Honey-based medical repair preparation and human ex vivo organ limb soaking type preservation

Abstract

The application discloses a honey-based medical repair preparation and a human body ex vivo organ limb immersion type preservation method, and belongs to the technical field of natural biological medical materials, thermal wound repair and ex vivo transplanted tissue preservation. The application takes high-brix mature honey as a core, and is compounded with vaseline, propolis and licorice extract to prepare an antibiotic-free and chemical additive-free medical paste body, which is used for a first-degree to second-degree thermal burn wound, can relieve pain and inhibit bacteria, promote epithelial regeneration, inhibit scars and reduce pigments. The high penetration and antioxidant properties of honey are used, and sterile honey stock solution is used to soak ex vivo viscera, amputated limbs and transplanted flaps to keep them alive, and can replace UW liquid and HTK liquid. The application realizes the integration of wound repair and ex vivo tissue preservation, solves the problems of traditional burn drugs, such as drug resistance, scarring, high cost of organ preservation liquid, tissue damage, short preservation period, and the like, and has the advantages of technical safety and simplicity, and the values of wound repair and organ transplantation, and wide industrialization prospect.

Description

Technical Field

[0001] This invention relates to the fields of natural biomedical materials, clinical thermal burn wound repair, human organ transplantation, limb replantation, and preservation of ex vivo tissues. Specifically, it relates to the formulation and standardized preparation process of a pure natural honey-based thermal burn repair agent, and also to a long-term preservation and survival medical technology for ex vivo human organs, viscera, limbs, and transplanted soft tissues based on a sterile honey immersion process. It is mainly applied to thermal wound repair in daily life and industrial scenarios, clinical emergency wound treatment, ex vivo transport and preservation of human organs, replantation of severed limbs, and soft tissue defect transplantation and repair, belonging to the field of integrated innovative technology of multifunctional natural medical biomaterials. Background Technology

[0002] Thermal burns, scalds, and scalds are common traumatic injuries in clinical practice, widely occurring in various scenarios such as daily life, industry, and work, with injury levels ranging from superficial first-degree to deep second-degree wounds. Currently, the mainstream clinical repair protocols mostly employ iodine disinfection, topical application of antibiotic ointment, and routine medical dressings. However, these protocols have several inherent technical limitations: long-term use of antibiotics can easily lead to drug resistance in the local bacterial flora, reducing the effectiveness of subsequent repair; after wound healing, pigmentation and abnormal proliferation of fibrous tissue are common, resulting in permanent scarring; and routine dressings have poor breathability and biocompatibility, making the wound prone to fluid accumulation, redness, swelling, and infection. They also have limited effectiveness in repairing diffuse burns from hot water and steam, are only suitable for a limited range of scenarios, and lead to poor postoperative prognosis for patients.

[0003] In the advanced clinical fields of human organ transplantation and limb replantation, the technology of viable preservation of ex vivo tissues is a core key that directly determines the success or failure of surgery and affects postoperative prognosis. After human organs, viscera, and limbs are separated from the maternal blood circulation and physiological microenvironment, they will rapidly undergo a series of irreversible damages such as ischemia and hypoxia, massive accumulation of free radicals, programmed cell apoptosis, tissue edema, and inflammatory degeneration. Tissue necrosis can occur in a short period of time, which seriously limits the timeliness of transplantation surgery and the scope of cross-regional treatment. Currently used clinical artificial chemically synthesized preservation solutions, such as UW solution and HTK solution, have significant technical shortcomings: complex components, stringent preparation requirements, and extremely high procurement and usage costs, making clinical adoption difficult; artificial chemical components have potential immunostimulatory properties, easily inducing microvascular spasm, osmotic pressure imbalance, and oxidative damage in isolated tissues, leading to post-transplant revascularization disorders, exacerbated rejection reactions, and a significant decrease in tissue viability; the preservation window is short, and although conventional cryopreservation can slightly extend the preservation time, it easily causes tissue frostbite and microcirculatory blockage, triggering severe ischemia-reperfusion injury; they lack antibacterial, anti-inflammatory, and tissue repair activities, and are prone to microbial contamination during preservation, increasing the risk of postoperative infection.

[0004] Pure, natural, mature honey possesses multiple natural biological activities, including broad-spectrum antibacterial properties, powerful antioxidant effects, anti-inflammatory and analgesic effects, moisturizing and repairing properties, cell membrane stabilization, inhibition of apoptosis, and improvement of tissue microcirculation. It is also non-toxic, non-irritating, highly biocompatible, causes no immune rejection, and leaves no chemical residues, making it a high-quality natural medical biomaterial. Currently, honey is only used sporadically in the simple care of superficial wounds and as a common food preservative. A standardized, clinically applicable medical burn repair preparation system has not been developed, nor has a sterile immersion preservation technology for excised human organs, viscera, and limbs been developed. The industry has long lacked an integrated technology combining "natural surface wound repair + high-end excised human tissue preservation," and the high-end medical value of honey has not been fully explored and applied. Summary of the Invention

[0005] 3.1 Purpose of the Invention To address the dual shortcomings of existing thermal burn repair techniques, such as drug resistance, susceptibility to wound infection, scarring, and limited repair efficacy, as well as the high cost, poor biocompatibility, risk of secondary tissue damage, short preservation time, and poor transplant outcomes of current clinical human tissue preservation solutions, this invention provides a honey-based medical repair preparation and a method for immersion-based preservation of ex vivo human organs and limbs. This invention optimizes the natural honey compound formula and aseptic preparation process to achieve safe, efficient, and scarless repair of various types of thermal burns. Simultaneously, it constructs a standardized aseptic honey immersion preservation system to achieve long-term safe preservation of ex vivo human organs, viscera, limbs, and transplanted soft tissues, effectively extending the preservation window of ex vivo tissues, significantly improving the success rate of transplantation and replantation surgeries, and filling the technological gap in the integrated use of natural biomaterials for wound repair and medical preservation of ex vivo human organs and limbs.

[0006] This invention comprises two integrated core technology systems, both relying on the natural bioactivity of mature honey. The entire process is purely natural, free of chemical additives, antibiotics, toxicities, and residues, with a high level of biosafety. This achieves an integrated technological innovation for civilian wound repair and high-end clinical transplant preservation. The first system comprises the formula, preparation process, and wound application method of a pure natural honey-based thermal burn repair preparation. The second system comprises a sterile honey immersion method for preserving and preserving excised human organs, viscera, limbs, and transplanted soft tissues.

[0007] (I) Honey-based thermal burn repair preparation technology system 3.2.1 Raw material mass ratio

[0008] The product contains 80-95 parts of pure, natural, mature honey with a Baume degree of 41.5° or higher, 3-8 parts of medical-grade petroleum jelly, 1-3 parts of natural propolis extract, 0.5-1.5 parts of licorice extract, and 1-3 parts of sterile distilled water. All ingredients meet pharmaceutical-grade standards, are free of heavy metals, antibiotics, and chemical residues, and are safe for direct contact with human wounds.

[0009] Optimal formulation: 90 parts pure natural mature honey (42° Baume), 5 parts medical petroleum jelly, 2 parts natural propolis extract, 1 part licorice extract, and 2 parts sterile distilled water. 3.2.2 Standardized Formulation Preparation Process

[0010] (1) Raw material pretreatment: Place mature honey with a Baume degree ≥ 41.5° in a constant temperature and dust-free environment at 40-45℃ for low-speed decrystallization for 20-30 minutes to eliminate crystallized particles and ensure uniform texture of the original liquid; sterile distilled water is sterilized at 121℃ and 0.1MPa for 20 minutes and then cooled for later use; propolis and licorice extract are passed through a 200-mesh sterile ultrafine sieve to remove impurities and retain highly active effective components.

[0011] (2) Constant temperature and low speed homogenization: In a sterile sealed mixing vessel, maintain a constant temperature of 42℃ and stir at a low speed of 60-80r / min. Add sterile distilled water, propolis extract and licorice extract in sequence, stir for 15min until completely blended, then add medical petroleum jelly and stir at a constant temperature for 25-30min to fully emulsify and obtain a fine and uniform medical ointment.

[0012] (3) Low-temperature sterilization and packaging: Sterilize with ultraviolet light for 15-20 minutes at a low temperature of ≤45℃ to fully preserve the natural biological activity of honey. Pack and seal in a Class 10,000 sterile workshop, store at room temperature in the dark and dry place. The product has a shelf life of 36 months. 3.2.3 Standardized Application Process of Thermal Wound Treatment

[0013] This preparation is suitable for three types of thermal injuries: those caused by open flames, hot water, and steam. It covers superficial first-degree burns, superficial second-degree burns, and deep second-degree burns, scalds, and abrasions. Before use, rinse the wound with sterile saline solution to remove dirt, blisters, and fluid, then allow it to drain naturally. No irritating disinfectants such as iodine or alcohol are needed. Apply the preparation evenly to a thickness of 1-2 mm, completely covering the wound and the surrounding 1 cm of normal skin. Superficial wounds should be allowed to air dry for repair. Deep, exudative wounds should be lightly bandaged with sterile dressings for protection. For wounds that have not yet scabbed over, change the dressing every 12 hours. After the wound has scabbed over and the swelling has subsided, change the dressing every 24 hours until the wound is completely healed through epithelialization.

[0014] (II) Aseptic Honey Extract Immersion Preservation Technology System for Human Ex vivo Tissue (Core Innovation) This system uses sterile, purified, mature honey concentrate with a Baume degree of 41.5° or higher as the sole preservation medium. No chemical preservatives are added, and it exhibits excellent biocompatibility, making it suitable for long-term preservation of various isolated medical tissues. The specific operating steps are as follows: (1) Aseptic purification of medical honey stock solution: Select natural mature capped honey with a Baume degree ≥41.5°, place it in an environment of 40-45℃ for low-speed crystallization for 20-30 min, and then sterilize it with ultraviolet light at a low temperature of ≤45℃ for 15-20 min to prepare a sterile, bacteria-free, and biologically active medical honey stock solution, which is then aseptically sealed for later use. Optimal process parameters: constant temperature crystallization at 42℃ for 25 min, low temperature ultraviolet sterilization at 42℃ for 18 min, and use mature honey stock solution with a Baume degree of 42°.

[0015] (2) Aseptic and non-invasive pretreatment of human ex vivo tissues: In a Class 10,000 sterile medical purification environment, freshly removed human ex vivo organs, internal organs, severed limbs, transplanted skin flaps, fascia, blood vessels and nerve tissues are gently rinsed with sterile medical saline to remove surface blood stasis, metabolic impurities and necrotic debris from the cut surface, and the wound is cleaned non-invasively. The residual liquid on the surface is allowed to drain naturally. The entire process is carried out in a standardized aseptic manner to prevent external contamination and secondary damage to the tissues.

[0016] (3) Complete immersion and sealing: The pre-treated human tissue is completely immersed in sterile honey solution, ensuring that the liquid level is 2-3 cm above the top of the tissue, so that the tissue surface, wound surface, and microvascular gaps are fully immersed and wrapped, with no exposed areas; it is placed in a sterile medical sealed container to completely isolate it from air and external sources of pollution, and then sealed and left to stand.

[0017] (4) Low-temperature constant-temperature stable preservation: The sealed soaked tissue is placed in a sterile constant-temperature environment at 2-8℃ for static preservation. The optimal preservation temperature is 4℃, which can stably achieve long-term safe preservation of human tissues for more than 72 hours. Relying on the properties of honey such as high osmotic pressure, broad-spectrum antibacterial, antioxidant, free radical scavenging, anti-apoptosis, cell membrane stabilization, and microcirculation improvement, it effectively inhibits tissue edema, congestion, inflammatory degeneration and cell necrosis, and maintains the physiological activity and structural integrity of isolated tissues for a long time.

[0018] (5) Preoperative activation, removal and rinsing: Before the transplantation surgery, the human tissue preserved in honey is removed and the tissue surface and gaps are rinsed repeatedly and gently with sterile medical saline to thoroughly remove the residual honey solution. After the tissue activation treatment is completed, organ docking, limb anastomosis and soft tissue transplantation repair surgery can be carried out.

[0019] Applicable to: solid organs such as the heart, liver, spleen, kidneys, and lungs; soft tissues of internal organs; severed upper and lower limbs and intact limbs of the hands and feet; skin grafts, fascia, subcutaneous soft tissues, blood vessels, and nerve grafts.

[0020] 1. Safe and efficient thermal wound repair with no drug resistance and no scarring: The pure natural compound preparation of this invention can quickly relieve pain and swelling, reduce inflammation and bacteria, reduce wound exudation, and accelerate epithelial regeneration and healing of the wound. No antibiotics are required throughout the process, completely avoiding the risk of bacterial resistance and skin allergies. It can effectively inhibit wound pigmentation and fibrous scar hyperplasia, and is suitable for all types of thermal burn wounds. The repair effect is significantly better than traditional Western medicine dressings.

[0021] 2. Safe and non-invasive preservation of ex vivo tissues, far surpassing traditional techniques: Pure natural honey liquid is used to completely replace chemical preservation solutions. It has no immune stimulation, no biological toxicity, no residue, and excellent biocompatibility. It can effectively inhibit cell apoptosis, oxidative damage, edema and degeneration in ex vivo tissues, eliminate the problem of bacterial contamination during the preservation process, and significantly extend the safe preservation window period of ex vivo tissues. After transplantation, the tissue blood supply is restored quickly, the postoperative inflammatory response is mild, and the survival quality is high, significantly improving the success rate of organ transplantation and limb replantation surgery.

[0022] 3. Dual-function integrated innovation with high technical barriers: This invention is the first to integrate the dual functions of natural honey material for “surface wound repair + medical preservation of high-end ex vivo organs and limbs”, breaking through the industry shortcomings of existing technologies with fragmented functions and single applications. It has a high degree of technological innovation and covers two major application scenarios: civilian medical emergency and high-end clinical medicine.

[0023] 4. Green, low-cost, easy to mass-produce, and highly applicable to clinical use: The raw materials of this invention are readily available, the preparation process is simple and standardized, no large-scale precision equipment is required, and there are no high consumable costs, so it can be mass-produced on a large scale; the operation process is simple and easy to implement, and it can be widely adapted to various scenarios such as home emergency wound treatment, clinical burn wound repair, operating room organ preservation, and emergency long-distance transport for survival, with extremely high industrialization and promotion value.

[0024] To fully verify the safety, effectiveness, and technological superiority of the dual-function technology system of this invention, multiple standardized controlled trials were conducted based on medical biomaterial testing standards, wound repair efficacy evaluation standards, and ex vivo tissue and organ preservation activity evaluation standards. All trials included parallel control groups, and used standardized testing equipment and evaluation indicators. The data is authentic and traceable. Specific experimental content and quantitative data are as follows:

[0025] 1. Subjects: Thirty patients with clinically confirmed superficial second-degree thermal burns were selected, including 12 cases of hot water scalds, 10 cases of open flame burns, and 8 cases of steam burns. The wound area was 2%-8% of the body surface area. Patients with wound infection, diabetic wounds, and abnormal immune function were excluded. The patients were randomly divided into groups for a controlled trial.

[0026] 2. Experimental Methods: The repair preparation was prepared using the optimal ratio of this invention (90 parts mature honey at 42° Baume, 5 parts medical petroleum jelly, 2 parts propolis extract, 1 part licorice extract, and 2 parts sterile distilled water). The wounds were treated strictly according to standardized procedures. Wounds without scabs were dressed every 12 hours, and wounds with stable scabs were dressed every 24 hours. No antibiotics or irritating disinfectants were used throughout the process. A conventional control group was also included (iodine disinfection + antibiotic ointment + routine sterile dressing). There were no statistically significant differences in wound level, area, or age between the two groups (P > 0.05).

[0027] 3. Quantitative Experiment Results: (1) Analgesic and anti-inflammatory effects: Within 30 minutes after medication, the visual analog scale (VAS) score of wound pain in all patients in the experimental group decreased from the initial 7-9 points to below 2 points, and the redness and swelling of the wound and exudation completely stopped; 24 hours after medication, the expression level of inflammatory factors (TNF-α, IL-6) in the wound decreased by an average of 78.6%, and the anti-inflammatory effect was significant.

[0028] (2) Wound healing cycle: The average healing time for mild thermal wounds (2%-4% area) was 3.8±0.5 days, and the average epithelialization healing time for moderate wounds (4%-8% area) was 8.2±1.2 days, which was more than 35% shorter than the traditional control group.

[0029] (3) Prognosis and repair effect: The wound healing rate of all experimental groups was 100%, with no complications such as infection, suppuration, or ulceration; after 3 months of follow-up, the incidence of wound pigmentation was 0, the incidence of scar hyperplasia was 0, and the smoothness and elasticity of the wound skin recovered to more than 95% of normal skin. The incidence of scar hyperplasia in the traditional control group was 46.7%, and the incidence of pigmentation was 53.3%.

[0030] (4) Safety indicators: No skin allergies, irritation or drug resistance occurred throughout the process, the pass rate of wound microbial testing was 100%, and the medical safety complies with GB 15979 medical and health standards.

[0031] 1. Experimental Model: Liver and kidney of healthy SPF-grade experimental rats were selected as ex vivo organ models to simulate the ex vivo preservation and transplantation of human solid organs. The models were divided into the honey original liquid experimental group and the clinical UW preservation solution control group, with 6 parallel samples in each group.

[0032] 2. Experimental procedure: The experimental group was completely immersed in 42°C sterile purified honey solution with the liquid level 3cm above the tissue, and then sterilely sealed at 4°C for 72 hours; the control group was preserved in the same environment with commercial standard UW preservation solution for 72 hours. After preservation, the tissue activity, physiological structure and oxidative damage indicators were uniformly tested.

[0033] 3. Quantitative Experiment Results: (1) Cell viability: The average viability retention rate of liver and kidney cells in the experimental group was 92.7%, and the apoptosis rate was only 4.1%; the average viability retention rate of cells in the control group was 73.2%, and the apoptosis rate was 22.6%. The cell viability retention rate of the present invention was increased by 26.6%.

[0034] (2) Tissue morphology and structure: Microscopic examination of organ tissue sections in the experimental group showed that the liver lobules and glomeruli were intact and clear, without tissue edema, congestion, vacuolar degeneration, and microvascular walls were intact and lumen was unobstructed; the control group showed obvious edema, interstitial congestion, local cell vacuolar necrosis, and slight spasm and occlusion of microvessels.

[0035] (3) Oxidative stress indicators: The superoxide dismutase (SOD) activity retention rate in the experimental group tissue was 89.3%, and the content of malondialdehyde (MDA) oxidative damage products was only 32.5% of that in the control group, which can significantly scavenge free radicals and inhibit oxidative damage.

[0036] (4) Reperfusion recovery effect: After 72 hours of storage, a transplant reperfusion test was conducted. The average organ revascularization time in the experimental group was 1.2 minutes, the perfusion patency rate was 100%, and there was no obvious ischemia-reperfusion injury. The revascularization in the control group was slow, and 30% of the samples showed perfusion obstruction, local congestion and necrosis.

[0037] 1. Experimental subjects: Skeletal muscle, fascia, blood vessels, and nerve complex soft tissue of the experimental limbs were selected as experimental samples to simulate the scenario of human limb amputation and soft tissue transplantation preservation.

[0038] 2. Experimental conditions: The experimental group was preserved in the sterile honey stock solution of this invention at a constant temperature of 4°C for 48 hours, while the control group was preserved in conventional low-temperature sterile saline. The tissue morphology, vascular patency and nerve cell activity were tested in a uniform manner.

[0039] 3. Quantitative Experiment Results: (1) Tissue morphology: After 48 hours of preservation, the soft tissue in the experimental group was fresh in color and normal in elasticity, without shriveling, mold, or adhesion, and the tissue water content was maintained in the normal physiological range (72%-75%). The tissue water content in the control group was abnormally high, and edema, swelling, and tissue stiffness were observed.

[0040] (2) Vascular and nerve activity: The microvascular patency rate of the experimental group was 96.8%, the nerve cell activity retention rate was 90.2%, and there was no vascular blockage or nerve inactivation. The vascular patency rate of the control group was only 65.3%, and the nerve cell activity loss rate exceeded 40%.

[0041] (3) Prognosis of replantation: In the experimental group, blood flow was smooth after soft tissue replantation of the limbs and the recovery rate of nerve sensory function was excellent in 95%; in the control group, peripheral circulation disorders and slow recovery of nerve function were more likely to occur after replantation, and the incidence of poor prognosis was 48%.

[0042] Under completely identical aseptic environment, temperature, storage time, and tissue sample conditions, the honey preservation system of this invention was quantitatively compared with mainstream clinical UW and HTK solutions from multiple dimensions. The core differences are as follows: 1. Storage duration and cell viability: After 48 hours of storage, the cell viability of the UW solution group decreased to 73.2%, the HTK solution group decreased to 76.5%, while the cell viability of the present invention group remained at 94.5%; after 72 hours of storage, the cell viability of the UW solution group was only 51.8% and tissue degeneration occurred, the HTK solution group was 58.3%, while the cell viability of the present invention group remained stable at over 92%.

[0043] 2. Tissue damage indicators: After 72 hours of preservation, the tissue edema rate in the traditional chemical preservation solution group was 35%-42%, and the incidence of ischemia-reperfusion injury was over 55%; the tissue edema rate in the group of this invention was <5%, and there was no obvious ischemia-reperfusion injury.

[0044] 3. Antibacterial and anti-contamination performance: Traditional preservation solutions have no antibacterial activity, and the incidence of microbial contamination is 18.6% after 72 hours of preservation; this invention relies on the broad-spectrum antibacterial properties of honey, and the contamination rate is 0%.

[0045] 4. Cost comparison: Under the same preservation volume, the cost of using UW liquid and HTK liquid is 28-35 times that of the honey preservation solution of this invention, which has significant advantages in industrialization and clinical application.

[0046] 5. Biocompatibility: Traditional chemical preservation solutions pose a slight risk of immune stimulation, with an incidence of inflammatory reactions after transplantation of 32.7%; the pure natural system of this invention has no immune stimulation, no chemical residues, and an incidence of inflammatory reactions of 0%, with a biocompatibility level far exceeding that of traditional medical preservation media.

Claims

1. A honey-based medical repair preparation and a method for preserving and keeping excised human organs and limbs by immersion, characterized in that... This invention comprises a honey-based thermal burn repair preparation technology system and a sterile honey concentrate human tissue preservation technology system. The honey-based repair preparation is prepared from 80-95 parts of pure natural mature honey with a Baume degree of 41.5° or higher, 3-8 parts of medical petroleum jelly, 1-3 parts of natural propolis extract, 0.5-1.5 parts of licorice extract, and 1-3 parts of sterile distilled water. It is used for anti-inflammatory repair, swelling reduction, pain relief, and scar prevention in the treatment of various thermal burns, scalds, and scalds. The human tissue preservation technology uses sterile honey concentrate with a Baume degree of 41.5° or higher as the sole preservation medium. Through standardized processes such as sterile purification, non-invasive tissue pretreatment, complete immersion and sealing, low-temperature constant-temperature preservation, and preoperative activation and rinsing, it achieves long-term sterile preservation and freshness of human organs, internal organs, severed limbs, and transplanted soft tissues, with no chemical additives, no drug toxicity, and no immune stimulation throughout the entire process.

2. The honey-based medical repair preparation and the method for immersion preservation of excised human organs and limbs according to claim 1, characterized in that, The optimal mass ratio of the repair preparation is: 90 parts of pure natural mature honey with a Baume degree of 42°, 5 parts of medical petroleum jelly, 2 parts of natural propolis extract, 1 part of licorice extract, and 2 parts of sterile distilled water.

3. The honey-based medical repair preparation and the method for immersion preservation of excised human organs and limbs according to claim 1, characterized in that, The preparation steps of the repair agent include: (1) placing honey in a constant temperature environment of 40-45℃ for 20-30 minutes to decrystallize, sterilizing and cooling sterile distilled water under high pressure for later use, and purifying propolis and licorice extract by passing them through a 200-mesh sterile sieve to remove impurities; (2) emulsifying by low-speed constant temperature stirring at 42℃ and 60-80r / min, and fully blending and homogenizing each component; (3) sterilizing at ≤45℃ under ultraviolet light for 15-20 minutes, and then aseptically dispensing and sealing for storage.

4. The honey-based medical repair preparation and the method for immersion preservation of excised human organs and limbs according to claim 1, characterized in that, The application process for burn wounds includes: rinsing the wound with sterile saline and allowing it to drain naturally, without the need for irritating disinfection with iodine or alcohol; applying a 1-2mm thick layer of the preparation evenly, covering the wound and the surrounding 1cm of normal skin; exposing superficial wounds to air dry for repair, and lightly bandaging deep exudative wounds with sterile dressings; changing the dressing every 12 hours for wounds that have not yet scabbed over, and every 24 hours after the scab has stabilized, until the wound is completely healed.

5. The honey-based medical repair preparation and the method for immersion preservation of excised human organs and limbs according to claim 1, characterized in that, The purification process of human tissue-preserved honey stock solution is as follows: Select mature capped honey with a Baume degree of 42°, decrystallize at a constant temperature of 42° for 25 minutes, and sterilize with ultraviolet light at a low temperature of 42° for 18 minutes to prepare a medical sterile stock solution with complete activity.

6. The honey-based medical repair preparation and the method for immersion preservation of excised human organs and limbs according to claim 1, characterized in that, The pretreatment method for ex vivo tissue is as follows: In a Class 10,000 sterile medical environment, the surface of the ex vivo tissue is gently rinsed with sterile physiological saline to remove blood clots, impurities and fragments from the cut surface. After non-invasive cleaning of the wound, the surface liquid is allowed to drain naturally to prevent external contamination and secondary tissue damage.

7. The honey-based medical repair preparation and the method for immersion preservation of excised human organs and limbs according to claim 1, characterized in that, The complete immersion and sealing process involves completely immersing the pretreated isolated tissue in sterile honey solution, with the liquid level 2-3 cm above the top of the tissue, so that the tissue surface, cross-section, and microvascular gaps are completely soaked and wrapped. The tissue is then stored at a constant temperature in a sterile sealed container to isolate it from air.

8. The honey-based medical repair preparation and the method for immersion preservation of excised human organs and limbs according to claim 1, characterized in that, The constant temperature preservation conditions are as follows: the isolated tissue after sealing and soaking is stored in a sterile constant temperature environment at 2-8℃, with the optimal storage temperature being 4℃, which can achieve long-term safe preservation of human tissue for more than 72 hours.

9. The honey-based medical repair preparation and the method for immersion preservation of excised human organs and limbs according to claim 1, characterized in that, The preoperative activation process is as follows: the isolated tissue is removed and repeatedly and gently rinsed with sterile saline to thoroughly remove any residual honey liquid on the tissue surface and in the crevices. After activation, it can be directly used for human organ transplantation, limb replantation, and soft tissue repair surgery.

10. The honey-based medical repair preparation and the method for immersion preservation of excised human organs and limbs according to claim 1, characterized in that, Applicable human ex vivo tissues include: solid organs such as the heart, liver, spleen, kidneys, and lungs; soft tissues of viscera within body cavities; severed intact limbs; skin grafts; fascia; and vascular and nerve grafts.

11. The honey-based medical repair preparation and the method for preserving and keeping excised human organs and limbs by immersion as described in claim 1, characterized in that, By utilizing the natural properties of honey, such as high osmotic pressure for antibacterial, antioxidant, anti-apoptotic, cell membrane stabilization, and improved microcirculation, it can achieve anti-inflammatory and analgesic effects on thermal wounds, rapid healing, and scar repair. It can also inhibit edema and degeneration, cell necrosis, and microbial contamination of human tissues outside the body, effectively prolonging the safe preservation window of tissues outside the body and significantly improving the survival rate of transplantation surgery and the quality of postoperative tissue survival.