A ceramide-containing composition with anti-wrinkle firming efficacy and use thereof

Abstract

The present application belongs to the technical field of cosmetics, and particularly relates to a ceramide-containing composition with anti-wrinkle and firming effects and application thereof. The composition provided by the present application is compounded by ceramide, boswellia serrata extract, dendrobium nobile flower extract and anabaena flos-aquae extract; the four components construct a multi-dimensional synergistic effect network of 'barrier protection-antioxidant protection-collagen regeneration and firming', and simultaneously realize multiple effects of skin barrier repair, firming and wrinkle lightening, and delay of skin photoaging; there is a significant synergistic effect within a specific ratio range; lack or replacement of any active component or a large deviation from the weight ratio range of the present application will reduce the promotion rate of type I collagen, type III collagen and type XVII collagen, and affect the skin firming and anti-wrinkle effects; the composition has good mildness, and can be widely applied to the preparation of various anti-aging cosmetics such as cosmetic water, essence oil, cream and mask.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a ceramide-containing composition with anti-wrinkle and firming effects and its application. Background Technology

[0002] As public awareness of skincare increases, the market demand for anti-wrinkle and firming skincare products continues to rise, with consumers increasingly demanding higher anti-aging performance and gentleness. The loss of collagen, degradation of elastin, damage to the skin barrier, and hydration imbalance are the core aging mechanisms that lead to skin laxity and wrinkles, and are also key research targets in the field of anti-aging skincare.

[0003] Existing anti-wrinkle and firming products mostly adopt physical film-forming lifting or simple compounding solutions such as high-concentration peptides and single active ingredients, which generally have significant technical defects: physical film-forming lifting effect is short-lived and cannot improve skin aging from the root; high-concentration peptides are expensive and have low cost performance; existing active formulas generally have poor stability and weak skin penetration, resulting in limited anti-wrinkle and firming effects and making it difficult to meet the needs of deep and long-lasting anti-aging.

[0004] Ceramides possess excellent skin barrier repair, moisture retention, and skin hydration improvement properties, effectively aiding in skin aging and enhancing firmness, making them suitable for anti-aging skincare systems. Addressing the shortcomings of existing technologies, this application develops an anti-wrinkle and firming composition containing ceramides. Through the synergistic combination of multiple ingredients, it overcomes the shortcomings of traditional products in terms of poor anti-aging and firming effects, meeting the market's demand for highly effective, long-lasting, and gentle anti-aging skincare. Summary of the Invention

[0005] In view of the above-mentioned defects or improvement needs of the prior art, the present invention provides a ceramide-containing composition with anti-wrinkle and firming effects and its application, thereby solving the problem of poor anti-aging and firming effects of existing skin care products.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides a ceramide-containing composition with anti-wrinkle and firming effects, comprising the following components: ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract; wherein the weight ratio of the ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is (0.6-1.1):(0.3-0.7):(1.7-2.2):(1.4-2).

[0007] Preferably, the weight ratio of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is 0.9:0.5:2:1.7.

[0008] In a second aspect, the present invention provides the use of the composition described in the first aspect in the preparation of cosmetics.

[0009] Preferably, the cosmetic product includes one of the following: toner, essential oil, gel, cream, mask, and spray.

[0010] Thirdly, the present invention provides a cosmetic product with anti-wrinkle and firming effects, comprising the composition described in the first aspect.

[0011] Preferably, it also contains excipients acceptable in the cosmetics industry.

[0012] More preferably, the excipients include at least one of solvent, humectant, pH adjuster, skin conditioner, emulsifier, thickener, solubilizer, preservative and fragrance.

[0013] The beneficial effects of this invention are: This invention provides a ceramide-containing composition with anti-wrinkle and firming effects. The composition is a compound of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract. Ceramide forms a skin barrier on the epidermis, effectively preventing external damage and repairing the skin, thus achieving anti-wrinkle and anti-aging effects. Dendrobium nobile flower extract contains polysaccharides, flavonoids, and other active ingredients, possessing moisturizing and antioxidant properties. It helps the skin retain moisture, improves dryness, neutralizes free radicals, delays skin aging, and reduces the appearance of fine lines and wrinkles. Frankincense seed extract contains volatile oils, tannins, and phenolic compounds, possessing antioxidant effects. Alternaria florida extract acts as an inhibitor of matrix metalloproteinases (MMPs), thereby inhibiting MMP activity and reducing the degradation and loss of type I, III, and XVII collagen from the source.

[0014] Furthermore, ceramides, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract are scientifically compounded according to the weight ratio of this invention. First, ceramides repair the skin barrier and enhance its resistance. Then, frankincense seed extract and Dendrobium nobile flower extract neutralize oxidative damage and reduce collagen destruction. Finally, Alternaria florida extract inhibits collagen degradation and promotes collagen synthesis to reconstruct the dermal support structure, constructing a multi-dimensional synergistic efficacy network of "barrier protection - antioxidant care - collagen regeneration and firming," simultaneously achieving multiple effects such as skin barrier repair, firming and wrinkle reduction, and delaying photoaging. The four components exhibit significant synergistic effects within a specific ratio range. The absence or substitution of any active component, or a significant deviation from the weight ratio range specified in this application, will reduce the enhancement rate of type I, type III, and type XVII collagen in the composition, affecting skin firming and anti-wrinkle effects. Simultaneously, the composition provided by this invention has excellent gentleness and can be widely used in the preparation of various anti-aging cosmetics such as toners, essence oils, creams, and masks. Detailed Implementation

[0015] To further illustrate the technical means and effects of the present invention in achieving the intended purpose, the following detailed description of the specific embodiments, structure, features and effects of the present invention, in conjunction with the composition, is provided below.

[0016] For the following compositions, unless otherwise specified, experimental methods are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise specified, all materials and reagents used are commercially available.

[0017] Some of the raw materials and their sources are as follows: Ceramide was purchased from Guangzhou Jahwa Chemical Co., Ltd.; its trade name is Ceramide NP. The frankincense seed extract was purchased from Guangzhou Jahwa Chemical Co., Ltd.; its trade name is frankincense seed extract. The Dendrobium nobile flower extract was purchased from Guangzhou Jahwa Chemical Co., Ltd.; its trade name is Dendrobium nobile flower extract. The extract of *Alternaria hygroscopica* was purchased from Guangzhou Jahwa Chemical Co., Ltd.; its trade name is *Alternaria hygroscopica* extract.

[0018] Example 1 This embodiment provides a composition comprising the following components: ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract; wherein the weight ratio of the ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is 0.6:0.3:1.7:1.4.

[0019] Example 2 This embodiment provides a composition comprising the following components: ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract; the weight ratio of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is 0.9:0.5:2:1.7.

[0020] Example 3 This embodiment provides a composition comprising the following components: ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract; wherein the weight ratio of the ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is 1.1:0.7:2.2:2.

[0021] Comparative Example 1 This comparative example provides a composition that differs from Example 2 only in that ceramide is not added in Comparative Example 1, and its missing amount is made up with deionized water. All other conditions and preparation methods are the same as in Example 2.

[0022] Comparative Example 2 This comparative example provides a composition that differs from Example 2 only in that frankincense seed extract is not added in Comparative Example 2, and deionized water is used to make up for its missing amount. All other conditions and preparation methods are the same as in Example 2.

[0023] Comparative Example 3 This comparative example provides a composition that differs from Example 2 only in that Dendrobium nobile flower extract is not added in Comparative Example 3, and deionized water is used to make up for the missing amount. All other conditions and preparation methods are the same as in Example 2.

[0024] Comparative Example 4 This comparative example provides a composition that differs from Example 2 only in that Comparative Example 4 does not add Alternaria florida extract, but uses deionized water to make up for its missing amount. All other conditions and preparation methods are the same as in Example 2.

[0025] Comparative Example 5 This comparative example provides a composition that differs from Example 2 only in that an equal amount of *Trifolium repens* extract is used instead of *Dendrobium nobile* flower extract in Comparative Example 5, while the other conditions and preparation methods are the same as in Example 2.

[0026] Comparative Example 6 This comparative example provides a composition that differs from Example 2 only in that the weight ratio of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract in Comparative Example 6 is 0.9:2:0.5:1.7. All other conditions and preparation methods are the same as in Example 2.

[0027] In the present invention, the compositions of Examples 1-3 and Comparative Examples 1-6 were prepared by weighing each component according to the formula amount, mixing and homogenizing to obtain each composition.

[0028] The total weight of each composition in Examples 1-3 is the same as that in Comparative Examples 1-6.

[0029] Application examples, comparison application examples, and blank application examples The compositions prepared in Examples 1-3 were used as active ingredients to prepare essential oils. Specifically, the essential oil of Application Example 1 was prepared using the composition of Example 1, and so on, to obtain the essential oils of Application Examples 1-3; the essential oil of Comparative Application Example 1 was prepared using the composition of Comparative Example 1, and so on, to obtain the essential oils of Comparative Application Examples 1-6; and the essential oil of blank application examples was prepared by replacing the composition with an equal amount of isononyl isononanoate.

[0030] The formula of the essential oil is shown in Table 1. Its preparation method includes the following steps: weigh each component according to Table 1, mix each component at room temperature, stir evenly, and obtain the essential oil.

[0031] Table 1. Composition and Dosage of Essential Oils Composition 8 Isononyl isononanoate Supplement to 100 Cetyl ethylhexanoate 20 Undecane 6.5 Tridecane 3 Vitamin E 0.5 essence 0.1 Test Example 1: Anti-wrinkle and firming effect test of the composition Testing Principle: The collagen system is a core component determining skin elasticity and firmness. Type I collagen constructs the dermal mechanical support framework, induces collagen network reconstruction, improves skin laxity and depressions, and helps stabilize tissue structure and repair wounds; Type III collagen enhances cell activity, strengthens connective tissue extensibility, repairs damaged fibers, and improves signs of aging such as rough and dull skin; Type XVII collagen achieves a stable connection between the epidermis and dermis, constructs a water-locking and anti-irritation protective barrier, and provides firm support to the skin. When the skin is damaged, collagen can activate the body's self-repair process, accelerating wound healing and skin tissue regeneration.

[0032] This test used a UVA+UVB combined irradiation ex vivo skin model (Guangdong Boxi Shaanxi Branch) to evaluate the firming and anti-wrinkle effects of the samples by detecting changes in the content of type I collagen, type III collagen, and type XVII collagen. Test methods 1) Test groups: Blank control (BC): No drug administration, no irradiation treatment; Negative control (NC): No drug administration, UVA (30J / cm²) 2 ) + UVB (50mJ / cm 2 Irradiation treatment; Positive control (PC): administered VC (100 μg / mL) + VE (7 μg / mL), UVA (30 J / cm²). 2 ) + UVB (50mJ / cm 2 Irradiation treatment; Sample group: composition (0.625%, v / v), UVA (30 J / cm²) 2 ) + UVB (50mJ / cm 2 Irradiation treatment; 2) Preparation before testing: Place the ex vivo skin model (24±2mm²) into the culture mold of a 6-well plate, add 3.7mL of culture medium to each well, and incubate at 37℃ in a 5% CO2 incubator, changing the medium daily; 3) Irradiation and drug administration: After 2 days of model culture, the test groups and corresponding treatment conditions were followed. Group BC was not irradiated, but the culture medium was replaced with fresh medium. Group NC was irradiated and the culture medium was replaced with fresh medium. Group PC was irradiated and the culture medium was replaced with medium containing VC+VE. Sample groups were irradiated and the culture medium was replaced with fresh medium. Samples were treated with surface drug administration. After irradiation and drug administration, the model was placed in a CO2 incubator (37℃, 5% CO2) for 24 hours. The above operation was repeated 4 times. After the repeated operation, except for groups BC and NC which had normal daily medium changes, the other groups were drug-treated daily for 3 days. 4) Cleaning: After the incubation is complete, wash the remaining test sample with a sterile PBS solution and wipe away any remaining liquid with a sterile cotton swab. 5) Immunofluorescence assay: The model used for detection was fixed with 4% paraformaldehyde for 24 hours. Immunofluorescence was then performed to detect changes in the content of type I, type III, and type XVII collagen. Images were taken and analyzed under a microscope. Using the blank control group as a baseline, the average relative integrated optical density (IOD) was calculated, and the improvement rate was calculated based on the average IOD. ; Results Analysis: Comparing the improvement rates of type I, type III, and type XVII collagen in the positive control and Examples 1-3 in Table 2, it can be seen that the composition provided by the present invention has significant firming and anti-wrinkle effects, and can effectively delay skin aging. Furthermore, comparing the data from Comparative Examples 1-5 and Example 2, it can be seen that the composition obtained by compounding the raw materials provided by the present invention has better skin firming and anti-wrinkle effects.

[0033] The differences and data between Comparative Examples 1-3 and Comparative Example 6 are as follows: The difference between Comparative Example 6 and Examples 1-3 is that the dosage ratio of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is not within the range specified in this invention, and the data of Comparative Example 6 are all reduced. It can be seen that when the dosage ratio of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is within the specific dosage range of this invention, that is, when the weight ratio of the four is (0.6-1.1):(0.3-0.7):(1.7-2.2):(1.4-2), the compound can synergistically improve the anti-wrinkle and firming effects of the composition. Moreover, when the weight ratio of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is 0.9:0.5:2:1.7, the composition has the best effect.

[0034] Table 2. Test results of the anti-wrinkle and firming efficacy of the composition.

[0035] Test Example 2: Skin Irritation Test Recruit 100 participants aged 20 to 50, in groups of 10, using samples with an area not exceeding 50 mm². 21. A qualified spot test apparatus with a depth of approximately 1 mm; 2. Place the sample into the small chamber of the spot test apparatus, with a volume of approximately 0.020 mL to 0.025 mL; 3. Apply the spot test apparatus containing the sample (essential oils from Application Examples 1-3, Comparative Application Examples 1-6, and the blank application example as samples, and distilled water as the blank control) to the flexor side of the subject's forearms using hypoallergenic adhesive tape, and gently press it with the palm of your hand to ensure even application to the skin, for 24 h; 4. Observe the skin reaction according to the standards in Table 3 at 30 min (after the indentation disappears), 24 h, and 48 h after removing the test sample spot test apparatus, and record the observation results.

[0036] Result determination: If more than 5 subjects have a grade 1 suspected skin reaction, or more than 2 subjects have a grade 2 weak positive skin reaction, or if any subject has a grade 3 or higher adverse skin reaction, the test substance is determined to have an adverse skin reaction on the human body.

[0037] After testing, the essential oils of the present invention in Application Examples 1-3 and Comparative Application Examples 1-6 all showed negative reactions after human patch testing, indicating that they are safe and non-irritating to human skin.

[0038] Table 3 Skin Reaction Scoring Criteria 0 negative reaction 1 Suspicious reaction, only slight erythema 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and papules may be present. 3 Strong positive reaction (herpes reaction): erythema, infiltration, edema, papules; the reaction may extend beyond the test area. 4 Extremely positive reaction (confluent herpes simplex reaction): marked erythema, severe infiltration, edema, confluent herpes simplex; reaction extends beyond the test area. Test Example 3: Essential Oil Test Test substances: essential oils prepared using Application Examples 1-3, Comparative Examples 1-6, and blank application examples.

[0039] Experimental Methods: One hundred participants (aged 30-45) with normal facial skin and no history of cosmetic allergies were selected as volunteers for the anti-wrinkle and firming efficacy test (each 10 volunteers used the same sample). Participants applied the product to their face twice daily, morning and evening, with each application being 0.3 mL. Before application (D0) and on day 28 (D28), under conditions of 21±1℃ and 50±10% humidity, after cleansing their faces and waiting 20 minutes, the total area of ​​facial wrinkles and the R² value were measured using VISIA and an MPA580 skin elasticity analyzer (manufactured by CK GmbH, Germany). The overall anti-wrinkle and firming efficacy of the product on human skin was evaluated, and the R² change rate and the total wrinkle area change rate were calculated using the following formulas: ; In the formula: X D0 R² values ​​for each group of volunteers before using the essential oil; X D28 R2 values ​​for each group of volunteers after 28 days of using the essential oil; ; In the formula: Y D0 Y represents the total wrinkle area of ​​each group of volunteers before using the facial oil;D28 The total wrinkle area of ​​each group of volunteers after 28 days of using the essential oil; Results Analysis: Comparing the data on the change rate of R² and the change rate of total wrinkle area in Application Examples 1-3 in Table 4, it can be seen that the essential oil provided by the present invention has significant firming and anti-wrinkle effects, and can effectively delay skin aging. Furthermore, comparing the data from Application Examples 1-5 and Application Example 2, it can be seen that the composition obtained by compounding the raw materials provided by the present invention has better skin firming and anti-wrinkle effects, thus making the essential oil more effective.

[0040] The differences and data between Comparative Application Examples 1-3 and Comparative Application Example 6 are as follows: The difference between Comparative Application Example 6 and Application Examples 1-3 is that the dosage ratio of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is not within the range specified in this invention. The data for Comparative Application Example 6 are all reduced. It can be seen that when the dosage ratio of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is within the specific dosage range of this invention, that is, when the weight ratio of the four is (0.6-1.1):(0.3-0.7):(1.7-2.2):(1.4-2), the compound can synergistically improve the anti-wrinkle and firming effects of the essential oil. Moreover, when the weight ratio of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is 0.9:0.5:2:1.7, the essential oil has the best effect.

[0041] Table 4. Human Trial Evaluation Data Application Example 1 34.68 27.75 Application Example 2 38.00 31.63 Application Example 3 36.35 29.24 Comparative Application Example 1 19.23 20.79 Comparative Application Example 2 17.47 18.32 Comparative Application Example 3 15.61 16.46 Comparative Application Example 4 20.05 19.08 Comparative Application Example 5 26.72 23.67 Comparative Application Example 6 29.64 25.30 Blank application example 2.86 2.01 The above description is merely a preferred composition of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with preferred compositions, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent compositions without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above compositions based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A ceramide-containing composition with anti-wrinkle and firming effects, characterized in that, It includes the following components: ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract; the weight ratio of the ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is (0.6-1.1):(0.3-0.7):(1.7-2.2):(1.4-2).

2. The composition according to claim 1, characterized in that, The weight ratio of ceramide, frankincense seed extract, Dendrobium nobile flower extract, and Alternaria florida extract is 0.9:0.5:2:1.

7.

3. The use of the composition according to claim 1 or 2 in the preparation of cosmetics.

4. The application as described in claim 3, characterized in that, The cosmetics include one of the following: toner, facial oil, gel, cream, mask, and spray.

5. A cosmetic product with anti-wrinkle and firming effects, characterized in that, Contains the composition described in claim 1 or 2.

6. The cosmetic product as described in claim 5, characterized in that, It also contains excipients acceptable in the cosmetics industry.

7. The cosmetic product as described in claim 6, characterized in that, The excipients include at least one of solvents, humectants, pH adjusters, skin conditioning agents, emulsifiers, thickeners, solubilizers, preservatives, and fragrances.

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