedle loaded with exosomes. The preparation method provided in the application realizes the stability and stepwise release of the exosomes by constructing a multi-level microneedle system, matches the physiological cycle of skin repair, and the construction process is simple and suitable for industrial production processes.

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