A composition comprising biotin and calcium for promoting bone growth and development

Abstract

The present application provides a composition for promoting bone growth and development, which comprises biotin and calcium. The composition can synergistically promote calcium deposition in the bone, improve the absorption rate of calcium, promote the synthesis of collagen, thereby improving bone density and strength, and effectively promoting bone growth and development.

Description

[0001] This application claims priority to Chinese Application No. 202510554080.3, filed on April 29, 2025, entitled “A composition comprising biotin and calcium for promoting bone growth and development,” the entire contents of which are incorporated herein by reference. Technical Field

[0002] This invention belongs to the field of health products and relates to a composition comprising biotin and calcium for promoting bone growth and development. Background Technology

[0003] Human height growth is the result of bone cell division and proliferation, and depends on the growth and development of the skeleton. The skeleton is an important organ of the human body, its structure including the periosteum, bone tissue, and bone marrow. The periosteum contains abundant nerves and blood vessels, and the bone tissue includes compact bone and spongy bone. From the fetal stage to adulthood, the human skeleton gradually develops, with bone mass continuously increasing. Peak bone mass determines growth mass. Children's height growth during puberty depends on the cyclical growth of various parts of the body and the normal development of bones, organs, and various tissues.

[0004] Calcium is the most abundant mineral in the human body, playing a vital role in various physiological activities such as regulating blood clotting, promoting bone growth, cell proliferation, nerve transmission, and immune responses. Calcium is an essential component of bone tissue, crucial for bone growth, repair, and maintaining bone density. However, simply supplementing with calcium can lead to the body's inability to absorb and utilize it, failing to achieve the desired calcium supplementation effect and even causing side effects such as constipation.

[0005] Biotin is a coenzyme for many carboxylases, participating in various metabolic processes, the synthesis of neurotransmitters, supporting the normal functioning of the nervous system, and providing the necessary energy and material basis for cell growth and proliferation. It supports the active transport of calcium ions, regulates the synthesis of calcium-binding proteins, transports calcium from the intestinal lumen into cells, and then, through calcium pumps on the cell membrane, transports calcium into the bloodstream, thereby increasing calcium absorption. By participating in cell metabolism, it influences the function of intestinal cells and promotes intestinal calcium absorption. As a functional protein, biotin also participates in various intracellular biosynthetic processes and can promote collagen synthesis.

[0006] Combining biotin with calcium can enhance osteoblast activity while appropriately inhibiting osteoclast bone resorption, maintaining a balance between osteoblasts and osteoclasts, thus ensuring calcium deposition in the bone and supporting normal bone growth. Simultaneously, biotin can improve calcium absorption and promote collagen synthesis, thereby increasing bone density and strength, effectively promoting bone growth and development. Summary of the Invention

[0007] The purpose of this invention is to provide a composition comprising biotin and calcium for promoting bone growth and development. This composition has a synergistic effect, enabling calcium deposition in bones, increasing calcium absorption, and promoting collagen synthesis, thereby increasing bone density and strength, and effectively promoting bone growth and development.

[0008] The compositions of the present invention have the following advantages:

[0009] 1. Biotin and calcium work synergistically to promote the absorption, utilization, and conversion of calcium in the human body, thereby enhancing calcium absorption and increasing calcium absorption rate;

[0010] 2. Biotin can improve calcium absorption, promote collagen synthesis, and effectively promote bone growth and development;

[0011] 3. The composition can stimulate osteoblasts, accelerate bone healing, relieve leg pain and cramps, increase bone density and strength, and enhance bone robustness;

[0012] 4. It will not cause side effects such as constipation or bloating;

[0013] 5. The composition can effectively promote the growth and development of adolescents and children, and promote their height increase.

[0014] The compositions of this invention are intended for children and adolescents, especially those in puberty.

[0015] The composition of this invention is designed for people who need calcium supplementation.

[0016] The present invention provides a composition comprising biotin and calcium for promoting bone growth and development.

[0017] In one embodiment, the calcium in the composition of the present invention is one or more of calcium citrate, calcium acetate, calcium lactate, calcium gluconate, calcium citrate-malate, amino acid chelated calcium, peptide chelated calcium, calcium aspartate, calcium lysine, bovine bone calcium, pork bone calcium, fish bone meal, seaweed calcium, New Zealand green lip calcium, natural calcium lactate, α-ketoglutarate calcium, calcium carbonate, and calcium phosphate.

[0018] In one embodiment, the total mass of calcium in the composition of the present invention is 50-1000 mg, such as 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 180, 200, 220, 240, 280, 300, 400, 500, 600, 700, 800, 900, or 1000 mg.

[0019] In one embodiment, the total mass of the calcium in the composition of the present invention is 100-1000 mg.

[0020] In a preferred embodiment, the total mass of calcium in the composition of the present invention is 200-800 mg.

[0021] In a more preferred embodiment, the total mass of calcium in the composition of the present invention is 300-600 mg.

[0022] In one embodiment, the composition of the present invention comprises a combination of calcium citrate and fish bone powder.

[0023] In one embodiment, the composition of the present invention has a ratio of fish bone powder to calcium citrate of 1:1 to 1:30.

[0024] In a preferred embodiment, the composition of the present invention has a ratio of fish bone powder to calcium citrate of 1:1 to 1:20.

[0025] In one embodiment, the composition of the present invention includes the following steps for preparing the fish bone powder: (1) removing the flesh and fat from salmon to obtain salmon bones; (2) drying the salmon bones and then pulverizing them to obtain primary fish bone powder; (3) placing the obtained primary fish bone powder into an aqueous solution containing a complex protease for enzymatic hydrolysis to obtain fish bone material; (4) taking out the enzymatically hydrolyzed fish bone material, washing it, drying it again, and then ultra-finely pulverizing it.

[0026] In one embodiment, the drying temperature in step (2) of the fish bone powder preparation step is 50-90 ℃, preferably 75 ℃.

[0027] In one embodiment, in step (3) of the preparation of the fish bone powder, the complex protease is selected from two or more of papain, trypsin, pepsin, neutral protease, alkaline protease and flavor protease, preferably a combination of alkaline protease and flavor protease.

[0028] In one embodiment, in step (3) of the preparation of the fish bone powder, the enzymatic hydrolysis temperature is 32-48 ℃ and the enzymatic hydrolysis time is 0.5-5 h.

[0029] In one embodiment, the temperature for re-drying in step (4) of the fish bone powder preparation step is 50-90 ℃, preferably 65 ℃.

[0030] Collagen hydrolysates can stimulate an increase in the number and differentiation of osteoblasts, reduce osteoclast activity, and decrease bone resorption, thereby adjusting bone structure and bone mineral distribution, effectively maintaining bone density, increasing the cortical bone area, and increasing bone strength.

[0031] In one embodiment, the composition of the present invention further includes collagen.

[0032] In one embodiment, the composition of the present invention contains one or more of type I collagen, type II collagen, type III collagen, type IV collagen, and type V collagen; further, the collagen is type II collagen.

[0033] In one embodiment, the composition of the present invention comprises one or more of non-denatured type II collagen and hydrolyzed collagen; further, the type II collagen is non-denatured type II collagen.

[0034] In one embodiment, the composition of the present invention contains collagen in a concentration of 5-500 mg, such as 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 180, 200, 220, 240, 280, 300, 400, or 500 mg.

[0035] In one embodiment, the composition of the present invention contains 10-500 mg of collagen.

[0036] In a preferred embodiment, the collagen content of the composition of the present invention is 50-200 mg.

[0037] In a more preferred embodiment, the collagen content of the composition of the present invention is 50-150 mg.

[0038] In one embodiment, the composition of the present invention contains collagen with a molecular weight of 50-500 kDa, such as 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 180, 200, 220, 240, 280, 300, 400, and 500 kDa.

[0039] In a preferred embodiment, the composition of the present invention contains collagen with a molecular weight of 50-300 kDa.

[0040] In a more preferred embodiment, the composition of the present invention contains collagen with a molecular weight of 100-150 kDa.

[0041] In one embodiment, the composition of the present invention contains collagen derived from the skin or bones of cattle, pigs, fish, chickens, or ducks.

[0042] In a preferred embodiment, the collagen in the composition of the present invention is derived from fish bone.

[0043] In a preferred embodiment, the collagen in the composition of the present invention is derived from salmon bones.

[0044] In a particularly preferred embodiment, the composition of the present invention contains collagen derived from fish bone powder.

[0045] In one embodiment, the composition of the present invention has a biotin content ≥0.001 µg / serving.

[0046] In a preferred embodiment, the composition of the present invention contains biotin in a concentration of 0.005-100 µg / part.

[0047] In one embodiment, the composition of the present invention further includes one or more of vitamin D and vitamin K.

[0048] In one embodiment, the composition of the present invention further includes a composition of vitamin D and vitamin K.

[0049] In one embodiment, the vitamin D in the composition of the present invention is one or more of vitamin D2, vitamin D3, vitamin D4, vitamin D5, and vitamin D6.

[0050] Vitamin D3 has a beneficial effect on collagen, promoting the reduction of calcium and phosphate in the blood and soft tissues, and promoting their reabsorption at the gastrointestinal level and return to bone mineral tissue.

[0051] In a preferred embodiment, the vitamin D in the composition of the present invention is vitamin D3.

[0052] In one embodiment, the composition of the present invention contains 50-2000 IU of vitamin D, such as 50, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, or 2000 IU.

[0053] In one embodiment, the composition of the present invention contains 100-2000 IU of vitamin D.

[0054] In a preferred embodiment, the composition of the present invention contains 200-1000 IU of vitamin D.

[0055] In a more preferred embodiment, the composition of the present invention contains 200-800 IU of vitamin D.

[0056] In a particularly preferred embodiment, the composition of the present invention contains 300-500 IU of vitamin D.

[0057] In one embodiment, the vitamin K in the composition of the present invention is one or more of vitamin K2, vitamin K3, and vitamin K4.

[0058] Vitamin K2 can act as a cofactor to promote osteocalcin activation and promote bone mineralization by binding with the same silicon.

[0059] In a preferred embodiment, the vitamin K in the composition of the present invention is vitamin K2.

[0060] In one embodiment, the composition of the present invention contains vitamin K in a concentration of 5-200 µg, such as 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 180, or 200 µg.

[0061] In one embodiment, the composition of the present invention contains 10-200 µg of vitamin K.

[0062] In a preferred embodiment, the composition of the present invention contains 20-100 µg of vitamin K.

[0063] In a more preferred embodiment, the composition of the present invention contains 20-50 µg of vitamin K.

[0064] In one embodiment, the composition of the present invention is prepared by adding excipients to form one or more tablets, capsules, soft capsules, powders, oral liquids, sprays, gummies.

[0065] The liquid is easily absorbed, convenient to take, and will not irritate the gastrointestinal tract.

[0066] In a preferred embodiment, the composition of the present invention is prepared into one or more oral liquids, soft capsules, by adding excipients.

[0067] In a more preferred embodiment, the composition of the present invention is prepared into an oral liquid by adding excipients.

[0068] Biotin also helps maintain normal hair, skin, and nail growth, reducing breakage and brittleness. It can promote the normal functioning of the immune system, enhance the activity of immune cells, improve the body's resistance to disease, and help maintain good health.

[0069] The present invention also provides the use of any of the compositions comprising biotin and calcium in enhancing calcium absorption, promoting collagen formation, and increasing bone toughness.

[0070] The present invention also provides the use of any of the compositions comprising biotin and calcium in improving bone density, bone strength, relieving leg pain and cramps, and enhancing bone robustness.

[0071] The present invention also provides the use of any of the compositions comprising biotin and calcium in promoting growth in adolescents and children.

[0072] The present invention also provides the use of any of the compositions comprising biotin and calcium in promoting hair growth, increasing nail strength, and strengthening teeth and gum health.

[0073] Terminology Explanation

[0074] The "calcium" mentioned refers to the element calcium (Ca). 2+ The determination was performed using inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES), methods that are understandable to those skilled in the art. The calcium may be from a single source or a calcium composition obtained by combining multiple calcium sources.

[0075] The "collagen" referred to is a fibrous protein composed of a tripeptide helix structure and containing large amounts of methionine, proline, and hydroxyproline. The content was determined using spectrophotometry. Specifically, since hydroxyproline is a characteristic amino acid of collagen, those skilled in the art will understand that the content of hydroxyproline in salmon bone collagen was determined spectrophotometrically, and then multiplied by the corresponding conversion factor to obtain the collagen content.

[0076] Biotin, also known as vitamin H, coenzyme R, or vitamin B7, is a water-soluble vitamin. It is a coenzyme for many carboxylases, acting as a CO2 carrier in carboxylase reactions. It is essential for the synthesis of vitamin C and indispensable for the normal metabolism of fats and proteins; it is a necessary nutrient for maintaining natural growth, development, and normal bodily functions. Attached Figure Description

[0077] Figure 1 A bar chart showing the body length of zebrafish after 3 days;

[0078] Figure 2 A bar chart showing the body length of zebrafish after 6 days;

[0079] Figure 3 A bar chart showing the body length of zebrafish after 9 days;

[0080] Figure 4 A bar chart showing the average number of vertebrae in zebrafish after 3 days;

[0081] Figure 5A bar chart showing the average number of vertebrae in zebrafish after 6 days;

[0082] Figure 6 A bar chart showing the average number of vertebrae in zebrafish after 9 days;

[0083] Figure 7 A bar chart showing the fluorescence intensity results of zebrafish vertebrae after 3 days;

[0084] Figure 8 A bar chart showing the fluorescence intensity results of zebrafish vertebrae after 6 days;

[0085] Figure 9 A bar chart showing the fluorescence intensity results of zebrafish vertebrae after 9 days;

[0086] Figure 10 Typical images showing the number of zebrafish vertebrae and fluorescence intensity after processing at 3d, 6d, and 9d. Detailed Implementation

[0087] To facilitate understanding by those skilled in the art, the present invention will be further described below with reference to embodiments. The content mentioned in the embodiments is not intended to limit the present invention.

[0088] Example

[0089] Example 1

[0090] Calcium and biotin are added to obtain a composition.

[0091] Example 2

[0092] The composition is obtained by adding calcium, biotin, collagen, vitamin D, and vitamin K.

[0093] Example 3

[0094] The composition was prepared by adding 500 mg calcium, 0.005 µg biotin, 130 mg collagen, 400 IU vitamin D, and 40 µg vitamin K.

[0095] Example 4

[0096] The composition is obtained by adding calcium, biotin, and collagen.

[0097] Example 5

[0098] Calcium, biotin, and collagen are mixed, and excipients are added. The mixture is then poured into a capsule mold to prepare capsules. The preparation method includes mixing calcium, biotin, and collagen, filling the capsules, sealing them using dry sealing or wet sealing, and finally packaging the capsules.

[0099] Example 6

[0100] Calcium, biotin, and collagen are mixed, and then excipients such as maltodextrin are added to form powder strips. The preparation method involves mixing calcium, biotin, collagen, and other excipients, spray-drying them into powder, and then filling the strips.

[0101] Example 7

[0102] Calcium and biotin are mixed, and appropriate amounts of stabilizers and preservatives such as glycerin and potassium sorbate are added to prepare an oral liquid. The preparation method includes dissolving calcium and biotin, mixing them evenly, adjusting the pH value, filtering to sterilize, filling, and sealing.

[0103] Example 8

[0104] Calcium, biotin, and collagen are mixed, and appropriate amounts of excipients such as calcium bicarbonate and pregelatinized starch are added to form tablets. The preparation method includes unpacking, cleaning, sterilizing, and sieving the excipients; mixing them with calcium, biotin, and collagen; adding a lubricant; inspecting the tableting process; and drying to obtain tablets.

[0105] Example 9

[0106] Calcium, biotin, and collagen are mixed, and appropriate amounts of stabilizers and preservatives such as glycerin and potassium sorbate are added to prepare an oral liquid. The preparation method includes dissolving calcium, biotin, and collagen, mixing them evenly, adjusting the pH value, filtering to sterilize, filling, and sealing.

[0107] Example 10

[0108] Calcium and biotin are mixed, and appropriate amounts of stabilizers and preservatives such as glycerin and potassium sorbate are added to prepare an oral liquid. The preparation method includes using a composite film of a suitable material (such as PET) to obtain strip packets; dissolving calcium and biotin, adding appropriate amounts of stabilizers and preservatives such as glycerin and potassium sorbate, mixing evenly, adjusting the pH value, filtering to sterilize, filling into strip packets, and sealing to obtain the liquid strip packet formulation.

[0109] Example 11

[0110] Salmon is deboned by removing the flesh and fat, yielding the salmon skull and spine. The salmon bones are dried and then pulverized to obtain preliminary fish bone powder. The obtained preliminary fish bone powder is placed in a complex protease aqueous solution containing alkaline protease and flavor protease, and enzymatically hydrolyzed at 35°C for 30 minutes to obtain fish bone feedstock. The feedstock is filtered, washed, dried again, and ultra-finely pulverized to obtain fish bone powder.

[0111] Example 12

[0112] Salmon is deboned by removing the flesh and fat, yielding the salmon skull and spine. The salmon bones are dried and then pulverized to obtain preliminary fish bone powder. The obtained preliminary fish bone powder is placed in a complex protease aqueous solution containing alkaline protease and flavor protease, and enzymatically hydrolyzed at 45°C for 2 hours to obtain fish bone feedstock. The feedstock is filtered, washed, dried again, and ultra-finely pulverized to obtain fish bone powder.

[0113] Example 13

[0114] Salmon is deboned by removing the flesh and fat, yielding the salmon skull and backbone. The salmon bones are dried and then pulverized to obtain preliminary fish bone powder. The obtained preliminary fish bone powder is placed in a complex protease aqueous solution containing alkaline protease and flavor protease, and enzymatically hydrolyzed at 40°C for 40 minutes to obtain fish bone feedstock. The feedstock is filtered, washed, dried again, and ultra-finely pulverized to obtain fish bone powder.

[0115] Example 14

[0116] Salmon is deboned by removing the flesh and fat, yielding the salmon skull and spine. The salmon bones are dried and then pulverized to obtain preliminary fish bone powder. The obtained preliminary fish bone powder is placed in a complex protease aqueous solution containing alkaline protease and flavor protease, and enzymatically hydrolyzed at 48°C for 1 hour to obtain fish bone feedstock. The feedstock is filtered, washed, dried again, and ultra-finely pulverized to obtain fish bone powder.

[0117] Example 15

[0118] Add 1 part fish bone powder and 5 parts calcium citrate to obtain a calcium composition.

[0119] Example 16

[0120] Add 1 part fish bone powder and 1 part calcium citrate to obtain a calcium composition.

[0121] Example 17

[0122] Add 1 part fish bone powder and 10 parts calcium citrate to obtain a calcium composition.

[0123] Example 18

[0124] Add 1 part fish bone powder and 8 parts calcium citrate to obtain a calcium composition.

[0125] Example 19

[0126] Add 1 part fish bone powder and 2 parts calcium citrate to obtain a calcium composition.

[0127] Example 20

[0128] Add 1 part fish bone powder and 3 parts calcium citrate to obtain a calcium composition.

[0129] Biological test cases

[0130] Biological Test Example 1: Zebrafish Growth and Development Experiment

[0131] 1.1. Experimental Materials

[0132] Sample: Prepare a 100 mg / mL solution of sample with DMSO, sonicate and centrifuge, and use the supernatant immediately.

[0133] Experimental animals: Zebrafish were raised in aquarium water at 28 ℃ (water quality: 200 mg of instant sea salt added per 1 L of reverse osmosis water, conductivity 450~550 μS / cm; pH 6.5~8.5; hardness 50~100 mg / L CaCO3), bred and provided by our aquarium.

[0134] Wild-type AB strain zebrafish reproduce through natural pair mating. The optimal age for reproduction is 3 days post-fertilization (3 dpf).

[0135] Instruments, consumables and reagents: Dissecting microscope (SZX7, OLYMPUS, Japan); Stereo fluorescence microscope (MVX10, OLYMPUS, Japan); Precision electronic balance (XSR205, METTLER, USA); 6-well plate (BIOFIL Biotech, China); Dimethyl sulfoxide (DMSO, lot number BCCD8942, Sigma, USA); Calcein (lot number M9870-01, AbMole, USA).

[0136] 1.2. Evaluation of the effect of promoting the growth and development of zebrafish

[0137] Wild-type AB strain zebrafish with a growth factor of 3 dpf were randomly selected and divided into 50 mL beakers, with 100 zebrafish treated in each beaker. The normal control group received no treatment. Group 1 was given calcium, and Group 2 was given the composition of this invention (as in Example 1). After incubation at 28 ℃ for 3, 6, and 9 days, 30 zebrafish from each group were randomly selected, stained with calcein, photographed under a fluorescence microscope, and the images were saved. Data were analyzed and collected using NIS-Elements D 5.41 software. The zebrafish body length, number of vertebrae, and vertebral fluorescence intensity were statistically analyzed. The efficacy of these indicators in promoting zebrafish growth and development was evaluated. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS 26.0 software, and p < 0.05 indicated statistical significance.

[0138] 1.3. Evaluation Results of the Effect of Promoting the Growth and Development of Zebrafish

[0139] The results showed that zebrafish treated with the composition of the present invention exhibited significantly increased body length, enhanced fluorescence intensity of vertebrae, and a significantly increased number of vertebrae at 3, 6, and 9 days post-treatment. This indicates that the composition of the present invention has a significant effect on promoting the growth and development of zebrafish. Detailed results are shown in Tables 1, 2, and 3. Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 , Figure 9 , Figure 10 .

[0140] Table 1. Evaluation results of the body length test on the efficacy of promoting zebrafish growth and development.

[0141] normal control group 3502±30 3979±14 4080±35 1 3592±20 4120±27 4233±22 2 3676±27 4162±33 4296±39

[0142] Table 2. Evaluation results of the experiment on the number of vertebrae promoting zebrafish growth and development.

[0143] normal control group 4.05±0.29 4.59±0.33 5.07±0.40 1 4.25±0.35 5.71±0.47 6.38±0.56 2 4.48±0.32 5.93±0.36 6.54±0.73

[0144] Table 3. Evaluation results of the fluorescence intensity test on the vertebral bone in promoting zebrafish growth and development.

[0145] normal control group 376595±23739 353579±17097 349611±11443 1 424925±23215 420261±28890 421672±17233 2 441536±19414 454958±21977 482227±34267

[0146] It will be understood by those skilled in the art that, unless otherwise defined, all terms used herein (including technical and scientific terms) have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. It should also be understood that terms such as those defined in general dictionaries should be understood to have the same meaning as in the context of the prior art, and should not be interpreted in an idealized or overly formal sense unless specifically defined as herein.

[0147] It should be understood that the above detailed description of the technical solutions of the present invention with reference to preferred embodiments is illustrative and not restrictive. Those skilled in the art can modify the technical solutions described in the embodiments or make equivalent substitutions for some of the technical features based on reading this specification; however, these modifications or substitutions do not cause the essence of the corresponding technical solutions to depart from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition comprising biotin and calcium for promoting bone growth and development.

2. The composition according to claim 1, characterized in that, The calcium is one or more of the following: calcium citrate, calcium acetate, calcium lactate, calcium gluconate, calcium citrate-malate, amino acid chelated calcium, peptide chelated calcium, calcium aspartate, calcium lysine, bovine bone calcium, pork bone calcium, fish bone meal, seaweed calcium, New Zealand green lip calcium, natural calcium lactate, α-ketoglutarate calcium, calcium carbonate, and calcium phosphate.

3. The composition according to claim 2, characterized in that, The total mass of calcium is 50-1000 mg.

4. The composition according to claim 1, characterized in that, It also includes collagen.

5. The composition according to claim 4, characterized in that, The collagen is one or more of type I collagen, type II collagen, type III collagen, type IV collagen, and type V collagen; further, the collagen is type II collagen.

6. The composition according to claim 5, characterized in that... The type II collagen is one or more of non-denatured type II collagen and hydrolyzed collagen; further, the type II collagen is non-denatured type II collagen.

7. The composition according to claim 4, characterized in that, The collagen content is 5-500 mg.

8. The composition according to claim 1, characterized in that, It also includes one or more of vitamin D and vitamin K.

9. The composition according to claim 8, characterized in that, The vitamin D content is 50-2000 IU.

10. The composition according to claim 8, characterized in that, The vitamin K content is 5-200 µg.

11. The composition according to any one of claims 1-10, characterized in that, The composition, when combined with excipients, is prepared into one or more of the following: tablets, capsules, soft capsules, powders, oral liquids, sprays, and gummies.

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