Ginkgo biloba leaf shell oligosaccharide capsule for antioxidation and blood fat reduction and preparation method thereof

Abstract

The application belongs to the technical field of health care products, and discloses a ginkgo biloba leaf shell oligosaccharide capsule with antioxidant and blood lipid-lowering functions and a preparation method thereof.The capsule content is prepared from the following raw materials in parts by mass: chitosan 125-145 parts, chitosan oligosaccharide 95-105 parts, ginkgo biloba leaf extract 70-80 parts, grape seed extract 58-62 parts, corn starch 50-60 parts and magnesium stearate 3-7 parts.The application inhibits the absorption of cholesterol through chitosan, promotes lipid metabolism through ginkgo biloba leaf extract, constructs a whole-phase antioxidant network through grape seed extract, and combines water phase, lipid phase and mitochondria, thereby significantly improving the comprehensive effect of blood lipid-lowering and antioxidant, and compared with a traditional single-component capsule, the total cholesterol reduction rate is increased to 25-30%, the DPPH free radical scavenging rate is as high as 90% or more, and the capsule has the functions of immune regulation and cardiovascular protection, thereby meeting the demand of consumers for multifunctional health care products.

Description

Technical Field

[0001] This invention belongs to the field of health product technology, specifically a ginkgo biloba oligosaccharide capsule with antioxidant properties and a method for preparing it to help lower blood lipids. Background Technology

[0002] Chitosan oligosaccharide, also known as chitosan oligosaccharide, is a small-molecule oligosaccharide with amino groups, derived from chitosan derived from shrimp and crab shells. It is a substance with a variety of biological activities and can be widely used in agriculture, food, chemical industry, energy, environmental protection, medicine and other fields. Current research has found that chitosan oligosaccharide plays an important role in human immune regulation, anti-tumor, lowering blood lipids, regulating blood sugar, improving liver and cardiopulmonary function and many other physiological functions.

[0003] However, current chitosan oligosaccharide capsules typically rely on a single active ingredient to regulate blood lipids, lacking a multi-target synergistic mechanism, resulting in low efficiency in lowering blood lipids. At the same time, the high molecular weight of chitosan oligosaccharides in the capsules also leads to poor solubility and absorption, affecting the intestinal absorption and function of chitosan oligosaccharides. Therefore, a chitosan oligosaccharide capsule with multi-component synergistic effects is needed to solve the above problems. Summary of the Invention

[0004] The purpose of this invention is to provide a ginkgo biloba oligosaccharide capsule with antioxidant properties and an auxiliary method for lowering blood lipids, in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a ginkgo biloba oligosaccharide capsule for antioxidant and lipid-lowering purposes and its preparation method, wherein the ginkgo biloba oligosaccharide capsule comprises capsule contents and capsule shell, the capsule contents being made from the following raw materials in parts by weight: 125-145 parts chitosan, 95-105 parts chitosan oligosaccharide, 70-80 parts ginkgo biloba extract, 58-62 parts grape seed extract, 50-60 parts corn starch, and 3-7 parts magnesium stearate, and the capsule shell being made from the following raw materials in parts by weight: 70-80 parts gelatin, 40-50 parts glycerin, 2-5 parts sorbitol, and 20-80 parts water.

[0006] Preferably, the contents of the capsule are a uniform powder, the capsule shell is a hard shell, and the dissolution time is 10-15 minutes.

[0007] Preferably, the preparation process of the capsule is as follows: A1. Weigh each ingredient according to the formula (accuracy ±1%) and remove lumps by passing it through an 80-mesh sieve. A2, using a V-type mixer, first mix the corn starch; A3, then the premix from A2 is mixed with chitosan, chitosan oligosaccharide, ginkgo leaf extract and grape seed extract; A4, finally add magnesium stearate and mix to obtain the capsule contents; A5, the contents of the capsule are filled into the capsule shell using a capsule filling machine, with each capsule containing 500mg.

[0008] Preferably, the preparation process of the ginkgo leaf extract is as follows: B1. First, the ginkgo leaves are screened, washed, and crushed into small pieces of 0.5~1mm. B2 was then extracted three times by reflux with 70% ethanol solution at 80°C. In this extraction step, the amount of ethanol added was 6 times, 4 times, and 4 times, and the time was 3 hours, 2 hours, and 2 hours, respectively. B3 was extracted and then filtered using a 0.45µm microporous membrane to remove fine impurities, resulting in a relatively clear extract. B4. The extract was concentrated under reduced pressure at a low temperature of 50~60°C to 1 / 5 of its original volume. B5, then gradient elution with 70% ethanol is used to separate the target active ingredient from the impurities. At the same time, after collecting the eluent, it is concentrated again under reduced pressure to further remove ethanol and make the extract viscous. B6. Finally, spray drying technology is used. The extract is first atomized into tiny droplets through a spray drying tower, and then dried to form fine ginkgo leaf extract powder.

[0009] Preferably, the preparation process of the grape seed extract is as follows: C1. First, select suitable grape seeds and grind them into 40-60 mesh powder using a grinder. C2, using petroleum ether at 60~90°C as a solvent, grape seed powder is degreased three times. After degreasing, the petroleum ether solution is removed by filtration to obtain degreased grape seed powder. C3. Defatted grape seed powder was added to 80% ethanol and refluxed at 80°C for 1.5 hours. The extract was then filtered and collected, while the residue was retained. C4, repeat C3 three times, and combine the three extracts, then filter them using a 0.45µm microporous membrane; C5 uses a reduced pressure concentration method to concentrate the ethanol-containing extract to 1 / 5 of its original volume, removing most of the ethanol; C6 was then extracted four times with an equal amount of ethyl acetate. Each extraction was carried out with thorough stirring and allowed to stand for separation. The ethyl acetate was then recovered under reduced pressure to obtain a concentrated solution rich in proanthocyanidins. C7. The concentrate is purified by passing it through a polyamide column, and then dried into a powdered extract by passing it through a spray drying tower.

[0010] Preferably, C2 is soaked and stirred for 2-3 hours each time during the degreasing stage, and the ratio of petroleum ether to grape seed powder is 6:1.

[0011] Preferably, in C7, after the concentrate is added to the upper polyamide column, it is first eluted with deionized water to remove impurities, then the water eluent is discarded, and the active ingredient is eluted with 80% ethanol. The eluent is then collected, and finally the ethanol is removed by vacuum evaporation to obtain a high-purity grape seed extract.

[0012] The beneficial effects of this invention are as follows: 1. This invention utilizes chitosan to inhibit cholesterol absorption, ginkgo leaf extract to promote lipid metabolism, and grape seed extract to construct a comprehensive antioxidant network, including aqueous phase, lipid phase, and mitochondria, significantly enhancing the overall effects of lowering blood lipids and antioxidation. Compared with traditional single-ingredient capsules, its total cholesterol reduction rate is increased to 25-30%, and its DPPH free radical scavenging rate is as high as 90% or more. It also has immune regulation and cardiovascular protection functions, meeting consumers' demand for multifunctional health products.

[0013] 2. The capsule shell of the present invention is plasticized by a compound of gelatin, glycerin and sorbitol, which is flexible and stable in the range of -20~40℃, avoiding the brittleness or adhesion problems caused by temperature and humidity changes of traditional capsules. In addition, the contents of the capsule and the shell both meet food safety and pharmacopoeia standards, and are suitable for people with high blood lipids, low immunity and antioxidant needs.

[0014] 3. This invention significantly improves intestinal absorption rate through the optimized combination of high-degree-of-deacetylation chitosan and low-molecular-weight chitosan oligosaccharides. Furthermore, the addition of sorbitol to the capsule shell precisely controls the disintegration time, ensuring efficient release of active ingredients in the intestine and avoiding degradation loss in the gastric acid environment. Attached Figure Description

[0015] Figure 1 This is a flowchart illustrating the capsule preparation process of the present invention. Detailed Implementation The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0016] like Figure 1As shown, this invention provides a ginkgo biloba oligosaccharide capsule for antioxidant and lipid-lowering purposes and its preparation method. The ginkgo biloba oligosaccharide capsule consists of capsule contents and a capsule shell. The capsule contents are made from the following raw materials in parts by weight: 125-145 parts chitosan, 95-105 parts chitosan oligosaccharide, 70-80 parts ginkgo biloba extract, 58-62 parts grape seed extract, 50-60 parts corn starch, and 3-7 parts magnesium stearate. The capsule shell is made from the following raw materials in parts by weight: 70-80 parts gelatin, 40-50 parts glycerin, 2-5 parts sorbitol, and 20-80 parts water.

[0017] Among them, chitosan is used to adsorb bile acids and regulate blood lipids, and should comply with the provisions of SC / T3403 "Chitin and Chitosan"; chitosan oligosaccharide is used for immune regulation and synergistic lipid reduction, and needs to comply with the relevant standards in the "Announcement on Approving Six New Food Raw Materials including Chitosan Oligosaccharide"; corn starch is used as capsule filler and should comply with the relevant standards in the "Pharmacopoeia of the People's Republic of China"; magnesium stearate is used for lubrication and anti-sticking and should comply with the relevant standards of GB 1886.91 "National Food Safety Standard for Food Additives: Magnesium Stearate".

[0018] The capsule shell is plasticized with a compound of gelatin, glycerin and sorbitol, and is flexible and stable within a temperature range of -20~40℃, avoiding the brittleness or adhesion problems caused by temperature and humidity changes in traditional capsules. In addition, both the capsule contents and the shell meet food safety and pharmacopoeia standards, making it suitable for people with high blood lipids, low immunity and antioxidant needs.

[0019] By inhibiting cholesterol absorption with chitosan, promoting lipid metabolism with ginkgo leaf extract, and constructing a comprehensive antioxidant network (aqueous phase + lipid phase + mitochondria) with grape seed extract, the combined effects of lowering blood lipids and antioxidation are significantly enhanced. Compared with traditional single-ingredient capsules, its total cholesterol reduction rate is increased to 25-30%, and its DPPH free radical scavenging rate is as high as 90% or more. At the same time, it also has immune regulation and cardiovascular protection functions, meeting consumers' demand for multifunctional health products.

[0020] The contents of the capsule are a uniform powder, the capsule shell is a hard shell, and the dissolution time is 10-15 minutes.

[0021] The preparation process of this capsule is as follows: A1. Weigh each ingredient according to the formula (accuracy ±1%) and remove lumps by passing it through an 80-mesh sieve. A2. Using a V-type mixer, first mix the corn starch for 15 minutes at a speed of 20 rpm; A3, then mix the premix from A2 with chitosan, chitosan oligosaccharide, ginkgo leaf extract and grape seed extract, mix for 30 minutes at 25 rpm; A4, finally add magnesium stearate and mix for 5 minutes at 10 rpm to obtain the capsule contents; A5, the contents of the capsule are filled into the capsule shell using a capsule filling machine, with each capsule containing 500mg.

[0022] The preparation process of the Ginkgo biloba extract is as follows: B1. First, the ginkgo leaves are screened, washed and crushed to make them into small pieces or coarse powder of 0.5~1mm. B2 was then extracted three times by reflux with 70% ethanol solution at 80°C. In this extraction step, the amount of ethanol added was 6 times, 4 times, and 4 times, and the time was 3 hours, 2 hours, and 2 hours, respectively. B3 was extracted and then filtered using a 0.45µm microporous membrane to remove fine impurities, resulting in a relatively clear extract. B4. The extract was concentrated under reduced pressure at a low temperature of 50~60°C to 1 / 5 of its original volume. B5, then gradient elution with 70% ethanol is used to separate the target active ingredients (flavonoids) from the impurities. At the same time, after collecting the eluent, it is concentrated again under reduced pressure to further remove ethanol and make the extract viscous. B6. Finally, spray drying technology is used. The extract is first atomized into tiny droplets through a spray drying tower, and then dried to form fine ginkgo leaf extract powder.

[0023] During the extraction of ginkgo leaves, flavonoids and terpene lactones are concentrated in the ginkgo leaf extract. Flavonoids can scavenge free radicals and protect vascular endothelial cells, while terpene lactones (≥6%) inhibit platelet aggregation and promote blood circulation in the brain and peripheral tissues.

[0024] The preparation process of the grape seed extract is as follows: C1. First, select suitable grape seeds and grind them into 40-60 mesh powder using a grinder. C2, using petroleum ether at 60~90°C as a solvent, grape seed powder is degreased three times. After degreasing, the petroleum ether solution is removed by filtration to obtain degreased grape seed powder. C3. Defatted grape seed powder was added to 80% ethanol and refluxed at 80°C for 1.5 hours. The extract was then filtered and collected, while the residue was retained. C4, repeat C3 three times, and combine the three extracts, then filter them using a 0.45µm microporous membrane; C5 uses a reduced pressure concentration method to concentrate the ethanol-containing extract to 1 / 5 of its original volume, removing most of the ethanol; C6 was then extracted four times with an equal amount of ethyl acetate. Each extraction was carried out with thorough stirring and allowed to stand for separation. The ethyl acetate was then recovered under reduced pressure to obtain a concentrated solution rich in proanthocyanidins. C7. The concentrate is purified by passing it through a polyamide column, and then dried into a powdered extract by passing it through a spray drying tower.

[0025] During the grape seed extraction stage, proanthocyanidins are concentrated in the grape seed extract, which can target and eliminate free radicals in the blood and inhibit LDL oxidation modification.

[0026] In this process, C2 is soaked and stirred for 2-3 hours each time during the defatting stage, and the ratio of petroleum ether to grape seed powder is 6:1.

[0027] In C7, after the concentrate is added to the upper polyamide column, it is first eluted with deionized water to remove impurities, then the water eluent is discarded, and the active ingredients (mainly proanthocyanidins) are eluted with 80% ethanol. The eluent is then collected, and finally the ethanol is removed by vacuum evaporation to obtain a high-purity grape seed extract.

[0028] To form a powdered extract, drying requires pulverizing the crystals to 80 mesh and then sieving them through a sieve to ensure uniform particle size.

[0029] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0030] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A ginkgo biloba oligosaccharide capsule with antioxidant and lipid-lowering properties, characterized in that: The Ginkgo biloba oligosaccharide capsules consist of capsule contents and capsule shells. The capsule contents are made from the following raw materials in parts by weight: 125-145 parts chitosan, 95-105 parts chitosan oligosaccharide, 70-80 parts ginkgo biloba extract, 58-62 parts grape seed extract, 50-60 parts corn starch, and 3-7 parts magnesium stearate. The capsule shells are made from the following raw materials in parts by weight: 70-80 parts gelatin, 40-50 parts glycerin, 2-5 parts sorbitol, and 20-80 parts water.

2. The Ginkgo biloba oligosaccharide capsule for antioxidant and lipid-lowering effects according to claim 1, characterized in that: The contents of the capsule are a uniform powder, the capsule shell is a hard shell, and the dissolution time is 10-15 minutes.

3. The preparation method of the Ginkgo biloba oligosaccharide capsule with antioxidant and lipid-lowering effects according to claim 1, characterized in that: The preparation process of this capsule is as follows: A1. Weigh each ingredient according to the formula and remove any lumps by passing it through an 80-mesh sieve. A2, using a V-type mixer, first mix the corn starch; A3, then the premix from A2 is mixed with chitosan, chitosan oligosaccharide, ginkgo leaf extract and grape seed extract; A4, finally add magnesium stearate and mix to obtain the capsule contents; A5, the contents of the capsule are filled into the capsule shell using a capsule filling machine, with each capsule containing 500mg.

4. The preparation method of a ginkgo biloba oligosaccharide capsule with antioxidant and lipid-lowering effects according to claim 3, characterized in that: The preparation process of the Ginkgo biloba extract is as follows: B1. First, the ginkgo leaves are screened, washed, and crushed into small pieces of 0.5~1mm. B2 was then extracted three times by reflux with 70% ethanol solution at 80°C. In this extraction step, the amount of ethanol added was 6 times, 4 times, and 4 times, and the time was 3 hours, 2 hours, and 2 hours, respectively. B3, after extraction, was filtered using a 0.45µm microporous membrane to remove fine impurities, resulting in a relatively clear extract; B4. The extract was concentrated under reduced pressure at a low temperature of 50~60°C to 1 / 5 of its original volume. B5, then gradient elution with 70% ethanol is used to separate the target active ingredient from the impurities. At the same time, after collecting the eluent, it is concentrated again under reduced pressure to further remove ethanol and make the extract viscous. B6. Finally, spray drying technology is used. The extract is first atomized into tiny droplets through a spray drying tower, and then dried to form fine ginkgo leaf extract powder.

5. The preparation method of a ginkgo biloba oligosaccharide capsule with antioxidant and lipid-lowering effects according to claim 3, characterized in that: The preparation process of the grape seed extract is as follows: C1. First, select suitable grape seeds and grind them into 40-60 mesh powder using a grinder. C2, using petroleum ether at 60~90°C as a solvent, grape seed powder is degreased three times. After degreasing, the petroleum ether solution is removed by filtration to obtain degreased grape seed powder. C3. Defatted grape seed powder was added to 80% ethanol and refluxed at 80°C for 1.5 hours. The extract was then filtered and collected, while the residue was retained. C4, repeat C3 three times, and combine the three extracts, then filter them using a 0.45µm microporous membrane; C5 uses a reduced pressure concentration method to concentrate the ethanol-containing extract to 1 / 5 of its original volume, removing most of the ethanol; C6 was then extracted four times with an equal amount of ethyl acetate. Each extraction was carried out with thorough stirring and allowed to stand for separation. The ethyl acetate was then recovered under reduced pressure to obtain a concentrated solution rich in proanthocyanidins. C7. The concentrate is purified by passing it through a polyamide column, and then dried into a powdered extract by passing it through a spray drying tower.

6. The preparation method of a ginkgo biloba oligosaccharide capsule with antioxidant and lipid-lowering effects according to claim 3, characterized in that: The C2 is soaked and stirred for 2-3 hours each time during the defatting stage, and the ratio of petroleum ether to grape seed powder is 6:

1.

7. The preparation method of a ginkgo biloba oligosaccharide capsule with antioxidant and lipid-lowering effects according to claim 5, characterized in that: In C7, after the concentrate is added to the upper polyamide column, it is first eluted with deionized water to remove impurities, then the water eluent is discarded, and the active ingredients are eluted with 80% ethanol. The eluent is then collected, and finally the ethanol is removed by vacuum evaporation to obtain a high-purity grape seed extract.

Images