A notopterygium dampness-relieving soup traditional Chinese medicine wax therapy dressing, and a preparation method and application thereof

Abstract

The application discloses a Notopterygium Jie-Shi decoction traditional Chinese medicine wax therapy dressing and a preparation method and application thereof. The dressing is prepared by melting and mixing a four-element matrix of beeswax, vaseline, liquid paraffin and oil tea seed oil, and cooling and forming; the beeswax constructs a framework, the vaseline adjusts the consistency, the liquid paraffin is plasticized and extended, and the oil tea seed oil promotes transdermal absorption and synergistic effect. The application also establishes a quality evaluation method combining the heat preservation performance, the ductility, the antibacterial performance, the in-vitro transdermal release and liquid chromatography analysis. The dressing prepared by the application has a clear formula, a stable process and controllable quality, can significantly reduce the joint swelling of a knee osteoarthritis model mouse, and provides a new selection for the treatment of knee osteoarthritis.

Description

Technical Field

[0001] This invention relates to the field of external preparations of traditional Chinese medicine, specifically to a Qianghuo Shengshi Decoction herbal wax therapy dressing, its preparation method, and its application. Background Technology

[0002] Qianghuo Shengshi Decoction originates from *Treatise on Differentiation of Internal and External Injuries* written by Li Dongyuan of the Jin Dynasty. It is an important formula in the *Catalogue of Famous Ancient Prescriptions (First Batch)* published by the State Administration of Traditional Chinese Medicine. Composed of seven herbs—Qianghuo (Notopterygium root), Duhuo (Angelica pubescens root), Gaoben (Ligusticum striatum root), Fangfeng (Saposhnikovia root), Chuanxiong (Ligusticum striatum rhizome), Manjingzi (Vitex trifolia fruit), and Zhigancao (Glycyrrhiza uralensis root)—it has the effects of dispelling wind, eliminating dampness, relieving pain, and releasing the exterior. Modern clinical research has confirmed that Qianghuo Shengshi Decoction and its modified forms are effective in treating wind-cold-dampness syndromes such as cervical spondylosis, frozen shoulder, lumbar disc herniation, and knee osteoarthritis.

[0003] Wax therapy, as a traditional external treatment method in Traditional Chinese Medicine, has a warming effect and a mechanical compression effect, which can significantly dilate local capillaries, accelerate blood circulation, reduce tissue edema, and promote the elimination and absorption of inflammatory products. Combining traditional Chinese medicine with wax therapy can achieve a dual synergistic effect of "medical therapy + physical therapy".

[0004] However, current clinical applications of traditional Chinese medicine (TCM) wax therapy often involve temporary preparation, leading to problems such as inaccurate drug dosage, poor compatibility between the matrix and the drug, and a lack of standardized quality. Existing TCM wax therapies mostly use a single wax matrix, lacking systematic research on optimizing the proportions of different excipients such as beeswax, petrolatum, liquid paraffin, and camellia seed oil. Poor compatibility between raw TCM powders or extracts and the wax matrix can result in uneven dispersion and sedimentation, affecting drug loading uniformity and clinical efficacy.

[0005] As described above, this invention develops a traditional Chinese medicine wax therapy dressing with a clear formula, stable process, and uniform drug loading, providing a new option for the treatment of knee osteoarthritis. Summary of the Invention

[0006] The purpose of this invention is to provide a Qianghuo Shengshi Decoction herbal wax therapy dressing, its preparation method and application. The dressing has a clear formula, stable process and controllable quality, breaking through the limitations of traditional Chinese medicine wax therapy which relies on manual preparation based on experience.

[0007] The present invention provides a Qianghuo Shengshi Decoction herbal wax therapy dressing, comprising a drug-loaded form and a matrix dressing. The drug-loaded form is the original Qianghuo Shengshi Decoction herbal powder, and the matrix dressing comprises beeswax, camellia seed oil, liquid paraffin and petrolatum.

[0008] In the matrix dressing described in this invention, the functions of each component are as follows:

[0009] Beeswax: As the main matrix of wax therapy dressings, its melting point is 62~67℃. Its main function is to provide a warming effect. The large amount of latent heat released during the solidification process of beeswax is the material basis for the warming and therapeutic effects of wax therapy. It can adjust the consistency of the dressing, giving it suitable plasticity and hardness, so that it can remain solid at room temperature. Secondly, beeswax has a certain emulsifying effect, which helps to evenly disperse the components in the drug delivery system.

[0010] Vaseline: Its main function is to adjust the consistency and spreadability, so that the dressing has suitable softness and extensibility, making it easy to apply clinically; it can seal and moisturize, forming a occlusive oil film on the skin surface, reducing water evaporation, enhancing the hydration of the stratum corneum, and promoting transdermal drug absorption; it can improve the skin feel and reduce the hardness and brittleness of beeswax when used alone.

[0011] Liquid paraffin: As a consistency regulator, it adjusts the hardness of the matrix, reduces the viscosity of beeswax and petrolatum, and gives the dressing good fluidity and workability after heating and melting; it can improve the extensibility, so that the dressing retains a certain degree of flexibility after cooling and molding, and is not easy to crack.

[0012] Camellia seed oil: As a functional plant oil matrix, its main function is to promote penetration. Camellia seed oil is rich in unsaturated fatty acids, which can disturb the lipid structure of the stratum corneum and promote drug penetration through the skin barrier. Studies have shown that camellia seed oil has a significant promoting effect on the transdermal penetration of various active ingredients. Secondly, camellia seed oil contains active ingredients such as tea polyphenols and camellia glycosides, which have antibacterial, anti-inflammatory, and antioxidant effects. Camellia seed oil has good skin affinity and is mild and non-irritating. This invention ultimately selects first-grade pressed camellia seed oil, prepared from the mature seeds of Camellia oleifera (a plant in the Theaceae family) using the traditional cold-pressing process, as the matrix oil phase. At the same time, this oil has a refreshing and non-sticky feel, a light odor, and will not interfere with or mask the taste of the medicine, resulting in better patient compliance. In addition, camellia seed oil is cool in nature and good at clearing heat, detoxifying, and reducing swelling. It can neutralize the local heat caused by the warming effect of wax therapy. When combined with Qianghuo Shengshi Decoction, it forms an excellent compatibility characteristic of "warm but not drying, cool but not stagnant," exhibiting synergistic medicinal properties.

[0013] The preparation method of the original medicinal powder of Qianghuo Shengshi Decoction is as follows: weigh out each medicinal material according to 6 parts by weight of Qianghuo, 6 parts by weight of Duhuo, 3 parts by weight of Gaoben, 3 parts by weight of Fangfeng, 3 parts by weight of Zhigancao, 3 parts by weight of Chuanxiong, and 2 parts by weight of Manjingzi. After mixing, pulverize the mixture using a high-speed pulverizer, pass it through a 100-mesh sieve and collect the fine powder.

[0014] The matrix dressing, based on 15g of raw medicinal powder, comprises the following components: 40-60g beeswax, 10-25ml camellia seed oil, 5-20ml liquid paraffin, and 5-20g petrolatum.

[0015] Furthermore, the matrix dressing, based on 15g of raw medicinal powder, comprises the following components: 40-60g beeswax, 10-20ml camellia seed oil, 10-20ml liquid paraffin, and 5-20g petrolatum.

[0016] Furthermore, the matrix dressing, based on 15g of raw medicinal powder, comprises the following components: 50g beeswax, 20ml camellia seed oil, 10ml liquid paraffin, and 5g petrolatum.

[0017] The above-mentioned method for preparing the Qianghuo Shengshi Decoction herbal wax therapy dressing includes the following steps:

[0018] (1) Take the prescribed amount of beeswax, camellia seed oil, liquid paraffin and petrolatum, place them in a container, heat to 60~70℃ and stir to melt and mix well;

[0019] (2) Add the raw medicinal powder of Qianghuo Shengshi Decoction and stir quickly until evenly mixed.

[0020] (3) Pour the mixture into the mold and let it cool and solidify.

[0021] In this invention, beeswax, petrolatum, liquid paraffin, and camellia seed oil constitute a quaternary synergistic matrix system, and the specific synergistic mechanism is as follows:

[0022] (1) Beeswax provides the basic skeletal strength and solidification ability of the dressing; petrolatum gives the dressing suitable adhesion and spreadability; liquid paraffin reduces the viscosity of the system and adjusts the hardness; the polar components such as phospholipids and sterols retained in the first-grade pressed camellia seed oil play a role in crystallization regulation and plasticization. The four components work together to give the dressing good fluidity after heating and suitable hardness, toughness and ductility after cooling.

[0023] (2) Beeswax and petrolatum form a closed oil film on the skin surface, increasing the hydration of the stratum corneum; the unsaturated fatty acids in camellia seed oil further disturb the lipid structure of the stratum corneum, open the drug penetration channels, and significantly enhance the transdermal absorption efficiency of the active ingredients of Qianghuo Shengshi Decoction in the dressing.

[0024] (3) The tea saponins, squalene, tocopherols and sterols retained in the first-grade pressed camellia seed oil have antibacterial effects. The medicinal materials such as Qianghuo, Fangfeng and Chuanxiong in Qianghuo Shengshi Decoction also contain antibacterial active ingredients. Camellia seed oil promotes the migration and dissolution of the effective ingredients of Qianghuo Shengshi Decoction to the surface of the matrix by reducing interfacial tension. The two work together to give the dressing significant antibacterial properties, which can effectively reduce the risk of infection during external application.

[0025] (4) The high latent heat of beeswax provides a long-lasting warming effect. Liquid paraffin and petrolatum regulate the heat conduction rate and prevent heat loss too quickly. The phospholipids, sterols and other polar components retained in the first-grade pressed camellia seed oil can form a fine and uniform crystalline network with beeswax, which improves the latent heat of phase change and crystal density of the system. As a liquid oil, it enhances the uniformity of drug dispersion in the matrix and synergistically achieves a balance between heat preservation performance and drug loading stability.

[0026] This invention systematically combines the classic formula Qianghuo Shengshi Decoction with wax therapy technology to develop a standardized external dressing product with a clear formula, stable process, and controllable quality, breaking through the limitations of traditional Chinese medicine wax therapy which relies on manual preparation based on experience.

[0027] This invention uses a quaternary matrix system of beeswax, petrolatum, liquid paraffin, and camellia seed oil. Through a reasonable ratio, it achieves a balance between heat preservation and handling performance. The dressing has suitable softness, hardness, and extensibility, making it convenient for clinical use. Attached Figure Description

[0028] Figure 1 This is a diagram of the PAMPA penetration model.

[0029] Figure 2 The image shows the liquid chromatogram of the active ingredient standard of Qianghuo Shengshi Decoction.

[0030] Figure 3 The image shows a liquid chromatogram of the extract of dressing 3 prepared in Example 3.

[0031] Figure 4 The image shows a liquid chromatogram of the drug solution in the receiving tube of dressing 3 prepared in Example 3.

[0032] Figure 5 Photos of the circumference of the lower legs of mice before and after applying the medication for modeling.

[0033] Figure 6 The curves showing the changes in leg circumference of mice before and after drug application during modeling. Detailed Implementation

[0034] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the invention.

[0035] The medicinal herbs used in the experiment, including Notopterygium root, Angelica pubescens root, Ligusticum striatum root, Saposhnikovia root, Ligusticum chuanxiong rhizome, Vitex trifolia fruit, and prepared licorice root slices, were all purchased from the medicinal herb market. Chromatographically pure acetonitrile, methanol, formic acid, and ultrapure water were used for high-performance liquid chromatography analysis. The reference standards, notopterygol and osthol, were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. 5-O-methylvisamiloside, purslane, and vitexin were purchased from Shanghai Maclean Biochemical Technology Co., Ltd., with a purity of ≥98%. First-grade pressed camellia seed oil was purchased from Henan Luda Camellia Oil Co., Ltd.; refined camellia seed oil was purchased from Hunan Xianghao Camellia Oil Biotechnology Co., Ltd.; tea seed oil was purchased from Anhui Huayin Camellia Oil Co., Ltd.; olive oil, peanut oil, and sesame oil were all commercially available edible oils.

[0036] In the following embodiments of the present invention, the weight ratio of Notopterygium root, Angelica pubescens root, Ligusticum striatum root, Saposhnikovia divaricata root, prepared licorice root, Ligusticum chuanxiong rhizome, and Vitex trifolia fruit in the Qianghuo Shengshi Decoction is 6:6:3:3:3:3:2. Each medicinal material is weighed according to the weight ratio, mixed, and then pulverized using a high-speed pulverizer. The powder is passed through a 100-mesh sieve and collected; this is the original medicinal powder of the Qianghuo Shengshi Decoction.

[0037] Example 1

[0038] A Qianghuo Shengshi Decoction herbal wax therapy dressing is composed of the following components: 50g beeswax, 15ml first-grade pressed camellia seed oil, 10ml liquid paraffin, 15g petrolatum, and 15g of Qianghuo Shengshi Decoction raw herbal powder.

[0039] The prescribed amount of matrix dressing was placed in a beaker, heated to 65°C, and stirred until melted and homogeneous. 15g of raw medicinal powder was added, and the mixture was stirred rapidly until homogeneous. The mixture was then poured into a mold and cooled to solidify. After preparation, thermal insulation performance, stretchability, antibacterial performance, in vitro transdermal release, and liquid chromatography analysis were performed.

[0040] Example 2

[0041] A herbal wax dressing containing Qianghuo Shengshi Decoction is composed of the following components: 50g beeswax, 20ml first-grade pressed camellia seed oil, 10ml liquid paraffin, 10g petrolatum, and 15g of raw Qianghuo Shengshi Decoction powder. The preparation method is the same as in Example 1.

[0042] Example 3

[0043] A herbal wax dressing containing Qianghuo Shengshi Decoction is composed of the following components: 50g beeswax, 20ml first-grade pressed camellia seed oil, 10ml liquid paraffin, 5g petrolatum, and 15g of raw Qianghuo Shengshi Decoction powder. The preparation method is the same as in Example 1.

[0044] Example 4

[0045] A Qianghuo Shengshi Decoction herbal wax therapy dressing is composed of the following components: 50g beeswax, 10ml first-grade pressed camellia seed oil, 10ml liquid paraffin, 15g petrolatum, and 15g of raw Qianghuo Shengshi Decoction powder. The preparation method is the same as in Example 1.

[0046] Comparative Example 1

[0047] The difference between this comparative example and Example 3 is that the first-grade pressed camellia seed oil is replaced with refined camellia seed oil.

[0048] Comparative Example 2

[0049] The difference between this comparative example and Example 3 is that the first-grade pressed camellia seed oil is replaced with tea seed oil.

[0050] Comparative Example 3

[0051] The difference between this comparative example and Example 3 is that the first-grade pressed camellia seed oil is replaced with olive oil.

[0052] Comparative Example 4

[0053] The difference between this comparative example and Example 3 is that the first-grade pressed camellia seed oil is replaced with sesame oil.

[0054] Comparative Example 5

[0055] The difference between this comparative example and Example 3 is that the first-grade pressed camellia seed oil is replaced with peanut oil.

[0056] Experimental Example 1

[0057] The herbal dressings prepared in each embodiment and comparative example were tested for thermal insulation performance, stretchability, and antibacterial performance.

[0058] (1) Thermal insulation performance test: Specifically, each formula that has been preliminarily screened is expanded to prepare dressings, heated to 65°C to completely melt, and the same mass is accurately measured and poured into molds of the same specifications. A precision thermometer probe is inserted and fixed in the center of the mold, and placed in a constant room temperature environment of 25°C. Timing is started from when the dressing stops heating, and the cooling time from the solidification temperature to 40°C is recorded.

[0059] (2) Extensibility test: Specifically, take an equal amount of dressing sample (about 0.1g), melt it at room temperature, pour it onto a graduated plate, stretch it at a constant speed with a glass rod until it solidifies or breaks, and measure the tensile length of the dressing.

[0060] (3) Antibacterial performance test: Specifically, the antibacterial activity of the medicated dressing was examined using the viable cell count method. The experimental procedure was as follows: single colonies of Staphylococcus aureus and Escherichia coli were picked and inoculated into nutrient broth medium, cultured at 37°C with shaking until the logarithmic growth phase, and then diluted with sterile physiological saline to a concentration of approximately 3 × 10⁻⁶.4 The inoculum was obtained at CFU / mL. 5 mL of inoculum was added to the control tube, and 100 μL of extract was added to 4.9 mL of inoculum in the sample tube. After shaking and incubation, dilution was completed. 100 μL of the original solution, the 10-fold diluted solution, and the 100-fold diluted sample were transferred to nutrient agar plates, spread, and incubated at 37°C for 24-48 hours. Plates with appropriate colony counts were photographed, and the colony counts were calculated.

[0061] Based on the results of thermal insulation performance, ductility performance, and antibacterial performance of all embodiments and comparative examples (see Table 1), Example 3 was determined to be the optimal formulation.

[0062] Table 1. Thermal insulation, stretchability, and antibacterial properties of the Qianghuo Shengshi Decoction wax therapy dressings prepared in each example and comparative example.

[0063]

[0064] Experimental Example 2

[0065] Further in vitro transdermal release experiments were conducted on the optimal formulation of Example 3.

[0066] The preparation process of artificial membrane (PAMPA) is as follows: Figure 1 Specifically, lecithin was dissolved in dodecane to prepare a 5% (w / v) solution. A donor plate (96-well filter plate) was taken, and 10 μL of the above lecithin solution was evenly coated onto the surface of the hydrophilic microporous membrane (0.45 μm pore size) in each well to form a stable keratin-like artificial phospholipid membrane. The coated donor plate was then placed at room temperature for approximately 10 minutes to allow the organic solvent to evaporate.

[0067] The release experiment involved adding 200 μL of PBS buffer (0.01 mol / L, pH 7.4) to each well of the recipient plate as the receiving medium. 150 μL of the extract was accurately measured and added to the corresponding well of the donor plate, ensuring direct contact between the drug and the artificial membrane. The donor plate was then carefully placed over the recipient plate, ensuring full contact between the membrane surface of each donor well and the buffer solution surface in the recipient well. After the assembled PAMPA system was incubated at room temperature for 24 hours, samples were taken from each recipient well.

[0068] The drug assay and data processing involved taking the dressing extract and the drug solution in the receiving tube and using liquid chromatography to determine five index components: notopterygium alcohol, osthol, vitexin, purslane, and 5-O-methylvisamidol. This determined the drug penetration under different dressing formulations. The liquid chromatography conditions were as follows: a C18 column (4.6 mm × 250 mm, 5 μm); mobile phase A was 0.1% formic acid aqueous solution, and mobile phase B was acetonitrile. The gradient elution program was: 0–5 min, 85–80% A; 5–10 min, 80–50% A; 10–13 min, 50–40% A; 13–18 min, 40–35% A; 18–18.01 min, 35–85% A; 18.01–20 min, 85% A; flow rate 1.0 mL / min; column temperature 30℃; injection volume 5 μL.

[0069] Accurately transfer 1 mL each of the dressing extract and the drug solution in the receiving tube, and freeze-dry them at -80℃ to obtain lyophilized powder. Place the lyophilized powder in a stoppered conical flask, add 1 mL of 60% methanol solution, and extract by sonication for 15 min. Take the supernatant and filter it through a 0.22 μm organic microporous membrane to obtain the test solution.

[0070] See results Figures 2-4 The extracts of dressing containing purslane, 5-O-methylvisamidol, and gentianol can be detected by the PAMPA model.

[0071] Experimental Example 3

[0072] A C57 mouse model of knee osteoarthritis was established by intra-articular injection of papain. The traditional Chinese medicine dressing prepared in Example 3 was then applied externally to treat the condition, and the in vivo efficacy of the dressing was evaluated.

[0073] Laboratory animals: SPF-grade female C57 mice, 10 weeks old, weighing (20±2) g, provided by Nanjing Mairis Biotechnology Co., Ltd. All laboratory animals were housed in an environment with a temperature of 22-24℃, relative humidity of 55±5%, and a 12-hour light-dark cycle, with free access to sterilized maintenance feed and purified water. All laboratory animal procedures strictly followed the relevant regulations on laboratory animal ethics of Changzhou University and were approved by the Biomedical Committee of Changzhou University before implementation. Experimental procedures strictly adhered to animal ethical standards.

[0074] Experimental Methods: Healthy 10-week-old female C57 mice were used and acclimatized for 7 days before the experiment. On days 1, 3, and 5 of the experiment, mice were injected intra-articularly into the right knee joint with a mixed solution of 4% papain and 0.03 mol / L L-cysteine ​​at a dose of 0.25 mL / kg. Topical dressing treatment began 48 hours after the last intra-articular injection.

[0075] After melting the medicated dressing by heating it to approximately 42-45°C, the temperature was lowered and it was evenly applied around the right knee joint of mice in the corresponding group, with a thickness of approximately 2 mm, covering the entire knee joint area. A layer of plastic wrap was then used for fixation. Each application lasted approximately 10 minutes, once daily for 7 consecutive days. The mental state, activity level, gait, food and water intake of the mice in each group were observed daily, and their body weight was measured daily. The maximum width of the medial and lateral sides of the right knee joint of each group of mice was measured using a measuring tape before modeling, after modeling, and after treatment to assess the degree of joint swelling. Results are shown below. Figure 5 , Figure 6 .

[0076] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A type of Qianghuo Shengshi Decoction herbal wax therapy dressing, characterized in that, It consists of a drug-loaded form and a matrix dressing. The drug-loaded form is the original medicinal powder of Qianghuo Shengshi Decoction. The matrix dressing consists of beeswax, petrolatum, liquid paraffin and camellia seed oil.

2. The herbal wax therapy dressing for Qianghuo Shengshi Decoction according to claim 1, characterized in that, The camellia seed oil mentioned is first-grade pressed camellia seed oil.

3. The herbal wax therapy dressing for Qianghuo Shengshi Decoction according to claim 1, characterized in that, The original medicinal powder of Qianghuo Shengshi Decoction consists of the following ingredients by weight: 6 parts Qianghuo, 6 parts Duhuo, 3 parts Gaoben, 3 parts Fangfeng, 3 parts Zhigancao, 3 parts Chuanxiong, and 2 parts Manjingzi.

4. The herbal wax therapy dressing for Qianghuo Shengshi Decoction according to claim 3, characterized in that, The original medicinal powder of Qianghuo Shengshi Decoction is obtained by weighing and mixing various medicinal materials, pulverizing them with a high-speed pulverizer, passing them through a 100-mesh sieve, and collecting the fine powder.

5. The herbal wax therapy dressing for Qianghuo Shengshi Decoction according to claim 1, characterized in that, The matrix dressing, based on 15g of raw medicinal powder, comprises the following components: 40-60g beeswax, 10-25ml camellia seed oil, 5-20ml liquid paraffin, and 5-20g petrolatum.

6. The herbal wax therapy dressing for Qianghuo Shengshi Decoction according to claim 5, characterized in that, The matrix dressing, based on 15g of raw medicinal powder, consists of the following components: 40-60g beeswax, 10-20mL camellia seed oil, 10-20mL liquid paraffin, and 5-20g petrolatum.

7. The herbal wax therapy dressing for Qianghuo Shengshi Decoction according to claim 6, characterized in that, The matrix dressing, based on 15g of raw medicinal powder, consists of the following components: 50g beeswax, 20mL camellia seed oil, 10mL liquid paraffin, and 5g petrolatum.

8. A method for preparing a traditional Chinese medicine wax therapy dressing according to any one of claims 1 to 7, characterized in that, The original medicinal powder of Qianghuo Shengshi Decoction is melted and mixed with a base dressing containing beeswax, petrolatum, liquid paraffin and camellia seed oil, and then cooled and shaped to obtain the Qianghuo Shengshi Decoction herbal wax therapy dressing.

9. The preparation method according to claim 8, characterized in that, Includes the following steps: (1) Weigh out the following medicinal materials according to the prescription amount of Qianghuo Shengshi Decoction: Qianghuo, Duhuo, Gaoben, Fangfeng, Zhigancao, Chuanxiong, and Manjingzi. Mix them together, pulverize them, pass them through a 100-mesh sieve, and collect the fine powder to obtain the original medicinal powder of Qianghuo Shengshi Decoction. (2) Take beeswax, petrolatum, liquid paraffin and camellia seed oil according to the prescription, heat to 60~70℃ and stir to melt and mix well; (3) Add the raw medicinal powder prepared in step (1) to the molten matrix in step (2), stir quickly and evenly, pour it into the mold while hot, and cool it at room temperature to form the Qianghuo Shengshi Decoction Chinese herbal wax therapy dressing.

10. The use of the Qianghuo Shengshi Decoction herbal wax therapy dressing according to any one of claims 1 to 7 in the preparation of a topical preparation for treating knee osteoarthritis.

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